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Tesi sul tema "Embryonic stem cells"

Autore: Grafiati

Pubblicato: 4 giugno 2021

Ultima modifica: 25 luglio 2025

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1

Nortjé, Nico. "The moral status of embryonic stem cell research in the South African context /." Link to the online version, 2007. http://hdl.handle.net/10019.1/1372.

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2

Harun, Rosliah. "Derivation of trophoblast stem cells from human embryonic stem cells." Thesis, University of Sheffield, 2004. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.414643.

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3

Montgomery, Sarah Lynn. "Impedance measurement system for embryonic stem cell and embryoid body cultures." Thesis, Atlanta, Ga. : Georgia Institute of Technology, 2008. http://hdl.handle.net/1853/24661.

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4

Gilner, Jennifer Bushman Kirby Suzanne Lee. "Enrichment of therapeutic hematopoietic stem cell populations from embryonic stem cells." Chapel Hill, N.C. : University of North Carolina at Chapel Hill, 2007. http://dc.lib.unc.edu/u?/etd,1232.

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Thesis (Ph. D.)--University of North Carolina at Chapel Hill, 2007.<br>Title from electronic title page (viewed Mar. 26, 2008). "... in partial fulfillment of the requirements for the degree of Doctor of Philosophy in the Department of Pathology and Laboratory Medicine." Discipline: Pathology and Laboratory Medicine; Department/School: Medicine.

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5

Jurczak, Daniel. "Stemness in human embryonic stem cells." Thesis, University of Skövde, School of Life Sciences, 2009. http://urn.kb.se/resolve?urn=urn:nbn:se:his:diva-3509.

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<p>Stem cells are cells that have a unique ability to divide for an indefinite period. Additionally, they can give rise to a plethora of specialized cell types. The advent of high-throughput technologies made it possible to investigate gene expression on a large scale. This enabled scientists to perform comprehensive gene profiling studies of stem cells. Several authors have suggested that there might be a common set of genes that control the stemness of stem cells. In this study, we suggest that ”stemness” genes that are related to ”stemness” characteristics show a statistically significant d

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6

Nussbaum, Jeannette. "Embryonic stem cells for myocardial infarct repair /." Thesis, Connect to this title online; UW restricted, 2004. http://hdl.handle.net/1773/6312.

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7

Noisa, Parinya. "Characterization of neural progenitor/stem cells derived from human embryonic stem cells." Thesis, Imperial College London, 2010. http://hdl.handle.net/10044/1/5712.

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Human embryonic stem cells (hESCs) are able to proliferate indefinitely without losing their ability to differentiate into multiple cell types of all three germ layers. Due to these fascinating properties, hESCs have promise as a robust cell source for regenerative medicine and as an in vitro model for the study of human development. In my PhD study, I have investigated the neural differentiation process of hESCs using our established protocol, identified characteristics associated with each stage of the differentiation and explored possible signalling pathways underlying these dynamic changes

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8

Bigdeli, Narmin. "Derivation, characterization and differentiation of feeder-free human embryonic stem cells /." Göteborg : Department of Clinical Chemistry and Transfusion Medicine, Institute of Biomedicine at Sahlgrenska Academy, University of Gothenburg, 2010. http://hdl.handle.net/2077/22353.

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9

Lu, Xibin, and 盧希彬. "Quantitative characterization of mouse embryonic stem cell state transition." Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 2014. http://hdl.handle.net/10722/208049.

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10

Bowles, K. M. "Generation of haematopoietic cells from human embryonic stem cells." Thesis, University of Cambridge, 2006. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.596829.

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Culture of hESCs on murine stromal layers or in stromal free conditions as embryoid bodies results in low levels of haematopoietic cells. Here it is demonstrated that overexpression of the transcription factor HOXB4 considerably augments haematopoetic development of hESCs. Stable HOXB4 expressing hESC clones were generated by lipofection and could be maintained in the undifferentiated state for prolonged passages. Moreover, differentiation of hESCs as embryoid bodies in serum containing medium without the use of additional of cytokines led to sequential expansion of first erythroid then myeloi

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11

Hayashi, Hideki. "Meningeal cells induce dopaminergic neurons from embryonic stem cells." Kyoto University, 2008. http://hdl.handle.net/2433/124217.

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12

Ericson, Robin J. "Bridging solutions to the religion and science conflict over human embryonic stem cell research." Fairfax, VA : George Mason University, 2007. http://hdl.handle.net/1920/2926.

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Thesis (Ph. D.)--George Mason University, 2007.<br>Title from PDF t.p. (viewed Jan. 17, 2008). Thesis director: Richard E. Rubenstein. Submitted in partial fulfillment of the requirements for the degree of Doctor of Philosophy in Conflict Analysis and Resolution. Vita: p. 228. Includes bibliographical references (p. 222-227). Also available in print.

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13

Joannides, Alexis. "Neural differentiation of human embryonic stem cells." Thesis, University of Cambridge, 2009. https://www.repository.cam.ac.uk/handle/1810/252121.

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Human embryonic stem cells (hESCs) are a potential source of defined cell types for studying early human development and application in regenerative medicine. Realising this potential requires a number of challenges to be overcome. The experimental findings reported represent a systematic approach in establishing controlled and standardised conditions for differentiating hESCs down the neural lineage, and characterising neural derivatives both <i>in vitro</i> and <i>in vivo.</i> Human embryonic stem cell cultures were established from two independently-derived liens, H9 and UES9. A novel, effi

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14

Antonica, Francesco. "Modelling thyroid embryogenesis using embryonic stem cells." Doctoral thesis, Universite Libre de Bruxelles, 2013. http://hdl.handle.net/2013/ULB-DIPOT:oai:dipot.ulb.ac.be:2013/209551.

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Congenital hypothyroidism (CH) is the most frequent of the rare endocrine diseases (e.g. Addison's disease, Cushing's syndrome, Congenital adrenal hyperplasia.), which affects 1:2000 – 4000 newborns. If not immediately diagnosed after birth, thyroid hormones deficiency causes severe defects in brain and skeletal development leading to a complex clinical scenario called cretinism. CH can be due to a defective synthesis of thyroid hormones (dyshormonogenesis) or an abnormal embryonic development of the gland. Data obtained using knockout mouse models have shown the pivotal role of four specific

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15

Gordon-Keylock, Sabrina Anne Megan. "Haematopoietic differentiation of murine embryonic stem cells." Thesis, University of Edinburgh, 2009. http://hdl.handle.net/1842/29123.

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This thesis aimed to determine which subregions of the E10.5 aorta-gonad-mesonephros (AGM) were responsible for the haematopoietic enhancing effects that primary AGM regions had on differentiating ES cells. To this end, a novel co-culture system has been established to test the enhancing effects of a panel of clonal stromal cell lines derived from different subregions of the midgestational AGM. Three stromal cell lines derived from the dorsal aorta and surrounding mesenchyme (AM) subregion of the AGM were able to significantly enhance the frequency of ES cell derived multipotent haematopoietic

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16

Lake, Julie-anne. "Differentiation of pluripotential murine embryonic stem cells." Adelaide Thesis (Ph.D.) -- University of Adelaide, Department of Biochemistry, 1996. http://hdl.handle.net/2440/18794.

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17

Both, Sanne Karijn. "Embryonic stem cells in bone tissue engineering." Enschede : University of Twente [Host], 2008. http://doc.utwente.nl/58765.

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18

Mak, Shiu-kwong Thomas, and 麥肇鑛. "Modeling diabetic cardiomyopathy using embryonic stem cells." Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 2013. http://hdl.handle.net/10722/193562.

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Abstract (sommario):

Diabetic cardiomyopathy (DCM), a disorder of the heart muscle, is one of the major and most rampant culprits claiming thousands and thousands of lives around the globe every year by interfering with the blood circulation and causing the development of heart failure eventually. The progression of the disease is asymptomatic and having a long latent period, and it is characterized functionally by ventricular dilation, diastolic dysfunction, interstitial fibrosis and cardiomyocytes hypertrophy. It was suggested the pathogenesis of the disease and the related complications are related to the effec

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19

Kleinert, Fanni. "Nuclear architecture in differentiating embryonic stem cells." Thesis, University of Manchester, 2015. https://www.research.manchester.ac.uk/portal/en/theses/nuclear-architecture-in-differentiating-embryonic-stem-cells(fb45d7d6-69d7-4b63-8785-503eca352131).html.

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Gene expression is regulated at various levels, such as transcription, RNA transport and translation. Additionally, it has been shown that chromatin structure, location and dynamics also have an important role in gene expression control. While active gene regions are strongly associated with an open chromatin structure at the surface of the chromosome territory (CT) and a location in the nuclear interior, inactive gene regions seem to be related with a closed structure within the CT and a position at the nuclear periphery. However, it is still unclear how these features are regulated. Importan

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20

Rugg-Gunn, Peter. "Epigenetic status of human embryonic stem cells." Thesis, University of Cambridge, 2006. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.614294.

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21

Karan, Priyanka. "Embryonic stem cells alter cardiomyocyte electrophysiological properties." Thesis, Atlanta, Ga. : Georgia Institute of Technology, 2008. http://hdl.handle.net/1853/24691.

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22

Lin, Wenyu. "Investigating the immunomodulatory properties of human embryonic stem cell-derived mesenchymal stem cells." Thesis, Imperial College London, 2010. http://hdl.handle.net/10044/1/7060.

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Abstract (sommario):

The immunosuppressive property of mesenchymal stem cells (MSC) has been utilised to ameliorate autoimmune reactions such as graft-versus-host disease. However, variation exists in primary MSC isolated due to differences in donor age and tissue of origin. Alternatively, human embryonic stem cells (hESC) can be differentiated to homogeneous populations of MSC (hESCMSC), thus providing an unlimited source of MSC for cell therapy. In this study, the immunomodulatory properties of two hESC-MSC lines, hESC-MSC1 and hESC-MSC2, were compared with adult bone marrow-derived MSC (BM-MSC) and neonatal for

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23

Vitillo, Loriana. "Focal adhesion kinase regulation of human embryonic stem cells." Thesis, University of Manchester, 2014. https://www.research.manchester.ac.uk/portal/en/theses/focal-adhesion-kinase-regulation-of-human-embryonic-stem-cells(31052725-50a4-4d34-a1eb-018be57986af).html.

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Abstract (sommario):

Undifferentiated human embryonic stem cells (hESCs) grow on the extracellular matrix (ECM) substrate fibronectin (FN) in defined feeder-free conditions. The ECM is part of the hESCs pluripotent niche and supports their maintenance, but the contribution to survival remains to be elucidated. Understanding the mechanism of survival is particularly crucial in hESCs, since it affects their expansion in cell culture and ultimately translation of research to the clinic. HESCs bind to FN mainly via alpha5β1- integrin, known to be upstream of important survival cascades in other cell types. However, it

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24

Chen, Shi. "Cryopreservation of human embryonic stem cells and hepatocytes." Thesis, University of Oxford, 2013. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.711665.

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25

Shah, Nadia Nisa. "Human embryonic stem cells : prospects for pancreatic β-cell differentiation". Thesis, University of Manchester, 2008. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.495052.

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The focus of this thesis was to explore different strategies in trying to generate putative pancreatic β-cells using one of the initial Wisconsin H7 hES cell lines. Prior to this, human pancreas development was assessed during the first trimester of pregnancy in an attempt to determine the spatial and temporal expression of development and mature pancreatic β-cell markers during this period. Spontaneous differentiation of hES cells can be induced by the formation of embryoid bodies (EBs) in suspension culture. EBs began to express markers of pancreatic β-cell development and function at a mole

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26

Faddah, Dina Adel. "Single-cell analyses of cellular reprogramming and embryonic stem cells." Thesis, Massachusetts Institute of Technology, 2014. http://hdl.handle.net/1721.1/89941.

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Thesis: Ph. D., Massachusetts Institute of Technology, Department of Biology, 2014.<br>Vita. Cataloged from student-submitted PDF version of thesis.<br>Includes bibliographical references.<br>Three years before the start of this thesis, Yamanaka and Takahashi published a groundbreaking paper entitled "Induced of pluripotent stem cells from mouse embryonic and adult fibroblast cultures by defined factors." A mere two scientists reprogrammed somatic cells to an embryonic stem-cell like state (termed induced pluripotent stem cells, iPSCs) by simply overexpressing four transcription factors: Oct4,

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27

Ngangan, Alyssa V. "Bioactive factors secreted by differentiating embryonic stem cells." Diss., Georgia Institute of Technology, 2011. http://hdl.handle.net/1853/44913.

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Current therapeutic strategies to stimulate endogenous angiogenic processes within injured tissue areas are typically based on introducing exogenous pro-angiogenic molecules or cell populations. Stem cell transplantation for angiogenic therapy aims to deliver populations of cells that secrete angiogenic factors and/or engraft in the new branching vasculature within the damaged tissue. Utilizing stem or progenitor cells has been shown to induce a rather robust angiogenic response despite minimal repopulation of the host vasculature, suggesting that stem cells may provide paracrine factors tha

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28

Nair, Rekha. "Acellular matrices derived from differentiating embryonic stem cells." Diss., Georgia Institute of Technology, 2009. http://hdl.handle.net/1853/37170.

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Embryonic stem cells (ESCs) can differentiate into all somatic cells, and as such, are a promising cell source for therapeutic applications. In vitro, ESCs spontaneously differentiate via the aggregation of cells into embryoid bodies (EBs), which recapitulate aspects of early embryogenesis and harbor a unique reservoir of cues critical for tissue formation and morphogenesis. Embryonic healing responses employ similar intrinsic machinery used for tissue development, and these morphogenic cues may be captured within the EB microenvironment. Recent studies have shown that when injected into in

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29

Cho, Ting-yin. "Conversion from mouse embryonic to extra-embryonic endoderm stem cells reveals distinct differentiation capacities of pluripotent stem cell states." Thesis, University of Cambridge, 2013. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.607991.

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30

Blyszczuk, Przemyslaw. "Differentiation of embryonic stem cells into pancreatic insulin-producing cells." [S.l. : s.n.], 2004. http://deposit.ddb.de/cgi-bin/dokserv?idn=97560032X.

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31

Kim, Peter Tae Wan. "Directed differentiation of endodermal cells from mouse embryonic stem cells." Thesis, University of British Columbia, 2008. http://hdl.handle.net/2429/771.

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Abstract (sommario):

Pluripotent embryonic stem cells hold a great promise as an unlimited source of tissue for treatment of chronic diseases such as Type 1 diabetes and chronic liver disease. Various attempts have been made to produce cells that can serve as precursors for pancreas and liver. By using all-trans-retinoic acid, basic fibroblast growth factor, dibutyryl cAMP, and cyclopamine, an attempt has been made to produce definitive endoderm and subsequently cells that can serve as pancreatic and hepatocyte precursors from mouse embryonic stem cells. By using retinoic acid and basic-FGF, in the absence of embr

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32

Uroić, Daniela Sonja. "Differentiation of embryonic stem cells towards pancreatic β-like cells". Thesis, University of Aberdeen, 2011. http://digitool.abdn.ac.uk:80/webclient/DeliveryManager?pid=167694.

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Embryonic stem (ES) cells were used as a model system to understand the signalling events in pancreas development. ES cells were differentiated through known precursor stages towards the tissue of interest in order to recapitulate development in vitro. Thus, protocols directing differentiation of mouse ES cells towards definitive endoderm and pancreatic β-cells were developed. A combination of activin A and bone morphogenic protein 4 resulted in a population of enriched cells expressing genetic markers of definitive endoderm. In vitro differentiation of ES cells into functional pancreatic β-ce

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33

Neilson, Kirstie Jane. "Differentiation of mouse embryonic stem cells into endothelial progenitor cells." Thesis, University of Sheffield, 2008. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.500200.

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34

Milne, Helen Marie. "Generation of insulin-expressing cells from mouse embryonic stem cells." Thesis, King's College London (University of London), 2005. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.417362.

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35

Thomas, Paul Quinton. "Homeobox gene expression in murine embryonic stem cells." Title page, contents and summary only, 1996. http://web4.library.adelaide.edu.au/theses/09PH/09pht4608.pdf.

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Includes bibliographies. Aims to identify homeobox genes which may have a developmental role during early embryogenesis by the characterization of homeobox gene expression in undifferentiated ES cells, and in a range of differentiated ES cell derivatives.

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36

Lee, Yee-ki Carol, and 李綺琪. "Pluripotent stem cells for cardiac regeneration." Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 2011. http://hub.hku.hk/bib/B46918462.

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37

Meyer, Jason S. "Characterization and therapeutic transplantation of stem cells /." free to MU campus, to others for purchase, 2004. http://wwwlib.umi.com/cr/mo/fullcit?p3164528.

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38

Ismail, Siti N. "Stem cell bioprocessing : the bioengineering of lung epithelium in 3D from embryonic stem cells." Thesis, Imperial College London, 2009. http://hdl.handle.net/10044/1/9013.

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Stem cell therapies and tissue engineering strategies are required for the clinical treatment of respiratory diseases. Previous studies have established protocols for the differentiation of airway epithelium from stem cells but have involved costly and laborious culture methods. The aim of this thesis was to achieve efficient and reproducible maintenance and differentiation of embryonic stem cells to airway epithelium, in 2D and 3D culture, by developing appropriate bioprocessing technology. Firstly, the 2D differentiation process of human and murine ES cells into pulmonary epithelial cells wa

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39

Sargent, Carolyn Yeago. "Effects of hydrodynamic culture on embryonic stem cell differentiation: cardiogenic modulation." Diss., Georgia Institute of Technology, 2010. http://hdl.handle.net/1853/34710.

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Stem and progenitor cells are an attractive cell source for the treatment of degenerative diseases due to their potential to differentiate into multiple cell types and provide large cell yields. Thus far, however, clinical applications have been limited due to inefficient differentiation into desired cell types with sufficient yields for adequate tissue repair and regeneration. The ability to spontaneously aggregate in suspension makes embryonic stem cells (ESCs) amenable to large-scale culture techniques for the production of large yields of differentiating cell spheroids (termed embryoid b

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40

Markoullis, Kyriaki. "Mycoplasma contamination of murine embryonic stem (mES) cells." Diss., lmu, 2008. http://nbn-resolving.de/urn:nbn:de:bvb:19-99459.

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41

Randle, Wesley Liam. "Tissue engineering of bone from embryonic stem cells." Thesis, Imperial College London, 2006. http://hdl.handle.net/10044/1/11880.

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42

Cho, Sarah K. "Lymphohematopoietic differentiation from embryonic stem cells in vitro." Thesis, National Library of Canada = Bibliothèque nationale du Canada, 2001. http://www.collectionscanada.ca/obj/s4/f2/dsk3/ftp05/NQ63681.pdf.

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43

Hollands, Peter. "Differentiation and grafting of embryonic haemopoietic stem cells." Thesis, University of Cambridge, 1987. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.330219.

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44

Meek, Stephen Earl. "Experimental approaches to establish rat embryonic stem cells." Thesis, University of Edinburgh, 2011. http://hdl.handle.net/1842/5040.

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The rat has been an established experimental animal model within many areas of biological investigation for over one hundred years due to its size, breeding characteristics, and knowledge of its physiology and behaviour. In recent years its status as a leading biomedical model has been somewhat surpassed by the mouse. This is largely the result of the isolation and application of mouse embryonic stem (ES) cells. Mouse ES cells have the capacity for unlimited self-renew in vitro whilst maintaining pluripotency and germline competence, and most importantly are amenable to sophisticated reverse g

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45

Tilgner, Katarzyna. "Derivation of oocytes from human embryonic stem cells." Thesis, University of Newcastle Upon Tyne, 2009. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.512211.

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46

Sutha, Ken. "Osteoinductive material derived from differentiating embryonic stem cells." Diss., Georgia Institute of Technology, 2012. http://hdl.handle.net/1853/51722.

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The loss of regenerative capacity of bone, from fetal to adult to aged animals, has been attributed not only to a decline in the function of cells involved in bone formation but also to alterations in the bone microenvironment that occur through development and aging, including extracellular matrix (ECM) composition and growth/trophic factor content. In the development of novel treatments for bone repair, one potential therapeutic goal is the restoration of a more regenerative microenvironment, as found during embryonic development. One approach to creating such a microenvironment is through t

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47

Coe, Torres Davi. "Understanding H3K36 methyltransferases in mouse embryonic stem cells." Doctoral thesis, Saechsische Landesbibliothek- Staats- und Universitaetsbibliothek Dresden, 2014. http://nbn-resolving.de/urn:nbn:de:bsz:14-qucosa-144706.

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Methylation of histone 3 (H3) at lysine 36 (K36) has been implicated in several biological processes, such as DNA replication, DNA repair, and transcription. To date, at least eight distinct mammalian enzymes have been described to methylate H3K36 in vitro and/or in vivo. In this work, Set2, Nsd1, and Nsd3 Venus tagged proteins were successfully expressed in mouse embryonic stem cells and, then, analyzed by confocal microscopy, mass spectrometry (MS), and chromatin immunoprecipitation sequencing (ChIP-seq). MS analysis revealed that Setd2, Nsd1, and Nsd3 do not associate in protein complexes w

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48

Wan, Chen-rei. "Characterization of the cardiogenesis of embryonic stem cells." Thesis, Massachusetts Institute of Technology, 2010. http://hdl.handle.net/1721.1/65283.

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Thesis (Ph. D.)--Massachusetts Institute of Technology, Dept. of Mechanical Engineering, February 2011.<br>Cataloged from PDF version of thesis.<br>Includes bibliographical references (p. 114-127).<br>Cardiovascular diseases persist as the leading cause of mortality worldwide. Stem cell therapy, aimed to restore contractility and proper vasculature, has gained considerable attention as an attractive therapeutic option. However, proper cell differentiation, survival and integration in an infarcted zone remain elusive. This thesis aims to utilize in vitro techniques to obtain a systematic charac

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49

Tailor, Jignesh Kishor. "Human neuroepithelial stem cells from the embryonic hindbrain." Thesis, University of Cambridge, 2013. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.607958.

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50

Blair, Kathryn Lee. "Properties of embryonic stem cells from Rattus norvegicus." Thesis, University of Cambridge, 2012. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.610069.

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