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Articoli di riviste sul tema "Integrative and mobilizable element"

Autore: Grafiati
Pubblicato: 14 marzo 2023
Ultima modifica: 31 luglio 2025

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1

Doublet, Benoît, David Boyd, Michael R. Mulvey, and Axel Cloeckaert. "TheSalmonellagenomic island 1 is an integrative mobilizable element." Molecular Microbiology 55, no. 6 (2005): 1911–24. http://dx.doi.org/10.1111/j.1365-2958.2005.04520.x.

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2

López de Egea, Guillem, Aida González-Díaz, Gérard Guédon, et al. "A New Integrative and Mobilizable Element Is a Major Contributor to Tetracycline Resistance in Streptococcus dysgalactiae subsp. equisimilis." Antibiotics 12, no. 3 (2023): 579. http://dx.doi.org/10.3390/antibiotics12030579.

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Tetracycline resistance in streptococci is mainly due to ribosomal protection mediated by the tet(M) gene that is usually located in the integrative and conjugative elements (ICEs) of the Tn916-family. In this study, we analyzed the genes involved in tetracycline resistance and the associated mobile genetic elements (MGEs) in Streptococcus dysgalactiae subsp. equisimilis (SDSE) causing invasive disease. SDSE resistant to tetracycline collected from 2012 to 2019 in a single hospital and from 2018 in three other hospitals were analyzed by whole genome sequencing. Out of a total of 84 SDSE isolat
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3

Lorenzo-Diaz, Fabian, Cris Fernández-Lopez, Pierre-Emmanuel Douarre, et al. "Streptococcal group B integrative and mobilizable element IMESag- rpsI encodes a functional relaxase involved in its transfer." Open Biology 6, no. 10 (2016): 160084. http://dx.doi.org/10.1098/rsob.160084.

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Streptococcus agalactiae or Group B Streptococcus (GBS) are opportunistic bacteria that can cause lethal sepsis in children and immuno-compromised patients. Their genome is a reservoir of mobile genetic elements that can be horizontally transferred. Among them, integrative and conjugative elements (ICEs) and the smaller integrative and mobilizable elements (IMEs) primarily reside in the bacterial chromosome, yet have the ability to be transferred between cells by conjugation. ICEs and IMEs are therefore a source of genetic variability that participates in the spread of antibiotic resistance. A
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4

Lao, Julie, Gérard Guédon, Thomas Lacroix, et al. "Abundance, Diversity and Role of ICEs and IMEs in the Adaptation of Streptococcus salivarius to the Environment." Genes 11, no. 9 (2020): 999. http://dx.doi.org/10.3390/genes11090999.

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Streptococcus salivarius is a significant contributor to the human oral, pharyngeal and gut microbiomes that contribute to the maintenance of health. The high genomic diversity observed in this species is mainly caused by horizontal gene transfer. This work aimed to evaluate the contribution of integrative and conjugative elements (ICEs) and integrative and mobilizable elements (IMEs) in S. salivarius genome diversity. For this purpose, we performed an in-depth analysis of 75 genomes of S. salivarius and searched for signature genes of conjugative and mobilizable elements. This analysis led to
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5

Durand, Romain, Florence Deschênes, and Vincent Burrus. "Genomic islands targeting dusA in Vibrio species are distantly related to Salmonella Genomic Island 1 and mobilizable by IncC conjugative plasmids." PLOS Genetics 17, no. 8 (2021): e1009669. http://dx.doi.org/10.1371/journal.pgen.1009669.

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Salmonella Genomic Island 1 (SGI1) and its variants are significant contributors to the spread of antibiotic resistance among Gammaproteobacteria. All known SGI1 variants integrate at the 3’ end of trmE, a gene coding for a tRNA modification enzyme. SGI1 variants are mobilized specifically by conjugative plasmids of the incompatibility groups A and C (IncA and IncC). Using a comparative genomics approach based on genes conserved among members of the SGI1 group, we identified diverse integrative elements distantly related to SGI1 in several species of Vibrio, Aeromonas, Salmonella, Pokkaliibact
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6

Woudstra, Cedric, and Sophie A. Granier. "A Glimpse at the Anti-Phage Defenses Landscape in the Foodborne Pathogen Salmonella enterica subsp. enterica Serovar Typhimurium." Viruses 15, no. 2 (2023): 333. http://dx.doi.org/10.3390/v15020333.

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Bacteriophages, which specifically infect and kill bacteria, are currently used as additives to control pathogens such as Salmonella in human food (PhageGuard S®) or animal feed (SalmoFREE®, Bafasal®). Indeed, salmonellosis is among the most important zoonotic foodborne illnesses. The presence of anti-phage defenses protecting bacteria against phage infection could impair phage applications aiming at reducing the burden of foodborne pathogens such as Salmonella enterica subsp. enterica serovar Typhimurium (S. Typhimurium) to the food industry. In this study, the landscape of S. Typhimurium ant
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7

He, Jiaqi, Xiuhui Lu, Chenchen Yuan, et al. "Genetic Characteristics of Novel IncpSE5381-aadB Plasmids, Integrative and Mobilizable Elements, and Integrative and Conjugative Elements in Pseudomonas aeruginosa." Infection and Drug Resistance Volume 17 (May 2024): 2053–68. http://dx.doi.org/10.2147/idr.s462670.

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8

Libante, Virginie, Nazim Sarica, Abbas Mohamad Ali, et al. "Mobilization of IMEs Integrated in the oriT of ICEs Involves Their Own Relaxase Belonging to the Rep-Trans Family of Proteins." Genes 11, no. 9 (2020): 1004. http://dx.doi.org/10.3390/genes11091004.

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Integrative mobilizable elements (IMEs) are widespread but very poorly studied integrated elements that can excise and hijack the transfer apparatus of co-resident conjugative elements to promote their own spreading. Sixty-four putative IMEs, harboring closely related mobilization and recombination modules, were found in 14 Streptococcus species and in Staphylococcus aureus. Fifty-three are integrated into the origin of transfer (oriT) of a host integrative conjugative element (ICE), encoding a MobT relaxase and belonging to three distant families: ICESt3, Tn916, and ICE6013. The others are in
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9

Wang, Jun, Nadja B. Shoemaker, Gui-Rong Wang, and Abigail A. Salyers. "Characterization of a Bacteroides Mobilizable Transposon, NBU2, Which Carries a Functional Lincomycin Resistance Gene." Journal of Bacteriology 182, no. 12 (2000): 3559–71. http://dx.doi.org/10.1128/jb.182.12.3559-3571.2000.

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ABSTRACT The mobilizable Bacteroides element NBU2 (11 kbp) was found originally in two Bacteroides clinical isolates,Bacteroides fragilis ERL and B. thetaiotaomicron DOT. At first, NBU2 appeared to be very similar to another mobilizable Bacteroides element, NBU1, in a 2.5-kbp internal region, but further examination of the full DNA sequence of NBU2 now reveals that the region of near identity between NBU1 and NBU2 is limited to this small region and that, outside this region, there is little sequence similarity between the two elements. The integrase gene of NBU2, intN2, was located at one end
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10

Shoemaker, N. B., G. R. Wang, A. M. Stevens, and A. A. Salyers. "Excision, transfer, and integration of NBU1, a mobilizable site-selective insertion element." Journal of Bacteriology 175, no. 20 (1993): 6578–87. http://dx.doi.org/10.1128/jb.175.20.6578-6587.1993.

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11

Veschetti, Laura, Angela Sandri, Helle Krogh Johansen, Maria M. Lleò, and Giovanni Malerba. "Hypermutation as an Evolutionary Mechanism for Achromobacter xylosoxidans in Cystic Fibrosis Lung Infection." Pathogens 9, no. 2 (2020): 72. http://dx.doi.org/10.3390/pathogens9020072.

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Achromobacter xylosoxidans can cause chronic infections in the lungs of patients with cystic fibrosis (CF) by adapting to the specific environment. The study of longitudinal isolates allows to investigate its within-host evolution to unravel the adaptive mechanisms contributing to successful colonization. In this study, four clinical isolates longitudinally collected from two chronically infected patients underwent whole genome sequencing, de novo assembly and sequence analysis. Phenotypic assays were also performed. The isolates coming from one of the patients (patient A) presented a greater
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12

Berbel, Dàmaris, Jordi Càmara, Aida González-Díaz, et al. "Deciphering mobile genetic elements disseminating macrolide resistance in Streptococcus pyogenes over a 21 year period in Barcelona, Spain." Journal of Antimicrobial Chemotherapy 76, no. 8 (2021): 1991–2003. http://dx.doi.org/10.1093/jac/dkab130.

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Abstract Objectives To phenotypically and genetically characterize the antibiotic resistance determinants and associated mobile genetic elements (MGEs) among macrolide-resistant (MR) Streptococcus pyogenes [Group A streptococci (GAS)] clinical isolates collected in Barcelona, Spain. Methods Antibiotic susceptibility testing was performed by microdilution. Isolates were emm and MLST typed and 55 were whole-genome sequenced to determine the nature of the macrolide resistance (MR) determinants and their larger MGE and chromosomal context. Results Between 1998 and 2018, 142 of 1028 GAS (13.8%) wer
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13

Libante, Virginie, Yves Nombre, Charles Coluzzi, et al. "Chromosomal Conjugative and Mobilizable Elements in Streptococcus suis: Major Actors in the Spreading of Antimicrobial Resistance and Bacteriocin Synthesis Genes." Pathogens 9, no. 1 (2019): 22. http://dx.doi.org/10.3390/pathogens9010022.

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Streptococcus suis is a zoonotic pathogen suspected to be a reservoir of antimicrobial resistance (AMR) genes. The genomes of 214 strains of 27 serotypes were screened for AMR genes and chromosomal Mobile Genetic Elements (MGEs), in particular Integrative Conjugative Elements (ICEs) and Integrative Mobilizable Elements (IMEs). The functionality of two ICEs that host IMEs carrying AMR genes was investigated by excision tests and conjugation experiments. In silico search revealed 416 ICE-related and 457 IME-related elements. These MGEs exhibit an impressive diversity and plasticity with tandem a
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14

Pavlovic, Guillaume, Vincent Burrus, Brigitte Gintz, Bernard Decaris, and Gérard Guédon. "Evolution of genomic islands by deletion and tandem accretion by site-specific recombination: ICESt1-related elements from Streptococcus thermophilus." Microbiology 150, no. 4 (2004): 759–74. http://dx.doi.org/10.1099/mic.0.26883-0.

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The 34 734-bp integrative and potentially conjugative element (putative ICE) ICESt1 has been previously found to be site-specifically integrated in the 3′ end of the fda locus of Streptococcus thermophilus CNRZ368. Four types of genomic islands related to ICESt1 are integrated in the same position in seven other strains of S. thermophilus. One of these elements, ICESt3, harbours conjugation and recombination modules closely related to those of ICESt1 and excises by site-specific recombination. Two other types of elements, CIME19258 and CIME302, are flanked by site-specific attachment sites clo
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15

Belhocine, Kamila, Karen K. Yam, and Benoit Cousineau. "Conjugative Transfer of the Lactococcus lactis Chromosomal Sex Factor Promotes Dissemination of the Ll.LtrB Group II Intron." Journal of Bacteriology 187, no. 3 (2005): 930–39. http://dx.doi.org/10.1128/jb.187.3.930-939.2005.

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ABSTRACT The Ll.LtrB group II intron from the low-G+C gram-positive bacterium Lactococcus lactis was the first bacterial group II intron shown to splice and mobilize in vivo. This retroelement interrupts the relaxase gene (ltrB) of three L. lactis conjugative elements: plasmids pRS01 and pAH90 and the chromosomal sex factor. Conjugative transfer of a plasmid harboring a segment of the pRS01 conjugative plasmid including the Ll.LtrB intron allows dissemination of Ll.LtrB among L. lactis strains and lateral transfer of this retroelement from L. lactis to Enterococcus faecalis. Here we report the
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16

Alvarez Narvaez, Sonsiray, and Susan Sanchez. "Exploring the Accessory Genome of Multidrug-Resistant Rhodococcus equi Clone 2287." Antibiotics 12, no. 11 (2023): 1631. http://dx.doi.org/10.3390/antibiotics12111631.

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Decades of antimicrobial overuse to treat respiratory disease in foals have promoted the emergence and spread of zoonotic multidrug-resistant (MDR) Rhodococcus equi worldwide. Three main R. equi MDR clonal populations—2287, G2106, and G2017—have been identified so far. However, only clones 2287 and G2016 have been isolated from sick animals, with clone 2287 being the main MDR R. equi recovered. The genetic mechanisms that make this MDR clone superior to the others at infecting foals are still unknown. Here, we performed a deep genetic characterization of the accessory genomes of 207 R. equi is
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17

LeGault, Kristen N., Stephanie G. Hays, Angus Angermeyer, et al. "Temporal shifts in antibiotic resistance elements govern phage-pathogen conflicts." Science 373, no. 6554 (2021): eabg2166. http://dx.doi.org/10.1126/science.abg2166.

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Bacteriophage predation selects for diverse antiphage systems that frequently cluster on mobilizable defense islands in bacterial genomes. However, molecular insight into the reciprocal dynamics of phage-bacterial adaptations in nature is lacking, particularly in clinical contexts where there is need to inform phage therapy efforts and to understand how phages drive pathogen evolution. Using time-shift experiments, we uncovered fluctuations in Vibrio cholerae’s resistance to phages in clinical samples. We mapped phage resistance determinants to SXT integrative and conjugative elements (ICEs),
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18

Tarrah, Armin, Shadi Pakroo, Viviana Corich, and Alessio Giacomini. "Identification and Transferability of Tetracycline Resistance in Streptococcus thermophilus during Milk Fermentation, Storage, and Gastrointestinal Transit." Fermentation 7, no. 2 (2021): 65. http://dx.doi.org/10.3390/fermentation7020065.

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The existence of antibiotic-resistant bacteria in food products, particularly those carrying acquired resistance genes, has increased concerns about the transmission of these genes from beneficial microbes to human pathogens. In this study, we evaluated the antibiotic resistance-susceptibility patterns of 16 antibiotics in eight S. thermophilus strains, whose genome sequence is available, using phenotypic and genomic approaches. The minimal inhibitory concentration values collected revealed intermediate resistance to aminoglycosides, whereas susceptibility was detected for different classes of
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19

Schultz, Eliette, Marisa Haenni, Laurent Mereghetti, et al. "Survey of multidrug resistance integrative mobilizable elements SGI1 and PGI1 inProteus mirabilisin humans and dogs in France, 2010–13." Journal of Antimicrobial Chemotherapy 70, no. 9 (2015): 2543–46. http://dx.doi.org/10.1093/jac/dkv154.

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López, María, Beatriz Rojo-Bezares, Gabriela Chichón, and Yolanda Sáenz. "Resistance to Fluoroquinolones in Pseudomonas aeruginosa from Human, Animal, Food and Environmental Origin: The Role of CrpP and Mobilizable ICEs." Antibiotics 11, no. 9 (2022): 1271. http://dx.doi.org/10.3390/antibiotics11091271.

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Fluoroquinolone resistance and the associated genetic mechanisms were assessed by antimicrobial susceptibility and whole genome sequencing in 56 Pseudomonas aeruginosa strains from human, animal, food and environmental origins. P. aeruginosa PAO1, PA7 and PA14 reference strains were also included in the study. Twenty-two strains (37%) were resistant to, at least, one fluoroquinolone agent. Correlation between the number of changes in GyrA and ParC proteins and the level of fluoroquinolone resistance was observed. Mutations or absence of genes, such as mexZ, mvaT and nalD encoding efflux pumps
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Zhang, Yucheng, and Rosemary Loria. "Emergence of Novel Pathogenic Streptomyces Species by Site-Specific Accretion and cis-Mobilization of Pathogenicity Islands." Molecular Plant-Microbe Interactions® 30, no. 1 (2017): 72–82. http://dx.doi.org/10.1094/mpmi-09-16-0190-r.

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The main pathogenicity factor of Streptomyces species associated with the potato common scab disease is a nitrated diketopiperazine called thaxtomin A (ThxA). In Streptomyces scabiei (syn. S. scabies), which is thought to be the most ancient pathogenic Streptomyces species, the ThxA biosynthetic cluster is located within a mobile genomic island called the toxicogenic region (TR). Three attachment (att) sites further separate TR into two subregions (TR1 and TR2). TR1 contains the ThxA biosynthetic cluster and is conserved among several pathogenic Streptomyces species. However, TR2, an integrati
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Doublet, Benoît, Karine Praud, Sophie Bertrand, Jean-Marc Collard, François-Xavier Weill, and Axel Cloeckaert. "Novel Insertion Sequence- and Transposon-Mediated Genetic Rearrangements in Genomic Island SGI1 of Salmonella enterica Serovar Kentucky." Antimicrobial Agents and Chemotherapy 52, no. 10 (2008): 3745–54. http://dx.doi.org/10.1128/aac.00525-08.

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ABSTRACT Salmonella genomic island 1 (SGI1) is an integrative mobilizable element that harbors a multidrug resistance (MDR) gene cluster. Since its identification in epidemic Salmonella enterica serovar Typhimurium DT104 strains, variant SGI1 MDR gene clusters conferring different MDR phenotypes have been identified in several S. enterica serovars and classified as SGI1-A to -O. A study was undertaken to characterize SGI1 from serovar Kentucky strains isolated from travelers returning from Africa. Several strains tested were found to contain the partially characterized variant SGI1-K, recently
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23

Michaelis, Claudia, and Elisabeth Grohmann. "Horizontal Gene Transfer of Antibiotic Resistance Genes in Biofilms." Antibiotics 12, no. 2 (2023): 328. http://dx.doi.org/10.3390/antibiotics12020328.

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Most bacteria attach to biotic or abiotic surfaces and are embedded in a complex matrix which is known as biofilm. Biofilm formation is especially worrisome in clinical settings as it hinders the treatment of infections with antibiotics due to the facilitated acquisition of antibiotic resistance genes (ARGs). Environmental settings are now considered as pivotal for driving biofilm formation, biofilm-mediated antibiotic resistance development and dissemination. Several studies have demonstrated that environmental biofilms can be hotspots for the dissemination of ARGs. These genes can be encoded
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Lyras, Dena, Vicki Adams, Isabelle Lucet, and Julian I. Rood. "The large resolvase TnpX is the only transposon-encoded protein required for transposition of the Tn4451/3 family of integrative mobilizable elements." Molecular Microbiology 51, no. 6 (2004): 1787–800. http://dx.doi.org/10.1111/j.1365-2958.2003.03950.x.

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Xia, Yingjun, Zhaoyang Wang, Yanli Hu, et al. "Isolation, Identification, Genomic Diversity, and Antimicrobial Resistance Analysis of Streptococcus suis in Hubei Province of China from 2021 to 2023." Microorganisms 12, no. 5 (2024): 917. http://dx.doi.org/10.3390/microorganisms12050917.

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Streptococcus suis (S. suis) is a zoonotic pathogen capable of causing severe diseases in humans and pigs, including meningitis, sepsis, polyserositis, arthritis, and endocarditis. This study aimed to investigate the biological characteristics of 19 strains of S. suis isolated from diseased pigs in Hubei Province between 2021 and 2023. Through bioinformatics analysis, we investigated the serotype, MLST, pan-genome characteristics, SNP, AMR, and ICE of the 19 S. suis isolates. Among the 19 S. suis strains, ten serotypes were identified, and serotype 9 was the most prevalent (21.05%). Ten new al
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Puymège, Aurore, Stéphane Bertin, Gérard Guédon, and Sophie Payot. "Analysis of Streptococcus agalactiae pan-genome for prevalence, diversity and functionality of integrative and conjugative or mobilizable elements integrated in the tRNALys CTT gene." Molecular Genetics and Genomics 290, no. 5 (2015): 1727–40. http://dx.doi.org/10.1007/s00438-015-1031-9.

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Wang, Hongmei, Adam P. Roberts, Dena Lyras, Julian I. Rood, Mark Wilks, and Peter Mullany. "Characterization of the Ends and Target Sites of the Novel Conjugative Transposon Tn5397 from Clostridium difficile: Excision and Circularization Is Mediated by the Large Resolvase, TndX." Journal of Bacteriology 182, no. 13 (2000): 3775–83. http://dx.doi.org/10.1128/jb.182.13.3775-3783.2000.

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ABSTRACT Tn5397 is a conjugative transposon that was originally isolated from Clostridium difficile. Previous analysis had shown that the central region of Tn5397 was closely related to the conjugative transposon Tn916. However, in this work we obtained the DNA sequence of the ends of Tn5397 and showed that they are completely different to those of Tn916. Tn5397 did not contain theint and xis genes, which are required for the excision and integration of Tn916. Instead, the right end of Tn5397 contained a gene, tndX, that appears to encode a member of the large resolvase family of site-specific
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Shoemaker, Nadja B., Gui-Rong Wang, and Abigail A. Salyers. "Multiple Gene Products and Sequences Required for Excision of the Mobilizable Integrated BacteroidesElement NBU1." Journal of Bacteriology 182, no. 4 (2000): 928–36. http://dx.doi.org/10.1128/jb.182.4.928-936.2000.

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ABSTRACT NBU1 is an integrated 10.3-kbp Bacteroides element, which can excise and transfer to Bacteroides orEscherichia coli recipients, where it integrates into the recipient genome. NBU1 relies on large, >60-kbp, conjugative transposons for factors that trigger excision and for mobilization of the circular form to recipients. Previously, we showed that a single integrase gene, intN1, was necessary and sufficient for integration of NBU1 into its target site on the Bacteroidesor E. coli genome. We now show that an unexpectedly large region of NBU1 is required for excision. This region inclu
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Le Hello, Simon, François-Xavier Weill, Véronique Guibert, Karine Praud, Axel Cloeckaert, and Benoît Doublet. "Early Strains of Multidrug-Resistant Salmonella enterica Serovar Kentucky Sequence Type 198 from Southeast Asia Harbor Salmonella Genomic Island 1-J Variants with a Novel Insertion Sequence." Antimicrobial Agents and Chemotherapy 56, no. 10 (2012): 5096–102. http://dx.doi.org/10.1128/aac.00732-12.

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ABSTRACTSalmonellagenomic island 1 (SGI1) is a 43-kb integrative mobilizable element that harbors a great diversity of multidrug resistance gene clusters described in numerousSalmonella entericaserovars and also inProteus mirabilis. The majority of SGI1 variants contain an In104-derivative complex class 1 integron inserted between resolvase generesand open reading frame (ORF) S044 in SGI1. Recently, the international spread of ciprofloxacin-resistantS. entericaserovar Kentucky sequence type 198 (ST198) containing SGI1-K variants has been reported. A retrospective study was undertaken to charac
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Rivard, Nicolas, Malika Humbert, Kévin T. Huguet, et al. "Surface exclusion of IncC conjugative plasmids and their relatives." PLOS Genetics 20, no. 10 (2024): e1011442. http://dx.doi.org/10.1371/journal.pgen.1011442.

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The phenomenon of exclusion allows conjugative plasmids to selectively impede the entry of identical or related elements into their host cell to prevent the resulting instability. Entry exclusion blocks DNA translocation into the recipient cell, whereas surface exclusion destabilizes the mating pair. IncC conjugative plasmids largely contribute to the dissemination of antibiotic-resistance genes in Gammaproteobacteria. IncC plasmids are known to exert exclusion against their relatives, including IncC and IncA plasmids, yet the entry exclusion factor eexC alone does not account for the totality
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Anyanwu, Madubuike Umunna, Ishmael Festus Jaja, Charles Odilichukwu R. Okpala, Emmanuel Okechukwu Njoga, Nnenna Audrey Okafor, and James Wabwire Oguttu. "Mobile Colistin Resistance (mcr) Gene-Containing Organisms in Poultry Sector in Low- and Middle-Income Countries: Epidemiology, Characteristics, and One Health Control Strategies." Antibiotics 12, no. 7 (2023): 1117. http://dx.doi.org/10.3390/antibiotics12071117.

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Mobile colistin resistance (mcr) genes (mcr-1 to mcr-10) are plasmid-encoded genes that threaten the clinical utility of colistin (COL), one of the highest-priority critically important antibiotics (HP-CIAs) used to treat infections caused by multidrug-resistant and extensively drug-resistant bacteria in humans and animals. For more than six decades, COL has been used largely unregulated in the poultry sector in low- and middle-income countries (LMICs), and this has led to the development/spread of mcr gene-containing bacteria (MGCB). The prevalence rates of mcr-positive organisms from the pou
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DE FREITAS ORTIZ, MAURO, and ELGION LUCIO SILVA LORETO. "Thehobo-related elements in themelanogasterspecies group." Genetics Research 90, no. 3 (2008): 243–52. http://dx.doi.org/10.1017/s0016672308009312.

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SummaryThehobo-related sequences (hRSs) were considered as degenerate and inactive elements until recently, when one mobilizable copy was described. Using this sequence as the initial seed to search for homologous sequences in 12 availableDrosophilagenomes, in addition to searching for these sequences by PCR and Southern blot in nine other species, we found homologous sequences in every species of theDrosophila melanogasterspecies subgroup. Some evidence suggests that these non-autonomous sequences were kept mobilizable for at least 0·4 million years. Also, some very short sequences with minia
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Bonafede, M. E., L. L. Carias, and L. B. Rice. "Enterococcal transposon Tn5384: evolution of a composite transposon through cointegration of enterococcal and staphylococcal plasmids." Antimicrobial Agents and Chemotherapy 41, no. 9 (1997): 1854–58. http://dx.doi.org/10.1128/aac.41.9.1854.

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Mechanisms for the possible transfer of antimicrobial resistance genes between staphylococci and enterococci remain poorly defined. We have previously reported the transfer between Enterococcus faecalis strains of a multiresistance chromosomal element (beta-lactamase positive and resistance to erythromycin, gentamicin, mercuric chloride, streptomycin, and tetracycline) which we have tentatively designated Tn5385. Tn5385 is a composite of several smaller transposable elements, including Tn5384, a 26-kb composite transposon conferring resistance to erythromycin, gentamicin, and mercuric chloride
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Rékási, Márk, and T. Filep. "Effect of Microelement Loads on the Element Fractions of Soil and Plant Uptake." Agrokémia és Talajtan 55, no. 1 (2006): 213–22. http://dx.doi.org/10.1556/agrokem.55.2006.1.23.

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The effect of microelement loads was investigated on the mobile (1 M NH 4 NO 3 soluble), mobilizable (NH 4 -acetate + EDTA soluble) and pseudo total (cc. HNO 3 + H 2 O 2 ) element concentrations of a calcareous chernozem soil in a long-term field experiment. Salts of 13 microelements were applied on four levels: 0, 90, 270 and 810 kg/ha in the spring of 1991 prior to sowing maize. The relations between the mobile, mobilizable and pseudo total fractions of a heavy metal contaminated soil were defined and quantified. According to this, Ba, Mo, Sr and Se have the greatest mobility, while Cu, Ni a
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35

Osman, Ashraf S., and Malcolm D. Bolton. "A new design method for retaining walls in clay." Canadian Geotechnical Journal 41, no. 3 (2004): 451–66. http://dx.doi.org/10.1139/t04-003.

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Abstract (sommario):
Geotechnical design engineers used to rely on arbitrary rules and definitions of "factor of safety" on peak soil strength in limit analysis calculations. They used elastic stiffness for deformation calculations, but the selection of equivalent linear elastic models was always arbitrary. Therefore, there is a need for a simple unified design method that addresses the real nature of serviceability and collapse limits in soils, which always show a nonlinear and sometimes brittle response. An approach to this method can be based on a new application of the theory of plasticity accompanied by the i
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36

Wasels, François, Patrizia Spigaglia, Fabrizio Barbanti, and Paola Mastrantonio. "Clostridium difficile erm(B)-containing elements and the burden on the in vitro fitness." Journal of Medical Microbiology 62, no. 9 (2013): 1461–67. http://dx.doi.org/10.1099/jmm.0.057117-0.

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In Clostridium difficile, resistance to the macrolide-lincosamide-streptogramin B group of antibiotics generally relies on erm(B) genes. In this study, we investigated elements with a genetic organization different from Tn5398, the mobilizable non-conjugative element identified in C. difficile strain 630. Our results suggested that the elements most frequently found in strains isolated during the European surveillance study in 2005 were related to Tn6194, the conjugative transposon recently detected in different C. difficile types, including PCR-ribotype 027. We characterized a Tn6194-like and
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37

McCarron, M., A. Duttaroy, G. Doughty, and A. Chovnick. "Drosophila P element transposase induces male recombination additively and without a requirement for P element excision or insertion." Genetics 136, no. 3 (1994): 1013–23. http://dx.doi.org/10.1093/genetics/136.3.1013.

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Abstract P element dysgenesis-associated male recombination in Drosophila was examined with a selective system focused upon a section of the third chromosome divided into eight recombination segments. Tests compared crossing over in the presence of none, one and two doses of P(delta 2-3)(99B), a non-mobile transposase source, in the absence of a mobilizable P element target in the genome. In the presence of the P transposase source, and without a P element target, significant male recombination occurred in genomic regions physically separated from the P(delta 2-3) site. Using two doses of P(de
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38

Timmery, Sophie, Pauline Modrie, Olivier Minet, and Jacques Mahillon. "Plasmid Capture by the Bacillus thuringiensis Conjugative Plasmid pXO16." Journal of Bacteriology 191, no. 7 (2009): 2197–205. http://dx.doi.org/10.1128/jb.01700-08.

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Abstract (sommario):
ABSTRACTConjugation, mobilization, and retromobilization are three related mechanisms of horizontal gene transfer in bacteria. They have been extensively studied in gram-negative species, where retromobilization, the capture of DNA from a recipient by a donor cell, was shown to result from two successive steps: the transfer of the conjugative plasmid from the donor to the recipient followed by the retrotransfer of the mobilizable plasmid to the donor. This successive model was established for gram-negative bacteria but was lacking experimental data from the gram-positive counterparts. In the p
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39

Shoemaker, N. B., G. R. Wang, and A. A. Salyers. "NBU1, a mobilizable site-specific integrated element from Bacteroides spp., can integrate nonspecifically in Escherichia coli." Journal of bacteriology 178, no. 12 (1996): 3601–7. http://dx.doi.org/10.1128/jb.178.12.3601-3607.1996.

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40

Ceccarelli, Daniela, Aurélie Daccord, Mélissa René, and Vincent Burrus. "Identification of the Origin of Transfer (oriT) and a New Gene Required for Mobilization of the SXT/R391 Family of Integrating Conjugative Elements." Journal of Bacteriology 190, no. 15 (2008): 5328–38. http://dx.doi.org/10.1128/jb.00150-08.

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Abstract (sommario):
ABSTRACT Integrating conjugative elements (ICEs) are self-transmissible, mobile elements that are widespread among bacteria. Following their excision from the chromosome, ICEs transfer by conjugation, a process initiated by a single-stranded DNA break at a specific locus called the origin of transfer (oriT). The SXT/R391 family of ICEs includes SXTMO10, R391, and more than 25 related ICEs found in gammaproteobacteria. A previous study mapped the oriT locus of SXTMO10 to a 550-bp intergenic region between traD and s043. We suspected that this was not the correct oriT locus, because the identica
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41

Chanchaithong, Pattrarat, Vincent Perreten, and Sybille Schwendener. "Macrococcus canis contains recombinogenic methicillin resistance elements and the mecB plasmid found in Staphylococcus aureus." Journal of Antimicrobial Chemotherapy 74, no. 9 (2019): 2531–36. http://dx.doi.org/10.1093/jac/dkz260.

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Abstract (sommario):
Abstract Objectives To analyse the genetic context of mecB in two Macrococcus canis strains from dogs, compare the mecB-containing elements with those found in other Macrococcus and Staphylococcus species, and identify possible mobilizable mecB subunits. Methods Whole genomes of the M. canis strains Epi0076A and KM0218 were sequenced using next-generation sequencing technologies. Multiple PCRs and restriction analysis confirmed structures of mecB-containing elements, circularization and recombination of mecB subunits. Results Both M. canis strains contained novel composite pseudo (Ψ) staphyloc
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42

Schirova, I. A. "Text as an Element of Integrative Scientific Space." RUSSIAN JOURNAL OF LINGUISTICS 21, no. 1 (2017): 362–78. http://dx.doi.org/10.22363/2312-9182-2017-21-2-362-378.

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43

Cappuyns, Valérie. "A Critical Evaluation of Single Extractions from the SMT Program to Determine Trace Element Mobility in Sediments." Applied and Environmental Soil Science 2012 (2012): 1–15. http://dx.doi.org/10.1155/2012/672914.

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Abstract (sommario):
Two commonly applied single extractions procedures, namely extractions with ammonium-EDTA and acetic acid, were evaluated based on the analysis of 72 samples from alluvial sediments. For most trace elements (Cu, Zn, Cd, Ni, As, and Pb), a significant linear relationship could be established between their ammonium-EDTA or acetic acid extractable concentrations and their total concentrations, the organic carbon content, pH, and Fe , Al, and/or Ca content in the sediments. The scientific understanding of trace element partitioning in the complex soil-water system with these simple models is rathe
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44

Zhang, Qing Wen, Ying Jie Xu, Wei Hong Zhang, and Jun Wang. "Integrative Analysis of the Injection Molding Process and Mechanical Behavior of Plastic Part." Advanced Materials Research 705 (June 2013): 181–86. http://dx.doi.org/10.4028/www.scientific.net/amr.705.181.

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Abstract (sommario):
For parts made of plastics, injection molding is a common manufacturing process. Warpage and residual stress induced during the injection molding process have very important influences on the mechanical performance of injection products. In this paper, an integrative analysis of the injection molding process and mechanical performance of plastic parts is proposed. This integrative approach incorporates the effects of the manufacturing process in the mechanical simulation: (a) firstly, the finite element package MoldFlow is used to simulate the injection molding process and obtain the warpage a
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45

Vedantam, Gayatri, and David W. Hecht. "Isolation and Characterization of BTF-37: Chromosomal DNA Captured from Bacteroides fragilis That Confers Self-Transferability and Expresses a Pilus-Like Structure in Bacteroides spp. and Escherichia coli." Journal of Bacteriology 184, no. 3 (2002): 728–38. http://dx.doi.org/10.1128/jb.184.3.728-738.2002.

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Abstract (sommario):
ABSTRACT We report the isolation and preliminary characterization of BTF-37, a new 52-kb transfer factor isolated from Bacteroides fragilis clinical isolate LV23. BTF-37 was obtained by the capture of new DNA in the nonmobilizable Bacteroides-Escherichia coli shuttle vector pGAT400ΔBglII using a functional assay. BTF-37 is self-transferable within and from Bacteroides and also self-transfers in E. coli. Partial DNA sequencing, colony hybridization, and PCR revealed the presence of Tet element-specific sequences in BTF-37. In addition, Tn5520, a small mobilizable transposon that we described pr
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46

Araja, Diana, Angelika Krumina, Uldis Berkis, Zaiga Nora-Krukle, and Modra Murovska. "Integrative approach as a dormant element of integrated healthcare." International Journal of Integrated Care 23, S1 (2023): 760. http://dx.doi.org/10.5334/ijic.icic23628.

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Abstract (sommario):
This abstract focus on the integrative approach as a dormant element in the arsenal of integrated healthcare. The World Health Organization has noted that there are various challenges to implementing integrated health care, among others related to the coordination of care between sectors. This coordination includes the following strategies: Integration of health and social care, Intersectoral partnerships, and Integrating traditional and complementary medicine into modern health systems [1]. Each of these strategies has its own positive evidence-based examples and guidance on potential risks.
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47

Száková, J., J. Sysalová, and P. Tlustoš. "Particular aspects of environmental impact of potentially risk elements from airborne particulate matter." Plant, Soil and Environment 51, No. 8 (2011): 376–83. http://dx.doi.org/10.17221/3613-pse.

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Abstract (sommario):
Two simple experiments were carried out to demonstrate two possible ways of environmental impact of element contents in airborne particulate matter. In the first part of the experiment, the response of three rural dust samples applied into the soil were assessed in pot experiment to observe uptake of As, Cd, Pb, and Zn by aboveground biomass of oat (Avena sativa L.). Although the element contents in dust samples exceeded significantly total element contents in soil, the element content in plants was not affected by single-rate soil amendment with rural dust sample. Soil sorption capacity and n
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48

Creuzburg, Kristina, Bernd Köhler, Helena Hempel, Peter Schreier, Enno Jacobs, and Herbert Schmidt. "Genetic structure and chromosomal integration site of the cryptic prophage CP-1639 encoding Shiga toxin 1." Microbiology 151, no. 3 (2005): 941–50. http://dx.doi.org/10.1099/mic.0.27632-0.

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Abstract (sommario):
The sequence of 50 625 bp of chromosomal DNA derived from Shiga-toxin (Stx)-producing Escherichia coli (STEC) O111 : H− strain 1639/77 was determined. This DNA fragment contains the cryptic Stx1-encoding prophage CP-1639 and its flanking chromosomal regions. The genome of CP-1639 basically resembles that of lambdoid phages in structure, but contains three IS629 elements, one of which disrupts the gene of a tail fibre component. The prophage genome lacks parts of the recombination region including integrase and excisionase genes. Moreover, a capsid protein gene is absent. CP-1639 is closely ass
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49

Takács, Anita, Katalin Kovács, Gábor Halász, et al. "Improvement of the sequential extraction procedure based on supercritical CO2 and subcritical H2O solvents for the estimation of the environmentally mobile potentially toxic element fractions of sediments and soils." Agrokémia és Talajtan 67, no. 1 (2018): 35–48. http://dx.doi.org/10.1556/0088.2018.67.1.3.

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Abstract (sommario):
The estimation of environmental risk caused by pollution with potentially toxic elements (PTE) is usually carried out using the (3+1) step sequential extraction procedure suggested in 1993 by the Community Bureau of Reference (BCR). In the 1st step the water-soluble, exchangeable and carbonate-bound element content is extracted with acetic acid. In 2002 a fractionation procedure based on the application of supercritical CO2, subcritical H2O and of a mixture of subcritical H2O/CO2 was proposed, which allowed the water-soluble and carbonatebound element contents to be extracted separately from s
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50

Zhao, Sihui, and Kelly P. Williams. "Integrative Genetic Element That Reverses the Usual Target Gene Orientation." Journal of Bacteriology 184, no. 3 (2002): 859–60. http://dx.doi.org/10.1128/jb.184.3.859-860.2002.

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Abstract (sommario):
ABSTRACT A genetic element integrating site specifically into a prokaryotic gene usually carries a copy of the 3′ portion of that gene that restores the active gene even as the original is disrupted. A cryptic element in Mesorhizobium loti instead carries a copy of the 5′ end of the tRNA gene into which it integrated. This has implications for the evolution of new integrase-site combinations.
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