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Letteratura scientifica selezionata sul tema "Pili (Microbiology)"

Autore: Grafiati

Pubblicato: 4 giugno 2021

Ultima modifica: 20 febbraio 2023

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Articoli di riviste sul tema "Pili (Microbiology)"

1

Hendrickx, Antoni P. A., Claudia M. E. Schapendonk, Miranda van Luit-Asbroek, Marc J. M. Bonten, Willem van Schaik, and Rob J. L. Willems. "Differential PilA pilus assembly by a hospital-acquired and a community-derived Enterococcus faecium isolate." Microbiology 156, no. 9 (September 1, 2010): 2649–59. http://dx.doi.org/10.1099/mic.0.041392-0.

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Abstract (sommario):

Pili are hair-like structures protruding from the cell envelope of bacterial cells. Here, we describe the conditional and differential display of PilA-type pili, and PilE and PilF proteins, encoded from pilin gene cluster 1 at the surface of a hospital-acquired Enterococcus faecium bloodstream isolate (E1165) and a community-derived stool isolate (E1039), at two different temperatures. Both strains have virtually identical pilA gene clusters, as determined by sequencing. Western blotting and transmission immunoelectron microscopy revealed that PilA and PilF assembled into high-molecular-mass p

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2

Sauvonnet, Nathalie, Pierre Gounon, and Anthony P. Pugsley. "PpdD Type IV Pilin of Escherichia coliK-12 Can Be Assembled into Pili in Pseudomonas aeruginosa." Journal of Bacteriology 182, no. 3 (February 1, 2000): 848–54. http://dx.doi.org/10.1128/jb.182.3.848-854.2000.

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ABSTRACT Escherichia coli K-12 possesses at least 16 chromosomal genes related to genes involved in the formation of type IV pili in other gram-negative bacteria. However, E. coli K-12 does not produce type IV pili when grown under standard laboratory conditions. The results of reverse transcription-PCR, operon fusion analysis, and immunoblotting demonstrated that several of the putativeE. coli piliation genes are expressed at very low levels. Increasing the level of expression of the major pilin gene (ppdD) and the linked assembly genes hofB andhofC (homologues of the Pseudomonas aeruginosaty

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3

Graupner, Stefan, Verena Frey, Rozita Hashemi, Michael G. Lorenz, Gudrun Brandes, and Wilfried Wackernagel. "Type IV Pilus Genes pilA andpilC of Pseudomonas stutzeri Are Required for Natural Genetic Transformation, and pilA Can Be Replaced by Corresponding Genes from Nontransformable Species." Journal of Bacteriology 182, no. 8 (April 15, 2000): 2184–90. http://dx.doi.org/10.1128/jb.182.8.2184-2190.2000.

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ABSTRACT Pseudomonas stutzeri lives in terrestrial and aquatic habitats and is capable of natural genetic transformation. After transposon mutagenesis, transformation-deficient mutants were isolated from a P. stutzeri JM300 strain. In one of them a gene which coded for a protein with 75% amino acid sequence identity to PilC of Pseudomonas aeruginosa, an accessory protein for type IV pilus biogenesis, was inactivated. The presence of type IV pili was demonstrated by susceptibility to the type IV pilus-dependent phage PO4, by occurrence of twitching motility, and by electron microscopy. The pilC

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4

Bertrand, Jacob J., Joyce T. West, and Joanne N. Engel. "Genetic Analysis of the Regulation of Type IV Pilus Function by the Chp Chemosensory System of Pseudomonas aeruginosa." Journal of Bacteriology 192, no. 4 (December 11, 2009): 994–1010. http://dx.doi.org/10.1128/jb.01390-09.

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ABSTRACT The virulence of the opportunistic pathogen Pseudomonas aeruginosa involves the coordinate expression of many virulence factors, including type IV pili, which are required for colonization of host tissues and for twitching motility. Type IV pilus function is controlled in part by the Chp chemosensory system, which includes a histidine kinase, ChpA, and two CheY-like response regulators, PilG and PilH. How the Chp components interface with the type IV pilus motor proteins PilB, PilT, and PilU is unknown. We present genetic evidence confirming the role of ChpA, PilG, and PilB in the reg

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5

Villar, Maria T., Jennifer T. Helber, Becky Hood, Michael R. Schaefer, and Rona L. Hirschberg. "Eikenella corrodens Phase Variation Involves a Posttranslational Event in Pilus Formation." Journal of Bacteriology 181, no. 14 (July 15, 1999): 4154–60. http://dx.doi.org/10.1128/jb.181.14.4154-4160.1999.

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ABSTRACT The human pathogen Eikenella corrodens synthesizes type IV pili and exhibits a phase variation involving the irreversible transition from piliated to nonpiliated variants. On solid medium, piliated variants form small (S-phase), corroding colonies whereas nonpiliated variants form large (L-phase), noncorroding colonies. We are studying the molecular basis of this phase variation in the clinical isolate E. corrodens VA1. A genomic fragment encoding the major type IV pilin was cloned from the S-phase variant of strain VA1. Sequence analysis of the fragment revealed four tandemly arrange

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6

Li, Yinuo, Renate Lux, Andrew E. Pelling, James K. Gimzewski, and Wenyuan Shi. "Analysis of type IV pilus and its associated motility in Myxococcus xanthus using an antibody reactive with native pilin and pili." Microbiology 151, no. 2 (February 1, 2005): 353–60. http://dx.doi.org/10.1099/mic.0.27614-0.

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Myxococcus xanthus possesses a social gliding motility that requires type IV pili (TFP). According to the current model, M. xanthus pili attach to an external substrate and retract, pulling the cell body forward along their long axis. By analogy with the situation in other bacteria employing TFP-dependent motility, M. xanthus pili have been assumed to be composed of pilin (PilA) subunits, but this has not previously been confirmed. The first 28 amino acids of the M. xanthus PilA protein share extensive homology with the N-terminal oligomerization domain of pilins in other bacterial species. To

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7

Scheuerpflug, Ina, Thomas Rudel, Roland Ryll, Jasmine Pandit, and Thomas F. Meyer. "Roles of PilC and PilE Proteins in Pilus-Mediated Adherence of Neisseria gonorrhoeae and Neisseria meningitidis to Human Erythrocytes and Endothelial and Epithelial Cells." Infection and Immunity 67, no. 2 (February 1, 1999): 834–43. http://dx.doi.org/10.1128/iai.67.2.834-843.1999.

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ABSTRACT Unlike other type 4 pili, the neisserial pili consist of at least two distinct proteins, the highly variable major subunit PilE forming the pilus fiber and the tip-associated adhesin PilC. PilC protein purified either from gonococci or from Escherichia coliinteracted with different human epithelial cell lines, primary epithelial and endothelial cells. The binding of PilC protein efficiently prevented the attachment of piliated Neisseria gonorrhoeae and Neisseria meningitidis to these cell types. Fluorescent beads coated with pili prepared from piliated wild-type N. gonorrhoeae also ad

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8

Jelsbak, Lotte, and Dale Kaiser. "Regulating Pilin Expression Reveals a Threshold for S Motility in Myxococcus xanthus." Journal of Bacteriology 187, no. 6 (March 15, 2005): 2105–12. http://dx.doi.org/10.1128/jb.187.6.2105-2112.2005.

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ABSTRACT An isopropyl-β-d-thiogalactopyranoside (IPTG)-inducible promoter was constructed in Myxococcus xanthus. The single-copy pilA gene encodes pilin, the monomer unit of M. xanthus type IV pili. To vary the level of pilA expression, we cloned its promoter in front of the lac operator, and a plasmid containing the construct was inserted into the chromosome of a ΔpilA strain. Induction of pilin expression increased smoothly as the dose of IPTG added to the culture was increased. IPTG-induced pilin rescued S motility of the ΔpilA strain to wild-type levels. The rate of S-motile swarming was f

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9

Bakaletz, Lauren O., Beth D. Baker, Joseph A. Jurcisek, Alistair Harrison, Laura A. Novotny, James E. Bookwalter, Rachna Mungur, and Robert S. Munson. "Demonstration of Type IV Pilus Expression and a Twitching Phenotype by Haemophilus influenzae." Infection and Immunity 73, no. 3 (March 2005): 1635–43. http://dx.doi.org/10.1128/iai.73.3.1635-1643.2005.

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ABSTRACT Haemophilus influenzae is considered a nonmotile organism that expresses neither flagella nor type IV pili, although H. influenzae strain Rd possesses a cryptic pilus locus. We demonstrate here that the homologous gene cluster pilABCD in an otitis media isolate of nontypeable H. influenzae strain 86-028NP encodes a surface appendage that is highly similar, structurally and functionally, to the well-characterized subgroup of bacterial pili known as type IV pili. This gene cluster includes a gene (pilA) that likely encodes the major subunit of the heretofore uncharacterized H. influenza

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10

Macdonald, D. L., B. L. Pasloske, and W. Paranchych. "Mutations in the fifth-position glutamate in Pseudomonas aeruginosa pilin affect the transmethylation of the N-terminal phenylalanine." Canadian Journal of Microbiology 39, no. 5 (May 1, 1993): 500–505. http://dx.doi.org/10.1139/m93-071.

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The pili of Pseudomonas aeruginosa are composed of 15-kDa pilin monomers that are synthesized in the cytoplasm and assembled in the membrane. Processing occurs between the synthesis and assembly steps. The propilin is cleaved by a unique leader peptidase encoded by pilD, which is adjacent to the pilin structural gene pilA. This generates an N-terminal phenylalanine that is subsequently methylated by an as yet uncharacterized transmethylase. The pili of P. aeruginosa belong to the type IV class of pilins, which share a highly conserved N-terminal region 35 amino acids in length, containing a sh

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Più fonti

Tesi sul tema "Pili (Microbiology)"

1

Botten, James Alfons Desmond. "Role of sefD and sefR in the biogenesis of Salmonella enterica serovar Enteritidis SEF14 fimbriae." Title page, abstract and contents only, 2001. http://web4.library.adelaide.edu.au/theses/09PH/09phb7512.pdf.

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2

Kennouche, Paul. "New insights into meningococcal pathogenesis : exploring the role of the major pilin PilE in the functions of type IV pili Mechanisms of meningococcal type IV pili multiple functions revealed by deep mutational scanning." Thesis, Sorbonne Paris Cité, 2018. https://wo.app.u-paris.fr/cgi-bin/WebObjects/TheseWeb.woa/wa/show?t=1972&f=12515.

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Les pili de type IV (PT4) sont des filaments micrométriques qui exercent de multiples fonctions à la surface de nombreux procaryotes. Chez Neisseria meningitidis, les PT4 sont des homopolymères de la piline majeure PilE. Leur implication dans l'agrégation interbactérienne et l'adhésion aux cellules humaines les rend centraux dans la virulence du méningocoque. Cependant, les mécanismes permettant aux PT4 d'exercer ces diverses fonctions restent trop élusifs. Durant ce doctorat, nous avons simultanément déterminé les régions de PilE impliquées dans l'assemblage des pili, l'auto-agrégation, l'adh

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3

Paranjpye, Rohinee. "The role of a Vibrio vulnificus type IV pilin in pathogenesis and in persistence in oysters /." Thesis, Connect to this title online; UW restricted, 2005. http://hdl.handle.net/1773/5372.

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4

Choi, Suk Ho. "Binding mechanism of K88ab pili produced by enterotoxigenic Escherichia coli." Diss., Virginia Polytechnic Institute and State University, 1987. http://hdl.handle.net/10919/74764.

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Binding of K88ab pili by brush border membrane and mucus from pig small intestine was characterized by inhibition assay and Western blot. In Western blot, K88ab pili were bound by two major brush border membrane polypeptides with molecular weight of 61,500 and 57,000 in addition to numerous minor polypeptides and a major mucus polypeptide with molecular weight of 27,500. The results from Western blot assays with periodate oxidized and carbamylated brush border membrane and inhibition assay with brush border membrane glycopeptide suggest that amino groups (rather than carbohydrate) present on t

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5

Karlsson, Katarina Flemmer. "Synthesis, conformational analysis, and biological evaluation of peptides from E. coli P pilus proteins." Lund : Organic Chemistry 2, Lund Institute of Technology, Lund University, 1997. http://catalog.hathitrust.org/api/volumes/oclc/39777038.html.

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6

Charles-Orszag, Arthur. "Cellular and molecular mechanisms of human endothelial cell plasma membrane remodeling by Neisseria meningitidis." Thesis, Sorbonne Paris Cité, 2017. http://www.theses.fr/2017USPCB045/document.

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Neisseria meningitidis est une bactérie diderme qui colonise le nasopharynx humain de façon commensale. Occasionnellement, elle franchit la barrière nasopharyngée et accède à la circulation sanguine où elle peut provoquer un choc septique et/ou une méningite Le pouvoir pathogène de N. meningitidis est lié à sa capacité à interagir avec les cellules endothéliales humaines. Après avoir adhéré aux cellules grâce à des organelles filamenteux, les pili de type IV, les bactéries induisent une déformation de la membrane plasmique de la cellule hôte sous la forme de protrusions riches en actine ressem

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7

Kuehn, Joanna Sue Clegg Steven. "Dam methylation and putative fimbriae in Klebsiella pneumoniae." Iowa City : University of Iowa, 2009. http://ir.uiowa.edu/etd/391.

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8

Warren, Matthew J. "Analysis of the role of phosphorylcholine in Neisseria meningitidis /." [St. Lucia, Qld.], 2006. http://www.library.uq.edu.au/pdfserve.php?image=thesisabs/absthe19050.pdf.

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9

Choudhury, Devapriya. "Functional implications of macromolecular recognition : assembly of adhesive pili and enzyme substrate interactions /." Uppsala : Swedish Univ. of Agricultural Sciences (Sveriges lantbruksuniv.), 2001. http://epsilon.slu.se/avh/2001/91-576-5820-X.pdf.

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10

Wright, Denis Sebastian. "Cloning of the Bacteroides nodosus pilin gene and expression in Escherichia coli." Thesis, The University of Sydney, 1985. https://hdl.handle.net/2123/28530.

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The bacterium, Bagteroides nogosu , is the principal causative organism of footrot in sheep. a disease which causes major economic losses in Australia. Current footrot vaccines contain strains from each of the eight serogroups present in Australia. However, they are expensive and difficult to produce because B.nodosus an anaerobe has complex growth requirements and expression of the pilus, a major protective antigen is variable in liquid culture. The pilus (plural, pili) is composed essentially of one subunit protein called pilin and is the antigen which forms the basis for serotyping the org

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Più fonti

Libri sul tema "Pili (Microbiology)"

1

1953-, Jarrell Kenneth F., ed. Pili and flagella: Current research and future trends. Norfolk, UK: Caister Academic Press, 2009.

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2

Holmgren, Anders. Structural studies of PapD, a chaperone protein involved in pili assembly, from E. coli. Uppsala: Sveriges Lantbruksuniversitet, 1993.

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3

Pili and flagella: Current research and future trends. Norfolk, UK: Caister Academic Press, 2009.

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4

1953-, Jarrell Kenneth F., ed. Pili and flagella: Current research and future trends. Norfolk, UK: Caister Academic Press, 2009.

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5

Barocchi, Michele Anne, and John L. Telford. Bacterial Pili: Structure, Synthesis and Role in Disease. CABI, 2014.

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6

Telford, John L., and Michèle Anne Barocchi. Bacterial Pili: Structure, Synthesis and Role in Disease. CABI, 2019.

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7

Per, Klemm, ed. Fimbriae: Adhesion, genetics, biogenesis, and vaccines. Boca Raton: CRC Press, 1994.

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8

Jann, Klaus. Bacterial Adhesins (Current Topics in Microbiology & Immunology, Volume 151). Edited by Klaus Jann. Springer, 1990.

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Capitoli di libri sul tema "Pili (Microbiology)"

1

Schmidt, M. A. "Synthetic Peptides: Prospects for a Pili (Fimbriae)-Based Synthetic Vaccine." In Current Topics in Microbiology and Immunology, 185–204. Berlin, Heidelberg: Springer Berlin Heidelberg, 1990. http://dx.doi.org/10.1007/978-3-642-74703-8_10.

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2

Gelderblom, H., L. Beutin, D. Hadjiyiannis, and H. Reupke. "Rapid Typing of Pili of Pathogenic Escherichia coli by Dispersive Immunoelectron Microscopy." In Rapid Methods and Automation in Microbiology and Immunology, 390–400. Berlin, Heidelberg: Springer Berlin Heidelberg, 1985. http://dx.doi.org/10.1007/978-3-642-69943-6_49.

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3

Ranalli, G., P. Principi, M. Zucchi, F. da Borso, L. Catalano, and C. Sorlini. "Pile Composting of Two-phase Centrifuged Olive Husks: Bioindicators of the Process." In Microbiology of Composting, 165–75. Berlin, Heidelberg: Springer Berlin Heidelberg, 2002. http://dx.doi.org/10.1007/978-3-662-08724-4_14.

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4

Dhakal, B. K., J. M. Bower, and M. A. Mulvey. "Pili, Fimbriae." In Encyclopedia of Microbiology, 470–89. Elsevier, 2009. http://dx.doi.org/10.1016/b978-012373944-5.00051-1.

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5

RCHAPMAN, M., M. AMULVEY, and S. JHULTGREN. "Fimbriae and Pili." In Molecular Medical Microbiology, 177–97. Elsevier, 2002. http://dx.doi.org/10.1016/b978-012677530-3/50228-2.

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6

Volkan, Ender, Vasilios Kalas, and Scott Hultgren. "Pili and Fimbriae of Gram-Negative Bacteria." In Molecular Medical Microbiology, 147–62. Elsevier, 2015. http://dx.doi.org/10.1016/b978-0-12-397169-2.00008-1.

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7

Barnhart, Michelle M., Joel D. Schilling, Fredrik Bäckhed, Agneta Richter Dahlfors, Staffan Normark, and Scott J. Hultgren. "7 Host-pathogen interactions: Structure and function of pili." In Methods in Microbiology, 133–59. Elsevier, 2002. http://dx.doi.org/10.1016/s0580-9517(02)31008-0.

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8

Yan, Kuan, Xianming Zhao, Tong Huang, Wei Li, Yan Ren, and Manzar Abbas. "Microbial Diversity in Sichuan Dark Tea during Pile-Fermentation." In Prime Archives in Microbiology. Vide Leaf, Hyderabad, 2021. http://dx.doi.org/10.37247/pamicr2ed.2.2021.20.

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