Tesi sul tema "Chlamydomonas reinhardtii – Genetics"
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Goho, Shaun. "The accumulation of variance in fitness in clonal populations of Chlamydomonas reinhardtii in normal and stressful environments /". Thesis, McGill University, 1997. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=27328.
The second chapter extends the investigation from normal culturing conditions into stressful ones. Specifically, it considers the hypothesis that C. reinhardtii might increase its mutation rate as a general response to environmental stress. Stressed lines were found to display reduced mean fitness and an increased variance of fitness after being returned to normal culturing conditions. This was interpreted as evidence for increased mutation rates in treated lines relative to controls. Possible mechanisms underlying this phenomenon are discussed, along with suggestions for further research.
Chao, Vincent 1973. "Ecological and sexual divergence in experimental populations of Chlamydomonas". Thesis, McGill University, 2001. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=32982.
Stevens, David Roy. "Nuclear transformation and gene expression in Chlamydomonas reinhardtii". Thesis, University College London (University of London), 1996. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.362931.
Wong, Ka-ho, e 王家豪. "Transgenic chlamydomonas reinhardtii as an experimental system to study the regulation of carotenoid biosynthesis in green microalgae". Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 2006. http://hub.hku.hk/bib/B37728337.
Hessenberger, Daisy Sophia Innes. "Small RNA and genome interactions in Chlamydomonas reinhardtii recombinants". Thesis, University of Cambridge, 2015. https://www.repository.cam.ac.uk/handle/1810/274914.
Feldman, Jessica L. "Deconstructing cell architecture: Exploring centriole structure, function, and position in the green alga Chlamydomonas reinhardtii". Diss., Search in ProQuest Dissertations & Theses. UC Only, 2008. http://gateway.proquest.com/openurl?url_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:dissertation&res_dat=xri:pqdiss&rft_dat=xri:pqdiss:3324579.
Zeyl, Clifford. "Sex, parasitic DNA and adaptation in experimental populations of Saccharomyces cerevisiae and Chlamydomonas reinhardtii". Thesis, McGill University, 1996. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=40475.
Gaskill, Christa. "Towards an Action Spectrum for Photoentrainment of the Chlamydomonas ReinhardtII Circadian Clock". TopSCHOLAR®, 2008. http://digitalcommons.wku.edu/theses/43.
Castonguay, Andrew David. "Analysis of mutants impaired for respiratory growth in the model photosynthetic alga, Chlamydomonas reinhardtii". The Ohio State University, 2021. http://rave.ohiolink.edu/etdc/view?acc_num=osu1619140884575211.
Barbieri, Maria del Rosario. "The green alga Chlamydomonas reinhardtii: a new model system to unravel the biogenesis of respiratory complexes". The Ohio State University, 2010. http://rave.ohiolink.edu/etdc/view?acc_num=osu1271966493.
Silparasetty, Shobha Lavanya. "Cloning of "Animal Cryptochrome" cDNA from the Model Organism CHLAMYDOMONAS REINHARDTII for Functional Analysis of Its Protein Product". TopSCHOLAR®, 2009. http://digitalcommons.wku.edu/theses/117.
Amos, B. Kirtley. "Up Regulation of Heat Shock Protein 70B (HSP70B) and SSA1 in Chlamydomonas Reinhardtii via HSP70A-RBCS2 and PSAD Promoter". UKnowledge, 2015. http://uknowledge.uky.edu/bae_etds/39.
Haji, Taha Hussein. "Genetic manipulation of fermentative metabolism in Chlamydomonas reinhardtii". Thesis, Imperial College London, 2015. http://hdl.handle.net/10044/1/38619.
Ray, Nicola. "Genetic manipulation of photosystem two polypeptides in Chlamydomonas reinhardtii". Thesis, University College London (University of London), 2001. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.395045.
Fong, Nga Yin. "The trehalose metabolism in different stress responses of chlamydomonas reinhardtii /". View abstract or full-text, 2005. http://library.ust.hk/cgi/db/thesis.pl?BIOL%202005%20FONG.
Thompson, Craig Peter. "Genetic analysis of RNA silencing in the unicellular alga Chlamydomonas reinhardtii". Thesis, University of Cambridge, 2013. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.607961.
Ninlayarn, T. "Chloroplast genetic engineering in the microalga Chlamydomonas reinhardtii : molecular tools and applications". Thesis, University College London (University of London), 2012. http://discovery.ucl.ac.uk/1344073/.
Berger, Christopher Michael. "The genetic basis of cooperative aggregation in the green alga Chlamydomonas reinhardtii". Thesis, Kansas State University, 2017. http://hdl.handle.net/2097/35472.
Division of Biology
Bradley J. Olson
Unicellular organisms alter their behavior and morphology in response to environmental stresses, particularly in response to immediate threats to their survival. A common tactic of predator avoidance for unicellular green algae is to aggregate to form groups. We have found that the model unicellular green algae Chlamydomonas reinhardtii forms aggregates in response to the presence of the filter feeding zooplanktonic predator, Daphnia magna. Chalmydomonas is a member of the volvocine algae, a morphologically diverse group of closely related green algae that is often used to study multicellular development. We have characterized aggregation in Chlamydomonas reinhardtii and found that it is rapid, transient and induced by signals originating from the Daphnia predators. To understand the genetic basis of cooperative aggregation we used an RNA-seq approach. RNA-seq characterized the transcriptomic response by Chlamydomonas during aggregation, and we identified 131 genes are significantly differentially expressed between predated and unpredated cultures of Chlamydomonas. Several candidate genes were characterized based on existing annotations, evolutionary history and expression profile. Evolutionary relationships between candidate aggregation genes in Chlamydomonas and their orthologs in multicellular Volvocales suggest a possible role of aggregation genes in multicellular development. Our results demonstrate that Chlamydomonas dynamically alters its morphology based on its environment and identify several candidate genes for aggregation and multicellular development.
Malnoë, Alizée. "A genetic suppressor approach to the biogenesis, quality control and function of photosynthetic complexes in Chlamydomonas reinhardtii". Phd thesis, Université Paris Sud - Paris XI, 2011. http://tel.archives-ouvertes.fr/tel-01057821.
Bölling, Christian. "Comprehensive metabolite analysis in Chlamydomonas reinhardtii : method development and application to the study of environmental and genetic perturbations". Phd thesis, Universität Potsdam, 2006. http://opus.kobv.de/ubp/volltexte/2006/1132/.
Entwicklung und Anwendung von Methoden zur multiparallelen Analyse von Metaboliten in der einzelligen Grünalge Chlamydomonas reinhardtii, einem der wichtigsten Modellorganismen der Zellbiologie, sind Gegenstand dieser Arbeit. Metabolomanalyse, die umfassende Analyse von Veränderungen der Konzentrationen von Stoffwechselprodukten durch Umweltreize oder genetische und entwicklungsbedingte Signale, ist ein wichtiges Komplement anderer Genomanalysemethoden, um die Integration von Genen, Proteinen und Metaboliten in ein nahtloses und dynamisches Netzwerk zur Aufrechterhaltung der Lebensfunktionen eines Organismus zu verstehen. Die Methode wurde im Hinblick auf schnelle Inaktivierung enzymatischer Aktivität, Maximierung der Extraktionskapazität und Behandlung großer Probenmengen optimiert. Im Ergebnis der Probenaufarbeitung, Extraktion und Analyse mittels Gaschromatographie und Time-Of-Flight-Massenspektrometrie konnten mehr als 800 analytische Signale in Einzelproben dargestellt werden, von denen über 100 identifiziert werden konnten. Die Arbeit stellt methodische Innovationen zur systematischen Erkennung von Artefakten in GC-MS Chromatogrammen und Werkzeuge zur Anwendung der Hauptkomponentenanalyse auf Metabolom-Daten vor. Zellen unter Stickstoff- (N), Phosphor- (P), Schwefel- (S), oder Eisen- (Fe) Mangel zeigen deutliche Unterschiede in ihrer Metabolitenausstattung. Die Anpassung an die einzelnen Nährstoffmangelsituationen ist durch spezifische Änderungen einer Reihe von Metaboliten zentraler Prozesse des Primärstoffwechsels gekennzeichnet. Die Konzentrationsänderungen von Substraten für die Stickstoffassimilation und den oxidativen Pentosephosphatweg deuten darauf hin, dass die Fähigkeit zur schnellen Aktivierung der N-Assimilation auch unter Bedingungen herabgesetzter Stoffwechsel- und Wachstumsaktivität aufrechterhalten wird. Die Akkumulation von 4-Hydroxyprolin unter Schwefelmangel könnte im Zusammenhang stehen mit der Degradation von Proteinen der Chlamydomonas-Zellwand, deren wesentlicher Bestandteil hydroxyprolinreiche Glykoproteine sind und die unter Schwefelmangel aktiv umgebaut wird. Die Anpassung an Schwefelmangel wurde mit größerer zeitlicher Auflösung in Wildtyp-Zellen und Zellen des sac1-Stammes untersucht. SAC1 ist ein zentraler Regulator der Schwefelmangelantwort in Chlamydomonas. Zeitgleiche Ab- und Zunahme von Metaboliten ist ein charakteristisches Element der Anpassung an Schwefelmangel in Wildtypzellen. Die Reaktion von SAC1-Mutanten auf Schwefelmangel ist durch weit reichenden Verlust zur Steuerung von Prozessen gekennzeichnet, die normalerweise zur vorübergehenden oder dauerhaften Anreicherung bestimmter Metabolite führen. Die Verfügbarkeit von Chlamydomonas-Stämmen mit fehlender Enzymaktivität für fast jeden der Schritte der Argininbiosynthese eröffnet die Möglichkeit, das Potential der Metabolitenanalyse zur Untersuchung der Regulation der Aminosäurebiosynthese in photosynthetischen Eukaryoten zur Anwendung zu bringen. Drei Stämme, mit fehlender Aktivität für N-Acetylglutamat-5-phosphat Reduktase (arg1), N2 Acetylornithin-Aminotransferase (arg9) beziehungsweise Argininosuccinat Lyase (arg2) wurden in Bezug auf die Aktivierung ihrer endogenen Argininbiosynthese nach Entzug externer Argininquellen analysiert. Die einzelnen enzymatischen Blocks konnten durch Precursor-Anreicherung, wie die Anhäufung von Argininosuccinat in arg2-Zellen, und Erschöpfung von Intermediaten nachgelagerter Reaktionen, beispielsweise die deutliche Abnahme von N2-Acetylornithin, Ornithin und Argininosuccinat in arg9-Zellen charakterisiert werden. Das unerwartete Vorhandensein von zum Teil das Wildtyp-Niveau überschreitender Mengen von N2-Acetylornithin, Citrullin und Argininosuccinat, die Produkte bzw. Substrate dem enzymatischen Block nachgelagerter Reaktionen in arg1-Zellen sind, bot eine Erklärung für eine noch vorhandene Restkapazität zum Wachstum des arg1-Stamms auch ohne äußere Arginingabe. Der Nachweis dieser Verbindungen sowie die ungewöhnliche Anreicherung von N-Acetylglutamat, der ersten Verbindung, die das Glutamat-Gerüst für die Ornithin- und Argininsynthese bindet, in arg1-Zellen könnte auf alternative Reaktionen, möglicherweise unter Beteiligung von Ornithin-Aminotransferase, zur Synthese von Ornithin hindeuten, die in Erscheinung treten, wenn die Synthesekette nach Acetylierung von Glutamat blockiert ist.
Villela, Helena Dias Müller. "Utilização das técnicas de engenharia genética e bioquímica em Chlamydomonas reinhardtii visando o aumento da produção de lipídeos para obtenção de biocombustível". Universidade de São Paulo, 2014. http://www.teses.usp.br/teses/disponiveis/46/46131/tde-01102014-080611/.
The environmental impacts caused by gases emitted from burning fossil fuels and their manipulation, combined with rising fuel prices, has stimulated demand for new renewable resources and developing new green technologies that support the industry and market needs. Biofuels are biodegradable and renewable resources, which come out to be an economically viable alternative. However, the current generation of biofuels has some disadvantages, such as: use of fertile soils and competition with the food industry, once it uses crops such as soybeans, corn and sugar cane, products of extreme economic importance to the producing countries. For these reasons, there is a growing interest in exploring other possible raw materials, especially those that are geared exclusively for power generation. In this context, microalgae have shown to be a very interesting option. These organisms have a high potential because they have fast growth rate and the ability to produce large amounts of oil. In addition, biofuel production by these organisms can be optimized for both the modification of culture conditions (biochemical engineering), and through the genetic manipulation of microalgae strains (genetic engineering). In this work, the two strategies have been used in order to increase the amount of lipid produced by the strain CC424 from the model organism Chlamydomonas reinhardtii. The metabolic route chosen for genetic manipulation is the glyoxylate cycle, and the two key enzymes of this cycle, isocitrate lyase (icl) and malate synthase (ms), the targets. The plasmid pSL18 was used as a vector of transformation in the microalgae. Six types of transformant strains were obtained, two of them overexpressing the ms and icl genes separately, two underexpressing these genes and two double transformations, one of them overexpressing both genes at the same time the other one underexpressing them. The strain underexpressing both enzymes at the same time, showed a significant increase in the amount of neutral lipids. In this mutant, the shortage of nitrogen led to an even greater increase in these lipids. While in normal media the difference between the amount of lipids was 1.5 times, under nitrogen starvation the difference was approximately 3 times, corroborated by the difference in gene expression levels, which was also about 3 times. Moreover, the mutant strain also showed an increase in each of the individual fatty acids analyzed, revealing a large amount in all kinds of C16 and C18 fatty acids, important for biodiesel that suits the regulation of Agência Nacional de Petróleo, Gás Natural e Biocombustíveis. Although the mutant Dupla-ICL-MS-anti produces higher amounts of lipids compared to the wild type, the strain showed no critical negative effects. Both the production of biomass and the amount of chlorophylla, total protein and total carbohydrates remained stable after the introduction of the mutation. These results suggest that the enzymes of the glyoxylate cycle, which are linked to the catabolism of fatty acids, can be used as promising targets for the optimization of strains already used commercially in the production of biodiesel.
Bölling, Christian. "Comprehensive metabolite analysis in Chlamydomonas reinhardtii method development and application to the study of environmental and genetic perturbations /". [S.l.] : [s.n.], 2006. http://opus.kobv.de/ubp/volltexte/2006/1132.
Kelterborn, Simon. "Gen-Editierung von Photorezeptorgenen in der Grünalge Chlamydomonas reinhardtii mithilfe des CRISPR/Cas9-Systems". Doctoral thesis, Humboldt-Universität zu Berlin, 2020. http://dx.doi.org/10.18452/21903.
Gene editing is a fundamental tool in molecular biosciences in order to study the function of genes (reverse genetics). This study established zinc-finger and CRISPR/Cas9 nucleases for gene editing to target and inactivate the photoreceptor genes in C. reinhardtii. In continuation of previous work with designer zinc-finger nucleases (ZFN), the transformation efficiency could be improved 300-fold, which enabled the inactivation of genes in motile wild type cells. This made it possible to disrupt the Channelrhodopsin-1 (ChR1), Channelrhodopsin-2 (ChR2) and Chlamyopsin-1/2 (COP1/2) genes individually and in parallel. Phototaxis experiments in these strains revealed that the inactivation of ChR1 had a greater effect on phototaxis than the inactivation of ChR2. To apply the CRISPR/Cas9 system, the transformation conditions were adapted and optimized so that the Cas9-gRNA complex was successfully electroporated into the cells as an in vitro synthesized ribonucleoprotein. This approach enabled gene inactivations with CRISPR/Cas9 in C. reinhardtii. In order to measure and improve the conditions for precise gene modifications, the SNRK2.2 gene was established as a reporter gene for a ‘Blue-Green test’. Small insertions of up to 30 bp were inserted using short oligonucleotides, while larger reporter genes (mVenus, SNAP-tag) were integrated using donor plasmids. Throughout this study, more than 20 non-selectable genes were disrupted, including 10 of the photoreceptor genes, with an average mutation rate of 12,1 %. Overall, this work shows in a comprehensive way how gene inactivations and modifications can be performed in green alga C. reinhardtii using ZFNs or CRISPR/Cas9. In addition, the collection of the ten photoreceptor knockouts provides a promising source to investigate the diversity of photoreceptor genes in C. reinhardtii.
Wirschell, Maureen. "Chlamydomonas Reinhardtii ODA5 Encodes an Axonemal Protein Required for Assembly of the Outer Dynein Arm and an Associated Flagellar Adenylate Kinase: A Dissertation". eScholarship@UMMS, 2004. https://escholarship.umassmed.edu/gsbs_diss/25.
Lee, Jaesung. "Mutagenesis and suppression of a light-regulated group I intron in the chloroplast psbA gene of Chlamydomonas reinhardtii". Thesis, 2003. http://wwwlib.umi.com/cr/utexas/fullcit?p3118038.
Wu, Li-Ping. "Mutational and biochemical analysis of the cell cycle in Chlamydomonas reinhardtii". Phd thesis, 1993. http://hdl.handle.net/1885/142311.
Sakuanrungsirikul, Suchirat. "Cyclic AMP and the Chlamydomonas reinhardtii cell division cycle". Phd thesis, 1991. http://hdl.handle.net/1885/142214.
Heinrich, Oliver. "Das Argininosuccinat Lyase-Gen von Volvox carteri : Struktur und heterologe Expression in Chlamydomonas reinhardtii /". 2000. http://www.gbv.de/dms/bs/toc/321061195.pdf.
Bölling, Christian [Verfasser]. "Comprehensive metabolite analysis in Chlamydomonas reinhardtii : method development and application to the study of environmental and genetic perturbations / von Christian Bölling". 2006. http://d-nb.info/982939027/34.