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Littérature scientifique sur le sujet « NanoLC FT MS/MS »

Auteur : Grafiati

Publié le 29 avril 2023

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Articles de revues sur le sujet "NanoLC FT MS/MS"

Umar, Arzu, Theo M. Luider, John A. Foekens et Ljiljana Paša-Tolić. « NanoLC-FT-ICR MS improves proteome coverage attainable for ∼3000 laser-microdissected breast carcinoma cells ». PROTEOMICS 7, n^o 2 (janvier 2007) : 323–29. http://dx.doi.org/10.1002/pmic.200600293.

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Lakshmanan, Rajeswari, Jeremy J. Wolff, Rudy Alvarado et Joseph A. Loo. « Top-down protein identification of proteasome proteins with nanoLC-FT-ICR-MS employing data-independent fragmentation methods ». PROTEOMICS 14, n^o 10 (26 mars 2014) : 1271–82. http://dx.doi.org/10.1002/pmic.201300339.

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Barnes, Stephen, Erin M. Shonsey, Shannon M. Eliuk, David Stella, Kerri Barrett, Om P. Srivastava, Helen Kim et Matthew B. Renfrow. « High-resolution mass spectrometry analysis of protein oxidations and resultant loss of function ». Biochemical Society Transactions 36, n^o 5 (19 septembre 2008) : 1037–44. http://dx.doi.org/10.1042/bst0361037.

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MS, with or without pre-analysis peptide fractionation, can be used to decipher the residues on proteins where oxidative modifications caused by peroxynitrite, singlet oxygen or electrophilic lipids have occurred. Peroxynitrite nitrates tyrosine and tryptophan residues on the surface of actin. Singlet oxygen, formed by the interaction of UVA light with tryptophan, can oxidize neighbouring cysteine, histidine, methionine, tyrosine and tryptophan residues. Dose–response inactivation by 4HNE (4-hydroxynonenal) of hBAT (human bile acid CoA:amino acid N-acyltransferase) and CKBB (cytosolic brain isoform of creatine kinase) is associated with site-specific modifications. FT-ICR (Fourier-transform ion cyclotron resonance)–MS using nanoLC (nano-liquid chromatography)–ESI (electrospray ionization)–MS or direct-infusion ESI–MS with gas-phase fractionation identified 14 4HNE adducts on hBAT and 17 on CKBB respectively. At 4HNE concentrations in the physiological range, one member of the catalytic triad of hBAT (His362) was modified; for CKBB, although all four residues in the active site that were modifiable by 4HNE were ultimately modified, only one, Cys283, occurred at physiological concentrations of 4HNE. These results suggest that future in vivo studies should carefully assess the critical sites that are modified rather than using antibodies that do not distinguish between different modified sites.

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Chew, H. K., S. Miyamoto, H. An, D. Rocke et C. Lebrilla. « Serum glycan analysis in metastatic breast cancer ». Journal of Clinical Oncology 25, n^o 18_suppl (20 juin 2007) : 11504. http://dx.doi.org/10.1200/jco.2007.25.18_suppl.11504.

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11504 Background: There is a need for a reliable breast cancer biomarker that can predict a patient’s response to therapy. Serum glycans, or oligosaccharides, are of particular interest as over half of all proteins are glycosylated and alterations in glycosylation influence growth, adhesion, metastasis and immune surveillance of tumor, among other important functions. Serum glycans can be analyzed by high resolution mass spectrometry. Methods: Sera from patients with known metastatic breast cancer and age-matched healthy controls without medical problems were prospectively analyzed by mass spectroscopy. Women over the age of 18, who were not pregnant or breastfeeding, and who were without other active cancers were eligible. Samples were de-identified for laboratory personnel who analyzed sera by matrix-assisted laser desoprtion/ionization (MALDI) and Fourier transform ion-cyclotron resonance mass sepctrometry (FT ICR MS). Glycans were also profiled by chromatographic separation using a microchip nanoLC (Agilent) with a time-of-flight (TOF) mass analyzers. Results: Sera from 25 patients with metastatic breast cancer and 25 controls were evaluated. The mass profiles were obtained corresponding to both N-linked oligosaccharides (N-glycans) and O-linked oligosaccharides (O-glycans). Distinct variations in glycosylation were observed among sera analyzed from patients with metastatic breast cancer compared to controls. Specific glycan masses were analyzed and found to correspond to N-glycans. The chromatographic glycan profile showed individual glycans that were distinct for the cancer patients. Conclusions: Analysis of serum gylcans by mass spectrometry represents a new paradigm of cancer biomarker studies, focusing on post-translational modifications of proteins, rather than protein expression. Further refinement of this technology may be clinically useful in monitoring response to therapy in metastatic breast cancer. No significant financial relationships to disclose.

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Koch, Heiner, Gary Kruppa et Rohan Thakur. « Plasma Proteomics with NanoLC-MS ». Genetic Engineering & ; Biotechnology News 39, n^o 7 (juillet 2019) : 52–54. http://dx.doi.org/10.1089/gen.39.07.15.

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Stolz, Alexander, et Christian Neusüß. « Characterisation of a new online nanoLC-CZE-MS platform and application for the glycosylation profiling of alpha-1-acid glycoprotein ». Analytical and Bioanalytical Chemistry 414, n^o 5 (9 décembre 2021) : 1745–57. http://dx.doi.org/10.1007/s00216-021-03814-6.

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AbstractThe ever-increasing complexity of biological samples to be analysed by mass spectrometry has led to the necessity of sophisticated separation techniques, including multidimensional separation. Despite a high degree of orthogonality, the coupling of liquid chromatography (LC) and capillary zone electrophoresis (CZE) has not gained notable attention in research. Here, we present a heart-cut nanoLC-CZE-ESI-MS platform to analyse intact proteins. NanoLC and CZE-MS are coupled using a four-port valve with an internal nanoliter loop. NanoLC and CZE-MS conditions were optimised independently to find ideal conditions for the combined setup. The valve setup enables an ideal transfer efficiency between the dimensions while maintaining good separation conditions in both dimensions. Due to the higher loadability, the nanoLC-CZE-MS setup exhibits a 280-fold increased concentration sensitivity compared to CZE-MS. The platform was used to characterise intact human alpha-1-acid glycoprotein (AGP), an extremely heterogeneous N-glycosylated protein. With the nanoLC-CZE-MS approach, 368 glycoforms can be assigned at a concentration of 50 μg/mL as opposed to the assignment of only 186 glycoforms from 1 mg/mL by CZE-MS. Additionally, we demonstrate that glycosylation profiling is accessible for dried blood spot analysis (25 μg/mL AGP spiked), indicating the general applicability of our setup to biological matrices. The combination of high sensitivity and orthogonal selectivity in both dimensions makes the here-presented nanoLC-CZE-MS approach capable of detailed characterisation of intact proteins and their proteoforms from complex biological samples and in physiologically relevant concentrations. Graphical abstract

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McClung, Colleen, Hang Gyeong Chin, Ulla Hansen, Christopher J. Noren, Sriharsa Pradhan et Cristian I. Ruse. « Mapping of polyglutamylation in tubulins using nanoLC-ESI-MS/MS ». Analytical Biochemistry 612 (janvier 2021) : 113761. http://dx.doi.org/10.1016/j.ab.2020.113761.

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Bag, Swarnendu, Debabrata Dutta, Amrita Chaudhary, Bidhan Chandra Sing, Rita Banerjee, Mousumi Pal, Ranjan Rashmi Paul et al. « NanoLC MALDI MS/MS based quantitative metabolomics reveals the alteration of membrane biogenesis in oral cancer ». RSC Advances 6, n^o 67 (2016) : 62420–33. http://dx.doi.org/10.1039/c6ra07001a.

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Li, Haiying, Stephen A. Kostel, Shannon E. DiMartino, Ali Hashemi Gheinani, John W. Froehlich et Richard S. Lee. « Uromodulin Isolation and Its N-Glycosylation Analysis by NanoLC-MS/MS ». Journal of Proteome Research 20, n^o 5 (2 mars 2021) : 2662–72. http://dx.doi.org/10.1021/acs.jproteome.0c01053.

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Boutin, Michel, Carl Berthelette, François G. Gervais, Mary-Beth Scholand, John Hoidal, Mark F. Leppert, Kevin P. Bateman et Pierre Thibault. « High-Sensitivity NanoLC−MS/MS Analysis of Urinary Desmosine and Isodesmosine ». Analytical Chemistry 81, n^o 5 (mars 2009) : 1881–87. http://dx.doi.org/10.1021/ac801745d.

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Thèses sur le sujet "NanoLC FT MS/MS"

Camperi, Julien. « Développement de méthodes séparatives pour la caractérisation d’une glycoprotéine intacte : application à l’hormone chorionique gonadotrophine humaine ». Thesis, Paris Sciences et Lettres (ComUE), 2018. http://www.theses.fr/2018PSLET018.

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La glycosylation est la forme la plus courante de modification post-traductionnelle (PTM) des protéines humaines, puisque plus de 70% d’entre elles sont glycosylées. Celle-ci régule de nombreuses propriétés biologiques comme leur stabilité, leur demi-vie et leur activité. Néanmoins, les protéines peuvent également présenter d'autres types de PTM, ce qui peut conduire pour une protéine donnée à un très grand nombre d'isoformes variant par leur masse, leurs propriétés biologiques et physico-chimiques et leur concentration dans les échantillons biologiques. Ainsi, caractériser une glycoprotéine comporte de nombreux défis et nécessite la mise en œuvre de méthodes séparatives très performantes et de détection très sensibles et informatives.La gonadotrophine chorionique humaine (hCG) est l’hormone spécifique de la grossesse humaine. Elle est essentielle au développement du placenta et du fœtus. Elle est composée de deux sous-unités hCGα et hCGβ qui sont fortement glycosylées (4 sites de N-glycosylation et 4 sites d’O-glycosylation). Récemment, des travaux ont montré une corrélation entre sa glycosylation et une bonne implantation du fœtus. Une caractérisation des ces glycoformes s’avère donc nécessaire.Par conséquent, de nouvelles méthodes en LC/CE-MS ont été développées pour la caractérisation de la hCG à l’échelle intacte en utilisant deux médicaments à base de hCG ayant des glycosylations différentes. Alors que la méthode en CZE-MS (TQ) a permis de différencier les profils des glycoformes de la sous-unité hCGα des deux médicaments, la complémentarité des méthodes RP- et HILIC-MS (qTOF) a conduit à leur identification.Pour limiter les erreurs potentielles d’identification dues au chevauchement des profils isotopiques, le profil de chaque isoforme a été résolu par FT-ICR MS. Dans ce but, une séparation au format nanoLC en mode RP a été développée, améliorant ainsi la sensibilité de la méthode d’un facteur 500 par rapport au format conventionnel. Cette méthode a permis de confirmer l’identification des glycoformes de la sous-unité hCGα. D’autre part, il a été possible d’obtenir des profils différents de glycosylation de la sous-unité hCGβ en favorisant leur ionisation par réduction de la hCG. Enfin, un traitement à la PNGase a conduit à l’élimination des N-glycanes pour l’obtention des profils d’O-glycosylation de la sous-unité hCGβ
Glycosylation is the most common form of post-translational modifications (PTMs) of human proteins, since more than 70% are glycosylated. It regulates numerous biological properties including their stability, half-life, and activity. Nevertheless, proteins can also exhibit other types of PTMs that lead to a very large number of isoforms, varying in mass, properties and concentration in the biological samples. Therefore, the characterization of a glycoprotein is highly challenging and requires the use of powerful separation techniques and sensitive and informative detection modes.The human chorionic gonadotropin (hCG) is the hormone specific to human pregnancy. It is essential for the development of placenta and fetus. It is based on two heavily glycosylated subunits, hCGα and hCGβ, having 8 glycosylation sites (4 N- and 4 O-glycosylation sites). Some recent studies demonstrated that here is a correlation between the hCG glycosylation state and the fetus implantation. This is why the characterization of the hCG glycoformes is needed.Therefore, new LC/CE-MS methods were developed for the characterisation of hCG at the intact level using two hCG-based drugs having different glycosylation profiles. While the CZE-MS (TQ) method showed its potential for glycosylation fingerprinting, the complementarity of LC-(qTOF) MS methods in RP and HILIC modes allowed the identification of the glycoforms of the hCGα subunit.To limit the identification errors due to the overlapping of isotopic distribution patterns, the profile of each isoform was resolved by FT-ICR MS. For this purpose, a nanoLC separation in RP mode was developed, thus improving the sensitivity of the method by a factor 500 compared to the conventional format. This method allowed the confirmation of the identification of hCGα glycoforms. Then, it was possible to obtain different glycosylation patterns of the hCGβ by promoting its ionization after hCG reduction. Then, a PNGase treatment was carried out to remove the N-glycans in order to obtain the O-glycoprofiles of hCGβ isoforms

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Emmanuel, Alexandra. « Mise en place d’une approche protéomique en vue d’une application au diagnostic d’infections parasitaires congénitales ». Electronic Thesis or Diss., Sorbonne Paris Cité, 2017. http://www.theses.fr/2017USPCB137.

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Au cours des deux dernières décennies, la spectrométrie de masse (MS) est devenue un outil incontournable dans la caractérisation des protéines comme les IgG. Ces anticorps assurent la protection de l'organisme contre les antigènes, qu'ils reconnaissent grâce aux domaines variables de leurs chaînes lourdes et légères. Des séquences polymorphes existent également au niveau des domaines constants CH2 et CH3 du fragment Fc, impliquant quelques acides aminés, qui définissent ainsi 34 allèles protéiques IGHG des 4 sous-classes d'IgG. Des relations sont établies entre certains de ces polymorphismes et la fonctionnalité de la réponse anticorps. La caractérisation du polymorphisme allélique des IgG par la MS est fréquemment effectuée par la stratégie bottom-up. Cette approche analytique repose sur l'analyse des protéines digérées par des endoprotéases classiques (comme la trypsine) générant des peptides protéolytiques (~30 acides aminés). Nous avons donc utilisé cette méthode combinée à des approches de biologie moléculaire pour étudier ce polymorphisme. Cependant la présence, pour un même individu, de plusieurs allèles d'homologie très forte limite la caractérisation spécifique des séquences de Fc. Dans ce contexte, nous avons développé une approche middle-down permettant d'analyser de grands fragments peptidiques (~ 211 acides aminés). IdeS est une endoprotéase spécifique des IgG qui génère spécifiquement deux fragments : les fragments Fc/2 et F(ab')2. Ces fragments sont alors étudiés par nanoLC-MS/MS à très haute résolution (140000 à m/z 200). La fragmentation des ions précurseurs a été réalisée en mode HCD (higher-energy collisional dissociation). Les données obtenues ont été traitées pour recherche dans les banques de données et les résultats ont été validés manuellement. Malgré la faible performance des logiciels disponibles à ce jour pour automatiser ce traitement, notre approche montre qu'il est possible de discriminer plusieurs allèles IGHG des différentes IgG. Au vu de la forte homologie de séquence il est important d'optimiser la couverture de séquence car certains allèles ne diffèrent que d'un résidu sur les 211 acides aminés séquencés. Cette preuve de concept des capacités d'une analyse middle-down pour identifier les différents Fc/2 pourrait à l'avenir être utilisée comme outil diagnostic pour la caractérisation des polymorphismes des Fc/2 dans un contexte de suspicion d'infections parasitaires congénitales
Over the past two decades, mass spectrometry (MS) has become an essential tool in the characterization of proteins such as IgG. These antibodies protect the organism against antigens, which they recognize through the variable domains of their heavy and light chains. Polymorphic sequences also exist at the constant domains CH2 and CH3 of the Fc fragment, involving a few amino acids, which thus define 34 IGHG protein alleles of the 4 IgG subclasses. Relationships are established between some of these polymorphisms and the functionality of the antibody response. The characterization of allelic polymorphism of IgG by MS is frequently carried out by the bottom-up strategy. This analytical approach relies on the analysis of proteins digested by conventional endoproteases (such as trypsin) generating proteolytic peptides (~ 30 amino acids). We have therefore used this method combined with molecular biology approaches to study this polymorphism. However, the presence, for the same individual, of several alleles of very strong homology limits the specific characterization of the sequences of Fc. In this context, we developed a middle-down approach to analyze large peptide fragments (~ 211 amino acids). IdeS is an IgG-specific endoprotease that specifically generates two fragments: the Fc/2 and F (ab')2 fragments. These fragments are then studied by nanoLC-MS/MS at very high resolution (140000 at m/z 200). The fragmentation of the precursor ions was carried out in HCD (higher-energy collisional dissociation) mode. The data obtained were processed and searched in the databases and the results were validated manually. Despite the poor performance of the software available to date due to limited automation, our approach shows that it is possible to discriminate several IGHG alleles from different IgGs. In view of the high sequence homology it is important to optimize the sequence coverage because some alleles differ only by one residue over the 211 amino acids sequenced. This proof of concept of the capabilities of a middle-down analysis to identify different Fc/2 could be used in the future as a diagnostic tool for the characterization of Fc /2 polymorphisms in a context of suspicion of congenital parasitic infections

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Danne-Rasche, Niklas [Verfasser], et Oliver [Akademischer Betreuer] Schmitz. « Development of a nanoLC-MS system for global lipidomics / Niklas Danne-Rasche ; Betreuer : Oliver Schmitz ». Duisburg, 2021. http://d-nb.info/1232176036/34.

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Mitchell, Joshua Merritt. « Computational Tools for the Untargeted Assignment of FT-MS Metabolomics Datasets ». UKnowledge, 2019. https://uknowledge.uky.edu/biochem_etds/42.

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Metabolomics is the study of metabolomes, the sets of metabolites observed in living systems. Metabolism interconverts these metabolites to provide the molecules and energy necessary for life processes. Many disease processes, including cancer, have a significant metabolic component that manifests as differences in what metabolites are present and in what quantities they are produced and utilized. Thus, using metabolomics, differences between metabolomes in disease and non-disease states can be detected and these differences improve our understanding of disease processes at the molecular level. Despite the potential benefits of metabolomics, the comprehensive investigation of metabolomes remains difficult. A popular analytical technique for metabolomics is mass spectrometry. Advances in Fourier transform mass spectrometry (FT-MS) instrumentation have yielded simultaneous improvements in mass resolution, mass accuracy, and detection sensitivity. In the metabolomics field, these advantages permit more complicated, but more informative experimental designs such as the use of multiple isotope-labeled precursors in stable isotope-resolved metabolomics (SIRM) experiments. However, despite these potential applications, several outstanding problems hamper the use of FT-MS for metabolomics studies. First, artifacts and data quality problems in FT-MS spectra can confound downstream data analyses, confuse machine learning models, and complicate the robust detection and assignment of metabolite features. Second, the assignment of observed spectral features to metabolites remains difficult. Existing targeted approaches for assignment often employ databases of known metabolites; however, metabolite databases are incomplete, thus limiting or biasing assignment results. Additionally, FT-MS provides limited structural information for observed metabolites, which complicates the determination of metabolite class (e.g. lipid, sugar, etc. ) for observed metabolite spectral features, a necessary step for many metabolomics experiments. To address these problems, a set of tools were developed. The first tool identifies artifacts with high peak density observed in many FT-MS spectra and removes them safely. Using this tool, two previously unreported types of high peak density artifact were identified in FT-MS spectra: fuzzy sites and partial ringing. Fuzzy sites were particularly problematic as they confused and reduced the accuracy of machine learning models trained on datasets containing these artifacts. Second, a tool called SMIRFE was developed to assign isotope-resolved molecular formulas to observed spectral features in an untargeted manner without a database of expected metabolites. This new untargeted method was validated on a gold-standard dataset containing both unlabeled and 15N-labeled compounds and was able to identify 18 of 18 expected spectral features. Third, a collection of machine learning models was constructed to predict if a molecular formula corresponds to one or more lipid categories. These models accurately predict the correct one of eight lipid categories on our training dataset of known lipid and non-lipid molecular formulas with precisions and accuracies over 90% for most categories. These models were used to predict lipid categories for untargeted SMIRFE-derived assignments in a non-small cell lung cancer dataset. Subsequent differential abundance analysis revealed a sub-population of non-small cell lung cancer samples with a significantly increased abundance in sterol lipids. This finding implies a possible therapeutic role of statins in the treatment and/or prevention of non-small cell lung cancer. Collectively these tools represent a pipeline for FT-MS metabolomics datasets that is compatible with isotope labeling experiments. With these tools, more robust and untargeted metabolic analyses of disease will be possible.

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Vaz, Boniek Gontijo. « Petroleômica por FT-ICR MS = desvendando a composição de polares do petróleo e derivados ». [s.n.], 2011. http://repositorio.unicamp.br/jspui/handle/REPOSIP/248707.

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Orientador: Marcos Nogueira Eberlin
Tese (doutorado) - Universidade Estadual de Campinas, Instituto de Química
Made available in DSpace on 2018-08-17T19:44:03Z (GMT). No. of bitstreams: 1 Vaz_BoniekGontijo_D.pdf: 5976612 bytes, checksum: 09cb6bf00d2c85b09a8ad20578b0ac32 (MD5) Previous issue date: 2011
Resumo: A ionização por eletrospray (ESI) acoplada com a espectrometria de massas de ressonância ciclotrônica de íons por transformada de Fourrier (FT-ICR MS) permite a detecção de milhares de compostos polares (N, S e O) no petróleo e seus derivados, sem separação cromatográfica. ¿Petroleômica¿ é, portanto, a caracterização do petróleo a nível molecular. Neste trabalho, petróleos de diferentes níveis de evolução térmica, amostras de diesel obtidas por processos diferentes (hidrotratamento e oxitratamento) e cortes de destilação obtidos da destilação molecular de resíduo de petróleo foram analisados usando esta técnica. A primeira parte do trabalho consistiu na análise de petróleos com diferentes níveis de evolução térmica. Com o aumento do estresse térmico, observou-se um aumento da condensação, da aromatização de estruturas cíclicas polares e uma diminuição do número de carbono das classes de compostos identificadas. A intensidade relativa dos compostos pirrólicos foi usada para agrupar os óleos de mesmo nível de evolução térmica usando Análise dos Componentes Principais (PCA), que mostrou bastante eficiente. Na segunda parte, a análise de amostras de diesel obtidas por condições operacionais diferentes (temperatura, pressão e velocidade espacial) do hidrotratamento e pelo processo de oxitratamento, mostrou a produção, respectivamente, de espécies de baixo valores de DBE (do inglês Double Bond Equivalent), devido a hidrogenação dos anéis aromáticos presentes nos compostos polares, e uma variedade de classes contendo mais que um heteroatômo, como Ox ,NOx and SOx, devido a oxidação química dos hidrocarbonetos e compostos polares. A última parte consistiu na análise dos cortes de destilação. Evidenciaram-se com a caracterização de cada corte de destilação as tendências composicionais com aumento da temperatura de destilação. O peso molecular, aromaticidade, o conteúdo de heteroátomos e a distribuição do número de carbono aumentaram proporcionalmente a temperatura de destilação. Os resultados mostraram que é possível a partir dos milhares de compostos, identificados rotineiramente por FT-ICR MS, predizer as propriedades do petróleo e seus derivados, como também na otimização de processos como aqueles que reduzem o nível dos compostos NOS
Abstract: Electrospray ionization (ESI) coupled with Fourier transform ion cyclotron resonance mass spectrometry (FT-ICR MS) allows the direct detection of thousands of polar compounds (N, S and O) in petroleum and its derivatives, without chromatographic separation. ''Petroleomics¿¿ is therefore the characterization of petroleum at the molecular level. In this work, oils from different levels of thermal maturity, diesel fuel obtained by different processes (hydrotreatment and oxitreatment) and distillate cuts obtained from molecular distillation of petroleum residue were analyzed using this technique. The first part of this study consisted in the analysis of oils of different levels of thermal maturity. With increasing thermal stress, condensation and aromatization of the polar cores increase, and the number of alkyl carbons decreases. The relative intensity of the pyrrolic compounds were used for grouping the oils with same level of thermal maturity by Principal Analysis Components (PCA), that shown to be quite efficient. In a second step, the analysis of different diesel fuel oil samples, obtained from different operational conditions (temperature, pressure and liquid hourly space velocity) of hydrotreatment and by oxitreatment process, showed the production, respectively, of lower DBE (Double Bond Equivalent) species, due the hydrogenation of the aromatic rings present in the polar compounds, and a variety of classes containing more than one heteroatom, such as Ox ,NOx and SOx, due the chemical oxidation of hydrocarbons and heteroatom compounds present in these fuel. The last stage consisted in the analysis of distillate cuts. Each distillate cut was characterized to mark compositional trends with increased distillation temperature. The molecular weight, aromaticity, heteroatom content and carbon number distribution increase proportionately with the temperature. The results show that it is possible from the thousands compounds, identified routinely by FTICR MS analysis, predict the properties of petroleum and its derivatives, as well help improve processes such that used for reduce the level of NSO compounds
Doutorado
Quimica Organica
Doutor em Ciências

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Boutegrabet, Lemia. « Approche métabolomique dans l'analyse de l'évolution oxydative des vins en spectrométrie de masse à très haute résolution ». Thesis, Dijon, 2012. http://www.theses.fr/2012DIJOS025.

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Tout au long du procédé d’élaboration d’un vin, des réactions d’oxydation peuvent se produire y compris au cours du vieillissement en bouteilles. Depuis quelques années, la profession viti-vinicole est confrontée au problème de l’oxydation prématurée des vins blancs.. A ce jour, peu d’études ont pu apporter des explications d’ordre chimique à ce phénomène, et les mécanismes réactionnels intervenant restent peu connus.L’objectif de ce travail de thèse est d’apporter, au travers d’une analyse moléculaire non ciblée en spectrométrie de masse à transformée de Fourier et à résonance cyclotronique des ions (FT-ICR-MS) couplée à une étude chimiométrique, des réponses originales aux questions posées par la problématique actuelle d’oxydation prématurée des vins blancs. Nous avons montré suite à l’étude d’une série de vins oxydés prématurément non seulement la grande diversité chimique des vins, mais aussi la présence d’un ensemble de masses typiques associées à ce phénomène. Pour une meilleure compréhension de l’origine de cette problématique, nous avons considéré deux autres types d’oxydation : une oxydation relative exclusivement à un apport contrôlé en oxygène et une autre relative à l'évolution naturelle de vins en bouteilles. Cette dernière consiste en le suivi de l'évolution des espaces chimiques de séries verticales de vins blancs et rouges en fonction du temps. Sur la série verticale des vins blancs allant de 1979 à 2006, une charnière à l’année 1990 a été observée avec des groupes de masses typiques de chacun des vins jeunes (1979-1990) et des vieux vins (1991-2006).La comparaison entre les espaces chimiques discriminants chacun de ces trois types d’oxydation ne révèle la présence que de trois masses en commun, ce qui appuie l'hypothèse de causes multiparamétriques à l’oxydation prématurée des vins blancs, qui ne serait donc pas un phénomène du exclusivement à une exposition non contrôlée à l'oxygène.Des essais d’élucidation structurale en FT-ICR-MS/MS des masses discriminantes des vins oxydés et de la série verticale ont été effectués et des schémas de fragmentation pour certaines masses sont proposés
During winemaking processes, many oxidation reactions may occur especially during the aging period. Recently, white wines are characterized by a problem of premature oxidation for which few studies have provided chemical explanation. To date, the involved mechanisms in this phenomenon remain poorly understood.The aim of this thesis project is to provide, through an untargeted molecular analysis using Fourier transform ion cyclotron resonance mass spectrometry (FT-ICR-MS) coupled to chemometric analysis, original clue to understand the premature oxidation of white wines. Based on the study of a series of premature oxidized white wines, we were able to elucidate the high complexity and the chemical diversity of wine, and got out typical masses characterizing the oxidation state. In order to better understand the origin of this phenomenon, we considered two alternative possibilities of oxidation: the first one induced by oxygen, and the second through a natural evolution of wines in bottles. The latter included the monitoring of the chemical evolution of white and red wines as a function of time. A very interesting result was obtained on the vertical series of white wines from 1979 to 2006, where two groups were separated at the 1990 vintage to provide a group of old wines (1979-1990) and a group of new wines (1991-2006). Typical discriminant masses were found for each group.A comparison between the chemical spaces discriminating each of the three types of oxidation (premature oxidation, oxidation with oxygen and natural evolution of wine in bottle) revealed very few common masses that may indicate that the phenomenon of premature oxidation is indeed influenced by multiple factors.Finally, a structural elucidation of the typical masses of the groups of oxidized and aged wines were established using FT-ICR-MS/MS. Possible fragmentations schemes of some of these masses were proposed

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Brunel, Benoît. « Détermination des interactions microscopiques entre les actinides and les substances humiques ». Thesis, Paris 11, 2015. http://www.theses.fr/2015PA112024/document.

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Détermination des interactions microscopiques entre les actinides et les substances humiques présentes dans l’environnementUne quantité pondérable de plutonium a été introduite dans l’environnement lors d’essais nucléaires ou d’accidents civils. Les zones contaminées, nécessitant une surveillance particulière, sont devenues des lieux d’études très intéressants pour comprendre le comportement du plutonium dans l’environnement. Jusqu’à peu de temps encore, il était admis que le plutonium introduit dans les sols était relativement immobile en raison de sa propension à se sorber fortement sur les roches et de sa faible solubilité dans l’eau. Cependant, des études menées sur des zones contaminées montrent que les substances humiques, omniprésentes dans l’environnement, peuvent influencer la spéciation d’un métal et donc sa migration. C’est pourquoi, il est indispensable de connaître l’influence des substances humiques sur la spéciation du plutonium pour comprendre le transport de celui-ci dans un milieu naturel. Toutefois, en raison de la nature complexe et hétérogène de la matière organique naturelle, il est difficile de décrire de façon microscopique les interactions entre substances humiques et le plutonium. En effet, les substances humiques forment un mélange hétérogène de supra-molécules polydispersées produites par des réactions chimiques ou biochimiques durant la transformation naturelle de matière organique animale, végétale ou microbienne.Le but de ces travaux est d’évaluer à l’échelle de moléculaire les phénomènes de complexation des actinides par la matière organique naturelle. Ces recherches s’organisent autour de deux axes : la caractérisation de dizaines de milliers de molécules dans chaque échantillon de substances humiques naturelles et la détermination des molécules responsables de la vectorisation des actinides dans l’environnement.La mise au point d'une méthodologie a permis de mettre en évidence, pour la première fois, quelques molécules responsables de la complexation des actinides dans des échantillons de matière organique naturelle. Les résultats obtenus montrent l’influence de l’origine du prélèvement de la matière organique sur le type de molécules complexantes. Cette étude montre, également, que la vectorisation des actinides par la matière organique résulte d’une interaction avec un ensemble de molécules présentant des affinités plus ou moins forte pour le métal. Ces résultats semblent être en accord avec les modélisations macroscopiques NICA-DONNAN (approches continue) utilisés actuellement
Large amount of plutonium has been introduced into the environment as a result of nuclear weapons testing, and nuclear power-plant accidents. Contaminated areas, which need a particular survey, have become a very interesting place to study and understand the plutonium behaviour in the environment. Until few years ago, it was admitted that plutonium introduced into subsurface environment is relatively immobile, owing to its low solubility in ground water and strong sorption onto rocks. However, studies of contaminated areas show that humic substances, which are ubiquitous in environment, can alter the speciation of metal ion, e.g. plutonium, and thus their migration. These humic substances are major components of the natural organic matter in soil and water as well as in geological organic deposits such as lake sediments, peats and brown coals. They are complex heterogeneous mixtures of polydispersed supra-molecules formed by biochemical and chemical reactions during the decay and transformation of plant and microbial remains. The knowledge of the impact of humic substances on the plutonium migration is required to assess their transport in natural systems. However, due to the complex and heterogeneous nature of humic substances, there are a lot of difficulties in the description of microscopic interactions. The aim of this PhD thesis is to evaluate as precisely as possible interactions between actinides and humic substances. This work is divided in two parts: on the one hand humic substances will be separated to identify each component, on the other hand the speciation of actinides with characterized humic substances will be studied. In the first part of this study, new methods are developed to study the speciation of actinides with humic substances using two kinds of mass spectrometers: an ICP-MS and a high resolution mass spectrometer using various ionization devices (ESI, APCI, DART, APPI) in order to determine all active molecules for the complexation.In the second part, a new way of humic substances separation had been developed using an electrophoresis capillary apparatus. This efficient separation technique allows to simplify the characterisation of the complex heterogeneous mixtures without changing speciation of the chemical system.This study will lead to point out some molecules responsible for complexation of actinides by the humic substances

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Nickum, Elisa A. « Analysis of Regulated Drugs Using Chromatographic and Spectrophotometric Techniques Coupled with Spectroscopy An Orthogonal Approach to Protecting Public Health ». University of Cincinnati / OhioLINK, 2017. http://rave.ohiolink.edu/etdc/view?acc_num=ucin1504799568245931.

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Neumaier, Marco. « FT-ICR-MS Studien zur Reaktivität kationischer Münzmetallcluster und zur elektronischen Stabilität von Fullerenanionen ». Karlsruhe Forschungszentrum Karlsruhe, 2007. http://d-nb.info/989564908/34.

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Vasconcelos, Géssica Adriana. « Estendendo a caracterização dos compostos nitrogenados em resíduo de vácuo por FT-ICR MS ». Universidade Federal de Goiás, 2016. http://repositorio.bc.ufg.br/tede/handle/tede/5952.

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Petroleomics by FT-ICR MS was able to characterize petroleum and its derivatives at molecular level. The most used ionization source on the analysis of these compounds is electrospray. However, species that are more basic or more acidic suppress the ionization of other compounds with less strength. This disadvantage consists of, especially in positive mode, only detecting compounds with higher pKa values preventing the mass spectrometer from reading less basic species that is also present in the sample. In view of this, the development of new methods whose purpose is to minimize these effects is essential. An alternative is to use fast techniques of extraction and separation followed by analysis by FT-ICR MS. This study pursued two goals: first, to perform an extraction of vacuum residue into basic and neutral nitrogen compounds using different methods, and second, to fractionate the basic nitrogen compounds present in RV according to their mass/ratio. Three methods were tested to achieve the first objective: solvent extraction (LLE), column chromatography (LC), and solid phase extraction (SPE). The results showed that LLE was the less selective technique among all three studied procedures. SPE and LC showed similar results, and they allowed to partially separate the basic nitrogen compounds from neutral species. To accomplish the second goal, a fractionation using SPE was applied to the vacuum residue sample. This procedure permitted to detect compounds that apparently were not present in the untreated sample as a consequence of a reduction on ion suppression effect. Electrospray ionization and high resolution mass spectrometry (FT-ICR MS) were employed on the analysis of each sample.
A petroleômica por FT-ICR MS foi capaz de caracterizar o petróleo e seus diferentes derivados a nível molecular. A fonte de ionização mais utilizada na análise destes compostos é o electrospray. Componentes mais básicos e mais ácidos, entretanto, suprimem a ionização de outras espécies com menor força. A grande desvantagem deste fato consiste, especialmente no modo positivo, detectar somente os compostos com elevado pKa impedindo a leitura das espécies menos básicas presentes na mesma amostra. À vista disso, é fundamental o desenvolvimento de novos métodos que tenham por finalidade minimizar estes efeitos. Uma alternativa é fazer o uso de técnicas rápidas de extração e separação seguida pelas análises por FT- ICR MS. Desta forma, este estudo teve dois objetivos principais: primeiro, realizar uma extração dos compostos nitrogenados de resíduo de vácuo em suas frações básica e neutra utilizando diferentes métodos e, segundo realizar um fracionamento dos compostos nitrogenados básicos presentes em RV de acordo com a sua massa. Para se alcançar o primeiro objetivo, três métodos foram testados: Extração líquido-líquido (LLE), cromatografia em coluna clássica (LC) e extração em fase sólida (SPE). Os resultados mostraram que o LLE foi a menos seletiva dentre os três procedimentos estudados. SPE e LC apresentaram resultados semelhantes e foi possível separar parcialmente os compostos básicos das espécies neutras. Para alcançar o segundo objetivo, um fracionamento utilizando extração em fase sólida foi empregado na amostra de resíduo de vácuo. Este procedimento permitiu detectar compostos que aparentemente não estavam presentes na amostra não tratada como consequência da redução do efeito de supressão iônica. A ionização por electrospray e por espectrometria de massa de alta resolução (FT-ICR MS) foram utilizados na análise de cada amostra.

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Livres sur le sujet "NanoLC FT MS/MS"

Griffiths, Peter R. GC/FT-IR and GC/FT-IR/MS techniques for routine environmental analysis. Las Vegas, Nev : U.S. Environmental Protection Agency, Research and Development, Environmental Monitoring Systems Laboratory, 1986.

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Griffiths, Peter R. GC/FT-IR and GC/FT-IR/MS techniques for routine environmental analysis. Las Vegas, Nev : U.S. Environmental Protection Agency, Environmental Monitoring Systems Laboratory, 1986.

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Griffiths, Peter R. GC/FT-IR and GC/FT-IR/MS techniques for routine environmental analysis. Las Vegas, Nev : U.S. Environmental Protection Agency, Environmental Monitoring Systems Laboratory, 1986.

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Griffiths, Peter R. GC/FT-IR and GC/FT-IR/MS techniques for routine environmental analysis. Las Vegas, Nev : U.S. Environmental Protection Agency, Environmental Monitoring Systems Laboratory, 1986.

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Griffiths, Peter R. GC/FT-IR and GC/FT-IR/MS techniques for routine environmental analysis. Las Vegas, Nev : U.S. Environmental Protection Agency, Environmental Monitoring Systems Laboratory, 1986.

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Bruce, Asamoto, dir. FT-ICR/MS : Analytical applications of Fourier transform ion cyclotron resonance mass spectrometry. New York, NY : VCH Publishers, 1991.

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Asamoto, Bruce. FT-Icr/MS : Analytical Applications of Fourier Transform Ion Cyclotron Resonance Mass Spectrometry. VCH Publishers, 1991.

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Asamoto, Bruce. Ft-Icr/MS : Analytical Applications of Fourier Transform Ion Cyclotron Resonance Mass Spectrometry. Vch Pub, 1991.

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Chapitres de livres sur le sujet "NanoLC FT MS/MS"

Higashi, Richard M., Teresa W. M. Fan, Pawel K. Lorkiewicz, Hunter N. B. Moseley et Andrew N. Lane. « Stable Isotope-Labeled Tracers for Metabolic Pathway Elucidation by GC-MS and FT-MS ». Dans Methods in Molecular Biology, 147–67. New York, NY : Springer New York, 2014. http://dx.doi.org/10.1007/978-1-4939-1258-2_11.

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Wagner-Rousset, Elsa, Christine Schaeffer-Reiss, Audrey Bednarczyk, Nathalie Corvaïa, Alain Van Dorsselaer et Alain Beck. « NanoLC Chips MS/MS for the Characterization of N-Glycopeptides Generated from Trypsin Digestion of a Monoclonal Antibody ». Dans Methods in Molecular Biology, 81–91. Totowa, NJ : Humana Press, 2013. http://dx.doi.org/10.1007/978-1-62703-327-5_6.

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Bojórquez-Velázquez, Esaú, José M. Elizalde-Contreras, Jesús Alejandro Zamora-Briseño et Eliel Ruiz-May. « Efficient Protein Extraction Protocols for NanoLC-MS/MS Proteomics Analysis of Plant Tissues with High Proteolytic Activity : A Case Study with Pineapple Pulp ». Dans Methods in Molecular Biology, 281–90. New York, NY : Springer US, 2022. http://dx.doi.org/10.1007/978-1-0716-2429-6_17.

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Heck, Michael, Klaus Blaum, R. Burcu Cakirli, Daniel Rodríguez, Lutz Schweikhard, Stefan Stahl et Marta Ubieto-Díaz. « Dipolar and quadrupolar detection using an FT-ICR MS setup at the MPIK Heidelberg ». Dans TCP 2010, 347–55. Dordrecht : Springer Netherlands, 2011. http://dx.doi.org/10.1007/978-94-007-2588-1_36.

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Miyabayashi, Keiko, Yasuhide Naito, Kazuo Tsujimoto et Mikio Miyake. « Characterization of Constituents of Arabian Vacuum Residues by FT-ICR MS Coupled with Various Ionization Techniques ». Dans ACS Symposium Series, 19–37. Washington, DC : American Chemical Society, 2005. http://dx.doi.org/10.1021/bk-2005-0895.ch002.

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Robins, Chad L., Stephen F. Macha, Victor E. Vandell et Patrick A. Limbach. « Laser Desorption/Ionization (LDI)- and Maldi-Fourier Transform Ion Cyclotron Resonance Mass Spectrometric (FT/ICR/MS) Analysis of Hydrocarbon Samples ». Dans Analytical Advances for Hydrocarbon Research, 405–20. Boston, MA : Springer US, 2003. http://dx.doi.org/10.1007/978-1-4419-9212-3_17.

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Van, Phan, et Nguyen Tien. « 2D-NanoLC-ESI-MS/MS for Separation and Identification of Mouse Brain Membrane Proteins ». Dans Chromatography - The Most Versatile Method of Chemical Analysis. InTech, 2012. http://dx.doi.org/10.5772/48376.

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Nikolaev, Eugene N., Yury I. Kostyukevich et Gleb Vladimirov. « Fundamentals and simulations in FT-ICR-MS ». Dans Fundamentals and Applications of Fourier Transform Mass Spectrometry, 89–111. Elsevier, 2019. http://dx.doi.org/10.1016/b978-0-12-814013-0.00004-1.

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Pastor, Salvador J., et Charles L. Wilkins. « Laser Fourier Transform Mass Spectrometry (FT-MS) ». Dans Mass Spectrometry of Polymers, 389–418. CRC Press, 2001. http://dx.doi.org/10.1201/9781420037753-9.

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Pastor, Salvador, et Charles Wilkins. « Laser Fourier Transform Mass Spectrometry (FT- MS) ». Dans Mass Spectrometry of Polymers. CRC Press, 2001. http://dx.doi.org/10.1201/9781420037753.ch9.

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Actes de conférences sur le sujet "NanoLC FT MS/MS"

Ahmed, Zamzam Mohammed, Abrar Mohammed Salem, Jose Ramon, Liu Pei Wu et Benjamin Mowad. « First Successful Pilot Testing of Unconventional Reservoir in North Kuwait from Scratch to Productivity ». Dans Abu Dhabi International Petroleum Exhibition & Conference. SPE, 2021. http://dx.doi.org/10.2118/207394-ms.

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Abstract Jurassic's kerogen shale-carbonate reservoir in North Kuwait is categorized as a source rock exhibiting micro- to Nano Darcy permeability and is Kuwait Oil Company's focus in recent years. Although the challenges are significant (formation creep, fracturing initiation, etc.), the efforts toward producing from unconventional reservoirs and applying experience from both USA and Canada in this field are ongoing. As a step toward development, the gas field development group selected a vertical pilot well to measure the inflow of hydrocarbon from a single fracture while minimizing formation creep (flowing of particulate material and formation into the wellbore that blocks the production). This step was required prior to drilling a long horizontal lateral wells and completing it with multiple hydraulic fractures to confirm commercial production. A comprehensive design process was executed with the full integration of operator and service company competencies to achieve the three main objectives: First, characterize the kerogen rock mechanics which allows selection of the most competent kerogen beds to prevent collapse of the hole during fracturing (creep effect) by conducting scratch, unconfined stress, proppant embedment, and fluid compatibility tests. Then, prepare a suit of strength measurements on full core samples to help in fracturing design and minimize creep effect. The second objective was to design and implement a robust proppant fracturing program that avoids the kerogen concerns after selecting the most competent reservoir unit and suitable proppant type. Third, perform controlled flowback to unload the well and attempt to establish clean inflow unlike previous attempts that failed to either suitably stimulate or prevent solids production (deliver clean inflow). After analyzing the lab test results, choosing the optimal fracturing design, and preparing the vertical well for proppant hydraulic fracturing, the treatment was performed. In December 2019, the hydraulic fracturing treatment with resin-coated bauxite proppant was successfully pumped through 6 ft of perforation interval and followed by a controlled flowback. Resin-coated bauxite proppant was specifically selected to overcome the creep and embedment effects during the fracture closure and flowback. Moreover, a properly designed choke schedule was implemented to balance unloading with a delicate enough drawdown to avoid formation failure. This paper discusses in detail the lab testing, evolution of fracturing design, treatment analysis, and the robust workflow that led to successfully achieving all main objectives, paving the way for long horizontal lateral wells. This unconventional undertaking in Kuwait presents a real challenge. It is a departure from traditional methods, yet it points toward a high upside potential should the appraisal campaign be completed effectively.

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Ahmed, Zamzam Mohammed, Abrar Mohammed Salem, Liu Pei Wu et Benjamin Mowad. « Achieving Productivity and Clean Inflow from an Unconventional Reservoir in North Kuwait ». Dans SPE International Hydraulic Fracturing Technology Conference & Exhibition. SPE, 2022. http://dx.doi.org/10.2118/205253-ms.

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Abstract Jurassic Kerogen shale/carbonate reservoir in North Kuwait provides the same challenges as North American shales in addition to ones not yet comparable to any other analogue reservoir globally. It is the Kerogen's resource density; however, that makes this play so attractive. Like ‘conventional’ unconventional in the US and Canada this kerogen is believed to be a source rock and is on the order of micro-to nano-Darcy permeability. As such, industry learnings show that likely long horizontal laterals with multiple hydraulic fractures will be necessary to make commercial wells. Following this premise, the immediate objective is to establish clean inflow into wellbore as the previous attempts to appraise failed due to "creep" of particulate material and formation flowing into the wellbore. Achieving this milestone will confirm that this formation is capable of solids free inflow and will open a new era in unconventional in Kuwait. Planning for success, the secondary objective is to then upscale to full field development. The main uncertainties lie in both producibility and ‘frac-ability’, and certainly, these challenges are not trivial. A fully integrated testing program was applied to both better understand the rock mechanical properties and to land on an effective frac design. Scratch, unconfined stress, proppant embedment and fluid compatibility tests were conducted on full core samples for geo-mechanics to prepare a suite of strength measurements ahead of frac design and to custom-design the fracture treatment and "controlled" flowback programs to establish inflow from Kerogen without "creep". Unlike developed shale reservoirs, the Jurassic Kerogen tends to become unconsolidated when treated. The pre-frac geomechanics tests will be outlined in this paper with the primary objective of finding the most competent reservoir unit to select the limited perforation interval to frac through so that formation competency can be maintained. Previous attempts failed to maintain a competent rock matrix even only after pumping data-fracs. Acidizing treatments also turn the treated rock volume into sludgy material with no in-situ stability nor ability to deliver "clean inflow". A propped fracturing treatment with resin-coated bauxite was successfully placed in December 2019 in a vertical appraisal well perforated over 6 ft at 12 spf shot density. "Controlled" flowback carried out in January 2020 achieved the strategically critical "clean inflow" with reservoir fluids established to surface. Special proppant technologies provided by an industry leading manufacturer overcame the embedment effects and to control solids flowback. A properly designed choke schedule to balance unloading with a delicate enough drawdown to avoid formation failure was executed. Local oilfields relied on the vast reserves and produced easily from carbonate reservoirs that required only perforating or acid squeezes to easily meet or exceed high production expectations. This unconventional undertaking in Kuwait presents a real challenge as it is a complete departure from the ways of working yet it points towards a very high upside potential should the appraisal campaign can be completed effectively.

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May, Richard, Carlos Perez, Andres Perdoma, Africa Jimenez, John Angel, Nelson Salinas, Efrain Rodriguez, Camilo Angarita et Jose Mierez. « Identifying and Surviving a 1500 ft Long Keyseat @18,000 ft - A Case History and Lessons Learned ». Dans IADC/SPE Drilling Conference and Exhibition. Society of Petroleum Engineers, 2014. http://dx.doi.org/10.2118/168042-ms.

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Bhat, Shankar U., William L. Greiner et Dave Barton. « DeepStar 10,000-ft Water Depth DDCV Study ». Dans Offshore Technology Conference. Offshore Technology Conference, 2003. http://dx.doi.org/10.4043/15102-ms.

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Hammett, D. S. « Drilling Technology Application : 15,000-Ft Water Depth ». Dans IADC/SPE Drilling Conference. Society of Petroleum Engineers, 1986. http://dx.doi.org/10.2118/14726-ms.

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Giam, Choo Seng, T. L. Holliday, M. S. Ahmed, G. E. Reed et G. Zhao. « Pseudomolecular ion formation of explosives in FT-ICR-MS ». Dans Substance Identification Technologies, sous la direction de Geoffrey L. Harding, Richard C. Lanza, Lawrence J. Myers et Peter A. Young. SPIE, 1994. http://dx.doi.org/10.1117/12.171243.

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Gupta, Himanshu, Lyle Finn et John Halkyard. « Spar Riser Alternatives for 10,000 ft Water Depth ». Dans Offshore Technology Conference. Offshore Technology Conference, 2002. http://dx.doi.org/10.4043/14298-ms.

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Norman, Monica, Leon Tveit, Oystein Kalvenes et Jarl Ove Humblen. « The Challenges of Drilling Wells Approaching 40,000 ft. » Dans IADC/SPE Drilling Conference. Society of Petroleum Engineers, 1996. http://dx.doi.org/10.2118/35104-ms.

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Gordy, C. A., J. F. Combes et M. A. Childers. « Case History of a 22,000-ft Deepwater Wildcat ». Dans SPE/IADC Drilling Conference. Society of Petroleum Engineers, 1987. http://dx.doi.org/10.2118/16084-ms.

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Yin, Guoqiang, Salahaldeen Almasmoom, Mohammad Ali et Nawaf Almalki. « Technical Challenges and Strategic Solutions for a New Historical Milestone : The Success of Delivering the First Two 10 K ft Lateral Wells in an Unconventional Drilling Operation ». Dans Middle East Oil, Gas and Geosciences Show. SPE, 2023. http://dx.doi.org/10.2118/213681-ms.

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Abstract To increase the reservoir contact to improve the production, the operator extended the lateral length of the horizontal wells from initial 5 K ft to 6 K ft, then to 7 K ft until 8 K ft. While moving toward a 10 K ft lateral, a bottle neck was encountered and the drilling team had to made all the effort to seek the solution. This paper addressed the main technical challenges to be faced while drilling the first two extended reach 10 K ft lateral wells. Several key strategic solutions corresponding to those challenges were proposed during the planning phase and well implemented in operation. As a result, the first two 10 K ft lateral wells were successfully drilled and completed, establishing a new historical milestone for future operation. In this paper, the inefficient weight transfer while drilling because of the pipe buckling, wellbore instability, the severe losses of the weak formation, failure to build the curve and difficulties for the well placement in lateral, downhole tool directional tool failure due to the high bottom-hole-circulation-temperatures (BHCT) and the risk of unable to run the production casing to bottom were considered as the main challenges to deliver the 10 K ft lateral wells. To overcome these challenges, according to the established best practice and the available tools or devices that have been already tested and prepared, the drilling team performed profound analysis and established the corresponding strategic solutions for each challenge. This paper described the implementation process of those strategies on the first two 10 K ft lateral wells delivered. The experiences obtained were also addressed.

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Rapports d'organisations sur le sujet "NanoLC FT MS/MS"

Noah, M., B. Horsfield, N. Mahlstedt et C. J. Boreham. Maturation characteristics of samples from Wallara-1 well, Canning Basin, as deduced from FT-ICR-MS analysis. Geoscience Australia, 2020. http://dx.doi.org/10.11636/record.2020.058.

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Chunshan Song, P. G. Hatcher, A. K. Saini et K. A. Wenzel. Effects of low-temperature catalytic pretreatments on coal structure and reactivity in liquefaction. Final technical report, Volume 2 - hydrogenative and hydrothermal pretreatments and spectroscopic characterization using pyrolysis-GC-MS, CPMAS {sup 13}C NMR and FT-IR. Office of Scientific and Technical Information (OSTI), janvier 1998. http://dx.doi.org/10.2172/589262.

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Borch, Thomas, Yitzhak Hadar et Tamara Polubesova. Environmental fate of antiepileptic drugs and their metabolites : Biodegradation, complexation, and photodegradation. United States Department of Agriculture, janvier 2012. http://dx.doi.org/10.32747/2012.7597927.bard.

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Many pharmaceutical compounds are active at very low doses, and a portion of them regularly enters municipal sewage systems and wastewater-treatment plants following use, where they often do not fully degrade. Two such compounds, CBZ and LTG, have been detected in wastewater effluents, surface waters, drinking water, and irrigation water, where they pose a risk to the environment and the food supply. These compounds are expected to interact with organic matter in the environment, but little is known about the effect of such interactions on their environmental fate and transport. The original objectives of our research, as defined in the approved proposal, were to: Determine the rates, mechanisms and products of photodegradation of LTG, CBZ and selected metabolites in waters exposed to near UV light, and the influence of DOM type and binding processes on photodegradation. Determine the potential and pathways for biodegradation of LTG, CBZ and selected metabolites using a white rot fungus (Pleurotusostreatus) and ADP, and reveal the effect of DOM complexation on these processes. Reveal the major mechanisms of binding of LTG, CBZ and selected metabolites to DOM and soil in the presence of DOM, and evaluate the effect of this binding on their photodegradation and/or biodegradation. We determined that LTG undergoes relatively slow photodegradation when exposed to UV light, and that pH affects each of LTG’s ability to absorb UV light, the efficiency of the resulting reaction, and the identities of LTG’sphotoproducts (t½ = 230 to 500 h during summer at latitude 40 °N). We observed that LTG’sphotodegradation is enhanced in the presence of DOM, and hypothesized that LTG undergoes direct reactions with DOM components through nucleophilic substitution reactions. In combination, these data suggest that LTG’s fate and transport in surface waters are controlled by environmental conditions that vary with time and location, potentially affecting the environment and irrigation waters. We determined that P. ostreatusgrows faster in a rich liquid medium (glucose peptone) than on a natural lignocellulosic substrate (cotton stalks) under SSF conditions, but that the overall CBZ removal rate was similar in both media. Different and more varied transformation products formed in the solid state culture, and we hypothesized that CBZ degradation would proceed further when P. ostreatusand the ᵉⁿᶻʸᵐᵃᵗⁱᶜ ᵖʳᵒᶠⁱˡᵉ ʷᵉʳᵉ ᵗᵘⁿᵉᵈ ᵗᵒ ˡⁱᵍⁿⁱⁿ ᵈᵉᵍʳᵃᵈᵃᵗⁱᵒⁿ. ᵂᵉ ᵒᵇˢᵉʳᵛᵉᵈ ¹⁴C⁻Cᴼ2 ʳᵉˡᵉᵃˢᵉ ʷʰᵉⁿ ¹⁴C⁻ᶜᵃʳᵇᵒⁿʸˡ⁻ labeled CBZ was used as the substrate in the solid state culture (17.4% of the initial radioactivity after 63 days of incubation), but could not conclude that mineralization had occurred. In comparison, we determined that LTG does not degrade in agricultural soils irrigated with treated wastewater, but that P. ostreatusremoves up to 70% of LTG in a glucose peptone medium. We detected various metabolites, including N-oxides and glycosides, but are still working to determine the degradation pathway. In combination, these data suggest that P. ostreatuscould be an innovative and effective tool for CBZ and LTG remediation in the environment and in wastewater used for irrigation. In batch experiments, we determined that the sorption of LTG, CBZ and selected metabolites to agricultural soils was governed mainly by SOM levels. In lysimeter experiments, we also observed LTG and CBZ accumulation in top soil layers enriched with organic matter. However, we detected CBZ and one of its metabolites in rain-fed wheat previously irrigated with treated wastewater, suggesting that their sorption was reversible, and indicating the potential for plant uptake and leaching. Finally, we used macroscale analyses (including adsorption/desorption trials and resin-based separations) with molecular- level characterization by FT-ICR MS to demonstrate the adsorptive fractionation of DOM from composted biosolids by mineral soil. This suggests that changes in soil and organic matter types will influence the extent of LTG and CBZ sorption to agricultural soils, as well as the potential for plant uptake and leaching.

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