Bibliografie tematiche / Fluorescence

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Letteratura scientifica selezionata sul tema "Fluorescence"

Autore: Grafiati
Pubblicato: 4 giugno 2021
Ultima modifica: 25 luglio 2025

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Articoli di riviste sul tema "Fluorescence"

1

Sona, B. Warrier and Prashant S. Kharkar*. "DESIGN, SYNTHESIS AND CHARACTERIZATION OF FLUORESCENT PROBES BASED ON SCHIFF BASES." Indo American Journal of Pharmaceutical Sciences 04, no. 06 (2017): 1505–10. https://doi.org/10.5281/zenodo.815365.

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Abstract: Schiff bases of fluorescein were synthesized from fluorescein hydrazide using different aromatic aldehydes. These compounds show bright fluorescence different from that of starting material, fluorescein. The compounds were characterised by spectroscopic methods. Absorption and fluorescence spectra of the compounds were also recorded. Keywords: Fluorescein, Schiff bases, Fluorescein hydrazide, fluorescent probes, fluorescence
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MATSUMOTO, TAKURO, ATSUSHI SUETSUGU, KOSUKE HASEGAWA, et al. "A Mouse Model of Fluorescent Protein-expressing Disseminated Peritoneal Lymphoma for Fluorescence-guided Surgery." Anticancer Research 36, no. 9 (2016): 4483–88. http://dx.doi.org/10.21873/anticanres.10993.

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Pawley, James B. "Fluorescence Microscopy and Fluorescence Probes." Microscopy and Microanalysis 4, no. 2 (1998): 164–65. http://dx.doi.org/10.1017/s1431927698000166.

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Fluorescence Microscopy and Fluorescence Probes, edited by Jan Slavik, 1996. Plenum Press, New York and London. 306 pages. (hardback, $95)This volume is a compilation of abstracts of papers presented at the Fluorescence Microscopy and Fluorescence Probes Conference held in Prague in June 1995. This conference was one of the first tangible results of the end of the Cold War in the field of microscopy. As such, it attracted a great many of those who constitute the vanguard of progress in the field of fluorescence microscopy, on both sides of the old East-West divide. About one-third of the 127 c
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Sessions, S. K. "Fluorescence Microscopy; Quantitative Fluorescence Microscopy." Systematic Biology 42, no. 2 (1993): 224–25. http://dx.doi.org/10.1093/sysbio/42.2.224.

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Heldt, Józef, Janina R. Heldt, and Jerzy Kamiński. "Steady-state and Time-resolved Spectroscopic Studies of Benzanilides." Zeitschrift für Naturforschung A 54, no. 8-9 (1999): 495–502. http://dx.doi.org/10.1515/zna-1999-8-909.

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Steady-state and time-resolved spectroscopic studies of benzanilide (I) and jV-methylbenzanilide (II)were performed at 298 and 77 K in various solvents. The results indicate that benzanilide fluorescencein non-polar solvents at room temperature involves three independent modes of emission: F1 (LE) normalfluorescence from the initially excited state S1 (LE) with λmax = 320 nm, F2´(PT) fluorescence from the proton transfer tautomer with λmax = 468 nm, F2″CT) fluorescence from the species where intramolecular charge transfer appears, with λmax = 510 nm. At 77 K in MCH a new fluorescence band, Fag
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Ravinson, Daniel Sylvinson Muthiah, and Mark E. Thompson. "Thermally assisted delayed fluorescence (TADF): fluorescence delayed is fluorescence denied." Materials Horizons 7, no. 5 (2020): 1210–17. http://dx.doi.org/10.1039/d0mh00276c.

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Thermally assisted delayed fluorescence (TADF) allows for efficient collection of both singlet and triplet excitons with both emitting through the singlet channel. TADF opens the door to photo- and electroluminescence efficiencies close to 100%.
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Ma, Xiben, Jimeng Cheng, Sijun Fan, et al. "Silver-Neodymium Codoped Lithium Aluminum Metaphosphate Glasses for Radio-Photoluminescence Dosimeter." Materials 15, no. 16 (2022): 5527. http://dx.doi.org/10.3390/ma15165527.

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Commercial radio-photoluminescence (RPL) glass dosimeters generally use Ag single-doped phosphate glass as a single-wavelength sensor. Now, a novel type of Ag–Nd-codoped phosphate glass has been developed, which can be applied to dual-wavelength or multi-wavelength RPL sensors, and can thus improve the accuracy and stability of RPL dosimeters. An anhydrous 99.5 (0.7LiPO3–0.3Al (PO3)3) −0.25Ag2O–0.25Nd2O3 glass was prepared and irradiated at different doses, and then the absorption, fluorescence, infrared transmission spectra, as well as fluorescence lifetimes were tested and analyzed. The resu
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BEARDER, E. ARTHUR. "Fluorescence." Journal of the Society of Dyers and Colourists 27, no. 12 (2008): 270–79. http://dx.doi.org/10.1111/j.1478-4408.1911.tb00530.x.

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Inoue, S., O. Shimomura, M. Goda, M. Shribak, and P. T. Tran. "Fluorescence polarization of green fluorescence protein." Proceedings of the National Academy of Sciences 99, no. 7 (2002): 4272–77. http://dx.doi.org/10.1073/pnas.062065199.

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Gardner, Craig M., Steven L. Jacques, and Ashley J. Welch. "Fluorescence spectroscopy of tissue: recovery of intrinsic fluorescence from measured fluorescence." Applied Optics 35, no. 10 (1996): 1780. http://dx.doi.org/10.1364/ao.35.001780.

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Tesi sul tema "Fluorescence"

1

Nagl, Stefan. "Fluorescent multiple chemical sensing using time-domain fluorescence lifetime imaging." kostenfrei, 2008. http://www.opus-bayern.de/uni-regensburg/volltexte/2008/996/.

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Remy, Charlotte. "Synthèse et étude de récepteurs moléculaires fluorescents pour la détection de molécules neutres." Thesis, Université Paris-Saclay (ComUE), 2016. http://www.theses.fr/2016SACLN070/document.

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La détection de molécules toxiques pour l’Homme et son environnement est d’une importance cruciale et fait partie des préoccupations majeures de la société actuelle. Les résidus de pesticides tels que l’atrazine ainsi que la mélamine font notamment partie de ces molécules dangereuses pour la santé humaine. Ces deux molécules sont principalement dosées par des techniques lourdes et coûteuses comme la spectrométrie de masse, la chromatographie ou l’électrochimie. De même, la détection des amines biogéniques représente un intérêt sociétal. Elles sont produites par des bactéries durant la décarbox
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Dennis, Allison Marie. "Quantum dot-fluorescent protein pairs as fluorescence resonance energy transfer pairs." Diss., Georgia Institute of Technology, 2009. http://hdl.handle.net/1853/37079.

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Fluorescence resonance energy transfer (FRET)-based biosensors have been designed to fluorometrically detect everything from proteolytic activity to receptor-ligand interactions and structural changes in proteins. While a wide variety of fluorophores have demonstrated effectiveness in FRET probes, several potential sensor components are particularly notable. Semiconductor quantum dots (QDs) are attractive FRET donors because they are rather bright, exhibit high quantum yields, and their nanoparticulate structure enables the attachment of multiple acceptor molecules. Fluorescent proteins (FPs)
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M'Baye, Gora Duportail Guy. "Sondes fluorescentes ratiométriques dérivées de la 3- Hydroxyflavone Etude spectroscopique de nouveaux dérivés et applications en biophysique membranaire /." Strasbourg : Université Louis Pasteur, 2007. http://eprints-scd-ulp.u-strasbg.fr:8080/755/01/MBAYE2007.pdf.

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Henderson, Julius Nathan. "Crystallographic and spectroscopic studies of photoswitching in fluorescent proteins /." view abstract or download file of text, 2007. http://proquest.umi.com/pqdweb?did=1417810431&sid=5&Fmt=2&clientId=11238&RQT=309&VName=PQD.

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Thesis (Ph. D.)--University of Oregon, 2007.<br>Typescript. Includes vita and abstract. Includes bibliographical references (leaves 143-151). Also available for download via the World Wide Web; free to University of Oregon users.
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Boujut, Margot. "Ligands Photo-Actifs pour l'imagerie de fluorescence du VEGFr." Thesis, Normandie, 2020. http://www.theses.fr/2020NORMR063.

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Abstract (sommario):
Les VEGFr (Vascular Endothelium Growth Factor receptors, récepteurs des facteurs de croissance de l’endothélium vasculaire) sont des protéines responsables de l’angiogenèse, c’està-dire, la croissance des vaisseaux sanguins. De fait, ils sont impliqués dans les maladies liées à une vascularisation néfaste comme dans la croissance des tumeurs cancéreuses ou de néovascularisation rétinienne. Traiter ces vaisseaux sanguins sans atteindre les tissus sains est un enjeu qui nécessite de les imager spécifiquement et précisément, ces deux critères pouvant être atteints par imagerie de fluorescence. La
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Goulas, Yves. "Teledetection de la fluorescence des couverts vegetaux : temps de vie de la fluorescence chlorophyllienne et fluorescence bleue." Paris 11, 1992. http://www.theses.fr/1992PA112264.

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La mesure de la fluorescence de la chlorophylle in vivo est une methode d'investigation largement utilisee pour le suivi de l'activite photosynthetique, sur algues, chlorophastes ou feuilles entieres. Sur un couvert vegetal, la mesure a distance de la duree de vie de fluorescence permet de determiner directement le rendement quantique et peut ainsi etre envisagee comme methode de teledetection de l'etat physiologique de la vegetation. Dans cette perspective, nous avons etudie les possibilites qu'offre la fluorescence resolue en temps. Nous avons etudie l'effet du quenching energetique sur les
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Cheng, Hok Yan. "Near infrared fluorescence probes : towards applications in fluorescence guided surgery." Thesis, University of Hull, 2017. http://hydra.hull.ac.uk/resources/hull:16529.

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Surgery has been a popular method for the treatment of cancers, in particular solid tumours; but the surgical margins for cancerous tissues are often indistinct and in most cases, the poor identification of residual cancer tissues can result in re-excision. Therefore, near infrared (NIR) fluorescence-guided surgery (FGS) is being developed as a real time intra-operative imaging technique to assist surgeons by improving the accuracy and precision of the removal of tumours. However, current FDA approved fluorophores suffer from poor chemical stability, limited water-solubility, and lack selectiv
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Zhou, Xiaobo. "Design, synthesis and sensing properties of chiral amine-based fluorescent probes." HKBU Institutional Repository, 2012. https://repository.hkbu.edu.hk/etd_ra/1442.

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Aknine, Nathan. "Nouvelles sondes fluorescentes pour la bioimagerie des structures lipidiques cellulaires." Electronic Thesis or Diss., Strasbourg, 2024. http://www.theses.fr/2024STRAF035.

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Ce projet de doctorat vise la conception de sondes fluorescentes innovantes, pour une meilleure visualisation et compréhension des structures lipidiques cellulaires par bioimagerie. Ainsi, trois familles de nouvelles sondes fluorescentes ont été générées dans le cadre de cette thèse, visant à cibler et éclairer différentes localisations cellulaires. Premièrement, le ciblage covalent de la membrane plasmique par une stratégie nommée MemGraft, a permis de développer de nouveaux outils pour le marquage et la fonctionnalisation de la surface cellulaire. Ces sondes permettent un marquage robuste et
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Libri sul tema "Fluorescence"

1

Slavík, Jan, ed. Fluorescence Microscopy and Fluorescent Probes. Springer US, 1996. http://dx.doi.org/10.1007/978-1-4899-1866-6.

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D, Slavík Jan Ph, and Conference on Fluorescence Microscopy and Fluorescent Probes (1995 : Prague, Czech Republic), eds. Fluorescence microscopy and fluorescent probes. Plenum Press, 1996.

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Deatrick, Elizabeth. Fluorescence. The author, 2017.

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B, Thompson Richard, ed. Fluorescence sensors and biosensors. Taylor&Francis, 2005.

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Kubitscheck, Ulrich, ed. Fluorescence Microscopy. Wiley-VCH Verlag GmbH & Co. KGaA, 2017. http://dx.doi.org/10.1002/9783527687732.

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Kubitscheck, Ulrich, ed. Fluorescence Microscopy. Wiley-VCH Verlag GmbH & Co. KGaA, 2013. http://dx.doi.org/10.1002/9783527671595.

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Wolfbeis, Otto S., ed. Fluorescence Spectroscopy. Springer Berlin Heidelberg, 1993. http://dx.doi.org/10.1007/978-3-642-77372-3.

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Valeur, Bernard, and Mário Nuno Berberan-Santos. Molecular Fluorescence. Wiley-VCH Verlag GmbH & Co. KGaA, 2012. http://dx.doi.org/10.1002/9783527650002.

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Herman, B. Fluorescence microscopy. 2nd ed. BIOS, 1998.

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Dick, Jennifer K. Fluorescence: Poems. University of Georgia Press, 2004.

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Capitoli di libri sul tema "Fluorescence"

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Gerritsen, Hans C. "Confocal Fluorescence Lifetime Imaging." In Fluorescence Microscopy and Fluorescent Probes. Springer US, 1996. http://dx.doi.org/10.1007/978-1-4899-1866-6_3.

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Cimprich, Petr, and Jan Slavík. "Artifacts in Fluorescence Ratio Imaging." In Fluorescence Microscopy and Fluorescent Probes. Springer US, 1996. http://dx.doi.org/10.1007/978-1-4899-1866-6_16.

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O’Connor, José-Enrique. "Flow Cytometry versus Fluorescence Microscopy." In Fluorescence Microscopy and Fluorescent Probes. Springer US, 1996. http://dx.doi.org/10.1007/978-1-4899-1866-6_6.

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Gundlach, Heinz. "Multichannel Fluorescence Microscopy and Digital Imaging - On the Exciting Developments in Fluorescence Microscopy." In Fluorescence Microscopy and Fluorescent Probes. Springer US, 1996. http://dx.doi.org/10.1007/978-1-4899-1866-6_7.

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Herman, Brian. "Fluorescence Microscopy: State of the Art." In Fluorescence Microscopy and Fluorescent Probes. Springer US, 1996. http://dx.doi.org/10.1007/978-1-4899-1866-6_1.

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Tengholm, Anders, and Eva Grapengiesser. "Disappearance of Cytoplasmic Ca2+ Oscillations is a Sensitive Indicator of Photodamage in Pancreatic β-Cells." In Fluorescence Microscopy and Fluorescent Probes. Springer US, 1996. http://dx.doi.org/10.1007/978-1-4899-1866-6_10.

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Cimprich, Petr, and Jan Slavík. "Distribution of Individual Cytoplasmic pH Values in a Cell Suspension." In Fluorescence Microscopy and Fluorescent Probes. Springer US, 1996. http://dx.doi.org/10.1007/978-1-4899-1866-6_11.

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Gregor, Martin, Jan Tachezy, and Jan Slavík. "The Effect of Lysosomal pH on Lactoferrin-Dependent Iron Uptake in Tritrichomonas foetus." In Fluorescence Microscopy and Fluorescent Probes. Springer US, 1996. http://dx.doi.org/10.1007/978-1-4899-1866-6_12.

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Opitz, N., T. Porwol, E. Merten, and H. Acker. "On the Protein-Error of the Calcium-Sensitive Fluorescent Indicator Fura-Red." In Fluorescence Microscopy and Fluorescent Probes. Springer US, 1996. http://dx.doi.org/10.1007/978-1-4899-1866-6_13.

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Opitz, N., T. Porwol, E. Merten, and H. Acker. "Cytoplasmic Ion Imaging: Evidence for Intracellular Calibration Heterogeneities of Ion-Sensitive Fluoroprobes." In Fluorescence Microscopy and Fluorescent Probes. Springer US, 1996. http://dx.doi.org/10.1007/978-1-4899-1866-6_14.

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Atti di convegni sul tema "Fluorescence"

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Johnson, Richard E., and Vinod S. Agarwala. "Fluorescence Based Chemical Sensors for Corrosion Detection." In CORROSION 1997. NACE International, 1997. https://doi.org/10.5006/c1997-97304.

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Abstract Several fluorescent materials have been identified as possible corrosion sensing coatings. These are either redox or metal ion complex materials. The redox materials are nonfluorescent in the reduced state and become fluorescent upon oxidation. Incorporated into paint coatings, they provide an early warning of corrosive conditions at the metal or alloy surface. The metal ion complex materials only fluoresce when the organic compound complexes with metal ions such as those generated in corrosion reactions. Fluorescent materials have been incorporated into paint coatings and on metal su
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Scorzo, Augustino V., Caleb Y. Kwon, Rendall R. Strawbridge, and Scott C. Davis. "Comparison of a fast-acting contrast agent for fluorescence-guided neurosurgery with co-administered ALA-PpIX and second-window ICG using fluorescence cryotomography." In Optical Molecular Probes, Imaging and Drug Delivery. Optica Publishing Group, 2025. https://doi.org/10.1364/omp.2025.ow2e.1.

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A promising new untargeted, fast-acting and persistent contrast agent (TMR-PEG1k) for fluorescence-guided surgery was evaluated alongside two clinically tested fluorescent contrast agents for glioma resection (ALA-PpIX and second-window ICG) using fluorescence cryotomography of whole mice.
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Abbey, Emma, Oren Katz, Travis Ferguson, Sarah-Johanna Klose, Chris Pruefert, and Hans-Peter Loock. "Applications of Hadamard-Multiplexed Fluorescence Imaging using a Hyperspectral Camera." In CLEO: Applications and Technology. Optica Publishing Group, 2024. http://dx.doi.org/10.1364/cleo_at.2024.am4a.2.

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We present a hyperspectral fluorescence imaging system based on a Hadamard-multiplexed light source for monitoring spatially inhomogeneous reactions through time and correctly identifying fluorescent components in complex mixtures.
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Bradley, Mark. "Fluorescence and fluorescent life imaging probes for biomedical applications." In Reporters, Contrast Agents, and Molecular Probes for Biomedical Applications XVI, edited by Ramesh Raghavachari and Mikhail Y. Berezin. SPIE, 2025. https://doi.org/10.1117/12.3049934.

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Durrett, Michael G., Richard E. Johnson, and Vinod S. Agarwala. "ICI, Intelligent Corrosion Indicator, and Its Use for the Early Detection of Corrosion on Aluminum Alloy Surfaces by Fluorescence." In CORROSION 2000. NACE International, 2000. https://doi.org/10.5006/c2000-00285.

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Abstract Several fluorescent materials have been used under epoxy coatings on 7075 aluminum alloy surfaces. These fluorescent materials either fluoresce upon oxidation and are thus corrosivity indicators or they fluoresce with the products of corrosion and are true corrosion indicators. Recent work has involved using a pigmented epoxy paint, such as that used on aircraft, to see if fluorescence is still visible. Although the intensity is reduced, there is still visible fluorescence. This paper reports the results of the most promising fluorescent markers in each of the two types mentioned abov
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Schreiter, Eric. "Chemigenetic Fluorescent Biosensors." In Optics and the Brain. Optica Publishing Group, 2024. https://doi.org/10.1364/brain.2024.bs1c.2.

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Abstract (sommario):
We have developed chemigenetic fluorescent indicators built from engineered protein sensor domains and bright fluorescent dyes. Advantages include increased photon output for demanding microscopy applications and bright fluorescence at wavelengths not well covered by fluorescent proteins. Full-text article not available; see video presentation
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Panova, Anna A., Paul Pantano, and David R. Walt. "In Situ Fluorescence Imaging of Localized Corrosion with a pH-Sensitive Imaging Fiber." In CORROSION 1997. NACE International, 1997. https://doi.org/10.5006/c1997-97314.

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Abstract A fiber optic pH-sensor capable of both visualizing corrosion sites and measuring local chemical concentrations is applied to real-time corrosion monitoring. The imaging fiber’s distal face containing an immobilized pH-sensitive fluorescent dye is brought into contact with metal surfaces submerged in aqueous buffers and fluorescence images are acquired as a function of time. The observed changes in fluorescence due to increases in pH at cathodic sites and decreases in pH at anodic sites are indicative of localized corrosion rates.
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Armstrong, R. L., J. G. Xie, T. E. Ruskgauer, and R. G. Pinnick. "Energy transfer lasing from dye-doped microdroplets seeded with fluorescent sol." In OSA Annual Meeting. Optica Publishing Group, 1992. http://dx.doi.org/10.1364/oam.1992.tug2.

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We report observations of lasing and fluorescence emission from liquid microdroplets of fluorescein 548 dye in ethanol, seeded with submicrometer sized fluorescent sol. An incident pump laser generates droplet fluo­rescein lasing or fluorescene, which in turn excites either fluorescene or lasing in the sol. All emissions are at wavelengths corresponding to morphology dependent reso­nances (MDR's) of the droplet. Studies of the dependence of these emissions on pump laser intensity and sol concentration suggest they are driven by enhanced radiative energy transfer that occurs when fluorescein la
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Maruoka, Shoji, Yohei Mitsui, Shinpei Okawa, Yoko Hoshi, and Yukio Yamada. "Measurement of Fluorescence Properties in Light Scattering Medium." In ASME/JSME 2011 8th Thermal Engineering Joint Conference. ASMEDC, 2011. http://dx.doi.org/10.1115/ajtec2011-44458.

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The purpose of this study is to measure the fluorescence properties of Indocyaninegreen (ICG) which is a fluorescence dye to be used as a fluorescence probe for the use of fluorescence imaging in biomedical applications. The fluorescence molecular imaging is expected to solve the issues in preclinical studies which require a lot of time, labors and sacrificed animals. Information of living body can be obtained by measuring the fluorescent properties of the probe in biological media. The absorption and emission spectra and the lifetime of ICG in non-scattering and scattering media were measured
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Deliolanis, Nikolaos C., Thomas Wurdinger, Bakhos Tannous, and Vasilis Ntziachristos. "Fluorescence Tomography of Red-shifted Fluorescent Proteins." In Biomedical Optics. OSA, 2010. http://dx.doi.org/10.1364/biomed.2010.btud4.

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Rapporti di organizzazioni sul tema "Fluorescence"

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Hargis, P. J. Jr, B. L. Preppernau, and B. P. Aragon. Ultraviolet fluorescence monitor. Office of Scientific and Technical Information (OSTI), 1997. http://dx.doi.org/10.2172/481480.

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Allison, S. W. Fluorescence Rise Time Measurements for High Temperature Fluorescence-Based Thermometry. Office of Scientific and Technical Information (OSTI), 2005. http://dx.doi.org/10.2172/885970.

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DeRose, Paul C. Standard guide to fluorescence :. National Institute of Standards and Technology, 2007. http://dx.doi.org/10.6028/nist.ir.7458.

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Haugland, R. P. Fluorescence-detected DNA sequencing. Office of Scientific and Technical Information (OSTI), 1990. http://dx.doi.org/10.2172/5619036.

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Deng, Chun, Zhenyu Zhang, Zhi Guo, et al. Assessment of intraoperative use of indocyanine green fluorescence imaging on the number of lymph node dissection during minimally invasive gastrectomy: a systematic review and meta-analysis. INPLASY - International Platform of Registered Systematic Review and Meta-analysis Protocols, 2021. http://dx.doi.org/10.37766/inplasy2021.11.0062.

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Review question / Objective: Whether is indocyanine green fluorescence imaging-guided lymphadenectomy feasible to improve the number of lymph node dissections during radical gastrectomy in patients with gastric cancer undergoing curative resection? Condition being studied: Gastric cancer was the sixth most common malignant tumor and the fourth leading cause of cancer-related death in the world. Radical lymphadenectomy was a standard procedure in radical gastrectomy for gastric cancer. The retrieval of more lymph nodes was beneficial for improving the accuracy of tumor staging and the long-term
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Mathies, R. A., and A. N. Glazer. Ultrasensitive fluorescence detection of DNA. Office of Scientific and Technical Information (OSTI), 1992. http://dx.doi.org/10.2172/7176600.

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Mathies, R. A., and A. N. Glazer. Ultrasensitive fluorescence detection of DNA. Office of Scientific and Technical Information (OSTI), 1991. http://dx.doi.org/10.2172/5827344.

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8

Guo, Yixing. Fluorescence Detection of Biological Thiols. Portland State University Library, 2000. http://dx.doi.org/10.15760/etd.586.

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ภุมมางกูร, สุนิพนธ์, ราณี สุรกาญจน์กุล та ลาวัลย์ ศรีพงษ์. การตรวจสอบเภสัชภัณฑ์โดยใช้ฟลูโอเรสคามีน : รายงานผลการวิจัย. จุฬาลงกรณ์มหาวิทยาลัย, 1989. https://doi.org/10.58837/chula.res.1989.10.

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Abstract (sommario):
การใช้ fluorescamine เป็น spraying reagent พ่นบน plate ที่เคลือบด้วย silica gel GF 254 สามารถใช้ตรวจสอบยาที่มีหมู่ฟังก์ชัน –NH2, -NH, และ –N- ได้ พบว่ายาที่เป็น primary amine จะเกิด fluorescence กับ fluorescamine แต่ยาที่เป็น secondary amine จะเกิด quenching ส่วนที่เป็น tertiary amine หรือ quarternary amine จะไม่เกิดอะไรกับ fluorescamine โดยอาศัยผลการทดลองอันนี้จะทำให้สามารถแบ่งยาเป็น 3 กลุ่ม คือกลุ่มที่ 1 เป็นยาที่เกิด fluorescence กับ fluorescamine กลุ่มที่ 2 เกิด quenching และกลุ่มที่ 3 ไม่เกิด fluorescence หรือ quenching กับ fluorescamine โดยอาศัยการแบ่งยาเป็นสามกลุ่มและการใช้ข้อมูลจาก Rf
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Scalettar, Bethe A. Fluorescence spectroscopic studies of DNA dynamics. Office of Scientific and Technical Information (OSTI), 1987. http://dx.doi.org/10.2172/10150990.

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