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Tesi sul tema "Biosynthesis"

Autore: Grafiati

Pubblicato: 4 giugno 2021

Ultima modifica: 25 luglio 2025

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1

Eyles, Tom. "Biosynthetic Lego : reprogramming RiPP biosynthesis." Thesis, University of East Anglia, 2018. https://ueaeprints.uea.ac.uk/69571/.

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Abstract (sommario):

Ribosomally synthesised and post translationally modified peptides (RiPPs) are a diverse class of industrially-important and clinically-relevant natural products. Reprogramming the biosynthesis of RiPPs can provide an understanding of their biosynthesis, increases in their yield, and compound derivatives. In this thesis, two RiPP biosynthetic pathways are reprogrammed to achieve these aims. Bottromycin is a potent antibiotic RiPP, however it is produced in low yields by its native producer and it is rapidly hydrolysed in blood plasma. It was hypothesised that the bottromycin gene cluster could

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2

Khairudin, Khairunisa. "Biosynthetic studies and combinatorial biosynthesis of pleuromutilin antibiotics." Thesis, University of Bristol, 2018. http://hdl.handle.net/1983/46271504-0b2b-457a-92d0-073885f512cd.

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Pleuromutilin has potential as a next-generation antibiotic, and many semi-synthetic pleuromutilin derivatives have been developed. Recently, characterization of individual enzymatic steps involved in the production of pleuromutilin has been carried out. A linear pathway of pleuromutilin biosynthesis was established; however, there is a possibility of alternative or shunt pathways. Thus, the first part of this thesis aimed to investigate if any other possible routes could lead to the biosynthesis of pleuromutilin. Two alternative pathways were identified from the expression of various combinat

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3

Walczak, Robbie J. "Analyses of antibiotic biosyntheses in Streptomyces spp. : the molecular biology of nonactin biosynthesis and the novel biochemistry of daunorubicin biosynthesis /." The Ohio State University, 2001. http://rave.ohiolink.edu/etdc/view?acc_num=osu1488205318510564.

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4

Gray, Jennifer A. "Biotin biosynthetic enzymes and the metabolic control of biotin biosynthesis." [Ames, Iowa : Iowa State University], 2009. http://gateway.proquest.com/openurl?url_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:dissertation&res_dat=xri:pqdiss&rft_dat=xri:pqdiss:1473213.

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5

Jackson, Catherine Mary. "Tetronasin biosynthesis." Thesis, University of Cambridge, 1988. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.303274.

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6

Jacobs, Adam. "Aspyrone biosynthesis." Thesis, University of Cambridge, 1992. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.241064.

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7

Ndiege, Isaiah Omolo. "Polyketide biosynthesis." Thesis, University of Cambridge, 1989. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.315331.

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8

Suzuki, Shiro. "Stereochemical diversity in lignan biosynthesis and establishment of norlignan biosynthetic pathway." Kyoto University, 2002. http://hdl.handle.net/2433/78141.

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Kyoto University (京都大学)<br>0048<br>新制・課程博士<br>博士(農学)<br>甲第9652号<br>農博第1280号<br>新制||農||848(附属図書館)<br>学位論文||H14||N3684(農学部図書室)<br>UT51-2002-G410<br>京都大学大学院農学研究科応用生命科学専攻<br>(主査)教授島田幹夫, 教授桒原保正, 教授坂田完三<br>学位規則第4条第1項該当

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9

Purvis, Michael Bernard. "Stereochemical aspects of virginiamycin biosynthesis: biosynthesis of antibiotic A33853." Diss., Virginia Polytechnic Institute and State University, 1989. http://hdl.handle.net/10919/54266.

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Abstract (sommario):

The biochemical pathways for the formation of the unusual amino acids found in virginiamycin M₁ and A33853 were investigated. Specifically tritiated and carbon 14 labeled serines were incorporated into virginiamycin M₁. (2S)-serine and (2S,3R)-[3-³H] serine were found to be precursors, thus giving evidence of stereochemical control in the formation of the oxazole moiety. This information allowed for postulation of a ring closure pathway. Stereochemical investigations were also carried out on the dehydroproline unit and it was shown that both (R) and (S) prolines were incorporated into the dehy

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10

Milne, Keith Livingston. "Bacterial isoprenoid biosynthesis." Thesis, University of Edinburgh, 1990. http://hdl.handle.net/1842/11172.

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Abstract (sommario):

This thesis describes a possible alternative isoprenoid pathway in bacteria by considering some previously unpublished feeding studies in the context of the related background literature. Three synthetic routes to 2,4-dihydroxy-4-methyltetrahydropyran (63) and three synthetic strategies towards the synthesis of 2-carboxy-2,4-dihydroxy-4-methyltetrahydropyran (63) are discussed. These compounds are considered as potential intermediates in the proposed alternative bacterial isoprenoid pathway. Labelled synthesis of (63) and structural analysis of (63) and 4-hydroxy-2-methoxy-4-methyltetrahydropy

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11

Milne, Claire E. "Biosynthesis and engineered biosynthesis of Calcium Dependent Antibiotic (CDA) from streptomyces coelicolor." Thesis, University of Manchester, 2004. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.506586.

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Abstract (sommario):

Calcium Dependent Antibiotic (CDA), is a nonribosomal lipopeptide antibiotic from Streptomyces coelicolor. It is an undecapeptide with an unusual N-terminal 2,3-epoxyhexanoyl fatty acid side chain. It contains many nonproteinogenic amino acids such as D-4-hydroxyphenylglycine, D-3-phosphohydroxyasparagien, L-3-methylglutamic acid and Z-2,3-dehydrotryptophan. CDA is very similar in structure to the antibiotic daptomycin, which under the trade name Cubicin has recently been approved for the treatment of skin infections. The work described in this thesis focuses on a number of different areas, wi

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12

Schildknecht, Stefan. "Redoxregulation of prostanoid biosynthesis." [S.l.] : [s.n.], 2005. http://deposit.ddb.de/cgi-bin/dokserv?idn=976070073.

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13

Wicher, Krzysztof B. "Haptoglobin: Biosynthesis and Evolution." Doctoral thesis, Uppsala University, Department of Medical Biochemistry and Microbiology, 2006. http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-6446.

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<p>Haptoglobin (Hp) is a serum protein known for its ability to form a tight complex with hemoglobin (Hb) and thereby inhibiting the oxidative activity of Hb. </p><p>Mammalian Hp is synthesized as a precursor (proHp) that undergoes proteolytic cleavage by a previously unidentified enzyme in the endoplasmic reticulum (ER). In this study, a proHp-cleaving enzyme was isolated from human serum and identified as complement C1r-like protein (C1rLP). Co-expression of C1rLP with proHp in mammalian cells resulted in cleavage of the latter protein in the ER. Mutation of either the active site serine res

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14

Kollmeyer, Jessica Elaine. "Regulation of Galactosylceramide Biosynthesis." Thesis, Georgia Institute of Technology, 2006. http://hdl.handle.net/1853/11630.

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An important branchpoint of mammalian sphingolipid metabolism occurs at the step where ceramides are glycosylated to glucosylceramide (GlcCer) versus galactosylceramide (GalCer), which are precursors of all mammalian glycosphingolipids. Relatively few studies have focused on this branchpoint because these monohexosylceramides are somewhat difficult to resolve chromatographically and because molecular biology tools have only recently become available to follow expression of these genes. The goal of this thesis is to better understand the mechanisms of cell regulation determining galactosylcer

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15

Ross, Timothy Kieran. "Regulation of polyglycerophospholipid biosynthesis." Thesis, National Library of Canada = Bibliothèque nationale du Canada, 1998. http://www.collectionscanada.ca/obj/s4/f2/dsk2/ftp01/MQ32238.pdf.

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16

Lewis, Elizabeth A. "The biosynthesis of chloramphenicol." Thesis, National Library of Canada = Bibliothèque nationale du Canada, 1998. http://www.collectionscanada.ca/obj/s4/f2/dsk2/ftp01/MQ36494.pdf.

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17

Keyes, Robert F. "The biosynthesis of ravidomycin." Thesis, This resource online, 1989. http://scholar.lib.vt.edu/theses/available/etd-08252008-162246/.

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18

Lewis, C. N. "The biosynthesis of canescin." Thesis, University of Cambridge, 1986. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.373266.

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19

Loughran, Mark Stephen. "The biosynthesis of erythromycin." Thesis, University of Cambridge, 1994. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.307943.

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20

Andrews, Timothy Stephen. "The biosynthesis of erythromycin." Thesis, University of Cambridge, 1990. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.358365.

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21

Lipscomb, Sarah. "Studies on cephalosporin biosynthesis." Thesis, University of Oxford, 2002. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.433559.

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22

Lee, Hwei-Jen. "Studies on cephalosporin biosynthesis." Thesis, University of Oxford, 1999. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.409949.

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23

Lloyd, Matthew David. "Biosynthesis of calavulanic acid." Thesis, University of Oxford, 1992. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.294290.

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24

Sleeman, Mark. "Studies on carbapenem biosynthesis." Thesis, University of Oxford, 2005. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.432411.

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25

Rackham, Emma Jayne. "Elucidation of pacidamycin biosynthesis." Thesis, University of East Anglia, 2011. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.539367.

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26

Hardick, David James. "The biosynthesis of nojirimycins." Thesis, University of Warwick, 1992. http://wrap.warwick.ac.uk/4419/.

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Abstract (sommario):

Streptomyces subrutilus ATCC 27467, when grown on a glucosecontaining soyabean medium, produces both 1-deoxymannonojirimycin (DMJ) and 1-deoxynojirimycin (DNJ) in its culture medium. When 1- or 2-[2H]-D-glucose is used, the deuterium label appears at C6 in both alkaloids and the labelling pattern suggests that the first step in the biosynthesis of both DNJ and DMJ is a glucose to fructose isomerisation. Studies with 5-[2H]-D-glucose and 6,6-[2H2]-D-glucose indicate that oxidation of the 6-position of the glucose/ fructose occurs during the biosynthesis and that mannonojirimycin is the first am

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27

Watson, A. J. "Aflatoxin biosynthesis in Aspergillus." Thesis, University of East Anglia, 1998. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.267259.

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28

Sanders, M. "Experiments in rotenoid biosynthesis." Thesis, University of Nottingham, 1987. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.376182.

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29

MacLachlan, W. S. "The biosynthesis of cryptosporiopsinol." Thesis, University of Glasgow, 1985. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.375450.

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30

Lam, Wai Ho. "Biosynthesis of Mureidomycin A." Thesis, University of Warwick, 2006. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.444836.

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31

Browne, M. "Carotenoid biosynthesis in bacteria." Thesis, University of Liverpool, 1986. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.372685.

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32

Clark, C. A. "The biosynthesis of nonactin." Thesis, University of Southampton, 1985. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.370333.

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33

Sparkes, Andrew Windsor. "Studies of archaemycin biosynthesis." Thesis, University of Southampton, 1993. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.239645.

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34

Gordon, J. "The biosynthesis of aphidicolin." Thesis, University of Sussex, 1986. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.375170.

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35

McAndrew, Douglas. "Studies on polyketide biosynthesis." Thesis, University of Bristol, 1996. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.337292.

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36

Demetriadou, A. K. "The biosynthesis of heptaketides." Thesis, University of Cambridge, 1985. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.355259.

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37

Hill, Alison Margaret. "The biosynthesis of aspyrone." Thesis, University of Cambridge, 1994. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.319492.

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38

Di, Marzo V. "Neuropeptides and leukotriene biosynthesis." Thesis, Imperial College London, 1988. http://hdl.handle.net/10044/1/47024.

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39

Gómez, Velasco Anaximandro. "Studies in mycobactin biosynthesis." Thesis, University of Birmingham, 2009. http://etheses.bham.ac.uk//id/eprint/8030/.

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Abstract (sommario):

Tuberculosis (TB) is the leading cause of infectious disease mortality in the world by a single bacterial pathogen, Mycobacterium tuberculosis. Current TB chemotherapy remains useful in treating susceptible M. tuberculosis strains, however, the emergence of MDR-TB and XDR-TB demand the development of new drugs. Enzymes involved in mycobactin biosynthesis, low molecular weight iron chelators, do not have mammalian homologues; therefore they are considered potential targets for the development of new anti-TB drugs. The aims of this study were to identify potential inhibitors and to investigate t

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40

Kirkpatrick, Peter Neil. "The biosynthesis of chloroeremomycin." Thesis, University of Cambridge, 2000. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.621622.

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41

Filipek-Górniok, Beata. "Glycosaminoglycan Biosynthesis in Zebrafish." Doctoral thesis, Uppsala universitet, Institutionen för medicinsk biokemi och mikrobiologi, 2015. http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-264269.

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Abstract (sommario):

Proteoglycans (PGs) are composed of highly sulfated glycosaminoglycans chains (GAGs) attached to specific core proteins. They are present in extracellular matrices, on the cell surface and in storage granules of hematopoietic cells. Heparan sulfate (HS) and chondroitin/dermatan sulfate (CS/DS) GAGs play indispensable roles in a wide range of biological processes, where they can serve as protein carriers, be involved in growth factor or morphogen gradient formation and act as co-receptors in signaling processes. Protein binding abilities of GAGs are believed to be predominantly dependent on the

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42

Donovan, Tessa May. "Biosynthesis of fungal melanin." Thesis, University of Edinburgh, 1989. http://hdl.handle.net/1842/13689.

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43

Ramos, Tania. "Cysteine biosynthesis in Leishmania." Thesis, University of Glasgow, 2014. http://theses.gla.ac.uk/5156/.

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Abstract (sommario):

Every year 2 million people are diagnosed with leishmaniasis and 350 million are at risk of becoming infected. Spread throughout 88 countries in the world, leishmaniasis is a group of diseases comprising visceral, mucocutaneous and cutaneous leishmaniasis as the main forms. Visceral leishmaniasis is the most severe form of the disease and is caused by Leishmania donovani. The parasite, Leishmania, is transmitted to humans by a sandfly vector. The absence of vector-control procedures and effective vaccines for humans makes chemotherapy the only weapon against leishmaniasis. Great efforts have b

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44

Reva, Anna. "Enzymology of gentamicin biosynthesis." Thesis, University of Cambridge, 2018. https://www.repository.cam.ac.uk/handle/1810/277902.

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Abstract (sommario):

Gentamicin C complex is a mixture of five structurally similar aminoglycoside antibiotics, gentamicins C1, C1a, C2, C2a, and C2b, produced by the actinomycete bacterium Micromonospora echinospora. It is established in clinical use and despite significant toxicity remains valuable to treat severe Gram-negative bacterial infections. There is a pressing need to develop novel versions of such antibiotics to combat the rise of resistance among pathogens. Engineering of the pathway requires a detailed knowledge of the genes, enzymes, and intermediates involved. The final steps of gentamicin biosynth

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45

Martins, Filipa Teixeira. "Studies of thiamine biosynthesis." Thesis, University of Southampton, 2009. https://eprints.soton.ac.uk/71854/.

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Abstract (sommario):

In Escherichia coli, and other prokaryotes, thiamine (vitamin B1) is assembled by coupling 4-amino-5-hydroxymethyl-2-methylpyrimidine pyrophosphate (Hmp-PP) and 4-methyl-5-(β-hydroxyethyl)thiazole phosphate (Thz-P). The thiazole moiety is biosynthesised from 1-deoxyxylulose-5-phosphate, tyrosine, and cysteine, and at least six genes are required including thiH, thiG, thiS, and thiF. Whilst in aerobes, the C2-N3 fragment of Thz-P derives from glycine in a reaction catalysed by the flavoenzyme ThiO, in anaerobes such as E. coli, dehydroglycine is formed from tyrosine in a ThiH dependent reaction

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46

Khaw, Lake Ee. "The biosynthesis of rapamycin." Thesis, University of Cambridge, 1995. https://www.repository.cam.ac.uk/handle/1810/272287.

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47

Singh, Neena. "Biosynthesis of glycophospholipid anchors." Case Western Reserve University School of Graduate Studies / OhioLINK, 1990. http://rave.ohiolink.edu/etdc/view?acc_num=case1054926816.

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48

Cho, Hyeongjin. "Studies in antibiotics biosynthesis /." The Ohio State University, 1990. http://rave.ohiolink.edu/etdc/view?acc_num=osu1487676847114716.

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49

Simpson, T. J. "Biosynthesis of fungal metabolites." Thesis, University of Edinburgh, 1986. http://hdl.handle.net/1842/11389.

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50

Fugelstad, Johanna. "Cellulose Biosynthesis in Oomycetes." Licentiate thesis, Stockholm : KTH Biotechnology, 2008. http://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-9282.

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