Littérature scientifique sur le sujet « Radish ( R. sativus parvus) »

Small radish and radish are economically important root crops that represent an integral part of a healthy human diet. The world collection of Raphanus L. root crops, maintained in the VIR genebank, includes 2810 accessions from 75 countries around the world, of which 2800 (1600 small radish, 1200 radish) belong to R. sativus species, three to R. raphanistrum, three to R. landra, and four to R. caudatus. It is necessary to systematically investigate the historical and modern gene pool of root-bearing plants of R. sativus and provide new material for breeding. The material for our research was a set of small radish and radish accessions of various ecological groups and different geographical origin, fully covering the diversity of the species. The small radish subset included 149 accessions from 37 countries, belonging to 13 types of seven varieties of European and Chinese subspecies. The radish subset included 129 accessions from 21 countries, belonging to 18 types of 11 varieties of European, Chinese, and Japanese subspecies. As a result of the evaluation of R. sativus accessions according to phenological, morphological, and biochemical analyses, a wide variation of these characteristics was revealed, which is due to the large genetic diversity of small radish and radish of various ecological and geographical origins. The investigation of the degree of variation regarding phenotypic and biochemical traits revealed adaptive stable and highly variable characteristics of R. sativus accessions. Such insights are crucial for the establishment and further use of trait collections. Trait collections facilitate germplasm use and contribute significantly to the preservation of genetic diversity of the gene pool.

Twelve amplified fragment length polymorphism (AFLP) primer combinations and 10 inter-simple sequence repeat (ISSR) primers were applied to estimate genetic diversity among 68 varieties of cultivated radish (Raphanus sativus L.). The material consisted of open-pollinated varieties, inbred lines, diploid and a few tetraploid hybrid varieties of garden radish [R. sativus var. sativus DC. convar. radicula (DC.) Alef.] and black radish [R. sativus var. niger (Mill.) Pers.]. Two accessions of uncultivated relatives of radish that as weeds cause serious contamination during the process of hybrid radish production were added to the analyses. Polymorphic fragments were scored for calculation of Jaccard's coefficient of genetic similarity (GS). Substantial level of genetic variability (average AFLP-based GS = 0.70; average ISSR-based GS = 0.61) was detected in the available germplasm of cultivated radish. Cluster analyses separated two weedy species from the cultivated germplasm. Within cultivated material, black radish and french breakfast radish types formed separate clusters. Based on AFLP data, a principal coordinate analysis (PCoA) and model-based approach revealed the genetic structure within cultivated radish germplasm and indicated the existence of divergent pools. Although the model-based approach did not separate black radish from french breakfast radish varieties, it offered a clear sub-division within garden radish germplasm. The results of this study may be relevant for hybrid radish breeding.

Radish and small radish (Raphanus sativus L.) are popular and widely cultivated root vegetables in the world, which occupy an important place in human nutrition. Edaphic stressors have a significant impact on their productivity and quality. The main factor determining the phytotoxicity of acidic soils is the increased concentration of mobile aluminum ions in the soil solution. The accumulation of aluminum in root tissues disrupts the processes of cell division, initiation and growth of the lateral roots, the supply of plants with minerals and water. The study of intraspecific variation in aluminum resistance of R. sativus is an important stage for the breeding of these crops. The purpose of this work was to study the genetic diversity of R. sativus crops including 109 accessions of small radish and radish of various ecological and geographical origin, belonging to 23 types, 14 varieties of European, Chinese and Japanese subspecies on aluminum tolerance. In the absence of a rapid assessment methodology specialized for the species studied, a method is used to assess the aluminum resistance of cereals using an eriochrome cyanine R dye, which is based on the recovery or absence of restoration of mitotic activity of the seedlings roots subjected to shock exposure to aluminum. The effect of various concentrations on the vital activity of plants was revealed: a 66-mM concentration of AlCl3 · 6Н2О had a weak toxic effect on R. sativus accessions slowing down root growth; 83 mM contributed to a large differentiation of the small radish accessions and to a lesser extent for radish; 99 mM inhibited further root growth in 13.0 % of small radish accessions and in 7.3 % of radish and had a highly damaging effect. AlCl3 · 6Н2О at a concentration of 99 mM allowed us to identify the most tolerant small radish and radish accessions that originate from countries with a wide distribution of acidic soils. In a result, it was possible to determine the intraspecific variability of small radish and radish plants in the early stages of vegetation and to identify genotypes that are contrasting in their resistance to aluminum. We recommend the AlCl3 · 6Н2О concentration of 83 mM for screening the aluminum resistance of small radish and 99 mM for radish. The modified method that we developed is proposed as a rapid diagnosis of aluminum tolerance for the screening of a wide range of R. sativus genotypes and a subsequent study of contrasting forms during a longer cultivation of plants in hydroponic culture (including elemental analysis of roots and shoots, contrasting in resistance of accessions) as well as reactions of plants in soil conditions.

Shoots of field-grown plants of European radish (Raphanus sativus L. `Comet'), Japanese radish (daikon) (R. sativus L. `Mino-wase'), and Asian turnip (Brassica rapa L. `Hinona') were brushed twice daily, 40 cycles (back-and-forth strokes equal one cycle), for 8 (short-term) or 13, 21, or 14 days (long-term), respectively. European turnip (B. rapa L. `Hikari-kabu') plants were grown in a raised bed in an uncovered plastic house and were brushed for 9 or 21 days. Short-term brushing reduced root dry weight and the root: shoot dry weight ratio (R: S) of European radish, whereas the treatment increased these measurements for Asian turnip and European turnip. Short-term brushing had no effect on growth of Japanese radish plants, but increased root dry weight gain by both turnip cultivars, thereby increasing their R: S ratios. Lung-term brushing reduced root dry weight gain and the R: S ratio of European radish and root dry weight of Asian turnip but had no effect on these measurements for the other crops. Long-term brushing reduced cracking of European radish roots, thereby resulting in a higher proportion of oblong (medium quality) roots. Consequently, the percentage of marketable-quality roots of European radish was increased by brushing. Lung-term brushing increased the percentage of medium-sized roots of Asian turnip at the expense of large-sized roots. Brushing had no effect on root quality of European turnip.

Vegetables have great significance and importance as they are not only useful as food but on the other hand, vegetables are a vital source in the treatment and prevention of various ailments, a powerful source of minerals, vitamins, antioxidants, fibers, and amino acids, therefore, help to buildup and heal the body. Mainly concerning cruciferous vegetables, have been famous for marked effects of cardio-protective, antimicrobial, and anti-carcinogenic. The environments of Pakistan are amicable for vegetable production. Raphanus sativus generally named radish is a widespread plant all over the world belongs to the Brassicaceae family. Edible R sativus L. Var. caudatus are green color seed pods, utilized for properties attributed to Raphanus. Different chemical constituents are identified from radish. Various researches show that the whole radish plant has significant pharmacological activity. The current review expands ontypes, phytochemical components, and pharmacological potential and toxic outcomes of the radish plant Keywords: Radish, varieties, constituents, pharmacology, uses, toxicity

Hosh, M., S. Tabbache, D. Haddad and H. Habak. 2022. The Effect of Dried Powdered Leaves of Radish (Rhaphanus sativus L.) in Decreasing the Parasitism of Orobanche ramosa L. on Tomato (Solanum lycopersicum L.) Grown in Greenhouses. Arab Journal of Plant Protection, 40(3): 280-285. https://doi.org/10.22268/AJPP-40.3.280285 The allelopathic effect of dried leaves powder mixed with soil at concentrations of 1, 2, 4% of Raphanus sativus on both parasitic weed Orobanche ramosa L. germination and growth of tomato Solanum lycopersicum L. plants was studied. The results obtained showed that the R. sativus powder at 2 and 4% concentrations, was effective in decreasing the average number of O. ramosa tubers which reached 4.20 and 2.00 tubers/plant, respectively, compared to the control which was 48.80 tubers/plant, and decreased the average length of Orobanche branches to 2.67 and 0.53 cm, respectively, compared to 10.33 cm for the control. Furthermore, Orobanche dry weight reached 0.80 and 0.02 g, respectively, in response to the two concentrations of the radish powder, compared to 4.24 g for the control. There was no negative effect on the growth and weight of tomato plants when treated with radish powder, suggesting the effectiveness of radish leaves powder added to the soil as an effective biological material in reducing the incidence of branched broomrape Orobanche ramose L., and protecting tomato plant Solanum lycopersicum L. from this parasitic weed. Keywords: Solanum lycopersicum, Orobanche ramose, Allelopathic effect, Raphanus sativus, radish

Objective: Cruciferous herb plant Weixian Radish (Raphanus Sativus L.), also known as Lai Fu, is a kind of popularly eatable fruit and vegetable. The present paper describes the isolation and characterization of two compounds isolated from Weixian Radish. Methods: The methanol extraction was separated by column chromatography. Obtained compounds were identified with physical and chemical properties and NMR. Results: Two compounds were isolated from the extract of Raphanus Sativus L. for the first time and their structures were identified as β-sitosterol and 1-O-(β-D-glucopyranosyl)-(2S, 3S, 4R, 8E)-2-[(2'R)-2'-hydroxyltetracos-15'-enoylamino]-8-octa-decene-1, 3, 4-triol, which have many biologically active effects.

The hypothesis that host plants exert selection pressure on Heterodera schachtii populations was tested. Host selection of genotypes from three genetically distinct isolates of H. schachtii was assessed using cabbage, sugar beet, oilseed radish (Raphanus sativus), and white mustard (Sinapis alba). The plants represent a range of susceptibility to H. schachtii and included R. sativus and S. alba, because cultivars of those species have been used as trap crops for H. schachtii in Europe. Genotypic differences in amplified fragment length polymorphism (AFLP) and random amplified polymorphic DNA (RAPD) markers were detected among the isolates after they reproduced on the different hosts. The poorest host plant, R. sativus, resulted in the greatest number of changes in both AFLP and RAPD markers. Oilseed radish selected nematode genotypes in less than four nematode generations. The nematode population genotypes detected by RAPD analyses after selection on oilseed radish were observed even after nematode populations were transferred back to the other three hosts. The genetic markers that were detected after selection were influenced by the genotypes of the original nematode isolates. The results indicate the utility of RAPDs and AFLPs for identifying and monitoring intraspecific genetic variability in nematodes and for understanding nematode population responses to host plants. Nematode management practices such as using resistant cultivars may alter gene frequencies, thereby reducing the efficacy of the tactic and exacerbating the nematode's potential to damage subsequent crops.

Vegetable grafting has primarily been used in the commercial production of high-value crops in the Solanaceae and Cucurbitaceae families. In this study, we explored the feasibility of making a novel graft between pac choi (Brassica rapa L. var. chinensis) and daikon radish (Raphanus sativus L. var. longipinnatus) to create a plant with harvestable pac choi leafy vegetable above-ground, and a daikon radish taproot below-ground. ‘Mei Qing Choi’ pac choi (scion) was grafted onto ‘Bora King’ daikon radish (rootstock). Grafted pac choi–daikon radish plants did not show a decrease in SPAD value, canopy size, leaf number, leaf area, or above-ground weight compared with self-grafted pac choi plants. However, taproot formation was reduced in grafted pac choi–daikon radish plants, as shown by decreased taproot length, diameter, fresh weight, and dry weight compared with non- and self-grafted daikon radish plants. Surprisingly, grafting with radish increased the photosynthetic rate of the pac choi. This pilot study demonstrated the potential of creating a new pac choi–daikon radish vegetable product to help save growing space and minimize waste at consumption, as pac choi roots are not eaten and radish leaves are usually discarded. The inter-generic grafting between B. rapa var. chinensis and R. sativus var. longipinnatus could also provide a unique model system to help elucidate scion-rootstock synergy and above- and below-ground sink competition in horticultural crops.

Of the 206 Rhizoctonia spp isolates obtained from 135 soil samples collected from different fields in the North and South Islands of New Zealand 55 were pathogenic on radish (Raphanus sativus cv Rex) seedlings Only 27 of the isolates that were strongly pathogenic on radish were also pathogenic on ryegrass (Lolium perenne) While 13 of the 92 hypovirulent isolates provided gt;50 protection to radish seedlings against dampingoff caused by Rhizoctonia solani in a screening experiment only three provided gt;50 protection in the final more detailed experiment The best protective isolates R8510 and R308 consistently protected approximately 70 of radish seedlings in at least two separate experiments There was no correlation between the growth rates of the hypovirulent Rhizoctonia spp isolates and their percentage protection of radish seedlings against dampingoff

Recent developments on environmental fate models indicate that as nano waste, engineered nanomaterials/nanoparticles (ENM/Ps) could reach terrestrial ecosystems thus potentially affecting environmental and human health. Plants can be therefore exposed to ENM/Ps but controversial data in terms of fate and toxicity are currently available. Furthermore, there is a current lack of information on complex interactions/transformations to which ENM/Ps undergo in the natural environment as for instance with existing toxic compounds. The main aim of current study is to evaluate potential toxicological risks due to the exposure of plants to ENM/Ps in their natural environment, and investigating different routes of exposure (i.e. water and soil). The aim of the first study reported in chapter 1 was to asses behavior and biological effects of titanium dioxide nanoparticles (n- TiO2) (Aeroxide P25, Degussa Evonik) and its interaction with cadmium (CdCl2) in plants using radish seeds (Raphanus sativus parvus) as model species. Radish seeds were exposed to different concentrations of n-TiO2 (range 1-1000 mg/L) and CdCl2 ( range 1-250 mg/L) alone and in combination using a seed germination and seedling growth toxicity test OECD 208. Percentages of seed germination, germination index (GI) and root elongation were calculated. Cell morphology and oxidative stress parameters as glutathione-S-transferase (GST) and catalase activities (CAT) were measured in radish seeds after 5 days of exposure. Z-Average, PdI and Z-potential of n-TiO2 in Milli-Q water as exposure medium were also determined. DLS analysis showed small aggregates of n-TiO2, negative Z-potential and stable PdI in seed’s exposure media. Germination percentage, GI and root length resulted affected by n-TiO2 exposure compared to controls. Exposure of CdCl2 significantly abolished germination % and GI compared to control seeds and a concentration dependent decrease on root elongation was observed against controls (p<0.05). As well, significant decrease of germination %, GI and root elongation was observed in seeds co-exposed to n-TiO2 and CdCl2 at the highest concentrations (1000mg/L n-TiO2 and 250 mg/L CdCl2) compared to co-exposed seeds at low concentration (1mg/L n-TiO2 and 1 mg/L CdCl2) and controls (p<0.05). Root elongation significantly increase compared to controls at the lowest co-exposure concentration (p<0.05). Similarly at intermediate concentrations of 10 and 100 mg/L in co-exposure conditions, n-TiO2 did not affect CdCl2 toxicity. Concerning antioxidant enzymes, a significant increase of CAT activity in seeds exposed to single high n-TiO2 concentration (1000 mg/L) was observed while n-TiO2 (1 mg/ L), CdCl2 (1 and 250 mg/L) and co-exposure resulted significantly decreased compared to controls (p<0.05). Regarding GST activity, a slight increase in seeds exposed to 1000 mg/L n-TiO2 but no significantly was observed, however both n-TiO2 and CdCl2 alone (1 and 250 mg/L, respectively) or in combinations caused a significant decrease in GST activity (p<0.05). Therefore, overall data support the hypothesis that the presence of n-TiO2 do not affect the toxicity of CdCl2 at least at the highest concentration (100 and 250 mg/L) in radish seeds. Morphological alterations in nuclei, vacuoles and shape of radish root cells were observed upon single Cd exposure and not abolished in the presence of n-TiO2. Nevertheless, although n-TiO2 seems not to reduce Cd toxicity at high concentration (up to 250 mg/L), interactions cannot be excluded based on obtained results. The aim of the second study reported in chapter 2 was to assess if the presence of n-TiO2 might affect elutriate toxicity to radish seeds (R. sativus parvus) seeds as a model species. Radish seeds were exposed to 11 soils (elutriates) alone and in combination with 1 mg/L of n-TiO2 collected from an industrial site located in Taranto area (South East of Italy). Exposure of seeds was performed according to OECD (208) guideline. Then, root elongation, percentages of seed germination and germination index% (GI) were analyzed. In addition, levels of several trace elements were also determined in soils in order to assess their level of contamination and effects on root elongation, seed germination and GI% further discussed. Main results revealed that the presence of n-TiO2 seems not affecting root length, GI % and germination% of seeds compared to seeds exposed to elutriates alone with the exception of only 2 sites. Moreover, the absence of any clear relationship between effects of elutriate on radish seed germination and trace elements levels was observed. Only slight but not significant changes based on levels of trace elements present in soil were observed in growth parameters. In particular levels of Co, Ni, Zn, Cu, Ti and Sn seem to affect radish seeds germination more than others. Regarding co-exposed seeds, the presence of n-TiO2 caused 100% of germination of radish seeds. Furthermore, in comparison to exposed seeds to elutriates alone, root length and GI % resulted more stimulated. Only slight effects on GI% and root length were observed which might be linked to interaction of these elements with n-TiO2. Likewise, it seemed that Co, Se, Sb and As in presence of n-TiO2 are responsible for changes on growth parameters. According on the overall results, soil elutriates alone could not be able to show real toxicity of a contaminated soil on seeds germination and future study should be performed in order to assess their suitability in real exposure scenarios. Therefore, based on observed data further investigations are required in order to assess real environmental scenarios where such particles could be present in soils together with existing contaminants such trace elements. The purpose of third study reported in chapter 3 chapter was to assess the impact of n-TiO2 alone and in combination with CdCl2 on germination and growth of radish seeds (R. sativus) exposed in vitro (experiment 1) and in vivo (directly into soils) (experiment 2). In experiment 1(in vitro) radish seeds were exposed to n-TiO2 (1 and 1000 mg/L and CdCl2) and CdCl2 (1 and 250 mg/L) alone and in combination (n-TiO2 1, 10, 100, 1000 mg/L and CdCl2 1, 10, 100, 250 mg/L) using a seed germination and seedling growth toxicity test OECD 208. In experiment 2 (in vivo), radish exposed only to water and then seedling transferred to soils contaminated with n-TiO2 (1 and 1000 mg/L and CdCl2) and CdCl2 (1 and 250 mg/L) alone and in combination (n-TiO2 1, 10, 100, 1000 mg/L and CdCl2 1, 10, 100, 250 mg/L), still following OECD 208 test conditions. Root length, shoot length and numbers of secondary leaves of all plants from the two experiments (1 and 2) were recorded at day 10 and day 21. Growth parameters of radish at both day 10 and day 21 showed that plants from seeds exposed during germination (experiment 1) resulted more affected by single and co-exposure to n-TiO2 and CdCl2 than those exposed directly in soil (experiment 2). Furthermore, presence of CdCl2 at 250 mg/L alone and in combination with 1000 mg/L of n-TiO2 seemed affect the root and shoot length in both experiments 1and 2 at day 10 and day 21. Growth parameter analysis of single and co-exposed groups in experiment 1 at day 10, showed a decrease in root length in all tested plants with exception of those exposed to n-TiO2 (1mg/L), co-exposed to n-TiO2 and CdCl2 (1mg/L and 1mg/L), (10 mg/L and 10 mg/L) and (100 mg/L and 10 mg/L) which showed slight increase compared to control. In experiment 2 at day10 only exposed plants to 1000 mg/kg of n-TiO2 revealed significant increase of root length while other all single and co-exposure groups showed a decrease of root length respect to control. Shoot length in exposed plants to all single and co-exposure groups in both experiments 1 and 2 at day 10 showed a decrease compared to control except plants exposed to n-TiO2 and CdCl2 (100 mg/kg and 10 mg/kg) in experiment 2 which showed an increase. Obtained results on day 21 showed a decrease of root length respect to control on tested plants to all single and co-exposure groups in both experiments 1 and 2 with exception of exposed radish to 1 mg/L of CdCl2 in experiment 1. Shoot length of all tested single and co-exposure groups in experiments 1 and 2 showed a decrease compared to control except radish exposed to 1000 mg/kg of n-TiO2 which revealed an increase in experiment 2. Regarding secondary leaves, in both experiments 1and 2 at day 10 no leaves were shown. On the opposite, (2 leaves) were present at day 21 in most plants exposed to single and in combination, while those exposed to CdCl2 (250 mg/kg), n-TiO2 and CdCl2 (10 and 100 mg/L) and (1000 mg/kg and 250 mg/kg) in experiment 1 showed no leaves. Likewise, exposure n-TiO2 (1000 mg/kg) and co-exposure of n-TiO2 and CdCl2 (10 mg/kg and 100) showed only one secondary leaf in experiment 2 at day 21.

In tropical, subtropical, and temperate climates, radish (Raphanus sativus L.) is a popular root vegetable. Radish diversity is intense from the eastern Mediterranean to the Caspian Sea. Many radish varieties have varied leaf morphology, root color, size, shape, flavor, vernalization requirements, and maturity times. Early radish variants were long and tapered rather than cylindrical, bulbous, elliptic, or spherical. For black Spanish radish, European-cultivated variety, and Asian-cultivated radish, three separate domestication processes occurred. The original radishes were black, followed by white in the 1500s then red and round in the 1700s. These are R. sativus L. var. radicula (sativus) or R. sativus L. var. niger radishes. Because of protogyny, self-incompatibility, open architecture, and biennial bolting, radish crosses readily. The fundamental methods for using heterotic breeding potential are SI, CMS, and doubled haploids (DH). This chapter discusses the various breeding strategies like inbred line development by the use of self-incompatibility, hybrid development by using male sterility system, population improvement, mutation breeding, haploid breeding, breeding strategies for biotic and abiotic stresses, QTL mapping, and genome wide and genomic tool in radish. Rapid developments in our understanding of advanced biotechnology technologies will increase our ability to identify cultivars and parental lines, check seed genetic purity, analyze phylogenetic links and genetic diversity, and add specific transgenic traits.

Although research has been conducted on intergeneric hybridization between Brassica and Raphanus, much of it remains unpublished. We have acquired numerous Brassica rapa ssp. pekinensis (kimchi cabbage) and R. sativus var. major (“big root radish”) hybrids, originally classified as intergeneric hybrids and named “baechumu” in 1995. A cultivar was identified BB#12, (renamed BB#1 for registration) in baemoochae following stabilization via a microspore mutation in 2006. Numerous hybrids were created for various purposes; some were sterile when self-pollinated but fertile in crosses with other cultivars. Microspore mutation also produced, BB#12x is a novel intergeneric hybrid. A new stable plant variety, BB#5, was selected from numerous inbred lines and produced via microspore culture; it has a very late bolting time and is cultivated in spring. The cultivar purple BB#10 was developed by adding radish chromosomes to turnip, including one providing the purple color, and double-crossing with BB#12, CMS BB#12, and normal BB#12. Now that the hybrid between ssp. pekinensis and radish has produced mature seeds as a dominant property, intergeneric hybrid cultivars can be bred in the future.