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Letteratura scientifica selezionata sul tema "PCR-RFLP"

Autore: Grafiati

Pubblicato: 4 giugno 2021

Ultima modifica: 25 luglio 2025

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Articoli di riviste sul tema "PCR-RFLP"

Karaca, Mehmet, Ayse Gul Ince, Saadet Tugrul Ay, Kenan Turgut, and Ahmet Naci Onus. "PCR-RFLP and DAMD-PCR genotyping forSalviaspecies." Journal of the Science of Food and Agriculture 88, no. 14 (2008): 2508–16. http://dx.doi.org/10.1002/jsfa.3372.

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VICTOIR, K., A. L. BAÑULS, J. AREVALO, et al. "The gp63 gene locus, a target for genetic characterization of Leishmania belonging to subgenus Viannia." Parasitology 117, no. 1 (1998): 1–13. http://dx.doi.org/10.1017/s0031182098002789.

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In the present study the gp63 gene locus was used as a target for genetic characterization of Leishmania parasites by 2 methods: (i) RFLP analysis with several restriction enzymes (gp63–RFLP), and (ii) intra-genic PCR amplification coupled with restriction analysis (PCR–RFLP). Both methods were applied to a large number of natural isolates belonging to 4 species of the subgenus Viannia, namely L. (V.) braziliensis, L. (V.) peruviana, L. (V.) guyanensis and L. (V.) lainsoni: reference stocks of subgenus Leishmania were included as outgroups. Multilocus isoenzyme typing (MLEE) was used as a refe

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Hazlianda, Cut, Kamaliah Muis, and Isma Lubis. "A Comparative Study of Polymerase Chain Reaction-Restriction Fragment Length Polymorphism and Fungal Culture for the Evaluation of Fungal Species in Patients with Tinea Cruris." Open Access Macedonian Journal of Medical Sciences 5, no. 7 (2017): 844–47. http://dx.doi.org/10.3889/oamjms.2017.197.

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BACKGROUND: Tinea cruris is the second most common dermatophytosis in the world and the most common in Indonesia. The conventional laboratory tests for dermatophyte infection are slow and less specific. Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP) is a PCR method with the addition of enzyme after amplification, therefore enabling for more specific results.AIM: This study aimed to find whether the PCR-RFLP test could yield the same fungal species result as a fungal culture.METHODS: The specimens were skin scrapings from thirty-one patients suspected tinea cruris

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Bouzouita, Imen, Henda Draoui, Samia Mahdhi, Leila Essalah, and Leila Slim Saidi. "Evaluation of PCR pncA-restriction fragment length polymorphism and PCR amplification of genomic regions of difference for the identification of M. bovis strains in lymph nodes cultures." African Health Sciences 21, no. 3 (2021): 985–89. http://dx.doi.org/10.4314/ahs.v21i3.4.

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Background: A rapid accurate identification of Mycobacterium bovis is essential for surveillance purposes. Objectives: A PCR pncA-Restriction Fragment Length Polymorphism (RFLP) and a multiplex PCR based on the detection of 3 regions of difference (RD-PCR): RD9, RD4 and RD1 were evaluated for the identification of M. bovis in lymph nodes cultures, in Tunisia, during 2013-2015. Methods: Eighty-two M. tuberculosis complex strains were identified using the biochemical tests, GenoType MTBC assay, PCR pncA-RFLP and RD-PCR. Results: The PCR pncA-RFLP showed that 54 M. bovis strains

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Khayyira, Amalia Sitti, Viktoria Mardhika Estepane, and Amarila Malik. "RAPID PCR–BASED DETECTION OPTIMIZATION OF PORCINE DNA IN GELATIN CAPSULE SHELL." International Journal of Applied Pharmaceutics 10, no. 6 (2018): 217. http://dx.doi.org/10.22159/ijap.2018v10i6.29346.

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Objective: This study was conducted to optimize the genomic deoxyribonucleic acid (DNA) based molecular detection of gelatin derived from porcine by performing polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) and duplex PCR method employing cyt B gene.Methods: Optimization was carried out for DNA extraction, PCR conditions, and the sensitivity of the PCR-RFLP method. Due to the very low DNA trace in gelatin after the various manufacturing process, the extraction was optimized to obtain sufficient DNA which was visible on the agarose gel. PCR-RFLP was carried out us

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Andrini, Fauzia, Imam Supardi, Sunarjati Sudigdoadi, and Sadeli Masria. "Detection of Staphylococcus aureus’s Strain Similarity on Surgical Ward Nurses’s Hand and Nose and Post Operative Wound Infection Using Coa Gene Through PCR-RFLP Method." Jurnal Ilmu Kedokteran 4, no. 2 (2017): 116. http://dx.doi.org/10.26891/jik.v4i2.2010.116-122.

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Staphylococcus aureus (S.aureus) remains to be the most important cause of post operative wound infection. Nursescould become reservoirs to transmit S.aureus through contaminated hands transiently, or through colonized nose.Strain polymorphism could be determined by Restriction Fragment Length Polymorphism (RFLP), using coa gene andrestriction endonuclease enzyme Alu1. There were 30 isolates of S.aureus’s infection, and 20 isolates taken from handsand nose of the nurses in charge. From 50 isolate positive S.aureus, PCR results showed single and multiple bandswithin 300 to 900 base pairs (bp) i

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Lueders, Tillmann, and Michael W. Friedrich. "Evaluation of PCR Amplification Bias by Terminal Restriction Fragment Length Polymorphism Analysis of Small-Subunit rRNA and mcrA Genes by Using Defined Template Mixtures of Methanogenic Pure Cultures and Soil DNA Extracts." Applied and Environmental Microbiology 69, no. 1 (2003): 320–26. http://dx.doi.org/10.1128/aem.69.1.320-326.2003.

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ABSTRACT Terminal restriction fragment length polymorphism (T-RFLP) analysis is a widely used method for profiling microbial community structure in different habitats by targeting small-subunit (SSU) rRNA and also functional marker genes. It is not known, however, whether relative gene frequencies of individual community members are adequately represented in post-PCR amplicon frequencies as shown by T-RFLP. In this study, precisely defined artificial template mixtures containing genomic DNA of four different methanogens in various ratios were prepared for subsequent T-RFLP analysis. PCR amplic

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Hubáček, Jaroslav A., H. Pikhart, A. Peasey, R. Kubínová, and M. Bobák. "Nobody Is Perfect: Comparison of the Accuracy of PCR-RFLP and KASP™ Method for Genotyping. ADH1B and FTO Polymorphisms as Examples." Folia Biologica 61, no. 4 (2015): 156–60. http://dx.doi.org/10.14712/fb2015061040156.

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DNA genotyping is among the most common analyses currently performed in scientific research. Two high-throughput genotyping techniques are widely used – the “classic” PCR-RFLP and probe-based methods such as TaqMan® PCR assay or KASP™ genotyping. The probe-based techniques are claimed to be more accurate than PCR-RFLP; however, the evidence for this claim is sparse. We have directly compared results of genotyping of two SNPs (rs1229984 and rs17817449) obtained by the PCR-RFLP and KASP™ in 1,502 adult Caucasians. The results were identical in 97.3 % and 95.9 % cases, respectively. Discrepancies

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Rousseaux, S., M. Olier, J. P. Lemaître, P. Piveteau, and J. Guzzo. "Use of PCR-Restriction Fragment Length Polymorphism of inlA for Rapid Screening of Listeria monocytogenes Strains Deficient in the Ability To Invade Caco-2 Cells." Applied and Environmental Microbiology 70, no. 4 (2004): 2180–85. http://dx.doi.org/10.1128/aem.70.4.2180-2185.2004.

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ABSTRACT A PCR-restriction fragment length polymorphism (RFLP) method was developed in order to screen a large number of strains for impaired adhesion to epithelial cells due to expression of truncated InlA. inlA polymorphism was analyzed by PCR-RFLP in order to correlate inlA PCR-RFLP profiles and production of truncated InlA. Thirty-seven Listeria monocytogenes strains isolated from various sources, including five noninvasive and two invasive reference strains, were screened. Two endonucleases (AluI and Tsp509I) were used, and they generated five composite profiles. Thirteen L. monocytogenes

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Walker, Elaine S., Robert A. Preston, J. Christopher Post, Garth D. Ehrlich, John H. Kalbfleisch, and Karin L. Klingman. "Genetic Diversity among Strains of Moraxella catarrhalis: Analysis Using Multiple DNA Probes and a Single-Locus PCR-Restriction Fragment Length Polymorphism Method." Journal of Clinical Microbiology 36, no. 7 (1998): 1977–83. http://dx.doi.org/10.1128/jcm.36.7.1977-1983.1998.

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Moraxella (Branhamella)catarrhalis, a causative agent of otitis media, sinusitis, and exacerbation of bronchitis, has acquired widespread ability to produce β-lactamase and can be nosocomially transmitted. The typing methods used in epidemiological analyses of M. catarrhalisare not optimal for genetic analyses. Two methods, a multiple-locus Southern blot (SB) method and a single-locus PCR-restriction fragment length polymorphism (RFLP) method, were developed and used to assess genetic diversity and potential clinical and geographic relationships in M. catarrhalis. Nine randomly cloned M. catar

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Tesi sul tema "PCR-RFLP"

Šuranská, Hana. "Identifikace vinných kvasinek metodou PCR-RFLP." Master's thesis, Vysoké učení technické v Brně. Fakulta chemická, 2009. http://www.nusl.cz/ntk/nusl-216494.

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This thesis deals with identification of the wine yeasts by applying the PCR-RFLP method. The identification and characteristic of the yeasts has gone through substantial changes in recent years. There have been introduced new methods of taxonomic classifying based on the molecular methods, which are oriented to easy and fast identification. One of these methods is the PCR-RFLP method. The amplification of the 5•8S-ITS rDNA sequence by the polymerase chain reaction with use of the primers ITS1 and ITS4 leads to the amplification of the specific sequence of DNA. Such multiplied DNA is after rep

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Ziebell, Kim. "Evaluation of PCR and PCR-RFLP protocols to identify the Shiga toxins." Thesis, National Library of Canada = Bibliothèque nationale du Canada, 2000. http://www.collectionscanada.ca/obj/s4/f2/dsk1/tape2/PQDD_0026/MQ51107.pdf.

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Olivová, Radana. "Optimalizace metody PCR-RFLP pro taxonomické zařazení kvasinek." Master's thesis, Vysoké učení technické v Brně. Fakulta chemická, 2009. http://www.nusl.cz/ntk/nusl-216560.

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This thesis deal with optimalization method PCR – RFLP for taxonomy enlistment of yeasts. Conventional identification methods for yeasts are time-consuming. Molecular biological method based on PCR are instrumental towards fast and precise identification as compared to conventional phenotypic methods. In this thesis molecular biological method PCR – RFLP was used for identification and enlistment of yeasts. This metod follow repeating spacers of ribozomal DNA of yeast, characteristic for each species and strain. By the help of PCR were amplified specific partitions of DNA. These fragments of D

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Arraes, Ana Carolina Palmeira. "Detecção da diversidade molecular de Candida spp. isoladas de UTI neonatal." reponame:Repositório Institucional da UFBA, 2013. http://www.repositorio.ufba.br/ri/handle/ri/11817.

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Submitted by Hiolanda Rêgo (hiolandar@gmail.com) on 2013-06-10T20:55:35Z No. of bitstreams: 1 Dissertação_ICS_ Ana Carolina Arraes.pdf: 1876812 bytes, checksum: c4740a70b3675be50d2ebafeb0ba8fe3 (MD5) Made available in DSpace on 2013-06-10T20:55:35Z (GMT). No. of bitstreams: 1 Dissertação_ICS_ Ana Carolina Arraes.pdf: 1876812 bytes, checksum: c4740a70b3675be50d2ebafeb0ba8fe3 (MD5) CAPES A incidência de candidemia tem aumentado nos últimos anos e o espectro das espécies de Candida tem mudado com a emergência das espécies não-albicans e com o aumento da resistência antifúngica. A técn

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Malvárez, Macedo Gabriela. "Aplicação das metodologias PCR/RFLP para caracterização de fungos ectomicorrízicos em eucalipto /." reponame:Repositório Institucional da UFSC, 1999. https://repositorio.ufsc.br/handle/123456789/112290.

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MEDEIROS, Rafael Acioli. "Caracterização da Leishmania infantum e Leishmania (Viannia) braziliensis em cães provenientes da Região Metropolitana do Recife, Pernambuco." Universidade Federal de Pernambuco, 2013. https://repositorio.ufpe.br/handle/123456789/12218.

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Submitted by Milena Dias (milena.dias@ufpe.br) on 2015-03-12T17:45:21Z No. of bitstreams: 2 Dissertação Rafael Medeiros.pdf: 841724 bytes, checksum: 770fac09975639de3352fde1d0ea2ec3 (MD5) license_rdf: 1232 bytes, checksum: 66e71c371cc565284e70f40736c94386 (MD5) Made available in DSpace on 2015-03-12T17:45:21Z (GMT). No. of bitstreams: 2 Dissertação Rafael Medeiros.pdf: 841724 bytes, checksum: 770fac09975639de3352fde1d0ea2ec3 (MD5) license_rdf: 1232 bytes, checksum: 66e71c371cc565284e70f40736c94386 (MD5) Previous issue date: 2013 CAPES As Leishmanioses são uma antropozoonose co

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Björkbom, Tommy. "PCR-RFLP analys av mt-DNA hos Öring (Salmo trutta) i Gävleborgs län." Thesis, University of Gävle, Department of Electronics, Mathematics and Natural Sciences, 2010. http://urn.kb.se/resolve?urn=urn:nbn:se:hig:diva-7624.

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Sultan, Amal Hassan. "The development of molecular tools for identification of Leishmania species by PCR-RFLP." Thesis, University of Liverpool, 2008. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.479067.

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Abreu, Daniel Paiva Barros de. "Caracteriza??o fenot?pica, genot?pica e perfil de sensibilidade a antif?ngicos de isolados cl?nicos de c?es e gatos pertencentes ao Complexo Sporothrix schenckii oriundos do estado do Rio de Janeiro." Universidade Federal Rural do Rio de Janeiro, 2017. https://tede.ufrrj.br/jspui/handle/jspui/2031.

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Submitted by Celso Magalhaes (celsomagalhaes@ufrrj.br) on 2017-09-13T12:31:48Z No. of bitstreams: 1 2017 - Daniel Paiva Barros de Abreu.pdf: 1307092 bytes, checksum: b384d4db2cb316d3f640b111bc8efba4 (MD5) Made available in DSpace on 2017-09-13T12:31:48Z (GMT). No. of bitstreams: 1 2017 - Daniel Paiva Barros de Abreu.pdf: 1307092 bytes, checksum: b384d4db2cb316d3f640b111bc8efba4 (MD5) Previous issue date: 2017-02-20 Conselho Nacional de Desenvolvimento Cient?fico e Tecnol?gico - CNPq Dimorphic fungi belonging to Sporothrix schenckii complex are responsible for sporotrichosis, importa

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Dlapalová, Kristýna. "Izolace a charakterizace autochtonních kvasinek z interspecifické odrůdy vinné révy." Master's thesis, Vysoké učení technické v Brně. Fakulta chemická, 2015. http://www.nusl.cz/ntk/nusl-217112.

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The aim of this thesis is the isolation and identification of yeasts obtained from the wine berries and the characterization of the collection yeast by using processes of PCR - RFLP. The type yeasts were obtained from the collection of yeasts of CCY in Bratislava, yeasts from the wine berries were collected from the species of Hibernal wine from the wineries of Štěpán Maňák. Identification of individual yeast is then based on analysis of the DNA segment in the area of 5,8S - ITS using primers ITS1 and ITS4. The restriction analysis was performed using restriction endonucleases HaeIII, HinfI, H

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Libri sul tema "PCR-RFLP"

A. Zakeri and S.A. Pourbakhsh. Differential diagnosis between ts-11 vaccine strain and field Mycoplasma gallisepticum isolates in clinical samples by PCR-RFLP. Verlag Eugen Ulmer, 2017. http://dx.doi.org/10.1399/eps.2017.195.

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Studies on Genetic Relationships among Six Varieties of Jackfruit in Kerala Employing the Matk Gene Using PCR Technique and Rflp Markers. GRIN Verlag GmbH, 2017.

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Studies on Genetic Relationships among Locally Cultivated Musaceae Varieties in Kerala Employing Rbcl and Matk Gene Using PCR Technique and Rflp Markers. GRIN Verlag GmbH, 2017.

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Studies on Genetic Relationships among Locally Cultivated Citrus Varieties in Kerala Employing Matk and Rbcl Gene Using PCR Technique and Rflp Markers. GRIN Verlag GmbH, 2017.

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Capitoli di libri sul tema "PCR-RFLP"

Frank, J. Howard, J. Howard Frank, Michael C. Thomas, et al. "PCR-RFLP." In Encyclopedia of Entomology. Springer Netherlands, 2008. http://dx.doi.org/10.1007/978-1-4020-6359-6_2812.

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Aguilar, Fernando, and Peter Cerutti. "Genotypic Mutation Assay (RFLP/PCR)." In Technologies for Detection of DNA Damage and Mutations. Springer US, 1996. http://dx.doi.org/10.1007/978-1-4899-0301-3_31.

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Atoui, Ali, and André El Khoury. "PCR-RFLP for Aspergillus Species." In Methods in Molecular Biology. Springer New York, 2016. http://dx.doi.org/10.1007/978-1-4939-6707-0_20.

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Nakashima, Hitoshi, Mitsuteru Akahoshi, and Yosuke Tanaka. "Mutation Detection Using RT-PCR-RFLP." In PCR Protocols. Humana Press, 2003. http://dx.doi.org/10.1007/978-1-4612-0055-0_46.

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Rousseaux, Sandrine, and Michèle Guilloux-Bénatier. "PCR ITS-RFLP for Penicillium Species and Other Genera." In Methods in Molecular Biology. Springer New York, 2016. http://dx.doi.org/10.1007/978-1-4939-6707-0_21.

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Martini, Marta, and Ing-Ming Lee. "PCR and RFLP Analyses Based on the Ribosomal Protein Operon." In Methods in Molecular Biology. Humana Press, 2012. http://dx.doi.org/10.1007/978-1-62703-089-2_15.

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Maramis, Christos, Evangelia Minga, and Anastasios Delopoulos. "An Application for Semi-automatic HPV Typing of PCR-RFLP Images." In Lecture Notes in Computer Science. Springer Berlin Heidelberg, 2010. http://dx.doi.org/10.1007/978-3-642-13775-4_18.

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Duduk, Bojan, Samanta Paltrinieri, Ing-Ming Lee, and Assunta Bertaccini. "Nested PCR and RFLP Analysis Based on the 16S rRNA Gene." In Methods in Molecular Biology. Humana Press, 2012. http://dx.doi.org/10.1007/978-1-62703-089-2_14.

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Scarano, M. T., L. Abbate, S. Ferrante, S. Lucretti, and N. Tusa. "Molecular Characterization of Citrus Symmetric and Asymmetric Somatic Hybrids by Means of ISSR-PCR and PCR-RFLP." In Plant Biotechnology 2002 and Beyond. Springer Netherlands, 2003. http://dx.doi.org/10.1007/978-94-017-2679-5_113.

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Bertaccini, Assunta, Samanta Paltrinieri, and Nicoletta Contaldo. "Standard Detection Protocol: PCR and RFLP Analyses Based on 16S rRNA Gene." In Phytoplasmas. Springer New York, 2018. http://dx.doi.org/10.1007/978-1-4939-8837-2_7.

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Atti di convegni sul tema "PCR-RFLP"

Chuang, Li-Yeh, Yu-Da Lin, Hsueh-Wei Chang, and Cheng-Hong Yang. "An Improved Natural PCR-RFLP Primer Design Method." In Bioengineering (BIBE). IEEE, 2011. http://dx.doi.org/10.1109/bibe.2011.21.

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Amin Al-Rawi, Marwa. "Molecular Differentiation by PCR-RFLP Test of Infectious Laryngotracheitis virus Strains Isolated in Iraq." In III.International Conference on Agricultural and Veterinary Sciences. Rimar Academy, 2025. https://doi.org/10.47832/vet.congress3-6.

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Background: Avian infectious laryngotracheitis (ILT) is a harsh respiratory sickness in chickens. Polymerase chain reaction (PCR) with restriction fragment length polymorphism (RFLP) investigation can be effectively used to differentiate field and vaccine strains. Objective: The aim of this study is to characterize Iraqi isolates by PCR and RFLP to explore the strain's variety. Methodology: This study of infectious laryngotracheitis (ILT) is based on the molecular characterization of samples collected from 12-layer pullet farms in the suburbs of Baghdad - Iraq. A case history was taken for eac

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Maramis, Christos, and Anastasios Delopoulos. "Efficient Quantitative Information Extraction from PCR-RFLP Gel Electrophoresis Images." In 2010 20th International Conference on Pattern Recognition (ICPR). IEEE, 2010. http://dx.doi.org/10.1109/icpr.2010.627.

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Llerena, S. Espezua, and C. D. Maciel. "Exploratory Visualization of RFLP-PCR Genomic Data Using Multidimensional Scaling." In 2008 XXI Brazilian Symposium on Computer Graphics and Image Processing (SIBGRAPI). IEEE, 2008. http://dx.doi.org/10.1109/sibgrapi.2008.42.

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Maramis, Christos F., Anastasios N. Delopoulos, Alexandros F. Lambropoulos, and Sokratis P. Katafigiotis. "A system for automatic HPV typing via PCR-RFLP gel electrophoresis." In 2011 IEEE International Conference on Automation Science and Engineering (CASE 2011). IEEE, 2011. http://dx.doi.org/10.1109/case.2011.6042466.

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Golovenchik, V. I., E. S. Gajduchenko, and T. P. Lipinskaya. "MOLECULAR METHODS FOR IDENTIFYING SPECIES OF THE GENUS TUBENOSE GOBIES: CREATION AND APPLICATION OF PCR-PDF KEYS." In X Международная конференция молодых ученых: биоинформатиков, биотехнологов, биофизиков, вирусологов и молекулярных биологов — 2023. Novosibirsk State University, 2023. http://dx.doi.org/10.25205/978-5-4437-1526-1-309.

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The artical presents data about construction of PCR-PDF keys based on cytb gene to identify species of the genus Tubenose gobies. As a result of the gene analysis, 7 suitable restrictases out of 232 were selected. It is make it possible to identify each species of the genus Tubenose gobies during the restriction analysis. For each restrictase, PCR-RFLP tables and restriction maps were constructed.

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Spiridonova, E. "IDENTIFICATION OF HAPLOTYPE HH6 IN HOLSTINS DAIRY CATTLE POPULATIONS OF DOMESTIC BREEDING." In SCIENTIFIC SUPPORT FOR LIVESTOCK BREEDING IN SIBERIA. Krasnoyarsk Scientific Research Institute of Agriculture is a separate division of the Federal Research Center KSC SB RAS, 2024. https://doi.org/10.52686/conferencearticle_67597cedba9ec9.23164121.

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Modern molecular methods make it possible to conduct DNA diagnostics of breeding animals and identify carriers of hidden genetic defects, thereby excluding them from the breeding process. During the research, a method was developed for performing PCR-RFLP to identify polymorphism of the SDE2 gene.

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Yang, Cheng-Hong, Yu-Huei Cheng, Li-Yeh Chuang, and Hsueh-Wei Chang. "A mismatch PCR-RFLP primer design for SNP genotyping using genetic algorithm." In 2010 9th IEEE International Conference on Cognitive Informatics (ICCI). IEEE, 2010. http://dx.doi.org/10.1109/coginf.2010.5599752.

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Chuang, Li-Yeh, Yu-Da Lin, Hsueh-Wei Chang, and Cheng-Hong Yang. "PCR-RFLP Primer Design Using Particle Swarm Optimization Combined with Chaotic Logistic Map." In 2012 IEEE Workshops of International Conference on Advanced Information Networking and Applications (WAINA). IEEE, 2012. http://dx.doi.org/10.1109/waina.2012.208.

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Maramis, Christos F., Anastasios N. Delopoulos, and Alexandros F. Lambropoulos. "Analysis of PCR-RFLP gel electrophoresis images for accurate and automated HPV typing." In 2010 10th IEEE International Conference on Information Technology and Applications in Biomedicine (ITAB 2010). IEEE, 2010. http://dx.doi.org/10.1109/itab.2010.5687732.

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Rapporti di organizzazioni sul tema "PCR-RFLP"

สิริยะเสถียร, เผด็จ, อุษาวดี ถาวระ та อภิวัฏ ธวัชสิน. การก่อตั้งสายพันธุ์แมลงวันหัวเขียว (Lucilia sericata) เพื่อใช้สำหรับวิธีการรักษาแผลด้วยหนอนแมลงวัน : รายงานการวิจัย (ร่าง). จุฬาลงกรณ์มหาวิทยาลัย, 2009. https://doi.org/10.58837/chula.res.2009.19.

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การสำรวจแมลงวันหัวเขียวใน 6 จังหวัดของประเทศได้แก่ กรุงเทพมหานคร, พิษณุโลก, เชียงใหม่, ตาก, ชุมพร และบุรีรัมย์ การศึกษาทางสัณฐานวิทยาพบเป็นแมลงวันหัวเขียว ชนิด Chrysomya megacephala, Chrysomya rufifacies และ Lucilia cuprina และการศึกษาทางอณูชีววิทยาของยีน cytochrome oxidase ช่วยยืนยันว่าเป็น 3 species นี้ นอกจากนี้การใช้เทคนิค Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP) ยังสามารถช่วยจำแนกแมลงวันหัวเขียวทั้ง 3 ชนิดนี้ได้ PCR ให้ผลผลิต 1324 เบส ในแมลงวันหัวเขียวทั้ง 3 ชนิด การย่อยผลิตภัณฑ์ PCR ด้วย Taqᵅ I and VspI ให้รูปแบบที่จำเพาะในแมลงวันหัวเขียวแต่ละชนิด ประโยชน์ของการใช้เทค

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Michel Jr., Frederick C., Harry A. J. Hoitink, Yitzhak Hadar, and Dror Minz. Microbial Communities Active in Soil-Induced Systemic Plant Disease Resistance. United States Department of Agriculture, 2005. http://dx.doi.org/10.32747/2005.7586476.bard.

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Induced Systemic Resistance (ISR) is a highly variable property that can be induced by compost amendment of potting media and soils. For example, previous studies showed that only 1 of 79 potting mixes prepared with different batches of mature composts produced from several different types of solid wastes were able to suppress the severity of bacterial leaf spot of radish caused by Xanthomonas campestris pv. armoraciae compared with disease on plants produced in a nonamended sphagnum peat mix. In this project, microbial consortia in the rhizosphere of plants grown in ISR-active compost-amended

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Levisohn, Sharon, Mark Jackwood, and Stanley Kleven. New Approaches for Detection of Mycoplasma iowae Infection in Turkeys. United States Department of Agriculture, 1995. http://dx.doi.org/10.32747/1995.7612834.bard.

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Mycoplasma iowae (Mi) is a pathogenic avian mycoplasma which causes mortality in turkey embryos and as such has clinical and economic significance for the turkey breeder industry. Control of Mi infection is severely hampered by lack of adequate diagnostic tests, together with resistance to most antibiotics and resilience to environment. A markedly high degree of intra-species antigenic variation also contributes to difficulties in detection and control of infection. In this project we have designed an innovative gene-based diagnostic test based on specific amplification of the 16S rRNA gene of

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Sittipraneed, Siriporn. การศึกษาการแพร่กระจายของประชากรผึ้งโพรง Apis cerana กลุ่มตอนเหนือและตอนใต้ในบริเวณพื้นที่รอยต่อโดยใช้ดีเอ็นเอเครื่องหมาย : รายงานผลการวิจัย. Chulalongkorn University, 2002. https://doi.org/10.58837/chula.res.2002.30.

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PCR-RFLP of three mtDNA regions ( srRNA gene ,irRNA gene and intergenic COI – COII region) were used to investigated the distribution of northern and southern Apis cerana populations of 89 colonies from Prachuap Khiri Khan and Chumphon provinces. Three, four and eight haplotypes were obtained from DraI digestion of PCR-amplified 410 bp srRNA gene, 755bp IrRNA gene and 1710 bp intergenic COI-COII region,respectively. These three mtDNA regions generated 11 composit haplotypes. Twelve composite haplotypes were generated when samples from Yunnan and Hanoi were included. A UPGMA phenogram based on

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Levisohn, Sharon, Maricarmen Garcia, David Yogev, and Stanley Kleven. Targeted Molecular Typing of Pathogenic Avian Mycoplasmas. United States Department of Agriculture, 2006. http://dx.doi.org/10.32747/2006.7695853.bard.

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Intraspecies identification (DNA "fingerprinting") of pathogenic avian mycoplasmas is a powerful tool for epidemiological studies and monitoring strain identity. However the only widely method available for Mycoplasma gallisepticum (MG) and M. synoviae (MS)wasrandom amplified polymorphic DNA (RAPD). This project aimed to develop alternative and supplementary typing methods that will overcome the major constraints of RAPD, such as the need for isolation of the organism in pure culture and the lack of reproducibility intrinsic in the method. Our strategy focussed on recognition of molecular mark

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นุชประยูร, สุรางค์, จินตนา จิรถาวร, อนุพงค์ สุจริยากุล та อลิสา จันทร์ปี. การศึกษาภูมิคุ้มกันวิทยาเชิงลึกของโรคเท้าช้าง : มุ่งสู่การป้องกันภาวะเท้าช้างและการกำจัดโรคอย่างถาวร : แผนการวิจัย : รายงานการวิจัยฉบับสมบูรณ์. จุฬาลงกรณ์มหาวิทยาลัย, 2009. https://doi.org/10.58837/chula.res.2009.21.

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โรคเท้าช้าง (Lymphatic filariasis) เกิดจากพยาธิ 2 ชนิดหลัก คือ Wuchereria bancrofti และ Brugia malayi ทางองค์การอนามัยโลกได้กำหนดให้โรคเท้าช้างเป็นโรคทางปรสิตที่ควรกำจัดให้หมดไปภายในปี พ.ศ. 2563 โดยมีแนวทางหลักในการควบคุมและป้องกันโรคเท้าช้างคือการจัดให้มีโปรแกรมการรักษาแบบหมู่ โดยให้ยา diethylcarbamazine (DEC) ร่วมกับยา albendazole แก่ประชากรในพื้นที่ที่มีความชุกของโรคสูง และการควบคุมพยาธิภาวะ ปัญหาที่สำคัญของการรักษาโรคเท้าช้าง คือ การใช้ยา DEC ที่ก่อให้เกิดปฏิกิริยาหลังการรักษา กลไกของการเกิดพยาธิสภาพของโรคและการเกิดปฏิกิริยาหลังการรักษายังไม่เป็นที่ทราบแน่ชัดได้ จึงเป็นอุปสรรคที่สำคัญอย่าง

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Sink, Ken, Shamay Izhar, and Abraham Nachmias. Asymmetric Somatic Hybridization: Developing a Gene Transfer System for Solanaceous Vegetable Crops. United States Department of Agriculture, 1996. http://dx.doi.org/10.32747/1996.7613010.bard.

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Highly asymmetric somatic hybrid plants were obtained by PEG/DMSO fusion of gamma irradiated (100, 250, 7500 and 1000 Gy) protoplasts of a (KmR-) interspecific hybrid Lycopersicon esculentum x L. pennellii (EP) with protoplasts of eggplant (E). Somatic hybrid calli were selected based on kanamycin resistance and verified by PCR of the NptII gene, RAPD's and Southern's using potato rDNA pTHG2 probes. Flow cytometry indicated all hybrid calli that did not regenerate shoots were 5-9n. Three asymmetric plants regenerated only from callus close to 4n and such calli oly occurred when EP received 100

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Chaichanawongsaroj, Nuntaree. The role of exercise on serum lipid concentrations varies with Apo E genotype : a study in Thais subjects. Chulalongkorn University, 2004. https://doi.org/10.58837/chula.res.2004.76.

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Apolipoprotein E (apo E) is one of several candidate genes involved in cardiovascular risk. However, the interaction between genes and environmental factors, such as exercise, dietary and lipid lowering intervention, may be affected the incidence of the disease. Our aim was to investigate the effect of physical activity (PA) and apo E polymorphism on plasma lipids and plasma glucose levels in 273 healthy, adult Thais. Plasma lipid and glucose concentrations were determined by enzymatic-colorimetric methods and apo E phenotypes by PCR-RFLP. A PA index was calculated from exercise data which ass

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Gelb, Jr., Jack, Yoram Weisman, Brian Ladman, and Rosie Meir. Identification of Avian Infectious Brochitis Virus Variant Serotypes and Subtypes by PCR Product Cycle Sequencing for the Rational Selection of Effective Vaccines. United States Department of Agriculture, 2003. http://dx.doi.org/10.32747/2003.7586470.bard.

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Objectives 1. Determine the serotypic identities of 40 recent IBV isolates from commercial chickens raised in the USA and Israel. 2. Sequence all IBV field isolates using PCR product cycle sequencing and analyze their S 1 sequence to detennine their homology to other strains in the Genbank and EMBL databases. 3. Select vaccinal strains with the highest S 1 sequence homology to the field isolates and perform challenge of immunity studies in chickens in laboratory trials to detennine level of protection afforded by the vaccines. Background Infectious bronchitis (IB) is a common, economically imp

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Dubcovsky, Jorge, Tzion Fahima, and Ann Blechl. Positional cloning of a gene responsible for high grain protein content in tetraploid wheat. United States Department of Agriculture, 2003. http://dx.doi.org/10.32747/2003.7695875.bard.

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High Grain Protein Content (GPC) is a desirable trait in breadmaking and pasta wheat varieties because of its positive effects on quality and nutritional value. However, selection for GPC is limited by our poor understanding of the genes involved in the accumulation of protein in the grain. The long-term goal of this project is to provide a better understanding of the genes controlling GPC in wheat. The specific objectives of this project were: a) to develop a high-density genetic map of the GPC gene in tetraploid wheat, b) to construct a T. turgidum Bacterial Artificial Chromosome (BAC) libra

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