Letteratura scientifica selezionata sul tema "Microfluidic method"

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Articoli di riviste sul tema "Microfluidic method"

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Liu, Jingji, Boyang Zhang, Yajun Zhang, and Yiqiang Fan. "Fluid control with hydrophobic pillars in paper-based microfluidics." Journal of Micromechanics and Microengineering 31, no. 12 (2021): 127002. http://dx.doi.org/10.1088/1361-6439/ac35c9.

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Abstract Paper-based microfluidics has been widely used in chemical and medical analysis applications. In the conventional paper-based microfluidic approach, fluid is propagating inside the porous structure, and the flow direction of the fluid propagation is usually controlled with the pre-defined hydrophobic barrier (e.g. wax). However, the fluid propagation velocity inside the paper-based microfluidic devices largely depends on the material properties of paper and fluid, the relative control method is rarely reported. In this study, a fluid propagation velocity control method is proposed for
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Kunjumon, Mekha, Libina Babu, and Aswathy Boss. "Microfluidics Relevant Approaches in Drug Delivery System Treatment of Cancer – A Review." INTERANTIONAL JOURNAL OF SCIENTIFIC RESEARCH IN ENGINEERING AND MANAGEMENT 08, no. 09 (2024): 1–5. http://dx.doi.org/10.55041/ijsrem37596.

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Microfluidics technology is a promising method for creating advanced drug delivery systems, particularly in cancer detection and treatment. These systems provide accurate, efficient, and user-friendly methods for cancer detection and treatment by examining small samples. Microfluidic devices can produce nanoparticles for medication administration and identify cancer-diagnostic variables from biological fluids. Due to their high sensitivity, high throughput, and low cost, microfluidics may be useful in cancer study. While not currently used in clinical settings, microfluidic systems are expecte
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LI, CHIYU, WANG LI, CHUNYANG GENG, HAIJUN REN, XIAOHUI YU, and BO LIU. "MICROFLUIDIC CHIP FOR CANCER CELL DETECTION AND DIAGNOSIS." Journal of Mechanics in Medicine and Biology 18, no. 01 (2018): 1830001. http://dx.doi.org/10.1142/s0219519418300016.

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Since cancer becomes the most deadly disease to our health, research on early detection on cancer cells is necessary for clinical treatment. The combination of microfluidic device with cell biology has shown a unique method for cancer cell research. In the present review, recent development on microfluidic chip for cancer cell detection and diagnosis will be addressed. Some typical microfluidic chips focussed on cancer cells and their advantages for different kinds of cancer cell detection and diagnosis will be listed, and the cell capture methods within the microfluidics will be simultaneousl
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BAI, BOFENG, ZHENGYUAN LUO, TIANJIAN LU, and FENG XU. "NUMERICAL SIMULATION OF CELL ADHESION AND DETACHMENT IN MICROFLUIDICS." Journal of Mechanics in Medicine and Biology 13, no. 01 (2013): 1350002. http://dx.doi.org/10.1142/s0219519413500024.

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Inspired by the complex biophysical processes of cell adhesion and detachment under blood flow in vivo, numerous novel microfluidic devices have been developed to manipulate, capture, and separate bio-particles for various applications, such as cell analysis and cell enumeration. However, the underlying physical mechanisms are yet unclear, which has limited the further development of microfluidic devices and point-of-care (POC) systems. Mathematical modeling is an enabling tool to study the physical mechanisms of biological processes for its relative simplicity, low cost, and high efficiency.
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Xi, Wang, Fang Kong, Joo Chuan Yeo, et al. "Soft tubular microfluidics for 2D and 3D applications." Proceedings of the National Academy of Sciences 114, no. 40 (2017): 10590–95. http://dx.doi.org/10.1073/pnas.1712195114.

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Microfluidics has been the key component for many applications, including biomedical devices, chemical processors, microactuators, and even wearable devices. This technology relies on soft lithography fabrication which requires cleanroom facilities. Although popular, this method is expensive and labor-intensive. Furthermore, current conventional microfluidic chips precludes reconfiguration, making reiterations in design very time-consuming and costly. To address these intrinsic drawbacks of microfabrication, we present an alternative solution for the rapid prototyping of microfluidic elements
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Switalla, Ander, Lael Wentland, and Elain Fu. "3D printing-based microfluidic devices in fabric." Journal of Micromechanics and Microengineering 33, no. 2 (2023): 027001. http://dx.doi.org/10.1088/1361-6439/acaff1.

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Abstract Fabric-based microfluidics is a growing sub-field of porous materials-based microfluidics. 3D printing has been demonstrated as a useful fabrication method for open channel microfluidic devices, and also in the context of porous substates such as cellulose. In the current report, we describe a straightforward method for 3D printing fabric-based microfluidic devices. We demonstrate the ability to create both full and partial barriers in fabric, characterizing minimum channel and barrier widths, as well as reproducibility of the method using the metric of flow time repeatability through
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Prajitna, Stefanus H., Christian Harito, and Brian Yuliarto. "Cost-Effective Manufacturing of Microfluidics Through the Utilization of Direct Ink Writing." Emerging Science Journal 9, no. 1 (2025): 1–11. https://doi.org/10.28991/esj-2025-09-01-01.

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Microfluidics is essential for precise manipulation of fluids in small channels. However, conventional manufacturing processes for microfluidic devices are expensive, time-consuming, and require specialized equipment in a clean room. While recent studies have improved the cost-effectiveness of this device, there is still a need for further advancement in cost efficiency. Therefore, this study aimed to develop a custom-built direct-ink writing (DIW) printer for manufacturing microfluidic devices that is more affordable. Custom-built DIW directly printed microfluidic channels onto microscope sli
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Yip, Hon Ming, John C. S. Li, Kai Xie, et al. "Automated Long-Term Monitoring of Parallel Microfluidic Operations Applying a Machine Vision-Assisted Positioning Method." Scientific World Journal 2014 (2014): 1–14. http://dx.doi.org/10.1155/2014/608184.

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As microfluidics has been applied extensively in many cell and biochemical applications, monitoring the related processes is an important requirement. In this work, we design and fabricate a high-throughput microfluidic device which contains 32 microchambers to perform automated parallel microfluidic operations and monitoring on an automated stage of a microscope. Images are captured at multiple spots on the device during the operations for monitoring samples in microchambers in parallel; yet the device positions may vary at different time points throughout operations as the device moves back
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Soitu, Cristian, Alexander Feuerborn, Cyril Deroy, Alfonso A. Castrejón-Pita, Peter R. Cook, and Edmond J. Walsh. "Raising fluid walls around living cells." Science Advances 5, no. 6 (2019): eaav8002. http://dx.doi.org/10.1126/sciadv.aav8002.

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An effective transformation of the cell culture dishes that biologists use every day into microfluidic devices would open many avenues for miniaturizing cell-based workflows. In this article, we report a simple method for creating microfluidic arrangements around cells already growing on the surface of standard petri dishes, using the interface between immiscible fluids as a “building material.” Conventional dishes are repurposed into sophisticated microfluidic devices by reshaping, on demand, the fluid structures around living cells. Moreover, these microfluidic arrangements can be further re
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Hamad, Eyad M., Ahmed Albagdady, Samer Al-Gharabli, et al. "Optimizing Rapid Prototype Development Through Femtosecond Laser Ablation and Finite Element Method Simulation for Enhanced Separation in Microfluidics." Journal of Nanofluids 12, no. 7 (2023): 1868–79. http://dx.doi.org/10.1166/jon.2023.2102.

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In recent years, microfluidic systems have emerged as promising tools for blood separation and analysis. However, conventional methods for prototyping microfluidic systems can be slow and expensive. In this study, we present a novel approach to rapid prototyping that combines femtosecond laser ablation and finite element method (FEM) simulation. The optimization of the prototyping process was achieved through systematic characterization of the laser ablation process and the application of FEM simulation to predict the flow behavior of the microfluidic devices. Using a dean-coupled inertial flo
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Tesi sul tema "Microfluidic method"

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Nguyen, Khanh H. (Khanh Huy). "Hot embossing as a method for rapid prototyping microfluidic devices." Thesis, Massachusetts Institute of Technology, 2013. http://hdl.handle.net/1721.1/85789.

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Thesis: M. Eng. in Manufacturing, Massachusetts Institute of Technology, Department of Mechanical Engineering, 2013.<br>Title as it appears in Degrees awarded program, September 17, 2003: Design and analysis of a hot embossing machine and the effects of toolware and accuracy of resin replication of high aspect ratio microfluidic features Cataloged from PDF version of thesis.<br>Includes bibliographical references (pages 132-135).<br>Hot embossing is a growing technology proven to be capable of reproducing micro-scale features on thermoplastics and can be an effective process for rapid prototyp
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Lustrino, Michelle E. (Michelle Elizabeth). "The development of an innovative bonding method for microfluidic applications." Thesis, Massachusetts Institute of Technology, 2011. http://hdl.handle.net/1721.1/67622.

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Thesis (S.M.)--Massachusetts Institute of Technology, Dept. of Mechanical Engineering, 2011.<br>Cataloged from PDF version of thesis.<br>Includes bibliographical references (p. 145-149).<br>The field of microfluidics has powerful applications in low-cost healthcare diagnostics, DNA analysis, and fuel cells, among others. As the field moves towards commercialization, the ability to robustly manufacture these devices at low cost is becoming more important. One of the many challenges in microfluidic manufacturing is the reliable sealing of the microfluidic chips once the channels have been genera
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Wu, Jun, and 吴隽. "Drug delivery devices fabricated by microfluidic method and their applications in long-term antimicrobial therapy." Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 2013. http://hdl.handle.net/10722/198816.

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Controlled drug delivery devices provide numerous advantages such as reduced side effects, higher therapeutic efficiency and improved patient compliance. Biodegradable polymer has become the most important material for controlled drug delivery device because of the excellent biocompatibility and tunable physicochemical properties. Biodegradable polymeric drug delivery devices are usually processed into various types of micro-particles due to the ease of fabrication and administration. However, controlling the drug release kinetics of these microparticles is still a challenge. One important rea
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PENNELLA, FRANCESCO. "Analysis of microscale flows in tissue engineering systems and microfluidic devices." Doctoral thesis, Politecnico di Torino, 2013. http://hdl.handle.net/11583/2514479.

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The doctoral research summarized in this thesis has focused on the study of microflows in Tissue Engineering (TE) scaffolds and microdevices. The thesis is organized in two parts. In the first part, the properties influencing mass transport through scaffold are investigated both experimentally and in silico. In detail: (1) an acoustic measurement system suitable for the evaluation of TE porous scaffolds and based on a single (pressure) transducer is developed; (2) realistic models of irregular porous scaffolds were reconstructed from micro-CT images and fluid transport through them is simulate
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Jeon, Jessie Sungyun. "3D cyclic olefin copolymer (COC) microfluidic chip fabrication using hot embossing method for cell culture platform." Thesis, Massachusetts Institute of Technology, 2010. http://hdl.handle.net/1721.1/61871.

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Thesis (S.M.)--Massachusetts Institute of Technology, Dept. of Mechanical Engineering, 2010.<br>Cataloged from PDF version of thesis.<br>Includes bibliographical references (p. 48-51).<br>A microfluidic system has been developed for studying the factors inducing different responses of cells in vascular system using a three-dimensional microenvironment. The devices have been transferred from PDMS to a platform in cyclic olefin copolymer (COC) which has advantages in terms of hydrophobicity, production by the more commercially-viable hot embossing technique, and amenability to surface treatments
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Winer, Michael Hubert. "A three-dimensional (3D) defocusing-based particle tracking method and applications to inertial focusing in microfluidic devices." Thesis, University of British Columbia, 2014. http://hdl.handle.net/2429/50194.

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Three-dimensional analysis of particles in flows within microfluidic devices is a necessary technique in the majority of current microfluidics research. One method that allows for accurate determination of particle positions in channels is defocusing-based optical detection. This thesis investigates the use of the defocusing method for particles ranging in size from 2-18 μm without the use of a three-hole aperture. Using a calibration-based analysis motivated by previous work, we were able to relate the particle position in space to its apparent size in an image. This defocusing method was the
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Othman, Rahimah. "Production of functional pharmaceutical nano/micro-particles by solvent displacement method using advanced micro-engineered dispersion devices." Thesis, Loughborough University, 2016. https://dspace.lboro.ac.uk/2134/22905.

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The rapid advancement of drug delivery systems (DDS) has raised the possibility of using functional engineered nano/micro-particles as drug carriers for the administration of active pharmaceutical ingredients (APIs) to the affected area. The major goals in designing these functional particles are to control the particle size, the surface properties and the pharmacologically active agents release in order to achieve the site-specification of the drug at the therapeutically optimal rate and dose regimen. Two different equipment (i.e. glass capillary microfluidic device and micro-engineered membr
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Murali, Divya. "A Sampling Method for the Reduction of Power Consumption in Battery Operated UHF Receivers." University of Akron / OhioLINK, 2008. http://rave.ohiolink.edu/etdc/view?acc_num=akron1220634056.

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Duford, David. "Instrumentation, fabrication techniques and method development for sample introduction, preparation and extraction on centrifugal microfluidic devices in motion." Thesis, McGill University, 2012. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=110441.

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A growing number of pollutants are being shown to have a large environmental and health impact resulting in stricter legislative limits. Increased environmental monitoring is forcing analytical chemists to consider automating and miniaturizing current standard methods. Instrumentation and sample handling techniques for centrifugal microfluidic devices in motion have been developed with the objective of integrating multi-step reactions into a single device for the analysis of environmental solid samples.In order to study and optimize centrifugal microfluidic devices in motion, motorized stage
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Kim, Ho Jun. "Theoretical and numerical studies of chaotic mixing." Diss., Texas A&M University, 2008. http://hdl.handle.net/1969.1/85940.

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Theoretical and numerical studies of chaotic mixing are performed to circumvent the difficulties of efficient mixing, which come from the lack of turbulence in microfluidic devices. In order to carry out efficient and accurate parametric studies and to identify a fully chaotic state, a spectral element algorithm for solution of the incompressible Navier-Stokes and species transport equations is developed. Using Taylor series expansions in time marching, the new algorithm employs an algebraic factorization scheme on multi-dimensional staggered spectral element grids, and extends classical confo
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Libri sul tema "Microfluidic method"

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Lu, Chang, and Scott S. Verbridge, eds. Microfluidic Methods for Molecular Biology. Springer International Publishing, 2016. http://dx.doi.org/10.1007/978-3-319-30019-1.

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Bontoux, Nathalie, Luce Dauphinot, and Marie-Claude Potier. Unravelling single cell genomics: Micro and nanotools. RSC Pub., 2010.

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Li, Xiujun, and Zhou Yu. Microfluidic devices for biomedical applications. Woodhead Publishing, 2013.

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Berthier, Jean. Microdrops and digital microfluidics. William Andrew Pub., 2008.

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Bontoux, Nathalie, Luce Dauphinot, and Marie-Claude Potier. Unravelling single cell genomics: Micro and nanotools. RSC Pub., 2010.

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D, Zahn Jeffrey, ed. Methods in bioengineering: Biomicrofabrication and biomicrofluidics. Artech House, 2010.

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Kilian, Dill, Liu Robin Hui, and Grodzinski Piotr, eds. Microarrays: Preparation, microfluidics, detection methods, and biological applications. Springer, 2009.

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Krishnendu, Chakrabarty, and Zeng Jun, eds. Design automation methods and tools for microfluidics-based biochips. Springer, 2006.

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1982-, Xu Tao, ed. Digital microfluidic biochips: Design automation and optimization. Taylor & Francis, 2010.

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D, Zahn Jeffrey, ed. Methods in bioengineering: Biomicrofabrication and biomicrofluidics. Artech House, 2010.

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Capitoli di libri sul tema "Microfluidic method"

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Shao, Chenren, and Don L. DeVoe. "Measuring Microchannel Electroosmotic Mobility and Zeta Potential by the Current Monitoring Method." In Microfluidic Diagnostics. Humana Press, 2012. http://dx.doi.org/10.1007/978-1-62703-134-9_4.

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Occhetta, Paola, Emilia Biffi, and Marco Rasponi. "A Reliable Reversible Bonding Method for Perfused Microfluidic Devices." In Neuromethods. Springer New York, 2015. http://dx.doi.org/10.1007/978-1-4939-2510-0_2.

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Conde, Alvaro J., Ieva Keraite, Nicholas R. Leslie, and Maïwenn Kersaudy-Kerhoas. "Microfluidic Acoustic Method for High Yield Extraction of Cell-Free DNA in Low-Volume Plasma Samples." In Microfluidic Systems for Cancer Diagnosis. Springer US, 2023. http://dx.doi.org/10.1007/978-1-0716-3271-0_11.

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Jiménez-Torres, José A., David J. Beebe, and Kyung E. Sung. "A Microfluidic Method to Mimic Luminal Structures in the Tumor Microenvironment." In Methods in Molecular Biology. Springer New York, 2016. http://dx.doi.org/10.1007/978-1-4939-3801-8_5.

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Lin, Ching-Hui, Hao-Chen Chang, Don-Ching Lee, Ing-Ming Chiu, and Chia-Hsien Hsu. "Enzyme-Free Dissociation of Neurospheres by a Microfluidic Chip-Based Method." In Methods in Molecular Biology. Springer New York, 2016. http://dx.doi.org/10.1007/7651_2016_348.

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Rahul, R., V. Aishwarya, Nikhil Prasad, R. S. Mini, and S. Kumar Ranjith. "Design and Development of Thermoplastic Microfluidic Device for Argentometric Mohr Method." In Fluid Mechanics and Fluid Power, Volume 6. Springer Nature Singapore, 2024. http://dx.doi.org/10.1007/978-981-99-5755-2_19.

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Lee, Nae Yoon, Masumi Yamada, and Minoru Seki. "Improved Sample Injection Method Adapting Hydrophobic Passive Valve Systems for Microfluidic Devices." In Micro Total Analysis Systems 2002. Springer Netherlands, 2002. http://dx.doi.org/10.1007/978-94-010-0504-3_22.

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Yusro, Muhammad. "Emerging Potential on Laser Engraving Method in Fabricating Mold for Microfluidic Technology." In Proceedings of the 2nd International Conference on Electronics, Biomedical Engineering, and Health Informatics. Springer Nature Singapore, 2022. http://dx.doi.org/10.1007/978-981-19-1804-9_16.

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Geertz, Marcel, Sylvie Rockel, and Sebastian J. Maerkl. "A High-Throughput Microfluidic Method for Generating and Characterizing Transcription Factor Mutant Libraries." In Methods in Molecular Biology. Humana Press, 2011. http://dx.doi.org/10.1007/978-1-61779-412-4_6.

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Lin, Ching-Hui, Hao-Chen Chang, Don-Ching Lee, Ing-Ming Chiu, and Chia-Hsien Hsu. "Erratum to: Enzyme-Free Dissociation of Neurospheres by a Microfluidic Chip-Based Method." In Methods in Molecular Biology. Springer New York, 2016. http://dx.doi.org/10.1007/978-1-4939-6550-2_327.

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Atti di convegni sul tema "Microfluidic method"

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N, Nirmala, and Gracia Nirmala Rani D. "Enhancing Digital Microfluidic Biochip Operations with Scheduling Interval Method." In 2025 38th International Conference on VLSI Design and 2025 24th International Conference on Embedded Systems (VLSID). IEEE, 2025. https://doi.org/10.1109/vlsid64188.2025.00109.

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N, Nirmala, Harishna S, and Gracia Nirmala Rani D. "An Optimization Algorithm for Digital Microfluidic Biochip Performance Using Interval Method." In 2024 International Conference on Smart Electronics and Communication Systems (ISENSE). IEEE, 2024. https://doi.org/10.1109/isense63713.2024.10872388.

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Galambos, Paul, and Conrad James. "Surface Micromachined Microfluidics: Example Microsystems, Challenges and Opportunities." In ASME 2005 Pacific Rim Technical Conference and Exhibition on Integration and Packaging of MEMS, NEMS, and Electronic Systems collocated with the ASME 2005 Heat Transfer Summer Conference. ASMEDC, 2005. http://dx.doi.org/10.1115/ipack2005-73491.

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A variety of fabrication techniques have been used to make microfluidic microsystems: bulk etching in silicon and glass, plastic molding and machining, and PDMS (silicone) casting. Surprisingly the most widely used method of integrated circuit (IC) fabrication (surface micromachining — SMM) has not been extensively utilized in microfluidics despite its wide use in MEMS. There are economic reasons that SMM is not often used in microfluidics; high infrastructure and start-up costs and relatively long fabrication times: and there are technical reasons; packaging difficulties, dominance of surface
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Dunning, Peter D., Pierre E. Sullivan, and Michael J. Schertzer. "Method for Characterization of Passive Mechanical Filtration of Particles in Digital Microfluidic Devices." In ASME 2014 International Mechanical Engineering Congress and Exposition. American Society of Mechanical Engineers, 2014. http://dx.doi.org/10.1115/imece2014-38875.

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The ability to remove unbound biological material from a reaction site has applications in many biological protocols, such as those used to detect pathogens and biomarkers. One specific application where washing is critical is the Enzyme-Linked ImmunoSorbent Assay (ELISA). This protocol requires multiple washing steps to remove multiple reagents from a reaction site. Previous work has suggested that a passive mechanical comb filter can be used to wash particles in digital microfluidic devices. A method for the characterization of passive mechanical filtration of particles in Digital MicroFluid
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Dou, James, Lu Chen, Rakesh Nayyar, and Stewart Aitchison. "A microfluidic based optical particle detection method." In SPIE BiOS, edited by Robert J. Nordstrom and Gerard L. Coté. SPIE, 2012. http://dx.doi.org/10.1117/12.905049.

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Pu, J., R. Sochol, Y. Jiang, and L. Lin. "Microfluidic channels fabricated using a lithography-free method." In TRANSDUCERS 2011 - 2011 16th International Solid-State Sensors, Actuators and Microsystems Conference. IEEE, 2011. http://dx.doi.org/10.1109/transducers.2011.5969563.

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Kim, I., T. An, W. Choi, C. S. Kim, H. J. Cha, and G. Lim. "Immobilization method of escherichia coli for microfluidic application." In 2013 Transducers & Eurosensors XXVII: The 17th International Conference on Solid-State Sensors, Actuators and Microsystems (TRANSDUCERS & EUROSENSORS XXVII). IEEE, 2013. http://dx.doi.org/10.1109/transducers.2013.6626837.

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Lai, Siyi, Yeny Hudiono, Ly J. Lee, Sylvia Daunert, and Marc J. Madou. "Novel bonding method for polymer-based microfluidic platforms." In Micromachining and Microfabrication, edited by Jean Michel Karam and John A. Yasaitis. SPIE, 2001. http://dx.doi.org/10.1117/12.442956.

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Singha, Kamalesh, Tuhina Samanta, Hafizur Rahaman, and Parthasarathi Dasguptay. "Method of droplet routing in digital microfluidic biochip." In 2010 IEEE/ASME International Conference on Mechatronic and Embedded Systems and Applications (MESA). IEEE, 2010. http://dx.doi.org/10.1109/mesa.2010.5552059.

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Chen, Xiaoming, Yukun Ren, Likai Hou, Tianyi Jiang, and Hongyuan Jiang. "Fluid Mixing Using Induced Charge Electro-Osmotic Transverse Flow Actuated by Asymmetrical Driving Electrode Sequence." In ASME 2019 6th International Conference on Micro/Nanoscale Heat and Mass Transfer. American Society of Mechanical Engineers, 2019. http://dx.doi.org/10.1115/mnhmt2019-4181.

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Abstract Microfluid mixing is an essential process in chemical analysis, drug test, and nanoparticle synthesis. Induced charge electro-osmosis (ICEO) has good capability in microfluid mixing for its reconfigurable vortex profile. We found experimentally ICEO transverse flow induced by the asymmetrical driving electrode has a good performance in disturbing the interface of two fluids. Encouraged by these aspects, we proposed a micromixer using ICEO transverse flows actuated by the asymmetrical driving electrode sequence to mix microfluids. We established a simulation model to investigate the ev
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Rapporti di organizzazioni sul tema "Microfluidic method"

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Chailapakul, Orawon. Novelty in Analytical Chemistry for Innovation of Detection. Chulalongkorn University, 2017. https://doi.org/10.58837/chula.res.2017.19.

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Analytical chemistry is the one of the most importance not only to all branches of chemistry but also to all the biological sciences, to engineering, and, more recently, medicine, public health, food, environment and the supply of energy in all forms. Therefore, the developments of novel detection methods play an important role to obtain both qualitative analysis and quantification of the chemical or biomolecule components of natural and artificial materials. This work has been separated into 3 groups for finishing the novelty in detection methods. First, novel nanomaterials-based or nanocompo
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Yao, Jennifer, Shalini Tripathi, Eugene Ilton, et al. Corrosion of U233-Doped Uranium Oxide using Microfluidics Methods. Office of Scientific and Technical Information (OSTI), 2022. http://dx.doi.org/10.2172/1908674.

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