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Letteratura scientifica selezionata sul tema "Eagles minimal essential medium"

Autore: Grafiati

Pubblicato: 4 giugno 2021

Ultima modifica: 7 febbraio 2022

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Articoli di riviste sul tema "Eagles minimal essential medium"

Cinatl, J., J. Cinatl, V. Gerein, B. Kornhuber, and H. W. Doerr. "The establishment and characterization of mouse L-929 cells in protein-free Eagle's Minimal Essential Medium." Journal of Biological Standardization 16, no. 4 (January 1988): 249–57. http://dx.doi.org/10.1016/0092-1157(88)90012-1.

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Hnat, Michael, and Roger E. Bawdon. "Transfer of Meropenem in the ex Vivo Human Placenta perfusion Model." Infectious Diseases in Obstetrics and Gynecology 13, no. 4 (2005): 223–27. http://dx.doi.org/10.1155/2005/961356.

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Objectives.To determine maternal-fetal transplacental passage of meropenem in the ex vivo human perfusion model.Study design.Term placentae (n= 6) were collected immediately after delivery. A single cotyledon was localized, perfused and stabilized with physiologic Eagles minimal essential medium containing 3% bovine albumin and heparin as described by Chalier (Chalier JC. Criteria for evaluating perfusion experiments and presentation results. Contrib Gynecol Obstet 1985; 13:32–39). Meropenem was added to the maternal medium in concentrations similar to maternal serum peak and trough levels, th

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Seino, Satoshi, Yasuo Imoto, Tomoya Kosaka, Tomoki Nishida, Takashi Nakagawa, and Takao A. Yamamoto. "Antiviral Activity of Silver Nanoparticles Immobilized onto Textile Fabrics Synthesized by Radiochemical Process." MRS Advances 1, no. 11 (2016): 705–10. http://dx.doi.org/10.1557/adv.2016.43.

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ABSTRACTAntiviral activity of metallic Ag nanoparticles immobilized on textile fabrics were investigated. The Ag nanoparticles synthesized by radiochemical process are firmly immobilized on the surface of support textile fabrics of cotton. Small Ag particles of about 2–4 nm were observed together with relatively large particles of more than 10 nm. The Ag nanoparticles showed antiviral activity against Influenza A and Feline Calicivirus. The antiviral activity significantly depended on the concentration of the Eagle’s minimal essential medium. It was implied that the surface passivation by inhi

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Tung, L. C. "Immunocytochemistrical Studies on Cytoskeletal Change in Cultured Cardiomyocyte of Tilapia." Microscopy and Microanalysis 6, S2 (August 2000): 888–89. http://dx.doi.org/10.1017/s143192760003693x.

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In the present study, the myofibril regeneration in the long-term cultured fish cardiomyocytes was studied with immunocytochemistry.Adult Tilapia heart was dissociated into a single-cell suspension with collagenase and protease-minced tissue method. The culture medium was Eagle's minimal essential medium (MEM) with Earle's salts, supplemented with 10% fetal calf serum, 1 x nonessential amino acid mixture, 100 IU/ml penicillin G, and 100 μg/ml streptomycin. The cultured cells were grown in a humidified CO2 incubator at 28°Cand in a medium without glutamine for eliminating fibroblast contaminati

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Heredia, Nelly Solfania, Ann Sabrina Ávila, and Luz Elena Velásquez. "In vitro culture of L3 larvae of nematodes obtained from the African giant snail Lissachatina fulica (Mollusca: Gastropoda) in Santa Fe de Antioquia." Biomédica 38 (August 1, 2018): 24–29. http://dx.doi.org/10.7705/biomedica.v38i3.3408.

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Introducción. Más de 170 municipios colombianos están invadidos por Lissachatina fulica, caracol africano que puede portar larvas de nematodos de interés en salud humana y veterinaria. Los parásitos entran al caracol huésped intermediario en el estadio de larva L1, y allí cambian a L2 y L3, formas estas capaces de infectar a vertebrados.Objetivo. Estandarizar el cultivo in vitro de las L3 portadas por especímenes de L. fulica recolectados en Santa Fe de Antioquia.Materiales y métodos. Entre julio y noviembre de 2014 se recolectaron 10 caracoles, se sacrificaron y se digirieron con ácido clorhí

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Mouat, M. F., A. C. Cantrell, and K. L. Manchester. "Membrane potential of rat hepatoma cells in culture: Influence of factors affecting amino acid transport." Bioscience Reports 15, no. 4 (August 1, 1995): 173–84. http://dx.doi.org/10.1007/bf01540451.

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The effect has been studied of various media, hormones and of amino acids on the membrane potential of rat hepatoma cells in culture measured by microelectrode impalement. Cells in Eagle's minimal essential medium plus 5% serum had a value which varied daily from about 5–8 mV, inside negative. The membrane potential of rat hepatocytes was measured to be 8.7 ± 0.2mV, inside negative. The membrane potential of the hepatoma cells was decreased by insulin and increased by glucagon. Membrane potential was unaffected by change of medium to Hanks' or Earle's balanced salt solutions or deprivation of

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Al-Nazhan, Saad, and Afaf Al-Nasser. "Viability of Human Periodontal Ligament Fibroblasts in Tissue Culture After Exposure to Different Contact Lens Solutions." Journal of Contemporary Dental Practice 7, no. 4 (2006): 37–44. http://dx.doi.org/10.5005/jcdp-7-4-37.

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Abstract Aim The viability of the periodontal ligament (PDL) cells is critical for successful healing of replanted avulsed teeth. Viability is primarily dependent on the duration of the extra-alveolar time and storage medium used to preserve teeth. Several storage media have been suggested but milk ranks highest. It would be desirable to evaluate other media as a suitable alternative for milk. The purpose of this study was to determine the viability of human PDL fibroblasts and their morphology after storage in different types of contact lens solutions. Methods and Materials PDL fibroblasts we

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Kellermayer, Miklós S. Z., Tamás Henics, György Szücs, and Gerald H. Pollack. "Electron microscopic analysis of the microtubular structure of HEp-2 cells." Proceedings, annual meeting, Electron Microscopy Society of America 48, no. 3 (August 12, 1990): 470–71. http://dx.doi.org/10.1017/s0424820100159898.

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The HEp-2 cell line was first established by Moore, et al. and today it serves as a widely used subject for a large variety of microbiological and cell-biological experiments. A tubular structure of unknown origin and function has been described first in virally infected, and later in uninfected HEp-2 cells. However, this tubular structure has not been further analyzed. We performed transmission (TEM) and whole cell mount electron microscopic studies of monolayer HEp-2 cells to morphologically describe the structure and relate it to other organelles of the cell.For embedding, HEp-2 cells were

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Tifrea, Delia F., Pooja Ralli-Jain, Sukumar Pal, and Luis M. de la Maza. "Vaccination with the Recombinant Major Outer Membrane Protein Elicits Antibodies to the Constant Domains and Induces Cross-Serovar Protection against Intranasal Challenge with Chlamydia trachomatis." Infection and Immunity 81, no. 5 (March 11, 2013): 1741–50. http://dx.doi.org/10.1128/iai.00734-12.

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ABSTRACTTo determine the ability of the major outer membrane protein (MOMP) to elicit cross-serovar protection, groups of mice were immunized by the intramuscular (i.m.) and subcutaneous (s.c.) routes with recombinant MOMP (rMOMP) fromChlamydia trachomatisserovars D (UW-3/Cx), E (Bour), or F (IC-Cal-3) orChlamydia muridarumstrain Nigg II using CpG-1826 and Montanide ISA 720 VG as adjuvants. Negative-control groups were immunized i.m. and s.c. withNeisseria gonorrhoeaerecombinant porin B (Ng-rPorB) or i.n. with Eagle's minimal essential medium (MEM-0). Following vaccination, the mice developed

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Reader, S. C. J., B. Davison, J. G. Ratcliffe, and W. R. Robertson. "Measurement of low concentrations of bovine thyrotrophin by iodide uptake and organification in porcine thyrocytes." Journal of Endocrinology 106, no. 1 (July 1985): 13—NP. http://dx.doi.org/10.1677/joe.0.1060013.

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ABSTRACT Thyrocytes isolated from porcine thyroids by mechanical and enzymatic dispersion and cultured in Eagle's minimal essential medium, supplemented with 5% (v/v) fetal calf serum, glutamine and cortisol, formed a continuous monolayer within 48 h. This monolayer was without cytochemical peroxidase and diaphorase (NADPH reoxidation) activity. In the presence of bovine thyrotrophin (bTSH; 50 mu./l) the cells developed a follicular-like architecture which was maximal at 4 days before reverting back to a uniform monolayer at 6 days. There were no detectable changes in the total DNA content ove

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Più fonti

Tesi sul tema "Eagles minimal essential medium"

Lei, Jie. "The role of antioxidants in the hydrogen peroxide-induced opacification of sheep lens." Master's thesis, Lincoln University. Agriculture and Life Sciences Division, 2006. http://theses.lincoln.ac.nz/public/adt-NZLIU20070517.162145/.

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The lens of the eye needs to be transparent with a high refractive index to focus images on the retina. In cataracts the lens becomes opaque, eventually leading to blindness. There are many possible causes of cataract but a lot of evidence implicates oxidative damage as contributing to opacification. This includes epidemiological studies showing that diets rich in antioxidants lowered the prevalence of cataract. This research tested the hypothesis that if cataracts were at least partially caused by oxidative damage then their progression would be slowed by application of antioxidants. The anti

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