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Articoli di riviste sul tema "Allergen microarrays"

Autore: Grafiati

Pubblicato: 21 settembre 2024

Ultima modifica: 31 luglio 2025

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1

Sokolov, Pavel, Irina Evsegneeva, Alexander Karaulov, Alyona Sukhanova, and Igor Nabiev. "Allergen Microarrays and New Physical Approaches to More Sensitive and Specific Detection of Allergen-Specific Antibodies." Biosensors 14, no. 7 (2024): 353. http://dx.doi.org/10.3390/bios14070353.

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The prevalence of allergic diseases has increased tremendously in recent decades, which can be attributed to growing exposure to environmental triggers, changes in dietary habits, comorbidity, and the increased use of medications. In this context, the multiplexed diagnosis of sensitization to various allergens and the monitoring of the effectiveness of treatments for allergic diseases become particularly urgent issues. The detection of allergen-specific antibodies, in particular, sIgE and sIgG, is a modern alternative to skin tests due to the safety and efficiency of this method. The use of al

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Gipson, Stephen A. Y., Jacqueline B. Nesbit, Lauren T. Swientoniewski, et al. "Purification and Epitope Mapping of Jug r 4, a Major Walnut Allergen." Allergies 5, no. 1 (2025): 8. https://doi.org/10.3390/allergies5010008.

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Background: Tree nut allergy affects approximately 1% of the U.S. population and the prevalence is increasing. Walnut allergy is the most commonly reported tree nut allergy in the United States. This study aimed to investigate the IgE cross-reactivity between walnut allergen Jug r 4 and peanut allergen Ara h 3 in individuals with dual walnut and peanut allergies. Methods: Jug r 4 was purified from whole walnut extract and analyzed via western blot using anti-Ara h 3 antibodies alongside serum IgE from walnut allergic patients. Sera from individuals allergic to both peanuts and walnuts were uti

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Klimek, L., D. Vetter, L. von Bernus, and C. Thorn. "Allergen-Microarrays für die molekulare Komponentendiagnostik von Typ-I-Allergien." HNO 59, no. 10 (2010): 988–93. http://dx.doi.org/10.1007/s00106-010-2224-5.

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Romantowski, Jan, Aleksandra Górska, Grażyna Moszkowska, et al. "Atopy and Multisensitizations in Specific IgE Microarrays and Their Impact on Severe Asthma." Life 12, no. 10 (2022): 1520. http://dx.doi.org/10.3390/life12101520.

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(1) Asthma is a chronic inflammatory airway disease. Around 3–10% of patients experience severe refractory asthma. These patients with high symptom intensity and frequent exacerbations present a challenge for allergologists. Their allergic vs. non-allergic profile might be different from the standard asthmatic group and this difference is vital in qualifying for anti-IgE biologicals. The aim of the study was to analyze multiple sensitizations in patients with severe asthma and assess their impact on the course of the disease. (2) Forty-two patients with severe asthma according to GINA were enr

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Cretich, Marina, Daniela Breda, Francesco Damin, et al. "Allergen microarrays on high-sensitivity silicon slides." Analytical and Bioanalytical Chemistry 398, no. 4 (2010): 1723–33. http://dx.doi.org/10.1007/s00216-010-4077-x.

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Jeon, Hyunjin, Joo Hyun Jung, Yoonji Kim, B.S., Youngeun Kwon, and Seon Tae Kim. "Allergen Microarrays forIn VitroDiagnostics of Allergies: Comparison with ImmunoCAP and AdvanSure." Annals of Laboratory Medicine 38, no. 4 (2018): 338–47. http://dx.doi.org/10.3343/alm.2018.38.4.338.

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Szameit, Sandra, Elisabeth Weber, and Christa Noehammer. "DNA microarrays provide new options for allergen testing." Expert Review of Molecular Diagnostics 9, no. 8 (2009): 843–50. http://dx.doi.org/10.1586/erm.09.63.

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Baumgart, Karl. "The whether, whither or wither of allergen microarrays." Pathology 56 (February 2024): S31. http://dx.doi.org/10.1016/j.pathol.2023.12.114.

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Romantowski, Jan, Aleksandra Górska, Marek Niedoszytko, et al. "A Challenge for Allergologist: Application of Allergy Diagnostic Methods in Mast Cell Disorders." International Journal of Molecular Sciences 22, no. 3 (2021): 1454. http://dx.doi.org/10.3390/ijms22031454.

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Primary and secondary mast cell activation syndromes (MCAS) can occur in patients with mastocytosis. During the past few years our knowledge about the pathogenesis and disease-triggering mechanisms in MCAS and mastocytosis have increased substantially. Whereas mastocytosis is characterized by an accumulation of neoplastic (clonal) mast cells (MC) in various organ systems, MCAS is defined by a massive and systemic activation of these cells. Mast cells are crucial effector cells in allergic diseases, thus their elevated number and activation can cause severe anaphylactic reactions and MCAS in pa

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Chinnasamy, Thiruppathiraja, Loes I. Segerink, Mats Nystrand, Jesper Gantelius, and Helene Andersson Svahn. "Point-of-Care Vertical Flow Allergen Microarray Assay: Proof of Concept." Clinical Chemistry 60, no. 9 (2014): 1209–16. http://dx.doi.org/10.1373/clinchem.2014.223230.

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Abstract BACKGROUND Sophisticated equipment, lengthy protocols, and skilled operators are required to perform protein microarray-based affinity assays. Consequently, novel tools are needed to bring biomarkers and biomarker panels into clinical use in different settings. Here, we describe a novel paper-based vertical flow microarray (VFM) system with a multiplexing capacity of at least 1480 microspot binding sites, colorimetric readout, high sensitivity, and assay time of &lt;10 min before imaging and data analysis. METHOD Affinity binders were deposited on nitrocellulose membranes by conve

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Cretich, Marina, Gabriele Di Carlo, Cinzia Giudici, et al. "Detection of allergen specific immunoglobulins by microarrays coupled to microfluidics." PROTEOMICS 9, no. 8 (2009): 2098–107. http://dx.doi.org/10.1002/pmic.200800651.

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Fall, Barbara I., Bernadette Eberlein-König, Heidrun Behrendt, Reinhard Niessner, Johannes Ring, and Michael G. Weller. "Microarrays for the Screening of Allergen-Specific IgE in Human Serum." Analytical Chemistry 75, no. 3 (2003): 556–62. http://dx.doi.org/10.1021/ac026016k.

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Foncy, Julie, Erwan Crestel, Jean-Philippe Borges, et al. "Reversible magnetic clamp of a microfluidic interface for the seric detection of food allergies on allergen microarrays." Microelectronic Engineering 158 (June 2016): 16–21. http://dx.doi.org/10.1016/j.mee.2016.03.005.

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Tuppo, Lisa, Ivana Giangrieco, Maurizio Tamburrini, Claudia Alessandri, Adriano Mari, and Maria Antonietta Ciardiello. "Detection of Allergenic Proteins in Foodstuffs: Advantages of the Innovative Multiplex Allergen Microarray-Based Immunoassay Compared to Conventional Methods." Foods 11, no. 6 (2022): 878. http://dx.doi.org/10.3390/foods11060878.

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Several factors can affect the allergen content and profile of a specific food, including processing procedures often leading to a decrease in allergenicity, although no change, or even an increase, have also been reported. Evaluation of the effectiveness of a processing procedure requires the availability of reliable methodologies to assess the variation in molecules able to induce allergic reactions in the analyzed food. Conventional and innovative strategies and methodologies can be exploited to identify allergenic proteins in foodstuffs. However, depending on the specific purposes, differe

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Bacarese-Hamilton, Tito, Letizia Mezzasoma, Colin Ingham, et al. "Detection of Allergen-specific IgE on Microarrays by Use of Signal Amplification Techniques." Clinical Chemistry 48, no. 8 (2002): 1367–70. http://dx.doi.org/10.1093/clinchem/48.8.1367.

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Canonica, G. Walter, Giovanni Passalacqua, and Gianni Melioli. "Poster 1001: ALLERGENIUS®: an expert system for the interpretation of allergen microarrays." World Allergy Organization Journal 7 (2014): P2. http://dx.doi.org/10.1186/1939-4551-7-s1-p2.

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Prosperi, Mattia C. F., Danielle Belgrave, Iain Buchan, Angela Simpson, and Adnan Custovic. "Challenges in interpreting allergen microarrays in relation to clinical symptoms: A machine learning approach." Pediatric Allergy and Immunology 25, no. 1 (2013): 71–79. http://dx.doi.org/10.1111/pai.12139.

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Wiltshire, Steve, Shawn O’Malley, Jeremy Lambert, et al. "Detection of Multiple Allergen-specific IgEs on Microarrays by Immunoassay with Rolling Circle Amplification." Clinical Chemistry 46, no. 12 (2000): 1990–93. http://dx.doi.org/10.1093/clinchem/46.12.1990.

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Harris, Jennifer, Daniel E. Mason, Jun Li, et al. "Activity Profile of Dust Mite Allergen Extract Using Substrate Libraries and Functional Proteomic Microarrays." Chemistry & Biology 11, no. 10 (2004): 1361–72. http://dx.doi.org/10.1016/j.chembiol.2004.08.008.

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Ott, Hagen, Regina Fölster-Holst, Hans F. Merk, and Jens Malte Baron. "Allergen microarrays: a novel tool for high-resolution IgE profiling in adults with atopic dermatitis." European Journal of Dermatology 20, no. 1 (2010): 054–61. http://dx.doi.org/10.1684/ejd.2010.0810.

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Garriga-Baraut, Teresa, Moises Labrador-Horrillo, Mercé Tena, et al. "A real-life ImmunoCAT study: impact of molecular diagnosis through ImmunoCAPTM ISAC 112 on immunotherapy prescription in pollen-polysensitized patients in Catalonia, Spain." Allergologia et Immunopathologia 52, no. 4 (2024): 21–29. http://dx.doi.org/10.15586/aei.v52i4.1077.

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Background: Molecular diagnosis in allergology helps to identify multiple allergenic molecules simultaneously. The use of purified and/or recombinant allergens increases the accuracy of individual sensitization profiles in allergic patients. Objective: To assess the impact of molecular diagnosis through the ImmunoCAPTM ISAC 112 microarray on etiological diagnosis and specific immunotherapy (SIT) prescription. This was compared to the use of conventional diagnoses in pediatric, adolescent, and young adult patients with rhinitis or rhinoconjunctivitis and/or allergic asthma, sensitized to three

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Mullenix, Michael C., Steve Wiltshire, Weiping Shao, Gary Kitos, and Barry Schweitzer. "Allergen-specific IgE Detection on Microarrays Using Rolling Circle Amplification: Correlation with in Vitro Assays for Serum IgE." Clinical Chemistry 47, no. 10 (2001): 1929. http://dx.doi.org/10.1093/clinchem/47.10.1926.

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23

Shahali, Y., P. Nicaise, A. Brázdová, et al. "Complementarity between Microarray and Immunoblot for the Comparative Evaluation of IgE Repertoire of French and Italian Cypress Pollen Allergic Patients." Folia Biologica 60, no. 4 (2014): 192–201. http://dx.doi.org/10.14712/fb2014060040192.

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Cypress pollen represents the primary cause of respiratory allergies in Mediterranean areas. Patients allergic to Cupressus sempervirens pollen (Cups) (CPA) can be discriminated on the basis of the immunoglobulin E (IgE) binding to a basic 14 kDa protein (BP14) or to high-molecular-weight (HMW) glycoproteins only. Specific IgE repertoires of two differentially exposed CPA cohorts, French and Italian, were investigated using an IgE microarray system (some known major allergens from several allergenic sources) and individual IgE immunoblotting (IB) of whole Cups pollen extract separated by SDS-P

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24

Loffredo, Lucas, and Sergejs Berdnikovs. "Microarray analysis of epithelial-to-mesenchymal transition genes suggests differential epithelial remodeling in human disease vs. mouse models of asthma (MUC1P.912)." Journal of Immunology 194, no. 1_Supplement (2015): 64.13. http://dx.doi.org/10.4049/jimmunol.194.supp.64.13.

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Abstract Epithelial-mesenchymal transition (EMT) is the process by which epithelial cells revert to a less differentiated, mesenchymal phenotype; it occurs in maintenance of tissue homeostasis and is exaggerated in disease. EMT has been implicated in human asthma, although it is not clear whether this process is caused by inflammation or rather contributes to it. In allergen-induced murine models of asthma, EMT would be directly caused by inflammation; however, evidence for EMT in such models is lacking. To assess weight-of-evidence and causality for EMT, we contrasted gene expression between

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Miralles-Lopez, Juan Carlos, Antonio Carbonell-Martínez, Soledad Zamarro-Parra, et al. "Clinical and serological characteristics of patients allergic to LTP." Allergologia et Immunopathologia 52, no. 4 (2024): 9–14. http://dx.doi.org/10.15586/aei.v52i4.1074.

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Background: Allergy to lipid transfer proteins (LPT) is common in Mediterranean Europe, and it causes severe reactions in patients and affects multiple foods, impairing the quality of life. Objective: This study aimed to describe the clinical and sensitization profile of patients with LTP syndrome and to determine a clinical pattern of severity. Molecular diagnosis is shown in a broad population through microarrays. Material and Methods: This study was performed at the LTP Allergy Consultation of the Reina Sofia Hospital in Murcia, Spain. We analyzed the patients’ characteristics, reactions, c

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Mathae, Laura, Itziar Martinez-Gonzalez, and Fumio Takei. "Gene expression profile of allergen-experienced group 2 innate lymphoid cells (HYP2P.323)." Journal of Immunology 194, no. 1_Supplement (2015): 53.4. http://dx.doi.org/10.4049/jimmunol.194.supp.53.4.

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Abstract We previously identified group 2 innate lymphoid cells (ILC2s) in mouse lungs. Upon allergen encounter, they produce type 2 cytokines and mediate T cell-independent allergic lung inflammation and also promote Th2 responses. We have recently found that allergen-treatments of mice result in significant increase in the number of ILC2s in the lung and lymph node even after the resolution of inflammation for several weeks. The allergen-experienced ILC2s are more responsive to unrelated allergens than naive ILC2s and rapidly induce allergic lung inflammation. To characterize the allergen-ex

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Niespodziana, Katarzyna, Katarina Stenberg-Hammar, Nikolaos G. Papadopoulos, et al. "Microarray Technology May Reveal the Contribution of Allergen Exposure and Rhinovirus Infections as Possible Triggers for Acute Wheezing Attacks in Preschool Children." Viruses 13, no. 5 (2021): 915. http://dx.doi.org/10.3390/v13050915.

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Allergen exposure and rhinovirus (RV) infections are common triggers of acute wheezing exacerbations in early childhood. The identification of such trigger factors is difficult but may have therapeutic implications. Increases of IgE and IgG in sera, were shown against allergens and the N-terminal portion of the VP1 proteins of RV species, respectively, several weeks after allergen exposure or RV infection. Hence, increases in VP1-specific IgG and in allergen-specific IgE may serve as biomarkers for RV infections or allergen exposure. The MeDALL-allergen chip containing comprehensive panels of

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Figo, Daniele Danella, Priscilla Rios Cordeiro Macedo, Gabriele Gadermaier, et al. "IgE and IgG4 Epitopes of Dermatophagoides and Blomia Allergens before and after Sublingual Immunotherapy." International Journal of Molecular Sciences 24, no. 4 (2023): 4173. http://dx.doi.org/10.3390/ijms24044173.

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Sublingual immunotherapy (SLIT) is used worldwide to treat house dust mites (HDM) allergy. Epitope specific immunotherapy with peptide vaccines is used far less, but it is of great interest in the treatment of allergic reactions, as it precludes the drawbacks of allergen extracts. The ideal peptide candidates would bind to IgG, blocking IgE-binding. To better elucidate IgE and IgG4 epitope profiles during SLIT, sequences of main allergens, Der p 1, 2, 5, 7, 10, 23 and Blo t 5, 6, 12, 13, were included in a 15-mer peptide microarray and tested against pooled sera from 10 patients pre- and post-

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Szameit, Sandra, Klemens Vierlinger, Letizia Farmer, Helga Tuschl, and Christa Noehammer. "Microarray-Based In Vitro Test System for the Discrimination of Contact Allergens and Irritants: Identification of Potential Marker Genes." Clinical Chemistry 54, no. 3 (2008): 525–33. http://dx.doi.org/10.1373/clinchem.2007.097386.

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Abstract Background: Animal tests have been used to characterize the potential of chemicals to produce allergic contact dermatitis, but this approach is increasingly a matter of public and political concern. Our aim was to develop and validate an alternative in vitro test that can identify contact allergens. Methods: We developed a targeted microarray containing oligonucleotide probes for 66 immune-relevant genes and analyzed gene expression in monocyte-derived dendritic cells (Mo-DCs) treated with 1 irritant (SDS) and 2 prominent contact allergens, nickel and Bandrowski’s base (BB), which is

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Orga-Dumitriu, Dan, Dana M. Harris, and Corina Porr. "Anaphylactic Shock Caused by Eating Buckwheat." Journal of Clinical Medicine 13, no. 17 (2024): 5243. http://dx.doi.org/10.3390/jcm13175243.

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Background: Urticaria is a common disease with a marked influence on quality of life. The key cell involved is the mast cell, which can be activated by a vast variety of stimuli, and the major mediator is histamine. Allergic urticaria is a disorder with a large variety of causes: food, drugs, insect venom, skin contact with allergens, and physical exercise. Buckwheat consumption has increased in European countries and the USA because it is gluten-free. It can trigger anaphylactic shock if ingested, inhaled, or handled with the hands. Five common buckwheat allergens named Fag e1 to 5 (Fag e1, 2

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Joshi, Amit A., Mark W. Peczuh, Challa V. Kumar, and James F. Rusling. "Ultrasensitive carbohydrate-peptide SPR imaging microarray for diagnosing IgE mediated peanut allergy." Analyst 139, no. 22 (2014): 5728–33. http://dx.doi.org/10.1039/c4an01544d.

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The first peptide-carbohydrate SPR imaging immunoarray aimed at diagnosing severity of peanut allergies is reported. The SPRi chip features a peptide epitope of peanut protein allergen Ara h2, a β-xylosyl glycoside, and anti-IgE antibody to achieve epitope-specific detection of human allergen IgEs at sub-attomol levels.

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Birras, Jasmin, Samuel J. White, Sigridur Jonsdottir, et al. "First clinical expression of equine insect bite hypersensitivity is associated with co-sensitization to multiple Culicoides allergens." PLOS ONE 16, no. 11 (2021): e0257819. http://dx.doi.org/10.1371/journal.pone.0257819.

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Background Insect bite hypersensitivity (IBH) is an IgE-mediated allergic dermatitis in horses incited by salivary allergens from Culicoides spp. IBH does not occur in Iceland, as the causative agents are absent, however a high prevalence is seen in horses exported to Culicoides-rich environments. Aims To study the natural course of sensitization to Culicoides allergens and identify the primary sensitizing allergen(s) in horses exported from Iceland utilizing a comprehensive panel of Culicoides recombinant (r-) allergens. Method IgE microarray profiling to 27 Culicoides r-allergens was conduct

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Dojcsák Kiss-Tóth, Éva, Dávid Németh, Adrienn J. Szalai, and Bertalan Fodor. "Molecular allergen and cross-allergen component studies among people living in the northheastern Hungarian region." Egészségtudományi Közlemények 14, no. 2 (2024): 44–59. https://doi.org/10.32967/etk.2024.027.

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Background: The availability of multiplex microarray-based diagnostic systems in the field of allergology contributes to the determination of personalized, component- based diagnosis. It enables allergen source and accurate allergen component identification even in patients with ambiguous symptoms and Prick skin test suggesting simultaneous positivity of two or more related allergens. Aim: In our study, we determined the presence and type of sensitization in the adult population of northeastern Hungary using a new type of multiple allergy diagnostic system and identified the molecular componen

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Halim, Timotheus, Ann Sun, and Fumio Takei. "Lung natural helper cells are an important source of innate TH2 cytokines (158.2)." Journal of Immunology 186, no. 1_Supplement (2011): 158.2. http://dx.doi.org/10.4049/jimmunol.186.supp.158.2.

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Abstract Recently, novel innate TH2 cytokine producing lymphocytes termed nuocytes or natural helper cells have been found in the gut-associated mucosa tissues. Similar TH2 cytokine producing innate cells have also been detected in other organs, including the lung, but their functional significance is not well understood. We have identified TH2 cytokine producing innate lymphocytes, termed lung natural helper (NH) cells in B6 mouse lungs and elucidated their role in lung eosinophilia and asthma. Lung NH cells are Lineage-negative but express of Sca-1, c-Kit, CD127 and CD25. Lung NH cell gene e

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Jahn-Schmid, B., C. Harwanegg, R. Hiller, et al. "Allergen microarray: comparison of microarray using recombinant allergens with conventional diagnostic methods to detect allergen-specific serum immunoglobulin E." Clinical & Experimental Allergy 33, no. 10 (2003): 1443–49. http://dx.doi.org/10.1046/j.1365-2222.2003.01784.x.

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Kalli, Marina, Andrew Blok, Marcos Alcocer, and Franco Falcone. "Protein microarrays: The future of allergy diagnosis? Optimization of coating and printing of allergen arrays used with fluorescent humanised basophil reporter cell line NFAT-DsRed RBL." World Allergy Organization Journal 13, no. 8 (2020): 100407. http://dx.doi.org/10.1016/j.waojou.2020.100407.

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Hsiao, Yun-Hsia, Charles Chen, and Ton Willemse. "Allergen Sensitization Patterns of Allergic Dogs: IgE-microarray Analysis." Thai Journal of Veterinary Medicine 46, no. 2 (2016): 235–42. http://dx.doi.org/10.56808/2985-1130.2731.

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Hochwallner, H., U. Schulmeister, I. Swoboda, et al. "Microarray and allergenic activity assessment of milk allergens." Clinical & Experimental Allergy 40, no. 12 (2010): 1809–18. http://dx.doi.org/10.1111/j.1365-2222.2010.03602.x.

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Mari, Adriano, Claudia Alessandri, Maria Livia Bernardi, Rosetta Ferrara, Enrico Scala, and Danila Zennaro. "Microarrayed Allergen Molecules for the Diagnosis of Allergic Diseases." Current Allergy and Asthma Reports 10, no. 5 (2010): 357–64. http://dx.doi.org/10.1007/s11882-010-0132-0.

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Preda, Mariana, Florin-Dan Popescu, Emilia Vassilopoulou, and Sylwia Smolinska. "Allergenic Biomarkers in the Molecular Diagnosis of IgE-Mediated Wheat Allergy." International Journal of Molecular Sciences 25, no. 15 (2024): 8210. http://dx.doi.org/10.3390/ijms25158210.

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IgE-mediated wheat allergy can take on various forms, including childhood food allergy to wheat, wheat-dependent exercise-induced anaphylaxis in young adults, baker’s respiratory allergy/asthma in workers exposed to wheat flour inhalation, and contact urticaria that is caused by hydrolyzed wheat proteins in some cosmetics, and that is sometimes associated with a food allergy. Singleplex and multiplex immunoassays detect specific IgE antibodies to wheat allergenic molecular biomarkers such as omega-5 gliadin Tri a 19, lipid transfer protein Tri a 14, and alpha-amylase inhibitors. The fluorescen

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Rudokas, Vytautas, Laimis Silimavicius, Indre Kucinskaite-Kodze, Aiste Sliziene, Milda Pleckaityte, and Aurelija Zvirbliene. "Novel monoclonal antibodies against house dust mite allergen Der p 21 and their application to analyze allergen extracts." PeerJ 12 (April 18, 2024): e17233. http://dx.doi.org/10.7717/peerj.17233.

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Background Allergen extracts and recombinant allergens are used in allergy diagnostics and immunotherapy. Since allergen extracts from different manufacturers lack proper standardization regarding their composition, monoclonal antibodies (MAbs) against specific allergen components can be used for their identification and quantification in allergen extracts. This study aimed to generate MAbs against allergen Der p 21 of Dermatophagoides pteronyssinus for the analysis of allergen extracts. Methods Recombinant Der p 21 was expressed in E. coli and purified using affinity chromatography. MAbs agai

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Moreira, Priscila Ferreira de Sousa, Katharina Gangl, Francisco de Assis Machado Vieira, et al. "Allergen Microarray Indicates Pooideae Sensitization in Brazilian Grass Pollen Allergic Patients." PLOS ONE 10, no. 6 (2015): e0128402. http://dx.doi.org/10.1371/journal.pone.0128402.

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Fortino, Vittorio, Lukas Wisgrill, Paulina Werner, et al. "Machine-learning–driven biomarker discovery for the discrimination between allergic and irritant contact dermatitis." Proceedings of the National Academy of Sciences 117, no. 52 (2020): 33474–85. http://dx.doi.org/10.1073/pnas.2009192117.

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Contact dermatitis tremendously impacts the quality of life of suffering patients. Currently, diagnostic regimes rely on allergy testing, exposure specification, and follow-up visits; however, distinguishing the clinical phenotype of irritant and allergic contact dermatitis remains challenging. Employing integrative transcriptomic analysis and machine-learning approaches, we aimed to decipher disease-related signature genes to find suitable sets of biomarkers. A total of 89 positive patch-test reaction biopsies against four contact allergens and two irritants were analyzed via microarray. Coex

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Abdurasulova, Tajixol Ramazanovna, Charos Luqmonovna Davronova, and Sunnat Yusufjon o'g'li. O'razaliyev. "REALITY OF MODERN ALLERGOLOGY, ALLERGY DIAGNOSTICS." Multidisciplinary Journal of Science and Technology 3, no. 4 (2023): 42–47. https://doi.org/10.5281/zenodo.10089712.

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Abstract (sommario):

The article provides a review of the literature on current and promising directious for the identification and treatment of allergic diseases. Furthermore, the component resolved allergy diagnosis and the use of microarray technology – ISAC – are being considered to determine the level of IgE antibodies to various allergenic molecules. 

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Doyen, Virginie, Carine Truyens, Hoa Nhu Thi, et al. "Helminth infection induces non-functional sensitization to house dust mites." PLOS ONE 16, no. 7 (2021): e0253887. http://dx.doi.org/10.1371/journal.pone.0253887.

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Abstract (sommario):

Background IgE characterizes the humoral response of allergic sensitization but less is known about what modulates its function and why some patients present clinical symptoms for a given IgE level and others do not. An IgE response also occurs during helminth diseases, independently of allergic symptoms. This response could be a model of non-functional IgE. Objective To study the IgE response against environmental allergens induced during natural helminth infection. Methods In 28 non allergic subjects from the periphery of Ho Chi Minh city with (H+, n = 18) and without helminth infection (H-,

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46

Veramendi-Espinoza, Liz. "Wheat-dependent exercise-induced anaphylaxis: The diagnostic utility of Omega-5 Gliadin in two clinical cases from Lima, Peru." Revista Alergia México 72, no. 2 (2025): 142–46. https://doi.org/10.29262/ram.v72i2.1455.

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Abstract (sommario):

Background: Wheat-dependent exercise-induced anaphylaxis (WDEIA) is a rare, potentially life-threatening condition triggered by wheat ingestion followed by physical activity. Case report: This report describes two cases of young individuals in Lima, Peru, diagnosed with WDEIA. The diagnosis was confirmed through skin prick testing and the Allergy Explorer2 (ALEX-2) microarray, identifying omega-5gliadin (Tria19) as the main allergen. Both patients experienced episodes of anaphylaxis, one of which required adrenaline administration. Conclusion: The importance of molecular diagnostics to confirm

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Sena-Torralba, Amadeo, Javier Gabaldón-Atienza, Aitor Cubells-Gómez, Patricia Casino, Ángel Maquieira, and Sergi Morais. "Lateral Flow Microimmunoassay (LFµIA) for the Reliable Quantification of Allergen Traces in Food Consumables." Biosensors 12, no. 11 (2022): 980. http://dx.doi.org/10.3390/bios12110980.

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Abstract (sommario):

Quality assurance and food safety are of great concern within the food industry because of unknown quantities of allergens often present in food. Therefore, there is an ongoing need to develop rapid, sensitive, and easy to use methods that serve as an alternative to mass spectrometry and enzyme-linked immunosorbent assay (ELISA) for monitoring food safety. Lateral flow immunoassay is one of the most used point-of-need devices for clinical, environmental, and food safety applications. Compared to traditional methods, it appears to be a simple and fast alternative for detecting food allergens. H

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polikepahad, sumanth, Jad El-Daye, Arash Naghavi, Jonathan Miller, Preethi Gunaratne, and David Brian Corry. "Lung RNA profiling suggests an essential role for micro RNAs in regulating allergic lung disease (91.9)." Journal of Immunology 178, no. 1_Supplement (2007): S161. http://dx.doi.org/10.4049/jimmunol.178.supp.91.9.

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Abstract (sommario):

Abstract MicroRNAs (miRNAs) are small non-coding RNAs, which negatively regulate gene expression by translational inhibition of target mRNAs. In vertebrates, miRNAs are produced in most organs, including lungs. In the current study, we hypothesized that the expression of miRNAs would correlate inversely with expression of critically important target genes involved in the pathogenesis of allergic lung disease. After intranasal challenge of mice with a potent allergen derived from Aspergillus oryzae on alternating days for 2 weeks, lungs were isolated, high quality RNA was extracted and subjecte

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Lupinek, Christian, Eva Wollmann, and Rudolf Valenta. "Monitoring Allergen Immunotherapy Effects by Microarray." Current Treatment Options in Allergy 3, no. 2 (2016): 189–203. http://dx.doi.org/10.1007/s40521-016-0084-2.

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Sano, Akiyo, Akiko Yagami, Kayoko Suzuki, et al. "Two Cases of Occupational Contact Urticaria Caused by Percutaneous Sensitization to Parvalbumin." Case Reports in Dermatology 7, no. 2 (2015): 227–32. http://dx.doi.org/10.1159/000439080.

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Abstract (sommario):

Background: In recent years, it has been proposed that the primary mechanism for the development of food allergies is percutaneous sensitization. Since 2010, in Japan, the number of immediate-type wheat allergy due to hydrolyzed wheat protein has dramatically increased among those who have been using soap containing hydrolyzed wheat. This incidence supports the hypothesis that food allergens arise through percutaneous sensitization. Clinical Summary: A 25-year-old man (case 1) and an 18-year-old girl (case 2) with atopic dermatitis visited our Department because of food allergy and hand eczema

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