Bibliografie tematiche / Digital droplet PCR

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Letteratura scientifica selezionata sul tema "Digital droplet PCR"

Autore: Grafiati
Pubblicato: 4 giugno 2021
Ultima modifica: 25 luglio 2025

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Articoli di riviste sul tema "Digital droplet PCR"

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Zhou, Wuping, Cong Liu, Tao Zhang, et al. "Low Cost, Easily-Assembled Centrifugal Buoyancy-Based Emulsification and Digital PCR." Micromachines 13, no. 2 (2022): 171. http://dx.doi.org/10.3390/mi13020171.

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Microfluidic-based droplet generation approaches require the design of microfluidic chips and a precise lithography process, which require skilled technicians and a long manufacturing time. Here we developed a centrifugal buoyancy-based emulsification (CBbE) method for producing droplets with high efficiency and minimal fabrication time. Our approach is to fabricate a droplet generation module that can be easily assembled using syringe needles and PCR tubes. With this module and a common centrifuge, high-throughput droplet generation with controllable droplet size could be realized in a few mi
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Schuler, Friedrich, Martin Trotter, Marcel Geltman, et al. "Digital droplet PCR on disk." Lab on a Chip 16, no. 1 (2016): 208–16. http://dx.doi.org/10.1039/c5lc01068c.

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Meng, Xiangkai, Yuanhua Yu, and Guangyong Jin. "Numerical Simulation and Experimental Verification of Droplet Generation in Microfluidic Digital PCR Chip." Micromachines 12, no. 4 (2021): 409. http://dx.doi.org/10.3390/mi12040409.

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The generation of droplets is one of the most critical steps in the droplet digital polymerase chain reaction (ddPCR) procedure. In this study, the mechanism of droplet formation in microchannel structure and factors affecting droplet formation were studied. The physical field of laminar two-phase flow level was used to simulate the process of droplet generation through microfluidic technology. The effect of the parameters including flow rate, surface tension, and viscosity on the generated droplet size were evaluated by the simulation. After that, the microfluidic chip that has the same dimen
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Fitarelli-Kiehl, Mariana, Fangyan Yu, Ravina Ashtaputre, et al. "Denaturation-Enhanced Droplet Digital PCR for Liquid Biopsies." Clinical Chemistry 64, no. 12 (2018): 1762–71. http://dx.doi.org/10.1373/clinchem.2018.293845.

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Abstract BACKGROUND Although interest in droplet-digital PCR technology (ddPCR) for cell-free circulating DNA (cfDNA) analysis is burgeoning, the technology is compromised by subsampling errors and the few clinical targets that can be analyzed from limited input DNA. The paucity of starting material acts as a “glass ceiling” in liquid biopsies because, irrespective how analytically sensitive ddPCR techniques are, detection limits cannot be improved past DNA input limitations. METHODS We applied denaturation-enhanced ddPCR (dddPCR) using fragmented genomic DNA (gDNA) with defined mutations. We
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Nugroho, Kristianto, Dwi Widyajayantie, Sayyidah Afridatul Ishthifaiyyah, and Elisa Apriliani. "Pemanfaatan Teknologi Droplet Digital PCR (ddPCR) dalam Kegiatan Analisis Molekuler Tanaman." JURNAL BIOS LOGOS 11, no. 1 (2021): 28. http://dx.doi.org/10.35799/jbl.11.1.2021.31101.

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(Article History: Received 23 October 2020; Revised 9 January 2021; Accepted 18 January 2021) ABSTRAKSelama beberapa dekade terakhir, teknik PCR memberikan manfaat yang begitu besar dalam kegiatan penelitian di bidang biologi molekuler. Digital droplet PCR (ddPCR) merupakan salah satu teknologi PCR terbaru yang diklaim memiliki keunggulan dibanding teknik qPCR. Prinsip kerja teknik ini yaitu membagi sampel menjadi molekul-molekul kecil yang dipisahkan oleh emulsi minyak, air, dan senyawa penstabil sehingga membentuk droplets. Teknik ini memiliki kelebihan mampu melakukan kuantifikasi absolut m
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Dutra, Lara, Ole Franz, Veli-Mikko Puupponen, and Marja Tiirola. "DNA recovery from Droplet Digital™ PCR emulsions using liquid nitrogen." BioTechniques 69, no. 6 (2020): 450–54. http://dx.doi.org/10.2144/btn-2020-0076.

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Droplet microfluidics is a technology that enables the production and manipulation of small volumes. In biosciences, the most popular application of this technology is Droplet Digital™ PCR (ddPCR™), where parallel nanoliter-scale PCR assays are used to provide a high sensitivity and specificity for DNA detection. However, the recovery of PCR products for downstream applications such as sequencing can be challenging due to the droplets' stability. Here we compared five methods for disrupting the droplets to recover DNA. We found that rapid freezing in liquid nitrogen results in a clear phase se
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Luo, Gangyin, Ying Zhang, Shun Wang, Xinbei Lv, Tianhang Yang, and Jinxian Wang. "Establishment and Validation of an Integrated Microfluidic Step Emulsification Chip Supporting Droplet Digital Nucleic Acid Analysis." Biosensors 13, no. 9 (2023): 888. http://dx.doi.org/10.3390/bios13090888.

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Uniform and stable droplet generation is critical for accurate and efficient digital nucleic acid analysis (dNAA). In this study, an integrated microfluidic step emulsification device with wide-range droplet generation capability, small device dimensions, convenient fabrication strategy, low contamination and high robustness was developed. A tree-shaped droplet generation nozzle distribution design was proposed to increase the uniformity of droplet generation by equating flow rates, and the flow field in the design was numerically simulated. Theoretical analysis and comparative experiments on
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Rausch, Christian, Maja Rothenberg-Thurley, Simon A. Buerger, et al. "Double Drop-Off Droplet Digital PCR." Journal of Molecular Diagnostics 23, no. 8 (2021): 975–85. http://dx.doi.org/10.1016/j.jmoldx.2021.05.001.

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Lo Schirico, Mariella, Martina Ferrante, Irene Dogliotti, et al. "Droplet Digital PCR Assay for MYD88L265P." HemaSphere 4, no. 1 (2020): e324. http://dx.doi.org/10.1097/hs9.0000000000000324.

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Moser, Dirk A., Luca Braga, Andrea Raso, Serena Zacchigna, Mauro Giacca, and Perikles Simon. "Transgene Detection by Digital Droplet PCR." PLoS ONE 9, no. 11 (2014): e111781. http://dx.doi.org/10.1371/journal.pone.0111781.

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Tesi sul tema "Digital droplet PCR"

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Attali, Dean. "Automatic analysis of dual-channel Droplet Digital PCR experiments." Thesis, University of British Columbia, 2016. http://hdl.handle.net/2429/57928.

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The ability to quantify the amount of DNA in a sample is an essential technique in biology and is used in many fields of research. Droplet digital polymerase chain reaction (ddPCR) is an advanced technology developed for this purpose that enables more accurate and sensitive quantification than traditional real-time PCR. In ddPCR, nucleic acid (e.g., genomic DNA) within a sample is partitioned into thousands of droplets, along with the reagents needed to amplify and detect one or more DNA target sequences. After amplification takes place in all droplets, each droplet is individually read by a t
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Jakobsson, Sanna. "Quantitative analysis of BCR-ABL1 fusion gene by Droplet Digital PCR and qRT-PCR." Thesis, Umeå universitet, Biomedicinsk laboratorievetenskap, 2015. http://urn.kb.se/resolve?urn=urn:nbn:se:umu:diva-103957.

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Pettersson, Fredrika. "Identifiering och kvantifiering av humant papillomvirus typ 16 med droplet digital PCR." Thesis, Örebro universitet, Institutionen för hälsovetenskap och medicin, 2015. http://urn.kb.se/resolve?urn=urn:nbn:se:oru:diva-44983.

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Gautier, Célia. "Variation de l’expression génique au cours de l’hibernation du hamster d’Europe : un rôle des récepteurs à la mélatonine ?" Thesis, Strasbourg, 2018. http://www.theses.fr/2018STRAJ011/document.

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Afin de faire face aux conditions environnementales défavorables, certains animaux réduisent drastiquement leur activité métabolique et leur température grâce à des phases de torpeur hivernal. L’objectif de cette étude est d’établir une signature moléculaire de chacune des phases d’hibernation. Pour cela, les variations d’expression de 21 gènes impliqués dans le contrôle des fonctions saisonnières (horloge circadienne, hormones thyroïdiennes, récepteurs à la mélatonine) et le métabolisme ont été étudiées dans 8 organes. Les résultats ont mis en évidence une augmentation ubiquitaire de l’expres
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Brugière, Charlotte. "L'invasion péri-nerveuse des carcinomes épidermoïdes cutanés humains." Thesis, Sorbonne Paris Cité, 2018. http://www.theses.fr/2018USPCC193.

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Le carcinome épidermoïde cutané (CEC) représente un enjeu important par sa fréquence et sa gravité potentielle.L’agressivité de ce cancer est liée à l’invasion péri-nerveuse (IPN), mode d’envahissement tumoral reconnu comme un facteur de mauvais pronostic.L’objectif de ce travail est de s’intéresser aux mécanismes favorisant l’IPN, en comparant 2 groupes appariés de CEC humains, avec et sans IPN.Pour cela nous avons réalisé une étude de facteurs et récepteurs neurotrophiques, de marqueurs de la transition épithélio-mésenchymateuse (TEM), et de la molécule NCAM1, par analyse immunohistochimique
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Pekin, Deniz. "Development of novel droplet-based microfluidic strategies for the molecular diagnosis of cancer." Phd thesis, Université de Strasbourg, 2013. http://tel.archives-ouvertes.fr/tel-00856594.

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The aim of this work is to establish novel strategies for the highly sensitive screening of cancer biomarkers in biological samples.To achieve this goal, we developed droplet-based microfluidic dPCR technique. Using a limiting dilution, individual DNA molecules are encapsulated within monodisperse droplets of a water-in-oil emulsion created with a microfluidic device. Fluorescent TaqMan® probes targeting the screened cancer biomarkers allow the detection of mutations. We focused on the mutations in the human KRAS gene for the development of our test. This method is also transposable in a multi
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Wågberg, Johanna. "Detection of hotspot mutations in IDH1/2 in patients withacute myeloid leukemia using Droplet Digital PCR." Thesis, Örebro universitet, Institutionen för medicinska vetenskaper, 2020. http://urn.kb.se/resolve?urn=urn:nbn:se:oru:diva-81559.

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IntroductionAcute myeloid leukemia (AML) is caused by a wide range of genetic aberrations, includingmutations within the genes that encode the enzymes isocitrate dehydrogenase 1 and 2(IDH1/2). Drugs that target mutant IDH1/2 are now available, which makes assessment of themutational status of IDH1/2 important in clinical diagnostics of AML. A promising method todetect these mutations is the droplet digital polymerase chain reaction (ddPCR), which showsadvantages of a high sensitivity and a simple workflow.AimTo evaluate ddPCR as method of choice to detect hotspot mutations in IDH1 (codon R132)
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Ziller, Antoine. "Origine(s) et Fonction(s) de Gènes de Résistance aux Métaux Issus de Métatranscriptomes Eucaryotes de Sols." Thesis, Lyon, 2017. http://www.theses.fr/2017LYSE1056/document.

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Le sol est essentiel à toute société humaine notamment pour la production d'aliments. Son fonctionnement repose sur des réseaux d'interactions entre les éléments qui le composent et toute perturbation modifie ces réseaux. Les microorganismes eucaryotes représentent une composante importante de l'écosystème édaphique car ils sont impliqués dans des processus essentiels comme la régulation de populations de procaryotes. Mais paradoxalement, ils restent peu étudiés comparés aux bactéries notamment lorsqu'on s'intéresse aux cycles biogéochimiques autres que celui du carbone comme par exemple ceux
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Benning, Louise [Verfasser], Ingo [Akademischer Betreuer] Ahrens, and Marcus Sebastian [Akademischer Betreuer] Hortmann. "Droplet Digital PCR zur Quantifizierung von miR-21, miR-208a und miR-499 in der Diagnostik von Patienten mit funktionell relevanter koronarer Herzkrankheit." Freiburg : Universität, 2020. http://d-nb.info/1208623907/34.

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Bartolo, Jean-François. "Développement de sondes et de systèmes microfluidiques pour la détection de nouveaux biomarqueurs spécifiques." Thesis, Strasbourg, 2014. http://www.theses.fr/2014STRAF052/document.

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L’efficacité des traitements contre diverses pathologies dépend dans bien des cas de la précocité de la prise en charge des patients. Ce contexte pousse de nos jours les chercheurs à élaborer de nouvelles méthodes de diagnostic, généralement basées sur la détection de biomarqueurs spécifiques, permettant d’établir une corrélation entre un dérèglement moléculaire de l’organisme et la survenue d’une maladie. L’objectif de ces travaux était, par l’utilisation de la microfluidique digitale en gouttelettes, d’établir de nouvelles procédures simples et reproductibles, témoignant d’une sensibilité im
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Capitoli di libri sul tema "Digital droplet PCR"

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Mehle, Nataša, and Tanja Dreo. "Quantitative Analysis with Droplet Digital PCR." In Phytoplasmas. Springer New York, 2018. http://dx.doi.org/10.1007/978-1-4939-8837-2_14.

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Yu, Ming, Tai J. Heinzerling, and William M. Grady. "DNA Methylation Analysis Using Droplet Digital PCR." In Methods in Molecular Biology. Springer New York, 2018. http://dx.doi.org/10.1007/978-1-4939-7778-9_21.

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Parkin, Brian. "Rare Variant Quantitation Using Droplet Digital PCR." In Methods in Molecular Biology. Springer New York, 2018. http://dx.doi.org/10.1007/978-1-4939-8876-1_18.

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Vossen, Rolf H. A. M., and Stefan J. White. "Quantitative DNA Analysis Using Droplet Digital PCR." In Methods in Molecular Biology. Springer New York, 2016. http://dx.doi.org/10.1007/978-1-4939-6442-0_11.

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Gegevicius, Emilis, Karolis Goda, and Linas Mazutis. "CHAPTER 4. Droplet Gene Analysis – Digital PCR." In Soft Matter Series. Royal Society of Chemistry, 2020. http://dx.doi.org/10.1039/9781839162855-00089.

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Irfan, Mohammad, Uzma Rashid, Javid Iqbal Mir, Diaa Abd El Moneim, and Sheikh Mansoor. "Droplet Digital PCR in Fungal Pathogen Detection." In Conventional to Emerging Advances in Plant Disease Diagnosis and Pathogen Detection. CRC Press, 2025. https://doi.org/10.1201/9781003474036-5.

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Yan, Irene K., Rishabh Lohray, and Tushar Patel. "Droplet Digital PCR for Quantitation of Extracellular RNA." In Methods in Molecular Biology. Springer New York, 2018. http://dx.doi.org/10.1007/978-1-4939-7652-2_12.

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Ferracin, Manuela, and Massimo Negrini. "Quantification of Circulating MicroRNAs by Droplet Digital PCR." In Methods in Molecular Biology. Springer New York, 2018. http://dx.doi.org/10.1007/978-1-4939-7778-9_25.

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Bell, Avery Davis, Christina L. Usher, and Steven A. McCarroll. "Analyzing Copy Number Variation with Droplet Digital PCR." In Methods in Molecular Biology. Springer New York, 2018. http://dx.doi.org/10.1007/978-1-4939-7778-9_9.

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Härmälä, Suvi K., Robert Butcher, and Chrissy H. Roberts. "Copy Number Variation Analysis by Droplet Digital PCR." In Methods in Molecular Biology. Springer New York, 2017. http://dx.doi.org/10.1007/978-1-4939-7231-9_9.

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Atti di convegni sul tema "Digital droplet PCR"

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Xiao, Xiao, Le Gao, Yuanhua Yu, and Xinyu Chen. "Establishment of a Detection Methodology for CAR-T Cell Vector Copy Number Using Droplet Digital PCR." In 2024 IEEE International Conference on Manipulation, Manufacturing and Measurement on the Nanoscale (3M-NANO). IEEE, 2024. https://doi.org/10.1109/3m-nano61605.2024.10769736.

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Keo, Van Dong, Xuan Tran Hiep, Quoc Nguyen Banh, Tran Anh Son, and Duong Huyen Lynh. "Determination of Geometrical Parameters to Balance the Pressure Drop of Channels on a Microfluidic Chip." In 2024 International Conference on Machining, Materials and Mechanical Technologies. Trans Tech Publications Ltd, 2025. https://doi.org/10.4028/p-me24oh.

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Abstract (sommario):
In the past few years, micro-droplets have been widely used in diverse fields of biological and chemical research, spanning from drug delivery and material synthesis to point-of-care diagnostics, digital PCR, and single-cell analysis. Droplet-based microfluidics offers a powerful platform for conducting complex experiments, screening processes, and analyses with enhanced precision, efficiency, and versatility. While creating droplets with uniform sizes is a common objective of microfluidics, it is not limited to producing droplets of a single size per chip. Creating microdroplets with differen
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Zhang, Jie, Jiaqi Yan, Jinxian Wang, Jie Xu, Yilong Zhao, and Gangyin Luo. "Research on Droplet Digital PCR Amplification System." In 2021 IEEE 20th International Conference on Trust, Security and Privacy in Computing and Communications (TrustCom). IEEE, 2021. http://dx.doi.org/10.1109/trustcom53373.2021.00212.

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Meng, Xiangkai, Guangyong Jin, Yuanhua Yu, Jian Li, and Xue Tao. "Design of integrated droplet digital PCR gene chip." In Global Intelligent Industry Conference 2020, edited by Liang Wang. SPIE, 2021. http://dx.doi.org/10.1117/12.2590032.

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Yang, Jiayi, Boqiang Fu, Chunyan Niu, and Jing Wang. "Droplet digital PCR for quantitative detection of Escherichia coli." In INTERNATIONAL SYMPOSIUM ON THE FRONTIERS OF BIOTECHNOLOGY AND BIOENGINEERING (FBB 2019). AIP Publishing, 2019. http://dx.doi.org/10.1063/1.5110837.

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Valiakhmetova, E. R., D. S. Kopein, P. F. Snitko, and N. A. Litvinova. "PECULIARITIES OF PLASMID DNA MEASUREMENT BY DIGITAL DROPLET PCR." In XI МЕЖДУНАРОДНАЯ КОНФЕРЕНЦИЯ МОЛОДЫХ УЧЕНЫХ: БИОИНФОРМАТИКОВ, БИОТЕХНОЛОГОВ, БИОФИЗИКОВ, ВИРУСОЛОГОВ, МОЛЕКУЛЯРНЫХ БИОЛОГОВ И СПЕЦИАЛИСТОВ ФУНДАМЕНТАЛЬНОЙ МЕДИЦИНЫ. IPC NSU, 2024. https://doi.org/10.25205/978-5-4437-1691-6-223.

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Meng, Xiangkai, Guangyong Jin, Yuan Si, and Xue Tao. "Study on autofluorescence characteristics of micro droplet digital PCR chip." In 2020 International Conference on Optoelectronic Materials and Devices, edited by Siting Chen and Pei Wang. SPIE, 2021. http://dx.doi.org/10.1117/12.2592171.

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Shelton, Dawne, Jack Regan, George Karlin-Neumann, Jue Lin, and Elizabeth Blackburn. "Abstract LB-253: TRAPing telomerase activity using droplet digital PCR." In Proceedings: AACR 104th Annual Meeting 2013; Apr 6-10, 2013; Washington, DC. American Association for Cancer Research, 2013. http://dx.doi.org/10.1158/1538-7445.am2013-lb-253.

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So, Austin, Benjamin Hindson, Ryan Koehler, et al. "Abstract 3399: Detection of rare mutations in plasma by droplet digital PCR." In Proceedings: AACR 103rd Annual Meeting 2012‐‐ Mar 31‐Apr 4, 2012; Chicago, IL. American Association for Cancer Research, 2012. http://dx.doi.org/10.1158/1538-7445.am2012-3399.

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Huang, Jen-Yu, Shu-Sheng Lee, and Yu-Hsiang Hsu. "Development of an imaging method for quantifying a large digital PCR droplet." In SPIE BiOS, edited by Gerard L. Coté. SPIE, 2017. http://dx.doi.org/10.1117/12.2251801.

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