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Letteratura scientifica selezionata sul tema "Botrytis cinerea"

Autore: Grafiati

Pubblicato: 4 giugno 2021

Ultima modifica: 25 luglio 2025

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Articoli di riviste sul tema "Botrytis cinerea"

Chen, Tong, Zhanquan Zhang, Yong Chen, Boqiang Li, and Shiping Tian. "Botrytis cinerea." Current Biology 33, no. 11 (2023): R460—R462. http://dx.doi.org/10.1016/j.cub.2023.01.058.

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Vinogradova, Svetlana, Elena Porotikova, Emiliya Navrotskaya, Zsuzsanna Nagyne Galbacs, Sébastien Massart, and Eva Varallyay. "The First Virome of a Russian Vineyard." Plants 12, no. 18 (2023): 3292. http://dx.doi.org/10.3390/plants12183292.

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Among other pathogens, more than 80 viruses infect grapevine. The aim of this work was to study the virome diversity of grapevine viruses and mycoviruses of a vineyard using high-throughput sequencing technologies. The grapevine virome was studied in symptomatic vines of the Rkatsiteli cultivar (V. vinifera) collected at the vineyards of the Krasnodar Krai in Russia. Ribosomal-depleted total RNA and isolated small RNAs were used for library preparation and high-throughput sequencing. Six grapevine-infecting viruses and two viroids were validated by RT-PCR and analyzed phylogenetically. We iden

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Guetsky, Ruth, D. Shtienberg, Y. Elad, E. Fischer, and A. Dinoor. "Improving Biological Control by Combining Biocontrol Agents Each with Several Mechanisms of Disease Suppression." Phytopathology® 92, no. 9 (2002): 976–85. http://dx.doi.org/10.1094/phyto.2002.92.9.976.

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Two biocontrol agents, a yeast (Pichia guilermondii) and a bacterium (Bacillus mycoides), were tested separately and together for suppression of Botrytis cinerea on strawberry leaves and plants. Scanning electron microscopy revealed significant inhibition of Botrytis cinerea conidial germination in the presence of Pichia guilermondii, whereas Bacillus mycoides caused breakage and destruction of conidia. When both biocontrol agents were applied in a mixture, conidial destruction was more severe. The modes of action of each of the biocontrol agents were elucidated and the relative quantitative c

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Adjebli, Ahmed, Abdelaziz Messis, Riad Ayeche, and Kamel Aissat. "Phenotypic variability of Botrytis cinerea and Botrytis pseudocinerea isolates." Research Journal of Biotechnology 17, no. 3 (2022): 20–26. http://dx.doi.org/10.25303/1703rjbt2026.

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In the present study, eight single-spore strains of Botrytis cinerea were isolated from tomato greenhouses located in Bejaia regions (Northern Algeria). Isolates were molecularly characterized by nine microsatellite markers. Isolates were assigned to B. cinerea and B. pseudocinerea with four isolates of each species. Morphological characterization was established using two cultures media Potato Dextrose Agar and Malt Extract Agar. All isolates inoculated on PDA medium were exclusively Sclerotial and Mycelial on MEA medium. Aggressiveness of both species was similar on tomato leaves and apple f

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Qiu, Lu, Hai Han Yang, Fang Lei, Shu Guo Fan, Mei Hua Xie, and Zhen Ji Wang. "Studies on the Bacteriostasis of Nano-Silver on the Pathogenic Fungus Botrytis cinerea from Illed Plants." Applied Mechanics and Materials 651-653 (September 2014): 352–61. http://dx.doi.org/10.4028/www.scientific.net/amm.651-653.352.

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Obiective is studing the bacteriostasis of nanosilver on the pathogenic fungus Botryticinerea from illed plants. Five strains of Botrytis cinerea were used as the experimental materials. 0.3 % carbendazim and 0.3 % chlorothalonil were used as comparing chemistry bacteriostatic agents. The inhibitionand effects of bacteriostatic agents on the growth of mycelia, spore’s germination, size of the inhibitory zone, electrical conductivity, morphology and structure of Botrytis cinerea were studied. Results is that the bacteriostatic effects of nanosilver is significantly better than blank comparing e

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Chetelat, R. T., and L. Stamova. "TOLERANCE TO BOTRYTIS CINEREA." Acta Horticulturae, no. 487 (March 1999): 313–16. http://dx.doi.org/10.17660/actahortic.1999.487.48.

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Guerrero Prieto, Víctor Manuel, Juan Luis Jacobo Cuéllar, Rafael Ángel Parra Quezada, et al. "Botrytis cinerea Pers. in postharvest apple fruit, control with Candida oleophila Montrocher strains and/or synthetic fungicides." Nova Scientia 11, no. 22 (2019): 69–84. http://dx.doi.org/10.21640/ns.v11i22.1645.

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As an alternative control method, to improve control and to reduce synthetic fungicide use, three Candida oleophila strains and/or four commercial synthetic fungicides were used to control Botrytis cinerea damage on postharvest apple fruit. Synthetic commercial fungicides; Cyprodinil+Fludioxonil, Thiabendazole and Benomyl, allowed Candida oleophila strains colony growth when challenged to the pressure of these fungicides. Synthetic commercial fungicide Captan did not allow any Candida oleophila strains colony growth. Control of Botrytis cinerea expressed in % of damage and damage reduction, ga

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Komalaningrat, Devi ayu, Efi Toding Tondok, and Widodo Widodo. "Identitas Spesies Botrytis pada Tanaman Hortikultura Di Jawa Barat, Indonesia." Jurnal Fitopatologi Indonesia 14, no. 6 (2019): 205. http://dx.doi.org/10.14692/jfi.14.6.205.

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Identity of Botrytis Species on Horticultural Crops In West Java, IndonesiaBotrytis species are economically important pathogens with a very broad host range including more than 200 horticultural crops. The identity of these fungus found in Indonesia has not been investigated and need to be reconfirmed due to the species variations of Botrytis found worldwide. The aims of this research were to identify Botrytis species infecting crops in West Java based on its morphology and molecular characteristics, as well as its pathogenicity traits. Based on morphological characters, all 25 isolates found

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Ripardo-Filho, Haroldo da Silva, Víctor Coca Ruíz, Ivonne Suárez, Javier Moraga, Josefina Aleu, and Isidro G. Collado. "From Genes to Molecules, Secondary Metabolism in Botrytis cinerea: New Insights into Anamorphic and Teleomorphic Stages." Plants 12, no. 3 (2023): 553. http://dx.doi.org/10.3390/plants12030553.

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The ascomycete Botrytis cinerea Pers. Fr., classified within the family Sclerotiniaceae, is the agent that causes grey mould disease which infects at least 1400 plant species, including crops of economic importance such as grapes and strawberries. The life cycle of B. cinerea consists of two phases: asexual (anamorph, Botrytis cinerea Pers. Fr.) and sexual (teleomorph, Botryotinia fuckeliana (de Bary) Wetzel). During the XVI International Symposium dedicated to the Botrytis fungus, which was held in Bari in June 2013, the scientific community unanimously decided to assign the most widely used

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Ahmed, AU, S. Zaman, MA Mazid, et al. "Studies of Botrytis cinerea causing botrytis gray mold disease in chickpea (Cicer arietinum L.)." Journal of Bio-Science 22 (October 21, 2016): 69–76. http://dx.doi.org/10.3329/jbs.v22i0.30011.

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Context: To investigate the morphological and pathological information on causal organism Botrytis cinerea for better understanding of the infection process and controlling outbreaks of the most damaging disease, Botrytis Gray Mold (BGM),of chickpea.Objectives: To study the cultural and morphological characteristics and growth requirements of Botrytis cinerea Pers. ex. Fr. This study was also aimed to know about the cytological and genetic behavior of B. cinerea in relation to its pathogenicity and the infection process on chickpea.Materials and Methods: A total of 83 isolates of Botrytis cine

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Tesi sul tema "Botrytis cinerea"

Shafia, Aminath. "Latent infection of Botrytis cinerea." Thesis, University of Reading, 2009. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.499372.

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Latent B. cinerea was detected in nine symptomless wild host species from the families Asteraceae and Brassicaceae, in addition to greenhouse grown lettuce. Conventional testing methods revealed that latent B. cinerea was equally prevalent in the root system as the above ground parts. Incidence of latent infection was moderate in some species (Achillea milleforlium, Arabidopsis thaliana, Centraurea nigra, Cirsium vulgare, Senecio jacobaea, Senecio vulgaris and Taraaxacum agg.) and rare in others (Tussilago farfara and Bellis perennis). In greenhouse lettuce, latent infection was activated by p

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Lewis, Megan. "The flavohaemoglobins of Botrytis cinerea." Thesis, University of Sheffield, 2009. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.521869.

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Rajaguru, Bulathsinhalage Anuja Priyangani. "Molecular ecology of Botrytis cinerea." Thesis, University of Reading, 2008. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.494963.

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Systemic Botrytis cinerea isolates were collected from non symptomatic fruits of Rubus fruticosus (blackberry) and Fragaria x ananassae (strawberry) and from roots and leaves of wild Primula vulgaris (primrose) and Taraxacum agg. (dandelion) in Brighton, Bath, and Reading separated by 80km or more. Isolates recovered from a Primula x polyantha crop at Reading were also tested. Approximately 300 isolates were genetically characterised using 9 published microsatellite primers and the presence of two transposable elements, boty and flipper. In wild primula and dandelion, incidence of non-symptoma

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Emmanuel, C. J. "'Symptomless' infection by Botrytis cinerea." Thesis, University of Reading, 2016. http://centaur.reading.ac.uk/63176/.

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The study was carried out to clarify the nature of symptomless infection by Botrytis cinerea and to what extent it differs from aggressive necrotic infection in Lactuca sativa (lettuce) and Arabidopsis thaliana. Symptomless plants were produced by dry spore inoculation in plants growing in controlled environmental conditions or in glasshouses. Plating out of surface-disinfected and non-surface-disinfected samples of inoculated, apparently healthy, plants on selective medium revealed that the fungus was spreading from the initial inoculation site to newly developing plant organs both internally

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Biosa, Carlotta. "Botrytis cinerea e la sua forma nobile." Bachelor's thesis, Alma Mater Studiorum - Università di Bologna, 2020.

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Il presente elaborato descrive gli aspetti generali di Botrytis cinerea quando si sviluppa come muffa grigia e muffa nobile. La differenza fra queste due diverse forme non è data da una differenza genetica del fungo ma dal microclima presente nell'area di infezione. Infatti l'alternanza di condizioni umide a condizioni secche fa si che il fungo si sviluppi solo all'interno dello strato epidermico (acino infavato) senza sviluppare efflorescenze all'esterno dell'acino (muffa grigia).La presenza della muffa apporta all'acino una maggiore concentrazione di solidi solubili, un lieve innalzamento di

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Swadling, Iain. "Biological control of Botrytis cinerea in strawberries." Thesis, University of Kent, 1994. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.240120.

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Deligeorgopoulou, Athina. "Sesquiterpenoids and their biotransformation by Botrytis cinerea." Thesis, University of Sussex, 2002. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.392802.

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Bratt, Richard P. "Spoilage of senescing flax by Botrytis cinerea Pers." Thesis, Queen's University Belfast, 1987. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.317056.

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Fernandez, Olivier. "Métabolisme du tréhalose chez la vigne (Vitis vinifera L.) en conditions stressantes : effets du froid et de l’infection par Botrytis cinerea." Thesis, Reims, 2011. http://www.theses.fr/2011REIMS016/document.

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L’objectif de mes travaux de thèse était d’étudier le métabolisme du tréhalose chez la vigne (Vitis vinifera L. cv. Chardonnay) en réponse à l’exposition à deux stress : le froid" chilling " et l’infection par le champignon pathogène Botrytis cinerea.Pour cela, nous avons tout d’abord optimisé un dosage du tréhalose par fluorimétrie qui nous a permis de caractériser le métabolisme du tréhalose en réponse à ces deux stress.Le métabolisme du tréhalose est activé différemment par le froid dans les organes dela vigne. Les gènes VvTPPA, codant une enzyme de synthèse du tréhalose, et VvTRE, codantla

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Barnes, Sally Elissa. "The epidemiology of Botrytis cinerea on greenhouse grown ornamentals." Thesis, University of Reading, 2002. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.394420.

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Libri sul tema "Botrytis cinerea"

Johnson, Dennis A. Botrytis bunch rot of grape. Cooperative Extension, College of Agriculture & Home Economics, Washington State University, 1986.

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Johnson, Dennis A. Botrytis neck rot of onion. Cooperative Extension, College of Agriculture & Home Economics, Washington State University, 1986.

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Aljourmi, Ismail. Characterization and protein fingerprinting of Botrytis cinerea isolates. Brock University, Dept. of Biological Sciences, 1999.

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James, Robert L. Resistance of Botrytis cinerea to vinclozolin, iprodione and dicloran. USDA Forest Service, Northern Region, Cooperative Forestry and Pest Management, 1985.

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Leone, Gionata. Significance and role of polygalacturonase production by Botrytis cinerea in pathogenesis. [s.n.], 1990.

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International Botrytis Symposium (10th 1992 Crete, Greece). Recent advances in Botrytis research: Proceedings of the 10th International Botrytis Symposium, Heraklion, Crete, Greece, 5-10 April 1992. Edited by Verhoeff K, Malathrakis N. E, and Williamson B. Pudoc Scientific Publishers, 1992.

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Peterson, Michael James. Grey mould control on container-grown Douglas-fir seedlings: Timing of fungicide application related to greenhouse environment. Forestry Canada, 1988.

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Peterson, Michael James. Grey mould control by seedling canopy humidity reduction through under-bench ventiliation and styroblock aeration. Forestry Canada, 1989.

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Variabilität und Pathogenität bei Botrytis cinerea. J. Cramer, 1999.

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Gligor, Bojkov. Gray Mold (Botrytis Cinerea) at Vines. Scientific Research Publishing, 2022.

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Capitoli di libri sul tema "Botrytis cinerea"

De Miccolis Angelini, Rita Milvia, Stefania Pollastro, and Franco Faretra. "Genetics of Botrytis cinerea." In Botrytis – the Fungus, the Pathogen and its Management in Agricultural Systems. Springer International Publishing, 2015. http://dx.doi.org/10.1007/978-3-319-23371-0_3.

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Claus, Harald. "Laccases of Botrytis cinerea." In Biology of Microorganisms on Grapes, in Must and in Wine. Springer International Publishing, 2017. http://dx.doi.org/10.1007/978-3-319-60021-5_14.

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Tudzynski, Bettina, and Christian Schulze Gronover. "Signalling in Botrytis cinerea." In Botrytis: Biology, Pathology and Control. Springer Netherlands, 2007. http://dx.doi.org/10.1007/978-1-4020-2626-3_6.

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Marois, James J. "Biological Control of Botrytis Cinerea." In Biological Control of Plant Diseases. Springer US, 1992. http://dx.doi.org/10.1007/978-1-4757-9468-7_15.

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Vykoupil, Libor. "Botrys neboli esej o záludnostech historikovy práce (rozprava o metodě)." In Filosofie jako životní cesta. Masaryk University Press, 2019. http://dx.doi.org/10.5817/cz.muni.p210-9458-2019-14.

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The elder and more experienced certainly know or at least have a vague idea that there used to be a Greek brandy named Botrys containing 40 % of alcohol. Its name was probably derived from the name of Botrytis cinerea (botrytis bunch rot, more commonly). The Greek term is Βότρυς and its transcription into Latin alphabet is Votrus or Votris. However, if a scholar attempts to verify in such an elementary finding, they can get entangled in very complex and tricky historical facts. After weeks of hard work it turned out that it is probably easier to write a chapter on the history of Greek economy

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Dik, Aleid J., and Jos P. Wubben. "Epidemiology of Botrytis cinerea Diseases in Greenhouses." In Botrytis: Biology, Pathology and Control. Springer Netherlands, 2007. http://dx.doi.org/10.1007/978-1-4020-2626-3_17.

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Van Der Cruyssen, G., and O. Kamoen. "Regulation of Polygalacturonases of Botrytis Cinerea." In Developments in Plant Pathology. Springer Netherlands, 1993. http://dx.doi.org/10.1007/978-94-011-1737-1_16.

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Schumacher, Julia, and Paul Tudzynski. "Morphogenesis and Infection in Botrytis cinerea." In Topics in Current Genetics. Springer Berlin Heidelberg, 2011. http://dx.doi.org/10.1007/978-3-642-22916-9_11.

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Elmer, Philip A. G., and Themis J. Michailides. "Epidemiology of Botrytis cinerea in Orchard and Vine Crops." In Botrytis: Biology, Pathology and Control. Springer Netherlands, 2007. http://dx.doi.org/10.1007/978-1-4020-2626-3_14.

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Lyon, Gary D., Bernard A. Goodman, and Brian Williamson. "Botrytis cinerea Perturbs Redox Processes as an Attack Strategy in Plants." In Botrytis: Biology, Pathology and Control. Springer Netherlands, 2007. http://dx.doi.org/10.1007/978-1-4020-2626-3_8.

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Atti di convegni sul tema "Botrytis cinerea"

Thakur, Suraj, Shubham, and Dhruv Chona. "Precision Disease Grading of Botrytis Cinerea in Kiwifruit Leaves Via CNNs and Logistic Regression." In 2024 4th International Conference on Technological Advancements in Computational Sciences (ICTACS). IEEE, 2024. https://doi.org/10.1109/ictacs62700.2024.10841261.

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Shkel, Daria, Justine Vanden Heuvel, Kerik Cox, Kirstin Petersen, and Amit Lal. "Portable Fungi Growth Detection of Botrytis Cinerea Enabled by Swept Frequency GHz Ultrasonic Imaging." In 2024 IEEE Ultrasonics, Ferroelectrics, and Frequency Control Joint Symposium (UFFC-JS). IEEE, 2024. https://doi.org/10.1109/uffc-js60046.2024.10794047.

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Saleh, Iman, and Mohammed Abu-Dieyeh. "Novel Prosopis Juliflora Leaf Ethanolic extract as natural Antifungal agent against Botrytis Cinerea: Application on Strawberries’ shelf-life extension." In Qatar University Annual Research Forum & Exhibition. Qatar University Press, 2021. http://dx.doi.org/10.29117/quarfe.2021.0044.

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Spoilage from fresh produces is a worldwide concern that accompanies the global increase in food demand. Adverse human health and environmental effects of commercial pesticides is a major public concern. Botrytis cinerea is one of the top ten pathogens that affect fresh produce including strawberries’ shelf-life around the world. Botrytis cinerea can progress easily from infected fruits to healthy ones even at low storage temperatures, which can lead to spoilage of entire lots in few weeks. Strawberries are widely consumed raw berries, which are famous in their processed forms such as jam and

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Rodas, Alejandro, Julio César Chavarro Porras, and Gloria Edith Guerrero Álvarez. "Modelo de recomendación para alerta temprana del hongo Botritys Cinerea en el cultivo de Mora de Castilla sin espinas (Rubus Glaucus Benth) en el departamento de Risaralda." In Nuevas realidades para la educación en ingeniería: currículo, tecnología, medio ambiente y desarrollo. Asociación Colombiana de Facultades de Ingeniería - ACOFI, 2022. http://dx.doi.org/10.26507/paper.2627.

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En la agricultura, el control de enfermedades se ha considerado como una tarea desafiante. Dentro de las especies que afectan el cultivo de Mora de Castilla se encuentra el hongo Botrytis cinerea, el cual puede generar pérdidas (entre el 50 y el 76 % del fruto cosechado). Generalmente, la opción empleada para combatirlo es a través de métodos químicos. Las tendencias actuales en la producción de alimentos están orientadas a obtener productos con menor carga de pesticidas, exigencias en conservación ambiental e inocuidad de los alimentos. Una ayuda para los agricultores es el desarrollo de herr

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Nijhawan, Rahul, Rahul Kumar Singh, Rajendra Singh Bisht, Neha Mendirtta, Pritish Dhir, and Vaishnavi Singh. "Detection of Botrytis Cinerea in Grapes using Machine Learning Technique." In 2023 International Conference on Evolutionary Algorithms and Soft Computing Techniques (EASCT). IEEE, 2023. http://dx.doi.org/10.1109/easct59475.2023.10393855.

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Liu, Yutong, Justin Kuo, Kerik Cox, Justine Vanden Heuvel, Kirstin Petersen, and Amit Lal. "Imaging and Detection of Botrytis Cinerea with Gigahertz Ultrasonic Imager." In 2021 IEEE International Ultrasonics Symposium (IUS). IEEE, 2021. http://dx.doi.org/10.1109/ius52206.2021.9593815.

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"Optimizing the Efficacy of Beneficial Bacteria Against Botrytis Blight Disease in Petunias." In Plant Health 2024. American Phytopathological Society, 2024. http://dx.doi.org/10.1094/aps-ph24-023.

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Botrytis cinerea, a fungal pathogen and the causal agent of botrytis blight or gray mold, poses a significant challenge in greenhouse crop management due to its wide host range and ability to infect plants at various growth stages. The reliance on conventional fungicides has led to environmental concerns and the emergence of fungicide-resistant strains. This study explores the use of beneficial Pseudomonas bacteria, hypothesizing that specific strain combinations could suppress B. cinerea more effectively than single strains. We focused on five Pseudomonas strains (14B11, AP54, 15H3, 94G2, 89F

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Jia, Li-yuan, and Xin-she Liu. "Effect of Six Fungicides against Botrytis Cinerea on Protected Cultivation Tomato." In 2011 Second International Conference on Digital Manufacturing and Automation (ICDMA). IEEE, 2011. http://dx.doi.org/10.1109/icdma.2011.124.

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Leandro Rodrigues, Rita Alaide, Enio Nazaré de Oliveira Junior, and Thamara Carvalho Coutinho. "CONTROLE DO CRESCIMENTO DO FUNGO BOTRYTIS CINEREA EM MORANGOS UTILIZANDO QUITOSANA." In Simpósio Nacional de Bioprocessos e Simpósio de Hidrólise Enzimática de Biomassa. Galoá, 2015. http://dx.doi.org/10.17648/sinaferm-2015-33436.

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Rodas, Alejandro, Julio César Chavarro Porras, and Gloria Edith Guerrero Álvarez. "Desarrollo de plataforma de bajo costo para el monitoreo de condiciones microclimáticas favorables al surgimiento del hongo Botrytis Cinerea en el cultivo de mora de Castilla departamento Risaralda." In Ingeniería: una transición hacia el futuro. Asociación Colombiana de Facultades de Ingeniería - ACOFI, 2024. http://dx.doi.org/10.26507/paper.3521.

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La mora de Castilla (Rubus glaucus Benth) se caracteriza por crecer en climas fríos y moderados en países de la región andina y es considerado un cultivo importante en diferentes zonas de Colombia. La mora de Castilla es afectada por el hongo Botrytis cinerea, este es favorecido por factores ambientales como bajas temperaturas, alta humedad relativa, altas precipitaciones, humedad de la hoja y condiciones de luz (espectro UV) las cuales influyen en cada una de etapas de desarrollo de la planta bajo una amplia gama de condiciones de microclimáticas. Por tanto, es primordial realizar estudios en

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Rapporti di organizzazioni sul tema "Botrytis cinerea"

Sharon, Amir, and Tesfaye Mengiste. Molecular dissection of host and pathogen factors in Botrytis cinerea pathogenesis for improved genetic resistance. United States Department of Agriculture, 2016. http://dx.doi.org/10.32747/2016.7604272.bard.

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Sharon, Amir, and Maor Bar-Peled. Identification of new glycan metabolic pathways in the fungal pathogen Botrytis cinerea and their role in fungus-plant interactions. United States Department of Agriculture, 2012. http://dx.doi.org/10.32747/2012.7597916.bard.

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Abstract (sommario):

The involvement of glycans in microbial adherence, recognition and signaling is often a critical determinant of pathogenesis. Although the major glycan components of fungal cell walls have been identified there is limited information available on its ‘minor sugar components’ and how these change during different stages of fungal development. Our aim was to define the role of Rhacontaining-glycans in the gray mold disease caused by the necrotrophic fungus B. cinerea. The research was built on the discovery of two genes, Bcdhand bcer, that are involved in formation of UDP-KDG and UDP-Rha, two UD

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Lichter, Amnon, Joseph L. Smilanick, Dennis A. Margosan, and Susan Lurie. Ethanol for postharvest decay control of table grapes: application and mode of action. United States Department of Agriculture, 2005. http://dx.doi.org/10.32747/2005.7587217.bard.

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Original objectives: Dipping of table grapes in ethanol was determined to be an effective measure to control postharvest gray mold infection caused by Botrytis cinerea. Our objectives were to study the effects of ethanol on B.cinerea and table grapes and to conduct research that will facilitate the implementation of this treatment. Background: Botrytis cinerea is known as the major pathogen of table grapes in cold storage. To date, the only commercial technology to control it relied on sulfur dioxide (SO₂) implemented by either fumigation of storage facilities or from slow release generator pa

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Reisch, Bruce, Avichai Perl, Julie Kikkert, Ruth Ben-Arie, and Rachel Gollop. Use of Anti-Fungal Gene Synergisms for Improved Foliar and Fruit Disease Tolerance in Transgenic Grapes. United States Department of Agriculture, 2002. http://dx.doi.org/10.32747/2002.7575292.bard.

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Original objectives . 1. Test anti-fungal gene products for activity against Uncinula necator, Aspergillus niger, Rhizopus stolonifer and Botrytis cinerea. 2. For Agrobacterium transformation, design appropriate vectors with gene combinations. 3. Use biolistic bombardment and Agrobacterium for transformation of important cultivars. 4. Characterize gene expression in transformants, as well as level of powdery mildew and Botrytis resistance in foliage of transformed plants. Background The production of new grape cultivars by conventional breeding is a complex and time-consuming process. Transfer

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Lichter, Amnon, Gopi K. Podila, and Maria R. Davis. Identification of Genetic Determinants that Facilitate Development of B. cinerea at Low Temperature and its Postharvest Pathogenicity. United States Department of Agriculture, 2011. http://dx.doi.org/10.32747/2011.7592641.bard.

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Botrytis cinerea is the postharvest pathogen of many agricultural produce with table grapes, strawberries and tomatoes as major targets. The high efficiency with which B. cinerea causes disease on these produce during storage is attributed in part due to its exceptional ability to develop at very low temperature. Our major goal was to understand the genetic determinants which enable it to develop at low temperature. The specific research objectives were: 1. Identify expression pattern of genes in a coldenriched cDNA library. 2. Identify B. cinerea orthologs of cold-induced genes 3. Profile pro

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Chalutz, Edo, Michael Wisniewski, Samir Droby, Yael Eilam, and Ilan Chet. Mode of Action of Yeast Biocontrol Agents of Postharvest Diseases of Fruits. United States Department of Agriculture, 1996. http://dx.doi.org/10.32747/1996.7613025.bard.

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In a previous BARD-supported study, three of the investigators of this research were involved in a study on biological control of postharvest diseases of citrus and deciduous fruits. Several naturally occurring, non-antibiotic producing yeast antagonists were identified. Application of some of these antagonists resulted in very high levels of biocontrol under laboratory conditions but lower efficacy in semi-commercial tests. It was felt that the lack of knowledge on the mode of action of the biocontrol agents was limiting their efficient use. The current study was aimed at narrowing this gap i

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Granot, David, Richard Amasino, and Avner Silber. Mutual effects of hexose phosphorylation enzymes and phosphorous on plant development. United States Department of Agriculture, 2006. http://dx.doi.org/10.32747/2006.7587223.bard.

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Research objectives 1) Analyze the combined effects of hexose phosphorylation and P level in tomato and Arabidopsis plants 2) Analyze the combined effects of hexose phosphorylation and P level in pho1 and pho2 Arabidopsis mutants 3) Clone and analyze the PHO2 gene 4) Select Arabidopsis mutants resistant to high and low P 5) Analyze the Arabidopsis mutants and clone the corresponding genes 6) Survey wild tomato species for growth characteristics at various P levels Background to the topic Hexose phosphorylating enzymes, the first enzymes of sugar metabolism, regulate key processes in plants suc

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Dickman, Martin B., and Oded Yarden. Modulation of the Redox Climate and Phosphatase Signaling in a Necrotroph: an Axis for Inter- and Intra-cellular Communication that Regulates Development and Pathogenicity. United States Department of Agriculture, 2011. http://dx.doi.org/10.32747/2011.7697112.bard.

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The long-term goals of our research are to understand the regulation of sclerotial development and pathogenicity in S. sclerotiorum. The focus in this project is on the elucidation of the signaling events and environmental cues that contribute to broad pathogenic success of S. sclerotiorum. In this proposal, we have taken advantage of the recent conceptual (ROS/PPs signaling) and technical (genome sequence availability and gene inactivation possibilities) developments to address the following questions, as appear in our research goals stated below, specifically concerning the involvement of RE

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Droby, Samir, Michael Wisniewski, Ron Porat, and Dumitru Macarisin. Role of Reactive Oxygen Species (ROS) in Tritrophic Interactions in Postharvest Biocontrol Systems. United States Department of Agriculture, 2012. http://dx.doi.org/10.32747/2012.7594390.bard.

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To elucidate the role of ROS in the tri-trophic interactions in postharvest biocontrol systems a detailed molecular and biochemical investigation was undertaken. The application of the yeast biocontrol agent Metschnikowia fructicola, microarray analysis was performed on grapefruit surface wounds using an Affymetrix Citrus GeneChip. the data indicated that 1007 putative unigenes showed significant expression changes following wounding and yeast application relative to wounded controls. The expression of the genes encoding Respiratory burst oxidase (Rbo), mitogen-activated protein kinase (MAPK)

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Watad, Abed A., Paul Michael Hasegawa, Ray A. Bressan, Alexander Vainstein, and Yigal Elad. Osmotin and Osmotin-Like Proteins as a Novel Source for Phytopathogenic Fungal Resistance in Transgenic Carnation and Tomato Plants. United States Department of Agriculture, 2000. http://dx.doi.org/10.32747/2000.7573992.bard.

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The goal of this project is to enhance fungal resistance of carnation and tomato through the ectopic expression of osmotin and other pathogenesis-related (PR) proteins. The research objectives were to evaluate in vitro antifungal activity of osmotin and osmotin and other PR protein combinations against phytopathogens (including Fusarium oxysporum, Verticillium dahliae, Botrytus cinerea or Phytophthora infestans), develop protocols for efficient transformation of carnation and tomato, express PR proteins in transgenic carnation and tomato and evaluate fungal resistance of transgenic plants. Pro

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