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Tesi sul tema "Enzyme technology"

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1

Hall, Geoffrey F. "Organic phase enzyme electrodes." Thesis, Cranfield University, 1990. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.278720.

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2

Molawa, Letshego Gloria. "SphereZyme (TM) technology for enhanced enzyme immobilisation application in biosensors." Thesis, Rhodes University, 2011. http://hdl.handle.net/10962/d1004048.

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Self-immobilisation enzyme technologies, such as SphereZyme™, suffer from the lack of applicability to hydrolyse large substrates. Solid support immobilisation is usually a method of choice, to produce a stable biocatalyst for large substrates hydrolysis in the industry. In order to investigate this limitation, a commercial protease called Alcalase® was chosen as a model enzyme due to its natural activity (hydrolysis of large substrates-proteins). Prior to immobilising through the SphereZyme™ technology, Alcalase® was partially purified through dialysis followed by CM Sepharose™ FF cation exch
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3

Chimphango, Annie Fabian Abel. "Development of enzyme technology for modification of functional properties of xylan biopolymers." Thesis, Stellenbosch : University of Stellenbosch, 2010. http://hdl.handle.net/10019.1/5448.

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Thesis (PhD (Process Engineering))--University of Stellenbosch, 2010.<br>ENGLISH ABSTRACT: There is growing interest to utilise xylan as speciality biopolymers in similar ways as high molecular weight polysaccharides such as starch and cellulose. The need to utilise xylan as alternative to cellulose and starch has increased because the cellulose and starch have many other competing uses. Unlike cellulose and starch, xylans are heteropolymers with higher degree of substitution and are of lower molecular mass and therefore, do not readily become insoluble to form hydrogels and biofilms. Conseque
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4

Silva, Meliza Lindsay Rojas. "Fruit beverages processed using ultrasound technology: physical properties, stability and enzyme inactivation." Universidade de São Paulo, 2016. http://www.teses.usp.br/teses/disponiveis/11/11141/tde-06012017-150144/.

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This work studied the improvement of stability, physical properties and enzymatic inactivation in fruit beverages by applying the ultrasound technology (US). In the first part, the effect of the US application on peach juice processing was evaluated. The macroscopic changes on pulp sedimentation stability, turbidity, colour and rheological properties were evaluated. As a result, it was demonstrated that the improvement in each of the properties evidenced at the macroscopic level involves interaction of complex mechanisms which depend directly on changes at the microscopic level, such as the st
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5

Canning, Anne. "Enzyme responsive surfaces : towards a smart cell-material interface." Thesis, University of Nottingham, 2018. http://eprints.nottingham.ac.uk/49954/.

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Enzymes are promising stimuli for the development of responsive biomaterials for biomedical applications. Enzymes are inherently present in the biological environment thus cleverly designed materials for biomedical applications may require no external stimuli to ellicit the required material response. They have been targeted as stimuli in self assembly of bulk materials owing to the material changes in chemical composition afforded by the enzyme interaction. The first examples of autonomous self-regulated drug delivery systems have been reported via the development of reversible enzyme respons
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6

Zhao, Min Chemistry Faculty of Science UNSW. "A fill and flow channel enzyme biosensor." Awarded by:University of New South Wales, 2004. http://handle.unsw.edu.au/1959.4/38333.

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7

Sato, Hiroaki 1962. "Using human metabolic enzyme profiling as an innovative technology in the drug development process." Thesis, Massachusetts Institute of Technology, 2000. http://hdl.handle.net/1721.1/9205.

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Thesis (M.B.A.)--Massachusetts Institute of Technology, Sloan School of Management, 2000.<br>Also available online at the DSpace at MIT website.<br>Includes bibliographical references (leaves 47-50).<br>Undesirable pharmacokinetic properties, such as poor bioavailability and drug-drug interactions, have been one of major reasons for the failure of new pharmaceuticals in clinical trials. These dropout risks can be reduced by early knowledge of human pharmacokinetics in the drug discovery process. Although new drug candidates have been first tested in animal-based systems, the prediction of huma
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8

Li, Yuxiu. "Technology of enzyme extraction of Gynostemma pentaphyllum saponins and studies on its hypoglycemic activity." Магістерська робота, Kyiv National University of Technology and Design, 2021. https://er.knutd.edu.ua/handle/123456789/19271.

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In this master's thesis, the composition of saponins in extracts of Gynostemma pentaphyllum obtained by enzymatic extraction has been determined. The HPLC method was used for the simultaneous analysis of hypenosides in medicinal plant materials of different periods and places of growth. The results showed that the highest content of hypenosides was noted in August, the lowest in May; the composition and content of hypenosides from different production zones in the same growing season were different. The optimal parameters of extraction using hypenosidase are as follows: the time of enzymatic h
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9

Sailis, Fiammetta. "Detection of miRNA by SMART technology." Thesis, University of Edinburgh, 2017. http://hdl.handle.net/1842/28891.

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Aberrant expression of short non-coding micro RNAs (miRNA) in many human diseases, along with remarkable stability in physiological media, has made them attractive clinical biomarkers. In particular, miRNA-122 is substantially elevated in plasma of patients with established drug-induced liver injury and can also be used to identify early liver injury when current markers, such as alanine aminotransferase (ALT), still show normal levels. The development of a rapid test for miRNA-122 e.g. in drug poisoning would allow earlier and more sensitive clinical diagnosis of liver injury. Nucleic acids a
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10

Erbeldwger, Markus. "Enzymatic solid-to-solid peptide synthesis : from kinetics to synthesis of z-aspartame on preparative scales." Thesis, University of Strathclyde, 2000. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.366997.

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11

Yoo, Juhyun. "Effect of enzyme application in temper water on wheat milling." Thesis, Manhattan, Kan. : Kansas State University, 2007. http://hdl.handle.net/2097/524.

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12

Johnston, David A. "The use of metal evaporation in the design and manufacture of enzyme electrodes." Thesis, University of the West of Scotland, 2000. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.323763.

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13

Rui, Xin. "Assessment of the angiotensin I-converting enzyme (ACE) inhibitory properties of bean protein hydrolysates." Thesis, McGill University, 2013. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=114468.

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The prevalence of hypertension during the last two decades has led to an intensive search for novel angiotensin I-converting enzyme (ACE) inhibitory peptides from food protein sources. Dry beans (Phaseolus vulgaris) serve as an important protein source in the human diet. However, their physiological and functional properties are poorly understood. Therefore, this thesis was undertaken to investigate the in vitro ACE inhibitory activities of nine varieties of dry beans that are largely grown in Canada.A preliminary study was carried out to compare protein profiles, including composition, therma
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14

Jenidi, Youla. "Bioremediation of estrone from water matrices using the enzyme laccase combined with mathematical modelling." Thesis, University of Nottingham, 2017. http://eprints.nottingham.ac.uk/42868/.

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The presence and impact of steroid estrogens in natural water matrices has driven development and evaluation of wastewater treatment technologies that may reduce the steroid load entering water environments. This work was undertaken to assess and predict the ability of Trametes versicolor laccase to degrade estrone (E1) in water matrices under realistic conditions to wastewater treatment plants (WWTPs) and with consideration of the complex and variable nature of the wastewater matrix. A robust experimental procedure was developed to ensure the efficiency of the enzyme laccase to degrade E1 in
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15

Asmara, Widya. "Molecular biology of two 2-haloacid halidohydrolases." Thesis, University of Kent, 1991. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.293909.

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16

Zhou, Dao Min. "An investigation of some electrochemical characteristics of enzyme based disposable biosensors and other relevant electrodes." Thesis, University of Ulster, 1994. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.242072.

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17

Alarcon-Rojo, Alma D. "Changes in texture, structure and enzyme levels in conditioning of meat and their relationship with tenderisation." Thesis, University of Bristol, 1989. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.292512.

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18

Odnell, Anna. "Influencing anaerobic digestion early stage processes for increased biomethane production from different substrate components." Licentiate thesis, Linköpings universitet, Kemi, 2018. http://urn.kb.se/resolve?urn=urn:nbn:se:liu:diva-147721.

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Finding alternatives to petroleum-based energy sources is of interest since it could reduce the emissions of net carbon dioxide to the atmosphere by increasing the usage of renewable energy sources. To do so improvements are needed in the renewable energy production sector. Biogas production is of interest since the anaerobic digestion process can degrade many different biomolecules and is, contrary to e.g. bioethanol and biodiesel, not dependent on specific molecules. Thus, many wastes such as slaughterhouse waste, sludge from waste water treatment and lignocellulose residual material etc. ca
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19

López, Olvera Enrique Argenis. "Kinetic studies of carrier conjugated protease inhibitors." Thesis, Uppsala universitet, Institutionen för biologisk grundutbildning, 2019. http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-397114.

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Conjugates of soybean trypsin inhibitor (SBTI) and potato serine protease inhibitor (PSPI) immobilized on metal oxide particles of ~100nm diameter were prepared. Inhibition of trypsin hydrolysis of BAPA by these conjugates was measured and enzyme kinetics constants kcat, KM, kcat/KM and ki were determined. Metal oxide particles presented an inhibitory effect similar to that of a competitive inhibitor, noticed through the increase value of the K M constant. Furthermore, PSPI conjugates had the highest inhibition of trypsin, illustrated by the significantly higher value of KM relative to the val
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20

Besong, Jane. "Molecular insights into a putative potyvirus RNA encapsidation pathway and potyvirus particles as enzyme nano-carriers." Thesis, Bordeaux, 2016. http://www.theses.fr/2016BORD0078/document.

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La présente étude avait pour but d'identifier de nouvelles stratégies pour la présentation sélective d'enzymes à la surface de nanoparticules virales dans le but d’une application potentielle dans la technologie des biocapteurs ou des puces à protéines. Les potyvirus ont été choisis comme nanosupports modèles. Les Potyvirus, le genre le plus large de la famille des Potyviridae, la seconde plus grande famille de virus de plante, sont responsables de très graves pertes dans les cultures. Ils forment des capsides flexibles en forme de bâtonnet entourant une seule molécule d'ARN positif simple bri
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21

Hong, Eock Kee. "Analysis of the hollow fiber membrane reactor using immobilized enzyme with deactivation." Ohio : Ohio University, 1986. http://www.ohiolink.edu/etd/view.cgi?ohiou1183132380.

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22

Riahi, Esmaeil. "Effect of high pressure treatment on the kinetics of enzyme inactivation and microbial destruction in apple juice." Thesis, McGill University, 2003. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=84424.

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High pressure (HP) processing, a novel technology, has excellent potential for non-thermal preservation of apple juice, the largest consumed fruit juice in North America. The objective of this research was to evaluate the application of HP treatment for inactivation of enzymes and destruction of microorganisms in apple juice. HP inactivation kinetics of selected enzymes (amylase, pectin methyl esterase and polyphenol oxidase) and microorganisms [Leuconostoc mesenteroides, Pichia membranaefaciens and Zygosaccharomyces badii, Escherichia coli (29055) and Escherichia coli (O157:H7)] in app
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23

Semlitsch, Stefan. "Building blocks for polymer synthesis by enzymatic catalysis." Doctoral thesis, KTH, Industriell bioteknologi, 2017. http://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-212499.

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The search for alternatives to oil-based monomers has sparked interest for scientists to focus on the use of renewable resources for energy production, for the synthesis of polymeric materials and in other areas. With the use of renewable resources, scientists face new challenges to first isolate interesting molecules and then to process them. Enzymes are nature’s own powerful catalysts and display a variety of activities. They regulate important functions in life. They can also be used for chemical synthesis due to their efficiency, selectivity and mild reaction conditions. The selectivity of
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24

Bernal, Juárez Berenice. "Tecnología enzimática para la sacarificación de biomasas vegetales en solventes neotéricos = Enzyme technology for plant biomass saccharification in neoteric solvents." Doctoral thesis, Universidad de Murcia, 2013. http://hdl.handle.net/10803/120446.

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Objetivos El objetivo general de esta Tesis Doctoral se centró en la aplicación de la tecnología enzimática para el diseño y optimización de procesos integrados de sacarificación de celulosa mediante el uso de la tecnología de líquidos iónicos y reactores de membrana. Metodología Para llevar a cabo este objetivo se realizaron tests de actividad y estabilidad del derivado enzimático inmovilizado de estudio en diferentes líquidos iónicos (ILs). En los procesos de disolución, regeneración de celulosa y recuperación del IL, se emplearon diferentes etapas de operación (mezclado, filtración y destil
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25

Löfdahl, Per-Åke. "On bacterial formats in protein library technology." Doctoral thesis, KTH, Molekylär Bioteknologi, 2009. http://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-10993.

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Millions of years of evolution have resulted in an immense number of different proteins, which participate in virtually every process within cells and thus are of utmost importance for allknown forms of life. In addition, there are several examples of natural proteins which have found use in applications outside their natural environment, such as the use of enzymes infood industry and washing powders or the use of antibodies in diagnostic, bioseparation or therapeutic applications. To improve the performance of proteins in such applications, anumber of techniques, all collectively referred to
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26

Gullfot, Fredrika. "Synthesis of xyloglucan oligo- and polysaccharides with glycosynthase technology." Licentiate thesis, KTH, School of Biotechnology (BIO), 2009. http://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-10178.

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<p>Xyloglucans are polysaccharides found as storage polymers in seeds and tubers, and as cross-linking glycans in the cell wall of plants. Their structure is complex with intricate branching patterns, which contribute to the physical properties of the polysaccharide including its binding to and interaction with other glycans such as cellulose.</p><p>Xyloglucan is widely used in bulk quantities in the food, textile and paper making industries. With an increasing interest in technically more advanced applications of xyloglucan, such as novel biocomposites, there is a need to understand and contr
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27

Östling, Jeanette. "An Approach to Improve the Detection System of a Diagnostic Enzyme-Linked Immunosorbent Assay." Thesis, KTH, Skolan för bioteknologi (BIO), 2016. http://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-214620.

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28

Owen, Linda Mary Welsh. "Development of a liquid chromatography-inductively coupled plasma mass spectrometry method for zinc specification in an in vitro enzyme digest of cooked food." Thesis, Lancaster University, 1991. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.314443.

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29

Lacki, Karol M. "A novel method for the decrease of phenolic content in commercial canola meal using an enzyme preparation secreted by the white-rot fungus Trametes versicolor." Thesis, National Library of Canada = Bibliothèque nationale du Canada, 1997. http://www.collectionscanada.ca/obj/s4/f2/dsk2/ftp02/NQ28353.pdf.

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30

Leksawasdi, Noppol Biotechnology &amp Biomolecular Sciences (BABS) UNSW. "Kinetics and modelling of enzymatic process for R-phenylacetylcarbinol (PAC) production." Awarded by:University of New South Wales. Biotechnology and Biomolecular Sciences (BABS), 2004. http://handle.unsw.edu.au/1959.4/20846.

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R-phenylacetylcarbinol (PAC) is used as a precursor for production of ephedrine and pseudoephedrine, which are anti-asthmatics and nasal decongestants. PAC is produced from benzaldehyde and pyruvate mediated by pyruvate decarboxylase (PDC). A strain of Rhizopus javanicus was evaluated for its production of PDC. The morphology of R. javanicus was influenced by the degree of aeration/agitation. A relatively high specific PDC activity (328 U decarboxylase g-1 mycelium) was achieved when aeration/agitation were reduced significantly in the latter stages of cultivation. The stability of partially
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31

Basak, Sarmistha. "Studies on high pressure processing of orange juice : enzyme inactivation, microbial destruction, and quality changes, process verification and storage." Thesis, McGill University, 2001. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=36874.

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High pressure (HP) processing has been emerging rapidly as a novel technique for non-thermal preservation of foods. Application of HP processing for shelf life extension of orange juice was the principal objective of the current research. To accomplish this objective, and to establish a scientific basis for HP processing of orange juice, a systemic approach was used which included the evaluation of: (a) HP inactivation kinetics of pectin methyl esterase (PME, the key enzyme in orange juice implicated with respect to quality changes), (b) destruction of spoilage microorganisms and changes in pr
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32

Andersson, Charlotta. "Structural studies of Erwinia carotovora L-Asparaginase by X-ray crystallography." Thesis, Linköping University, The Department of Physics, Chemistry and Biology, 2006. http://urn.kb.se/resolve?urn=urn:nbn:se:liu:diva-6188.

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<p>Bacterial L-asparaginases (E.C.3.5.1.1) are enzymes that catalyze the hydrolysis of L-asparagine to aspartic acid. For the past 30 years these enzymes have been used as therapeutic agents in the treatment of acute childhood lymphoblastic leukemia. The presence of a low rate glutaminase activity however causes serious side-effects to patients in treatment, as glutamine depletion give rise to neurotoxicity, anaphylaxis, and other hypersensitivity reactions. The interest in the enzyme from Erwinia carotovora originates from the fact that it shows a decreased glutaminase activity, and therefore
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33

Moberg, Ylva. "Evaluation of an equine-optimized enzyme-linked immunosorbent assay for the determination of serum insulin in canine and feline samples." Thesis, Uppsala universitet, Institutionen för kvinnors och barns hälsa, 2018. http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-354036.

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Background: Insulin is an important hormone for glucose homeostasis. It is released from β-cells in the endocrine pancreas as a response to increased concentrations of plasma glucose. The major effect of insulin is the facilitation of cellular uptake and storage of glucose as glycogen. Insulinomas are tumours that produce excessive amounts of insulin resulting in hypoglycaemia. The condition has been observed in dogs and cats and is often malignant. One part of establishing the diagnosis is confirmation of elevated concentrations of insulin in a hypoglycaemic sample. Aim: The aim of this study
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34

Gashi, Krasniqi Lauresha. "Jämförelse av två enzyme-linked immunosorbent assays : mätning av diabetesspecifika autoantikroppar i en adult population." Thesis, Högskolan Kristianstad, Avdelningen för miljö- och biovetenskap, 2018. http://urn.kb.se/resolve?urn=urn:nbn:se:hkr:diva-18698.

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Typ- 1 diabetes (T1D) är en autoimmun sjukdom med insulinbrist orsakad av nedbrytning av insulinproducerande betaceller i pankreas. Fyra olika antikroppar har identifierats som är riktade mot betacellsspecifika antigen; insulinautoantikroppar (IAA), glutamic acid decarboxylase antibodies (GADA), islet antigen2-antikroppar (IA-2A) och antikroppar riktade mot zinktransportören 8 (ZnT8A). I denna studie gjordes en jämförelse av metoderna 2screen islet cell autoantibody ELISA-kit (RSR, Cardiff, UK) och 3screen islet cell autoantibody ELISA- kit (RSR, Cardiff, UK), vars brunnar är coatade med GAD65
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35

Liparulo, Irene <1991&gt. "Convergent bioenergetic defects in Coenzyme Q10 depleted cells by pharmacological inhibition of coq2 enzyme (p-hydroxybenzoate polyprenyl transferase) and by genome editing technology targeting the encoding gene (COQ2)." Doctoral thesis, Alma Mater Studiorum - Università di Bologna, 2021. http://amsdottorato.unibo.it/9756/7/Liparulo_Irene_tesi.pdf.

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Primary CoQ10 deficiency diseases encompass a heterogeneous spectrum of clinical phenotypes. Among these, defect or mutation on COQ2 gene, encoding a para-hydroxybenzoate polyprenyl transferase, have been associated with different diseases. Understanding the functional and metabolic impact of COQ2 mutation and the consequent CoQ10 deficiency is still a matter of debate. To date the aetiology of the neurological phenotypes correlated to CoQ10 deficiency does not present a clear genotype-phenotype association. In addition to the metabolic alterations due to Coenzyme Q depletion, the impairment o
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36

Kelly, Molly Kathleen. "Effect of Foliar Nitrogen and Sulfur Applications on Aroma Profile of Vitis vinifera L. cv. Petit Manseng using Modified Quantitative Descriptive Analysis, SPME GC-MS and Electronic Nose Technology." Diss., Virginia Tech, 2013. http://hdl.handle.net/10919/49536.

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Petit Manseng grapes harvested in 2011 and 2012 were fertilized with soil nitrogen at 0, and 30 kgN/ha, foliar nitrogen at 15kg/ha and foliar nitrogen plus sulfur at 15kg/ha and 5kg respectively. Point quadrat analysis demonstrated foliar nitrogen alone and nitrogen plus sulfur treatments increased percent gaps and lower leaf layer numbers. Berry juice samples differed in ammonia, arginine and yeast assimilable nitrogen concentration. Total glycosides were 25 percent higher in the foliar nitrogen treatment versus the control treatment. Electronic nose measurements on field clusters and laborat
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Nourizad, Nader. "Recombinant Enzymes in Pyrosequencing Technology." Doctoral thesis, KTH, Biotechnology, 2004. http://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-3765.

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<p>Pyrosequencing is a DNA sequencing method based on thedetection of released pyrophosphate (PPi) during DNA synthesis.In a cascade of enzymatic reactions, visible light isgenerated, which is proportional to the number of nucleotidesincorporated into the DNA template. When dNTP(s) areincorporated into the DNA template, inorganic PPi is released.The released PPi is converted to ATP by ATP sulfurylase, whichprovides the energy to luciferase to oxidize luciferin andgenerate light. The excess of dNTP(s) and the ATP produced areremoved by the nucleotide degrading enzyme apyrase.</p><p>The commerci
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Lim, Yuen Feung. "Optimisation of an enzyme treatment process for sheepskins : a thesis presented in partial fulfilment of the requirements for the degree of Master of Engineering in Bio-Process Engineering at Massey University (Manawatu), New Zealand." Massey University, 2009. http://hdl.handle.net/10179/1312.

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An enzyme treatment process for the pre-tanning of sheepskins has been previously reported by the Leather and Shoe Research Association of New Zealand (LASRA) as an alternative to current industry operations. The newly developed process had marked benefits over conventional processing in terms of a lowered energy usage (73%), processing time (47%) as well as water use (49%), but had been developed as a “proof of principle’’. The objective of this work was to develop the process further to a stage ready for adoption by industry. A process ready for adoption by industry is one that is able to ge
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Friberg, Andrew S. "Standardization of Islet Isolation and Transplantation Variables." Doctoral thesis, Uppsala universitet, Klinisk immunologi, 2011. http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-150247.

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Currently, the transplantation of islets of Langerhans is a viable means to maintain control of blood sugar levels and reduce the risk of hypoglycemia in defined populations with brittle type I diabetes mellitus or those requiring pancreatectomy. However, the process of islet isolation is highly variable and not all isolations result in islet numbers or quality suitable for transplantation. This thesis aimed to improve transplantation success through optimization and standardization of the isolation process and to identify pretransplant variables associated with early islet engraftment. A prev
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40

Håkansson, Hederos Sofia. "Catalysis and Site-Specific Modification of Glutathione Transferases Enabled by Rational Design." Doctoral thesis, Linköpings universitet, Organisk Kemi, 2005. http://urn.kb.se/resolve?urn=urn:nbn:se:liu:diva-3962.

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This thesis describes the rational design of a novel enzyme, a thiolester hydrolase, derived from human glutathione transferase (GST) A1-1 by the introduction of a single histidine residue. The first section of the thesis describes the design and the determination of the reaction mechanism. The design was based on the crystal structure of human GST A1-1 complexed with S-benzylglutathione. The resulting enzyme, A216H, catalyzed the hydrolysis of the non-natural substrate GSB, a thiolester of glutathione and benzoic acid. The reaction followed saturation kinetics with a kcat of 0.00078 min-1 and
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41

Mao, Yuhong [Verfasser], Ulrich [Akademischer Betreuer] Kulozik, Ulrich [Gutachter] Kulozik, and Wilfried [Gutachter] Schwab. "Immobilized enzyme technology for the production of whey protein hydrolysates : Design of flow-through trypsin reactors, influence of hydrolytic environment, and comparison with free trypsin / Yuhong Mao ; Gutachter: Ulrich Kulozik, Wilfried Schwab ; Betreuer: Ulrich Kulozik." München : Universitätsbibliothek der TU München, 2019. http://d-nb.info/1187443972/34.

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42

Edmonds, Richard. "Proteolytic depilation of lambskins : a thesis presented in partial fulfilment of the requirements for the degree of Doctor of philosophy in Bioprocess Engineering at Massey University, Palmerston North, New Zealand." Massey University, 2008. http://hdl.handle.net/10179/892.

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Abstract (sommario):
The processing of lambskins plays an important role in the New Zealand meat industry. The use of enzyme dewooling offers considerable advantages over the conventional depilation method which generates unpleasant working conditions and poses product quality risks when not properly handled. Prior to this work it was unclear from the literature why the practice of enzymatic depilation had not generally been adopted by industry. The aim of this work was to determine the problems associated with enzymatic depilation and provide a mechanistic understanding of the dewooling and damaging processes of
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43

Bøwadt, Thea. "Mitigating the impact of antidrug antibodies against insulin on ELISA assay." Thesis, Malmö universitet, Institutionen för biomedicinsk vetenskap (BMV), 2021. http://urn.kb.se/resolve?urn=urn:nbn:se:mau:diva-44473.

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Diabetes has, in the past three decades, surged immensely. Because of this, new insulin analogues are constantly in the making.  In clinical studies, the presence of antidrug antibodies can prove a challenge when measuring insulin. In order to overcome the interference from antidrug antibody complexes on the total insulin measurement in human serum, several pre-treatment methods on insulin and polyclonal antibodies spiked samples were tried using ELISA analysis. Several different methods were tried, acid dissociation using a glycine buffer with and without ethanol in different concentrations,
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44

Ayixiamuguli, Nueraimaiti. "Lignin degradation using lignolytic enzymes." Thesis, University of Nottingham, 2016. http://eprints.nottingham.ac.uk/35262/.

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Lignin is the only plant biomass that contains aromatic groups in its structure and can provide a wide range of low molecular weight aromatic chemicals if its depolymerisation can be achieved successfully. Currently, lignin is mainly produced as a waste by-product by the paper and pulp industry and biorefineries. Therefore, the transformation of the phenolic-rich lignin into value added aromatic platform chemicals can be regarded of primary concern to improve the economic profitability of biorefining. Moreover, being a renewable resource, the consumption of fossil fuels will be reduced if lign
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45

Koeth, Richard A. II. "The Green Functionalization and Crosslinking of Polyisobutylenes for Bioadhesive Employment." University of Akron / OhioLINK, 2012. http://rave.ohiolink.edu/etdc/view?acc_num=akron1353178867.

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46

Guedidi, Sadika. "Elaboration de membranes biocatalytiques par immobilisation d'enzyme dans des couches de polyélectrolytes." Montpellier 2, 2009. http://www.theses.fr/2009MON20195.

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Abstract (sommario):
L'objectif de ce travail a été d'étudier l'immobilisation d'enzymes sur des matériaux membranaires en utilisant la technique de dépôt multicouches de polyélectrolytes. Pour cela, nous avons utilisé deux enzymes de taille et de charge différentes, la trypsine et l'uréase, afin de mettre en évidence le rôle de la localisation des enzymes dans le matériau (modification de surface ou dans les pores). La stabilité au cours du temps du stockage a été étudié et a montré que l'immobilisation des enzymes dans des couches de polyélectrolytes améliore leur stabilité de façon très significative. Deux tech
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47

Souza, Monica Vinhas de. "Avaliação de tecnologias de saúde envolvendo doenças raras e tratamentos inovadores : Doença de Fabry e terapia de reposição enzimática." reponame:Biblioteca Digital de Teses e Dissertações da UFRGS, 2013. http://hdl.handle.net/10183/152861.

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Abstract (sommario):
As doenças raras são epidemiologicamente caracterizadas por ocorrerem entre 0,65-1:1.000 indivíduos. Houve importante incremento no surgimento de tratamentos específicos para muitas destas condições a partir da publicação de legislações específicas em diferentes países (sendo o pioneiro em 1983 nos EUA o chamado 'Orphan Drug Act'). Dentre as doenças que dispõem atualmente de tratamento específico está a Doença de Fabry, uma doença genética classificada erro inato do metabolismo do grupo das doenças Lísossômica se caracterizada pelo acúmulo de glicoesfíngol ipídíos no endotélio vascular, o que
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48

Towalski, Zbigniew. "The integration of knowledge within science, technology and industry : enzymes a case study." Thesis, Aston University, 1985. http://publications.aston.ac.uk/15166/.

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Abstract (sommario):
Enzyme technology is widely regarded as an exciting new technology possessing great opportunities for commercial interests and is one of a small group of key technologies singled out by the Science Research Councils during the 1960's as worthy of special support. In this thesis I outline the basic characteristics of this technology analysing the nature of the Government's policy towards it. The approach I have chosen requires an in depth analysis of the innovation process for enzymes which forms the basis for a model. This model is then used to focus on aspects of the UK science policy towards
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49

Hansen, Helena, and Suzanne Albinsson. "Enzym för att motverka fällningar av oligomerer : en jämförelse av hjälpkemikalien Sera Con P-NSI och enzymet cutinase NS59038 i färgningsprocessen för Trevira CS." Thesis, Högskolan i Borås, Akademin för textil, teknik och ekonomi, 2019. http://urn.kb.se/resolve?urn=urn:nbn:se:hb:diva-21960.

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Färgning av polyester (PET) sker i temperaturer omkring 130℃ och som en följd av den höga värmen migrerar oligomerer ut ur PET-fibern. Dessa oligomerer skapar därefter problem i form av vita fällningar och avlagringar, som leder till en reducering av maskinernas effektivitet, samt försämring av materialets utseende. En vanligt förekommande metod för att begränsa problemet är att tillsätta hjälpkemikalier i färgbadet. Novozymes A/S hävdar att genomförda studier med PET-garn och enzymet cutinase NS59038 har visat en minskning av det vita damm som kan ses på garnet i samband med migration av olig
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50

Mechmechani, Samah. "Hurdle technology using microencapsulated proteolytic enzymes and microencapsulated carvacrol to fight pathogenic bacterial biofilms." Electronic Thesis or Diss., Université de Lille (2022-....), 2022. http://www.theses.fr/2022ULILR023.

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L'environnement opératoire dans les domaines alimentaire et médical permet aux bactéries de se fixer et de se développer sur les surfaces, ce qui entraîne la formation de biofilms bactériens pathogènes et résistants. Ces structures pathogènes sont responsables de nombreuses maladies d'origine alimentaire et d'infections associées aux soins. Par conséquent, pour lutter contre ce fléau de santé publique, plusieurs stratégies ont récemment été proposées, notamment l'élimination chimique et mécanique. Ce travail présente les différents facteurs qui influencent l'adhésion bactérienne et la formatio
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