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Articles de revues sur le sujet « Standard Stained Shingle Co »

Auteur : Grafiati

Publié le 25 juillet 2024

Mis à jour le 31 juillet 2025

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1

Quddus, Iram, Farhana Samir, Humera Waqar, and Sarwer Qureshi. "Concomitant Use Of L-arginine With High Butter And Corn Oil Diet Prevent Their Harmful Effects On Adrenocortical Cells Of Albino Rats." Journal of Bahria University Medical and Dental College 08, no. 01 (2017): 35–39. http://dx.doi.org/10.51985/jbumdc2018009.

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Objective: To compare the bichemical and morphological effects of L -Arginine against the changes caused by butter and corn oil supplementation Study design: A prospective experimental study Place: Department of Anatomy BMSI, JPMC Duration: August to October 2008. Methodology:Male Albino rats weighing 200 - 240gm were selected and divided into 5 groups. Group ‘CL’ received standard laboratory diet. Group ‘Bu’ received 20% added unsalted butter in diet. Group ‘Co’ received 20% added corn oil in diet. Group ‘BuAr’ received 20% Butter with L-Arginine 300mg /kg body weight /day orally .Group‘CoAr’

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Monge, Florencia A., Adeline M. Fanni, Patrick L. Donabedian, et al. "Selective In Vitro and Ex Vivo Staining of Brain Neurofibrillary Tangles and Amyloid Plaques by Novel Ethylene Ethynylene-Based Optical Sensors." Biosensors 13, no. 2 (2023): 151. http://dx.doi.org/10.3390/bios13020151.

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The identification of protein aggregates as biomarkers for neurodegeneration is an area of interest for disease diagnosis and treatment development. In this work, we present novel super luminescent conjugated polyelectrolyte molecules as ex vivo sensors for tau-paired helical filaments (PHFs) and amyloid-β (Aβ) plaques. We evaluated the use of two oligo-p-phenylene ethynylenes (OPEs), anionic OPE12- and cationic OPE24+, as stains for fibrillar protein pathology in brain sections of transgenic mouse (rTg4510) and rat (TgF344-AD) models of Alzheimer’s disease (AD) tauopathy, and post-mortem brai

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Tantisatirapong, Suchada, and Wongsakorn Preedanan. "Texture Based Classification of Malaria Parasites from Giemsa-Stained Thin Blood Films." ECTI Transactions on Electrical Engineering, Electronics, and Communications 18, no. 1 (2020): 9–16. http://dx.doi.org/10.37936/ecti-eec.2020181.208115.

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Quantification of parasitemia is an important part of a microscopic malaria diagnosis. Giemsa-stained thin blood smear is the gold standard method for detecting malaria parasite enumeration. However, manual counting reveals the limitations of human inconsistency and fatigue, as well as the unreliability of accuracy and non-reproducibility. Inaccurate parasitemia affects clinical diagnosis and therapeutic procedure. Automated quantification is therefore useful to improve the performance of quantifying parasite density. In this paper, the texture-based classification approach is investigated. Th

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Hwang, Kevin, Grace Vezeau, Edyta Olejnik, et al. "Abstract 3767: A novel method to minimize HIER-induced alterations on H&E staining in an integrated mIF-H&E workflow." Cancer Research 84, no. 6_Supplement (2024): 3767. http://dx.doi.org/10.1158/1538-7445.am2024-3767.

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Abstract Background: Hematoxylin and eosin staining (H&E) is widely used as an anatomical assay for clinical diagnosis. Researchers and clinicians also rely on molecular in situ techniques, such as multiplexed immunofluorescence (mIF), to gain deeper insights on cellular phenotypes and tissue microenvironment. As a result, there has been significant interest in combining anatomical stains with molecular imaging techniques, most commonly by using a terminal H&E stain after mIF staining. This allows a combination of the two assays but has been observed to show alterations in the H&E

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Alatrash, Gheath, Pariya Sukhumalchandra, Mao Zhang, et al. "PR1 Is Cross-Presented By Multiple Myeloma Cells in Patients and Renders Multiple Myeloma Susceptible to PR1-CTL and Anti-PR1/HLA-A2 Antibody." Blood 124, no. 21 (2014): 2133. http://dx.doi.org/10.1182/blood.v124.21.2133.2133.

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Abstract PR1 is an HLA-A2-retricted, nonameric peptide that is derived from the azurophil granule proteases neutrophil elastase (NE) and proteinase 3 (P3). PR1 has been targeted successfully in acute (AML) and chronic (CML) myeloid leukemia using anti-PR1/HLA-A2 antibody (8F4), PR1-peptide vaccine and PR1-specific cytotoxic T lymphocytes (PR1-CTL). We have previously reported that NE and P3 are cross-presented by normal B cells and dendritic cells (DC), leading to PR1 expression by HLA-A2. Since multiple myeloma (MM) is a B cell malignancy, we investigated whether MM cells can cross-present PR

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Nikolskiy, I., V. Nikolskaya, D. Demchenko, and D. Zubov. "Potentiation of directed osteogenic differentiation of thymic multipotent stromal cells by prior co-cultivation with thymocytes." Cell and Organ Transplantology 4, no. 2 (2016): 220–23. http://dx.doi.org/10.22494/cot.v4i2.59.

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It is known that multipotent stromal cells (MSCs) and thymocytes possess membrane affinity and interaction in the thymic niches that is essentially important for thymocytes differentiation. However there are no data about possible influence of intercellular contacts in the reverse direction: from the thymocytes to the MSCs.Materials and methods. The MSCs were obtained from the thymuses of С57ВL mice, using the explants technique, and cultivated under standard conditions during 8-12 passages. Thymocytes or bone marrow cells (106) were added to 4×104 MSCs for 24 hours. Thereafter they were elimi

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DOONABOYINA, RAGHAVA, Abhilasha Mittal, and Sridhar Babu Gummadi. "IN VITRO SULFORHODAMINE B ASSAY EVALUATION OF NOVEL 2-PHENYL BENZOFURANONE DERIVATIVES ON HUMAN SKIN CANCER CELL LINE G361." Journal of Drug Delivery and Therapeutics 9, no. 4-A (2019): 385–89. http://dx.doi.org/10.22270/jddt.v9i4-a.3434.

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The newly synthesized compounds are being tested for in vitro anticancer activity. The method used for In Vitro testing is Sulforhodamine B assay also known SRB assay. Cell lines were prepared and homogenized and disassociated with the help of trypsin. Then trypsin was inactivated with fetal bovine serum. Then cell concentration was determined. Synthesized molecules were prepared into four different dilutions and exposed to cell lines. The procedure was also compared with standard drug doxorubicin. All the cell medium were incubated 37 degrees centigrade in a humidified incubator with 5 percen

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Aertker, Benjamin M., Akshita Kumar, Fanni Cardenas, et al. "PET Imaging of Peripheral Benzodiazepine Receptor Standard Uptake Value Increases After Controlled Cortical Impact, a Rodent Model of Traumatic Brain Injury." ASN Neuro 13 (January 2021): 175909142110141. http://dx.doi.org/10.1177/17590914211014135.

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Traumatic brain injury (TBI) is a chronic, life threatening injury for which few effective interventions are available. Evidence in animal models suggests un-checked immune activation may contribute to the pathophysiology. Changes in regional density of active brain microglia can be quantified in vivo with positron emission topography (PET) with the relatively selective radiotracer, peripheral benzodiazepine receptor 28 (11 C-PBR28). Phenotypic assessment (activated vs resting) can subsequently be assessed (ex vivo) using morphological techniques. To elucidate the mechanistic contribution of i

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Thongheang, Kanyarat, Thanathat Pamonsupornwichit, Kanokporn Sornsuwan, et al. "Potentiating Antibody-Dependent Cellular Cytotoxicity in Triple-Negative Breast Cancer via the Humanized Anti-CD147 Antibody." Antibodies 14, no. 2 (2025): 36. https://doi.org/10.3390/antib14020036.

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Background: Triple-negative breast cancer (TNBC) is an aggressive subtype with high metastatic potential, poor prognosis, and the absence of estrogen receptors, progesterone receptors, and human epidermal growth factor receptor 2 (HER2). The lack of these receptors limits the standard treatments, such as hormone therapies and HER2-targeted antibodies like trastuzumab. These challenges highlight the critical need for novel therapeutic strategies. CD147, a transmembrane glycoprotein overexpressed in TNBC, promotes tumor progression, metastasis, and chemoresistance, making it a promising therapeu

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Pantic, Igor, Sanja Dacic, Predrag Brkic, et al. "Application of Fractal and Grey Level Co-Occurrence Matrix Analysis in Evaluation of Brain Corpus Callosum and Cingulum Architecture." Microscopy and Microanalysis 20, no. 5 (2014): 1373–81. http://dx.doi.org/10.1017/s1431927614012811.

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AbstractThis aim of this study was to assess the discriminatory value of fractal and grey level co-occurrence matrix (GLCM) analysis methods in standard microscopy analysis of two histologically similar brain white mass regions that have different nerve fiber orientation. A total of 160 digital micrographs of thionine-stained rat brain white mass were acquired using a Pro-MicroScan DEM-200 instrument. Eighty micrographs from the anterior corpus callosum and eighty from the anterior cingulum areas of the brain were analyzed. The micrographs were evaluated using the National Institutes of Health

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Szabolcs, M. J., A. Windisch, R. Koller, and M. Pensch. "Axon typing of rat muscle nerves using a double staining procedure for cholinesterase and carbonic anhydrase." Journal of Histochemistry & Cytochemistry 39, no. 12 (1991): 1617–25. http://dx.doi.org/10.1177/39.12.1719070.

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We developed a method for detecting activity of axonal cholinesterase (CE) and carbonic anhydrase (CA)--markers for motor and sensory nerve fibers (NFs)--in the same histological section. To reach this goal, cross-sections of muscle nerves were sequentially incubated with the standard protocols for CE and CA histochemistry. A modified incubation medium was used for CA in which Co++ is replaced by Ni++. This avoids interference of the two histochemical reactions because Co++ binds unspecifically to the brown copper-ferroferricyanide complex representing CE activity, whereas Ni++ does not. Cross

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Kobayashi, Hirofumi, Michael E. Kallen, Raymond Kozikowski, et al. "Real-time, multiplexed rendering of lymphoma diagnostic panels from unstained tissue sections using virtual staining." JCO Global Oncology 9, Supplement_1 (2023): 115. http://dx.doi.org/10.1200/go.2023.9.supplement_1.115.

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115 Background: Lymphoproliferative disorders are complex, heterogeneous diseases with an ever expanding array of subtypes and variants requiring stepwise assay approach to arrive at a precise diagnosis. While extensive characterization of lesional cells and the microenvironment (TME) via immunophenotyping and genotyping remains the gold standard, a full workup may be unachievable due to limited input tissue, turnaround time pressure, or unavailability of particular markers in-house. Even when available, the interpretation of large numbers of markers by pathologists is limited by the ability t

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Naderi, Misagh, Nikola Mitic, Nikola Spasic, et al. "Abstract 7391: Beyond traditional staining: A virtual-IHC digital assay for HER2 score prediction from routine H&E images." Cancer Research 84, no. 6_Supplement (2024): 7391. http://dx.doi.org/10.1158/1538-7445.am2024-7391.

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Abstract Background: In breast cancer diagnostics, the accurate assessment of HER2 status is essential for treatment stratification. Conventionally, HER2 immunohistochemistry (IHC) scoring necessitates specialized staining protocols, which are resource- and time-intensive. This study presents a virtual IHC (vIHC) digital assay to predict HER2 scores directly from hematoxylin and eosin (H&E) stained images, which are routinely acquired for all specimens. This methodology aims to accelerate standard practices by reducing the need for additional IHC staining, thereby decreasing laboratory wor

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Downing, Michael, Ruben Cardenes, Douglas Wood, Gourab Chatterjee, and Lorenz Rognoni. "Abstract 6310: Bridging spatial proteomics and classical histopathology: advanced spatial analysis through H&E-enhanced multiplexed immunoprofiling." Cancer Research 85, no. 8_Supplement_1 (2025): 6310. https://doi.org/10.1158/1538-7445.am2025-6310.

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Background: Multiplex immunofluorescence (mIF) is increasingly adopted in translational research due to its ability to provide rich spatial and molecular information. Nevertheless, H&E staining remains the gold standard for clinical diagnosis, revealing essential histological context and morphological features not visible in mIF images. While H&E is routinely performed on serial sections in analytical mIF workflows, biological variability between sections limits its utility. Co-registration of H&E and mIF images from the same slide enables precise colocalization of cells across cha

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Karunakaran, Poongkodi, Sujatha Jayaraman, Sumathi Periyasamy, and Ramesh Subburaman. "The impact of Hashimoto’s thyroiditis on the diagnostic utility of P63 and CK19 immunohistochemistry markers in predicting thyroid cancer." International Surgery Journal 11, no. 12 (2024): 2040–46. http://dx.doi.org/10.18203/2349-2902.isj20243544.

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Background: The incidence of thyroid cancer (TC) and Hashimoto’s thyroiditis (HT) is increasing worldwide. Coexisting HT adds to diagnostic confusion in establishing TC on histopathological examination (HPE). Studies have shown the promising role of immunohistochemistry markers in predicting cancer, but the impact of coexisting HT is unclear. This prospective study determined the impact of HT on the diagnostic utility of p63 and CK19 in predicting thyroid cancer. Methods: Out of 103 patients undergoing total thyroidectomy for benign or malignant thyroid nodules, 31 patients (mean age=39.7 year

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Soniran, Olajoju, Dr. Segun Solomon Ogundapo, and Dr.Joseph Bagi Suleiman. "Antiplasmodial activities of selected artemisinin-based combination therapy (ACT) drugs co-administered with beer in Plasmodium berghei-infected albino rats." International Journal of Emerging Multidisciplinaries: Biomedical and Clinical Research 2, no. 1 (2024): 12. https://doi.org/10.54938/ijemdbmcr.2024.02.1.375.

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Alcoholic beverages, particularly beer, are widely consumed in Nigeria despite its contribution to most disability, deaths, and some non-communicable diseases. Based on unpublished reports, some individuals consume alcoholic beverages while treating malaria, which raises concerns about potential interactions between alcohol and antimalaria drugs. However, there is paucity of information on the effect of alcoholic beverage consumption on the management of malaria. Hence, this study investigated the effect of alcoholic beverage co-administered with Artemisinin-based Combination Therapy (ACT) dru

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Travain, Willian, Hanna Carolina Bet Dos Santos, Valéria Schoffen Romao Carrascoza, Márcia Do Nascimento Brito, and Célia Regina Godoy Gomes. "Efeito do Óleo de Coco sobre a Morfologia da Aorta de Ratos Obesos." Saúde e Pesquisa 8, no. 1 (2015): 35. http://dx.doi.org/10.17765/1983-1870.2015v8n1p35-43.

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O objetivo desse estudo foi avaliar a influência do óleo de coco sobre morfologia da aorta torácica. Foram utilizados 23 animais, com 120 dias de idade. Os animais foram inicialmente separados em dois grupos, controle (tratado com dieta padrão para roedores) e obeso (tratado com dieta hipercalórica para induzir a obesidade). Aos 90 dias, os animais dos grupos controle e obeso foram subdivididos em dois grupos adicionais: C/ag, C/co, O/ag, O/co. Aos 120 dias foram eutanasiados com pentobarbitol sódico. As aortas torácicas extraídas foram processadas histologicamente e coradas com Tricrômico de

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Lee, S. H., E. M. N. Setyawan, and B. C. Lee. "166 Paracrine Regulation of Epidermal Growth Factor-Like Factors in Oviduct Cells and its Effect on Oocyte Maturation and Subsequent Embryo Development." Reproduction, Fertility and Development 30, no. 1 (2018): 222. http://dx.doi.org/10.1071/rdv30n1ab166.

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Progesterone (P4) and progesterone receptor signalling appears essential for maintenance of a proper cumulus cell expansion during the oocyte maturation by regulating the epidermal growth factor-like factors (EGF-F) related pathway during the ovulatory process. It is known that expression of EGF-F including amphiregulin (AREG), epiregulin (EREG), and betacellulin (BTC) is critical for cumulus–oocyte complex (COC) expansion and resumption of meiosis. Therefore, we hypothesised that oviduct cells might be involved in nonexclusive mechanisms of actions of P4 that in turn modulate oocyte meiosis r

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Pokorska-Bocci, Anna, Sandrine Micallef, Mariola Dymkowska, et al. "Predicting response to naratuximab emtansine, an anti-CD37 antibody-drug conjugate (ADC), in combination with rituximab in diffuse large B-cell lymphoma (DLBCL), by analyzing the spatial arrangement of CD37 and CD20 positive cells using deep learning." Journal of Clinical Oncology 40, no. 16_suppl (2022): e19565-e19565. http://dx.doi.org/10.1200/jco.2022.40.16_suppl.e19565.

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e19565 Background: DLBCL is the most common type of Non-Hodgkin’s lymphoma, accounting for 30-40% of cases. Despite improvements in response and survival with standard of care treatment, up to 40% of patients have relapsed and/or refractory (R/R) disease. A phase 2 study (NCT02564744) evaluated the efficacy of naratuximab emtansine, an anti-CD37 ADC, in combination with rituximab, in 80 patients with R/R DLBCL. We performed an exploratory analysis of the study to find pathology-based biomarkers predictive of response. Deep learning (DL) models were used to extract spatial features from digitiz

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Khan, AR, M. Cocker, JD Spence, et al. "3D carotid reconstructions: imaging, pathology, algorithms and pipelines." Canadian Journal of Neurological Sciences / Journal Canadien des Sciences Neurologiques 42, S1 (2015): S37. http://dx.doi.org/10.1017/cjn.2015.170.

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Background: Whole-slide scanning of tissue sections spatially informed by imaging studies offers the opportunity to reconstruct specimens for co-registration to 3D imaging data. Digital image analysis algorithms can be designed to analyze and reconstruct such specimens via electronic “pipelines”. Methods: A goal of the Canadian Atherosclerosis Imaging Network (CAIN) is to improve the assessment of carotid atheromatous disease through studies that inform clinical imaging with gold-standard data (plaque pathology). To achieve this, sectioned atheromas are manually annotated and analyzed by elect

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Jovanovic-Medojevic, Milica, Vanja Opacic-Galic, Katarina Geler, and Dusan Pavlica. "Comparison of the efficiency of cleaning and disinfection protocols for hand endodontic instruments." Vojnosanitetski pregled, no. 00 (2022): 103. http://dx.doi.org/10.2298/vsp220913103j.

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Background/Aim. There is no standard protocol for cleaning and desinfection of used endodontic instruments before their sterilization and reuse. The aim of this study wss to check the efficiency of the different methods of removing biological debris from different type of used hand stainless steel endodontic instruments. Methods. A total of 120 instruments (forty Kerr-Reamers, Kerr-Files and Hedstr?m-Files) (Shenzhen Denco Medical Co., Ltd. Guangdong, China) ISO 25 were analyzed. The instruments used for root canal treatment on extracted teeth were divided into 4 groups based on different deco

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Meah, Arafat, Vadanasundari Vedarethinam, Robert Bronstein, et al. "Single-Cell Spatial MIST for Versatile, Scalable Detection of Protein Markers." Biosensors 13, no. 9 (2023): 852. http://dx.doi.org/10.3390/bios13090852.

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High-multiplex detection of protein biomarkers across tissue regions has been an attractive spatial biology approach due to significant advantages over traditional immunohistochemistry (IHC) methods. Different from most methods, spatial multiplex in situ tagging (MIST) transfers the spatial protein expression information to an ultrahigh-density, large-scale MIST array. This technique has been optimized to reach single-cell resolution by adoption of smaller array units and 30% 8-arm PEG polymer as transfer medium. Tissue cell nuclei stained with lamin B have been clearly visualized on the MIST

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Rittel, Miriam F., Stefan Schmidt, Cleo-Aron Weis, et al. "Spatial Omics Imaging of Fresh-Frozen Tissue and Routine FFPE Histopathology of a Single Cancer Needle Core Biopsy: A Freezing Device and Multimodal Workflow." Cancers 15, no. 10 (2023): 2676. http://dx.doi.org/10.3390/cancers15102676.

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The complex molecular alterations that underlie cancer pathophysiology are studied in depth with omics methods using bulk tissue extracts. For spatially resolved tissue diagnostics using needle biopsy cores, however, histopathological analysis using stained FFPE tissue and the immunohistochemistry (IHC) of a few marker proteins is currently the main clinical focus. Today, spatial omics imaging using MSI or IRI is an emerging diagnostic technology for the identification and classification of various cancer types. However, to conserve tissue-specific metabolomic states, fast, reliable, and preci

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Li, Pengfei, Hongli Luo, Yanhui Chen, et al. "Immune biomarker expression in the tumor microenvironment in Chinese patients with esophageal squamous cell carcinoma was explored." Journal of Clinical Oncology 37, no. 15_suppl (2019): e15542-e15542. http://dx.doi.org/10.1200/jco.2019.37.15_suppl.e15542.

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e15542 Background: Esophageal squamous cell carcinoma (ESCC) is one of the most common malignancies in China, of which the standard treatment of advanced esophageal cancer is chemotherapy followed by surgery. Recently, immunotherapy has shown great potential in ESCC treatment. The aim of this study was to explore the landscape of immune-related gene expression in the tumor microenvironment (TIME) and further explore the relationship between immune biomarkers and therapeutic effect. Methods: We retrospectively analyzed PDL1(clone sp142)/PD1 expression in a cohort of Chinese patients (male = 92,

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Bhattarai, Shristi, Geetanjali Saini, Hongxiao Li, et al. "Predicting Neoadjuvant Treatment Response in Triple-Negative Breast Cancer Using Machine Learning." Diagnostics 14, no. 1 (2023): 74. http://dx.doi.org/10.3390/diagnostics14010074.

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Background: Neoadjuvant chemotherapy (NAC) is the standard treatment for early-stage triple negative breast cancer (TNBC). The primary endpoint of NAC is a pathological complete response (pCR). NAC results in pCR in only 30–40% of TNBC patients. Tumor-infiltrating lymphocytes (TILs), Ki67 and phosphohistone H3 (pH3) are a few known biomarkers to predict NAC response. Currently, systematic evaluation of the combined value of these biomarkers in predicting NAC response is lacking. In this study, the predictive value of markers derived from H&E and IHC stained biopsy tissue was comprehensivel

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Yeo, L., N. Adlard, M. Biehl, et al. "Expression of chemokines CXCL4 and CXCL7 by synovial macrophages defines an early stage of rheumatoid arthritis." Annals of the Rheumatic Diseases 75, no. 4 (2015): 763–71. http://dx.doi.org/10.1136/annrheumdis-2014-206921.

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Background and objectivesFor our understanding of the pathogenesis of rheumatoid arthritis (RA), it is important to elucidate the mechanisms underlying early stages of synovitis. Here, synovial cytokine production was investigated in patients with very early arthritis.MethodsSynovial biopsies were obtained from patients with at least one clinically swollen joint within 12 weeks of symptom onset. At an 18-month follow-up visit, patients who went on to develop RA, or whose arthritis spontaneously resolved, were identified. Biopsies were also obtained from patients with RA with longer symptom dur

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Sartain, Sarah E., Nancy A. Turner, Hui Shiu-Ki, Charles G. Minard, and Joel L. Moake. "Factor H (FH) Is Released Slowly and Continuously from Human Endothelial Cells (ECs): FH Is Not Packaged in Weibel-Palade Bodies or Secreted in Response to EC Stimulation." Blood 124, no. 21 (2014): 4175. http://dx.doi.org/10.1182/blood.v124.21.4175.4175.

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Abstract Introduction Ultra-large von Willebrand factor (ULVWF) strings are synthesized in ECs, packaged in Weibel-Palade Bodies (WPBs), and secreted by stimulated ECs. Complement components studied to date [C3, factor (F) B, FD, FP, FH, FI, C5] are released slowly and continuously from human umbilical vein endothelial cell (HUVEC) cytoplasm and are not packaged in WPBs (PLoS One. 2013;8(3):e59372). In contrast, a recent report (Blood. 2014;123(1):121-5) contended that FH co-localizes with VWF in the WPBs. If this were so, it could have therapeutic importance for the treatment of atypical hemo

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Takahashi, Hiromichi, Sumiko Kobayashi, Katsuhiro Miura, et al. "Clinical Significance of Co-Expression of MYC and BCL2 Protein in Advanced Diffuse Large B-Cell Lymphoma Treated with a Dose-Intensified Immunochemotherapy." Blood 126, no. 23 (2015): 3940. http://dx.doi.org/10.1182/blood.v126.23.3940.3940.

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Abstract Background Recent studies have shown that the concurrent expression of MYC and BCL2 protein evaluated by immunohistochemistry (IHC) in patients with de novo diffuse large B-cell lymphoma (DLBCL) is associated with worse survival when treated with standard R-CHOP, but the effect of intensive chemotherapies for such patients is unknown. Thus, we evaluated the impact of the co-expression of MYC and BCL2 protein among patients with advanced DLBCL, who were treated with a dose-intensive immunochemotherapy followed by up-front autologous stem cell transplantation (ASCT). Patients and Method

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Sefland, Oystein, Reidun Kristin Kopperud, Hester Van Zeeburg, et al. "Intradermal Vaccination with Vididencel in MRD+ AML-Patients Leads to Increase in Antigen Presenting Cells and T-Cells to the Injection Site, Visualized Using Imaging Mass Cytometry, Showing Local Immune Cell Interactions Leading to Systemic Immune Responses." Blood 142, Supplement 1 (2023): 2942. http://dx.doi.org/10.1182/blood-2023-180813.

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Background. Vididencel is an allogeneic leukemia-derived dendritic cell vaccine being developed as maintenance treatment in AML patients. Intradermal vaccination using vididencel is known to induce a local skin reaction, with local antigen processing by skin dendritic cells and direct presentation to T cells as well as dendritic cells, crawling out of the skin and presentation of the antigen in the draining lymph nodes for a full-blown immune response. It was previously shown that vididencel is able to induce an influx of immune cells in the skin using standard immunohistochemistry (van de Loo

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Cherepanova, N. G., K. I. Novicova, A. A. Agarkova, N. P. Belyaeva, and E. A. Prosekova. "Tinctorial properties of a non-meat proteins." Proceedings of the Voronezh State University of Engineering Technologies 86, no. 2 (2024): 33–39. https://doi.org/10.20914/2310-1202-2024-2-33-39.

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The article discusses the identification of vegetable protein additives used in the meat industry using histological methods. The supplements tested were Soy Protein Isolate Powder 90%, Pea Protein Powder 80%, and Rice Protein Powder 80%, which were provided by Summit Ingredients Co., Ltd. (China). Samples of minced chicken breast were prepared; 0.2 g of dry additive was added to 10 g of minced meat. Histological samples were prepared using standard methods. Staining was carried out with different groups of dyes specified in regulatory documents. Each sample was stained with the following dyes

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Bali, Ayman, Saskia Wolter, Daniela Pelzel, et al. "Real-Time Intraoperative Decision-Making in Head and Neck Tumor Surgery: A Histopathologically Grounded Hyperspectral Imaging and Deep Learning Approach." Cancers 17, no. 10 (2025): 1617. https://doi.org/10.3390/cancers17101617.

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Background: Accurate and rapid intraoperative tumor margin assessment remains a major challenge in surgical oncology. Current gold-standard methods, such as frozen section histology, are time-consuming, operator-dependent, and prone to misclassification, which limits their clinical utility. Objective: To develop and evaluate a novel hyperspectral imaging (HSI) workflow that integrates deep learning with three-dimensional (3D) tumor modeling for real-time, label-free tumor margin delineation in head and neck squamous cell carcinoma (HNSCC). Methods: Freshly resected HNSCC samples were snap-froz

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Lee, S. H., H. J. Oh, M. J. Kim, et al. "174 EFFECT OF HUMAN ENDOTHELIAL PROGENITOR CELLS ON IN VITRO MATURATION OF PORCINE OOCYTES AND PARTHENOGENETIC EMBRYO DEVELOPMENT COMPETENCE." Reproduction, Fertility and Development 29, no. 1 (2017): 195. http://dx.doi.org/10.1071/rdv29n1ab174.

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In oocyte maturation, hepatocyte growth factor and vascular endothelial growth factor (VEGF) contribute to promote granulosa cell proliferation and cumulus cell expansion. It is well known that human endothelial progenitor cells (hEPC), which are isolated from monocytes and macrophages, secrete a variety of growth factors, such as hepatocyte growth factor and VEGF, and improve the process of angiogenesis. Therefore, the aim of this study was to investigate the effects of hEPC on in vitro oocyte maturation and subsequent embryo development in pigs. To isolate and culture hEPC, human peripheral

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Bassi, Giulio, Luciano Pacelli, Cedric Mènard, et al. "Microporous Biphasic Calcium Phosphate Granules (MBCP®) Retain Immunological Properties of Bone Marrow-Derived Mesenchymal Stromal Cells and Promote Osteoblastic Differentiation." Blood 118, no. 21 (2011): 1924. http://dx.doi.org/10.1182/blood.v118.21.1924.1924.

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Abstract Abstract 1924 Bone is among the most frequently transplanted tissue with about 1 million procedures annually in Europe. Allografts and autografts account for more than 80% of total graft volume, despite their considerable disadvantages, including the risk of disease transfer and immunologic rejection, limited supply of bone, costs and complications. Significant growth opportunities exist for synthetic bone grafts in association with mesenchymal stromal cells (MSC) from autologous or allogeneic sources as alternatives to biological bone grafts in orthopaedic and maxillofacial surgery.

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Dwina, Yayi, Ria Kodariah та Endang S. R. Hardjolukito. "Identification of pathogenesis pathway in basal-like breast cancer based on mutant p53 protein and topoisomerase-IIα expression". Medical Journal of Indonesia 23, № 4 (2015): 197–202. http://dx.doi.org/10.13181/mji.v23i4.995.

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Background: Basal-like breast cancer is difficult to treat with standard regimen therapy, because it doesn’t express hormone receptors or epidermal growth factor receptors. Identification of oncogenesis pathway is expected to find molecules which can be used as target for therapy. One candidate molecule is topoisomerase-IIα whose expression is regulated by p53. This study aimed to compare the expression of mutant p53 proteins and topoisomerase IIα in basal-like and non basal-like breast cancer, and to determine the association between mutant p53 proteins and topoisomerase IIα in basal-like gro

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Demyda-Peyrás, S., M. Hidalgo, J. Dorado, and M. Moreno-Millan. "87 EVALUATION OF THE NUMERICAL CHROMOSOMAL ABNORMALITIES ON IN VITRO EARLY BOVINE EMBRYOS: EFFECT OF THE CELL CO-CULTURE WITH GRANULOSA CELLS." Reproduction, Fertility and Development 26, no. 1 (2014): 157. http://dx.doi.org/10.1071/rdv26n1ab87.

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Chromosomal numerical abnormalities (CNA) were described as a major cause of developmental failures in in vitro-produced (IVP) embryos. It has been described that CNA are influenced by the post-fertilization culture environment of the embryo. Furthermore, it was demonstrated that the use of different culture media affects the CNA rates. The addition of granulosa cells during early embryo development is a well-known procedure to simplify the culture of bovine IVP and cloned embryos. This technique avoids the use of culture environments saturated with N2 (tri-gas chambers). The aim of this study

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Nwarunma, Ebubechukwu, Katerina Miari, Athanasia Papadopoulou, et al. "M2-like Macrophages Transfer Mitochondria to Acute Myeloid Leukaemia Cells Via Tunnelling Nanotubes Promoting Therapy Resistance." Blood 144, Supplement 1 (2024): 2761. https://doi.org/10.1182/blood-2024-200028.

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Introduction: The bone marrow microenvironment actively drives therapy resistance in AML. Leukaemic cells interact with bone marrow (BM) resident stromal cells and immune cells, such as macrophages (Mφs). M2-like Mφs are elevated in the BM of AML patients, and transfer their mitochondria to primary AML cells enhancing AML cell proliferation (Weinhäuser et al. Science Advances 2022). Mitochondrial transfer from BM stromal cells (BMSCs) to AML cells is increased by cytarabine (Ara-C), with transfer driving BMSC-mediated protection from ara-C-induced apoptosis (Moschoi et al. Blood 2016). We have

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Thaweboon, Sroisiri, Passiri Nisalak, Boonyanit Thaweboon, Pornrachanee Sawaengkit, Plang Ngern Saksit, and Rattiporn Kaypetch. "Antimicrobial Activity of Type III Dental Gypsum Incorporated with 3-iodo-2-propynyl-butylcarbamate and its Physical Properties." Advanced Materials Research 1052 (October 2014): 322–26. http://dx.doi.org/10.4028/www.scientific.net/amr.1052.322.

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Dental impressions have been considered to be potential sources of microbial contamination from patients’ blood and saliva to dental personnel and prostheses fabricated on gypsum casts. Thus, the development of dental gypsum with antimicrobial activity to reduce cross-contamination between patients and laboratory personnel is needed. This study aims to evaluate the influence of incorporation of 3-iodo-2-propynyl-butylcarbamate (IPBC) into type III dental gypsum on its antimicrobial activities and physical properties such as dry compressive strength and setting time. Type III dental gypsum (The

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Gozhenko, A., O. Bestanchuk, O. Kaschenko, and T. Narbutova. "Cumulative cardiotoxic effect of bleomycin in experiment." Journal of Education, Health and Sport 11, no. 6 (2021): 301–8. http://dx.doi.org/10.12775/jehs.2021.11.06.033.

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The aim of the study was to evaluate the cumulative toxic effect of bleomycin under experimental conditions Material and Methods. The study was conducted in the Research Institute of Transport Medicine during 2016-2021. The experimental model of the cardiotoxic effect of the bleomycin was performed using the medication "Bleocin" manufactured by Nippon Kayaku Co., Ltd. (Japan). According to the task, the study was performed on 10 mature rats of both sexes of the Wistar line with a body weight of 237 ± 20 g. Rats were housed in standard vivarium conditions of Odessa National Medical

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Kurmi, Roshan, Ramanuj Rauniyar, Krishna Das Manandhar, and Birendra Prasad Gupta. "Evaluation of the XpertMTB/RIF for the Diagnosis of Pulmonary Tuberculosis Among the Patients Attending DOTS Center Parsa District of Nepal." Nepal Journal of Biotechnology 4, no. 1 (2016): 26–32. http://dx.doi.org/10.3126/njb.v4i1.16343.

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Tuberculosis diagnosis and monitoring rely in Sputum microscopy of National Tuberculosis Programme, Nepal because of its low cost and easier to perform. Direct sputum microscopy is popular worldwide. Currently, there are 533 microscopy centres catering for sputum microscopy examination throughout the country. Most of the microscopy centres are established within government jurisdiction and remaining are established as non-governmental organization as well as private sectors.A cross-sectional study was conducted from July 2013 to January 2015. A total of 2091 patients were enrolled in the study

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Lim, Ki-Byung, Jannie Wennekes, J. Hans de Jong, Evert Jacobsen, and Jaap M. van Tuyl. "Karyotype analysis of Lilium longiflorum and Lilium rubellum by chromosome banding and fluorescence in situ hybridisation." Genome 44, no. 5 (2001): 911–18. http://dx.doi.org/10.1139/g01-066.

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Detailed karyotypes of Lilium longiflorum and L. rubellum were constructed on the basis of chromosome arm lengths, C-banding, AgNO3 staining, and PI-DAPI banding, together with fluorescence in situ hybridisation (FISH) with the 5S and 45S rDNA sequences as probes. The C-banding patterns that were obtained with the standard BSG technique revealed only few minor bands on heterologous positions of the L. longiflorum and L. rubellum chromosomes. FISH of the 5S and 45S rDNA probes on L. longiflorum metaphase complements showed overlapping signals at proximal positions of the short arms of chromosom

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Sharma, Shilpa, Renata Pereira, Elizabeta Nemeth, et al. "Serum Ferritin Reflects Bone Marrow Iron Stores in Children with End Stage Kidney Disease." Blood 142, Supplement 1 (2023): 1090. http://dx.doi.org/10.1182/blood-2023-182483.

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Background: Iron deficiency is common in children with end-stage kidney disease (ESKD) but diagnosing iron deficiency in ESKD is challenging as serum indicators of iron stores may be influenced by inflammation and other factors. We examined which of the clinically used serum iron indices were predictive of bone marrow iron stores (the gold standard) in dialysis-dependent children and young adults with ESKD, in the setting of increased nutritional requirements and co-existing inflammation. Methods: This cross-sectional study enrolled 71 clinically-stable children and young adults (age range 2 t

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Gibson, Amber, Pariya Sukhumalchandra, Celine Kerros, et al. "Abstract 2995: Cross-presented serine proteases as immunotherapy targets for lung cancer and osteosarcoma." Cancer Research 83, no. 7_Supplement (2023): 2995. http://dx.doi.org/10.1158/1538-7445.am2023-2995.

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Abstract Background: Immunotherapy is the standard of care for many solid tumor malignancies. However, due to a paucity of known tumor antigens in non-small cell lung cancer (NSCLC) and osteosarcoma (OS), identification of novel immunogenic antigens is needed. Our group has experience with neutrophil serine proteases (NSP) derived from the azurophilic granules of polymorphonuclear leukocytes (PMN), specifically, cathepsin (CG), proteinase 3 (P3), and neutrophil elastase (NE). We have identified two HLA-A2 (HLA-A*0201)-restricted nonameric peptides: CG1 (FLLPTGAEA), derived from CG, and PR1 (VL

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Sewerin, P., L. Dötsch, D. Truhm, D. Abrar, and S. Nebelung. "THU0062 FUNCTIONAL MR IMAGING OF HUMAN MENISCUS IS ASSOCIATED WITH HISTOLOGICAL DEGENERATION." Annals of the Rheumatic Diseases 79, Suppl 1 (2020): 243. http://dx.doi.org/10.1136/annrheumdis-2020-eular.6403.

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Background:In OA, there is a close association of meniscus and cartilage pathologies. Meniscus degeneration and lesioning are critical risk factors for development of early OA. Hence, thisex-vivostudy assessed the responses to standardized loading of human meniscus samples as a function of degeneration and based on changes in their T1, T2 and T1ρ maps (as surrogate parameters of the tissue’s functionality).Objectives:Can meniscus functionality be visualized by serial quantitative MRI mapping technics?Methods:During total knee replacements, 45 meniscus samples of variable degeneration were harv

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Zakharov, Oleg D., Ekaterina U. Rybalkina, Alla A. Stavrovskaya, and Maya Volkova. "Prognostic Significance of Multidrug Resistance Proteins Expression in Acute Myeloid Leukemia." Blood 108, no. 11 (2006): 4360. http://dx.doi.org/10.1182/blood.v108.11.4360.4360.

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Abstract Background: Conventional induction chemotherapy induces complete remission (CR) in 65–75% of adults with de novo acute myeloid leukemia (AML), and 15–20% of the patients have refractory disease. We investigated the prognostic significance of multidrug resistance proteins (P-glycoprotein (Pgp), BCRP, MRP1 and LRP) expression on AML blast cells before treatment. Methods: We included in analysis 30 patients (pts) with de novo AML. Expression of multidrug resistance proteins (MDR) was detected by indirect immunofluorescence technique and flow cytometry on bone marrow blast cells before ch

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Odeh, Mary, Alice Amachree, Adetutu Olubunmi Obulor, and Eme Efioanwan Orlu. "Critical Evaluation of the Role of Lycopene on Reproduction of Male Wistar Rats Exposed to Dimethoate." Asian Journal of Biology 18, no. 2 (2023): 1–10. http://dx.doi.org/10.9734/ajob/2023/v18i2337.

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Aim: This study was aimed at evaluating the role of Lycopene on reproduction of male Wistar rats exposed to Dimethoate. Experimental Design: A completely randomized experimental design using standard methods for analysis.  Location and Duration of Study: This study was carried out in the Animal house, Department of Animal and Environmental Biology of Rivers State University, Nkpolu-Oroworukwo Port Harcourt, Nigeria. GPS 4o47'50''N 6o58'49''E. This study lasted for 21days.  Methodology: Thirty male rats were randomly selected into five (5) groups A-E (6 animals per group). Gro

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Bhumika, Baria, Agravat Amit, Dhruva Gauravi, and Pujara Krupal. "A Study on Different Techniques of Laboratory Detection of Malaria in a Tertiary Care Hospital in Western India." International Journal of Pharmaceutical and Clinical Research 16, no. 7 (2024): 1553–60. https://doi.org/10.5281/zenodo.13336763.

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<strong>Background:</strong> Malaria is one of the most prevalent parasitic infections in the world including India. It is a tropical, endemic protozoal infection. In most countries, its diagnosis is challenging. Microscopy is the gold standard method for its diagnosis in clinical practice as well as for research purpose. However, it is laborious, requiring significant skills and time, causing therapeutic delays. Rapid malaria diagnostic tests (RDTs) have made possible to obtain quick results from the blood examination. Various fast, reliable and easy to use RDTs are available which can d

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Chen, Haiming, Mingjie Li, Suzie Vardanyan, et al. "Increased M2 Macrophages and Higher Levels Of The Tribble Family Member Trib1 In Monocytes Are Associated With Progressive Disease In Multiple Myeloma (MM) Patients." Blood 122, no. 21 (2013): 3127. http://dx.doi.org/10.1182/blood.v122.21.3127.3127.

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Abstract Macrophages consist of two subgroups, M1 and M2. M1 macrophages are pro-inflammatory cells against bacterial and viral infections whereas M2 macrophages are anti-inflammatory and associated with tumor progression. The mammalian tribble (Trib) family of genes, Trib1, Trib2 and Trib3, encode pseudokinase proteins that have important roles in monocyte/macrophage proliferation, differentiation, and apoptosis. Trib1 is a critical factor that induces M2 macrophage differentiation in the bone marrow (BM). First, we investigated the proportion of M1 and M2 macrophages in BM mononuclear cells

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Lucas-Hahn, A., B. Petersen, M. Nowak-Imialek, et al. "122 A New Maturation Medium Improves Porcine Embryo Production In Vitro." Reproduction, Fertility and Development 30, no. 1 (2018): 200. http://dx.doi.org/10.1071/rdv30n1ab122.

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Recently (Spate et al. 2017 Reprod. Fertil. Dev. 29, 150), a new medium [TCM-199 supplemented with hCG 10 IU, pregnant mare serum gonadotropin (PMSG) 10 IU mL−1, fibroblast growth factor (FGF) 40 ng mL−1, leukemia inhibitory factor (LIF) 2000 U mL−1, IGF-1 20 ng mL−1, epidermal growth factor (EGF) 10 ng mL−1], termed FLI medium, was demonstrated to improve porcine oocyte maturation in vitro. The effects on embryo development and quality have not yet been investigated. The purpose of the present study was to compare the FLI medium in porcine in vitro embryo production (IVP) with our standard ma

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Korban, Schuyler S., Wannasiri Wannarat, Charlotte M. Rayburn, Tatiana C. Tatum, and A. Lane Rayburn. "Genome size and nucleotypic variation in Malus germplasm." Genome 52, no. 2 (2009): 148–55. http://dx.doi.org/10.1139/g08-109.

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The genus Malus has anywhere between 25 and 33 species along with several subspecies. Malus species as well as clones within the same species have varying ploidy levels, as these are more than likely collected from different trees and (or) from different locations. In recent years, large numbers of Malus germplasm accessions have been collected and maintained at the United States National Germplasm Clonal Repository; however, genome sizes of this material have not yet been determined. In this study, leaf tissues from young grafted trees of 100 Malus species and hybrids growing in a nursery at

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Bettelli, Alice, Rita Ruggiano, Silvia Bocchi, et al. "Inter-Cell Networking Profiling Enables Comprehensive Characterization of Immune-Mediated Activity of Anti-CD38 Therapy through Ex-Vivo Analysis of Multiple Myeloma Patients." Blood 134, Supplement_1 (2019): 3372. http://dx.doi.org/10.1182/blood-2019-126894.

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There has been a fast progress in clinical use of antibody-based immunotherapy, given the superior efficacy commonly achieved in clinic and the limited toxicity. However, personalization of treatment remains of major importance, both to achieve better clinical performance for monotherapy and to identify the best combinations on a patient-by-patient basis. Predicting patient's response is complex due to the need to characterize both tumor response and immunologic mechanisms possibly activated by the therapy, including antibody dependent cellular cytotoxicity (ADCC). We present the Inter-Cell Ne

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