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Littérature scientifique sur le sujet « RT-PCR (Reverse Transcription Polymerase Chain Reaction) »

Auteur : Grafiati

Publié le 2 juin 2025

Mis à jour le 31 juillet 2025

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Articles de revues sur le sujet "RT-PCR (Reverse Transcription Polymerase Chain Reaction)"

Romero, JoséR. "Reverse-Transcription Polymerase Chain Reaction Detection of the Enteroviruses." Archives of Pathology & Laboratory Medicine 123, no. 12 (1999): 1161–69. http://dx.doi.org/10.5858/1999-123-1161-rtpcrd.

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Abstract Objective.—This review focuses on commercial and in-house–developed reverse-transcription polymerase chain reaction (RT-PCR) assays used for the detection of enteroviral infections. In addition to providing details on the performance of RT-PCR, its specificity, and sensitivity, the clinical utility of this diagnostic method with specific reference to its impact on hospitalization and cost savings is addressed. Data Sources.—MEDLINE was searched for reports relating to RT-PCR detection of the enteroviruses in adults and children. The search was restricted to studies reported in English

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Smirnova, Evgeniya V., Konstantin A. Blagodatskikh, Ekaterina V. Barsova, Dmitriy A. Varlamov, Vladimir M. Kramarov, and Konstantin B. Ignatov. "Comparison of Commercially Available Thermostable DNA Polymerases with Reverse Transcriptase Activity in Coupled Reverse Transcription Polymerase Chain Reaction Assays." Methods and Protocols 8, no. 1 (2025): 11. https://doi.org/10.3390/mps8010011.

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Reverse transcription polymerase chain reaction (RT-PCR) is an important tool for the detection of target RNA molecules and the assay of RNA pathogens. Coupled RT-PCR is performed with an enzyme mixture containing a reverse transcriptase and a thermostable DNA polymerase. To date, several biotechnological companies offer artificial thermostable DNA polymerases with a built-in reverse transcriptase activity for use in the coupled RT-PCR instead of the enzyme mixtures. Here, we compared the artificial DNA polymerases and conventional enzyme mixtures for the RT-PCR by performing end-point and rea

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Santos, Carlos Ferreira dos, Vivien Thiemy Sakai, Maria Aparecida de Andrade Moreira Machado, Daniela Nicole Schippers, and Andrew Seth Greene. "Reverse transcription and polymerase chain reaction: principles and applications in dentistry." Journal of Applied Oral Science 12, no. 1 (2004): 1–11. http://dx.doi.org/10.1590/s1678-77572004000100002.

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Various molecular biology techniques have become available in the last few years. One of the most revolutionary of these techniques regarding nucleic acid analysis is the polymerase chain reaction (PCR), which was first described in 1985. This method relies on the exponential amplification of specific DNA fragments, resulting in millions of copies that can serve as templates for different kinds of analyses. PCR can be preceded by a reverse transcription (RT) reaction in order to produce cDNA from RNA (RT-PCR). RT-PCR provides the possibility to assess gene transcription in cells or tissues. PC

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Cross, N. C. P., Feng Lin, and J. M. Goldman. "APPROPRIATE CONTROLS FOR REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION (RT-PCR)." British Journal of Haematology 87, no. 1 (1994): 218. http://dx.doi.org/10.1111/j.1365-2141.1994.tb04899.x.

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Yang, Shuixian, Kai-Yu Wang, and Zexiao Yang. "Comparison of reverse-transcription loop-mediated isothermal amplification and reverse-transcription polymerase chain reaction for grass carp reovirus." Acta Veterinaria Brno 84, no. 3 (2015): 215–23. http://dx.doi.org/10.2754/avb201584030215.

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Grass carp reovirus (GCRV) has been assigned to a newly established Aquareovirus genus in the family of Reoviridae which leads to haemorrhagic disease and extremely high mortality rate in grass carp. In this study, comparison was made between the novel one-step reverse transcription loop-mediated isothermal amplification (RT-LAMP) and the reverse transcription polymerase chain reaction (RT-PCR) for detection of grass carp reovirus. The result indicated that RT-LAMP had × 10 higher sensitivity comparable to RT-PCR. The specificity of the two methods for GCRV detection were both developed succes

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Paramata, Nanang Roswita, Winansih Gubali, and Rahma Yulia Adipu. "Results of Reverse Transcription-Polymerase Chain Reaction (RT-PCR) Examination in Covid-19 Patients at Prof. Dr. H. Aloei Saboe City of Gorontalo." Jambura Nursing Journal 5, no. 1 (2023): 86–93. http://dx.doi.org/10.37311/jnj.v5i1.18323.

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Coronavirus Disease or COVID-19 is still a concern throughout the world. WHO recommends the Reverse Transcription-Polymerase Chain Reaction (RT-PCR) method as the gold standard for diagnosing COVID-19. The purpose of this study was to describe the results of the Reverse Transcription-Polymerase Chain Reaction (RT-PCR) examination in COVID-19 patients at Prof. Dr. H. Aloe Saboe, City of Gorontalo. This research is a type of descriptive qualitative research with a retrospective approach. The population of this study were all suspected and confirmed COVID-19 patients treated at Prof. Hospital. Dr

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Wang, Gehua, Connie Barton, and Frank G. Rodgers. "Bacterial DNA decontamination for reverse transcription polymerase chain reaction (RT-PCR)." Journal of Microbiological Methods 51, no. 1 (2002): 119–21. http://dx.doi.org/10.1016/s0167-7012(02)00041-6.

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Bustin, SA. "Absolute quantification of mRNA using real-time reverse transcription polymerase chain reaction assays." Journal of Molecular Endocrinology 25, no. 2 (2000): 169–93. http://dx.doi.org/10.1677/jme.0.0250169.

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The reverse transcription polymerase chain reaction (RT-PCR) is the most sensitive method for the detection of low-abundance mRNA, often obtained from limited tissue samples. However, it is a complex technique, there are substantial problems associated with its true sensitivity, reproducibility and specificity and, as a quantitative method, it suffers from the problems inherent in PCR. The recent introduction of fluorescence-based kinetic RT-PCR procedures significantly simplifies the process of producing reproducible quantification of mRNAs and promises to overcome these limitations. Neverthe

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Yoo, Hee-Min, Il-Hwan Kim, and Seil Kim. "Nucleic Acid Testing of SARS-CoV-2." International Journal of Molecular Sciences 22, no. 11 (2021): 6150. http://dx.doi.org/10.3390/ijms22116150.

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The coronavirus disease 2019 (COVID-19) has caused a large global outbreak. It is accordingly important to develop accurate and rapid diagnostic methods. The polymerase chain reaction (PCR)-based method including reverse transcription-polymerase chain reaction (RT-PCR) is the most widely used assay for the detection of SARS-CoV-2 RNA. Along with the RT-PCR method, digital PCR has emerged as a powerful tool to quantify nucleic acid of the virus with high accuracy and sensitivity. Non-PCR based techniques such as reverse transcription loop-mediated isothermal amplification (RT-LAMP) and reverse

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Al-Shamary, Abd Al-Hakeem A. "SEROLOGICAL AND MOLECULAR DETECTION OF COWPAE MOSAIC VIRUS INFECTING COWPAE IN IRAQ." Diyala Agricultural Sciences Journal 11, no. 2 (2019): 9–17. http://dx.doi.org/10.52951/dasj.19110202.

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This study was conducted to detect CPMV in infecting cowpea plantes at Plant Protection Department/College of Agriculture, University of Baghdad. Symptomatic cowpea plants collected from fields in Baghdad and Diyala Provinces were tested by Emzyme-linked immunosorbent assay (ELISA), reverse transcription- polymerase chain reaction RT-PCR and Immuno-capture reverse transcription-polymerase chain reaction (IC-RT-PCR) using commercial kits. ELISA approach could detect Cowpea mosaic virus CpMV in tested samples but with virus concentration lower than the positive control supplied with the kit, ind

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Thèses sur le sujet "RT-PCR (Reverse Transcription Polymerase Chain Reaction)"

Saunders, Daniel Curtis. "Microfluidic system with open loop control for rapid infrared reverse transcription quantitative PCR (RT-qPCR)." Thesis, Georgia Institute of Technology, 2012. http://hdl.handle.net/1853/44865.

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Microfluidic techniques have allowed for fast, sensitive, and low cost applications of the Polymerase Chain Reaction (PCR) through the use of small reaction volumes, rapid amplification speeds, and the on-chip integration of upstream and downstream sample handling processes including purification and electrophoretic separation functionality. While such systems are capable of measuring the expression levels of thousands of genes simultaneously, or in hundreds of cells, or with sample-in and answer-out capability, none of these systems are easily scalable in the time domain. Because of this, t

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Lekas, Poli. "Analysis of human CYP2E1 mRNA in a HepG2 cell line by reverse transcription-polymerase chain reaction (RT-PCR)." Thesis, National Library of Canada = Bibliothèque nationale du Canada, 1998. http://www.collectionscanada.ca/obj/s4/f2/dsk1/tape11/PQDD_0014/MQ40842.pdf.

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Zhang, Tieling 1968. "Gene expression analysis of the proteinase inhibitor gene PiA of potato (Solanum tuberosum) by reverse transcription polymerase chain reaction (RT-PCR)." Thesis, McGill University, 2002. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=79212.

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In this study the gene expression pattern of a novel proteinase inhibitor II (PI-II) gene, PiA, in potato (Solanum tuberosum ), was analyzed using RT-PCR. The PiA gene was found to have enhanced expression in expanding tubers of a greenhouse-grown diploid clone (DC), different stages of 'Russet Burbank' tubers and expanding microtubers of in vitro-grown 'Russet Burbank' and 'Bintje'. PiA mRNA was more abundant in the tuber vascular ring and perimedullary zone areas than in other tuber tissues of DC. The PiA gene was also expressed in flower buds/flowers, shoot apices, leaf blades, stems

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Bontoux, Nathalie. "Analyse du transcriptome d'une cellule unique à l'aide d'une puce microfluidique." Paris 6, 2006. http://www.theses.fr/2006PA066600.

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Kwan, See-wai Grace. "Detection of human coronavirus infections by reverse transcription PCR in children hospitalized with respiratory disease in Hong Kong /." View the Table of Contents & Abstract, 2005. http://sunzi.lib.hku.hk/hkuto/record/B31494274.

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Wires, Shannon Meredith. "Rapid detection of Norwalk-like viruses by reverse transcription-polymerase chain reaction (RT-PCR) and southern blot hybridisation (SBH) in outbreaks of acute gastroenteritis in eastern Ontario, Canada." Thesis, National Library of Canada = Bibliothèque nationale du Canada, 2001. http://www.collectionscanada.ca/obj/s4/f2/dsk3/ftp04/MQ59412.pdf.

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Chehtane, Mounir. "REAL TIME REVERSE TRANSCRIPTION-POLYMERASE CHAIN REACTION FOR DIRECT DETECTION OF VIABLE MYCOBACTERIUM AVIUM SUBSPECIES PARATUBERCULOSIS IN CROHN S DISEASE PATIENTS and ASSOCIATION OF MAP INFECTION WITH DOWNREGUALTION IN INTERFERON-GAMMA RECEPTOR (INFG." Master's thesis, University of Central Florida, 2005. http://digital.library.ucf.edu/cdm/ref/collection/ETD/id/4281.

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Association of Mycobacterium avium subspecies paratuberculosis (MAP) with Crohn's disease (CD) and not with ulcerative colitis (UC), two forms of inflammatory bowel disease (IBD), has been vigorously debated in recent years. This theory has been strengthened by recent culture of MAP from breast milk, intestinal tissue and Blood from patients with active Crohn's disease. Culture of MAP from clinical samples remained challenging due to the fastidious nature of MAP including its lack of cell wall in infected patients. The advent of real time PCR has proven to be significant in infectious disease

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Lam, Siu-yan. "Multiplex reverse transcription-PCR for detection and identification of human parainfluenza viruses 1,2,3 and 4 infection in hospitalized children with respiratory disease in Hong Kong /." View the Table of Contents & Abstract, 2007. http://sunzi.lib.hku.hk/hkuto/record/B3848058X.

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Chan, Kit-man, and 陳潔雯. "Detection of human enteroviruses by reverse transcription-PCR in hospitalized children with respiratory disease in Hong Kong." Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 2010. http://hub.hku.hk/bib/B44669963.

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Lassauniere, Maria Magdalena. "Development of quantitative multiplex real-time RT-PCR assays for detection of 13 conventional and newly discovered viruses associated with lower respiratory tract infections in children in South Africa." Diss., University of Pretoria, 2010. http://hdl.handle.net/2263/28457.

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Acute lower respiratory tract infection (ALRI) is a major cause of paediatric morbidity and mortality, annually accounting for approximately 2 million childhood deaths worldwide of which up to 90% resides in the developing world. In 12-39% of ALRI cases no aetiological agent is identified, despite comprehensive investigations, thus suggesting that additional unknown agents may be involved. Since 2001 a number of new viruses have been identified that may account for some of these cases including human metapneumovirus (hMPV), human bocavirus (hBoV), and two new coronaviruses (hCoV) NL63 and HKU1

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Livres sur le sujet "RT-PCR (Reverse Transcription Polymerase Chain Reaction)"

Colonna-Romano, Sergio. Differential-display reverse transcription-PCR (DDRT-PCR). Springer-Verlag, 1998.

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Lekas, Poli. Analysis of human CYP2E1 mRNA in a HepG2 cell line by reverse transcription-polymerase chain reaction (RT-PCR). National Library of Canada, 1998.

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Mireille, Raccurt, ed. PCR/RT-PCR in situ light and electron microscopy. CRC Press, 2003.

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RT-PCR protocols. 2nd ed. Humana, 2010.

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Morel, Gerard, and Mireille Raccurt. PCR/RT- PCR in situ: Light and Electron Microscopy (Methods in Visualization). CRC, 2002.

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Chapitres de livres sur le sujet "RT-PCR (Reverse Transcription Polymerase Chain Reaction)"

Donaldson, Lucy F. "Polymerase Chain Reaction (PCR) and Reverse Transcription (RT)-PCR." In Essential Guide to Reading Biomedical Papers. John Wiley & Sons, Ltd, 2012. http://dx.doi.org/10.1002/9781118402184.ch20.

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Iralu, Nulevino, Dasari Meghanath, Sumiah Wani, Shahjahan Rashid, Wani Farhana, and Aflaq Hamid. "Reverse Transcription Polymerase Chain Reaction (RT-PCR) for RNA Viruses." In Springer Protocols Handbooks. Springer US, 2025. https://doi.org/10.1007/978-1-0716-4390-7_21.

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Roy, Subhamoy Singha, and Mallika Aich. "Reverse Transcription Polymerase Spin Chain Reaction (RT-PSCR)." In Springer Proceedings in Mathematics & Statistics. Springer Nature Singapore, 2025. https://doi.org/10.1007/978-981-96-3098-1_5.

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King, Nicola. "Measurement of Cardiac Gene Expression by Reverse Transcription Polymerase Chain Reaction (RT-PCR)." In Methods in Molecular Biology. Humana Press, 2007. http://dx.doi.org/10.1007/978-1-59745-030-0_6.

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Cale, Jacqueline M., Cynthia E. Shaw, and Ian M. Bird. "Optimization of a Reverse Transcription-Polymerase Chain Reaction (RT-PCR) Mass Assay for Low-Abundance mRNA." In Phospholipid Signaling Protocols. Humana Press, 1998. http://dx.doi.org/10.1385/0-89603-491-7:351.

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Malinowski, Tadeusz. "Silicacapture-Reverse Transcription-Polymerase Chain Reaction (SC-RT-PCR): Application for the Detection of Several Plant Viruses." In Developments in Plant Pathology. Springer Netherlands, 1997. http://dx.doi.org/10.1007/978-94-009-0043-1_97.

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Caballero-Solares, Albert, Jennifer R. Hall, Xi Xue, and Matthew L. Rise. "Reverse Transcription-Quantitative Real-Time Polymerase Chain Reaction (RT-qPCR) for Gene Expression Analyses." In Methods in Molecular Biology. Springer US, 2022. http://dx.doi.org/10.1007/978-1-0716-2376-3_21.

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Loughney, Andrew D., and Christopher P. F. Redfern. "Reverse Transcriptase-Polymerase Chain Reaction (RT-PCR) for Cellular Retinoid-Binding Proteins." In Retinoid Protocols. Humana Press, 1998. http://dx.doi.org/10.1385/0-89603-438-0:81.

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Yao, Kai-Ling, Mary Josephine Pilat, and Kenneth J. Pienta. "Reverse transcriptase- polymerase chain reaction (RT-PCR) to detect prostate cancer micrometastasis in the blood." In Diagnosis and Treatment of Genitourinary Malignancies. Springer US, 1996. http://dx.doi.org/10.1007/978-1-4615-6343-3_4.

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O’Driscoll, Lorraine, Carmel Daly, Mohamad Saleh, and Martin Clynes. "The use of reverse transcriptase-polymerase chain reaction (RT-PCR) to investigate specific gene expression in multidrug-resistant cells." In Multiple Drug Resistance in Cancer. Springer Netherlands, 1994. http://dx.doi.org/10.1007/978-94-011-0826-3_14.

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Actes de conférences sur le sujet "RT-PCR (Reverse Transcription Polymerase Chain Reaction)"

Le Borgne, S., J. M. Romero, H. A. Videla, J. M. Gonzalez, and C. Saiz-Jiménez. "Practical Cases of the Use of Molecular Techniques to Characterize Microbial Deterioration of Metallic Structures in Industry." In CORROSION 2007. NACE International, 2007. https://doi.org/10.5006/c2007-07523.

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Abstract Two specific cases of applying molecular techniques for deciphering the role of microorganisms in industrial processes are presented: an offshore seawater injection system and a wastewater treatment plant. In the first case, deoxyribonucleic acid (DNA) was extracted from a water sample taken from an offshore seawater injection system and from enrichment cultures from the same sample. The V3 hypervariable region of the 16S rDNA gene was amplified by the polymerase chain reaction (PCR) and bacterial diversity was studied using denaturing gel gradient electrophoresis (DGGE). DGGE monitor

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Shirobokova, S. A., A. V. Shabalina, I. S. Sukhikh, A. S. Dolgova, and V. G. Dedkov. "DEVELOPMENT OF A REAL-TIME RT-PCR METHOD FOR DETECTING SABIA VIRUS RNA." In XI МЕЖДУНАРОДНАЯ КОНФЕРЕНЦИЯ МОЛОДЫХ УЧЕНЫХ: БИОИНФОРМАТИКОВ, БИОТЕХНОЛОГОВ, БИОФИЗИКОВ, ВИРУСОЛОГОВ, МОЛЕКУЛЯРНЫХ БИОЛОГОВ И СПЕЦИАЛИСТОВ ФУНДАМЕНТАЛЬНОЙ МЕДИЦИНЫ. IPC NSU, 2024. https://doi.org/10.25205/978-5-4437-1691-6-206.

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Sabia virus (Mammarenavirus brazilense) is the causative agent of Brazilian hemorrhagic fever, the least studied of the fevers caused by arenaviruses. A one-step qualitative reverse transcription polymerase chain reaction assay was developed to detect Sabia virus RNA in biological samples. The detection limit of the assay was 103 copies/ml.

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Jevremović, Darko, Bojana Vasilijević, Tatjana Anđelić, and Tatjana Vujović. "APPLICATION OF QPCR FOR PLUM POX VIRUS DETECTION DURING CRYOTHERAPY." In 1st International Symposium on Biotechnology. University of Kragujevac, Faculty of Agronomy, 2023. http://dx.doi.org/10.46793/sbt28.283j.

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For the purpose of removing viruses from infected plant material, cryotherapy is a novel application of the plant cryopreservation technique. The use of various cryotherapy procedures and treatments necessitates the examination of cryo-treated material for the presence of the targeted pathogen in order to gauge the effectiveness of the therapy. In our study, we evaluated the efficiency of reverse transcription-polymerase chain reaction (RT-PCR) and qPCR (quantitative PCR) methods for the detection of plum pox virus (PPV) in cryo-treated material of plums ‘Belošljiva’ and ‘Crvena Ranka’. A qRCR

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Vasilijević, Bojana, and Darko Jevremović. "Occurrence of Rubus yellow net virus in red raspberry orchards in Serbia." In 7th International Scientific Conference Modern Trends in Agricultural Production, Rural Development and Environmental Protection. The Balkans Scientific Center of the Russian Academy of Natural Sciences, 2025. https://doi.org/10.46793/7thmtagricult.08v.

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A survey for Rubus yellow net virus (RYNV) in red raspberry orchards in Serbia was conducted from 2014‒2022. A total of 112 samples from seven cultivars were collected and analyzed. Reverse transcription polymerase chain reaction (RT-PCR) using RYNV-specific primers was used for virus detection. A specific 350 bp fragment was obtained in 30 samples, confirming RYNV presence in cultivars ‘Fertödi Zamatos’, ‘Meeker’, ‘Tulameen’, and ‘Willamette’. Sequence analysis of the partial polyprotein gene of the RYNV genome confirmed that selected Serbian isolates share 83.43–98.29% nucleotide identities

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Fleming, Paul, and Tara Dalton. "One-Step Reverse-Transcription PCR on a High-Throughput Micro-Fluidic Device." In ASME 2009 Summer Bioengineering Conference. American Society of Mechanical Engineers, 2009. http://dx.doi.org/10.1115/sbc2009-206623.

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One step reverse-transcription polymerase chain reaction (RT-PCR) assays are an attractive option for further automating gene detection assays. One-step assays can reduce hands–on-time and the risk of sample crossover and contamination. The one-step chemistries are showing increasing use in virus detection and have been reported, in some cases, to be more appropriate than their two-step counterparts [1, 2]. Previous work presented by the Stokes Institute research group outlined a micro fluidic based continuous flow instrument which performed high throughput qPCR in nanolitre sized droplets [3]

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Go, Russelle, Lee Romasanta, Pamela Faye Daza, Darel Alexie David, Luieza Sabello, and Michael Nayat Young. "A Study on COVIDBIT as a COVID-19 Detection Device for an Alternative Reverse Transcription-Polymerase Chain Reaction (RT-PCR) Machine." In ICBEA 2022: 2022 6th International Conference on Biomedical Engineering and Applications. ACM, 2022. http://dx.doi.org/10.1145/3543081.3543101.

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Stoicescu, Ramona, Razvan-Alexandru Stoicescu, Codrin Gheorghe, Adina Honcea, and Iulian Bratu. "CONSIDERATIONS ON SARS-COV-2 DIAGNOSIS IN THE LABORATORY OF UNIVERSITY EMERGENCY CLINICAL HOSPITAL OF CONSTANTA." In GEOLINKS Conference Proceedings. Saima Consult Ltd, 2021. http://dx.doi.org/10.32008/geolinks2021/b1/v3/07.

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Coronaviruses are members of the Coronaviridae family. They are enveloped, non-segmented, positive-sense, single-stranded RNA viruses. Their genome size is about 30 kilobases (kb) which consist, at the 5’ end, of non-structural open reading frames (ORFs: ORF1a, ORF 1b) which code for 16 non structural proteins, and at the 3’ end the genes which code for four structural proteins including membrane (M), envelope (E), spike (S), and nucleocapsid (N) proteins. Due to the rapid spread of COVID-19, a reliable detection method is needed for patient diagnosis especially in the early stages of the dise

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Shikata, Tetsuo, Toshihiko Shiraishi, Kumiko Tanaka, Shin Morishita, and Ryohei Takeuchi. "Effects of Acceleration Amplitude and Frequency of Mechanical Vibration on Osteoblast-Like Cells." In ASME 2007 International Mechanical Engineering Congress and Exposition. ASMEDC, 2007. http://dx.doi.org/10.1115/imece2007-41797.

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Bone formation is subject in vivo to mechanical stimulation. Although many researches for bone cells of osteoblastic lineage sensing and responding to mechanical stimulation have been reported mainly in the biochemical field, effects of mechanical stimulation on bone cells are not well understood. In this study, in order to clarify effects of acceleration amplitude and frequency of mechanical stimulation on MC3T3-E1, which is an osteoblast-like cell line derived from mouse calvaria, in the sense of mechanical vibrations, their cell proliferation, cell morphology, bone matrix generation and gen

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Ahmad, Salma, Hanan Nazar, Nouralhuda Alatieh, et al. "Validation of Novel Transcriptional Targets that Underpin CD44-promoted breast cancer cell invasion." In Qatar University Annual Research Forum & Exhibition. Qatar University Press, 2021. http://dx.doi.org/10.29117/quarfe.2021.0153.

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Introduction: Breast cancer (BC) is the most common cancer worldwide, and metastasis is its worst aspect and the first cause of death. Metastasis is a multistep process, where an invasion is a recurring event. The process of BC cell invasion involves three major factors, including cell adhesion molecules (CAM), proteinases and Growth factors.CD44, a family of CAM proteins and the hyaluronic acid (HA) cell surface receptor, acts as cell differentiation, cell migration/invasion and apoptosis regulator. Rationale: We have previously established a tetracycline (Tet)-OFF-regulated expression system

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ML, Liji, and Escalin Freetha T. "Edge-Cloud Collaboration Based Covid-19 Detection on X-Ray Images." In 7th International Conference on Recent Innovations in Computer and Communication (ICRICC 23). International Journal of Advanced Trends in Engineering and Management, 2023. http://dx.doi.org/10.59544/qyok1917/icricc23p19.

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The Corona virus disease 2019 (COVID19) pandemic has led to a dramatic loss of human life worldwide and caused a tremendous challenge to public health. Immediate detection and diagnosis of COVID19 have lifesaving importance for both patients and doctors. The availability of COVID19 tests increased significantly in many countries, thereby provisioning a limited availability of laboratory test kits additionally, the Reverse Transcription Polymerase Chain Reaction (RT PCR) test for the diagnosis of COVID 19 is costly and time consuming. X ray imaging is widely used for the diagnosis of COVID19. T

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Rapports d'organisations sur le sujet "RT-PCR (Reverse Transcription Polymerase Chain Reaction)"

กุลวิชิต, วันล่า. โครงการ การตรวจหาปริมาณและความหนาแน่นของไวรัสเดงกี ที่ตรวจพบในเม็ดเลือดขาวชนิดต่าง ๆ ในเลือดและในซีรั่มหรือพลาสมา ด้วยวิธี RT-PCR (reverse transcription - polymerase chain reaction) และ real-time PCR. คณะแพทยศาสตร์ จุฬาลงกรณ์มหาวิทยาลัย, 2005. https://doi.org/10.58837/chula.res.2005.21.

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โรคติดเชื้อไวรัสเองกี เป็นปัญหาสาธารณสุขที่สำคัญที่สุดอย่างหนึ่งของประเทศไทย การวินิจฉัยการติดเชื้อทางห้องปฏิบัติการ ยังมีข้อจำกัดอยู่ที่ความจำเป็นต้องเจาะเลือก ผู้ป่วยเด็กมักไม่ใครให้ความร่วมมือดีนัก คณะผู้วิจัยได้ทำการศึกษาความเป็นไปได้ในการใช้สิ่งส่งตรวจในช่องปาก และ/หรือปัสสาวะของผู้ป่วย มาทำการตรวจวินิจฉัยทางห้องปฏิบัติการ จากการศึกษาเบื้องต้น ในผู้ป่วยหลายสิบราย พบว่า สามารถตรวจพบเชื้อไวรัสในน้ำลายได้ โดยวิธี reverse transcription polymerase chain reaction (RT-PCR) ประมาณ 30-50% ของผู้ป่วย และตรวจได้ในปัสสาวะประมาณ 80% แม้จะเป็นสิ่งส่งตรวจในระยะท้าย ๆ ของไข้ ซึ่งนับเป็นอัตราการตรวจพบที่ใ

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ศุภปีติพร, กัญญา, วรมันต์ ไวดาบ та วรศักดิ์ โชติเลอศักดิ์. การศึกษาความสัมพันธ์ของระดับการแสดงออกของยีน ที่เกี่ยวข้องกับระบบภูมิคุ้มกันกับความรุนแรงของโรคติดเชื้อไวรัสเด็งกิว : รายงานผลการวิจัย. จุฬาลงกรณ์มหาวิทยาลัย, 2009. https://doi.org/10.58837/chula.res.2009.16.

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ศึกษาความสัมพันธ์ของระดับการแสดงออกของยีน (mRNA) ที่เกี่ยวข้องกับระบบภูมิคุ้มกันกับความรุนแรงของโรคติดเชื้อไวรัสเด็งกิว ผู้เข้าร่วมการวิจัยประกอบด้วยผู้ป่วยที่ได้รับการวินิจฉัยว่าติดเชื้อไวรัสเด็งกิวและได้รับการจำแนกตามรุนแรงของการติดเชื้อ โดยใช้หลักเกณฑ์ขององค์การอนามัยโลก ตัวอย่างเลือดที่นำมาสกัด mRNA และนำมาวิเคราะห์ได้จากผู้ป่วยเด็กที่ติดเชื้อไวรัสเด็งกิวในวันที่ไข้ลง ระดับการแสดงออกของยีน IL-8, IL-1beta และ MMP-9 ในเม็ดเลือดขาวของผู้ป่วยเด็กที่เป็นโรคไข้เด็งกิว (Dengue fever) จำนวน 30 ราย ผู้ป่วยเด็กที่เป็นโรคไข้เลือดออก (Dengue hemorrhagic fever) จำนวน 19 ราย และผู้ป่วยเด็กที่มีไข้จากการ

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โกวิทย์ดำรงค์, เอกสิทธิ์, ภาวพันธ์ ภัทรโกศล, รุจิภัตต์ สำราญสำรวจกิจ та นวลจันทร์ ปราบพาล. อุบัติการณ์ของโรคติดเชื้อไวรัสเฉียบพลันของระบบทางเดินหายใจในเด็กไทย : รายงานการวิจัย. จุฬาลงกรณ์มหาวิทยาลัย, 2009. https://doi.org/10.58837/chula.res.2009.24.

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โรคติดเชื้อเฉียบพลันของระบบทางเดินหายใจส่วนล่างเป็นสาเหตุของการเจ็บป่วยและการเสียชีวิตที่สำคัญในเด็กอายุต่ำกว่า 5 ปีในประเทศที่กำลังพัฒนา สาเหตุส่วนใหญ่เกิดจากการติดเชื้อไวรัส การศึกษานี้ได้ทำการตรวจ nasopharyngeal aspirates ของผู้ป่วยเด็กอายุ 0-5 ปี ที่ป่วยด้วยโรคติดเชื้อเฉียบพลันของระบบทางเดินหายใจส่วนล่างจำนวน 268 ราย ที่เข้ารับการรักษาในโรงพยาบาลจุฬาลงกรณ์ ระหว่างกรกฎาคม พ.ศ. 2550 ถึงกันยายน พ.ศ. 2552 ด้วยวิธี multiplex realtime reverse transcription polymerase chain reaction (RT-PCR) พบว่าร้อยละ 67.2 เกิดจากการติดเชื้อไวรัส โดย respiratory syncytial virus (RSV) เป็นไวรัสที่พบมากที่สุด พบร

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เตียวศิริทรัพย์, สนธยา. บทบาทของยุงพาหะนำโรคและสัตว์เลี้ยงในระบาดวิทยาของเชื้อไวรัสชิคุนกุนยา. จุฬาลงกรณ์มหาวิทยาลัย, 2012. https://doi.org/10.58837/chula.res.2012.65.

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เชื้อไวรัสชิคุนกุนยาเป็นเชื้อที่ก่อให้เกิดอาการเจ็บป่วยในคนโดยที่มียุงเป็นแมลงพาหะนำโรค การศึกษานี้มีวัตถุประสงค์เพื่อศึกษาบทบาทของสัตว์เลี้ยงในระบาดวิทยาของเชื้อไวรัสชิคุนกุนยา โดยทำการศึกษาไวรัสชิคุนกุนยาในกระแสเลือดของสัตว์ปีกและสัตว์เลี้ยงลูกด้วยนม โดยใช้ลูกไก่และหนูไมซ์เป็นสัตว์ทดลองต้นแบบ ทำการฉีดเชื้อไวรัสชิคุนกุนยาสายพันธุ์ที่ระบาดในประเทศไทยในปี พ.ศ. 2553 (Thailand 2010 strain) และสายพันธุ์ที่เคยระบาดในประเทศไทยในอดีตซึ่งเป็นเชื้อมาตรฐานอ้างอิง (Ross/186 strain) ให้กับสัตว์ทดลองในปริมาณที่แตกต่างกัน โดยทำการฉีดเชื้อไวรัสจำนวน 10⁴, 10⁶ และ 10⁸ CID₅₀ ให้กับหนูไมซ์อายุ 4 และ 6 สัปดาห์ หล

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GUI, ZHEN, Yue-Ying WANG, Jia-Xin Li, and Tao XIANG. Prevalence of poor sleep quality in COVID-19 patients: a systematic review and meta-analysis. INPLASY - International Platform of Registered Systematic Review and Meta-analysis Protocols, 2023. http://dx.doi.org/10.37766/inplasy2023.2.0121.

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Review question / Objective: The inclusion criteria for this study are based on the PICOS acronym: Participants (P): COVID-19 patients based on positive Coronavirus RT-PCR (reverse transcription-polymerase chain reaction) of nasopharyngeal and oropharyngeal swabs or a history of COVID-19 infection. Following previous research, the COVID-19 patients in this study will include the period of COVID-19 infection, symptom onset, recovery, and the onset of post-acute COVID-19 symptoms. Interventions (I): not applicable; Comparisons (C): healthy controls in comparative studies, or not applicable to ep

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Siriyasatien, Padet. Effect of double dengue serotypes infection in dengue mosquito (Aedes aegypti). Chulalongkorn University, 2012. https://doi.org/10.58837/chula.res.2012.19.

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We demonstrated the results of laboratory induced dengue virus infection more than 1 serotype in female Aedes aegypti through membrane feeding apparatus. Forty female mosquitoes were allowed to feed human blood contained 4 dengue serotypes at the concentration of 103 pfu/ml. One week after infection, dengue virus were detected by RT-PCR technique. Seven and two mosquitoes were positive for dengue serotype 3 and 4, respectively. Mix infection of dengue virus serotype 3 and 4 was found in 4 female mosquitoes. To determine the infectivity of dengue serotype 1 and 2, both serotypes were used to in

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Pavasant, Prasit, and Tussanee Yongchaitrakul. Influence of mechanical stress on the expression of osteopontin in human periodontal ligament cells. Chulalongkorn University, 2008. https://doi.org/10.58837/chula.res.2008.14.

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Background: Mechanical stress such as orthodontic forces can produce mechanical damage and inflammatory reaction in periodontium. Osteopontin (OPN) is a multifunctional cytokine that found to be correlated with periodontal disease progression. As periodontal ligaments (PDL) can be affected by stress and PDL cells are involved in periodontal destruction and remodeling, we aimed to investigate the influence of mechanical stress on the expression and regulation of OPN in human PDL (HPDL) cells. Methods: The mechanical stress was generated by continuous compressive force and the expression of OPN

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Lertchirakarn, Veera, and Prasit Pavasant. The effects of fluocinolone acetonide on human cultured dental pulp cell in vitro. Chulalongkorn University, 2008. https://doi.org/10.58837/chula.res.2008.15.

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The purpose of treatment of pulpal exposure is to preserve vitality, healthy and promote healing of exposed pulp tissue. Fluocinolone acetonide (FA) may have a potential to promote tissue healing. The aim of this study was therefore to investigate the effects of FA on human cultured dental pulp cell in vitro. The MTT assay was performed to examine both cytotoxicity and prolifration of FA at 24, 48 and 72 hours. The results revealed that FA (0.1 to 50 [mu]M) had no cytotoxicity effect. In addition, these doses also stimulated cell proliferation. There was a significant increase of cell prolifer

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Morrison, Mark, and Joshuah Miron. Molecular-Based Analysis of Cellulose Binding Proteins Involved with Adherence to Cellulose by Ruminococcus albus. United States Department of Agriculture, 2000. http://dx.doi.org/10.32747/2000.7695844.bard.

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At the beginning of this project, it was clear that R. albus adhered tightly to cellulose and its efficient degradation of this polysaccharide was dependent on micromolar concentrations of phenylacetic acid (PAA) and phenylpropionic acid (PPA). The objectives for our research were: i) to identify how many different kinds of cellulose binding proteins are produced by Ruminococcus albus; ii) to isolate and clone the genes encoding some of these proteins from the same bacterium; iii) to determine where these various proteins were located and; iv) quantify the relative importance of these proteins

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วีรกุล, ปราจีน, อัจฉริยา ไศละสูต, คณิศักดิ์ อรวีระกุล, วาสนา ภิญโญชนม์, สุดารัตน์ ดำรงค์วัฒนโภคิน та สันนิภา สุรทัตต์. การวิจัยและการพัฒนาวิธีวินิจฉัย ควบคุมและป้องกันโรคอหิวาต์สุกรในประเทศไทย : รายงานการวิจัย. จุฬาลงกรณ์มหาวิทยาลัย ; สำนักงานคณะกรรมการวิจัยแห่งชาติ, 2002. https://doi.org/10.58837/chula.res.2002.61.

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ชุดโครงการวิจัยนี้ แบ่งการศึกษาออกเป็น 4 โครงการย่อย ซึ่งประกอบด้วย โครงการที่ 1 การศึกษาเปรียบเทียบระดับแอนติบอดีในแม่สุกรที่ได้รับการฉีดวัคซีนโปรแกรม 3 ชนิด และผลกระทบของภูมิคุ้มกันถ่ายทอดจากแม่สู่ลูกในการสร้างภูมิคุ้มกันในลูกสุกรเมื่อได้รับการฉีดวัคซีน โครงการที่ 2 การพัฒนาวิธีการตรวจวินิจฉัยโรคอหิวาต์สุกรและการพัฒนาการตรวจวัดภูมิคุ้มกันชนิดเซลล์ต่อเชื้อไวรัสอหิวาต์สุกร โครงการที่ 3 การศึกษาพัฒนาวิธีผลิตวัคซีนอหิวาต์สุกรชนิดเซลล์เพาะเลี้ยง และโครงการที่ 4 การศึกษาระดับภูมิคุ้มกันถ่ายทอดที่สามารถป้องกันการเกิดอหิวาต์สุกรเมื่อได้รับการฉีดเชื้อพิษอหิวาต์สุกร Chiangmai/98 โครงการที่ 1 การศึกษาส

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