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Articoli di riviste sul tema "INS"

Autore: Grafiati

Pubblicato: 4 giugno 2021

Ultima modifica: 25 luglio 2025

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1

Muddasani, Anushareddy, Rachel Hendrix, Milan Bimali, and Asis Shrestha. "Intranasal Corticosteroid Use and Risk of Invasive Fungal Sinusitis in Acute Myeloid Leukemia, Myelodysplastic Syndrome and Post Transplant Patients." Blood 144, Supplement 1 (2024): 4232. https://doi.org/10.1182/blood-2024-193965.

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Background: Invasive fungal sinusitis (IFS) is a severe and often fatal infection with a mortality rate ranging from 50% to 80%, and primarily affects immunocompromised individuals. Previous research has identified several risk factors for IFS, including myeloid malignancies, allogeneic hematopoietic stem cell transplants (HSCTs), graft-vs-host disease (GVHD), delayed white blood cell (WBC) engraftment, and lymphopenia. Additionally, systemic steroid use has been associated with an increased risk of IFS. However, the impact of intranasal steroids (INS)-used locally rather than systemically-on

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2

Bitar, K. N., C. Hillemeier, P. Biancani, and K. J. Balazovich. "Regulation of smooth muscle contraction in rabbit internal anal sphincter by protein kinase C and Ins(1,4,5)P3." American Journal of Physiology-Gastrointestinal and Liver Physiology 260, no. 4 (1991): G537—G542. http://dx.doi.org/10.1152/ajpgi.1991.260.4.g537.

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We have examined the role of protein kinase C (PKC)-beta II and its functional relationship to inositol 1,4,5-trisphosphate [Ins(1,4,5)P3] and intracellular Ca2+ in the contraction of smooth muscle cells from the rabbit internal and sphincter (IAS). PKC-beta (0.1-100 U/ml) and Ins(1,4,5)P3 (10(-9) to 10(-6) M) caused concentration-dependent contraction of IAS smooth muscle cells permeabilized by saponin. The combination of threshold concentrations of Ins(1,4,5)P3 (10(-9) M) and PKC (0.1 U/ml) was more than additive, causing near maximal shortening (28.2 +/- 2.1% decrease in cell length from co

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Barkun, A., K. Ravanbakhsh, D. Kim, et al. "A72 THE ENDOSCOPIC YIELD OF PRIORITIZED INDICATIONS USING A PROVINCIAL-WIDE COLONOSCOPY REFERRAL FORM." Journal of the Canadian Association of Gastroenterology 7, Supplement_1 (2024): 49–50. http://dx.doi.org/10.1093/jcag/gwad061.072.

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Abstract Background The widespread use of a standardized and validated province-wide colonoscopy referral form (PCRF), regrouping mutually exclusive indications into suggested priority wait time categories, has allowed for a more comprehensive assessment of routine colonoscopy practice Aims To validate the Quebec PCRF by better characterizing endoscopic finding yields according to specific clinical indications. Methods This retrospective cohort study includes consecutive adult patients with available data from the PCRF from two tertiary hospitals. The primary outcome was the diagnostic rates o

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Trevisan, Gustavo Aléssio, Everton Leonardi da Silva, Anderson Luiz de Carvalho, and Rafael Messias Luiz. "EFEITOS ANESTÉSICOS DA ADMINISTRAÇÃO INTRANASAL OU INTRAMUSCULAR DA ASSOCIAÇÃO DE MIDAZOLAM E CETAMINA RACÊMICA OU S+ EM PERIQUITO AUSTRALIANO (Melopsittacus undulatus)." Ciência Animal Brasileira 17, no. 1 (2016): 126–32. http://dx.doi.org/10.1590/1089-6891v17i131271.

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Resumo A anestesia intranasal em aves é considerada uma técnica anestésica segura, simples e eficiente. O objetivo deste estudo foi comparar os efeitos anestésicos da associação de midazolam (5 mg.kg-1) e cetamina (15 mg.kg-1) nas formulações racêmica (R) ou S+ (S) administrados pela via intranasal (IN) ou intramuscular (IM) em periquitos australianos (Melopsittacus undulatus). Foram utilizados oito periquitos em delineamento do tipo crossover, em quatro tratamentos: INR, INS, IMR e IMS. Foram avaliados os tempos de latência, decúbito dorsal, anestesia e recuperação, grau de sedação e qualidad

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A.V, Raveendran. "Inhalational Steroids and Iatrogenic Cushing’s Syndrome." Open Respiratory Medicine Journal 8, no. 1 (2014): 74–84. http://dx.doi.org/10.2174/1874306401408010074.

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Bronchial asthma (BA) and Allergic rhinitis (AR) are common clinical problems encountered in day to day practice, where inhalational corticosteroids (ICS) or intranasal steroids (INS) are the mainstay of treatment. Iatrogenic Cushing syndrome (CS) is a well known complication of systemic steroid administration. ICS /INS were earlier thought to be safe, but now more and more number of case reports of Iatrogenic Cushing syndrome have been reported, especially in those who are taking cytochrome P450 (CYP 450) inhibitors. Comparing to the classical clinical features of spontaneous Cushing syndrome

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Fossataro, Claudia, Pia Clara Pafundi, Roberta Mattei, Valentina Cima, Francesca De Rossi, and Gustavo Savino. "Infantile nystagmus syndrome: An observational, retrospective, multicenter study." Optometry and Vision Science 101, no. 4 (2024): 211–23. http://dx.doi.org/10.1097/opx.0000000000002131.

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SIGNIFICANCE This multicenter study assessed clinical and psychological aspects of infantile nystagmus syndrome (INS) focusing on its management and nonsurgical treatment. PURPOSE This study aimed to assess clinical features, management, relationship life, and psychological impact in a group of patients with nystagmus onset in pediatric age. METHODS This observational study included patients diagnosed with INS referred to two Italian centers from January 1, 2017, to December 31, 2020. Ophthalmologic and orthoptic features and impact of visual function on quality of life, according to nystagmus

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7

Veuthey, Tania, Jeremy T. Florman, Sebastián Giunti, et al. "The neurohormone tyramine stimulates the secretion of an insulin-like peptide from the Caenorhabditis elegans intestine to modulate the systemic stress response." PLOS Biology 23, no. 1 (2025): e3002997. https://doi.org/10.1371/journal.pbio.3002997.

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The DAF-2/insulin/insulin-like growth factor signaling (IIS) pathway plays an evolutionarily conserved role in regulating reproductive development, life span, and stress resistance. In Caenorhabditis elegans, DAF-2/IIS signaling is modulated by an extensive array of insulin-like peptides (ILPs) with diverse spatial and temporal expression patterns. However, the release dynamics and specific functions of these ILPs in adapting to different environmental conditions remain poorly understood. Here, we show that the ILP, insulin-3 (INS-3), plays a crucial role in modulating the response to various

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8

Kleespies, Matthias Winfried, Tina Braun, Paul Wilhelm Dierkes, and Volker Wenzel. "Measuring Connection to Nature—A Illustrated Extension of the Inclusion of Nature in Self Scale." Sustainability 13, no. 4 (2021): 1761. http://dx.doi.org/10.3390/su13041761.

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The human-nature connection is an important factor that is frequently the subject of environmental education research and environmental psychology. Therefore, over the years, numerous measuring instruments have been established to quantitatively record a person’s connection to nature. However, there is no instrument specifically for children with cognitive limitations. For this reason, in this study, an established scale for connection to nature, the inclusion of nature in self scale (INS), was modified especially for the needs of this group. Study 1 investigated what students understand by th

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Timofeev, E. A., and G. S. Tsekhanovich. "INS simulator for debugging INS/GNSS data." IEEE Aerospace and Electronic Systems Magazine 24, no. 1 (2009): 38–40. http://dx.doi.org/10.1109/maes.2009.4772753.

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10

Azhermacheva, M., E. Therskih, K. Burkova, and D. Plotnikov. "Endothelial dysfunction in smoker patients/INS; with/INS; acute isc/INS;hemic stroke." Journal of the Neurological Sciences 333 (October 2013): e272. http://dx.doi.org/10.1016/j.jns.2013.07.1038.

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Haenicke, Diether H., and Helena Janeczek. "Ins Freie." World Literature Today 64, no. 4 (1990): 632. http://dx.doi.org/10.2307/40146936.

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Papachristopoulos, Ioannis. ""Ins offene..."." Die Musikforschung 61, no. 4 (2021): 349–67. http://dx.doi.org/10.52412/mf.2008.h4.510.

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Bernhart, Wolfgang, and Norbert Dressler. "Ins Grüne." Automotive Agenda 3, no. 3 (2010): 52–56. http://dx.doi.org/10.1007/bf03223782.

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Nissen, Madeleine. "Ins Unbekannte." Lebensmittel Zeitung 76, no. 40 (2024): 23–24. http://dx.doi.org/10.51202/0947-7527-2024-40-023.

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Martin, Gerhard Marcel. "Ins Offene." Praktische Theologie 40, no. 1 (2005): 73–76. http://dx.doi.org/10.14315/prth-2005-0118.

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Coulehan, Jack. "Shut-ins." JAMA 295, no. 19 (2006): 2225. http://dx.doi.org/10.1001/jama.295.19.2225.

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Finazzi-Agrò, Alessandro. "Prote-ins." Biotechnology and Applied Biochemistry 65, no. 1 (2018): 5–6. http://dx.doi.org/10.1002/bab.1639.

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18

Parin, Alexei. "INS INNERSTE." Opernwelt 64, no. 1 (2023): 38–39. http://dx.doi.org/10.5771/0030-3690-2023-1-038.

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Mattenklott, Gert. "«Komm ins Offene, Freund!» Transit ins wilde Denken." Zeitschrift für Ideengeschichte 2, no. 4 (2008): 5–10. http://dx.doi.org/10.17104/1863-8937-2008-4-5.

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Aurangzeb, S., and Z. Zaiwalla. "Paediatric narcolepsy — /INS;A seven-/INS;year experience." Journal of the Neurological Sciences 333 (October 2013): e687. http://dx.doi.org/10.1016/j.jns.2013.07.2373.

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Kovalyuk, Z. D. "Investigation of InS-InSe heterojunctions prepared using sulphurization of p-InSe." Semiconductor Physics Quantum Electronics and Optoelectronics 15, no. 1 (2012): 38–40. http://dx.doi.org/10.15407/spqeo15.01.038.

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YONESHIMA, Hiroyuki, Atsushi MIYAWAKI, Takayuki MICHIKAWA, Teiichi FURUICHI, and Katsuhiko MIKOSHIBA. "Ca2+ differentially regulates the ligand-affinity states of type 1 and type 3 inositol 1,4,5-trisphosphate receptors." Biochemical Journal 322, no. 2 (1997): 591–96. http://dx.doi.org/10.1042/bj3220591.

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To elucidate the functional difference between type 1 and type 3 Ins(1,4,5)P3 receptors [Ins(1,4,5)P3R1 and Ins(1,4,5)P3R3 respectively] we studied the effect of Ca2+ on the ligand-binding properties of both Ins(1,4,5)P3R types. We expressed full-length human Ins(1,4,5)P3R1 and Ins(1,4,5)P3R3 from cDNA species in insect ovary Sf9 cells, and the membrane fractions were used for Ins(1,4,5)P3-binding assays. The binding of Ins(1,4,5)P3 to Ins(1,4,5)P3R1 and Ins(1,4,5)P3R3 was differentially regulated by Ca2+. With increasing concentrations of free Ca2+ ([Ca2+]), Ins(1,4,5)P3 binding to Ins(1,4,5)

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Astriani, Nadia, and Yulinda Adharani. "PEMBANGUNAN KERETA CEPAT JAKARTA-BANDUNG DARI SUDUT PANDANG PENEGAKAN HUKUM PENATAAN RUANG." Jurnal Rechts Vinding: Media Pembinaan Hukum Nasional 6, no. 2 (2017): 243. http://dx.doi.org/10.33331/rechtsvinding.v6i2.173.

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<p>Pembangunan infrastruktur harus dilakukan dengan mempertimbangkan pembangunan berkelanjutan serta mengedepankan instrumen pencegahan dan/atau kerusakan lingkungan<ins cite="mailto:Apri%20Listiyanto" datetime="2017-08-08T09:37">, hal ini berlaku pula terhadap </ins><ins cite="mailto:Apri%20Listiyanto" datetime="2017-08-08T09:38">proyek </ins><ins cite="mailto:alice%20angelica" datetime="2017-08-18T15:16">i</ins><ins cite="mailto:Apri%20Listiyanto" datetime="2017-08-08T09:38">nfrast</ins><ins cite="mailto:Apri%20Listiyanto" datetime="20

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Ackermann, K. E., B. G. Gish, M. P. Honchar, and W. R. Sherman. "Evidence that inositol 1-phosphate in brain of lithium-treated rats results mainly from phosphatidylinositol metabolism." Biochemical Journal 242, no. 2 (1987): 517–24. http://dx.doi.org/10.1042/bj2420517.

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In cerebral cortex of rats treated with increasing doses of LiCl, the relative concentrations of Ins(1)P, Ins(4)P and Ins(5)P (when InsP is a myo-inositol phosphate) are approx. 10:1:0.2 at all doses. In rats treated with LiCl followed by increasing doses of pilocarpine a similar relationship occurs. myo-Inositol-1-phosphatase (InsP1ase) from bovine brain hydrolyses Ins(1)P, Ins(4)P and Ins(5)P at comparable rates, and these substrates have similar Km values. The hydrolysis of Ins(4)P is inhibited by Li+ to a greater degree than is hydrolysis of Ins(1)P and Ins(5)P. D-Ins(1,4,5)P3 and D-Ins(1,

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Kuo, Shu-Chen, Yung-Chih Wang, Mei-Chen Tan, et al. "In vitro activity of imipenem/relebactam, meropenem/vaborbactam, ceftazidime/avibactam, cefepime/zidebactam and other novel antibiotics against imipenem-non-susceptible Gram-negative bacilli from Taiwan." Journal of Antimicrobial Chemotherapy 76, no. 8 (2021): 2071–78. http://dx.doi.org/10.1093/jac/dkab141.

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Abstract Objectives To investigate the susceptibility of imipenem-non-susceptible Escherichia coli (INS-EC), Klebsiella pneumoniae (INS-KP), Acinetobacter baumannii (INS-AB) and Pseudomonas aeruginosa (INS-PA) to novel antibiotics. Methods MICs were determined using the broth microdilution method. Carbapenemase and ESBL phenotypic testing and PCR for genes encoding ESBLs, AmpCs and carbapenemases were performed. Results Zidebactam, avibactam and relebactam increased the respective susceptibility rates to cefepime, ceftazidime and imipenem of 17 INS-EC by 58.8%, 58.8% and 70.6%, of 163 INS-KP b

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Salamun, Tri. "PELAKSANAAN IZIN GANGGUAN DALAM USAHA KEDAI KOPI DI KOTA BANDA ACEH." Jurnal Rechts Vinding: Media Pembinaan Hukum Nasional 7, no. 3 (2018): 409. http://dx.doi.org/10.33331/rechtsvinding.v7i3.270.

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<p><ins cite="mailto:Windows%20User" datetime="2018-11-06T09:57">Keberadaan kedai kopi di Kota Banda Aceh </ins><ins cite="mailto:Windows%20User" datetime="2018-11-06T09:58">semakin marak seiring dengan pulihnya kondisi perekonomian masyarakat pasca bencana Tsunami yang melanda Aceh pada akhir Tahun 2004.</ins><ins cite="mailto:Windows%20User" datetime="2018-11-06T09:59"> Bisnis </ins><ins cite="mailto:Windows%20User" datetime="2018-11-06T09:56">café dan kedai kopi khususnya di Kota Banda Aceh</ins><ins cite="mailto:Windows%20User" datet

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Cullen, P. J., R. F. Irvine, and A. P. Dawson. "Synergistic control of Ca2+ mobilization in permeabilized mouse L1210 lymphoma cells by inositol 2,4,5-trisphosphate and inositol 1,3,4,5-tetrakisphosphate." Biochemical Journal 271, no. 2 (1990): 549–53. http://dx.doi.org/10.1042/bj2710549.

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L1210 lymphoma cells were permeabilized with digitonin, and the ability of Ins(2,4,5)P3 and Ins(1,3,4,5)P4 to mobilize intracellular Ca2+ was studied. At high doses of Ins(2,4,5)P3 Ca2+ was rapidly released from intracellular stores, and prior or subsequent addition of Ins(1,3,4,5)P4 had no discernible effect. However, the Ca2(+)-mobilizing action of low (threshold or just above) concentrations of Ins(2,4,5)P3 was markedly enhanced by Ins(1,3,4,5)P4, which alone caused no mobilization of Ca2+; this phenomenon was shown not to be due to protection of Ins(2,4,5)P3 by the Ins(1,3,4,5)P4 against h

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Al-Dhahir, W., A. Mubashir, and D. S. Andole. "Total ophthalmoplegia /INS;due to giant /INS;cell arteritis." Journal of the Neurological Sciences 333 (October 2013): e699. http://dx.doi.org/10.1016/j.jns.2013.07.2416.

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Narahara, Kouji. "Dir ins(9)(q34.3q22.1q31.3) or inv ins(9)(q34.3q22.3q21.2)?" Japanese journal of human genetics 32, no. 1 (1987): 49–50. http://dx.doi.org/10.1007/bf01876528.

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Benecke, R., D. Hauschke, and P. Roggenkämper. "Incobotulinum /INS;toxin /INS;A demonstrated therapeutic equivalence to onabotulinum /INS;toxin /INS;A in the treatment of blepharospasm and cervical dystonia." Journal of the Neurological Sciences 333 (October 2013): e120. http://dx.doi.org/10.1016/j.jns.2013.07.402.

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Greiner, Ralf, and Nils-Gunnar Carlsson. "myo-Inositol phosphate isomers generated by the action of a phytate-degrading enzyme from Klebsiella terrigena on phytate." Canadian Journal of Microbiology 52, no. 8 (2006): 759–68. http://dx.doi.org/10.1139/w06-028.

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For the first time a dual pathway for dephosphorylation of myo-inositol hexakisphosphate by a histidine acid phytase was established. The phytate-degrading enzyme of Klebsiella terrigena degrades myo-inositol hexakisphosphate by stepwise dephosphorylation, preferably via D-Ins(1,2,4,5,6)P5, D-Ins(1,2,5,6)P4, D-Ins(1,2,6)P3, D-Ins(1,2)P2 and alternatively via D-Ins(1,2,4,5,6)P5, Ins(2,4,5,6)P4, D-Ins(2,4,5)P3, D-Ins(2,4)P2 to finally Ins(2)P. It was estimated that more than 98% of phytate hydrolysis occurs via D-Ins(1,2,4,5,6)P5. Therefore, the phytate-degrading enzyme from K. terrigena has to

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Wilcox, R. A., R. A. Challiss, G. Baudin, A. Vasella, B. V. Potter, and S. R. Nahorski. "Stereoselectivity of Ins(1,3,4,5)P4 recognition sites: implications for the mechanism of the Ins(1,3,4,5)P4-induced Ca2+ mobilization." Biochemical Journal 294, no. 1 (1993): 191–94. http://dx.doi.org/10.1042/bj2940191.

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Ins(1,3,4,5)P4 was able to mobilize the entire Ins(1,4,5)P3-sensitive intracellular Ca2+ store in saponin-permeabilized SH-SY5Y human neuroblastoma cells in a concentration-dependent manner, yielding an EC50 value of 2.05 +/- 0.45 microM, compared with 0.14 +/- 0.03 microM for Ins(1,4,5)P3. However, L-Ins(1,3,4,5)P4 [= D-Ins(1,3,5,6)P4] failed to cause mobilization of intracellular Ca2+ at concentrations up to 100 microM. Binding studies using pig cerebellar membranes as a source of both Ins(1,4,5)P3/Ins(1,3,4,5)P4-specific binding sites have revealed a marked contrast in their stereospecifici

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Barker, C. J., N. S. Wong, S. M. Maccallum, P. A. Hunt, R. H. Michell, and C. J. Kirk. "The interrelationships of the inositol phosphates formed in vasopressin-stimulated WRK-1 rat mammary tumour cells." Biochemical Journal 286, no. 2 (1992): 469–74. http://dx.doi.org/10.1042/bj2860469.

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1. Temporal changes in the levels of many inositol phosphates, whose structural characterization is presented in the preceding paper [Wong, Barker, Morris, Craxton, Kirk & Michell (1991) Biochem. J. 286, 459-468], have been monitored in vasopressin-stimulated WRK-1 cells. 2. Upon stimulation, Ins(1,4,5)P3 accumulated within 1 s, consistent with its role as a rapidly acting second messenger produced by receptor activation of phosphoinositidase C. Ins(1,4)P2 and Ins(1,3,4,5)P4, both of which are immediate products of Ins(1,4,5)P3 metabolism, also accumulated quickly. Ins4P, Ins(1,3,4)P3, Ins

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Afolabi, Folashade, and Oluwafemi Abayomi Olajuyigbe. "Building Resilience in Education for Academic Continuity During Disruption." International Journal of Pedagogy and Teacher Education 7, no. 1 (2022): 13. http://dx.doi.org/10.20961/ijpte.v7i1.62396.

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<p class="p1"><ins cite="mailto:proofreader" datetime="2023-04-23T16:50">T</ins>he traditional method of learning prevailed in the educational system <ins cite="mailto:proofreader" datetime="2023-04-26T14:15">up </ins>until the <ins cite="mailto:proofreader" datetime="2023-04-23T16:46">out</ins>break<del cite="mailto:proofreader" datetime="2023-04-23T16:46"> out</del> of the COVID-19 pandemic<ins cite="mailto:proofreader" datetime="2023-04-23T16:50">,</ins> which brought a turning point by digitali<ins cite="mailto:proofreade

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Wong, N. S., C. J. Barker, A. J. Morris, A. Craxton, C. J. Kirk, and R. H. Michell. "The inositol phosphates in WRK1 rat mammary tumour cells." Biochemical Journal 286, no. 2 (1992): 459–68. http://dx.doi.org/10.1042/bj2860459.

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1. A detailed structural survey has been made of the inositol phosphates of unstimulated and vasopressin-stimulated WRK-1 rat mammary tumour cells. Inositol phosphate peaks were separated by h.p.l.c., and structural assignments were made for more than 20 compounds by combinations of: (a) co-chromatography with labelled standards; (b) site-specific enzymic dephosphorylation; (c) complete and partial periodate oxidation, followed by h.p.l.c. of polyols and their stereospecific oxidation by dehydrogenases; and (d) ammoniacal hydrolysis. 2. The ‘inositol monophosphates’ fraction from unstimulated

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Suharyo, Suharyo. "PROBLEMATIKA PENEGAKAN HUKUM PENATAAN RUANG DALAM PELAKSANAAN OTONOMI DAERAH." Jurnal Rechts Vinding: Media Pembinaan Hukum Nasional 6, no. 2 (2017): 171. http://dx.doi.org/10.33331/rechtsvinding.v6i2.185.

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<p><ins cite="mailto:TDA%20P" datetime="2017-08-22T08:32">Dalam dinamika kehidupan masyarakat yang semakin berkembang pesat dan maju dengan perekonomian yang membaik, tata ruang menjadi hal yang sangat strategis untuk menccapai ketertiban, keserasian, kesejahteraan</ins>, <ins cite="mailto:TDA%20P" datetime="2017-08-22T08:32">dan ketenteraman masyarakat. Sebagai kebijakan negara telah dikeluarkan Undang-Undang Nomor 26 </ins>T<ins cite="mailto:TDA%20P" datetime="2017-08-22T08:32">ahun 2007 tentang Penataan Ruang. Dalam era otonomi daerah</ins> juga tel

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HIRATA, Masato, Hiroshi TAKEUCHI, M. Andrew RILEY, J. Stephen MILLS, Yutaka WATANABE, and V. L. Barry POTTER. "Inositol 1,4,5-trisphosphate receptor subtypes differentially recognize regioisomers of D-myo-inositol 1,4,5-trisphosphate." Biochemical Journal 328, no. 1 (1997): 93–98. http://dx.doi.org/10.1042/bj3280093.

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The Ins(1,4,5)P3 regioisomers, Ins(1,4,6)P3 and Ins(1,3,6)P3, which can mimic the 1,4,5-arrangement on the inositol ring of Ins(1,4,5)P3, were examined for Ca2+ release by using four types of saponin-permeabilized cell possessing various abundances of receptor subtypes, with special reference to the relation of potency to receptor subtype. Ins(1,4,6)P3 and Ins(1,3,6)P3 were weak agonists in rat basophilic leukaemic cells (RBL cells), which possess predominantly subtype II receptors, with respective potencies of 1/200 and less than 1/500 that of Ins(1,4,5)P3 [the EC50 values were 0.2, 45 and mo

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38

Shieh, W. R., and C. S. Chen. "Preparation and characterization of a d-myo-inositol 1,4,5-trisphosphate-specific antibody." Biochemical Journal 311, no. 3 (1995): 1009–14. http://dx.doi.org/10.1042/bj3111009.

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Abstract (sommario):

Antibodies against Ins(1,4,5)P3 were raised by immunizing rabbits with two types of InsP3-BSA conjugates which were synthesized by covalently coupling Ins(1,4,5)P3 to the carrier protein via alkyl linkages. The anti-Ins(1,4,5)P3 antibody was detected by a novel ELISA using Ins(1,4,5)P3-immobilized microtitre plates. Both antiserum preparations showed specific binding with Ins(1,4,5)P3, with titres of 1:4000. Most inositol phosphates, including Ins1P, Ins(4,5)P2, Ins(1,3,4)P3, Ins(1,5,6)P3, Ins(1,2,5,6)P1, Ins(3,4,5,6)P4, Ins(1,3,4,5,6)P5, InsP6, and PtdIns(4,5)P2, did not exhibit significant m

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39

ONGUSAHA, Pat P., Philip J. HUGHES, John DAVEY, and Robert H. MICHELL. "Inositol hexakisphosphate in Schizosaccharomyces pombe: synthesis from Ins(1,4,5)P3 and osmotic regulation." Biochemical Journal 335, no. 3 (1998): 671–79. http://dx.doi.org/10.1042/bj3350671.

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Abstract (sommario):

Schizosaccharomyces pombe extracts synthesize InsP6 (myo-inositol hexaphosphate) from Ins(1,4,5)P3 plus ATP. An S. pombe soluble fraction converts Ins(1,4,5)P3 into Ins(1,4,5,6)P4 and Ins(1,3,4,5)P4, in a constant ratio of ≈ 5:1, and thence to Ins(1,3,4,5,6)P5 and InsP6. We have purified a soluble Mg2+-dependent kinase of molecular mass ≈ 41 kDa that makes Ins(1,4,5,6)P4 and Ins(1,3,4,5)P4 in the same ratio and also converts Ins(1,4,5,6)P4 or Ins(1,3,4,5)P4 into Ins(1,3,4,5,6)P5 and InsP6. Of InsP3 isomers other than Ins(1,4,5)P3, only the non-biological molecule Ins(1,4,6)P3 potently ‘compete

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40

Guse, A. H., I. Berg та G. Gercken. "Metabolism of inositol phosphates in α1-adrenoceptor-stimulated and homogenized cardiac myocytes of adult rats". Biochemical Journal 261, № 1 (1989): 89–92. http://dx.doi.org/10.1042/bj2610089.

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Abstract (sommario):

Previous studies demonstrated the accumulation of inositol mono- and poly-phosphates in carbamoylcholine-stimulated cultured cardiac ventricular myocytes of adult rats [Berg, Guse & Gercken (1989) Biochim. Biophys. Acta 1010, 100-107]. Stimulation with noradrenaline (50 microM) in the presence of propranolol (10 microM) led to a time-dependent pattern of inositol mono- and poly-phosphates in cultured cardiac-ventricular myocytes. Ins(1,4,5)P3 and Ins(1,3,4,5)P4 increased in a rapid initial phase. The degradation products of Ins(1,4,5)P3, namely Ins(1,4)P2 and Ins(4)P, accumulated between 1

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41

Hirata, M., Y. Kimura, T. Ishimatsu, et al. "Synthetic inositol 1,3,4,5-tetrakisphosphate analogues." Biochemical Journal 276, no. 2 (1991): 333–36. http://dx.doi.org/10.1042/bj2760333.

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Abstract (sommario):

Inositol 1,3,4,5-tetrakisphosphate [Ins(1,3,4,5)P4] analogues were synthesized and their effects on [3H]Ins(1,3,4,5)P4 5-phosphatase, [3H]Ins(1,3,4,5)P4 3-phosphatase and [3H]inositol 1,4,5-trisphosphate [3H]Ins(1,4,5)P3] 5-phosphatase activities were examined. The Ins(1,3,4,5)P4 analogue with the aminobenzoyl group at the 2-position of Ins(1,3,4,5)P4 inhibited the hydrolysis of 5-phosphate of [3H]Ins(1,3,4,5)P4 catalysed by erythrocyte ghosts, with a lower Ki value than seen with Ins(1,3,4,5)P4, whereas the analogue with the aminocyclohexanecarbonyl group at the same position had a higher Ki

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42

Naito, Yusuke, Kensuke Tanaka, Kento Yoshioka, Koichiro Tatsumi, Sadao Kimura, and Yoshitoshi Kasuya. "Effects of selective ETB receptor antagonist on the /INS;brain of/INS; /INS;sepsis mouse model." Life Sciences 93, no. 25-26 (2013): e76. http://dx.doi.org/10.1016/j.lfs.2014.01.055.

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43

Wang, Shuangqing, Saige Meng, Xinlei Zhou, Zhonggao Gao, and Ming Guan Piao. "pH-Responsive and Mucoadhesive Nanoparticles for Enhanced Oral Insulin Delivery: The Effect of Hyaluronic Acid with Different Molecular Weights." Pharmaceutics 15, no. 3 (2023): 820. http://dx.doi.org/10.3390/pharmaceutics15030820.

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Abstract (sommario):

Drug degradation at low pH and rapid clearance from intestinal absorption sites are the main factors limiting the development of oral macromolecular delivery systems. Based on the pH responsiveness and mucosal adhesion of hyaluronic acid (HA) and poly[2-(dimethylamino)ethyl methacrylate] (PDM), we prepared three HA–PDM nano-delivery systems loaded with insulin (INS) using three different molecular weights (MW) of HA (L, M, H), respectively. The three types of nanoparticles (L/H/M-HA–PDM–INS) had uniform particle sizes and negatively charged surfaces. The optimal drug loadings of the L-HA–PDM–I

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44

Van Lookeren Campagne, M. M., C. Erneux, R. Van Eijk, and P. J. M. Van Haastert. "Two dephosphorylation pathways of inositol 1,4,5-trisphosphate in homogenates of the cellular slime mould Dictyostelium discoideum." Biochemical Journal 254, no. 2 (1988): 343–50. http://dx.doi.org/10.1042/bj2540343.

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Abstract (sommario):

Dictyostelium discoideum homogenates contain phosphatase activity which rapidly dephosphorylates Ins(1,4,5)P3 (D-myo-inositol 1,4,5-trisphosphate) to Ins (myo-inositol). When assayed in Mg2+, Ins(1,4,5)P3 is dephosphorylated by the soluble Dictyostelium cell fraction to 20% Ins(1,4)P2 (D-myo-inositol 1,4-bisphosphate) and 80% Ins(4,5)P2 (D-myo-inositol 4,5-bisphosphate). In the particulate fraction Ins(1,4,5)P3 5-phosphatase is relatively more active than the Ins(1,4,5)P3 1-phosphatase. CaCl2 can replace MgCl2 only for the Ins(1,4,5)P3 5-phosphatase activity. Ins(1,4)P2 and Ins(4,5)P2 are both

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45

Beinotti, F., K. Alonso, E. Azevedo, and A. Cliquet Junior. "Neuromuscular electrical stimulation applied to upper limb flexor /INS;muscles of tetraplegics: Interlimb reflex /INS;response assessed by electromyography /INS;— /INS;Pilot study." Journal of the Neurological Sciences 333 (October 2013): e548-e549. http://dx.doi.org/10.1016/j.jns.2013.07.1927.

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46

Taylor, C. W., and B. V. L. Potter. "The size of inositol 1,4,5-trisphosphate-sensitive Ca2+ stores depends on inositol 1,4,5-trisphosphate concentration." Biochemical Journal 266, no. 1 (1990): 189–94. http://dx.doi.org/10.1042/bj2660189.

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Abstract (sommario):

An explanation of the complex effects of hormones on intracellular Ca2+ requires that the intracellular actions of Ins(1,4,5)P3 and the relationships between intracellular Ca2+ stores are fully understood. We have examined the kinetics of 45Ca2+ efflux from pre-loaded intracellular stores after stimulation with Ins(1,4,5)P3 or the stable phosphorothioate analogue, Ins(1,4,5)P3[S]3, by simultaneous addition of one of them with glucose/hexokinase to rapidly deplete the medium of ATP. Under these conditions, a maximal concentration of either Ins(1,4,5)P3 or Ins(1,4,5)P3[S]3 evoked rapid efflux of

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47

Gawler, D. J., B. V. L. Potter, R. Gigg, and S. R. Nahorski. "Interactions between inositol tris- and tetrakis-phosphates. Effects on intracellular Ca2+ mobilization in SH-SY5Y cells." Biochemical Journal 276, no. 1 (1991): 163–67. http://dx.doi.org/10.1042/bj2760163.

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Abstract (sommario):

The potential Ca2(+)-releasing activity of the inositol tetrakisphosphates Ins(1,3,4,6)P4 and DL-Ins(1,4,5,6)P4 and the inositol pentakisphosphate Ins(1,3,4,5,6)P5 and their effect on Ins(1,4,5)P3- and DL-Ins (1,3,4,5)P4-mediated Ca2+ release were examined in permeabilized SH-SY5Y human neuroblastoma cells. Neither DL-Ins(1,4,5,6)P4 nor Ins(1,3,4,5,6)P5 exhibit Ca2(+)-releasing activity at concentrations up to 10 microM, but Ins(1,3,4,6)P4 releases Ca2+ dose-dependently, with an EC50 value (conen, giving half-maximal effect) of 5.92 +/- 0.47 microM. Maximal response by this tetrakisphosphate (

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48

Taylor, C. W., M. J. Berridge, A. M. Cooke, and B. V. L. Potter. "Inositol 1,4,5-trisphosphorothioate, a stable analogue of inositol trisphosphate which mobilizes intracellular calcium." Biochemical Journal 259, no. 3 (1989): 645–50. http://dx.doi.org/10.1042/bj2590645.

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Abstract (sommario):

D-Ins(1,4,5)P3 is now recognized as an intracellular messenger that mediates the actions of many cell-surface receptors on intracellular Ca2+ pools, but its complex and rapid metabolism in intact cells has confused interpretation of its possible roles in oscillatory changes in intracellular [Ca2+] and in controlling Ca2+ entry at the plasma membrane. We now report the actions and metabolic stability of a synthetic analogue of Ins(1,4,5)P3, DL-inositol 1,4,5-trisphosphorothioate [DL-Ins(1,4,5)P3[S]3]. In permeabilized hepatocytes, DL-Ins(1,4,5)P3[S]3 and synthetic DL-Ins(1,4,5)P3 stimulated Ca2

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49

Gawler, D. J., B. V. Potter, and S. R. Nahorski. "Inositol 1,3,4,5-tetrakisphosphate-induced release of intracellular Ca2+ in SH-SY5Y neuroblastoma cells." Biochemical Journal 272, no. 2 (1990): 519–24. http://dx.doi.org/10.1042/bj2720519.

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Abstract (sommario):

Inositol-polyphosphate-induced Ca2+ mobilization was investigated in saponin-permeabilized SH-SY5Y human neuroblastoma cells. Ins(1,4,5)P3 induced a dose-related release from intracellular Ca2+ stores with an EC50 (concn. giving half-maximal effect) of 0.1 microM and a maximal release of 70%. Ins(1,3,4)P3, DL-Ins(1,4,5,6)P4 and Ins(1,3,4,5,6)P5 did not evoke Ca2+ mobilization in these cells when used at concentrations up to 10 microM. However, Ins(1,3,4,5)P4 was found to release Ca2+ in a dose-related manner, but the response was dependent on the source of Ins(1,3,4,5)P4 used. When commerciall

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50

Erneux, C., A. Delvaux, C. Moreau, and J. E. Dumont. "The dephosphorylation pathway of d-myo-inositol 1,3,4,5-tetrakisphosphate in rat brain." Biochemical Journal 247, no. 3 (1987): 635–39. http://dx.doi.org/10.1042/bj2470635.

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Abstract (sommario):

Dephosphorylation of inositol 1,3,4,5-tetrakisphosphate [Ins(1,3,4,5)P4] was measured in both the soluble and the particulate fractions of rat brain homogenates. Analysis of the hydrolysis of [4,5-32P]Ins(1,3,4,5)P4 showed that for both fractions the 5-phosphate of Ins(1,3,4,5)P4 was removed and inositol 1,3,4-trisphosphate [Ins(1,3,4)P3] was specifically produced. In the soluble fraction, Ins(1,3,4)P3 was further hydrolysed at the 1-phosphate position to inositol 3,4-bisphosphate[Ins(3,4)P2]. DEAE-cellulose chromatography of the soluble fraction separated the phosphatase activities into three

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