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Articles de revues sur le sujet « Protein analogue »

Auteur : Grafiati

Publié le 9 mars 2023

Mis à jour le 31 juillet 2025

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1

Shi, Yizhe. "Methodologies of meat analogue production and nutritional perspectives: a review." Theoretical and Natural Science 65, no. 1 (2024): 10–23. https://doi.org/10.54254/2753-8818/2024.la17931.

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With increasing global population and environmental concerns, the development of meat analogues, including plant-based proteins and cultivated meat, has become a solution for elevating global protein production and addressing sustainability issues. North America and Europe lead the meat analogue market, driven by elevated consumer awareness of health and environmental concerns. Under the influence of western diet, the Asian market is also poised for growth. While cultivated meat industry remains immature, advancements in production and cost reductions are expected to boost its market share ove

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Micallef, Jacob V., Margaret M. Hayes, Abdul Latif, Rukhsana Ahsan, and Saulat B. Sufi. "Serum Binding of Steroid Tracers and its Possible Effects on Direct Steroid Immunoassay." Annals of Clinical Biochemistry: International Journal of Laboratory Medicine 32, no. 6 (1995): 566–74. http://dx.doi.org/10.1177/000456329503200609.

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We studied the serum protein binding of 3H-labelled progesterone, oestradiol and testosterone, and five 125I-labelled analogues of these steroids. All tracers investigated appeared to be bound by proteins in every serum sample tested. The addition of blocking agents caused a substantial reduction in serum protein binding of 3H-labelled steroids, but had relatively little effect on the binding of analogue steroid tracers. Use of analogue steroid tracers in conventional direct immunoassays for oestradiol and progesterone produced anomalous results for some patient samples when compared to extrac

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Volonté, C., A. H. Ross, and L. A. Greene. "Association of a purine-analogue-sensitive protein kinase activity with p75 nerve growth factor receptors." Molecular Biology of the Cell 4, no. 1 (1993): 71–78. http://dx.doi.org/10.1091/mbc.4.1.71.

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Purine analogues are protein kinase inhibitors, and they block with varying potency and specificity certain of the biological actions of nerve growth factor (NGF). The analogue 6-thioguanine (6-TG) has been shown to inhibit with high specificity protein kinase N (PKN), a serine/threonine protein kinase activated by NGF in several cellular systems. In the present work, immunoprecipitates of p75 NGF receptors from PC12 cells (+/-NGF treatment) were assayed for protein kinase activity using the substrate myelin basic protein under phosphorylating conditions optimal for PKN and in the presence or

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Wibowo, K. F., Y. R. Swasti, and F. S. Pranata. "Low fat and high protein meat analogue of cowpea (Vigna unguiculata) with stabilizer cocoa pod husk extract (Theobroma cacao L.)." Food Research 7, no. 5 (2023): 1–11. http://dx.doi.org/10.26656/fr.2017.7(5).789.

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Meat analogue is a plant-based protein product with nutritional and sensory qualities similar to conventional meat. Meat analogue is usually made from wheat flour. People with gluten intolerance can not consume wheat flour. Gluten-free meat analogue is appropriate for such people. In this research, gluten-free meat analogue was made from cowpea flour and cocoa pod husk extract, in three different formulations. Cocoa pod husk extract serves as stabilizer and sticking agent. The formulation was based on the ratio of the main ingredients. The ratios were 98.5:1.5 (A), 99.0:1.0 (B), and 99.5:0.5 (

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Wang, Tianxiao, Lovedeep Kaur, Yasufumi Furuhata, Hiroaki Aoyama, and Jaspreet Singh. "3D Printing of Textured Soft Hybrid Meat Analogues." Foods 11, no. 3 (2022): 478. http://dx.doi.org/10.3390/foods11030478.

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Meat analogue is a food product mainly made of plant proteins. It is considered to be a sustainable food and has gained a lot of interest in recent years. Hybrid meat is a next generation meat analogue prepared by the co-processing of both plant and animal protein ingredients at different ratios and is considered to be nutritionally superior to the currently available plant-only meat analogues. Three-dimensional (3D) printing technology is becoming increasingly popular in food processing. Three-dimensional food printing involves the modification of food structures, which leads to the creation

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Vila-Clarà, Gil, Anna Vila-Martí, Laia Vergés-Canet, and Miriam Torres-Moreno. "Exploring the Role and Functionality of Ingredients in Plant-Based Meat Analogue Burgers: A Comprehensive Review." Foods 13, no. 8 (2024): 1258. http://dx.doi.org/10.3390/foods13081258.

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The development of plant-based meat analogues has become a significant challenge for the food industry in recent years due to the increasing demand for sustainable and healthier proteins in the context of a global protein transition. Plant-based meat analogues imitate the visual, textural, and chemical properties of traditional meat products and are required to closely resemble meat to appeal to consumers. In addition, consumers demand natural, clean-label, and nutritional, and healthy products. To address these challenges, the food industry must develop highly healthy, nutritious, and E-numbe

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Sobhonslidsuk, Abhasnee, Jirachaya Wanichanuwat, Pawin Numthavaj, et al. "Nucleotide Analogue-Related Proximal Renal Tubular Dysfunction during Long-Term Treatment of Chronic Hepatitis B: A Cross-Sectional Study." Gastroenterology Research and Practice 2016 (2016): 1–7. http://dx.doi.org/10.1155/2016/2952635.

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Background. There have been few reports of nucleotide analogue-related renal tubular dysfunction (RTD) in CHB patients. We assessed the prevalence and presentation of nucleotide analogue-related proximal RTD.Methods. A cross-sectional study was performed in CHB patients taking nucleotide analogues. Inclusion criteria were patients who were on adefovir or tenofovir as mono- or add-on therapy with lamivudine (LAM) >1 year. Serum and urine were collected. Fractional excretion of phosphate (FEPO4), uric acid (FEUA), and potassium was calculated. Renal losses were defined based on the criteria:

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Andika, Ari, Feri Kusnandar, and Slamet Budijanto. "KARAKTERISTIK FISIKOKIMIA DAN SENSORI BERAS ANALOG MULTIGRAIN BERPROTEIN TINGGI." Jurnal Teknologi dan Industri Pangan 32, no. 1 (2021): 60–71. http://dx.doi.org/10.6066/jtip.2021.32.1.60.

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Several grains (green bean, red bean, soybean, corn, nuts, sesame, and millets) were processed to yield a high protein analogue rice. Red beans and green beans were soaked in water for six hours while soybean was boiled for 10 minutes and then peeled. Nuts were dried at 70°C, ground, and sieved to pass 80 mesh. All grains were ground into powder except for sesame which was in whole seed. Four formulas of rice analogues were produced at a different level of millet (0-15%), corn (35-50%) with fixed level of red beans (10%), soybeans (25%), green beans (10%), sesame (3%), and glycerol monostearat

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Wingfield, P. T., P. Graber, G. Buell, K. Rose, M. G. Simona, and B. D. Burleigh. "Preparation and characterization of bovine growth hormones produced in recombinant Escherichia coli." Biochemical Journal 243, no. 3 (1987): 829–39. http://dx.doi.org/10.1042/bj2430829.

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Two analogues of bovine growth hormone (BGH) have been produced in Escherichia coli by recombinant DNA techniques. In analogue Delta-1, the N-terminal alanine residue of the full-length bovine sequence is replaced by methionine. In analogue Delta-9, which is expressed at much higher levels than is Delta-1, the full-length bovine sequence is truncated at the N-terminus by eight residues and there is a serine-for-glycine substitution in the first position of the truncated protein. Both analogues, which were characterized by isoelectric focusing (i.e.f.), polyacrylamide-gel electrophoresis in the

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WANG, Yanlin, Amy HACKER, Tracy MURRAY-STEWART, Jennifer G. FLEISCHER, Patrick M. WOSTER, and Robert A. CASERO. "Induction of human spermine oxidase SMO(PAOh1) is regulated at the levels of new mRNA synthesis, mRNA stabilization and newly synthesized protein." Biochemical Journal 386, no. 3 (2005): 543–47. http://dx.doi.org/10.1042/bj20041084.

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The oxidation of polyamines induced by antitumour polyamine analogues has been associated with tumour response to specific agents. The human spermine oxidase, SMO(PAOh1), is one enzyme that may play a direct role in the cellular response to the antitumour polyamine analogues. In the present study, the induction of SMO(PAOh1) enzyme activity by CPENSpm [N1-ethyl-N11-(cyclopropyl)methyl-4,8,diazaundecane] is demonstrated to be a result of newly synthesized mRNA and protein. Inhibition of new RNA synthesis by actinomycin D inhibits both the appearance of SMO(PAOh1) mRNA and enzyme activity. Simil

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Snir, Barak, Ayelet Fishman, and Jovana Glusac. "Chickpea-Based Milk Analogue Stabilized by Transglutaminase." Foods 14, no. 3 (2025): 514. https://doi.org/10.3390/foods14030514.

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Plant-based milk substitutes are becoming increasingly popular in the food industry. Among different plant proteins, chickpea proteins (CP) offer unique qualities as good functional and nutritional properties, followed by pleasant taste. This study examines the ability of the production of o/w emulsions resembling milk analogue (3% w/w chickpea protein, 3% w/w canola oil) by using chickpea protein isolate with/without the enzyme transglutaminase (TG) (50 U/g of protein). As a reference material, commercial soymilk was used. The emulsions were characterized by particle size distribution, zeta p

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Frimayanti, Neni, Rahma Dona, and Tabah Solihin. "Molecular Docking Study of Chalcone Analogue Compounds with Hydroxy and Methoxy Subtituents as Bcl-2 Inhibitors." CHEMPUBLISH JOURNAL 7, no. 1 (2023): 31–41. http://dx.doi.org/10.22437/chp.v7i1.17487.

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Molecular docking study of 6 chalcone analogues with protein target from the crystallographic structure modeling of Bcl-2 protein with PDB code 2W3L was carried out using computational media using the Molecular Operating Environment (MOE) program. The aim of this study is to determine the potentiality of the 6 chalcone analogue compounds as Bcl-2 inhibitors using molecular docking studies. In this study, venetoclax was used as positive control. Based on docking results, binding free energy was used as information to know which wheather chalcone analogue compounds are active or not as Bcl-2 inh

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Aini, N., B. Sustriawan, V. Prihananto, J. Sumarmono, R. N. Ramadan, and D. Romadhon. "Formulation of low-fat cheese analogue from sweet corn extract using papain and lime extract as coagulant." Food Research 4, no. 4 (2020): 1071–81. http://dx.doi.org/10.26656/fr.2017.4(4).395.

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Cheese is not only created using cow's milk and can also be made from a mixture of vegetable extracts, including corn extract. Cheese from corn extract has the advantages of low-fat and high-carotene. Notably, papain can be used as a coagulant in the production of cheese analogue, while maltodextrin functions to increase volume and total solids for greater yield. The objectives of the present study was 1) to optimize the formula composition between lime extract, papain, and maltodextrin to create a cheese analogue from sweet corn extract with high yield and protein as well as good sensory prop

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Guay-Woodford, L. M., O. Platt, and H. W. Harris. "Toad urinary bladder epithelial cells contain an analogue of cytoskeletal protein 4.1." American Journal of Physiology-Cell Physiology 260, no. 6 (1991): C1308—C1314. http://dx.doi.org/10.1152/ajpcell.1991.260.6.c1308.

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Epithelial cell polarity and vectorial transport require cytoskeletal proteins that maintain local cell membrane structure and mediate cytoplasmic vesicle movement. The cytoskeleton of leaky epithelia, such as the intestinal mucosa and renal proximal tubule cells, has been extensively studied. However, cytoskeletal studies in tight epithelia such as the mammalian collecting duct and toad urinary bladder generally have been confined to ultrastructural investigation. Recent research in nonepithelial cell types has identified an interesting family of cytoskeletal proteins. Present in multiple cel

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Girija, J., S. Kamalasundari, G. Hemalatha, and T. Umamaheswari. "Production Methodologies of Meat Analogues: A Review." Journal of Agricultural Engineering 58, no. 02 (2021): 137–48. http://dx.doi.org/10.52151/jae2021581.1741.

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Meat is a non-vegetarian food and is considered as a good source of quality nutrients. Though meat protein provide the required content of good quality protein for the body, they are also associated with higher cholesterol and fat content, which prove to be a leading cause of serious health issues. This became the primary reason for increase in a shift in demands for plant-based protein source foods. The other reason is environmental impact of animal derived products. Meat analogues are plant-based good quality protein source of food that tastes like meat protein, and texture resemble that of

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Ferawati, Ferawati, Izalin Zahari, Malin Barman, et al. "High-Moisture Meat Analogues Produced from Yellow Pea and Faba Bean Protein Isolates/Concentrate: Effect of Raw Material Composition and Extrusion Parameters on Texture Properties." Foods 10, no. 4 (2021): 843. http://dx.doi.org/10.3390/foods10040843.

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Yellow pea and faba bean are potential candidates to replace soybean-based ingredients due to their suitability for cultivation in the northern hemisphere, non-genetically modified organisms cultivation practice and low risk of allergenicity. This study examined the functionality of local yellow pea and faba bean protein isolates/concentrate as meat analogue products. The most critical factors affecting the texture properties of meat analogue were also determined. Extrusion was used to produce high-moisture meat analogues (HMMAs) from yellow pea and faba bean protein isolates/concentrates and

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Yin, Feifei, Manli Wang, Ying Tan, et al. "A Functional F Analogue of Autographa californica Nucleopolyhedrovirus GP64 from the Agrotis segetum Granulovirus." Journal of Virology 82, no. 17 (2008): 8922–26. http://dx.doi.org/10.1128/jvi.00493-08.

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ABSTRACT The envelope fusion protein F of Plutella xylostella granulovirus is a computational analogue of the GP64 envelope fusion protein of Autographa californica nucleopolyhedrovirus (AcMNPV). Granulovirus (GV) F proteins were thought to be unable to functionally replace GP64 in the AcMNPV pseudotyping system. In the present study the F protein of Agrotis segetum GV (AgseGV) was identified experimentally as the first functional GP64 analogue from GVs. AgseF can rescue virion propagation and infectivity of gp64-null AcMNPV. The AgseF-pseudotyped AcMNPV also induced syncytium formation as a c

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Ülger, Taha Gökmen, Esra Tunçer, Esra Sarıca, Eda Nur Kutlubağ, Gülsüm Altuğ, and Miray Bahadır. "Investigation of nutritional profile, sensory properties and antioxidant potential of legume-based yoghurt analogues." Food and Health 11, no. 3 (2025): 258–67. https://doi.org/10.3153/fh25022.

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This study investigates the functional food potential of legume-based yoghurt analogues derived from chickpeas, lupins, and beans by analysing their sensory, macronutrient, antioxidant, and physicochemical properties, comparing them with conventional dairy yoghurt. Sensory evaluation showed that dairy yoghurt received the highest scores, while lupin-based yoghurt analogue had the lowest. Chickpea- and bean-based yoghurt analogues were better accepted than lupin-based products in terms of sensory attributes (p<0.05). Bean-based yoghurt analogue had a significantly higher protein content (6.3

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Xu, Yunjian, Frank Schwede, Hans Wienk, Anders Tengholm, and Holger Rehmann. "A Membrane Permeable Prodrug of S223 for Selective Epac2 Activation in Living Cells." Cells 8, no. 12 (2019): 1589. http://dx.doi.org/10.3390/cells8121589.

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Signalling by cyclic adenosine monophosphate (cAMP) occurs via various effector proteins, notably protein kinase A and the guanine nucleotide exchange factors Epac1 and Epac2. These proteins are activated by cAMP binding to conserved cyclic nucleotide binding domains. The specific roles of the effector proteins in various processes in different types of cells are still not well defined, but investigations have been facilitated by the development of cyclic nucleotide analogues with distinct selectivity profiles towards a single effector protein. A remaining challenge in the development of such

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Handayani, Noer Abyor, Siswo Sumardiono, Aprilina Purbasari, Alfan Fatir Fatikah, and Imam Muda Alhakim. "Physicochemical Properties of Sago- and Corn Flour-Based Rice Analogues Fortified with Black-Eyed Bean Flour and Skimmed Milk Powder." Food Technology and Biotechnology 62, no. 4 (2024): 501–11. https://doi.org/10.17113/ftb.62.04.24.8357.

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Research background. With the increasing consumption of food commodities, particularly rice, and the substantial volume of food imports in Indonesia, there is an increasing need to explore alternative food sources. Rice analogues emerge as a potential substitute for traditional rice, serving as a viable staple food option. The aim of this study is to investigate the effect of the composition of raw material, namely sago and corn flour, on the physicochemical properties and consumer acceptance of rice analogues. Experimental approach. The rice analogues were produced using the hot extrusion met

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Maulida, Arlin, Wahyu Ramadhan, and Joko Santoso. "The Development of Hybrid Food as Meat Analog Strips Based on Seaweed Ulva ohnoi and Surimi." BIO Web of Conferences 147 (2024): 01021. https://doi.org/10.1051/bioconf/202414701021.

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Meat analogues have been gaining popularity as a sustainable and health-conscious alternative to traditional meat products, driven by increasing consumer awareness of environmental benefits. However, there remains a gap in the market for meat analogues that not only mimic meat texture but also offer enhanced nutritional benefits, particularly in terms of fiber content. Meat analogue strips from Ulva ohnoi and surimi underwent proximate, fiber, and texture profile analysis. The aim of the study was to determine the best formula between the ratio of Ulva ohnoi and surimi inclusion in meat analog

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Lindriati, Triana, Ahmad Nafi, and Zelika Gita Sari. "Optimasi Pembuatan Daging Tiruan Umbi Porang (Amorphophallus oncophyllus) dan Isolat Protein Kedelai dengan Metode RSM (Response Surface Methodology)." Jurnal Teknologi dan Industri Pertanian Indonesia 11, no. 2 (2020): 75–83. http://dx.doi.org/10.17969/jtipi.v11i2.12798.

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Meat analogue is a form of product which has originated from vegetal component which has a lot of tissue and could replace animal meat. The forming of meat analogue is using the extrusion technique. Primary ingredient which used is isolate soy protein and water, but also it is able to be added by other carbohydrates source such as porang bulb (Amorphophallus oncophyllus) wherewith increase the functional character over the meat analogue. This research is aimed for identify the optimum condition of proper treatment between the water additional formulation, isolate soy protein, porang bulb, ther

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Yang, Xiaochen, Hui Miao, Ruotong Xiao, et al. "Diverse protein manipulations with genetically encoded glutamic acid benzyl ester." Chemical Science 12, no. 28 (2021): 9778–85. http://dx.doi.org/10.1039/d1sc01882e.

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Yuan, Xinyue, Wei Jiang, Dianwei Zhang, Huilin Liu, and Baoguo Sun. "Textural, Sensory and Volatile Compounds Analyses in Formulations of Sausages Analogue Elaborated with Edible Mushrooms and Soy Protein Isolate as Meat Substitute." Foods 11, no. 1 (2021): 52. http://dx.doi.org/10.3390/foods11010052.

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In this study, edible mushroom and soybean protein isolate (SPI) were used to prepare a fibrous meat analogue using thermos-extrusion and the extruded mushroom-based meat analogue as meat replacer was further developed with different formulations in fabricating sausage analogues. The effect of water content (35%, 70% and 100%), three types of edible mushroom (Lentinus edodes, Pleurotus ostreatus, Coprinus comatus and a mixture of equal proportions) and their amounts (from 15% to 100%) on the physicochemical and structural profiles were studied. The results showed that the extruded mushroom-bas

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Deinychenko, Grygorii, Inna Zolotukhina, Viktoriia Skrynnik, Liudmyla Deinychenko, and Tamara Kravchenko. "BIOLOGICAL VALUE OF PROTEIN OF CULINARY PRODUCTS BASED ON MILK-PROTEIN CONCENTRATE." EUREKA: Life Sciences 3 (May 31, 2020): 31–37. http://dx.doi.org/10.21303/2504-5695.2020.001287.

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The aim of this work is to determine the usage effectiveness of milk-protein concentrates as an analogue of cottage cheese at culinary products manufacturing. For attaining the set aim, we determined the biological value of protein in products, made using a milk-protein concentrate, comparing to traditional culinary products of fatless cottage cheese. The research object was chosen as a milk-protein concentrate of buttermilk, obtained by the method of thermo-acid coagulation. Puree of cranberries was used as a coagulant. Classic recipes of different groups of culinary products, based on cottag

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Grygorii, Deinychenko, Zolotukhina Inna, Skrynnik Viktoriia, Deinychenko Liudmyla, and Kravchenko Tamara. "BIOLOGICAL VALUE OF PROTEIN OF CULINARY PRODUCTS BASED ON MILK-PROTEIN CONCENTRATE." EUREKA: Life Sciences, no. 3 (May 31, 2020): 31–37. https://doi.org/10.21303/2504-5695.2020.001287.

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The aim of this work is to determine the usage effectiveness of milk-protein concentrates as an analogue of cottage cheese at culinary products manufacturing. For attaining the set aim, we determined the biological value of protein in products, made using a milk-protein concentrate, comparing to traditional culinary products of fatless cottage cheese. The research object was chosen as a milk-protein concentrate of buttermilk, obtained by the method of thermo-acid coagulation. Puree of cranberries was used as a coagulant. Classic recipes of different groups of culinary products, based on cottag

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Banovic, Marija, and Kolbrún Sveinsdóttir. "Importance of being analogue: Female attitudes towards meat analogue containing rapeseed protein." Food Control 123 (May 2021): 107833. http://dx.doi.org/10.1016/j.foodcont.2020.107833.

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Li, Feng, Wipaporn Khanom, Xia Sun, et al. "Andrographolide and Its 14-Aryloxy Analogues Inhibit Zika and Dengue Virus Infection." Molecules 25, no. 21 (2020): 5037. http://dx.doi.org/10.3390/molecules25215037.

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Andrographolide is a labdene diterpenoid with potential applications against a number of viruses, including the mosquito-transmitted dengue virus (DENV). In this study, we evaluated the anti-viral activity of three 14-aryloxy analogues (ZAD-1 to ZAD-3) of andrographolide against Zika virus (ZIKV) and DENV. Interestingly, one analogue, ZAD-1, showed better activity against both ZIKV and DENV than the parental andrographolide. A two-dimension (2D) proteomic analysis of human A549 cells treated with ZAD-1 compared to cells treated with andrographolide identified four differentially expressed prot

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Georgatos, S. D., I. Maroulakou, and G. Blobel. "Lamin A, lamin B, and lamin B receptor analogues in yeast." Journal of Cell Biology 108, no. 6 (1989): 2069–82. http://dx.doi.org/10.1083/jcb.108.6.2069.

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Previous studies have shown that turkey erythrocyte lamin B is anchored to the nuclear envelope via a 58-kD integral membrane protein termed p58 or lamin B receptor (Worman H. J., J. Yuan, G. Blobel, and S. D. Georgatos. 1988. Proc. Natl. Acad. Sci. USA. 85:8531-8534). We now identify a p58 analogue in the yeast Saccharomyces cerevisiae. Turkey erythrocyte lamin B binds to yeast urea-extracted nuclear envelopes with high affinity, associating predominantly with a 58-kD polypeptide. This yeast polypeptide is recognized by polyclonal antibodies against turkey p58, partitions entirely with the nu

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Gong, J. H., and I. Clark-Lewis. "Antagonists of monocyte chemoattractant protein 1 identified by modification of functionally critical NH2-terminal residues." Journal of Experimental Medicine 181, no. 2 (1995): 631–40. http://dx.doi.org/10.1084/jem.181.2.631.

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Monocyte chemoattractant protein (MCP)-1 analogues were designed to determine the role of the NH2-terminal region in structure and function. The NH2-terminal residue was important for function and receptor binding, as it could not be deleted or extended. However the NH2-terminal pyroglutamate residue of the wild type was not essential as it could be replaced by several other noncyclic amino acids without loss of activity. Residues 7-10 were essential for receptor desensitization, but were not sufficient for function, and the integrity of residues 1-6 were required for functional activity. A pe

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ZEGERS, M. P. Mirjam, Jan Willem KOK, and Dick HOEKSTRA. "Use of photoactivatable sphingolipid analogues to monitor lipid transport in mammalian cells." Biochemical Journal 328, no. 2 (1997): 489–98. http://dx.doi.org/10.1042/bj3280489.

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Photoactivatable derivatives of ceramide, glucosylceramide (GlcCer) and sphingomyelin {3-(p-azido-m-[125I]iodophenyl)propionylceramide, 3-(p-azido-m-[125I]iodophenyl)propionyl-GlcCer and 3-(p-azido-m-[125I]iodophenyl)propionylsphingomyelin} were synthesized in an attempt to identify compartment-specific proteins involved in sphingolipid sorting or metabolism. In HT29 and BHK cells the ceramide analogue entered the cell by monomeric diffusion, as evidenced by the probe's efficient internalization at low temperature (4 °C). In contrast, the photoactivatable GlcCer was internalized only at elevat

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Ubbink, Job, and Belal J. Muhialdin. "Protein physical state in meat analogue processing." Current Opinion in Food Science 45 (June 2022): 100822. http://dx.doi.org/10.1016/j.cofs.2022.100822.

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Fekner, Tomasz, Xin Li, Marianne M Lee, and Michael K Chan. "A Pyrrolysine Analogue for Protein Click Chemistry." Angewandte Chemie International Edition 48, no. 9 (2009): 1633–35. http://dx.doi.org/10.1002/anie.200805420.

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Fekner, Tomasz, Xin Li, Marianne M Lee, and Michael K Chan. "A Pyrrolysine Analogue for Protein Click Chemistry." Angewandte Chemie 121, no. 9 (2009): 1661–63. http://dx.doi.org/10.1002/ange.200805420.

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Samson, M. F., and D. E. Smilek. "Reversal of acute experimental autoimmune encephalomyelitis and prevention of relapses by treatment with a myelin basic protein peptide analogue modified to form long-lived peptide-MHC complexes." Journal of Immunology 155, no. 5 (1995): 2737–46. http://dx.doi.org/10.4049/jimmunol.155.5.2737.

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Abstract Experimental autoimmune encephalomyelitis (EAE) is an autoimmune disease induced by immunization with myelin basic protein (MBP), proteolipid protein, or encephalitogenic peptides from these myelin components. EAE resembles basic protein multiple sclerosis in some of its clinical and histologic features, and serves as an experimental model for this and other autoimmune diseases. In this study, we examine i.v. peptide therapy of EAE in detail, and show that repeated i.v. injections of MBP peptides effectively treat EAE in (PLJxSJL)F1 mice. In this study, administration of the immunodom

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Redmon, J. B., T. W. Gettys, V. S. Sheorain, J. D. Corbin, and I. L. Taylor. "Failure of insulin to antagonize cAMP-mediated glycogenolysis in rat ventricular cardiomyocytes." American Journal of Physiology-Endocrinology and Metabolism 258, no. 5 (1990): E871—E877. http://dx.doi.org/10.1152/ajpendo.1990.258.5.e871.

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Isolated rat ventricular cardiomyocytes were used to study the effects of insulin on glycogen metabolism in cells treated with various agents that activate adenosine 3',5'-cyclic monophosphate (cAMP)-dependent protein kinase. Incubation of myocytes with isoproterenol produced a rapid concentration-dependent increase in cAMP concentration, cAMP-dependent protein kinase activity, and phosphorylase activity and a simultaneous decrease in the glycogen synthase activity ratio. Various cAMP analogues also produced a concentration-dependent increase in phosphorylase activity and a decline in the glyc

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Krawczyk, Anna, Juana Fernández-López, and Anna Zimoch-Korzycka. "Insect Protein as a Component of Meat Analogue Burger." Foods 13, no. 12 (2024): 1806. http://dx.doi.org/10.3390/foods13121806.

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Researchers are exploring solutions to meet the growing demand for protein due to the expected increase in global population by 2050. Interest in alternative protein sources like insects has risen, driven by concerns about environmental impact and the need for sustainable food production. This study aimed to develop and evaluate the physicochemical properties of soy-protein-based burgers enriched with insect protein from Alphitobius diaperinus. Three formulations were developed: a control (B0) and burgers with 5% (B5) and 10% (B10) insect protein—Whole Buffalo Powder (WBP). The results showed

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Matozaki, T., J. Martinez, and J. A. Williams. "A new CCK analogue differentiates two functionally distinct CCK receptors in rat and mouse pancreatic acini." American Journal of Physiology-Gastrointestinal and Liver Physiology 257, no. 4 (1989): G594—G600. http://dx.doi.org/10.1152/ajpgi.1989.257.4.g594.

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Analysis of the competitive inhibition of 125I-labeled cholecystokinin octapeptide (CCK-8) binding to isolated rat or mouse pancreatic acini showed that in both species CCK-8 interacts with two different affinity sites. A newly synthesized CCK analogue modified at the COOH-terminal phenylalanine residue totally inhibited 125I-CCK binding. This interaction occurred with sites of a single affinity in rat acini but with two different affinity sites in mouse acini. When acini were incubated with increasing concentrations of CCK-8, a biphasic stimulation of amylase release was observed. By use of r

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Pennells, Jordan, Louise Trigona, Hetvi Patel, and Danyang Ying. "Ingredient Functionality of Soy, Chickpea, and Pea Protein before and after Dry Heat Pretreatment and Low Moisture Extrusion." Foods 13, no. 14 (2024): 2168. http://dx.doi.org/10.3390/foods13142168.

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This study investigates the impact of dry heat pretreatment on the functionality of soy, chickpea, and pea protein ingredients for use in texturized vegetable protein (TVP) production via low moisture extrusion. The protein powders were heat-treated at temperatures ranging from 80 °C to 160 °C to modulate the extent of protein denaturation and assess their effects on RVA pasting behavior, water absorption capacity (WAC), and color attributes. The results indicate that the pretreatment temperature significantly influenced the proteins’ functional properties, with an optimal temperature of 120 °

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Fawcett, Janet, Frederick G. Hamel, Robert G. Bennett, Zoltan Vajo, and William C. Duckworth. "Insulin and Analogue Effects on Protein Degradation in Different Cell Types." Journal of Biological Chemistry 276, no. 15 (2000): 11552–58. http://dx.doi.org/10.1074/jbc.m007988200.

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In adult animals, the major effect of insulin on protein turnover is inhibition of protein degradation. Cellular protein degradation is under the control of multiple systems, including lysosomes, proteasomes, calpains, and giant protease. Insulin has been shown to alter proteasome activityin vitroandin vivo. We examined the inhibition of protein degradation by insulin and insulin analogues (LysB28,ProB29-insulin (LysPro), AspB10-insulin (B10), and GluB4,GlnB16,PheB17-insulin (EQF)) in H4, HepG2, and L6 cells. These effects were compared with receptor binding. Protein degradation was examined b

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Milligan, G., I. Mullaney, C. G. Unson, L. Marshall, A. M. Spiegel та H. McArdle. "GTP analogues promote release of the α subunit of the guanine nucleotide binding protein, Gi2, from membranes of rat glioma C6 BU1 cells". Biochemical Journal 254, № 2 (1988): 391–96. http://dx.doi.org/10.1042/bj2540391.

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The major pertussis-toxin-sensitive guanine nucleotide-binding protein of rat glioma C6 BU1 cells corresponded immunologically to Gi2. Antibodies which recognize the alpha subunit of this protein indicated that it has an apparent molecular mass of 40 kDa and a pI of 5.7. Incubation of membranes of these cells with guanosine 5′-[beta gamma-imido]triphosphate, or other analogues of GTP, caused release of this polypeptide from the membrane in a time-dependent manner. Analogues of GDP or of ATP did not mimic this effect. The GTP analogues similarly caused release of the alpha subunit of Gi2 from m

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Kyriakopoulou, Konstantina, Julia K. Keppler, and Atze Jan van der Goot. "Functionality of Ingredients and Additives in Plant-Based Meat Analogues." Foods 10, no. 3 (2021): 600. http://dx.doi.org/10.3390/foods10030600.

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Meat analogue research and development focuses on the production of sustainable products that recreate conventional meat in its physical sensations (texture, appearance, taste, etc.) and nutritional aspects. Minced products, like burger patties and nuggets, muscle-type products, like chicken or steak-like cuts, and emulsion products, like Frankfurter and Mortadella type sausages, are the major categories of meat analogues. In this review, we discuss key ingredients for the production of these novel products, with special focus on protein sources, and underline the importance of ingredient func

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DIETRICH, Alexander, Alexander SCHEER, Daria ILLENBERGER, Yoel KLOOG, Yoav I. HENIS та Peter GIERSCHIK. "Studies on G-protein α·βγ heterotrimer formation reveal a putative S-prenyl-binding site in the α subunit". Biochemical Journal 376, № 2 (2003): 449–56. http://dx.doi.org/10.1042/bj20030578.

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The α and βγ subunits of heterotrimeric G-proteins contain specific lipid modifications, which are required for their biological function. However, the relevance of these modifications to the interactions within the heterotrimeric G-protein is not fully understood. In order to explore the role of the S-prenyl moiety of the isoprenylated βγ dimer of retinal transducin, βγt, in the formation of the heterotrimeric complex with the corresponding N-acylated α subunit, αt, we employed purified fully processed subunits, which are soluble in aqueous solutions without detergents. Pertussis-toxin-mediat

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Kotera, T., and P. D. Brown. "Cl- current activation in choroid plexus epithelial cells involves a G protein and protein kinase A." American Journal of Physiology-Cell Physiology 266, no. 2 (1994): C536—C540. http://dx.doi.org/10.1152/ajpcell.1994.266.2.c536.

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The involvement of GTP-binding proteins (G proteins) in the regulation of the Cl- conductance in rat choroid plexus epithelial cells was investigated, using the whole cell patch-clamp technique. Intracellular application of a nonhydrolyzable GTP analogue, guanosine 5'-O-(3-thiotriphosphate) (GTP gamma S; 0.1-0.2 mM), evoked a transient increase in the Cl- conductance. The activated Cl- current exhibited inward rectification and was independent of time at hyperpolarizing or depolarizing voltage pulses. The effect of GTP gamma S was inhibited by a nonhydrolyzable GDP analogue, guanosine 5'-O-(2-

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Ikeda, Y., S. i. Kawahara, M. Taki, A. Kuno, T. Hasegawa, and K. Taira. "Synthesis of a novel histidine analogue and its efficient incorporation into a protein in vivo." Protein Engineering Design and Selection 16, no. 9 (2003): 699–706. http://dx.doi.org/10.1093/protein/gzg084.

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Soares, Luis R. B., Patricia L. Orr, Marvin R. Garovoy, and Gilles Benichou. "Differential Activation of T Cells by Natural Antigen Peptide Analogues: Influence on Autoimmune and Alloimmune In Vivo T Cell Responses." Journal of Immunology 160, no. 10 (1998): 4768–75. http://dx.doi.org/10.4049/jimmunol.160.10.4768.

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Abstract Recent studies using synthetic altered peptide ligands (Analogues) have led to the fine dissection of TCR-mediated T cell functions elicited by Ag recognition. Certain Analogues behave as full agonists of the antigenic peptide while others are partial agonists in that they only trigger selected T cell functions. Additionally, peptide Analogues can behave as antagonists by inhibiting functions of T cell clones when coincubated with the wild-type peptide. In fetal thymic organ cultures, synthetic altered peptide ligands can impact T cell repertoire selection. However, the influence of n

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Tornøe, Christian, Eva Johansson та Per-Olof Wahlund. "Divergent Protein Synthesis of Bowman–Birk Protease Inhibitors, their Hydrodynamic Behavior and Co-crystallization with α-Chymotrypsin". Synlett 28, № 15 (2017): 1901–6. http://dx.doi.org/10.1055/s-0036-1588840.

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A divergent protein synthesis strategy was executed to effectively synthesize Bowman–Birk protease inhibitor (BBI) analogues using native chemical ligation of peptide hydrazides. Grafting selected residues from a potent trypsin inhibitor, sunflower trypsin inhibitor-1, onto the α-chymotrypsin-binding loop of BBI, resulted in a fourfold improvement of α-chymotrypsin inhibition. The crystal structure of a synthetic BBI analogue co-crystallized with α-chymotrypsin confirmed the correct protein fold and showed a similar overall structure to unmodified BBI in complex with α-chymotrypsin. Dynamic li

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MITCHELL, John L. A., Carrie L. SIMKUS, Thynn K. THANE, et al. "Antizyme induction mediates feedback limitation of the incorporation of specific polyamine analogues in tissue culture." Biochemical Journal 384, no. 2 (2004): 271–79. http://dx.doi.org/10.1042/bj20040972.

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Spermidine, spermine and putrescine are essential for mammalian cell growth, and there has been a pervasive effort to synthesize analogues of these polyamines that will disrupt their function and serve as tools to inhibit cell proliferation. Recently, we demonstrated that a number of such polyamine analogues are also capable of inducing the regulatory protein AZ (antizyme). In the present study the incorporation of a few sample analogues [mimics of bis(ethyl)spermine] was shown to be significantly limited by a decrease in the Vmax for the polyamine transport system in response to analogue-indu

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Arueya, Gibson L., Bamidele S. Owosen, and Kazeem K. Olatoye. "Development of Texturized Vegetable Protein from Lima Bean (Phaseolus lunatus) and African Oil Bean Seed [Pentaclethrama crophylla (Benth)]: Optimization Approach." Acta Universitatis Cibiniensis. Series E: Food Technology 21, no. 1 (2017): 61–68. http://dx.doi.org/10.1515/aucft-2017-0007.

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AbstractAs part of measures to combat protein shortages in form of meat analogues, extrusion processing conditions for the development of Texturized Vegetable Protein (TVP) from under-utilized sources (Lima bean and African oil bean seed) are analysed. Optimum parameters for processing were established as being: barrel temperature (92.45°C), screw speed (101.48 rpm), feed moisture (59.63%) and African oil bean seed protein concentrates (AOBSPC) of 1%. Concentrations of essential amino-acids were also found to be significant (0.90-7.3%) with a near absence of anti-nutritional factors (0.0022–1.

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Bentin, Thomas, Ramin Hamzavi, Johan Salomonsson, Hervé Roy, Michael Ibba, and Peter E. Nielsen. "Photoreactive Bicyclic Amino Acids as Substrates for MutantEscherichia coliPhenylalanyl-tRNA Synthetases." Journal of Biological Chemistry 279, no. 19 (2004): 19839–45. http://dx.doi.org/10.1074/jbc.m401278200.

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Unnatural amino acids carrying reactive groups that can be selectively activated under non-invasive biologically benign conditions are of interest in protein engineering as biological tools for the analysis of protein-protein and protein-nucleic acids interactions. The double ring system phenylalanine analogues benzofuranylalanine and benzotriazolylalanine were synthesized, and their photolability was tested by UV irradiation at 254, 320, and 365 nm. Although both showed photo reactivity, benzofuranylalanine appeared as the most promising compound because this amino acid was activated by UVA (

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