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Articles de revues sur le sujet « RT-PCR (Reverse Transcription Polymerase Chain Reaction) »

Auteur : Grafiati

Publié le 2 juin 2025

Mis à jour le 31 juillet 2025

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1

Romero, JoséR. "Reverse-Transcription Polymerase Chain Reaction Detection of the Enteroviruses." Archives of Pathology & Laboratory Medicine 123, no. 12 (1999): 1161–69. http://dx.doi.org/10.5858/1999-123-1161-rtpcrd.

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Abstract Objective.—This review focuses on commercial and in-house–developed reverse-transcription polymerase chain reaction (RT-PCR) assays used for the detection of enteroviral infections. In addition to providing details on the performance of RT-PCR, its specificity, and sensitivity, the clinical utility of this diagnostic method with specific reference to its impact on hospitalization and cost savings is addressed. Data Sources.—MEDLINE was searched for reports relating to RT-PCR detection of the enteroviruses in adults and children. The search was restricted to studies reported in English

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Smirnova, Evgeniya V., Konstantin A. Blagodatskikh, Ekaterina V. Barsova, Dmitriy A. Varlamov, Vladimir M. Kramarov, and Konstantin B. Ignatov. "Comparison of Commercially Available Thermostable DNA Polymerases with Reverse Transcriptase Activity in Coupled Reverse Transcription Polymerase Chain Reaction Assays." Methods and Protocols 8, no. 1 (2025): 11. https://doi.org/10.3390/mps8010011.

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Reverse transcription polymerase chain reaction (RT-PCR) is an important tool for the detection of target RNA molecules and the assay of RNA pathogens. Coupled RT-PCR is performed with an enzyme mixture containing a reverse transcriptase and a thermostable DNA polymerase. To date, several biotechnological companies offer artificial thermostable DNA polymerases with a built-in reverse transcriptase activity for use in the coupled RT-PCR instead of the enzyme mixtures. Here, we compared the artificial DNA polymerases and conventional enzyme mixtures for the RT-PCR by performing end-point and rea

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Santos, Carlos Ferreira dos, Vivien Thiemy Sakai, Maria Aparecida de Andrade Moreira Machado, Daniela Nicole Schippers, and Andrew Seth Greene. "Reverse transcription and polymerase chain reaction: principles and applications in dentistry." Journal of Applied Oral Science 12, no. 1 (2004): 1–11. http://dx.doi.org/10.1590/s1678-77572004000100002.

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Various molecular biology techniques have become available in the last few years. One of the most revolutionary of these techniques regarding nucleic acid analysis is the polymerase chain reaction (PCR), which was first described in 1985. This method relies on the exponential amplification of specific DNA fragments, resulting in millions of copies that can serve as templates for different kinds of analyses. PCR can be preceded by a reverse transcription (RT) reaction in order to produce cDNA from RNA (RT-PCR). RT-PCR provides the possibility to assess gene transcription in cells or tissues. PC

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Cross, N. C. P., Feng Lin, and J. M. Goldman. "APPROPRIATE CONTROLS FOR REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION (RT-PCR)." British Journal of Haematology 87, no. 1 (1994): 218. http://dx.doi.org/10.1111/j.1365-2141.1994.tb04899.x.

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Yang, Shuixian, Kai-Yu Wang, and Zexiao Yang. "Comparison of reverse-transcription loop-mediated isothermal amplification and reverse-transcription polymerase chain reaction for grass carp reovirus." Acta Veterinaria Brno 84, no. 3 (2015): 215–23. http://dx.doi.org/10.2754/avb201584030215.

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Grass carp reovirus (GCRV) has been assigned to a newly established Aquareovirus genus in the family of Reoviridae which leads to haemorrhagic disease and extremely high mortality rate in grass carp. In this study, comparison was made between the novel one-step reverse transcription loop-mediated isothermal amplification (RT-LAMP) and the reverse transcription polymerase chain reaction (RT-PCR) for detection of grass carp reovirus. The result indicated that RT-LAMP had × 10 higher sensitivity comparable to RT-PCR. The specificity of the two methods for GCRV detection were both developed succes

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Paramata, Nanang Roswita, Winansih Gubali, and Rahma Yulia Adipu. "Results of Reverse Transcription-Polymerase Chain Reaction (RT-PCR) Examination in Covid-19 Patients at Prof. Dr. H. Aloei Saboe City of Gorontalo." Jambura Nursing Journal 5, no. 1 (2023): 86–93. http://dx.doi.org/10.37311/jnj.v5i1.18323.

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Coronavirus Disease or COVID-19 is still a concern throughout the world. WHO recommends the Reverse Transcription-Polymerase Chain Reaction (RT-PCR) method as the gold standard for diagnosing COVID-19. The purpose of this study was to describe the results of the Reverse Transcription-Polymerase Chain Reaction (RT-PCR) examination in COVID-19 patients at Prof. Dr. H. Aloe Saboe, City of Gorontalo. This research is a type of descriptive qualitative research with a retrospective approach. The population of this study were all suspected and confirmed COVID-19 patients treated at Prof. Hospital. Dr

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Wang, Gehua, Connie Barton, and Frank G. Rodgers. "Bacterial DNA decontamination for reverse transcription polymerase chain reaction (RT-PCR)." Journal of Microbiological Methods 51, no. 1 (2002): 119–21. http://dx.doi.org/10.1016/s0167-7012(02)00041-6.

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Bustin, SA. "Absolute quantification of mRNA using real-time reverse transcription polymerase chain reaction assays." Journal of Molecular Endocrinology 25, no. 2 (2000): 169–93. http://dx.doi.org/10.1677/jme.0.0250169.

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The reverse transcription polymerase chain reaction (RT-PCR) is the most sensitive method for the detection of low-abundance mRNA, often obtained from limited tissue samples. However, it is a complex technique, there are substantial problems associated with its true sensitivity, reproducibility and specificity and, as a quantitative method, it suffers from the problems inherent in PCR. The recent introduction of fluorescence-based kinetic RT-PCR procedures significantly simplifies the process of producing reproducible quantification of mRNAs and promises to overcome these limitations. Neverthe

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Yoo, Hee-Min, Il-Hwan Kim, and Seil Kim. "Nucleic Acid Testing of SARS-CoV-2." International Journal of Molecular Sciences 22, no. 11 (2021): 6150. http://dx.doi.org/10.3390/ijms22116150.

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The coronavirus disease 2019 (COVID-19) has caused a large global outbreak. It is accordingly important to develop accurate and rapid diagnostic methods. The polymerase chain reaction (PCR)-based method including reverse transcription-polymerase chain reaction (RT-PCR) is the most widely used assay for the detection of SARS-CoV-2 RNA. Along with the RT-PCR method, digital PCR has emerged as a powerful tool to quantify nucleic acid of the virus with high accuracy and sensitivity. Non-PCR based techniques such as reverse transcription loop-mediated isothermal amplification (RT-LAMP) and reverse

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Al-Shamary, Abd Al-Hakeem A. "SEROLOGICAL AND MOLECULAR DETECTION OF COWPAE MOSAIC VIRUS INFECTING COWPAE IN IRAQ." Diyala Agricultural Sciences Journal 11, no. 2 (2019): 9–17. http://dx.doi.org/10.52951/dasj.19110202.

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This study was conducted to detect CPMV in infecting cowpea plantes at Plant Protection Department/College of Agriculture, University of Baghdad. Symptomatic cowpea plants collected from fields in Baghdad and Diyala Provinces were tested by Emzyme-linked immunosorbent assay (ELISA), reverse transcription- polymerase chain reaction RT-PCR and Immuno-capture reverse transcription-polymerase chain reaction (IC-RT-PCR) using commercial kits. ELISA approach could detect Cowpea mosaic virus CpMV in tested samples but with virus concentration lower than the positive control supplied with the kit, ind

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Jang, Seong Sik, Ji Yeong Noh, Van Thi Lo, et al. "The Epidemiological Characteristics of the Korean Bat Paramyxovirus between 2016 and 2019." Microorganisms 8, no. 6 (2020): 844. http://dx.doi.org/10.3390/microorganisms8060844.

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Bats are considered reservoirs of severe emerging human pathogens. Notably, bats host major mammalian paramyxoviruses from the family Paramyxoviridae, order Mononegavirales. In this study, paramyxoviruses were investigated by reverse transcription semi-nested polymerase chain reaction (RT-semi-nested PCR) and reverse transcription polymerase chain reaction (RT-PCR), based on the RT-semi-nested PCR using the consensus paramyxovirus primers targeting the RNA dependent-RNA-polymerase (RdRp) region. In addition, RT-PCR was performed using newly designed primers targeting regions of the fusion prot

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Haber, S. "Diagnosis of Flame Chlorosis by Reverse Transcription-Polymerase Chain Reaction (RT-PCR)." Plant Disease 79, no. 6 (1995): 626. http://dx.doi.org/10.1094/pd-79-0626.

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Gajardo, R., R. M. Pintó, and A. Bosch. "Polymerase chain reaction amplification and typing of rotavirus in environmental samples." Water Science and Technology 31, no. 5-6 (1995): 371–74. http://dx.doi.org/10.2166/wst.1995.0643.

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A reverse transcription polymerase chain reaction (RT-PCR) assay is described that has been developed for the detection and serotyping of group A rotavirus in stool specimens and concentrated and non-concentrated sewage specimens.

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Chaudhary, R., S. Bhatta, A. Singh, et al. "A Comparative Study of Rapid SARS-Cov-2 Antigen Detection Assay against RT-PCR Assay for Diagnosis of COVID-19 in a Tertiary Hospital of Kathmandu." Kathmandu University Medical Journal 20, no. 3 (2022): 337–41. http://dx.doi.org/10.3126/kumj.v20i3.53954.

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Background The Coronavirus disease 2019 (COVID-19) pandemic caused by the severe acute respiratory syndrome coronavirus (SARS-CoV-2) has spread worldwide since its first recorded case in the city of Wuhan, China, in December 2019. SARS-CoV-2 infection causes asymptomatic to sever pneumonia. Severe cases may develop acute respiratory disease syndrome (ARDS), with an average mortality rate of 6.9%. Real Time Reverse Transcriptase Polymerase Chain Reaction (rRT-PCR) assay is the current reference standard laboratory method for the diagnosis of SARS-CoV-2 infection. However, it takes around 6-8 ho

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Hadziyannis, Emilia, Nancy Cornish, Colleen Starkey, Gary W. Procop, and Belinda Yen-Lieberman. "Amplicor Enterovirus Polymerase Chain Reaction in Patients With Aseptic Meningitis." Archives of Pathology & Laboratory Medicine 123, no. 10 (1999): 882–84. http://dx.doi.org/10.5858/1999-123-0882-aepcri.

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Abstract Objective.—To evaluate the sensitivity and specificity of reverse-transcription polymerase chain reaction (RT-PCR) and routine cell culture for the detection of enterovirus in cerebrospinal fluid. Methods.—Thirty-eight cerebrospinal fluid specimens were included. Cell culture was inoculated immediately and incubated for 14 days. An aliquot was kept frozen for Amplicor RT-PCR. Chart review was performed to determine the validity of the results. Results.—Nine of 38 specimens were positive for enterovirus by culture, and 14 were positive by RT-PCR. There were 7 discrepancies between the

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Wang, Lih-Chiann, Der-Yuh Lin, Wei Thong, and Ching-Ho Wang. "MULTIPLEX REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION FOR CHICKEN TUMOR VIRUS DETECTION." Taiwan Veterinary Journal 41, no. 04 (2015): 245–49. http://dx.doi.org/10.1142/s168264851550016x.

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Tumor diseases occur frequently in chickens causing a great economic loss. Infected chickens’ pathological lesions are not pathognomonic. This study developed an accurate diagnosis for tumor diseases in chickens. Specific primers to reticuloendotheliosis virus (REV), avian leucosis virus subgroup A (ALV-A), avian leucosis virus subgroup J (ALV-J), and Marek’s disease virus (MDV) were combined into one tube with a single step multiplex reverse transcription polymerase chain reaction (mRT-PCR) performed to amplify the genes from each virus. A total of 117 sample pools containing blood and tissue

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Gebe Abreu Cabral, Paula, Sávio Bastos de Souza, Raul Ferraz Arruda, et al. "Comparative Analysis of Viral Load Quantification Using Reverse Transcription Polymerase Chain Reaction and Digital Droplet Polymerase Chain Reaction." International Journal of Molecular Sciences 26, no. 2 (2025): 725. https://doi.org/10.3390/ijms26020725.

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In the year 2019, a highly virulent coronavirus named severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) emerged, precipitating the outbreak of the illness known as coronavirus disease 2019 (COVID-19). The commonly employed reverse transcription polymerase chain reaction (RT-qPCR) methodology serves to estimate the viral load in each patient’s sample by employing a standard curve. However, it is imperative to recognize that this technique exhibits limitations with respect to clinical diagnosis and therapeutic applications, since an advancement of the conventional polymerase chain rea

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HARA, Masayuki, Nobuyuki HAYASHI, Akari KODAIRA, Shinichiro SHIBATA, and Etsuko UTAGAWA. "Comparison of Norovirus Genotypes by Reverse Transcription-Polymerase Chain Reaction." Kansenshogaku Zasshi 84, no. 6 (2010): 746–48. http://dx.doi.org/10.11150/kansenshogakuzasshi.84.746.

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Abdel-Rady, A. "Molecular diagnosis of peste des petits ruminants virus (PPR) in goats and sheep populations." Journal of the Hellenic Veterinary Medical Society 73, no. 3 (2022): 4627–32. http://dx.doi.org/10.12681/jhvms.28142.

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Peste des petits ruminants (PPR) is an economically important viral disease of goats and sheep. The disease is confused clinically with other infections such as the mild strain of rinderpest in small ruminants. Effective control measures for PPR need that a proper and rapid diagnostic technique of disease. Therefore, the use of reverse transcriptase-polymerase chain reaction (RT-PCR) to detect suspected field samples collected from diseased goats and sheep in Dammam city, Kingdom of Saudia Arabia (KSA) has helped to give an effective diagnosis that was needed to control measure of the spread o

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KUSUNOKI, Mikio, Fumihiro TERAMI, Hidetaka TERAUCHI, and Kazuhiro SOGOU. "Detection of Chrysanthemum Stunt Viroid by the Reverse Transcription-Polymerase Chain Reaction." Annual Report of The Kansai Plant Protection Society 35 (1993): 7–12. http://dx.doi.org/10.4165/kapps1958.35.0_7.

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ROSENFIELD, SORAYA I., and LEE-ANN JAYKUS. "A Multiplex Reverse Transcription Polymerase Chain Reaction Method for the Detection of Foodborne Viruses†." Journal of Food Protection 62, no. 10 (1999): 1210–14. http://dx.doi.org/10.4315/0362-028x-62.10.1210.

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A multiplex reverse transcription polymerase chain reaction (RT-PCR) method was developed for the simultaneous detection of the human enteroviruses, hepatitis A virus (HAV) and Norwalk virus (NV). Poliovirus type 1 (PV1) was chosen as a model for the human enterovirus group. Three different sets of primers were used to produce three size-specific amplicons of 435 bp, 270 bp, and 192 bp for PV1, NV, and HAV, respectively. RT-PCR products were separated by agarose gel electrophoresis, and amplicon identity was confirmed by Southern transfer followed by DNA hybridization using nonradio-active, di

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Zhra, Mahmoud, Aljohara Al Saud, Maha Alzayer, et al. "Cost-effective in-house COVID-19 reverse transcription-polymerase chain reaction testing with yeast-derived Taq polymerase." Annals of Thoracic Medicine 19, no. 2 (2024): 165–71. http://dx.doi.org/10.4103/atm.atm_180_23.

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BACKGROUND: Despite the decline of the COVID-19 pandemic, there continues to be a persistent requirement for reliable testing methods that can be adapted to future outbreaks and areas with limited resources. While the standard approach of using reverse transcription-polymerase chain reaction (RT-PCR) with Taq polymerase is effective, it faces challenges such as limited access to high-quality enzymes and the presence of bacterial DNA contamination in commercial kits, which can impact the accuracy of test results. METHODS: This study investigates the production of recombinant Taq polymerase in y

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Green, Michael R., and Joseph Sambrook. "Quantification of RNA by Real-Time Reverse Transcription-Polymerase Chain Reaction (RT-PCR)." Cold Spring Harbor Protocols 2018, no. 10 (2018): pdb.prot095042. http://dx.doi.org/10.1101/pdb.prot095042.

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YOKOI, Hajime, and Tomoko KITAHASHI. "Astrovirus RNA Detection Using Real-Time Reverse Transcription-Polymerase Chain Reaction." Kansenshogaku Zasshi 83, no. 2 (2009): 120–26. http://dx.doi.org/10.11150/kansenshogakuzasshi.83.120.

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Ishibashi, Toshio, Hiroko Monobe, Masanobu Shinogami, Yuka Nomura, and Jun Yano. "Multiplex Nested Reverse Transcription-Polymerase Chain Reaction for Respiratory Viruses in Acute Otitis Media." Annals of Otology, Rhinology & Laryngology 112, no. 3 (2003): 252–57. http://dx.doi.org/10.1177/000348940311200311.

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Because respiratory viruses play an important role in the causation and pathogenesis of acute otitis media (AOM), determining which virus has infected a child is important with respect to vaccines and antiviral drugs. In some instances, this information might be used to prevent the occurrence of AOM. We used a rapid, economical, and sensitive diagnostic system involving a multiplex nested reverse transcription–polymerase chain reaction (RT-PCR) assay to detect various respiratory viruses in clinical specimens of middle ear fluid (MEF) from children with AOM in our hospital. Multiplex RT-PCR wa

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Choi, Changsun, and Chanhee Chae. "Detection of Classical Swine Fever Virus in Boar Semen by Reverse Transcription–Polymerase Chain Reaction." Journal of Veterinary Diagnostic Investigation 15, no. 1 (2003): 35–41. http://dx.doi.org/10.1177/104063870301500108.

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A seminested reverse transcription–polymerase chain reaction (RT-PCR) was developed for the detection of classical swine fever virus (CSFV) in semen. Five boars were inoculated intranasally with CSFV isolate propagated in PK15 cells. Two boars inoculated with the supernatant of noninfected PK15 cells were kept as controls. Semen and serum samples were collected twice weekly for 63 days postinoculation (dpi). Samples were tested for the presence of antibodies to CSFV by an enzyme-linked immunosorbent assay and for the presence of CSFV nucleic acid by seminested RT-PCR. Antibodies to CSFV could

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Liu, J., K. Qu, C. Chai, H. Li, A. Sferruzza, and R. A. Bender. "Real-time reverse transcription-polymerase chain reaction for detection of SYT-SSX translocation in synovial sarcoma." Journal of Clinical Oncology 24, no. 18_suppl (2006): 9553. http://dx.doi.org/10.1200/jco.2006.24.18_suppl.9553.

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9553 Background: Synovial sarcoma is the most common non-rhabdomyosarcomatous soft tissue sarcoma in children and adolescents. A specific translocation, t(X;18), induces fusion of the SYT gene on chromosome 18 to an SSX gene on chromosome X. The resulting fusion gene consists of at least 2 subtypes with different breakpoints: SYT-SSX1(X;18)(p11.23;q11.2) and SYT-SSX2 (X;18)(p11.21;q11.2). Because t(X;18) transcripts occur in >90% of synovial sarcoma subtypes, this marker may be useful for diagnosis. We evaluated the accuracy of a multiplex real-time reverse transcription-polymerase chain re

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Oksanen, Katri, Heikki Kainulainen, Tarja Ruuska, Markku Mäki, and Merja Ashorn. "Reverse Transcription‐Polymerase Chain Reaction in the Diagnosis of Helicobacter pylori Infection in Finnish Children." Journal of Pediatric Gastroenterology and Nutrition 28, no. 3 (1999): 252–56. http://dx.doi.org/10.1002/j.1536-4801.1999.tb02059.x.

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ABSTRACTBackground:The purpose of this study was to design a simplified polymerase chain reaction (PCR) technique for the detection of Helicobacter pylori and to compare it with conventional diagnostic methods‐culture and histology of gastric biopsy specimens. In addition, the capability of this technique to detect H. pylori in the gastric mucosal biopsies of originally H. pylori‐negative children with gastritis or recurrent abdominal pain was investigated.Methods:Reverse transcriptase polymerase chain reaction (RT‐PCR) using polymerase from Thermus thermophilus was applied to detect H. pylori

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Nie, Xianzhou. "Reverse Transcription Loop-Mediated Isothermal Amplification of DNA for Detection of Potato virus Y." Plant Disease 89, no. 6 (2005): 605–10. http://dx.doi.org/10.1094/pd-89-0605.

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A reverse transcription loop-mediated isothermal amplification of DNA (RT-LAMP) for detection of Potato virus Y (PVY) was developed. In this procedure, a set of four primers matching a total of six sequences of the coat protein (CP) gene of PVY were designed in such a way that a loop could be formed and elongated during DNA amplification. Using PVY CP complementary DNA clones as templates, the LAMP reaction was optimized by adjusting the concentrations of MgSO4, dNTPs, and Bst DNA polymerase. The effects of fragment length of target DNA on LAMP also were investigated. Two-step and one-step RT-

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Wang, Lih-Chiann, Wei-En Hsu, Wei Thong, Ting-Yen Chao, and Ching-Ho Wang. "LOW SPECIFICITY OF A NESTED REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION TO DETECT AVIAN INFLUENZA VIRUS NUCLEOPROTEIN GENE." Taiwan Veterinary Journal 43, no. 02 (2016): 75–79. http://dx.doi.org/10.1142/s168264851550033x.

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Reverse transcription polymerase chain reaction (RT-PCR) was used routinely to detect the avian influenza virus (AIV) nucleoprotein (NP) gene. The purpose of the present study was to compare the correctness of a nested RT-PCR (nRT-PCR), one conventional RT-PCR with its outer primer (oRT-PCR) and the other conventional RT-PCR with its inner primer (iRT-PCR) to detect AIV NP gene. A total of 365 AI-free fecal swabs (73 pools), 7 tracheal swabs and anllantoic fluid from 25 chicken embryos were used to determine the analytic specificities of those tests. Compared with the iRT-PCR, the nRT-PCR was

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Rahman, MM, LR Barman, EH Chowdhury, and MR Islam. "Detection of Newcastle disease virus of poultry by real time reverse transcription-polymerase chain reaction." Bangladesh Veterinarian 33, no. 1 (2017): 16–22. http://dx.doi.org/10.3329/bvet.v33i1.33309.

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A real-time reverse transcription - polymerase chain reaction (rRT-PCR) was used for the detection of Newcastle disease virus (NDV) of poultry. A panel of seven known isolates of NDV in the form of allantoic fluid, obtained from a laboratory repository, was used for the development of the test. RNA was extracted from the allantoic fluid with a magnetic processor based automated RNA extraction system. The identity of the reference virus was first reconfirmed by a conventional RT-PCR specific for the Fusion (F) protein gene. Using these RNA, the rRT-PCR protocol was optimized with regard to the

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Kruse, Abdel-Azim, Kim, et al. "Minimal Residual Disease Detection in Acute Lymphoblastic Leukemia." International Journal of Molecular Sciences 21, no. 3 (2020): 1054. http://dx.doi.org/10.3390/ijms21031054.

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Minimal residual disease (MRD) refers to a chemotherapy/radiotherapy-surviving leukemia cell population that gives rise to relapse of the disease. The detection of MRD is critical for predicting the outcome and for selecting the intensity of further treatment strategies. The development of various new diagnostic platforms, including next-generation sequencing (NGS), has introduced significant advances in the sensitivity of MRD diagnostics. Here, we review current methods to diagnose MRD through phenotypic marker patterns or differential gene patterns through analysis by flow cytometry (FCM), p

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YOSHIO, Hiroyuki, Masao YAMADA, Yoriko YOKOI, Azusa IWAMOTO, Makoto NAKAMURA, and Yumiko MIZOGUCHI. "Diagnosis of Neonatal Enterovirus Meningitis by Reverse Transcription-Polymerase Chain Reaction." Journal of the Japanese Association for Infectious Diseases 71, no. 10 (1997): 1046–50. http://dx.doi.org/10.11150/kansenshogakuzasshi1970.71.1046.

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Xiong, Fei-Fei, Ben-Shang Li, Chun-Xiu Zhang, Hui Xiong, Shu-Hong Shen, and Qing-Hua Zhang. "A Pipeline with Multiplex Reverse Transcription Polymerase Chain Reaction and Microarray for Screening of Chromosomal Translocations in Leukemia." BioMed Research International 2013 (2013): 1–13. http://dx.doi.org/10.1155/2013/135086.

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Chromosome rearrangements and fusion genes present major portion of leukemogenesis and contribute to leukemic subtypes. It is practical and helpful to detect the fusion genes in clinic diagnosis of leukemia. Present application of reverse transcription polymerase chain reaction (RT-PCR) method to detect the fusion gene transcripts is effective, but time- and labor-consuming. To set up a simple and rapid system, we established a method that combined multiplex RT-PCR and microarray. We selected 15 clinically most frequently observed chromosomal rearrangements generating more than 50 fusion gene

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Lekshmi, Manjusha, Sanath H. Kumar, Kooloth Valappil Rajendran, and Binaya Bhusan Nayak. "Development of a reverse transcription (RT) polymerase chain reaction (PCR) method for the detection of human norovirus in bivalve molluscs." Water Science and Technology 83, no. 5 (2021): 1103–7. http://dx.doi.org/10.2166/wst.2021.048.

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Abstract Noroviruses are significant seafood-borne pathogens, commonly associated with the consumption of filter feeding bivalve molluscs. Here, we report the development of a reverse transcription polymerase chain reaction (RT-PCR) method using primers based on the RNA-dependent RNA polymerase gene of norovirus genogroup II (NoV GII). Samples of bivalves were processed for the concentration of virus and extraction of RNA, followed by reverse transcription PCR. A total of 50 molluscan shellfish samples were analyzed, of which 16 samples yielded positive amplifications of norovirus nucleic acid

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Bustin, S. A., V. Benes, T. Nolan, and M. W. Pfaffl. "Quantitative real-time RT-PCR – a perspective." Journal of Molecular Endocrinology 34, no. 3 (2005): 597–601. http://dx.doi.org/10.1677/jme.1.01755.

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The real-time reverse transcription polymerase chain reaction (RT-PCR) uses fluorescent reporter molecules to monitor the production of amplification products during each cycle of the PCR reaction. This combines the nucleic acid amplification and detection steps into one homogeneous assay and obviates the need for gel electrophoresis to detect amplification products. Use of appropriate chemistries and data analysis eliminates the need for Southern blotting or DNA sequencing for amplicon identification. Its simplicity, specificity and sensitivity, together with its potential for high throughput

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Almeida, Sérgio Monteiro de, Sônia Mara Raboni, Meri Bordignon Nogueira, and Luine R. Renaud Vidal. "Red blood cells in cerebrospinal fluid as possible inhibitory factor for enterovirus RT-PCR." Arquivos de Neuro-Psiquiatria 74, no. 10 (2016): 810–15. http://dx.doi.org/10.1590/0004-282x20160119.

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ABSTRACT The presence of hemoglobin in samples are considered an important inhibitory factor for polymerase chain reaction (PCR). The aim of this study was to examine the influence of red blood cells (RBC)s in cerebrospinal fluid (CSF) as an inhibitory factor to reverse transcription polymerase chain reaction (RT-PCR) for enteroviruses (EV). Forty-four CSF samples from patients showing characteristics of viral meningitis were assessed for EV by RT-PCR. Viral RNA extracted with guanidine isothyocianate buffer and virus detection was performed by in-house nested PCR. Positivity for EV RT-PCR was

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Chang, H. L., M. H. Zaroukian, and W. J. Esselman. "T200 alternate exon use in murine lymphoid cells determined by reverse transcription-polymerase chain reaction." Journal of Immunology 143, no. 1 (1989): 315–21. http://dx.doi.org/10.4049/jimmunol.143.1.315.

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Abstract T200 glycoproteins of lymphoid and myeloid cells exhibit cell lineage-specific structural heterogeneity. Peptide heterogeneity appears to arise from alternate 5'-exon use (Ex-4, 5, and 6), potentially giving rise to eight distinct forms of T200 mRNA containing 0 to 3 of these alternate exons. A method is described for determining the number and identity of the three alternate T200 exons expressed in cells by using the polymerase chain reaction (PCR) and the reverse transcription-polymerase chain reaction (RT-PCR) without prior purification of RNA. Synthetic primers flanking the altern

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Green, Michael R., and Joseph Sambrook. "Amplification of cDNA Generated by Reverse Transcription of mRNA: Two-Step Reverse Transcription-Polymerase Chain Reaction (RT-PCR)." Cold Spring Harbor Protocols 2019, no. 5 (2019): pdb.prot095190. http://dx.doi.org/10.1101/pdb.prot095190.

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Akimkin, V. G., V. V. Petrov, K. V. Krasovitov, et al. "Molecular methods for diagnosing novel coronavirus infection: comparison of loop-mediated isothermal amplification and polymerase chain reaction." Problems of Virology 66, no. 6 (2022): 417–24. http://dx.doi.org/10.36233/0507-4088-86.

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Introduction. Currently, the basis for molecular diagnostics of most infections is the use of reverse transcription polymerase chain reaction (RT-PCR). Technologies based on reverse transcription isothermal loop amplification (RT-LAMP) can be used as an alternative to RT-PCR for diagnostic purposes. In this study, we compared the RTLAMP and RT-PCR methods in order to analyze both the advantages and disadvantages of the two approaches.Material and methods. For the study, we used reagent kits based on RT-PCR and RT-LAMP. The biological material obtained by taking swabs from the mucous membrane o

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Sudweeks, Sterling N., and Roy E. Twyman. "Single cell reverse transcription-polymerase chain reaction (RT-PCR) and the GABA-A receptor." Neurochemistry International 28, no. 2 (1996): 137–39. http://dx.doi.org/10.1016/0197-0186(95)00076-3.

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Mills, Margaret G., Emily Bruce, Meei-Li Huang, et al. "An international, interlaboratory ring trial confirms the feasibility of an extraction-less “direct” RT-qPCR method for reliable detection of SARS-CoV-2 RNA in clinical samples." PLOS ONE 17, no. 1 (2022): e0261853. http://dx.doi.org/10.1371/journal.pone.0261853.

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Reverse transcription–quantitative polymerase chain reaction (RT-qPCR) is used worldwide to test and trace the spread of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). “Extraction-less” or “direct” real time–reverse transcription polymerase chain reaction (RT-PCR) is a transparent and accessible qualitative method for SARS-CoV-2 detection from nasopharyngeal or oral pharyngeal samples with the potential to generate actionable data more quickly, at a lower cost, and with fewer experimental resources than full RT-qPCR. This study engaged 10 global testing sites, including laborato

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Margaria, P., C. Rosa, C. Marzachì, M. Turina, and S. Palmano. "Detection of Flavescence Dorée Phytoplasma in Grapevine by Reverse-Transcription PCR." Plant Disease 91, no. 11 (2007): 1496–501. http://dx.doi.org/10.1094/pdis-91-11-1496.

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Flavescence dorée (FD) is the most serious phytoplasma disease of grapevine. This report describes a novel method of detecting FD phytoplasma based on reverse-transcription polymerase chain reaction (RT-PCR) on 16S ribosomal RNA (16SrRNA) which will greatly improve mass screening of infected grapevines. A rapid protocol for extracting sap from whole leaves or midveins and successive one-tube amplification by RT-PCR was applied to grapevine samples with or without symptoms collected from different areas of Piedmont (northwestern Italy). Results were compared with those obtained using one of the

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Turgut, Burak. "Role of ophthalmologists in combating with the Coronavirus disease 2019." Advances in Ophthalmology & Visual System 10, no. 2 (2020): 31–34. http://dx.doi.org/10.15406/aovs.2020.10.00378.

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nCoV, novel coronavirus; SARS-CoV, severe acute respiratory syndrome Coronavirus; MERS-CoV, Middle East Respiratory Syndrome-Coronavirus; 2019-nCoV, novel Coronavirus 2019; WHO, World Health Organization; ACE2, angiotensin‐converting enzyme 2; reverse‐transcription polymerase chain reaction (RT‐PCR); CDC, Centers for Disease Control and Prevention; AAO, American Academy of Ophthalmology.

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Jian, Ming-Jr, Chi-Sheng Chen, Hsing-Yi Chung, Chih-Kai Chang, Cherng-Lih Perng, and Hung-Sheng Shang. "Clinical Evaluation of Direct Reverse Transcription PCR for Detection of SARS-CoV-2 Compared to Conventional RT-PCR in Patients with Positive Rapid Antigen Test Results during Circulation of Emerging Viral Variants." Diagnostics 13, no. 24 (2023): 3668. http://dx.doi.org/10.3390/diagnostics13243668.

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The emergence of the Omicron (B.1.1.529) variant of SARS-CoV-2 has precipitated a new global wave of the COVID-19 pandemic. The rapid identification of SARS-CoV-2 infection is imperative for the effective mitigation of transmission. Diagnostic modalities such as rapid antigen testing and real-time reverse transcription polymerase chain reaction (RT-PCR) offer expedient turnaround times of 10–15 min and straightforward implementation. This preliminary study assessed the correlation between outcomes of commercially available rapid antigen tests for home use and conventional reverse transcription

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Forma, Ewa, Magdalena Bernaciak, Hanna Romanowicz-Makowska, and Magdalena Bryś. "RT-PCR Analysis of TopBP1 Gene Expression in Hereditary Breast Cancer." Folia Biologica et Oecologica 6 (December 4, 2010): 49–59. http://dx.doi.org/10.2478/v10107-009-0006-1.

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Hereditary predisposition to breast cancer determined in large part by loss of function mutations in one of two genes BRCA1 and BRCA2. Besides BRCA1 and BRCA2 other genes are also likely to be involved in hereditary predisposition to breast cancer. TopBP1 protein is involved in DNA replication, DNA damage checkpoint response and transcriptional regulation. Expression of TopBP1 gene at the mRNA level was analyzed by semiquantitative reverse transcription-polymerase chain reaction (RT-PCR) in 94 samples of hereditary breast cancer. Analysis of TopBP1 mRNA level showed that expression of TopBP1 i

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Janjua, Sana Iqbal, Muhammad Younas, Raja Kamran Afzal, Asma Khan, Farhat Yasmeen, and Muskan Younas. "Frequency of SARS-CoV-2 Antibodies in PCR-Positive COVID-19 Patients and High-Risk Exposed Subjects at Multan." Pakistan Armed Forces Medical Journal 73, no. 4 (2023): 1095–99. http://dx.doi.org/10.51253/pafmj.v73i4.8820.

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  Objective: To investigate the degree of association between Reverse Transcription Polymerase Chain Reaction positivity and seroconversion after natural COVID-19 infection in Multan, City of Pakistan. Study Design: Cross-sectional study. Place and Duration of Study: Combined Military Hospital, Multan Pakistan from Apr 2021 to Sep 2021. Methodology: In this study, 219 Healthcare Workers with suspected SARS-CoV-2 infection were screened via Reverse Transcription Polymerase Chain Reaction for viral genome, followed by detection of corresponding antibody response in

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Chen, Jyh-Jian, and Zong-Hong Lin. "Fabrication of an Oscillating Thermocycler to Analyze the Canine Distemper Virus by Utilizing Reverse Transcription Polymerase Chain Reaction." Micromachines 13, no. 4 (2022): 600. http://dx.doi.org/10.3390/mi13040600.

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The reverse transcription-polymerase chain reaction (RT-PCR) has been utilized as an effective tool to diagnose the infectious diseases of viruses. In the present work, the oscillating thermocycler is fabricated and performed to carry out the one-step RT-PCR process successfully. The ribonucleic acid (RNA) mixture is pipetted into the fixed sample volume inside an aluminum reaction block. The sample oscillates the pathway onto the linear motion control system and through the specific RT-PCR heating zones with individual homemade thermal control modules. The present oscillating thermocycler com

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JANULIS, LYNN, JANICE M. BAHR, REX A. HESS, and DAVID BUNICK. "P450 Aromatase Messenger Ribonucleic Acid Expression in Male Rat Germ Cells: Detection by Reverse Transcription‐Polymerase Chain Reaction Amplification." Journal of Andrology 17, no. 6 (1996): 651–58. http://dx.doi.org/10.1002/j.1939-4640.1996.tb01849.x.

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ABSTRACT: We have previously demonstrated that cytochrome P450 aromatase (P450arom) protein, an estrogen‐synthesizing enzyme, is present and active in germ cells of the adult mouse testis. To establish that P450arom mRNA is expressed in germ cells of other species, we examined expression of P450arom in adult rat germ cells by employing reverse transcription‐polymerase chain reaction (RT‐PCR). Total RNA was extracted from Sta‐put separated germ cells and reverse transcribed. The resulting cDNA was amplified by nested PCR reactions using oligonucleotide primers selected from a highly conserved r

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Nakura, Yukiko, Heng Ning Wu, Yuya Okamoto, et al. "Development of an efficient one-step real-time reverse transcription polymerase chain reaction method for severe acute respiratory syndrome-coronavirus-2 detection." PLOS ONE 16, no. 6 (2021): e0252789. http://dx.doi.org/10.1371/journal.pone.0252789.

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The general methods to detect the RNA of severe acute respiratory syndrome-coronavirus 2 (SARS-CoV-2) in clinical diagnostic testing involve reverse transcriptases and thermostable DNA polymerases. In this study, we compared the detection of SARS-CoV-2 by a one-step real-time RT-PCR method using a heat-resistant reverse transcriptase variant MM4 from Moloney murine leukemia virus, two thermostable DNA polymerase variants with reverse transcriptase activity from Thermotoga petrophila K4 and Thermococcus kodakarensis KOD1, or a wild-type DNA polymerase from Thermus thermophilus M1. The highest p

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