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Articles de revues sur le sujet « Protein-Antibody recognition »

Auteur : Grafiati

Publié le 9 mars 2023

Mis à jour le 31 juillet 2025

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1

Ferrigno, Paul Ko. "Non-antibody protein-based biosensors." Essays in Biochemistry 60, no. 1 (2016): 19–25. http://dx.doi.org/10.1042/ebc20150003.

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Biosensors that depend on a physical or chemical measurement can be adversely affected by non-specific interactions. For example, a biosensor designed to measure specifically the levels of a rare analyte can give false positive results if there is even a small amount of interaction with a highly abundant but irrelevant molecule. To overcome this limitation, the biosensor community has frequently turned to antibody molecules as recognition elements because they are renowned for their exquisite specificity. Unfortunately antibodies can often fail when immobilised on inorganic surfaces, and alter

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Addis, Philip W., Catherine J. Hall, Shaun Bruton, et al. "Conformational Heterogeneity in Antibody-Protein Antigen Recognition." Journal of Biological Chemistry 289, no. 10 (2014): 7200–7210. http://dx.doi.org/10.1074/jbc.m113.492215.

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Pierce, Brian G., Zhen-Yong Keck, Patrick Lau, et al. "Global mapping of antibody recognition of the hepatitis C virus E2 glycoprotein: Implications for vaccine design." Proceedings of the National Academy of Sciences 113, no. 45 (2016): E6946—E6954. http://dx.doi.org/10.1073/pnas.1614942113.

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The E2 envelope glycoprotein is the primary target of human neutralizing antibody response against hepatitis C virus (HCV), and is thus a major focus of vaccine and immunotherapeutics efforts. There is emerging evidence that E2 is a highly complex, dynamic protein with residues across the protein that are modulating antibody recognition, local and global E2 stability, and viral escape. To comprehensively map these determinants, we performed global E2 alanine scanning with a panel of 16 human monoclonal antibodies (hmAbs), resulting in an unprecedented dataset of the effects of individual alani

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Huang, Jiachen, Darren Diaz, and Jarrod J. Mousa. "Antibody recognition of the Pneumovirus fusion protein trimer interface." PLOS Pathogens 16, no. 10 (2020): e1008942. http://dx.doi.org/10.1371/journal.ppat.1008942.

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Wang, Meryl, David Zhu, Jianwei Zhu, Ruth Nussinov, and Buyong Ma. "Local and global anatomy of antibody-protein antigen recognition." Journal of Molecular Recognition 31, no. 5 (2017): e2693. http://dx.doi.org/10.1002/jmr.2693.

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Kanaujia, G. V., S. Motzel, M. A. Garcia, P. Andersen, and M. L. Gennaro. "Recognition of ESAT-6 Sequences by Antibodies in Sera of Tuberculous Nonhuman Primates." Clinical Diagnostic Laboratory Immunology 11, no. 1 (2004): 222–26. http://dx.doi.org/10.1128/cdli.11.1.222-226.2004.

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ABSTRACT Previous work in our laboratory showed that the ESAT-6 protein of Mycobacterium tuberculosis and Mycobacterium bovis induces strong antibody responses in a large proportion (∼90%) of experimentally or naturally infected nonhuman primates. Here, the antibody response to ESAT-6 in tuberculous monkeys was characterized at the epitope level by measuring antibodies to overlapping, synthetic peptides spanning the ESAT-6 sequence. The antibody response against the COOH-terminal portion of the protein was the strongest in both experimentally and naturally infected animals. Moreover, these ant

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Margulies, David, and Andrew D. Hamilton. "Combinatorial protein recognition as an alternative approach to antibody-mimetics." Current Opinion in Chemical Biology 14, no. 6 (2010): 705–12. http://dx.doi.org/10.1016/j.cbpa.2010.07.017.

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Choi, Yujin, Hongwon Kim, So-Hyun Moon, Daehui Jeong, and Jong-Ho Kim. "Nanomaterial Artificial Antibodies for Effective Treatment of Breast Cancer." ECS Meeting Abstracts MA2025-01, no. 11 (2025): 963. https://doi.org/10.1149/ma2025-0111963mtgabs.

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Antibodies have been employed as recognition molecules that can bind to target molecules selectively for sensing and therapeutic applications. Antibody functions are unfortunately susceptible to changes in physical and chemical environments, and the processes required for antibody development and production are costly and time-consuming. Herein, antibody mimics emitting strong Raman scattering signals have been developed for therapy of breast cancer. To produce antibody mimics with strong affinity and high recognition selectivity to breast cancer, transition metal dichalcogenide nanosheets (TM

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Otlewski, J., and W. Apostoluk. "Structural and energetic aspects of protein-protein recognition." Acta Biochimica Polonica 44, no. 3 (1997): 367–87. http://dx.doi.org/10.18388/abp.1997_4392.

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Specific recognition between proteins plays a crucial role in a great number of vital processes. In this review different types of protein-protein complexes are analyzed on the basis of their three-dimensional structures which became available in recent years. The complexes which are analyzed include: those resulting from different types of recognition between proteinase and protein inhibitor (canonical inhibitors of serine proteinases, hirudin, inhibitors of cysteine proteinases, carboxypeptidase inhibitor), barnase-barstar, human growth hormone-receptor and antibody-antigen. It seems obvious

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Zhu, Jing, and Gang Sun. "Bio-functionalized nanofibrous membranes as a hybrid platform for selective antibody recognition and capturing." RSC Advances 5, no. 36 (2015): 28115–23. http://dx.doi.org/10.1039/c5ra01140j.

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Fuchs, Stephen M., Krzysztof Krajewski, Richard W. Baker, Victoria L. Miller, and Brian D. Strahl. "Influence of Combinatorial Histone Modifications on Antibody and Effector Protein Recognition." Current Biology 21, no. 1 (2011): 53–58. http://dx.doi.org/10.1016/j.cub.2010.11.058.

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Lak, Parnian, Spandana Makeneni, Robert J. Woods, and Todd L. Lowary. "Specificity of Furanoside-Protein Recognition through Antibody Engineering and Molecular Modeling." Chemistry - A European Journal 21, no. 3 (2014): 1138–48. http://dx.doi.org/10.1002/chem.201405259.

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Huang, Ao, Luning Zhang, Weiwei Li, Zeyu Ma, Shi Shuo, and Tianming Yao. "Controlled fluorescence quenching by antibody-conjugated graphene oxide to measure tau protein." Royal Society Open Science 5, no. 4 (2018): 171808. http://dx.doi.org/10.1098/rsos.171808.

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We report an ultrasensitive immunoassay for tau protein—a key marker of Alzheimer's disease. This sensing platform relies on graphene oxide (GO) surfaces conjugated with anti-human tau antibody to provide quantitative binding sites for the tau protein. The GO quenches standard fluorescein isothiocyanate labelled tau (tau-FITC) when tau protein and tau-FITC are both present and compete for the binding sites. This change in fluorescence signal can be used to quantitate tau protein. In contrast with traditional enzyme-linked immunosorbent assay (ELISA), our method does not require enzyme-linked s

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Wong, Hing C., Robert G. Newman, Warren D. Marcus, et al. "Novel antibody-like single-chain TCR antibody Fc fusion protein." Journal of Immunology 198, no. 1_Supplement (2017): 120.9. http://dx.doi.org/10.4049/jimmunol.198.supp.120.9.

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Abstract We have previously reported the construction of a fusion protein composed of a soluble single-chain T cell receptor genetically linked to the constant domain of the human IgG1 heavy chain (TCR-Ig). The antigen recognition portion of the protein binds to an unmutated peptide derived from human p53 (amino acids 264–272) presented in the context of HLA-A2.1, whereas the IgG1 Fc provides effector functions. The protein is capable of forming dimers, specifically staining tumor cells, and promoting target and effector cell conjugation. The protein also has potent antitumor effects against p

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Zhang, Zulei, Xingdi Zhang, Dechao Niu, Yongsheng Li, and Jianlin Shi. "Large-pore, silica particles with antibody-like, biorecognition sites for efficient protein separation." Journal of Materials Chemistry B 5, no. 22 (2017): 4214–20. http://dx.doi.org/10.1039/c7tb00886d.

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Pantophlet, Ralph, Meng Wang, Rowena O. Aguilar-Sino, and Dennis R. Burton. "The Human Immunodeficiency Virus Type 1 Envelope Spike of Primary Viruses Can Suppress Antibody Access to Variable Regions." Journal of Virology 83, no. 4 (2008): 1649–59. http://dx.doi.org/10.1128/jvi.02046-08.

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ABSTRACT The human immunodeficiency virus type 1 (HIV-1) envelope spike is a heavily glycosylated trimeric structure in which protein surfaces conserved between different HIV-1 isolates are particularly well hidden from antibody recognition. However, even variable regions on the spike tend to be less antigenic and immunogenic than one might have anticipated for external structures. Here we show that the envelope spike of primary viruses has an ability to restrict antibody recognition of variable regions. We show that access to an artificial epitope, introduced at multiple positions across the

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Easton, Donna M., Adam Smith, Sara Gomez Gallego, A. Ruth Foxwell, Allan W. Cripps, and Jennelle M. Kyd. "Characterization of a Novel Porin Protein from Moraxella catarrhalis and Identification of an Immunodominant Surface Loop." Journal of Bacteriology 187, no. 18 (2005): 6528–35. http://dx.doi.org/10.1128/jb.187.18.6528-6535.2005.

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ABSTRACT Moraxella catarrhalis is a gram-negative bacterium that is mainly responsible for respiratory tract infections. In this study we report a novel outer membrane protein (OMP), designated M35, with a molecular mass of 36.1 kDa. This protein was structurally homologous to classic gram-negative porins, such as OMP C from Escherichia coli and OMP K36 from Klebsiella pneumoniae, with a predicted structure of 8 surface loops and 16 antiparallel β-sheets. The DNA sequences of the genes from 18 diverse clinical isolates showed that the gene was highly conserved (99.6 to 100% of nucleotides), wi

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Liu, Hejun, Meng Yuan, Chang-Chun D. Lee, Nicholas C. Wu, and Ian A. Wilson. "Cross-neutralizing antibody recognition of sarbecoviruses." Journal of Immunology 206, no. 1_Supplement (2021): 114.02. http://dx.doi.org/10.4049/jimmunol.206.supp.114.02.

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Abstract The ongoing COVID-19 pandemic has been devasting to public health and global economy, with more than 82 million confirmed cases and 1.8 million deaths in 2020. Vaccines and antibody therapies are currently under emergency use authorization and provide hope to slow down and eventually stop the spread of SARS-CoV-2 infection. However, the continued identification of SARS-CoV-2 mutants as well as reciprocal transmission between human and farmed mink raise concerns on antigenic drift of SARS-CoV-2 and potential escape from current vaccines. Despite potent and versatile antibody responses

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Hui, G. S., S. P. Chang, H. Gibson, et al. "Influence of adjuvants on the antibody specificity to the Plasmodium falciparum major merozoite surface protein, gp195." Journal of Immunology 147, no. 11 (1991): 3935–41. http://dx.doi.org/10.4049/jimmunol.147.11.3935.

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Abstract The effect of adjuvants on the specificity of immune responses to the Plasmodium falciparum gp195 protein was investigated using adjuvant formulations based on synthetic muramyl dipeptide and monophosphoryl lipid A derivatives, in parallel with CFA and alum. Although these immunomodulators were as effective as CFA in inducing an antibody response to gp195, there were distinct differences in the recognition of B cell epitopes by these antibody populations. We have also demonstrated that MHC control of antibody specificity can be related to the adjuvant used for immunization. In general

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Branco, Ricardo J. F., Ana M. G. C. Dias, and Ana C. A. Roque. "Understanding the molecular recognition between antibody fragments and protein A biomimetic ligand." Journal of Chromatography A 1244 (June 2012): 106–15. http://dx.doi.org/10.1016/j.chroma.2012.04.071.

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Moraes, Adolfo H., Luca Simonelli, Mattia Pedotti, Fabio C. L. Almeida, Luca Varani, and Ana P. Valente. "Antibody Binding Modulates Conformational Exchange in Domain III of Dengue Virus E Protein." Journal of Virology 90, no. 4 (2015): 1802–11. http://dx.doi.org/10.1128/jvi.02314-15.

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ABSTRACTDomain III of dengue virus E protein (DIII) participates in the recognition of cell receptors and in structural rearrangements required for membrane fusion and ultimately viral infection; furthermore, it contains epitopes for neutralizing antibodies and has been considered a potential vaccination agent. In this work, we addressed various structural aspects of DIII and their relevance for both the dengue virus infection mechanism and antibody recognition. We provided a dynamic description of DIII at physiological and endosomal pHs and in complex with the neutralizing human antibody DV32

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Lyashchenko, Konstantin, Roberto Colangeli, Michel Houde, Hamdan Al Jahdali, Dick Menzies, and Maria Laura Gennaro. "Heterogeneous Antibody Responses in Tuberculosis." Infection and Immunity 66, no. 8 (1998): 3936–40. http://dx.doi.org/10.1128/iai.66.8.3936-3940.1998.

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ABSTRACT Antibody responses during tuberculosis were analyzed by an enzyme-linked immunosorbent assay with a panel of 10 protein antigens of Mycobacterium tuberculosis. It was shown that serum immunoglobulin G antibodies were produced against a variety of M. tuberculosis antigens and that the vast majority of sera from tuberculosis patients contained antibodies against one or more M. tuberculosis antigens. The number and the species of serologically reactive antigens varied greatly from individual to individual. In a given serum, the level of specific antibodies also varied with the antigen ir

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Tokatlian, Talar, Benjamin J. Read, Christopher A. Jones, et al. "Innate immune recognition of glycans targets HIV nanoparticle immunogens to germinal centers." Science 363, no. 6427 (2018): 649–54. http://dx.doi.org/10.1126/science.aat9120.

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In vaccine design, antigens are often arrayed in a multivalent nanoparticle form, but in vivo mechanisms underlying the enhanced immunity elicited by such vaccines remain poorly understood. We compared the fates of two different heavily glycosylated HIV antigens, a gp120-derived mini-protein and a large, stabilized envelope trimer, in protein nanoparticle or “free” forms after primary immunization. Unlike monomeric antigens, nanoparticles were rapidly shuttled to the follicular dendritic cell (FDC) network and then concentrated in germinal centers in a complement-, mannose-binding lectin (MBL)

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Rauch, J., and AS Janoff. "Antibodies against Phospholipids other than Cardiolipin: Potential Roles for Both Phospholipid and Protein." Lupus 5, no. 5 (1996): 498–502. http://dx.doi.org/10.1177/096120339600500534.

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Autoantibodies to phospholipids other than cardiolipin have received less attention, to date, than anti-cardiolipin antibodies. This review focuses on these antibodies and potential roles for both phospholipid and protein in their reactivity. We review data in the literature indicating that antibodies to phosphatidylethanolamine and some lupus anticoagulant antibodies recognize phospholipid-binding proteins in association with phospholipid. Kininogens appear to be involved in the binding of antibodies to phosphatidylethanolamine, while phosphatidylserine-binding proteins, such as prothrombin a

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Kumar, Vasanthi U., S. Shishupala, H. S. Shetty, and S. Umesh-Kumar. "Serological evidence for the occurrence of races in Sclerospora graminicola and identification of a race-specific surface protein involved in host recognition." Canadian Journal of Botany 71, no. 11 (1993): 1467–71. http://dx.doi.org/10.1139/b93-177.

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A protein specific to a virulent pathotype of the downy mildew pathogen Sclerospora graminicola was identified on the Western transfers using the cross-adsorbed antibody. This protein was approximately 90 kDa and without subunits. Serological agglutination studies localized the protein on the cell wall surfaces. Enzyme linked immunosorbent assay and dot blotting using the specific antibodies identified distinct races of the pathogen. Immunofluorescence studies and enzyme linked immunosorbent assay using suspension cultures of host cells showed that the protein bound to the cells of susceptible

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Jian, Jhih-Wei, Hong-Sen Chen, Yi-Kai Chiu, et al. "Effective binding to protein antigens by antibodies from antibody libraries designed with enhanced protein recognition propensities." mAbs 11, no. 2 (2019): 373–87. http://dx.doi.org/10.1080/19420862.2018.1550320.

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Shankarappa, Basavaraju, and Sukanta K. Dutta. "Production and characterization of monoclonal antibodies to Ehrlichia risticii." American Journal of Veterinary Research 50, no. 7 (1989): 1145–49. https://doi.org/10.2460/ajvr.1989.50.07.1145.

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SUMMARY Hybridomas producing monoclonal antibodies to Ehrlichia risticii were developed to provide a means of molecular investigation of the biochemical and immunopathologic characteristics of the organism. All of 6 stable monoclonal antibodies obtained were IgG isotypes. The ascitic fluid titers induced by the hybridomas ranged from 102 to 107. Competitive binding experiments conducted by elisa and binding of labeled protein A to antigen-antibody complexes indicated competition among monoclonal antibodies. Two monoclonal antibodies (HybI and 14D4) were reactive in an indirect fluorescent anti

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Kamata, Teddy, Mohan Natesan, Kelly Warfield, M. Javad Aman, and Robert G. Ulrich. "Determination of Specific Antibody Responses to the Six Species of Ebola and Marburg Viruses by Multiplexed Protein Microarrays." Clinical and Vaccine Immunology 21, no. 12 (2014): 1605–12. http://dx.doi.org/10.1128/cvi.00484-14.

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ABSTRACTInfectious hemorrhagic fevers caused by the Marburg and Ebola filoviruses result in human mortality rates of up to 90%, and there are no effective vaccines or therapeutics available for clinical use. The highly infectious and lethal nature of these viruses highlights the need for reliable and sensitive diagnostic methods. We assembled a protein microarray displaying nucleoprotein (NP), virion protein 40 (VP40), and glycoprotein (GP) antigens from isolates representing the six species of filoviruses for use as a surveillance and diagnostic platform. Using the microarrays, we examined se

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Nicholls, Ian A., and Jesper G. Wiklander. "Towards Peptide and Protein Recognition by Antibody Mimicking Synthetic Polymers – Background, State of the Art, and Future Outlook." Australian Journal of Chemistry 73, no. 4 (2020): 300. http://dx.doi.org/10.1071/ch20020.

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Antibody–peptide/protein interactions are instrumental for many processes in the pharmaceutical and biotechnology industries and as tools for biomedical and biochemical research. The recent development of molecularly imprinted polymer nanoparticles displaying antibody-like recognition of peptides and proteins offers the possibility for substituting antibodies with these robust materials for applications where the structural integrity and function of antibodies is compromised by temperature, pH, solvent, etc. The background to the development of this class of antibody-mimicking material and the

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Milich, D. R., A. McLachlan, F. V. Chisari, and G. B. Thornton. "Nonoverlapping T and B cell determinants on an hepatitis B surface antigen pre-S(2) region synthetic peptide." Journal of Experimental Medicine 164, no. 2 (1986): 532–47. http://dx.doi.org/10.1084/jem.164.2.532.

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We have examined T cell recognition of a hepatitis B surface antigen (HBsAg), pre-S(2)-region synthetic peptide, p120-145, in terms of fine specificity, H-2-linked genetic influences, comparison to antibody binding, and relevance to T cell recognition of the native protein. We showed that the immune response to the synthetic peptide is regulated by H-2-linked genes, but that the pattern of H-2 restriction differed from that observed for the native anti-pre-S(2) response. Dominant and nonoverlapping T cell and B cell recognition sites were identified on the synthetic peptide p120-145. T cell re

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Crispin, Max, Andrew B. Ward, and Ian A. Wilson. "Structure and Immune Recognition of the HIV Glycan Shield." Annual Review of Biophysics 47, no. 1 (2018): 499–523. http://dx.doi.org/10.1146/annurev-biophys-060414-034156.

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Vaccine design efforts against the human immunodeficiency virus (HIV) have been greatly stimulated by the observation that many infected patients eventually develop highly potent broadly neutralizing antibodies (bnAbs). Importantly, these bnAbs have evolved to recognize not only the two protein components of the viral envelope protein (Env) but also the numerous glycans that form a protective barrier on the Env protein. Because Env is heavily glycosylated compared to host glycoproteins, the glycans have become targets for the antibody response. Therefore, considerable efforts have been made in

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Ramsden, J. J., and P. Schneider. "Membrane insertion and antibody recognition of a glycosylphosphatidylinositol-anchored protein: an optical study." Biochemistry 32, no. 2 (1993): 523–29. http://dx.doi.org/10.1021/bi00053a017.

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Kang, Hyo Jin, Young Ji Kang, Young-Mi Lee, Hyun-Hee Shin, Sang J. Chung, and Sebyung Kang. "Developing an antibody-binding protein cage as a molecular recognition drug modular nanoplatform." Biomaterials 33, no. 21 (2012): 5423–30. http://dx.doi.org/10.1016/j.biomaterials.2012.03.055.

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Lyashchenko, Konstantin P., John M. Pollock, Roberto Colangeli, and Maria Laura Gennaro. "Diversity of Antigen Recognition by Serum Antibodies in Experimental Bovine Tuberculosis." Infection and Immunity 66, no. 11 (1998): 5344–49. http://dx.doi.org/10.1128/iai.66.11.5344-5349.1998.

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ABSTRACT Tuberculosis in cattle remains a major zoonotic and economic problem in many countries. The standard diagnostic assay for bovine tuberculosis, the intradermal tuberculin test, has low accuracy. Therefore, alternative immunodiagnostic methods, such as serological assays, are needed for detection of infected animals. Development of an accurate serodiagnostic test requires a detailed understanding of the humoral immune responses during bovine tuberculosis and, in particular, identification of the key antigens of Mycobacterium bovisinvolved in antibody production. In this study, we charac

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Schwarze, Mandy, Daniela Volke, Juan Camilo Rojas Echeverri, et al. "Influence of Mutations and N-Glycosylation Sites in the Receptor-Binding Domain (RBD) and the Membrane Protein of SARS-CoV-2 Variants of Concern on Antibody Binding in ELISA." Biology 13, no. 4 (2024): 207. http://dx.doi.org/10.3390/biology13040207.

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Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) can infect human cells by first attaching to the ACE-2 receptor via its receptor-binding domain (RBD) in the spike protein. Here, we report the influence of N-glycosylation sites of the RBD and the membrane (M) protein on IgG antibody binding in serum samples from patients infected with the original SARS-CoV-2 strain in Germany. The RBDs of the wildtype, alpha, beta, gamma, and kappa variants expressed in HEK293S GnTI− cells were all N-glycosylated at Asn331, Asn334, Asn343, and Asn360 or Asn370, whereas the M-protein was glycosylate

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Ntchobo, Hyacinthe, Holly Rothermel, Wambui Chege, Allen C. Steere, and Jenifer Coburn. "Recognition of Multiple Antibody Epitopes throughout Borrelia burgdorferi p66, a Candidate Adhesin, in Patients with Early or Late Manifestations of Lyme Disease." Infection and Immunity 69, no. 3 (2001): 1953–56. http://dx.doi.org/10.1128/iai.69.3.1953-1956.2001.

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ABSTRACT Antibody responses to p66, a candidate integrin ligand ofBorrelia burgdorferi, were studied in 79 patients with early or late manifestations of Lyme disease. The central portion of p66 was previously shown to contain all of the information required for specific recognition of β3-chain integrins, but work by others had suggested that the C-terminal portion of the protein contains a single surface-exposed, immunodominant loop. In examining antibody responses to full-length p66 and to three overlapping fragments of the protein, we found that the majority of Lyme disease patients had immu

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Lee, Bok-Soo, Michelle Connole, Zuoquin Tang, Nancy L. Harris, and Jae U. Jung. "Structural Analysis of the Kaposi's Sarcoma-Associated Herpesvirus K1 Protein." Journal of Virology 77, no. 14 (2003): 8072–86. http://dx.doi.org/10.1128/jvi.77.14.8072-8086.2003.

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ABSTRACT The K1 protein of Kaposi's sarcoma-associated herpesvirus (KSHV) efficiently transduces extracellular signals to elicit cellular activation events through its cytoplasmic immunoreceptor tyrosine-based activation motif (ITAM). In addition, the extracellular domain of K1 demonstrates regional homology with the immunoglobulin (Ig) family and contains conserved regions (C1 and C2) and variable regions (V1 and V2). To generate mouse monoclonal antibodies directed against the KSHV K1 protein, BALB/c mice were primed and given boosters with K1 protein purified from mammalian cells. Twenty-ei

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Genova-Kalou, Petia, Georgi Dyankov, Hristo Kisov, et al. "EVALUATION OF INTERACTIONS OF SARS-COV-2 STRUCTURAL PROTEINS WITH SPECIFIC ANTIBODIES BY SPR ASSAY." PROBLEMS of Infectious and Parasitic Diseases 50, no. 3 (2023): 29–35. http://dx.doi.org/10.58395/peg69k16.

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Background: The World Health Organization admitted that the vaccination against Covid 19 limited the deaths, but not the spread of the disease. This requires a method allowing a specific, rapid and accurate diagnosis of the disease. We report a SPR assay that meets the requirements and can be applied no only for SARS Cov-2 diagnosis but as a tool for early diagnosis of otherinfections. Methods: Surface plasmon resonance (SPR) method was used to identify the binding of S/N protein to monoclonal antibodies. N-protein monoclonal antibody (NP mAb), S-protein monoclonal antibody (SP mAb), and recep

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Lee, Sin, Yujin Choi, and Jong-Ho Kim. "(Invited) Nanosheet Antibody Mimics for Diagnosis and Therapy of Bacterial Infections." ECS Meeting Abstracts MA2024-02, no. 11 (2024): 1535. https://doi.org/10.1149/ma2024-02111535mtgabs.

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Antibodies are widely employed as recognition elements in diagnosis and therapy of various diseases [1], however, they have some limitations such as poor stability, long discovery time, and high cost. Herein, we present a facile approach to create nanosheet antibody mimics with multivalent peptide recognition phases and luminescent rigid transition metal dichalcogenide (TMD) nanosheets for the selective recognition, detection, and eradication of pathogenic bacteria. Tripeptides with a nitriloacetate-Cu group were spontaneously assembled on TMD nanosheets via coordinate bonding, providing a var

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Guo, Xin-Yu, Xiao-Dong Gao, and Morihisa Fujita. "Sulfation of a FLAG tag mediated by SLC35B2 and TPST2 affects antibody recognition." PLOS ONE 16, no. 5 (2021): e0250805. http://dx.doi.org/10.1371/journal.pone.0250805.

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A FLAG tag consisting of DYKDDDDK is an epitope tag that is frequently and widely used to detect recombinant proteins of interest. In this study, we performed a CRISPR-based genetic screening to identify factors involved in the detection of a FLAG-tagged misfolded model protein at the cell surface. In the screening, SLC35B2, which encodes 3’-phosphoadenosine-5’-phosphosulfate transporter 1, was identified as the candidate gene. The detection of FLAG-tagged misfolded proteins at the cell surface was significantly increased in SLC35B2-knockout cells. Furthermore, protein tyrosine sulfation media

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Giannoccaro, Maria Pia, Sarah J. Crisp, and Angela Vincent. "Antibody-mediated central nervous system diseases." Brain and Neuroscience Advances 2 (January 2018): 239821281881749. http://dx.doi.org/10.1177/2398212818817497.

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Antibody-mediated central nervous system diseases are a relatively new area of clinical neuroscience with growing impact. Their recognition has challenged the dogma of the blood–brain barrier preventing antibody access into the central nervous system. The antibodies discovered so far are mainly against neurotransmitter receptors (e.g. N-methyl-d-aspartate and glycine receptors) and ion channel–associated proteins (leucine-rich glioma inactivated protein 1 and contactin-associated protein 2) and are expressed on the surface of neuronal synapses and elsewhere. The disorders are reversible with i

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Xainli, Jia, Jennifer L. Cole-Tobian, Moses Baisor, et al. "Epitope-Specific Humoral Immunity to Plasmodium vivax Duffy Binding Protein." Infection and Immunity 71, no. 5 (2003): 2508–15. http://dx.doi.org/10.1128/iai.71.5.2508-2515.2003.

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ABSTRACT Erythrocyte invasion by Plasmodium vivax is completely dependent on binding to the Duffy blood group antigen by the parasite Duffy binding protein (DBP). The receptor-binding domain of this protein lies within a cysteine-rich region referred to as region II (DBPII). To examine whether antibody responses to DBP correlate with age-acquired immunity to P. vivax, antibodies to recombinant DBP (rDBP) were measured in 551 individuals residing in a village endemic for P. vivax in Papua New Guinea, and linear epitopes mapped in the critical binding region of DBPII. Antibody levels to rDBPII i

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Fassler, Michael, Sagi Tshori, Yaron Barac, Dawn E. Bowles, Clara Benaim, and Jacob George. "Dual Targeting of Soluble Oligomeric and Aggregated Transthyretin with a Monoclonal Antibody Ameliorates Experimental Neuropathy." Biology 11, no. 10 (2022): 1509. http://dx.doi.org/10.3390/biology11101509.

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ATTR amyloidosis comprises a spectrum of multiple clinical presentations, including, predominantly, neuropathy and cardiomyopathy. The common triggering pathogenic protein is misfolded transthyretin, a carrier protein that destabilizes misfolds and assembles into mature amyloid fibrils. The current management of ATTR amyloidosis includes the use of agents that stabilize TTR or attenuate its liver inducible production. Herein, we tested the hypothesis that a monoclonal antibody targeting the soluble oligomeric as well as the aggregated TTR would influence experimental neuropathy. We have shown

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Calle, J. M., E. H. Nardin, P. Clavijo, et al. "Recognition of different domains of the Plasmodium falciparum CS protein by the sera of naturally infected individuals compared with those of sporozoite-immunized volunteers." Journal of Immunology 149, no. 8 (1992): 2695–701. http://dx.doi.org/10.4049/jimmunol.149.8.2695.

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Abstract The fine specificities of antibodies to the circumsporozoite (CS) protein of Plasmodium falciparum, present in the sera of volunteers immunized with irradiated P. falciparum sporozoites, were defined and compared to those of sera from persons living in a malaria-endemic area in West Africa. The specificity of these anti-CS antibodies was determined by ELISA, using recombinant proteins and synthetic peptides containing repeat and nonrepeat sequences of this CS protein. All 10 serum samples of the five sporozoite-immunized volunteers displayed very high antibody titers to the immunodomi

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Hobson-Peters, Jody, Philip Toye, Melissa D. Sánchez, et al. "A glycosylated peptide in the West Nile virus envelope protein is immunogenic during equine infection." Journal of General Virology 89, no. 12 (2008): 3063–72. http://dx.doi.org/10.1099/vir.0.2008/003731-0.

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Using a monoclonal antibody directed to domain I of the West Nile virus (WNV) envelope (E) protein, we identified a continuous (linear) epitope that was immunogenic during WNV infection of horses. Using synthetic peptides, this epitope was mapped to a 19 aa sequence (WN19: E147–165) encompassing the WNV NY99 E protein glycosylation site at position 154. The inability of WNV-positive horse and mouse sera to bind the synthetic peptides indicated that glycosylation was required for recognition of peptide WN19 by WNV-specific antibodies in sera. N-linked glycosylation of WN19 was achieved through

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Smith, Kelly, Americo Lopez-Yglesias, Chunchi Lu, Xiaodan Zhao, and Roland Strong. "Flagellin’s hypervariable D2/D3 domain is required for robust anti-flagellin primary antibody responses (VAC11P.1065)." Journal of Immunology 194, no. 1_Supplement (2015): 212.5. http://dx.doi.org/10.4049/jimmunol.194.supp.212.5.

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Abstract Bacterial flagellin is a well-known agonist of the innate immune system. Several clinical trials investigating flagellin fusion proteins have demonstrated promising results for inducing protective immunity towards influenza virus, which has been largely attributed to flagellin activation of TLR5. Our lab recently demonstrated that the Salmonella enterica serovar Typhimurium flagellin protein, FliC, induces antibody responses in mice through a third pathway that is independent of TLR5, Casp1, and MyD88. Herein, we define the structural features of FliC that contribute to this third pat

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Lipp, J., and B. Dobberstein. "Signal recognition particle-dependent membrane insertion of mouse invariant chain: a membrane-spanning protein with a cytoplasmically exposed amino terminus." Journal of Cell Biology 102, no. 6 (1986): 2169–75. http://dx.doi.org/10.1083/jcb.102.6.2169.

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Invariant (Ii) chain is a membrane-spanning protein that is found associated intracellularly with class II histocompatibility antigens. In the endoplasmic reticulum Ii chain spans the membrane and exposes the NH2 terminus on the cytoplasmic and the COOH terminus on the lumenal side. This orientation across the membrane is demonstrated directly with the monoclonal antibody In-1, which exclusively recognizes the NH2 terminal cytoplasmically exposed part of Ii chain. Membrane insertion of Ii chain requires signal recognition particle and docking protein. When tested in a wheat germ cell free syst

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Kobayashi, Tamaki, Kazuki Yamazaki, Junki Shinada, Masataka Mizunuma, Kazuhiro Furukawa, and Yoshiro Chuman. "Identification of Inhibitors of the Disease-Associated Protein Phosphatase Scp1 Using Antibody Mimetic Molecules." International Journal of Molecular Sciences 25, no. 7 (2024): 3737. http://dx.doi.org/10.3390/ijms25073737.

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Protein phosphorylation is a prevalent translational modification, and its dysregulation has been implicated in various diseases, including cancer. Despite its significance, there is a lack of specific inhibitors of the FCP/SCP-type Ser/Thr protein phosphatase Scp1, characterized by high specificity and affinity. In this study, we focused on adnectin, an antibody-mimetic protein, aiming to identify Scp1-specific binding molecules with a broad binding surface that target the substrate-recognition site of Scp1. Biopanning of Scp1 was performed using an adnectin-presenting phage library with a ra

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Martin, D. R., N. Ruijne, L. McCallum, J. O'Hallahan, and P. Oster. "The VR2 Epitope on the PorA P1.7-2,4 Protein Is the Major Target for the Immune Response Elicited by the Strain-Specific Group B Meningococcal Vaccine MeNZB." Clinical and Vaccine Immunology 13, no. 4 (2006): 486–91. http://dx.doi.org/10.1128/cvi.13.4.486-491.2006.

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ABSTRACT A protracted epidemic of group B meningococcal disease in New Zealand led to the testing of a strain-specific tailor-made vaccine, MeNZB. Immunogenicity levels achieved during age group trials enabled New Zealand's regulatory authority to grant licensure to deliver MeNZB to all individuals under age 20. During the trials target strains for serum bactericidal antibody measurements included the vaccine target strain NZ98/254 and two comparator epidemic-type strains (NZ94/167 and NZ02/09). In this study, 12 other strains differing variously from the vaccine strain by their capsular group

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Tomasini, BR, and DF Mosher. "Conformational states of vitronectin: preferential expression of an antigenic epitope when vitronectin is covalently and noncovalently complexed with thrombin-antithrombin III or treated with urea." Blood 72, no. 3 (1988): 903–12. http://dx.doi.org/10.1182/blood.v72.3.903.903.

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Abstract A difference in recognition of the adhesive glycoprotein vitronectin (also called S-protein, serum spreading factor, and epibolin) by monoclonal antibody 8E6 (Hayman EG, et al, Proc Natl Acad Sci USA 80:4003, 1983) was investigated using a competitive enzyme- immunosorbent assay and immunoaffinity chromatography. Recognition of vitronectin in serum was approximately 50-fold greater than recognition of vitronectin in plasma. Recognition of vitronectin incubated with heparin, thrombin-antithrombin III complex, or heparin and thrombin- antithrombin III complex together was 2.5-, 7-, or 3

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