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Letteratura scientifica selezionata sul tema "Legionella detection"

Autore: Grafiati

Pubblicato: 4 giugno 2021

Ultima modifica: 1 febbraio 2022

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Articoli di riviste sul tema "Legionella detection"

Cloud, J. L., K. C. Carroll, P. Pixton, M. Erali e D. R. Hillyard. "Detection of Legionella Species in Respiratory Specimens Using PCR with Sequencing Confirmation". Journal of Clinical Microbiology 38, n. 5 (2000): 1709–12. http://dx.doi.org/10.1128/jcm.38.5.1709-1712.2000.

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Abstract (sommario):

Legionella spp. are a common cause of community-acquired respiratory tract infections and an occasional cause of nosocomial pneumonia. A PCR method for the detection of legionellae in respiratory samples was evaluated and was compared to culture. The procedure can be performed in 6 to 8 h with a commercially available DNA extraction kit (Qiagen, Valencia, Calif.) and by PCR with gel detection. PCR is performed with primers previously determined to amplify a 386-bp product within the 16S rRNA gene of Legionella pneumophila. We can specifically detect the clinically significant Legionella species including L. pneumophila, L. micdadei, L. longbeachae, L. bozemanii, L. feeleii, and L. dumoffii. The assay detects 10 fg (approximately two organisms) of legionella DNA in each PCR. Of 212 clinical specimens examined by culture, 100% of the culture-positive samples (31 of 31) were positive by this assay. By gel detection of amplification products, 12 of 181 culture-negative samples were positive forLegionella species by PCR, resulting in 93% specificity. Four of the 12 samples with discrepant results (culture negative, PCR positive) were confirmed to be positive for Legionellaspecies by sequencing of the amplicons. The legionella-specific PCR assay that is described demonstrates high sensitivity and high specificity for routine detection of legionellae in respiratory samples.

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Toplitsch, Daniela, Sabine Platzer, Romana Zehner, Stephanie Maitz, Franz Mascher e Clemens Kittinger. "Comparison of Updated Methods for Legionella Detection in Environmental Water Samples". International Journal of Environmental Research and Public Health 18, n. 10 (19 maggio 2021): 5436. http://dx.doi.org/10.3390/ijerph18105436.

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The difficulty of cultivation of Legionella spp. from water samples remains a strenuous task even for experienced laboratories. The long incubation periods for Legionellae make isolation difficult. In addition, the water samples themselves are often contaminated with accompanying microbial flora, and therefore require complex cultivation methods from diagnostic laboratories. In addition to the recent update of the standard culture method ISO 11731:2017, new strategies such as quantitative PCR (qPCR) are often discussed as alternatives or additions to conventional Legionella culture approaches. In this study, we compared ISO 11731:2017 with qPCR assays targeting Legionella spp., Legionella pneumophila, and Legionella pneumophila serogroup 1. In samples with a high burden of accompanying microbial flora, qPCR shows an excellent negative predictive value for Legionella pneumophila, thus making qPCR an excellent tool for pre-selection of negative samples prior to work-intensive culture methods. This and its low limit of detection make qPCR a diagnostic asset in Legionellosis outbreak investigations, where quick-risk assessments are essential, and are a useful method for monitoring risk sites.

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Fricker, E. J., e C. R. Fricker. "Detection of legionella spp. using a commercially available polymerase chain reaction test". Water Science and Technology 31, n. 5-6 (1 marzo 1995): 407–8. http://dx.doi.org/10.2166/wst.1995.0649.

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A commercial test kit (the EnviroAmp Legionella Kit) for the detection of legionellas using the polymerase chain reaction is compared with the standard culture methods for water samples. The EnviroAmp kit proved to be rapid and did not require great experience of molecular biological techniques. However as number of samples which tested negative with the standard culture, were positive with the kit; further research is needed to establish whether this due to the detection of dead or viable but non-culturable legionellas.

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Wellinghausen, Nele, Cathrin Frost e Reinhard Marre. "Detection of Legionellae in Hospital Water Samples by Quantitative Real-Time LightCycler PCR". Applied and Environmental Microbiology 67, n. 9 (1 settembre 2001): 3985–93. http://dx.doi.org/10.1128/aem.67.9.3985-3993.2001.

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ABSTRACT Contamination of hospital water systems with legionellae is a well-known cause of nosocomial legionellosis. We describe a new real-time LightCycler PCR assay for quantitative determination of legionellae in potable water samples. Primers that amplify both a 386-bp fragment of the 16S rRNA gene from Legionellaspp. and a specifically cloned fragment of the phage lambda, added to each sample as an internal inhibitor control, were used. The amplified products were detected by use of a dual-color hybridization probe assay design and quantified with external standards composed ofLegionella pneumophila genomic DNA. The PCR assay had a sensitivity of 1 fg of Legionella DNA (i.e., less than one Legionella organism) per assay and detected 44Legionella species and serogroups. Seventy-seven water samples from three hospitals were investigated by PCR and culture. The rates of detection of legionellae were 98.7% (76 of 77) by the PCR assay and 70.1% (54 of 77) by culture; PCR inhibitors were detected in one sample. The amounts of legionellae calculated from the PCR results were associated with the CFU detected by culture (r= 0.57; P < 0.001), but PCR results were mostly higher than the culture results. Since L. pneumophila is the main cause of legionellosis, we further developed a quantitativeL. pneumophila-specific PCR assay targeting the macrophage infectivity potentiator (mip) gene, which codes for an immunophilin of the FK506 binding protein family. All but one of the 16S rRNA gene PCR-positive water samples were also positive in the mip gene PCR, and the results of the two PCR assays were correlated. In conclusion, the newly developedLegionella genus-specific and L. pneumophila species-specific PCR assays proved to be valuable tools for investigation of Legionella contamination in potable water systems.

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Chambers, Stephen T., Sandy Slow, Amy Scott-Thomas e David R. Murdoch. "Legionellosis Caused by Non-Legionella pneumophila Species, with a Focus on Legionella longbeachae". Microorganisms 9, n. 2 (31 gennaio 2021): 291. http://dx.doi.org/10.3390/microorganisms9020291.

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Abstract (sommario):

Although known as causes of community-acquired pneumonia and Pontiac fever, the global burden of infection caused by Legionella species other than Legionella pneumophila is under-recognised. Non-L. pneumophila legionellae have a worldwide distribution, although common testing strategies for legionellosis favour detection of L. pneumophila over other Legionella species, leading to an inherent diagnostic bias and under-detection of cases. When systematically tested for in Australia and New Zealand, L. longbeachae was shown to be a leading cause of community-acquired pneumonia. Exposure to potting soils and compost is a particular risk for infection from L. longbeachae, and L. longbeachae may be better adapted to soil and composting plant material than other Legionella species. It is possible that the high rate of L. longbeachae reported in Australia and New Zealand is related to the composition of commercial potting soils which, unlike European products, contain pine bark and sawdust. Genetic studies have demonstrated that the Legionella genomes are highly plastic, with areas of the chromosome showing high levels of recombination as well as horizontal gene transfer both within and between species via plasmids. This, combined with various secretion systems and extensive effector repertoires that enable the bacterium to hijack host cell functions and resources, is instrumental in shaping its pathogenesis, survival and growth. Prevention of legionellosis is hampered by surveillance systems that are compromised by ascertainment bias, which limits commitment to an effective public health response. Current prevention strategies in Australia and New Zealand are directed at individual gardeners who use potting soils and compost. This consists of advice to avoid aerosols generated by the use of potting soils and use masks and gloves, but there is little evidence that this is effective. There is a need to better understand the epidemiology of L. longbeachae and other Legionella species in order to develop effective treatment and preventative strategies globally.

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Aoki, S., Y. Hirakata, Y. Miyazaki, K. Izumikawa, K. Yanagihara, K. Tomono, Y. Yamada, T. Tashiro, S. Kohno e S. Kamihira. "Detection of Legionella DNA by PCR of whole-blood samples in a mouse model". Journal of Medical Microbiology 52, n. 4 (1 aprile 2003): 325–29. http://dx.doi.org/10.1099/jmm.0.04999-0.

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A detection system for Legionella DNA in blood samples based on the PCR was developed and evaluated in A/J mice with experimentally induced Legionella pneumonia. Primers were designed to amplify a 106 bp DNA fragment of the 16S rRNA gene specific to Legionella species. The PCR system could detect clinically relevant Legionella species including Legionella pneumophila, Legionella micdadei, Legionella bozemanae, Legionella dumoffii, Legionella longbeachae, Legionella gormanii and Legionella jordanis. The sensitivity of the PCR system was 20 fg extracted DNA. In the mouse model, the blood PCR was compared with results obtained by PCR on bronchoalveolar lavage fluid (BALF) samples, cultures of blood and BALF and detection of Legionella urinary antigen. Blood PCR was positive until 8 days after infection, while BALF PCR became negative on day 4. These results indicate that PCR using blood samples may be a useful, convenient and non-invasive method for the diagnosis of Legionella pneumonia.

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Albalat, Guillermo Rodríguez, Begoña Bedrina Broch e Marisa Jiménez Bono. "Method Modification of the Legipid®Legionella Fast Detection Test Kit". Journal of AOAC INTERNATIONAL 97, n. 5 (1 settembre 2014): 1403–9. http://dx.doi.org/10.5740/jaoacint.14-029.

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Abstract Legipid®Legionella Fast Detection is a test based on combined magnetic immunocapture and enzyme-immunoassay (CEIA) for the detection of Legionella in water. The test is based on the use of anti-Legionella antibodies immobilized on magnetic microspheres. Target microorganism is preconcentrated by filtration. Immunomagnetic analysis is applied on these preconcentrated water samples in a final test portion of 9 mL. The test kit was certified by the AOAC Research Institute as Performance Tested MethodSM (PTM) No. 111101 in a PTM validation which certifies the performance claims of the test method in comparison to the ISO reference method 11731-1998 and the revision 11731-2004 “Water Quality: Detection and Enumeration of Legionella pneumophila” in potable water, industrial water, and waste water. The modification of this test kit has been approved. The modification includes increasing the target analyte from L. pneumophila to Legionella species and adding an optical reader to the test method. In this study, 71 strains of Legionella spp. other than L. pneumophila were tested to determine its reactivity with the kit based on CEIA. All the strains of Legionella spp. tested by the CEIA test were confirmed positive by reference standard method ISO 11731. This test (PTM 111101) has been modified to include a final optical reading. A methods comparison study was conducted to demonstrate the equivalence of this modification to the reference culture method. Two water matrixes were analyzed. Results show no statistically detectable difference between the test method and the reference culture method for the enumeration of Legionella spp. The relative level of detection was 93 CFU/volume examined (LOD50). For optical reading, the LOD was 40 CFU/volume examined and the LOQ was 60 CFU/volume examined. Results showed that the test Legipid Legionella Fast Detection is equivalent to the reference culture method for the enumeration of Legionella spp.

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Oshiro, Robin K., Teresa Picone e Betty H. Olson. "Modification of reagents in the EnviroAmp™ kit to increase recovery of Legionella organisms in water". Canadian Journal of Microbiology 40, n. 6 (1 giugno 1994): 495–99. http://dx.doi.org/10.1139/m94-080.

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Organisms of the bacterial genus Legionella, commonly found in aqueous reservoirs, have been associated with Legionnaires' disease (legionella pneumonia, caused by Legionella pneumophila) and Pontiac fever (nonpneumonic legionellosis). EnviroAmp™ Legionella sample preparation, polymerase chain reaction amplification, and detection kits (Perkin-Elmer Corp.) were developed for rapid detection of DNA from organisms of the genus Legionella and the species L. pneumophila from environmental water samples. The kits are based on molecular techniques incorporating polymerase chain reaction amplification and detection by reverse dot blot hybridization to particular genus and species probes. The manufacturer states that the EnviroAmp™ Legionella sample preparation, polymerase chain reaction amplification, and detection kits can detect approximately 100 Legionella organisms/mL (10 000 organisms/100 mL) in the original water sample. The sensitivity of the kits was increased to 0.1 colony-forming units/mL (10 colony-forming units/100 mL), at least for cultured organisms, by modifying the EnviroAmp™ Legionella sample preparation kit protocol. Data obtained in this study indicated that sample volume could be increased from 100 to 1000 mL (in the absence of interfering substances such as humic acid) and DNA extraction volume could be decreased from 2 to 0.5 mL to increase the ability of the kit to detect lower numbers of Legionella spp. or L. pneumophila per volume.Key words: Legionella, environment, water, EnviroAmp™ kit.

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Chang, Bin, Kanji Sugiyama, Toshitsugu Taguri, Junko Amemura-Maekawa, Fumiaki Kura e Haruo Watanabe. "Specific Detection of Viable Legionella Cells by Combined Use of Photoactivated Ethidium Monoazide and PCR/Real-Time PCR". Applied and Environmental Microbiology 75, n. 1 (31 ottobre 2008): 147–53. http://dx.doi.org/10.1128/aem.00604-08.

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ABSTRACT Legionella organisms are prevalent in manmade water systems and cause legionellosis in humans. A rapid detection method for viable Legionella cells combining ethidium monoazide (EMA) and PCR/real-time PCR was assessed. EMA could specifically intercalate and cleave the genomic DNA of heat- and chlorine-treated dead Legionella cells. The EMA-PCR assay clearly showed an amplified fragment specific for Legionella DNA from viable cells, but it could not do so for DNA from dead cells. The number of EMA-treated dead Legionella cells estimated by real-time PCR exhibited a 104- to 105-fold decrease compared to the number of dead Legionella cells without EMA treatment. Conversely, no significant difference in the numbers of EMA-treated and untreated viable Legionella cells was detected by the real-time PCR assay. The combined assay was also confirmed to be useful for specific detection of culturable Legionella cells from water samples obtained from spas. Therefore, the combined use of EMA and PCR/real-time PCR detects viable Legionella cells rapidly and specifically and may be useful in environmental surveillance for Legionella.

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Roll, Bruce M., e Roger S. Fujioka. "Detection of legionella bacteria in sewage by polymerase chain reaction and standard culture method". Water Science and Technology 31, n. 5-6 (1 marzo 1995): 409–16. http://dx.doi.org/10.2166/wst.1995.0650.

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Legionella bacteria are ubiquitous in environmental waters. Only a few species of Legionella , especially, L. pneumophila are pathogenic to humans and cause a sometimes fatal Legionnaires disease as well as a less fatal disease called Pontiac fever. The presence of Legionella in sewage and aerosolized sewage is the subject of this investigation because reuse of sewage may involve the exposure of people to aerosolization, the mode of transmission of Legionella bacteria. The objective of this study was to determine the prevalence of Legionella species and L. pneumophila in wastewater and their fate after various stages of treatment. The polymerase chain reaction (PCR) and standard culture method were utilized to detect Legionella species and L. pneumophila. PCR results indicated that Legionella species were present at levels > 103 cells / ml during all phases of sewage treatment including chlorinated effluents. Culture results indicated levels at least one log lower than seen with PCR. Legionella species were also recovered from air samples collected from secondary aeration basins at levels < 103 cells/ml. PCR was shown to be the most rapid and sensitive method for detecting Legionella in sewage.

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Più fonti

Tesi sul tema "Legionella detection"

Aurell, Helena. "Detection, identification and typing of clinical and environmental Legionella strains". Lyon 1, 2003. http://www.theses.fr/2003LYO10126.

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La légionellose est une pneumonie fréquente provoquée par l'inhalation d'un aérosol contaminé par Legionella. Pour la détection de L. Pneumophila dans l'eau, nous avons développé une méthode rapide et sensible utilisant la cytométrie en phase solide. Par la comparaison de la prévalence des espèces et des sérogroupes parmi des souches cliniques et environnementales, nous avons montré une répartition différente, ce qui suggère que certaines souches sont plus pathogènes. Afin d'identifier la source environnementale de la contamination, le typage par électrophorèse en champs pulsé (PFGE) est nécessaire. L'analyse des profils en PFGE a démontré que la souche endémique Paris avait une prévalence importante et était distribuée en France et en Europe. L'étude des souches sporadiques, épidémiques et endémiques par séquençage de gènes a montré que le fond génétique n'expliquait pas la différence de pathogénicité ; d'autres facteurs pourraient être impliqués.

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Strickhouser, Amanda. "Legionella pneumophila in Domestic Hot Water Systems: Evaluation of Detection Methods and Environmental Factors Affecting Survival". Thesis, Virginia Tech, 2007. http://hdl.handle.net/10919/36046.

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Legionella is the causative agent of Legionnairesâ disease which hospitalizes 8,000 to 18,000 people in the United States each year. The disease in transmitted through inhalation or aspiration of water containing the bacterium and can be acquired within the home. Studies have found that 0-37% of domestic water heaters contain Legionella, making household hot water systems a potential route of exposure. The objective of this research was to evaluate different methods for testing environmental samples for Legionella pneumophila and to analyze potable water conditions that affect survival of free living Legionella pneumophila in hot water tanks. Three heat pretreatment methods (50ÂºC for 30 minutes, 55ÂºC for 15 minutes, and 60ÂºC for 3 minutes) were not effective at recovering Legionella in this study. There was no statistically significant difference between the three acid pretreatment methods that were tested (pH 2.0 with a neutralizing solution, pH 2.2, and the CDC method). Six media (BCYE, DGVP, PCV, GPCV, CCVC, and GPVA) exhibited similar Legionella recovery, except for when high levels of non-Legionella organisms were present, in which case BCYE demonstrated lower recovery. When disinfectant was present, if sodium thiosulfate was not added before the disinfectant, Legionella recovery was lower. However, this result was not statistically significant for free chlorine until after 5 minutes. Pseudomonas aeruginosa (up to 67.5 cfu/ml) and pyocyanin (up to 9 mg/l) did not have an effect on Legionella recovery under the tested conditions. Environmental factors affecting survival of free living Legionella pneumophila in hot water tanks were also studied. After one day exposure in small-scale simulated water heaters at 55ÂºC, viable Legionella could not be recovered. At 44ÂºC, Legionellae were recovered after one day but only at very low levels after eight days. Between 23 and 37ÂºC, Legionella could survive longer than eight days. Copper (Cu2+) concentrations above 2160 ppb were found to be toxic to Legionella, but iron (Fe3+) between 1 and 2160 ppb did not affect survival. Above pH 11 survival was greatly reduced. No effect was observed between pH 5-10. When glass fiber filters were added to the reactors and they were seeded with tap water and sediment slurry, Legionellae were retained in 7 of 16 reactors for 327 days. The results of this work will assist in optimal identification of Legionella via microbial analysis of potable water samples, thereby assisting in prevention and diagnosis of factors contributing to Legionnairesâ disease, especially in settings with high-risk patients (e.g. hospitals). Water systems studying Legionella amplification in domestic hot water systems can use simulated or real distribution system sampling to reproduce and study factors that prevent or reduce Legionella growth and persistence.
Master of Science

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Bernander, Sverker. "Detection and epidemiologic subtyping of Legionella pneumophila using DNA-based molecular methods /". Stockholm, 2003. http://diss.kib.ki.se/2003/91-7349-745-2.

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Wilén, Charlotte. "Optimization of a method for detection of Legionella pneumophila in water samples". Thesis, Uppsala universitet, Institutionen för medicinsk cellbiologi, 2021. http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-450384.

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Legionella pneumophila is a bacterium which can be found in fresh water and causes Legionnaires’ disease, which can be deadly for humans depending on the condition of the infected individual. The bacterium is a gram-negative rod and can withstand severe conditions such as high temperature. Therefore, various treatments including heat and acid treatment are performed on the water to inhibit interfering microorganisms. However, to examine a larger volume of water, the water needs to pass through a filter, which can be very time consuming, and there are various variables that have a negative impact on the filtration speed. The aim of this study was to examine these variables and find the fastest setup for detection of L. pneumophila. To filtrate the water, a manifold with funnels, where you put the water, is used, and the manifold is connected to a pump. Under the funnels, steel frits are placed, and the filter is placed on the steel frits. To examine the fastest setup, different manifolds, pumps, filters, and settings were investigated by timing the water running through in the different settings. A new way of sterilization, that does not damage the steel frits was tested, and the recovery of bacteria was examined on the filters with the top filtration speed. In conclusion, the most efficient setup is the Cyclopore (GE Healthcare Life Sciences) filter, the pump from KNF and the manifold MBS1 (Whatman), and the new way of sterilizing should be used to reduce the damage of the steel frits.

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Peter, Aji. "Novel approaches for risk management of Legionella bacteria in domestic water systems". Thesis, Brunel University, 2018. http://bura.brunel.ac.uk/handle/2438/17140.

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Legionella pneumophila, the causative agent of Legionnaires' disease, is a water born pathogenic bacteria commonly found in natural and manmade water systems such as rivers, lakes, wet soil, hot and cold water storage systems (being able to survive at temperatures between 6-63 °C, and proliferating between 20-45 °C), showerheads, cooling towers and spa pools. The main pathway of exposure to Legionella is by inhaling the aerosols containing the microorganism. Legionnaires' disease can be fatal if not diagnosed and treated at the right time. Practical Legionella control starts with a risk assessment of the water system and followed by the regular monitoring and water sampling. UK Health and Safety Executive (HSE) have implemented strict legislations to protect the public from Legionnaires' disease. This research highlights and addresses three major data gaps identified in Legionella control and management strategy employed in the UK and worldwide; namely, (i) the underestimation of microbiological threat in current cold water storage sampling strategy, (ii) the inability of current qPCR diagnostic methods to detect live Legionella in water samples, and (iii) the lack of predictive 'risk management system' for Legionella control in domestic water systems. During my PhD, 15 relevant cold water storage tanks (selected from more than 6000 tanks surveyed at different sites located in different London Boroughs) were used to investigate the risk factors that contribute towards Legionella proliferation, and revealed serious shortcomings in the appropriateness of the water sample taken for regulatory testing. Secondly, molecular biology research was carried out to develop an accurate, reliable and rapid testing method for the detection and quantification of live Legionella using qPCR techniques. This was successfully achieved by extracting RNA from a Legionella lenticule, converting the RNA into cDNA and amplifying the cDNA using qPCR techniques. Finally, regular monitoring data from 120 London buildings (60 known to be Legionella positive and 60 known to be Legionella negative) was used to identify the possible risk factors contributing towards Legionella outbreaks. Data for these factors was then used to develop a predictive risk model for Legionella contamination using Principal Component Analysis (PCA). The model was validated with 66 new London buildings and 9 out of London buildings. The model showed 100% accuracy in predicting the risk of Legionella by distinguishing infected and non-infected sites in London as well as for the sites in out of London.

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Brooks, Teresa Jane. "A comparative study using polymerase chain reaction, cultivation and immuno-magnetic separation for detection, isolation, and identification of Legionella spp. in water and biofilm samples from groundwaters". Thesis, University of Ottawa (Canada), 2001. http://hdl.handle.net/10393/9452.

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This study was carried out to determine the frequency and levels of occurrence of legionellae in groundwaters from a variety of sources in U.S. and Canada. A limited number of water samples from cooling towers were also tested because municipally treated waters are considered to be the main sources of Legionella in artificial habitats such as cooling towers, air conditioners, whirlpools, hot tubs and plumbing systems (Krammer and Ford, 1994). Conventional procedures, including cultivation and polymerase chain reaction (PCR), were used to determine the presence of Legionella spp. in the water and biofilm samples during this study. A novel approach, using immuno-magnetic separation (IMS) in combination with cultivation or PCR, was also explored as an improved and rapid detection method for Legionella over conventional procedures. Because the IMS technique was continually being improved over the course of this study, all the samples received did not undergo the same procedure. (Abstract shortened by UMI.)

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Kahlisch, Leila Kathrin [Verfasser], e Manfred G. [Akademischer Betreuer] Höfle. "Molecular analyses of drinking water bacteria critical for human health issues - Distinction between live and dead species and high resolution in situ detection of pathogenic bacteria exemplified for Legionella pneumophila / Leila Kathrin Kahlisch ; Betreuer: Manfred G. Höfle". Braunschweig : Technische Universität Braunschweig, 2010. http://d-nb.info/117582724X/34.

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Bartie, Catheleen. "Evaluation of detection methods for Legionella in industrial cooling water systems". Thesis, 2002. http://hdl.handle.net/2263/29471.

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Please read the "Background" (p v) in the section 00front of this document Copyright 2002, University of Pretoria. All rights reserved. The copyright in this work vests in the University of Pretoria. No part of this work may be reproduced or transmitted in any form or by any means, without the prior written permission of the University of Pretoria. Please cite as follows: Bartie, C 2002, The life and career of the South African dramatric soprano Marita Napier, DPhil thesis, University of Pretoria, Pretoria, viewed yymmdd < http://upetd.up.ac.za/thesis/available/etd-11142007-125718 / >
Thesis (DPhil (Microbiology))--University of Pretoria, 2007.
Microbiology and Plant Pathology
unrestricted

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Singh, Tanusha. "Detection of legionella in dental unit waterlines using different techniques". Thesis, 2002. https://hdl.handle.net/10539/26362.

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A dissertation submitted to the Faculty of Health Sciences, University of the Witwatersrand, Johannesburg, in fulfilment of the requirements for the degree of Master of Science in Medicine. Johannesburg, 2002
Water stagnation in dental waterlines may cause contaminating organisms including Legionella and amoeba to multiply to potentially hazardous levels. The purpose of this study was to determine the presence of Legionella in the dental unit waterlines at the Dental Teaching Hospital, University of the Witwatersrand using different techniques
IT2019

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Goosen, Coenie. "Development of PCR-based detection assays for Legionella pneumophila in water". Diss., 2001. http://hdl.handle.net/2263/29284.

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Capitoli di libri sul tema "Legionella detection"

Lindsay, Diane S. J., William Abraham, Giles Edwards e R. W. A. Girdwood. "Detection of Legionella-Specific DNA in Serum". In Legionella, 216–20. Washington, DC, USA: ASM Press, 2014. http://dx.doi.org/10.1128/9781555817985.ch41.

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Rossmoore, Katalin, Leonard Rossmoore e Christine Cuthbert. "Method Development for Legionella Detection in Metalworking Fluids". In Legionella, 463–64. Washington, DC, USA: ASM Press, 2014. http://dx.doi.org/10.1128/9781555815660.ch111.

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Bartie, Catheleen, Fanus Venter e Louis Nel. "Legionella Detection from South African Cooling Water Systems". In Legionella, 284–90. Washington, DC, USA: ASM Press, 2014. http://dx.doi.org/10.1128/9781555817985.ch56.

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Franzin, Laura, Daniela Cabodi e Nicoletta Bonfrate. "Legionella Detection from Water Samples by Real-Time PCR". In Legionella, 446–48. Washington, DC, USA: ASM Press, 2014. http://dx.doi.org/10.1128/9781555815660.ch106.

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Kircher, Jörn, Alexander Kirchhoff e Arndt Rolfs. "Specific Detection of Legionella in Samples from Patients with Community-Acquired Pneumonia by PCR and a Colorimetric Detection System (Reverse Dot Blot)". In Legionella, 51–52. Washington, DC, USA: ASM Press, 2014. http://dx.doi.org/10.1128/9781555815660.ch13.

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Giglio, Steven, Paul T. Monis e Christopher P. Saint. "A Novel and Rapid Legionella Detection System for Water Analysis". In Legionella, 453–55. Washington, DC, USA: ASM Press, 2014. http://dx.doi.org/10.1128/9781555815660.ch108.

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Valster, Rinske, Bart Wullings, Stefan Voost, Geo Bakker, Hauke Smidt e Dick van der Kooij. "Detection and Identification of Free-Living Protozoa Present in Drinking Water". In Legionella, 427–30. Washington, DC, USA: ASM Press, 2014. http://dx.doi.org/10.1128/9781555815660.ch101.

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Diederen, Bram M. W., Caroline M. A. de Jong, Faïcal Marmouk, Jan A. J. W. Kluytmans, Marcel F. Peeters e Anneke van der Zee. "Detection of Legionella pneumophila DNA in Serum Samples from Patients with Legionnaires' Disease". In Legionella, 47–50. Washington, DC, USA: ASM Press, 2014. http://dx.doi.org/10.1128/9781555815660.ch12.

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Moore, Norman, e Deborah Gentile. "Evaluation of a Rapid Immunochromatographic Assay for Detection of Legionella pneumophila in Urine". In Legionella, 211–12. Washington, DC, USA: ASM Press, 2014. http://dx.doi.org/10.1128/9781555817985.ch39.

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Helbig, Jürgen H., Søren A. Uldum, P. Christian Lück e Timothy G. Harrison. "Detection of Legionella pneumophila Antigen in Urine Samples: Recognition of Serogroups and Monoclonal Subgroups". In Legionella, 204–6. Washington, DC, USA: ASM Press, 2014. http://dx.doi.org/10.1128/9781555817985.ch37.

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Atti di convegni sul tema "Legionella detection"

Roummieh, Y., P. Ghodous, P. Vanhems, C. Verdier e M. Dutra. "Collaborative environment for the detection and the follow-up of Legionella". In 2007 2nd International Conference on Digital Information Management. IEEE, 2007. http://dx.doi.org/10.1109/icdim.2007.4444292.

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Melaine, Feriei, e Maryam Tabrizian. "Functionalized gold nanoparticles for surface plasmon resonance detection of legionella pneumophila 16s rRNA". In 2016 IEEE SENSORS. IEEE, 2016. http://dx.doi.org/10.1109/icsens.2016.7808696.

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Tsao, Yu-Chia, Yi-Wen Yang, Woo-Hu Tsai e Tsong-Rong Yan. "Side-polished fiber immunosensor based on surface plasmon resonance for detection of Legionella pneumophila". In Biomedical Optics (BiOS) 2008, a cura di Israel Gannot. SPIE, 2008. http://dx.doi.org/10.1117/12.758498.

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"Detection of Legionella spp. and Other Pathogens in Water Systems of Nursing Homes and Spa Pools". In May 22-24, 2017 Kuala Lumpur (Malaysia). IIE, 2017. http://dx.doi.org/10.15242/iie.c0517009.

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Miller, R., e C. Martinez-Miller. "118. Real-Time PCR Method for Detecting and Enumerating Legionella from Environmental Samples". In AIHce 2002. AIHA, 2002. http://dx.doi.org/10.3320/1.2766033.

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Rapporti di organizzazioni sul tema "Legionella detection"

McDonough, E. A., C. P. Barrozo, K. L. Russell e D. Metzgar. A Multiplex PCR for Detection of Mycoplasma pneumoniae, Chlamydophila pneumoniae, Legionella pneumophila, and Bordetella pertussis in Clinical Specimens. Fort Belvoir, VA: Defense Technical Information Center, gennaio 2005. http://dx.doi.org/10.21236/ada432554.

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