Bibliografie tematiche / 16S rDNA

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Letteratura scientifica selezionata sul tema "16S rDNA"

Autore: Grafiati
Pubblicato: 4 giugno 2021
Ultima modifica: 25 luglio 2025

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Articoli di riviste sul tema "16S rDNA"

1

Fessehaie, A., S. H. De Boer, and C. A. Lévesque. "Molecular characterization of DNA encoding 16S–23S rRNA intergenic spacer regions and 16S rRNA of pectolyticErwiniaspecies." Canadian Journal of Microbiology 48, no. 5 (2002): 387–98. http://dx.doi.org/10.1139/w02-026.

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Sequences of 16S rDNAs and the intergenic spacer (IGS) regions between the 16S and 23S rDNA of bacterial strains from genus Erwinia were determined. Comparison of 16S rDNA sequences from different species and subspecies clearly revealed intraspecies–subspecies homology and interspecies heterogeneity. Phylogenetic analyses of 16S rDNA sequence data revealed that Erwinia spp. formed a discrete monophyletic clade with moderate to high bootstrap values. PCR amplification of the 16S–23S rDNA regions using primers complementary to the 3' end of 16S and 5' end of 23S rRNA genes generated two DNA frag
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Fukatsu, Takema, and Naruo Nikoh. "Two Intracellular Symbiotic Bacteria from the Mulberry Psyllid Anomoneura mori (Insecta, Homoptera)." Applied and Environmental Microbiology 64, no. 10 (1998): 3599–606. http://dx.doi.org/10.1128/aem.64.10.3599-3606.1998.

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ABSTRACT We characterized the intracellular symbiotic bacteria of the mulberry psyllid Anomoneura mori by performing a molecular phylogenetic analysis combined with in situ hybridization. In its abdomen, the psyllid has a large, yellow, bilobed mycetome (or bacteriome) which consists of many round uninucleated mycetocytes (or bacteriocytes) enclosing syncytial tissue. The mycetocytes and syncytium harbor specific intracellular bacteria, the X-symbionts and Y-symbionts, respectively. Almost the entire length of the bacterial 16S ribosomal DNA (rDNA) was amplified and cloned from the whole DNA o
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Wang, Haiyin, Pengcheng Du, Juan Li, et al. "Comparative analysis of microbiome between accurately identified 16S rDNA and quantified bacteria in simulated samples." Journal of Medical Microbiology 63, no. 3 (2014): 433–40. http://dx.doi.org/10.1099/jmm.0.060616-0.

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Although 16S rRNA gene (rDNA) sequencing is the gold standard for categorizing bacteria or characterizing microbial communities its clinical utility is limited by bias in metagenomic studies, in either the experiments or the data analyses. To evaluate the efficiency of current metagenomic methods, we sequenced seven simulated samples of ten bacterial species mixed at different concentrations. The V3 region of 16S rDNA was targeted and used to determine the distribution of bacterial species. The number of target sequences in individual simulated samples was in the range 1–1000 to provide a bett
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Riley, Donald E., Richard E. Berger, David C. Miner, and John N. Krieger. "Diverse and Related 16S rRNA-Encoding DNA Sequences in Prostate Tissues of Men with Chronic Prostatitis." Journal of Clinical Microbiology 36, no. 6 (1998): 1646–52. http://dx.doi.org/10.1128/jcm.36.6.1646-1652.1998.

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Treatment of chronic prostatitis/chronic pelvic pain syndrome is often empirical because clinical culture methods fail to detect prostate-associated pathogens in >90% of patients. Previously, we tested a variety of specific-microorganism PCRs and began a DNA sequence study after we found that 77% of prostatitis patients were PCR positive for prokaryotic rRNA-encoding DNA sequences (rDNAs) despite negative cultures using optimal techniques. In the present study, 36 rDNA clones from 23 rDNA-positive patients were sequenced. This study represents more than twice the total rDNA sequence and mor
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Dahllöf, Ingela, Harriet Baillie, and Staffan Kjelleberg. "rpoB-Based Microbial Community Analysis Avoids Limitations Inherent in 16S rRNA Gene Intraspecies Heterogeneity." Applied and Environmental Microbiology 66, no. 8 (2000): 3376–80. http://dx.doi.org/10.1128/aem.66.8.3376-3380.2000.

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ABSTRACT Contemporary microbial community analysis frequently involves PCR-amplified sequences of the 16S rRNA gene (rDNA). However, this technology carries the inherent problem of heterogeneity between copies of the 16S rDNA in many species. As an alternative to 16S rDNA sequences in community analysis, we employed the gene for the RNA polymerase beta subunit (rpoB), which appears to exist in one copy only in bacteria. In the present study, the frequency of 16S rDNA heterogeneity in bacteria isolated from the marine environment was assessed using bacterial isolates from the red alga Delisea p
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Reischl, U., K. Feldmann, L. Naumann, et al. "16S rRNA Sequence Diversity in Mycobacterium celatum Strains Caused by Presence of Two Different Copies of 16S rRNA Gene." Journal of Clinical Microbiology 36, no. 6 (1998): 1761–64. http://dx.doi.org/10.1128/jcm.36.6.1761-1764.1998.

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Direct sequencing of the 16S rRNA gene (16S rDNA) ofMycobacterium celatum isolates showed ambiguities, suggesting heterogeneity. Cloned 16S rDNA yielded two copies of the gene, which differed by insertion of a thymine at position 214 and by additional mismatches. Restriction fragment length polymorphism analysis confirmed the presence of two copies of 16S rDNA within the bacterial chromosome.
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BAO, Qiongli, Long-Jun DING, Yizong HUANG, and Keqing XIAO. "Effect of rice straw and/or nitrogen fertiliser inputs on methanogenic archaeal and denitrifying communities in a typical rice paddy soil." Earth and Environmental Science Transactions of the Royal Society of Edinburgh 109, no. 3-4 (2018): 375–86. http://dx.doi.org/10.1017/s1755691018000580.

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ABSTRACTTo understand better the microbial functional populations which are involved in methanogenesis and denitrification in paddy soils with rice straw (RS) and/or nitrogen fertiliser (potassium nitrate, N) application, the dynamics of methanogens and the denitrifying community were monitored simultaneously during the incubation period. The results show that the community structure of methanogens remained relatively stable among treatments based on 16S rDNA analysis, but fluctuated based on 16S rRNA. The Methanocellaceae and Methanosarcinaceae dominated all treatments at 16S rDNA and 16S rRN
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Lamy, Brigitte, Fréderic Laurent, and Angeli Kodjo. "Validation of a partialrpoBgene sequence as a tool for phylogenetic identification of aeromonads isolated from environmental sources." Canadian Journal of Microbiology 56, no. 3 (2010): 217–28. http://dx.doi.org/10.1139/w10-006.

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A collection of 50 aeromonads isolated from environmental sources were studied, together with all known Aeromonas nomenspecies, by phenotypic, amplified 16S rDNA restriction analysis (16S rDNA RFLP) and by partial sequence alignment of both 16S rDNA and rpoB genes. Although most of the type strain showed a unique phenotypic pattern, a database constructed on type strain phenotype allowed the identification of only 24% of the isolates. Analysis of 16S rDNA RFLP and the rpoB sequence were almost concordant in identifying environmental isolates at the species level, except for strains belonging t
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Hashavya, S., I. Gross, A. Michael-Gayego, N. Simanovsky, and R. Lamdan. "The efficacy of 16S ribosomal DNA sequencing in the diagnosis of bacteria from blood, bone and synovial fluid samples of children with musculoskeletal infections." Journal of Children's Orthopaedics 12, no. 2 (2018): 204–8. http://dx.doi.org/10.1302/1863-2548.12.170049.

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Background Musculoskeletal infections are among the most common bacterial infections in children leading to hospitalization, invasive procedures and prolonged antibiotic administration. Blood, synovial and sometimes tissue cultures are essential for the diagnosis and treatment of musculoskeletal infections; 16S ribosomal DNA (rDNA) sequencing is a novel diagnostic tool for the detection of bacteria. While the yield of 16S rDNA sequencing in synovial fluid was previously assessed, data regarding the efficacy of this method from blood samples or partially treated children with suspected musculos
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Bauernfeind, Adolf, Ines Schneider, Renate Jungwirth, and Carsten Roller. "Discrimination of Burkholderia multivorans and Burkholderia vietnamiensis fromBurkholderia cepacia Genomovars I, III, and IV by PCR." Journal of Clinical Microbiology 37, no. 5 (1999): 1335–39. http://dx.doi.org/10.1128/jcm.37.5.1335-1339.1999.

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We present a PCR procedure for identification of Burkholderia cepacia, Burkholderia multivorans, andBurkholderia vietnamiensis. 16S and 23S ribosomal DNAs (rDNAs) of B. multivorans and B. vietnamiensiswere sequenced and aligned with published sequences for definition of species-specific 18-mer oligonucleotide primers. Specific antisense 16S rDNA primers (for B. cepacia, 5′-AGC ACT CCC RCC TCT CAG-3′; for B. multivorans, 5′-AGC ACT CCC GAA TCT CTT-3′) and 23S rDNA primers (for B. vietnamiensis, 5′-TCC TAC CAT GCG TGC AA-3′) were paired with a general sense primer of 16S rDNAs (5′-AGR GTT YGA TY
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Tesi sul tema "16S rDNA"

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Adams, John D. W. "PCR amplification of Azospirillum 16S rDNA from natural environments." Thesis, University of Newcastle Upon Tyne, 1998. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.297528.

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Cheon, Ji Hoon. "Characterization and monitoring of microbial diversity in anaerobic bioreactor based on 16S rDNA." 京都大学 (Kyoto University), 2006. http://hdl.handle.net/2433/143997.

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Kyoto University (京都大学)<br>0048<br>新制・課程博士<br>博士(工学)<br>甲第12307号<br>工博第2636号<br>新制||工||1372(附属図書館)<br>24143<br>UT51-2006-J299<br>京都大学大学院工学研究科都市環境工学専攻<br>(主査)教授 津野 洋, 教授 武田 信生, 教授 田中 宏明<br>学位規則第4条第1項該当
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Toledo, Bethânia Figueiredo Barbosa de [UNESP]. "Identificação de estirpes de rizóbios por seqüenciamento parcial dos genes 16S rDNA e nifH." Universidade Estadual Paulista (UNESP), 2008. http://hdl.handle.net/11449/92670.

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Made available in DSpace on 2014-06-11T19:26:08Z (GMT). No. of bitstreams: 0 Previous issue date: 2008-10-29Bitstream added on 2014-06-13T18:54:07Z : No. of bitstreams: 1 toledo_bfb_me_jabo.pdf: 494396 bytes, checksum: 73007bbe5afed3d92412924b6ebe8b81 (MD5)<br>Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)<br>A crescente demanda de alimentos causada pelo aumento populacional, aliado a alto custo de fertilizantes industrializados e impacto ambiental causado por eles leva, mundialmente, à utilização em grande escala de inoculantes de bactérias fixadoras de nitrogênio. Os i
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Netto, Osmar Vaz de Carvalho. "Identificação de bactérias contaminantes de fermento de cachaça por seqüenciamento do gene 16S rDNA." Universidade de São Paulo, 2007. http://www.teses.usp.br/teses/disponiveis/11/11138/tde-08082007-164157/.

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A cachaça é uma bebida típica brasileira produzida a partir da destilação do caldo de canade- açúcar fermentado principalmente por Saccharomyces cerevisiae. Grande parte da produção nacional é artesanal, e não há uma preocupação por parte dos produtores quanto ao controle microbiológico da fermentação. Este trabalho objetivou caracterizar a comunidade bacteriana contaminante do fermento utilizado na produção de cachaça. Foram coletadas quatro amostras em um alambique artesanal. A primeira (NA) foi coletada um ano anterior às outras três e utilizada como controle. As restantes foram coletadas a
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Toledo, Bethânia Figueiredo Barbosa de. "Identificação de estirpes de rizóbios por seqüenciamento parcial dos genes 16S rDNA e nifH /." Jaboticabal : [s.n.], 2008. http://hdl.handle.net/11449/92670.

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Orientadora: Eliana Gertrudes de Macedo Lemos<br>Banca: Maria José Valarini<br>Banca: Jaime Maia dos Santos<br>Resumo: A crescente demanda de alimentos causada pelo aumento populacional, aliado a alto custo de fertilizantes industrializados e impacto ambiental causado por eles leva, mundialmente, à utilização em grande escala de inoculantes de bactérias fixadoras de nitrogênio. Os inoculantes utilizados no Brasil (coleção SEMIA- IPAGRO) ainda não estão suficientemente caracterizados geneticamente. O objetivo deste trabalho foi avaliar e confrontar as seqüências parciais dos genes 16S rDNA e ni
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Ziegler, Katie. "Phylogenetic Analysis of a Group of Enteric Bacteria Based on 16S rDNA Gene Sequencing." Miami University Honors Theses / OhioLINK, 2004. http://rave.ohiolink.edu/etdc/view?acc_num=muhonors1111684418.

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Wood, Jacqueline. "Analysis of bacterial populations from the rumen by means of 16S rDNA directed oligonucleotides." Thesis, University of Aberdeen, 1997. http://digitool.abdn.ac.uk/R?func=search-advanced-go&find_code1=WSN&request1=AAIU100186.

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The overall aim of this work was to develop molecular based techniques that would allow the identification and quantification of Prevotella spp. in samples of rumen fluid. This was achieved by developing two methods, both based on PCR amplification of 16S rDNA extracted from rumen and faecal samples. The first method involved the amplification of microbial DNA with universal primers, followed by probing with either a Bacteroides Prevotella specific oligonucleotide (Bac/Pre) or a universal oligonucleotide. Comparison with control DNA extracted from pure cultures allowed the relative abundances
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ARAÚJO, Livia Caroline Alexandre de. "Caracterização molecular das linhagens de Zymomonas mobilis da coleção de micro-organismos UFPEDA." Universidade Federal de Pernambuco, 2014. https://repositorio.ufpe.br/handle/123456789/17179.

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Submitted by Fabio Sobreira Campos da Costa (fabio.sobreira@ufpe.br) on 2016-06-29T12:07:11Z No. of bitstreams: 2 license_rdf: 1232 bytes, checksum: 66e71c371cc565284e70f40736c94386 (MD5) Dissertação Livia Araujo.pdf: 1983609 bytes, checksum: cbba526737c10f6743b3fd015372721a (MD5)<br>Made available in DSpace on 2016-06-29T12:07:11Z (GMT). No. of bitstreams: 2 license_rdf: 1232 bytes, checksum: 66e71c371cc565284e70f40736c94386 (MD5) Dissertação Livia Araujo.pdf: 1983609 bytes, checksum: cbba526737c10f6743b3fd015372721a (MD5) Previous issue date: 2014-02-20<br>CAPEs<br>Zymomonas mobilis vem de
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Naamala, J., SK Jaiswal, and FD Dakora. "Microsymbiont diversity and phylogeny of native bradyrhizobia associated with soybean (Glycine max L. Merr.) nodulation in South African soils." Systematic and Applied Microbiology, 2016. http://encore.tut.ac.za/iii/cpro/DigitalItemViewPage.external?sp=1001977.

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Abstract The genetic diversity and identification of slow- and fast- growing soybean root nodule bacterial isolates from different agro-climatic regions in Mpumalanga, Limpopo and Gauteng Provinces of South Africa were evaluated. The 16S-rDNA-RFLP analysis of 100 rhizobial isolates and eight reference type strains placed the isolates into six major clusters, and revealed their site-dependent genomic diversity. Sequence analysis of single and concatenated housekeeping genes (atpD, glnII and gyrB), as well as the symbiotic gene nifH captured a considerably higher level of genetic diversity and i
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Saraiva, Pedro Miguel Pinheiro. "Bacterial diversity in groundwater samples from Estremenho Karst Massif (Portugal) revealed by 16S rDNA pyrosequency." Master's thesis, Universidade de Aveiro, 2016. http://hdl.handle.net/10773/18544.

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Mestrado em Biologia Molecular e Celular<br>Groundwater provides an important freshwater source, that is many times overexploited and suffer pressures from superficial pollution. Therefore, it is of major interest to go further in the understanding of these environments. The present study examined, for the first time, the composition and diversity of bacterial communities present in groundwater from the Estremenho kart massif (Central-Western Portugal), through culture-independent molecular approaches, DGGE and pyrosequencing based on 16 rDNA sequences). Results showed that this particular en
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Libri sul tema "16S rDNA"

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Feddersen, Alix. 16S rDNA- und gyrA-Sequenzen als Differenzierungsmarker in der klinischen Mikrobiologie. 2000.

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Kemmerling, Cordula. Diagnostische 16S rDNA Oligonukleotid-Sonden für die Identifizierung von Vertretern der Aktinomyzeten-Gattung Streptosporangium. 1993.

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Capitoli di libri sul tema "16S rDNA"

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Arnemann, J. "16S-rDNA-Sequenzierung." In Springer Reference Medizin. Springer Berlin Heidelberg, 2019. http://dx.doi.org/10.1007/978-3-662-48986-4_3636.

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Arnemann, J. "16S-rDNA-Sequenzierung." In Lexikon der Medizinischen Laboratoriumsdiagnostik. Springer Berlin Heidelberg, 2018. http://dx.doi.org/10.1007/978-3-662-49054-9_3636-1.

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Rainey, Frederick A., and Erko Stackebrandt. "rDNA Amplification: Application of 16S rDNA-Based Methods for Bacterial Identification." In Nonradioactive Analysis of Biomolecules. Springer Berlin Heidelberg, 2000. http://dx.doi.org/10.1007/978-3-642-57206-7_34.

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Sklarz, Menachem Y., Roey Angel, Osnat Gillor, and Ines M. Soares. "Amplified rDNA Restriction Analysis (ARDRA) for Identification and Phylogenetic Placement of 16S-rDNA Clones." In Handbook of Molecular Microbial Ecology I. John Wiley & Sons, Inc., 2011. http://dx.doi.org/10.1002/9781118010518.ch7.

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Stackebrandt, Erko, and Fred A. Rainey. "Partial and complete 16S rDNA sequences, their use in generation of 16S rDNA phylogenetic trees and their implications in molecular ecological studies." In Molecular Microbial Ecology Manual. Springer Netherlands, 1995. http://dx.doi.org/10.1007/978-94-011-0351-0_18.

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Vinuesa, Pablo, L. W. Jan Rademaker, Frans J. de Bruijn, and Dietrich Werner. "Characterization of Bradyrhizobium SPP. Strains by Rflp analysis of Amplified 16s rDNA and rDNA Intergenic Spacer Regions." In Highlights of Nitrogen Fixation Research. Springer US, 1999. http://dx.doi.org/10.1007/978-1-4615-4795-2_56.

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Huo, Da, Yang Luo, Yunsi Nie, Jing Sun, Yanan Wang, and Zhiyi Qiao. "The Distribution Characteristics of Microcystis novacekii Based on 16S rDNA Sequence." In Lecture Notes in Electrical Engineering. Springer Berlin Heidelberg, 2015. http://dx.doi.org/10.1007/978-3-662-46318-5_4.

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Johansson, Karl-Erik, Malin U. K. Heldtander, and Bertil Pettersson. "Characterization of Mycoplasmas by PCR and Sequence Analysis with Universal 16S rDNA Primers." In Mycoplasma Protocols. Humana Press, 1998. http://dx.doi.org/10.1385/0-89603-525-5:145.

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Sedghi, Lea M., Stefan J. Green, and Craig D. Byron. "Measuring Effects of Dietary Fiber on the with of 16S rDNA Prior to Amplicon Synthesis." In Methods in Molecular Biology. Springer US, 2021. http://dx.doi.org/10.1007/978-1-0716-1518-8_16.

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Chakraborty, Argha, Aiswarya Bhattacharjee, Shibasis Mukherjee, and Gautam Chatterjee. "16S rDNA PCR-RFLP Method for Rapid Identification of Major Food Spoilage Bacterium Bacillus cereus." In Methods and Protocols in Food Science. Springer US, 2025. https://doi.org/10.1007/978-1-0716-4382-2_23.

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Atti di convegni sul tema "16S rDNA"

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Le Borgne, S., J. Jan, J. M. Romero, and M. Amaya. "Impact of Molecular Biology Techniques on the Detection and Characterization of Microorganisms and Biofilms Involved in MIC." In CORROSION 2002. NACE International, 2002. https://doi.org/10.5006/c2002-02461.

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Abstract Recently, the application of molecular techniques has greatly improved our knowledge of microbial diversity and distribution in environmental samples. In this paper, we have reviewed the molecular biology techniques that can be used to study microorganisms and biofilms involved in the MIC phenomenon. These techniques include 16S ribosomal DNA (16S rDNA) gene based techniques. In particular, 16S rDNA sequencing, the construction of 16S rDNAs libraries, Fluorescence In Situ Hybridization (FISH), Denaturing Gradient Gel Electrophoresis (DGGE) and Random Amplification of Polymorphic DNA (
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Horn, Joanne, Celena Carrillo, and Victoria Dias. "Comparison of the Microbial Community Composition at Yucca Mountain and Laboratory Test Nuclear Repository Environments." In CORROSION 2003. NACE International, 2003. https://doi.org/10.5006/c2003-03556.

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Abstract The microbiological community structure within a proposed nuclear waste repository at Yucca Mountain (YM), NV was determined. Microbial growth from collected rock was detected using simulated ground water as a growth medium, with or without amendment of a carbon source. Grown isolates were identified by 16S ribosomal DNA (rDNA) sequence analysis. A more complete compositional analysis of the microbial community located at the proposed nuclear waste repository site was performed using environmental DNA isolation and subsequent identification of amplified 16S rDNA genes. Concurrently, a
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Le Borgne, S., J. Jan, J. L. Garcia Villalobos, M. Amaya, and J. M. Romero. "Initial Developments of a Database of Bacteria Involved in Microbiologically Influenced Corrosion." In CORROSION 2004. NACE International, 2004. https://doi.org/10.5006/c2004-04058.

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Abstract An initial database of bacteria involved in MIC is proposed. It is composed of bacteria detected by Molecular Biology techniques through the sequencing of their 16S rDNA genes in bio films, consortia and samples collected in sites potentially affected by MIC problems. The bacteria were not isolated and their participation in MIC should be evaluated. The use of such data is discussed and future directions are also proposed to continue this work.
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De Paula, Renato, Cruz St Peter, Ian Alex Richardson, et al. "DNA Sequencing of Oilfield Samples: Impact of Protocol Choices on the Microbiological Conclusions." In CORROSION 2018. NACE International, 2018. https://doi.org/10.5006/c2018-11662.

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Abstract In the last decade, molecular microbiology techniques have significantly expanded the understanding of the resident microflora in hydrocarbon reservoirs and production systems. These methods have been steadily accepted by the industry and are widely viewed as accurate, comprehensive and highly valuable tools that augment or may eventually replace conventional methods. The resulting information has helped operators and service companies to develop better monitoring programs, assess risks and tailor mitigation strategies to control undesired microbial activities in wells, flowlines and
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Bartling, Craig, Angela Minard-Smith, Kate H. Kucharzyk, Jennifer Busch-Harris, and Larry Mullins. "Interpreting Omic Data for Microbially Influenced Corrosion: Lessons from a Case Study Involving a Seawater Injection System." In CORROSION 2017. NACE International, 2017. https://doi.org/10.5006/c2017-09445.

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Abstract Microbiologically influenced corrosion (MIC) is a significant source of pitting corrosion affecting oil and gas pipelines, wells, and a variety of surface facilities. Understanding of MIC is greatly enhanced through DNA and protein sequencing technologies. This paper highlights the need to understand the methods used to generate the data, the data quality, and the limitations associated with data interpretation through a case study involving the metagenomics and proteomic analysis of pig envelope debris and seawater samples from various locations within a seawater injection system sus
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Wang, Linna, Claudia C. Pierce, Dorothy Reynolds, and Elizabeth Summer. "DNA Based Diversity Analysis of Microorganisms in Industrial Cooling Towers." In CORROSION 2017. NACE International, 2017. https://doi.org/10.5006/c2017-09483.

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Abstract Effective microbial control in cooling systems is necessary to ensure system cleanliness and avoid fouling that degrades cooling system performance, promotes corrosion and favors growth of pathogens. However, controlling organisms optimally involves an understanding of the identity of the population of microbes in a system due to the varying susceptibilities of organisms to biocides. This is a challenging task with standard culturing techniques which only allow for a small fraction of the total population to be cultured and identified. In this study, 16s rDNA was employed to maximize
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Romero, J. M., E. Velázquez, J. L. García Villalobos, M. Amaya, and S. Le Borgne. "Genetic Monitoring of Bacterial Populations in a Seawater Injection System. Identification of Biocide Resistant Bacteria and Study of Their Corrosive Effect." In CORROSION 2005. NACE International, 2005. https://doi.org/10.5006/c2005-05483.

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Abstract DNA was extracted from a water sample taken from an offshore seawater injection system. DNA was also extracted from enrichment cultures from the same sample. The V3 hypervariable region of the 16S rDNA gene was amplified by the Polymerase Chain Reaction (PCR) and bacterial diversity was studied using Denaturing Gel Gradient Electrophoresis (DGGE). The obtained results showed that microbial evaluation was biased by the use of artificial culture media although recommended media were used, indicating that microbiological analysis of waters in industrial systems by culturing methods may n
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8

Le Borgne, S., J. M. Romero, H. A. Videla, J. M. Gonzalez, and C. Saiz-Jiménez. "Practical Cases of the Use of Molecular Techniques to Characterize Microbial Deterioration of Metallic Structures in Industry." In CORROSION 2007. NACE International, 2007. https://doi.org/10.5006/c2007-07523.

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Abstract Two specific cases of applying molecular techniques for deciphering the role of microorganisms in industrial processes are presented: an offshore seawater injection system and a wastewater treatment plant. In the first case, deoxyribonucleic acid (DNA) was extracted from a water sample taken from an offshore seawater injection system and from enrichment cultures from the same sample. The V3 hypervariable region of the 16S rDNA gene was amplified by the polymerase chain reaction (PCR) and bacterial diversity was studied using denaturing gel gradient electrophoresis (DGGE). DGGE monitor
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Gu, Ji-Dong, and Ralph Mitchell. "Degradation of Polyurethane by a Bacterium Isolated from Soil." In CORROSION 2004. NACE International, 2004. https://doi.org/10.5006/c2004-04584.

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Abstract Polymeric materials are widely used in engineering facilities for protection against corrosion and growth of microorganisms. They are known to be susceptible to microbial degradation, but few investigations have examined both the basic microbiology and the biochemical mechanisms of degradation involved. An enrichment culture was established using water-soluble polyurethane as the sole source of carbon and energy and soil as an inoculum. In subsequent enrichment transfers in minimum salt medium supplemented with polyurethane, growth of the bacterial consortium was observed, indicating
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Norashirene, M. J., A. Ahmad Amin, D. Norhidayah, and M. A. Nurul Fithriah. "Identification of cellulolytic thermophiles based on 16S rDNA gene amplification analysis." In 2012 IEEE Colloquium on Humanities, Science and Engineering Research (CHUSER). IEEE, 2012. http://dx.doi.org/10.1109/chuser.2012.6504349.

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Rapporti di organizzazioni sul tema "16S rDNA"

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ทองเกิด, ปิโยรส. ความหลากชนิดและดีเอ็นเอบาร์โค้ดของสัตว์ไม่มีกระดูกสันหลังหน้าดินขนาดใหญ่ ในพื้นที่ศูนย์เครือข่ายการเรียนรู้เพื่อภูมิภาค จุฬาลงกรณ์มหาวิทยาลัย จังหวัดสระบุรี : รายงานผลการดำเนินงาน. จุฬาลงกรณ์มหาวิทยาลัย, 2017. https://doi.org/10.58837/chula.res.2017.39.

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จากผลการสำรวจตัวอย่างสัตว์ไม่มีกระดูกสันหลังหน้าดินขนาดใหญ่ ประกอบด้วย หอยทากบก ไส้เดือน กิ้งกือ และตะขาบ ในพื้นที่ศูนย์เครือข่ายการเรียนรู้เพื่อภูมิภาค จุฬาลงกรณ์มหาวิทยาลัย จังหวัดสระบุรี พบหอยทากบกทั้งหมด 3 วงศ์ 4 สปีชีส์ ไส้เดือน 2 สกุล 3 สปีชีส์ กิ้งกือ 5 อันดับ 7 สปีชีส์ และตะขาบ 2 อันดับ 2 สปีชีส์ ในการศึกษาปีแรกนี้ได้ทำการเก็บข้อมูลพื้นฐานทางนิเวศวิทยา การจำเพาะถิ่น การกระจาย และดีเอ็นเอบาร์โค้ดของสัตว์ไม่มีกระดูกสันหลังหน้าดินขนาดใหญ่ ได้แก่ หอยทากบกที่พบทุกสปีชีส์ ทั้งข้อมูลของยีนในไมโตคอนเดียร์ (16S rDNA และ COI) และยีนในนิวเคลียส (5.8S, 18S, 28S rDNA และ ITS2) เพื่อทำเป็นฐานข้อมูลด
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อัศวลาภสกุล, วันชัย, กนกพร ไตรวิทยากร, ศุภจิต สระเพชร, ณัฐพล อภิรติกุล та วัฒนชัย จำปาทอง. การแยกและศึกษาสมบัติของเชื้อเอนโดไฟท์ที่เจริญอยู่ในมันสำปะหลัง และการประยุกต์ใช้ : รายงานการวิจัย. คณะวิทยาศาสตร์ จุฬาลงกรณ์มหาวิทยาลัย, 2017. https://doi.org/10.58837/chula.res.2017.57.

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โครงการนี้เป็นโครงการปีที่ 2 ของการศึกษาหาเอนโดไฟท์ที่เจริญอยู่ในส่วนต่าง ๆ ของมันสำปะหลังที่ เจริญอยู่ร่วมกับมันสำปะหลังจำนวน 3 สายพันธุ์ ได้แก่ 1) พันธุ์ห้วยบง 60 2) พันธุ์เกษตรศาสตร์ 50 และ 3) พันธุ์พิรุณ 1 สามารถแยกเอนโดไฟท์ได้ 67, 60, และ 42 ไอโซเลทตามลำดับ จากนั้นนำประชากรเอนโดไฟท์ที่ แยกได้มาทดสอบสมบัติทางชีวภาพที่มีประโยชน์ในการดำรงชีวิตต่อพืช ได้แก่ ความสามารถในการสร้างกรด อินโดลอะซีติก ความสามารถในการเพิ่มการละลายฟอสเฟต และการสร้างสารไซเดอโรฟอร์ รวมถึงการศึกษา ลำดับนิวคลีโอไทด์ 16S rDNA พบว่า เอนโดไฟท์มีสมบัติในการสร้างกรดอินโดลอะซีติก สมบัติในการละลาย ฟอสเฟตในดิน และสมบัติในการสร้าง
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ทองเกิด, ปิโยรส. ความหลากชนิดและดีเอ็นเอบาร์โค้ดของสัตว์ไม่มีกระดูกสันหลังหน้าดินขนาดใหญ่ ในพื้นที่ศูนย์เครือข่ายการเรียนรู้เพื่อภูมิภาค จุฬาลงกรณ์มหาวิทยาลัย จังหวัดสระบุรี : รายงานผลการดำเนินงาน. คณะวิทยาศาสตร์ จุฬาลงกรณ์มหาวิทยาลัย, 2018. https://doi.org/10.58837/chula.res.2018.54.

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จากผลการสำรวจตัวอย่างสัตว์ไม่มีกระดูกสันหลังหน้าดินขนาดใหญ่ ประกอบด้วย หอยทากบก ไส้เดือน กิ้งกือ และตะขาบ ในพื้นที่ศูนย์เครือข่ายการเรียนรู้เพื่อภูมิภาค จุฬาลงกรณ์มหาวิทยาลัย จังหวัดสระบุรี พบหอยทากบกทั้งหมด 3 วงศ์ 4 สปีชีส์ ไส้เดือน 2 สกุล 3 สปีชีส์ กิ้งกือ 5 อันดับ 12 สปีชีส์ และตะขาบ 2 อันดับ 2 สปีชีส์ ในการศึกษาปีแรกนี้ได้ทำการเก็บข้อมูลพื้นฐานทางนิเวศวิทยา การจำเพาะถิ่น การกระจาย และดีเอ็นเอบาร์โค้ดของสัตว์ไม่มีกระดูกสันหลังหน้าดินขนาดใหญ่ ได้แก่ กิ้งกือที่พบทุกสปีชีส์ ทั้งข้อมูลของยีนในไมโตคอนเดียร์ (16S rDNA และ COI) เพื่อทำเป็นฐานข้อมูลดีเอ็นเอและใช้ประโยชน์ในเชิงอนุกรมวิธานและการศึกษา
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ปิยะธีรธิติวรกุล, สมเกียรติ, та ศิริรัตน์ เร่งพิพัฒน์. แลกติกแอซิดแบคทีเรียที่ใช้เป็นโพรไบโอติกสำหรับปลากะพงขาว Lates calcarifer : รายงานฉบับสมบูรณ์. ศูนย์เชี่ยวชาญเฉพาะทางด้านเทคโนโลยีชีวภาพทางทะเล ภาควิชาวิทยาศาสตร์ทางทะเล คณะวิทยาศาสตร์ จุฬาลงกรณ์มหาวิทยาลัย, 2005. https://doi.org/10.58837/chula.res.2005.76.

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การแยกแลกติกแอซิดแบคทีเรียจากทางเดินอาหารของปลากะพงขาว เพื่อคัดเลือกแบคทีเรียที่มีสมบัติเป็นโพรไบโอติก ด้วยเทคนิค well agar diffusion ผลพบว่า มี 5 ไอโซเลต (กำหนดให้เป็น LAB-1 - LAB-5) ที่สามารถยับยั้งการเจริญของ Aeromonas hydrophila แบคทีเรียก่อโรคในปลาได้ ผลการวิเคราะห์ปริมาณองค์ประกอบในอาหารผสมแบบเปียก พบว่า ปริมาณสารอาหารต่างๆ ในอาหารมีเพียงพอกับความต้องการของปลากะพงขาว ยกเว้นปริมาณไขมันที่ต่ำเกินไป ความเข้มข้นของ A. hydrophila ที่ทำให้ปลาตาย 50% (LC50) หลังจากที่ปลาได้รับเชื้อแล้ว 72 ชั่วโมง มีค่าเท่ากับ 7.76 log10 เซลล์/มล. และที่ 96 และ 120 ชั่วโมง มีค่าเท่ากับ 7.47 และ 7.26 log10 เซลล์/
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ธนียวัน, จิราภรณ์, กอบชัย ภัทรกุลวณิชย์ та ขนิษฐา วงษ์นิกร. การโคลนและลักษณะสมบัติของยีนกลุ่ม rhl ที่เกี่ยวข้องกับการสังเคราะห์โมโนแรมโนลิพิดทางชีวภาพของ Pseudomonas aeruginosa A41 : รายงานผลการวิจัย. จุฬาลงกรณ์มหาวิทยาลัย, 2006. https://doi.org/10.58837/chula.res.2006.41.

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Pseudomonas sp. A41 ที่แยกจากน้ำทะเลอ่าวไทย มีความสามารถในการผลิตแรมโนลิพิดจากลักษณะทางสัณฐานวิทยาและสมบัติทางชีวเคมีร่วมกับข้อมูลลำดับนิวคลีโอไทด์ของ 16S rDNA สามารถจำแนกสายพันธุ์ A41 เป็น Pseudomonas aeruginosa แยกยีนที่เกี่ยวข้องกับการสังเคราะห์แรมโนลิพิดจาก Pseudomonas aeruginosa A41 โดยเทคนิคเซาท์เธอร์นไฮบริไดเซชันจีโนมิกดีเอ็นเอกับดีเอ็นเอติดตาม rhlR หรือ rhlA โคลนชิ้นดีเอ็นเอที่ให้ผลบวกเข้าในพลาสมิดเวกเตอร์และหาลำดับนิวคลีโอไทด์ของชิ้นดีเอ็นเอแทรกสอด จากลำดับนิวคลีโอไทด์ทั้งสิ้น 4,965 bp พบกรอบอ่านรหัสเปิด (ORF) ที่สมบูรณ์จำนวน 4 แห่ง และไม่สมบูรณ์ 1 แห่ง กรอบอ่านรหัสเปิดทั้งหมดมีทิศทาง
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ธนาศุภวัฒน์, สมบูรณ์. อนุกรมวิธานและเอนไซม์โปรทีเอสของแบคทีเรียชอบเค็มจากอาหารหมัก : รายงานผลการวิจัย. จุฬาลงกรณ์มหาวิทยาลัย, 2007. https://doi.org/10.58837/chula.res.2007.33.

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การศึกษาอนุกรมวิธานแบคทีเรียชอบเค็มที่สามารถผลิตเอนไซม์โปรติเอสจากอาหารหมักชนิดต่าง ๆ ที่เก็บจากตลาด และจากน้ำปลาที่ผลิตในจังหวัดสมุทรปราการ สมุทรสงคราม ระยอง รวมทั้งจากบูดูในจังหวัดปัตตานี รวม 110 ตัวอย่าง พบว่าสามารถแยกแบคทีเรียชอบเค็มได้จำนวน 279 ไอโซเลต จากผลการคัดเลือกแบคทีเรียชอบเค็มที่สามารถผลิตเอนไซม์โปรติเอสขั้นต้น พบแบคทีเรียชอบเค็มปานกลาง 38 ไอโซเลต และแบคทีเรียชอบเค็มสูง 7 ไอโซเลต จากการศึกษาทางอนุกรมวิธานของแบคทีเรียตัวแทน 82 สายพันธุ์ โดยอาศัยลักษณะทางฟีโนไทป์ ลักษณะทางอนุกรมวิธานเคมี และ DNA-DNA similarity รวมทั้งการวิเคราะห์ลำดับเบสในช่วง 16S rDNA สามารถพิสูจน์เอกลักษณ์ของแบคที
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Jurkevitch, Edouard, Carol Lauzon, Boaz Yuval, and Susan MacCombs. role of nitrogen-fixing bacteria in survival and reproductive success of Ceratitis capitata, the Mediterranean fruit fly. United States Department of Agriculture, 2005. http://dx.doi.org/10.32747/2005.7695863.bard.

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Objectives: to demonstrate nitrogen fixation in the gut of Ceratitiscapitata, the Mediterranean fruit fly and that fixed nitrogen is important for the fly. Background: Fruit flies (Diptera: Tephritidae) are a highly successful, widespread group of insects causing enormous economic damage in agriculture. They are anautogenous, i.e. the acquisition of nitrogenous compounds by both male and female is essential for the realization of their reproductive potential. Nitrogen, although abundant in the atmosphere, is paradoxically a limiting resource for multicellular organisms. In the Animalia, biolog
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Bercovier, Herve, and Paul Frelier. Pathogenic Streptococcus in Tilapia: Rapid Diagnosis, Epidemiology and Pathophysiology. United States Department of Agriculture, 1994. http://dx.doi.org/10.32747/1994.7568776.bard.

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Within the project "Pathogenic Streptococcus in Tilapia", gram positive cocci pathogens of fish in Israel and in the United States were characterized. We showed that Streptococcus shiloi, the name for an agent causing septicemic infection in fish, is a junior synonym of Streptococcus iniae and that Enterococcus seriolicida is a junior synonym of Lactococcus garvieae, a causative agent of septicemia and meningo-encephalitis in fish. Molecular epidemiology studies on these two pathogens, based on 16S rDNA sequences and ribotyping showed that although each country had specific clones, S. iniae or
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Gallego Sánchez, Gerardo J., Patricia Zapata, Oscar Castañeda, et al. Use of DNA sequences for identification of possible biotypes of the fruit borer Neoleucinodes elegantalis (Lepidoptera: Crambidae), an important pest of Andean solanaceous fruits. Centro Internacional de Agricultura Tropical (CIAT), 2015. http://dx.doi.org/10.21930/agrosavia.poster.2015.1.

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In Colombia, Venezuela, Ecuador, Brazil and Honduras, the tomato borer, Neoleucinodes elegantalis, is the most important fruit-related plague of the Solanaceae family. A suitable molecular characterization using a DNA barcoding system is necessary to clarify different issues inside the taxonomy of Neoleucinodes genus. Additionally, other DNA sequences used for molecular identification and phylogenetics studies, can be implemented to obtain a better understanding of the genetic variability across different animal groups and allows to acquire a enhanced description of the population s genetic va
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