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Articles de revues sur le sujet "Solid fermentation and submerged"

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Colla, Luciane Maria, Aline M. M. Ficanha, Juliana Rizzardi, Telma Elita Bertolin, Christian Oliveira Reinehr et Jorge Alberto Vieira Costa. « Production and Characterization of Lipases by Two New Isolates ofAspergillusthrough Solid-State and Submerged Fermentation ». BioMed Research International 2015 (2015) : 1–9. http://dx.doi.org/10.1155/2015/725959.

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Due to the numerous applications of lipases in industry, there is a need to study their characteristics, because lipases obtained from different sources may present different properties. The aim of this work was to accomplish the partial characterization of lipases obtained through submerged fermentation and solid-state fermentation by two species ofAspergillus. Fungal strains were isolated from a diesel-contaminated soil and selected as good lipases producers. Lipases obtained through submerged fermentation presented optimal activities at 37°C and pH 7.2 and those obtained through solid-state fermentation at 35°C and pH 6.0. The enzymes produced by submerged fermentation were more temperature-stable than those obtained by solid-state fermentation, presenting 72% of residual activity after one hour of exposition at 90°C. Lipases obtained through submerged fermentation had 80% of stability in acidic pH and those obtained through solid-state fermentation had stability greater than 60% in alkaline pH.
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Sehrawat, Rachna, Parmjit S. Panesar, Reeba Panesar et Anit Kumar. « Biopigment produced by Monascus purpureus MTCC 369 in submerged and solid state fermentation : a comparative study ». Pigment & ; Resin Technology 46, no 6 (6 novembre 2017) : 425–32. http://dx.doi.org/10.1108/prt-10-2016-0095.

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Purpose Biopigments, natural colors from microbiological origin are of great interest because of their potential advantages over synthetic colorants. Therefore, this paper aims to evaluate the best possible fermentative conditions for the maximum production of biopigment using solid state fermentation and submerged fermentation by Monascus purpureus MTCC 369. Design/methodology/approach The biopigment was produced using solid state fermentation and submerged with optimized substrate to achieve higher yield. The statistical analysis was carried out using a Microsoft Excel ® (Microsoft Corporation). Findings On comparative analysis, it was observed that solid state fermentation resulted significant accumulation of biopigment (9.0 CVU/g) on the 9th day in comparison to submerged fermentation (5.1 CVU/g) on the 15th day. Practical implications Results revealed that sweet potato peel powder and pea pods provides necessary nutrients required for mycelial growth, and biopigment production, therefore, can be used as potent substrate for biopigment production by Monascus purpureus MTCC 369. Extracted color can be used in confectionery, beverages and pharmaceutical industries. Originality/value This work focuses on utilisation of waste for production of pigment as alternative source to synthetic colorant, and few studies have been carried out using wastes, but no work has been carried out on sweet potato peel to the best of the authors’ knowledge.
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Jovanovic, Mirjana, Damjan Vucurovic, Bojana Bajic, Sinisa Dodic, Vanja Vlajkov et Rada Jevtic-Mucibabic. « Optimization of the simultaneous production of cellulase and xylanase by submerged and solid-state fermentation of wheat chaff ». Journal of the Serbian Chemical Society 85, no 2 (2020) : 177–89. http://dx.doi.org/10.2298/jsc190530080j.

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Wheat chaff as an agricultural waste represents a cheap raw material for biotechnological processes. With its lignocellulosic composition, it is suitable for producing hydrolytic enzymes for second generation renewable fuel production technologies. The aim of this work was to optimize the process parameters (cultivation temperature 25?35?C, pH value 4?6 and cultivation time 3?7 days) of the cultivating fungi (Trichoderma reesei QM 9414) on a media based on wheat chaff by submerged and solid-state techniques, in order to enhance and compare the two types of simultaneous cellulase and xylanase production. Optimal conditions for the submerged fermentation were 29.65?C for temperature, pH 4.27 and 7 days of cultivation, while for the solid-state fermentation, the optimal conditions were 28.01?C, pH 6.00 and 7 days. The cellulolytic and xylanolytic activities of the obtained cultivation broth filtrates were 0.0535 and 0.1676 U mL-1 for the submerged fermentation, and 0.0407 and 0.1401 U mL-1 for the solid-state fermentation, respectively, and with a 26.77 and 13.39 % enhancement of enzyme activity for submerged fermentation, and a 22.96 and 42.66 % enhancement for solid-state fermentation, respectively, compared to the results obtained before optimization.
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Shivanna, Gunashree B., et Govindarajulu Venkateswaran. « Phytase Production byAspergillus nigerCFR 335 andAspergillus ficuumSGA 01 through Submerged and Solid-State Fermentation ». Scientific World Journal 2014 (2014) : 1–6. http://dx.doi.org/10.1155/2014/392615.

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Fermentation is one of the industrially important processes for the development of microbial metabolites that has immense applications in various fields. This has prompted to employ fermentation as a major technique in the production of phytase from microbial source. In this study, a comparison was made between submerged (SmF) and solid-state fermentations (SSF) for the production of phytase fromAspergillus nigerCFR 335 andAspergillus ficuumSGA 01. It was found that both the fungi were capable of producing maximum phytase on 5th day of incubation in both submerged and solid-state fermentation media.Aspergillus nigerCFR 335 andA. ficuumproduced a maximum of 60.6 U/gds and 38 U/gds of the enzyme, respectively, in wheat bran solid substrate medium. Enhancement in the enzyme level (76 and 50.7 U/gds) was found when grown in a combined solid substrate medium comprising wheat bran, rice bran, and groundnut cake in the ratio of 2 : 1 : 1. A maximum of 9.6 and 8.2 U/mL of enzyme activity was observed in SmF byA. nigerCFR 335 andA.ficuum, respectively, when grown in potato dextrose broth.
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Knezevic, Aleksandar, Ivan Milovanovic, Mirjana Stajic et Jelena Vukojevic. « Ligninolytic enzyme production by Lenzites betulinus on selected plant raw materials ». Zbornik Matice srpske za prirodne nauke, no 120 (2011) : 333–38. http://dx.doi.org/10.2298/zmspn1120333k.

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To get a better insight into the ligninolytic system of Lenzites betulinus, the effect of wheat straw and oak sawdust, as carbon sources, on production of Mn-oxidizing peroxidases and laccase, under solid-state and submerged fermentation, was studied. Obtained results revealed considerable differences related to the both factors affecting enzyme activities. Wheat straw was more favorable carbon source for Mn-oxidizing peroxidases and oak sawdust for laccase activity. Solid-state fermentation of wheat straw was optimal for Mn-dependent peroxidase activity (72.1 Ul-1). In contrary to this, submerged fermentation of the same residue gave the highest level of versatile peroxidase activity (25.4 Ul-1). The peak of laccase activity was noted during solid-state fermentation of oak sawdust (32.3 Ul-1), while this enzyme was not detected under submerged fermentation of any plant residues.
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Indrastuti, Erning, Teti Estiasih, Elok Zubaidah et Harijono. « Physicochemical Characteristics and In Vitro Starch Digestibility of Spontaneously Combined Submerged and Solid State Fermented Cassava (Manihot esculenta Crantz) Flour ». Current Nutrition & ; Food Science 15, no 7 (12 novembre 2019) : 725–34. http://dx.doi.org/10.2174/1573401314666180515112908.

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Background: High cyanide varieties of cassava must be detoxified before consumption. Several studies showed detoxification of cassava by slicing, submerged fermentation (soaking), solid state fermentation, and drying. One of traditional detoxification is combination of submerged and solid state fermentation and the effect of this processing on cyanide reduction and food properties has not been evaluation yet. Objective: This research studied the effect of solid state fermentation time on physicochemical, starch granule morphology, and in vitro starch digestibility of cassava flour from high cyanide varieties of Malang 4, Malang 6, and Sembung. Methods: Three varieties of high cyanide grated cassavas were soaked for 3 days in ratio of water to cassava 1:1. After draining for 1 hour, grated cassava was placed in a bamboo container and put in a humid place for 3-day solid state fermentation. Fermented grated cassavas were then dried, milled, and analyzed. Results: Solid state fermentation similarly affected cyanide reduction and characteristics of cassava flour for three high cyanide varieties. The detoxification process reduced cyanide to 89.70-93.42% and produced flour with a total cyanide of 8.25-10.89 mg HCN eq/kg dry matters, which is safe to consume. Fermentation decreased cyanide, starch content, titratable acidity, swelling power, and solubility; meanwhile pH, amylose content, water absorption, oil absorption, and in vitro starch digestibility increased in all three varieties studied. Submerged fermentation reduced the pH thus inhibiting the degradation of linamarin and cyanohydrin into free HCN. pH value was increased by solid state fermentation, from 4.43 to 6.90 that optimum for linamarin and cyanohydrin degradation into free HCN. The submerged and solid-state fermentation indeuce spontaneous microbial growth that affected chemical composition of cassava flour. The changes of structure and morphology of starch granules affected pasting properties, and Increased in vitro starch digestibility due to damaged granules. Conclusion: Solid-state fermentation reduced cyanide content of all three cassava varieties into the safe level for consumption, and aiso changed chemical, physical, and functional characteristics and starch digestibility of cassava flour.
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Dharani, G., et NS Kumaran. « Amylase Production from Solid State Fermentation and Submerged Liquid Fermentation by Aspergillus niger ». Bangladesh Journal of Scientific and Industrial Research 47, no 1 (22 mai 2012) : 99–104. http://dx.doi.org/10.3329/bjsir.v47i1.7310.

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The purpose of this work is to study the optimized cultural conditions for the production of amylase by Aspergillus niger in solid state and submerged liquid fermentation. Four solid substrates banana peel, corn, potato and tapioca with different moisture conditions were taken for solid state fermentation (SSF). Basal medium was used for submerged liquid fermentation (SLF) with different pH (3 to 8), temperature (25, 30, 35 and 40ºC), carbon concentration (1, 2 and 3 g) and nitrogen source (0.1, 0.2 and 0.3 g). In SSF, tapioca yielded highest amylase activity and specific activity (4.43U/ml and 4.58U/mg) at 50% moisture content. In SLF, 2 g starch and 0.3 g peptone concentration showed 0.78 and 1.23 U/ml amylase activities under the optimum pH (5) and temperature (30ºC) the amylase activities reached to 0.86 U/ml and 0.76 U/ml respectively. In SSF using tapioca as substrate the enzyme yield is about five times higher than that achieved with submerged liquid culture. DOI: http://dx.doi.org/10.3329/bjsir.v47i1.7310 Bangladesh J. Sci. Ind. Res. 47(1), 99-104, 2012
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Tarnopol’skaya, Veronika Valentinovna, Tat'yana Vasil'yevna Ryazanova, Natal'ya Yur'yevna Demidenko et Oksana Nikolayevna Eryomenko. « TECHNOLOGY OF PLANT RAW MATERIALS MICROBIOLOGICAL PROCESSING WITH PLEUROTUS STRAINS FOR FEED PRODUCTS PRODUCTION ». chemistry of plant raw material, no 4 (21 décembre 2020) : 405–14. http://dx.doi.org/10.14258/jcprm.2020048445.

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A technology for pilot production of feed products via microbiological conversion of plant raw materials (mixed substrate of pine sawdust and vegetative part of Jerusalem artichoke) by Plerotus ostreatus PO-4.1 and Pleurotus djamor PD-3.2 strains is developed. The technology includes hydrodynamic activation of substrate at the seed stock production stage. The overall technology includes three key stages: submerged fermentation of pure cultures of production strains; submerged-solid phase fermentation of hydrodynamicly activated plant raw materials for seed stock production; solid-state fermentation of mechanically ground plant substrate for feed products production. A successful approbation of submerged-solid state fermentation of production strains on media containing 3% of hydrodynamicly activated raw materials allowed for obtaining seed stock with 14.5 g/l yield of submerged mycelium biomass fully adopted for this type of substrate. Further use of this seed stock biomass at the solid state fermentation stage makes the overall process duration three times shorter compared to existing technologies for direct wood waste bioconversion. The pilot plant results show valuable practicability of plant raw material hydrodynamic activation with the purpose of enhancing its bioaccessibility with consequent increase in degree of microbiological conversion. The product of bioconversion contains 14–16% of protein, biofiber, vitamins and minerals and could be considered for successful use as feed by agricultural enterprises.
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Martău, Gheorghe-Adrian, Peter Unger, Roland Schneider, Joachim Venus, Dan Cristian Vodnar et José Pablo López-Gómez. « Integration of Solid State and Submerged Fermentations for the Valorization of Organic Municipal Solid Waste ». Journal of Fungi 7, no 9 (16 septembre 2021) : 766. http://dx.doi.org/10.3390/jof7090766.

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Solid state fermentation (SsF) is recognized as a suitable process for the production of enzymes using organic residues as substrates. However, only a few studies have integrated an evaluation of the feasibility of applying enzymes produced by SsF into subsequent hydrolyses followed by the production of target compounds, e.g., lactic acid (LA), through submerged-liquid fermentations (SmF). In this study, wheat bran (WB) was used as the substrate for the production of enzymes via SsF by Aspergillus awamori DSM No. 63272. Following optimization, cellulase and glucoamylase activities were 73.63 ± 5.47 FPU/gds and 107.10 ± 2.63 U/gdb after 7 days and 5 days of fermentation, respectively. Enzymes were then used for the hydrolysis of the organic fraction of municipal solid waste (OFMSW). During hydrolysis, glucose increased considerably with a final value of 19.77 ± 1.56 g/L. Subsequently, hydrolysates were fermented in SmF by Bacillus coagulans A166 increasing the LA concentration by 15.59 g/L. The data reported in this study provides an example of how SsF and SmF technologies can be combined for the valorization of WB and OFMSW.
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López-Gómez, José Pablo, et Joachim Venus. « Potential Role of Sequential Solid-State and Submerged-Liquid Fermentations in a Circular Bioeconomy ». Fermentation 7, no 2 (11 mai 2021) : 76. http://dx.doi.org/10.3390/fermentation7020076.

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An efficient processing of organic solid residues will be pivotal in the development of the circular bioeconomy. Due to their composition, such residues comprise a great biochemical conversion potential through fermentations. Generally, the carbohydrates and proteins present in the organic wastes cannot be directly metabolized by microorganisms. Thus, before fermentation, enzymes are used in a hydrolysis step to release digestible sugars and nitrogen. Although enzymes can be efficiently produced from organic solid residues in solid-state fermentations (SsF), challenges in the development and scale-up of SsF technologies, especially bioreactors, have hindered a wider application of such systems. Therefore, most of the commercial enzymes are produced in submerged-liquid fermentations (SmF) from expensive simple sugars. Instead of independently evaluating SsF and SmF, the review covers the option of combining them in a sequential process in which, enzymes are firstly produced in SsF and then used for hydrolysis, yielding a suitable medium for SmF. The article reviews experimental work that has demonstrated the feasibility of the process and underlines the benefits that such combination has. Finally, a discussion is included which highlights that, unlike typically perceived, SsF should not be considered a counterpart of SmF but, in contrast, the main advantages of each type of fermentation are accentuated in a synergistic sequential SsF-SmF.
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Thèses sur le sujet "Solid fermentation and submerged"

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Kennedy, Max James. « The monitoring and evaluation of a solid substrate submerged culture fermentation ». Thesis, Massachusetts Institute of Technology, 1990. http://hdl.handle.net/1721.1/54965.

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Zhuang, Jun. « ECONOMIC ANALYSIS OF CELLULASE PRODUCTION BY CLOSTRIDIUM THERMOCELLUM IN SOLID STATE AND SUBMERGED FERMENTATION ». UKnowledge, 2004. http://uknowledge.uky.edu/gradschool_theses/170.

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Dependence on foreign oil remains a serious issue for the U.S. economy. Additionally, automobile emissions related to petroleum-based, fossil fuel has been cited as one source of environmental problems, such as global warming and reduced air quality. Using agricultural and forest biomass as a source for the biofuel ethanol industry, provides a partial solution by displacing some fossil fuels. However, the use of high cost enzymes as an input is a significant limitation for ethanol production.Economic analyses of cellulase enzyme production costs using solid state cultivation (SSC) are performed and compared to the traditional submerged fermentation (SmF) method. Results from this study indicate that the unit costs for the cellulase enzyme production are $15.67 per kilogram ($/kg) and $40.36/kg, for the SSC and SmF methods, respectively, while the market price for the cellulase enzyme is $36.00/kg. Profitability analysis and sensitivity analysis also provide positive results.Since these results indicate that the SSC method is economical, ethanol production costs may be reduced, with the potential to make ethanol a viable supplemental fuel source in light of current political, economic and environmental issues.
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Martins, Eduardo da Silva. « Purificação e caracterização bioquímica de poligalacturonases termoestáveis produzidas pelo fungo Thermoascus aurantiacus através de fermentação submersa e fermentação em estado sólido / ». Rio Claro : [s.n.], 2006. http://hdl.handle.net/11449/103964.

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Orientador: Eleni Gomes
Banca: Adalbeto Pessoa Júnior
Banca: Maria de Lourdes Teixeira de Moraes Polizeli
Banca: Márcia Regina Brochetto Braga
Banca: Gustavo Orlando Bonilla Rodriguez
Resumo: Pectinases termoestáveis apresentam características interessantes do ponto de vista da sua aplicação industrial, como alta estabilidade ao pH e à temperatura. Além disso, o tipo de processo fermentativo pode influenciar a produção e propriedades físico-químicas destas enzimas. A produção de poligalacturonase (PG) pelo fungo Thermoascus aurantiacus foi realizada em fermentação submersa (FSM) e em estado sólido (FES), usando substratos contendo pectina comercial ou subprodutos agro- industriais como fonte de carbono. A PG bruta obtida em FES apresentou atividade ótima a 65ºC e pH 5,0, com estabilidade na faixa de pH entre 4,0 e 5,0 e entre 7,5 e 8,5 e manteve 85% da atividade original quando incubada a 60ºC, por 1 hora. Em FSM, o melhor meio de cultivo foi a água amarela, com pH inicial de 5,5, após 5 dias de cultivo a 45ºC. A enzima em sua forma bruta apresentou temperatura ótima de 60ºC e pH ótimo de 5,0, maior estabilidade em pH ácido (3,0 a 4,5) e menor termoestabilidade, quando comparada com a obtida em FES, mantendo apenas 13% da atividade original quando incubada a 60ºC, por 1 hora. As enzimas foram purificadas utilizando-se cromatografias de filtração em gel e troca iônica. A PG purificada proveniente da FSM apresentou pH e temperatura ótimos de 5,5 e 60-65ºC, estabilidade em pH 5,0-5,5 e manteve, após 1 hora de incubação, 100% da atividade original até 50ºC. Resultados similares foram obtidos para a PG proveniente da FES. A PG de FES apresentou massa molar de 29,3 kDa, Km de 1,58 mg/mL e Vmáx de 1553,1 ? mol/min/mg, enquanto que a da FSM apresentou massa molar de 30,1 kDa, km de 1,46 mg/mL e Vmáx de 2433,3 ? mol/min/mg. Íons como Fe+3, Ca+2, e K+ praticamente não afetaram a atividade da enzima... (Resumo completo, clicar acesso eletrônico abaixo)
Abstract: Thermostable pectinases present important characteristics under the view of their industrial application, as their high stability to pH and temperature. Besides, the type of fermentative process used can affect the ir production and physical-chemical properties. The polygalacturonase (PG) production by the thermophilic fungus Thermoascus aurantiacus was carried out by submerged fermentation (SMF) and solid state fermentation (SSF) using substrates containing commercial pectin or agro- industrial residues as carbon sources. The crude PG from SSF presented optimum activity at 65ºC and pH 5.0, with stability at pH 4.0-5.0 and 7.5-8.5 and maintained 85% of its original activity at 60º C for 1 hour. In SMF the best cultivation medium was the liquid waste from juice extraction, with initial pH of 5.5, after 5 days of cultivation at 45ºC. The crude enzyme showed an optimum activity at 60ºC and pH 5.0, higher stability in acid ic pH (3.0 to 4.5) and was less thermostable when compared to that obtained in SSF, wich maintained only 13% of its original activity at 60ºC, for 1 hour. Purification of enzymes was carried out using filtration and ion-exchange chromatographies. The purified PG, from SMF, showed optimum pH and temperature of 5.5 and 60-65ºC, stability at pH 5.0-5.5 and preserved, after 1 hour incubation, 100% of its original activity at 50ºC. Similar results were obtained to PG from SSF. The PG obtained by SSF presented molar mass of 29.3 kDa, Km of 1.58 mg/ml and Vmáx of 1553.1 ? mol/min/mg, while that the enzyme from SMF presented molar mass of 30.1 kDa, km of 1.46 mg/ml and Vmáx of 2433.3 ? mol/min/mg. Ions such as Fe3+, Ca2+ and K+ practically did not affect the enzyme activity, while Mg2+, Mn2+ and Zn2+ decreased 7%, 75% and 50%... (Complete abstract, click electronic address below)
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Prevot, Vincent. « Comparaison de la production de complexes enzymatiques par fermentation en milieu solide et par fermentation en milieu liquide ». Thesis, Reims, 2013. http://www.theses.fr/2013REIMS009/document.

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La fermentation en milieu solide est un bioprocédé pouvant éventuellement être utilisé comme technologie de rupture pour diminuer le coût des biocatalyseurs utilisés dans la conversion de biomasse lignocellulosique comme le son de blé. La première partie de ces travaux de recherche a donc étudié le potentiel de cette technologie par rapport à celle de fermentation en milieu submergé lors d'une comparaison en application. Plusieurs tests de saccharification ont ainsi été réalisés sur différentes matières premières (cellulose, son de blé) et ont permis de montrer l'avantage différentiateur des biocatalyseurs produits par fermentation en milieu solide. Ensuite, la deuxième partie de ces travaux de recherche a porté sur l'étude des facteurs de la récalcitrance du son de blé à l'hydrolyse enzymatique. Deux principaux facteurs ont ainsi pu être démontrés : un facteur physique, lié à l'accessibilité des biocatalyseurs aux polysaccharides, et un facteur biochimique, lié à l'absence ou à la faible présence de certaines activités enzymatiques (féruloyl estérase,…) dans le complexe enzymatique de Trichoderma reesei Rut-C30. Cette étude a également permis d'identifier l'origine des différentes fractions glucidiques hydrolysées et de déterminer le potentiel glucidique actuellement hydrolysable à partir de cette biomasse. Enfin, la dernière partie de ces travaux de recherche a été consacrée à l'étude pratique d'un concept innovant de procédé permettant de favoriser la conversion des polysaccharides contenus dans le son de blé. Une levée de la barrière physique au transfert de masse et par conséquent une validation de ce concept a finalement pu être réalisée
Solid-state fermentation is a bioprocess that can optionally be used as disruptive technology to reduce the cost of biocatalysts used in the lignocellulosic biomass conversion like wheat bran. The first part of this research has explored the potential of this technology compared to submerged fermentation in an application comparison. Several saccharification tests have thus been carried on different feedstocks (cellulose, wheat bran) and have shown the differentiator advantage of biocatalysts produced by solid state fermentation. Then, the second part of this research has investigated the recalcitrance factors of wheat bran to enzymatic hydrolysis. Two main factors have thus been demonstrated: a physical factor, related to the accessibility of biocatalysts to the polysaccharides, and a biochemical factor, related to the absence or the low presence of some enzymatic activities (feruloyl esterase, ...) in the enzymatic complex of Trichoderma reesei Rut-C30. This study has also identified the origin of the various carbohydrate moieties hydrolyzed and has determined the carbohydrate potential currently releasable from this biomass. Finally, the last part of this research has been devoted to the practical study of an innovative concept of process to promote the conversion of polysaccharides in wheat bran. A removal of the physical barrier to mass transfer and therefore a validation of this concept has finally been achieved
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Martins, Eduardo da Silva [UNESP]. « Purificação e caracterização bioquímica de poligalacturonases termoestáveis produzidas pelo fungo Thermoascus aurantiacus através de fermentação submersa e fermentação em estado sólido ». Universidade Estadual Paulista (UNESP), 2006. http://hdl.handle.net/11449/103964.

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Made available in DSpace on 2014-06-11T19:32:55Z (GMT). No. of bitstreams: 0 Previous issue date: 2006-06-05Bitstream added on 2014-06-13T19:22:45Z : No. of bitstreams: 1 martins_es_dr_rcla.pdf: 4883436 bytes, checksum: 90e9eddebe5cd615b809d1494e86d994 (MD5)
Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Pectinases termoestáveis apresentam características interessantes do ponto de vista da sua aplicação industrial, como alta estabilidade ao pH e à temperatura. Além disso, o tipo de processo fermentativo pode influenciar a produção e propriedades físico-químicas destas enzimas. A produção de poligalacturonase (PG) pelo fungo Thermoascus aurantiacus foi realizada em fermentação submersa (FSM) e em estado sólido (FES), usando substratos contendo pectina comercial ou subprodutos agro- industriais como fonte de carbono. A PG bruta obtida em FES apresentou atividade ótima a 65ºC e pH 5,0, com estabilidade na faixa de pH entre 4,0 e 5,0 e entre 7,5 e 8,5 e manteve 85% da atividade original quando incubada a 60ºC, por 1 hora. Em FSM, o melhor meio de cultivo foi a água amarela, com pH inicial de 5,5, após 5 dias de cultivo a 45ºC. A enzima em sua forma bruta apresentou temperatura ótima de 60ºC e pH ótimo de 5,0, maior estabilidade em pH ácido (3,0 a 4,5) e menor termoestabilidade, quando comparada com a obtida em FES, mantendo apenas 13% da atividade original quando incubada a 60ºC, por 1 hora. As enzimas foram purificadas utilizando-se cromatografias de filtração em gel e troca iônica. A PG purificada proveniente da FSM apresentou pH e temperatura ótimos de 5,5 e 60-65ºC, estabilidade em pH 5,0-5,5 e manteve, após 1 hora de incubação, 100% da atividade original até 50ºC. Resultados similares foram obtidos para a PG proveniente da FES. A PG de FES apresentou massa molar de 29,3 kDa, Km de 1,58 mg/mL e Vmáx de 1553,1 ? mol/min/mg, enquanto que a da FSM apresentou massa molar de 30,1 kDa, km de 1,46 mg/mL e Vmáx de 2433,3 ? mol/min/mg. Íons como Fe+3, Ca+2, e K+ praticamente não afetaram a atividade da enzima...
Thermostable pectinases present important characteristics under the view of their industrial application, as their high stability to pH and temperature. Besides, the type of fermentative process used can affect the ir production and physical-chemical properties. The polygalacturonase (PG) production by the thermophilic fungus Thermoascus aurantiacus was carried out by submerged fermentation (SMF) and solid state fermentation (SSF) using substrates containing commercial pectin or agro- industrial residues as carbon sources. The crude PG from SSF presented optimum activity at 65ºC and pH 5.0, with stability at pH 4.0-5.0 and 7.5-8.5 and maintained 85% of its original activity at 60º C for 1 hour. In SMF the best cultivation medium was the liquid waste from juice extraction, with initial pH of 5.5, after 5 days of cultivation at 45ºC. The crude enzyme showed an optimum activity at 60ºC and pH 5.0, higher stability in acid ic pH (3.0 to 4.5) and was less thermostable when compared to that obtained in SSF, wich maintained only 13% of its original activity at 60ºC, for 1 hour. Purification of enzymes was carried out using filtration and ion-exchange chromatographies. The purified PG, from SMF, showed optimum pH and temperature of 5.5 and 60-65ºC, stability at pH 5.0-5.5 and preserved, after 1 hour incubation, 100% of its original activity at 50ºC. Similar results were obtained to PG from SSF. The PG obtained by SSF presented molar mass of 29.3 kDa, Km of 1.58 mg/ml and Vmáx of 1553.1 ? mol/min/mg, while that the enzyme from SMF presented molar mass of 30.1 kDa, km of 1.46 mg/ml and Vmáx of 2433.3 ? mol/min/mg. Ions such as Fe3+, Ca2+ and K+ practically did not affect the enzyme activity, while Mg2+, Mn2+ and Zn2+ decreased 7%, 75% and 50%... (Complete abstract, click electronic address below)
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Ferrarezi, Ana Lúcia. « Seleção de fungos termofílicos para produção de lipase e aplicação na produção de biodiesel / ». Rio Claro : [s.n.], 2011. http://hdl.handle.net/11449/103950.

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Orientador: Eleni Gomes
Coorientador: Gustavo O. Bonilla-Rodriguez
Banca: Heizir Ferreira de Castro
Banca: Ernandes Benedito Pereira
Banca: Humberto Márcio Santos Milagre
Banca: Teresa Cristina Zangirolami
Resumo: As enzimas são catalisadores muito eficientes e de grande interesse na aplicação industrial. As lipases (E.C. 3.1.1.3) são serina-hidrolases que agem na hidrólise, esterificação e transesterificação de acilgliceróis de cadeia longa. Lipases microbianas têm sido amplamente usadas devido à sua especificidade. Na transesterificação moléculas de triacilglicerol reagem com um álcool na presença de um catalisador formando uma mistura de glicerol e ésteres de ácidos graxos. O biodiesel, definido como ésteres metílicos ou etílicos, tem atraído crescente interesse como uma fonte de energia renovável, substituindo o diesel produzido a partir de combustíveis fósseis. O presente trabalho tem como objetivo principal efetuar a prospecção de fungos termofílicos que apresentem produção significativa de lipase e, concomitantemente, atividade transesterificante. As linhagens foram selecionadas por detecção de atividade lipolítica em placas de ágar contendo Rodamina B, por fermentação submersa (FSm) e fermentação em estado sólido (FES). Foram testadas linhagens de fungos termofílicos da coleção do laboratório de Bioquímica e Microbiologia Aplicada, sendo Thermomucor indicae-seudaticae N31, Rhizomucor pusillus Myceliophtora sp F2.1.4, Myceliophtora sp M7.7, F2.1.1, F2.1.3 e Thermomyces lanuginosus TO-05 os que mostraram um maior potencial hidrolítico. Estudos adicionais avaliaram a produção de lipase através da modificação da fonte de componentes nutricionais e algumas propriedades físicas na produção de lipase por T. indicae-seudaricae N31 em FSm, e por R. pusillus e T. indicae-seudaticae N31 em FES. Os estudos dos processos fermentativos foram bem sucedidos, havendo um aumento de 16 vezes na produção de lipase de R. pusillus e de 36 vezes na lipase de T indicae-seudaticae... (Resumo completo, clicar acesso eletrônico abaixo)
Abstract: Enzymes are efficient catalysts and interesting for industrial applications. Lipases (EC 3.1.1.3) constitute a group of serine hydrolases that catalyze the hydrolysis, esterification and transesterification reactions of long chain acylglycerols. Microbial lipases have been widely used for biotechnological applications due to their specificity. In transesterification, molecule of a tryacylglicerol react with an alcohol in the presence of catalyst, producing a mixture of fatty esters and glycerol, Biodiesel, defined as methyl or ethyl fatty esters and has attracted considerable attention as a renewable source of energy, in substitution of fossil fuels. The main goal of the present work is the screening of thermophilic fungi that present outstanding lipase production and in parallel able to perform transesterification reaction. Strains were screened for lipase activity on agar plates containing Rhodamine B, for submerged fermentation (SmF) and solid state fermentation (SSF). The tested thermophilic strains were from the collections of the Biochemistry and Applied Microbiology Laboratory, where Thermomucor indicae-seudancae N31, Rhizomucor pusillus Myceliophtora sp F2.1.4, Myceliophtora sp M7.7, F2.1.1, F2.1.3 and Thermomyces lanuginosus TO-O5 had the highest lipase production. Additional studies attempted to improve lipase production by nutrient source modifications and physicals conditions in FmS by T. indicae-seudaticae N31 and in FSS by T. indicae-seudaticae N31 and R. pusillus. The fermentations studies were successful, with a 16 fold enhancement in lipase yield compared to the initial medim from lipase R. pusillus and 36 fold for the lipase T. indicae-seudaticae N31, both in FES. The lipase from T. indicae-seudaticae N31 cultured in SSF and SmF, exhibited maximum lipolytic activity at 40°C and stability for the pH range from 4 to 8. The enzyme produced by FmS presented maximum activity... (Complete abstract click electronic access below)
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Silva, Ronivaldo Rodrigues da. « Fermentação, purificação e caracterização da protease produzida pelo fungo Aspergillus fumigatus Fresenius / ». São José do Rio Preto : [s.n.], 2011. http://hdl.handle.net/11449/94802.

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Orientador: Hamilton Cabral
Banca: Rodrigo Simões Ribeiro Leite
Banca: Fabiana Fonseca Zanoelo
Resumo: A produção de proteases de origem microbiana depende das condições de cultivo e da diversidade bioquímica de cada espécie. Estudos comparativos entre fermentação em estado sólido (FES) e fermentação submersa (FSm) usando farelo de trigo e meio sintético, respectivamente, foram realizados para a determinação dos parâmetros de produção de proteases pelo fungo Aspergillus fumigatus Fresenius. A melhor produção de protease foi em FES no período de 96 horas utilizando farelo de trigo, temperatura de 30 ºC e 1x106 esporos/5g de substrato com 1.517 U/mL. Em FSm o pico de produção foi em pH 6,0, a 30ºC, 5x105 esporos/mL de meio no período de 72 e 96 horas em meio contendo 0,5 e 0,25% de caseína, respectivamente, ambos com 40 U/mL. Conforme a produtividade dos processos fermentativos, o extrato enzimático da FES foi utilizado para estudos de purificação e caracterização bioquímica. Neste estudo, a protease purificada apresentou atividade ótima em pH 7,5 e 50ºC, sendo inibida por Fenil-metil-sulfonil-fluoreto (PMSF) e mais intensamente por antipaína (1,6 µM). Sobre efeito de íons, foi observado modulação da atividade proteolítica, principalmente com inibição por AlCl3, cuja atividade proteolítica residual foi de 18% após incubação com este íon. Na presença de Ditiotreitol (DTT) e uréia houve diminuição da atividade proteolítica, apresentando atividades residuais de 63% em 200 mM de DTT e 10% com 5 M de uréia. Comparativamente, na concentração de 0,1% de cada surfactante estudado, notou-se redução da atividade proteolítica, sendo 97% em presença de Brometo de cetil-trimetil amônio (CTAB), 79% para 4 - (1,1,3,3 - Tetrametilbutil) fenil- polietileno glicol (Triton X-100), 55% com Polyoxyethylenesorbitan monolaurato (Tween-20) e completa redução da atividade (0%) em... (Resumo completo, clicar acesso eletrônico abaixo)
Abstract: The microbial protease production depends on growing conditions and the biochemical diversity of each species. Comparative studies between solid-state fermentation (SSF) and submerged fermentation (SmF) using wheat bran and synthetic medium, respectively, were performed to determine the optimum parameters for protease production by the fungus Aspergillus fumigatus Fresenius. The best protease production was in SSF within 96 hours using wheat bran, temperature 30°C and 1x106 spores/5g of substrate, with 1,517 U/mL. In SmF peak production was at pH 6.0 at 30°C, 5x105 spores/mL of media within 72 and 96 hours in medium containing 0.5 and 0.25% casein, respectively, with 40 U/mL. According to the productivity of the fermentative processes, enzymatic extract was used from SSF to study purification and biochemical characterization. In this study, purified protease showed optimum activity at pH 7.5 and 50°C, and inhibited by Phenylmethylsulfonyl fluoride (PMSF) and more intensely for antipain (1,6 µM). Concerning to the effect of ions, we observed modulation of the proteolytic activity, especially with inhibition by AlCl3, which residual activity was of 18 % after incubation with this ion. In the presence of Dithiothreitol (DTT) and ureia, we observed progressive decrease in proteolytic activity, presenting residual activities of 63% with 200 mM DTT, and 10% with 5 M ureia. Comparatively, in the concentration of 0.1% of each surfactant studied, there was a reduction in the proteolytic activity in 97% in presence of Cetyl trimethylammonium bromide (CTAB), 79% with 4-(1,1,3,3-Tetramethylbutyl)phenyl-polyethylene glycol (Triton X-100), 55% with Polyoxyethylenesorbitan monolaurate (Tween-20) and a complete inactivation in the presence of Sodium dodecyl sulfate... (Complete abstract click electronic access below)
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Habáníková, Kamila. « Využití odpadů rostlinného původu ». Master's thesis, Vysoké učení technické v Brně. Fakulta chemická, 2010. http://www.nusl.cz/ntk/nusl-216669.

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Production of cellulase and polygalacturonase by Aspergillus niger and Aureobasidium pullulans was studied in submerged (SmF) and solid state fermentation (SSF) systems. Substrates used in fermentation systems were mandarin peels and grape pomace. With Aspergillus niger used on grape pomace as a sole carbon source, cellulase production was detected after 72 hours in SSF and after 24 hours in SmF systems. The activity of cellulase per gram of substrate was higher in a submerged than in a solid state fermentation system. The longer time for higher polygalacturonase production was necessary in submerged fermentation systems and polygalacturonase activity was higher in SmF. The SSF fermentation with mandarin peels as a sole carbon source was similar, the highest detected activity of cellulase was determined after 72 hours. Different production of polygalacturonase was observed on mandarin peels in SmF systems. A comparison of enzyme productivities on grape pomace and mandarin peels showed that polygalacturonase activity per gram of substrate is highest in SmF system with mandarin peels as a sole carbon source. With Aureobasidium pullulans used on grape pomace as a sole carbon source, cellulase production was detected after 48 hours in SmF and SSF fermentation systems. The activity of cellulase per gram of substrate was higher in solid state system than in a submerged fermentation system. Longer time for higher polygalacturonase production was necessary in both fermentation systems. Polygalacturonase activity was higher in SmF. The SSF fermentation with mandarin peels as a sole carbon source was similar, the highest detected activity of cellulase was determined after 48 hours. Different production of polygalacturonase was observed on mandarin peels in SmF systems. A comparison of enzyme productivities on grape pomace and mandarin peels showed that polygalacturonase activity per gram of substrate is highest in SmF system with mandarin peels as a sole carbon source. For both systems and both substrates manganese-dependent peroxidase was detected for the first time. Differences in the enzyme synthesis by Aspergillus niger and Aureobasidium pullulans depend on both the substrates used as well as on the fermentation system.
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Ferrarezi, Ana Lúcia [UNESP]. « Seleção de fungos termofílicos para produção de lipase e aplicação na produção de biodiesel ». Universidade Estadual Paulista (UNESP), 2011. http://hdl.handle.net/11449/103950.

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Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
As enzimas são catalisadores muito eficientes e de grande interesse na aplicação industrial. As lipases (E.C. 3.1.1.3) são serina-hidrolases que agem na hidrólise, esterificação e transesterificação de acilgliceróis de cadeia longa. Lipases microbianas têm sido amplamente usadas devido à sua especificidade. Na transesterificação moléculas de triacilglicerol reagem com um álcool na presença de um catalisador formando uma mistura de glicerol e ésteres de ácidos graxos. O biodiesel, definido como ésteres metílicos ou etílicos, tem atraído crescente interesse como uma fonte de energia renovável, substituindo o diesel produzido a partir de combustíveis fósseis. O presente trabalho tem como objetivo principal efetuar a prospecção de fungos termofílicos que apresentem produção significativa de lipase e, concomitantemente, atividade transesterificante. As linhagens foram selecionadas por detecção de atividade lipolítica em placas de ágar contendo Rodamina B, por fermentação submersa (FSm) e fermentação em estado sólido (FES). Foram testadas linhagens de fungos termofílicos da coleção do laboratório de Bioquímica e Microbiologia Aplicada, sendo Thermomucor indicae-seudaticae N31, Rhizomucor pusillus Myceliophtora sp F2.1.4, Myceliophtora sp M7.7, F2.1.1, F2.1.3 e Thermomyces lanuginosus TO-05 os que mostraram um maior potencial hidrolítico. Estudos adicionais avaliaram a produção de lipase através da modificação da fonte de componentes nutricionais e algumas propriedades físicas na produção de lipase por T. indicae-seudaricae N31 em FSm, e por R. pusillus e T. indicae-seudaticae N31 em FES. Os estudos dos processos fermentativos foram bem sucedidos, havendo um aumento de 16 vezes na produção de lipase de R. pusillus e de 36 vezes na lipase de T indicae-seudaticae...
Enzymes are efficient catalysts and interesting for industrial applications. Lipases (EC 3.1.1.3) constitute a group of serine hydrolases that catalyze the hydrolysis, esterification and transesterification reactions of long chain acylglycerols. Microbial lipases have been widely used for biotechnological applications due to their specificity. In transesterification, molecule of a tryacylglicerol react with an alcohol in the presence of catalyst, producing a mixture of fatty esters and glycerol, Biodiesel, defined as methyl or ethyl fatty esters and has attracted considerable attention as a renewable source of energy, in substitution of fossil fuels. The main goal of the present work is the screening of thermophilic fungi that present outstanding lipase production and in parallel able to perform transesterification reaction. Strains were screened for lipase activity on agar plates containing Rhodamine B, for submerged fermentation (SmF) and solid state fermentation (SSF). The tested thermophilic strains were from the collections of the Biochemistry and Applied Microbiology Laboratory, where Thermomucor indicae-seudancae N31, Rhizomucor pusillus Myceliophtora sp F2.1.4, Myceliophtora sp M7.7, F2.1.1, F2.1.3 and Thermomyces lanuginosus TO-O5 had the highest lipase production. Additional studies attempted to improve lipase production by nutrient source modifications and physicals conditions in FmS by T. indicae-seudaticae N31 and in FSS by T. indicae-seudaticae N31 and R. pusillus. The fermentations studies were successful, with a 16 fold enhancement in lipase yield compared to the initial medim from lipase R. pusillus and 36 fold for the lipase T. indicae-seudaticae N31, both in FES. The lipase from T. indicae-seudaticae N31 cultured in SSF and SmF, exhibited maximum lipolytic activity at 40°C and stability for the pH range from 4 to 8. The enzyme produced by FmS presented maximum activity... (Complete abstract click electronic access below)
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Woods, Jeffrey. « The decrease in phenolic content of canola meal and the production of polyphenoloxidase in submerged and solid state fermentation using the white-rot fungus Trametes versicolor ». Thesis, National Library of Canada = Bibliothèque nationale du Canada, 1999. http://www.collectionscanada.ca/obj/s4/f2/dsk1/tape8/PQDD_0003/MQ46621.pdf.

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Livres sur le sujet "Solid fermentation and submerged"

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Steudler, Susanne, Anett Werner et Jay J. Cheng, dir. Solid State Fermentation. Cham : Springer International Publishing, 2019. http://dx.doi.org/10.1007/978-3-030-23675-5.

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Chen, Hongzhang. Modern Solid State Fermentation. Dordrecht : Springer Netherlands, 2013. http://dx.doi.org/10.1007/978-94-007-6043-1.

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Mitchell, David A., Marin Berovič et Nadia Krieger, dir. Solid-State Fermentation Bioreactors. Berlin, Heidelberg : Springer Berlin Heidelberg, 2006. http://dx.doi.org/10.1007/3-540-31286-2.

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Roussos, Sevastianos, B. K. Lonsane, Maurice Raimbault et Gustavo Viniegra-Gonzalez, dir. Advances in Solid State Fermentation. Dordrecht : Springer Netherlands, 1997. http://dx.doi.org/10.1007/978-94-017-0661-2.

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Pandey, Ashok, Carlos Ricardo Soccol et Christian Larroche, dir. Current Developments in Solid-state Fermentation. New York, NY : Springer New York, 2008. http://dx.doi.org/10.1007/978-0-387-75213-6.

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Nigam, Poonam Singh. Microbial biotechnology exploiting solid state fermentation. New York : Kluwer Academic/Plenum Publishers, 2003.

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International Symposium on Solid State Fermentation (2nd 1995 Montpellier, France). Advances in solid state fermentation : Proceedings of the 2nd International Symposium on Solid State Fermentation, FMS-95, Montpellier, France. Dordrecht : Kluwer Academic Publishers, 1997.

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International Symposium on Solid State Fermentation (2nd 1995 Montpellier, France). Advances in solid state fermentation : Proceedings of the 2nd International Symposium on Solid State Fermentation, FMS-95, Montpellier, France. Dordrecht : Kluwer Academic Publishers, 1997.

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International Symposium on Solid State Fermentation (2nd 1995 Montpellier, France). Advances in solid state fermentation : Proceedings of the 2nd International Symposium on Solid State Fermentation, FMS-95, Montpellier, France. Dordrecht : Kluwer Academic Publishers, 1997.

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Robinson, Tim. Solid state fermentation (SSF) of dye-adsorbed agricultural residues. [S.l : The author], 2002.

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Chapitres de livres sur le sujet "Solid fermentation and submerged"

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Minjares-Carranco, A., G. Viniegra-González et C. Augur. « Comparative studies of pectinase production by Aspergillus niger in solid state and submerged fermentations ». Dans Advances in Solid State Fermentation, 347–53. Dordrecht : Springer Netherlands, 1997. http://dx.doi.org/10.1007/978-94-017-0661-2_28.

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Agosin, E., M. Cotoras, G. Muñoz, R. San Martin et D. Volpe. « Comparative properties of Trichoderma harzianum spore produced under solid state and submerged culture conditions ». Dans Advances in Solid State Fermentation, 463–73. Dordrecht : Springer Netherlands, 1997. http://dx.doi.org/10.1007/978-94-017-0661-2_38.

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Dasari, Prabhakara Rao, Pramod W. Ramteke, Satyam Kesri et Prasada Rao Kongala. « Comparative Study of Cellulase Production Using Submerged and Solid-State Fermentation ». Dans Fungal Biology, 37–52. Cham : Springer International Publishing, 2019. http://dx.doi.org/10.1007/978-3-030-14726-6_3.

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Shoda, Makoto. « Optimization Study of Production of Antifungal Substances and Spores in Submerged Fermentation (SmF) or in Solid-State Fermentation (SSF) ». Dans Biocontrol of Plant Diseases by Bacillus subtilis, 181–262. Boca Raton, Florida : CRC Press, 2019. | : CRC Press, 2019. http://dx.doi.org/10.1201/9780429027635-7.

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Chahande, Anup D., Vidyadhar V. Gedam, Pranay A. Raut et Yogesh P. Moharkar. « Pretreatment and Production of Bioethanol from Citrus reticulata Fruit Waste with Baker’s Yeast by Solid-State and Submerged Fermentation ». Dans Utilization and Management of Bioresources, 135–41. Singapore : Springer Singapore, 2017. http://dx.doi.org/10.1007/978-981-10-5349-8_13.

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Tolan, Jeffrey S., et Brian Foody. « Cellulase from Submerged Fermentation ». Dans Recent Progress in Bioconversion of Lignocellulosics, 41–67. Berlin, Heidelberg : Springer Berlin Heidelberg, 1999. http://dx.doi.org/10.1007/3-540-49194-5_3.

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Stowell, Larry J. « Submerged Fermentation of Biological Herbicides ». Dans Microbial Control of Weeds, 225–61. Boston, MA : Springer US, 1991. http://dx.doi.org/10.1007/978-1-4615-9680-6_13.

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Sánchez, Óscar J., Sandra Montoya et Liliana M. Vargas. « Polysaccharide Production by Submerged Fermentation ». Dans Polysaccharides, 1–19. Cham : Springer International Publishing, 2014. http://dx.doi.org/10.1007/978-3-319-03751-6_39-1.

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Sánchez, Óscar J., Sandra Montoya et Liliana M. Vargas. « Polysaccharide Production by Submerged Fermentation ». Dans Polysaccharides, 451–73. Cham : Springer International Publishing, 2015. http://dx.doi.org/10.1007/978-3-319-16298-0_39.

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Alves-Prado, Heloiza Ferreira, Eleni Gomes et Roberto Da Silva. « Evaluation of Solid and Submerged Fermentations for the Production of Cyclodextrin Glycosyltransferase by Paenibacillus campinasensis H69-3 and Characterization of Crude Enzyme ». Dans Twenty-Seventh Symposium on Biotechnology for Fuels and Chemicals, 234–46. Totowa, NJ : Humana Press, 2006. http://dx.doi.org/10.1007/978-1-59745-268-7_18.

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Actes de conférences sur le sujet "Solid fermentation and submerged"

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Hermansyah, Heri, Mohammad Iqbal Andikoputro et Afrah Alatas. « Production of lipase enzyme from Rhizopus oryzae by solid state fermentation and submerged fermentation using wheat bran as substrate ». Dans THE 11TH REGIONAL CONFERENCE ON CHEMICAL ENGINEERING (RCChE 2018). Author(s), 2019. http://dx.doi.org/10.1063/1.5094991.

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Nurhayati, Nurhayati, Jayus Jayus et Hidayatul Fijriyah. « Physico-Chemical and Functional Characteristics of Fermented Cassava Flour by Lactobacillus Casei Using Submerged and Solid-State Fermentation ». Dans Proceedings of the International Conference on Food, Agriculture and Natural Resources (FANRes 2018). Paris, France : Atlantis Press, 2018. http://dx.doi.org/10.2991/fanres-18.2018.41.

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Panfilov, Victor. « SUBMERGED FERMENTATION OF JERUSALEM ARTICHOKE PULP AND EXTRACT BY LACTOBACILLUS ». Dans 17th International Multidisciplinary Scientific GeoConference SGEM2017. Stef92 Technology, 2017. http://dx.doi.org/10.5593/sgem2017/61/s25.139.

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Karetkin, Boris. « OPTIMIZATION OF WHEAT FLOUR ENZYMATIC HYDROLYSIS FOR LACTOBACILLUS RHAMNOSUS SUBMERGED FERMENTATION ». Dans 17th International Multidisciplinary Scientific GeoConference SGEM2017. Stef92 Technology, 2017. http://dx.doi.org/10.5593/sgem2017h/63/s25.039.

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Du, Linna, Jing Yang, Lili Guan, Zhongliang Liu, Xiaona Yu, Fawei Wang, Yanfang Wang, Haiyan Li et Linyu Du. « Paecilomyces tenuipes N45 submerged fermentation condition optimization using artificial neural network-genetic algorithm ». Dans The 2015 11th International Conference on Natural Computation. IEEE, 2015. http://dx.doi.org/10.1109/icnc.2015.7378166.

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« Screening of Phytase Producer Microorganisms and Optimization of Growth Conditions in Submerged Fermentation ». Dans 2014 ASABE Annual International Meeting. American Society of Agricultural and Biological Engineers, 2014. http://dx.doi.org/10.13031/aim.20141896452.

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Ong, L. G. A., H. Y. Sim et Y. S. Yong. « Biosynthesis of schizophyllan via solid state fermentation ». Dans GREEN DESIGN AND MANUFACTURE : ADVANCED AND EMERGING APPLICATIONS : Proceedings of the 4th International Conference on Green Design and Manufacture 2018. Author(s), 2018. http://dx.doi.org/10.1063/1.5066860.

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Almeida, F. C. G., C. I. M. Lins, A. M. Vieira, C. J. Vilar, M. C. Mota Lins, G. M. Campos-Takaki et E. B. Tambourgi. « Biosurfactant production by Pantoea sp in submerged fermentation using pineapple peel as an alternative medium ». Dans MICROBES IN APPLIED RESEARCH - Current Advances and Challenges. WORLD SCIENTIFIC, 2012. http://dx.doi.org/10.1142/9789814405041_0070.

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SILVEIRA, C. L. da, M. A. MAZUTTI et N. P. G. SALAU. « SOLID-STATE FERMENTATION MODEL FOR A PACKED-BED BIOREACTOR ». Dans XX Congresso Brasileiro de Engenharia Química. São Paulo : Editora Edgard Blücher, 2015. http://dx.doi.org/10.5151/chemeng-cobeq2014-1963-16649-161342.

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Silveira, Christian, Nina Salau et Marcio Mazutti. « DISTRIBUTED PARAMETER MODEL : A SOLID-STATE FERMENTATION PROCESS CASE STUDY ». Dans Simpósio Nacional de Bioprocessos e Simpósio de Hidrólise Enzimática de Biomassa. Campinas - SP, Brazil : Galoá, 2015. http://dx.doi.org/10.17648/sinaferm-2015-33530.

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Rapports d'organisations sur le sujet "Solid fermentation and submerged"

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de Vrije, Truus, et Pieternel A. M. Claassen. Production of butanol and hydrogen by fermentation techniques using steam treated municipal solid wastes : EU BESTF2 MSWBH. Wageningen : Wageningen Food & Biobased Research, 2018. http://dx.doi.org/10.18174/463764.

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Gaunaurd, Guillermo C., et Michael F. Werby. Theoretical Analogies between (Generalized) Lamb and Rayleigh Waves on Insonified, Submerged, Elastic, Hollow and Solid Curved Bodies. Fort Belvoir, VA : Defense Technical Information Center, décembre 1990. http://dx.doi.org/10.21236/ada237977.

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