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Letteratura scientifica selezionata sul tema "Protoplast fusion"

Autore: Grafiati

Pubblicato: 4 giugno 2021

Ultima modifica: 25 luglio 2025

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Articoli di riviste sul tema "Protoplast fusion"

1

Navrátilová, B. "Protoplast cultures and protoplast fusion focused on Brassicaceae: A review." Horticultural Science 31, No. 4 (2011): 140–57. http://dx.doi.org/10.17221/3809-hortsci.

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The subjects of this article are protoplast isolations and protoplast fusions, in particular their history, a review of factors influencing the protoplasts isolation and fusion, selection of hybrid plants and utilization of somatic hybrids in plant breed-ing. Somatic hybridization through protoplast fusion can overcome sexual incompatibility among plant species or genera; transfer genes of resistance to diseases (viral, bacterial, fungal), pests, herbicides and others stress factors; obtain cybrid plants; transfer cytoplasmic male sterility or incease content of secondary metabolites in hybrid

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2

Perera, Srini C., and Peggy Ozias-Akins. "Regeneration from Sweetpotato Protoplasts and Assessment of Growth Conditions for Flow-sorting of Fusion Mixtures." Journal of the American Society for Horticultural Science 116, no. 5 (1991): 917–22. http://dx.doi.org/10.21273/jashs.116.5.917.

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Petiole protoplasts of the sweetpotato [Ipomoea batatas (L.) Lam.] cultivars Red Jewel and Georgia Jet formed cell walls within 24 hours and divided in 2 to 3 days. Pretreating enzyme solutions with activated charcoal increased the viability and division frequency of protoplasts. Culture of protoplast-donor plants in a medium containing STS did not affect plant growth, protoplasm yield, or viability, but did increase the division frequency. Culture of protoplasts for 24 hours in a medium containing DB, a cell wall synthesis inhibitor, or staining of protoplasts with FDA did not significantly a

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3

Liu, Donglong, and Nancy A. Reichert. "PROTOPLAST ISOLATION AND CULTURE OF KENAF (HIBISCUS CANNABINUS L.)." HortScience 29, no. 7 (1994): 729e—729. http://dx.doi.org/10.21273/hortsci.29.7.729e.

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Protoplast isolation and culture protocols were developed for leaf tissue from 6 kenaf cultivars [Everglades 41 (E41), E71, Guatemala 4 (G4), G45, G51, and Tainung 1]. For protoplast isolation, the best combination of hydrolytic enzymes was cellulysin (1% w/v; Calbiochem) plus macerase (0.5% w/v; Calbiochem), with a 24 hour digestion at 30°C in the dark. Yields reached 7.2 (10)6 protoplasts/g leaf tissue. Protoplast viabilities ranged from 65% to 96%. Minor cultivar differences were observed related to protoplast yield, but all viability estimates were in an acceptable range. Greatest cell div

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4

Ahmed, Mohamed A. A., Miao Miao, Emmanouil D. Pratsinakis, et al. "Protoplast Isolation, Fusion, Culture and Transformation in the Woody Plant Jasminum spp." Agriculture 11, no. 8 (2021): 699. http://dx.doi.org/10.3390/agriculture11080699.

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Plant protoplasts are significant for plant cell culture, somatic cell fusion, genetics, and breeding studies. In addition, in vitro plant regeneration has great importance for developmental biology, manifesting potential applications in agriculture and biotechnology. In this regard, we present a well-established protocol regarding protoplast isolation, cell culture and protoplast fusion of Jasminum spp. In particular, different tissues of Jasminum samab L. and Jasminum mesnyi were employed for protoplast isolation, and stem explants provided a high callus induction rate in a short period of t

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5

Armita, Devi. "Plant Breeding Through Protoplast Fusion." Jurnal Biologi UNAND 8, no. 2 (2020): 42. http://dx.doi.org/10.25077/jbioua.8.2.42-47.2020.

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Protoplast culture (protoplast fusion) is one method of tissue culture that is widely used in plant breeding programs in a relatively short time. This method is used to overcome the problem of plants that are difficult or impossible to cross conventionally as well as used for species improvement by transferring the desired gene from the donor plant to the target plant via protoplast fusion. Protoplast fusion makes it possible to produce plants that are resistant to a disease and various abiotic stresses, rapid growth rates and have a better quantity and quality of metabolites than their parent

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6

Seidel, Thorsten, Philipp Johannes Artmann, Ioannis Gkekas, Franziska Illies, Anna-Lena Baack, and Martina Viefhues. "Microfluidic Single-Cell Study on Arabidopsis thaliana Protoplast Fusion—New Insights on Timescales and Reversibilities." Plants 13, no. 2 (2024): 295. http://dx.doi.org/10.3390/plants13020295.

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Plant cells are omnipotent and breeding of new varieties can be achieved by protoplast fusion. Such fusions can be achieved by treatment with poly(ethylene glycol) or by applying an electric field. Microfluidic devices allow for controlled conditions and targeted manipulation of small batches of cells down to single-cell analysis. To provide controlled conditions for protoplast fusions and achieve high reproducibility, we developed and characterized a microfluidic device to reliably trap some Arabidopsis thaliana protoplasts and induced cell fusion by controlled addition of poly(ethylene glyco

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7

Nolan, Richard A. "Stage-specific changes in cytoplasmic protein synthesis in Entomophaga aulicae protoplasts." Canadian Journal of Microbiology 35, no. 3 (1989): 373–78. http://dx.doi.org/10.1139/m89-057.

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The patterns of protein synthesis associated with three sequential stages in protoplast morphogenesis (spindle-shaped, early fusion sphere, and late fusion sphere protoplasts) of the fungus Entomophaga aulicae were studied using both one-dimensional gels with general protein staining and two-dimensional gels with [35S]methionine protein labelling and fluorography. A total of 332 proteins were observed with 63.5% (211) common to all three developmental stages. Of the individual totals, 3.3% (8 out of 245), 7.3% (22 out of 301), and 4.5% (13 out of 286) of the proteins were unique to the spindle

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8

Chen, Qin, H. Y. Li, Y. Z. Shi, D. Beasley, B. Bizimungu, and M. S. Goettel. "Development of an effective protoplast fusion system for production of new potatoes with disease and insect resistance using Mexican wild potato species as gene pools." Canadian Journal of Plant Science 88, no. 4 (2008): 611–19. http://dx.doi.org/10.4141/cjps07045.

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Somatic hybridization through protoplast fusion is an important alternative approach for overcoming sexual incompatibility between diploid Solanum species and cultivated potatoes. However, compared with other potato species, methods for protoplast isolation and plant generation for several Mexican wild diploid potato species are not well established. In this study, a systematic procedure was designed for the isolation of a large number of high-quality protoplasts from various Mexican wild species that carry high levels of disease (late blight) and insect [Colorado potato beetle (CPB)] resistan

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9

Djajanegara, Ira, and Agus Masduki. "PROTOPLAST FUSION BETWEEN WHITE AND BROWN OYSTER MUSHROOMS." Indonesian Journal of Agricultural Science 11, no. 1 (2013): 16. http://dx.doi.org/10.21082/ijas.v11n1.2010.p16-23.

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Genetic crossing of white oyster mushroom (Pleurotus floridae) to introduce longer storage life trait can only be done within individuals in this particular species. However, longer storage life trait is possessed by brown oyster mushroom (Pleurotus cystidiosus). Therefore, a protoplast fusion experiment between white and brown oyster mushrooms was conducted to obtain an oyster mushroom strain showing high productivity and long storage life. The experiment was done at the biology laboratory of the University of Al Azhar Indonesia from May 2008 to August 2009. Protoplast fusion was done by isol

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10

Nolan, Richard A. "Influence of a negatively charged surface (Teflon disk) on Entomophaga aulicae protoplast morphogenesis under mass fermentation conditions." Canadian Journal of Botany 69, no. 11 (1991): 2578–81. http://dx.doi.org/10.1139/b91-321.

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The effects of a negatively charged surface (Teflon disk) on protoplast morphogenesis for the fungus Entomophaga aulicae under mass fermentation conditions were determined. The control consisted of a vessel lacking such a disk. In the presence of the disk the initial three and sequentially produced protoplast stages (spindle shaped, early fusion sphere, and late fusion sphere protoplasts) recycled with the early fusion sphere predominating. The production of the subsequent and walled stage (i.e., hyphal body) was suppressed. The results are in contrast with those obtained in a previous study u

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Più fonti

Tesi sul tema "Protoplast fusion"

1

Illing, G. T. "Protoplast fusion and regeneration in Streptomyces clavuligerus." Thesis, University of Nottingham, 1987. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.378980.

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2

Mandegaran, Zohreh. "Genetic improvement of roses by protoplast fusion." Thesis, University of East London, 1996. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.339583.

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3

Brites, Anny Stella Monteiro. "Seleção de linhagens de Saccharomyces cerevisiae potencializadas pelo fator Killer, H2S- e o carater floculante." Universidade de São Paulo, 2003. http://www.teses.usp.br/teses/disponiveis/11/11141/tde-19052003-144728/.

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Dentre as características desejáveis em leveduras fermentadoras alcóolicas estão a capacidade de floculação, a não produção de H2S e o caráter "killer". Neste trabalho foram selecionadas sete linhagens de Saccharomyces cerevisiae com algumas destas características, que passaram por testes confirmativos e pela cariotipagem eletroforética resultando na escolha de duas linhagens: ATCC 26602 (altamente floculante) e K1 (H2S - e possuidoras do caráter "killer"). Estas linhagens foram utilizadas em um cruzamento via fusão de protoplasto para se obter um produto de fusão estável com as característica

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4

Maren, Nathan Allen. "Symmetric Protoplast Fusion in Interserial Syringa (Oleaceae) Hybridization." Thesis, North Dakota State University, 2016. https://hdl.handle.net/10365/28026.

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Few other woody plants embody the preeminence of temperate woody plants in garden cultivation like the lilacs. In spite of their relationship, the trees lack the diversity of cultivated floral forms observed within the shrub lineages. Typical selection and cross-pollination schemes within the tree lilacs or between trees and shrubs have failed to yield the diversity of colors and fragrances on a tree form. With somatic fusion in Citrus spp. as a guideline for Syringa spp. protoplast isolation and culture, experiments were designed to optimize the conditions through somatic fusion. Protoplast i

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5

Hansen, Christine S. "Construction of galactose assimilating, carotenoid producing yeasts by protoplast fusion." Thesis, University of British Columbia, 1988. http://hdl.handle.net/2429/27935.

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Protoplasts were prepared from two yeast strains P. rhodozyma (ATCC 24202) and K. fragilis (ATCC 8455). Protoplasts prepared from P. rhodozyma were facilitated by prior growth of the cells in a media containing S-(2-aminoethyl)-L-cysteine. Protoplasts from these two yeast genera were fused either by the use of electrofusion or polyethylene glycol treatment. Stable carotenoid producing cell lines were selected by growth at 30°C on yeast nitrogen base plus galactose. Selected single fusants display taxonomic characteristics common to both genera with a cellular morphology and a carotenoid compo

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6

Raikar, Sanjeev Vencu. "Protoplast fusion of Lolium perenne and Lotus corniculatus for gene introgression." Phd thesis, Lincoln University. Bio-Protection and Ecology Division, 2007. http://theses.lincoln.ac.nz/public/adt-NZLIU20080214.105406/.

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Protoplast fusion of Lolium perenne and Lotus corniculatus for gene introgression by Sanjeev V. Raikar Lolium perenne is one of the most important forage crops globally and in New Zealand. Lotus corniculatus is a dicotyledonous forage that contains valuable traits such as high levels of condensed tannins, increased digestibility, and high nitrogen fixing abilities. However, conventional breeding between these two forage crops is impossible due to their markedly different taxonomic origin. Protoplast fusion (somatic hybridisation) provides an opportunity for gene introgression between these two

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Raikar, S. V. "Protoplast fusion of Lolium perenne and Lotus corniculatus for gene introgression." Diss., Lincoln University, 2007. http://hdl.handle.net/10182/301.

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Lolium perenne is one of the most important forage crops globally and in New Zealand. Lotus corniculatus is a dicotyledonous forage that contains valuable traits such as high levels of condensed tannins, increased digestibility, and high nitrogen fixing abilities. However, conventional breeding between these two forage crops is impossible due to their markedly different taxonomic origin. Protoplast fusion (somatic hybridisation) provides an opportunity for gene introgression between these two species. This thesis describes the somatic hybridisation, the regeneration and the molecular analysis

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8

Johnson, Alexander Arthur Theodore. "Protoplast Fusion for the Production of Intermonoploid Somatic Hybrids in Cultivated Potato." Thesis, Virginia Tech, 1998. http://hdl.handle.net/10919/46514.

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Monoploid potato genotypes represent plant material that is free from the "genetic load" of lethal and severely deleterious alleles normally present in the highly heterozygous cultivated potato species. Field evaluations enabled the identification of agronomically superior monoploid potato genotypes from a population of more than 100 anther-derived monoploids. Chemical fusion and electrofusion between pairs selected from 31 superior monoploids resulted in the production of three different groups of intermonoploid somatic hybrids. The hybridity of somatic hybrid plants and callus

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9

Hothersall, Joanne. "Metabolite production and molecular characterisation of interspecific Aspergilli." Thesis, University of Nottingham, 1998. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.285775.

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10

Gokhale, D. V. "Transfer of DNA coding for cellulases from cellulomonas to bacillus subtilis by protoplast fusion." Thesis(Ph.D.), CSIR-National Chemical Laboratory, Poona, 1988. http://dspace.ncl.res.in:8080/xmlui/handle/20.500.12252/3319.

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Libri sul tema "Protoplast fusion"

1

Mandegaran, Zohreh. Genetic improvement of roses by protoplast fusion. University of East London, 1996.

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2

Clugston, Denise B. Embryo culture and protoplast fusion for the introduction of Mexican wild species germplasm into the cultivated potato. University of Birmingham, 1988.

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3

Wilhelmus Johannes Petrus van Kesteren. Predicting and controlling aggregation and fusion of plant protoplasts. [s.n.], 1994.

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4

Rice, Glenn Edward. Protoplast fusion in the genus Rhodotorula. 1987.

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5

Gleba, Yury Y., and Konstantin M. Sytnik. Protoplast Fusion: Genetic Engineering in Higher Plants. Springer, 2011.

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6

Francisco de Assis Alves Mourão Filho. Protoplast fusion of Citrus for rootstock and scion improvement with emphasis on wide hybridization. 1995.

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Capitoli di libri sul tema "Protoplast fusion"

1

Bengochea, Teresa, and John H. Dodds. "Protoplast fusion." In Plant Protoplasts. Springer Netherlands, 1986. http://dx.doi.org/10.1007/978-94-009-4095-6_4.

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2

Gooch, Jan W. "Protoplast Fusion." In Encyclopedic Dictionary of Polymers. Springer New York, 2011. http://dx.doi.org/10.1007/978-1-4419-6247-8_14602.

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3

Gingold, Elliot B. "Yeast Protoplast Fusion." In Springer Protocols Handbooks. Humana Press, 1986. http://dx.doi.org/10.1007/978-1-60327-405-0_24.

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4

Schieder, O., P. P. Gupta, G. Krumbiegel, and T. Hein. "Protoplast Fusion and Transformation." In Ciba Foundation Symposium 97 - Better Crops for Food. John Wiley & Sons, Ltd., 2008. http://dx.doi.org/10.1002/9780470720783.ch15.

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5

Zimmermann, Martin, and Matthias Sipiczki. "Protoplast Fusion of Yeasts." In Nonconventional Yeasts in Biotechnology. Springer Berlin Heidelberg, 1996. http://dx.doi.org/10.1007/978-3-642-79856-6_2.

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6

Spencer, John F. T., Dorothy M. Spencer, and I. J. Bruce. "Protoplast Formation and Fusion." In Yeast Genetics. Springer Berlin Heidelberg, 1989. http://dx.doi.org/10.1007/978-3-642-73356-7_9.

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Nehls, R., G. Krumbiegel-Schroeren, and H. Binding. "Development of Protoplast Fusion Products." In Results and Problems in Cell Differentiation. Springer Berlin Heidelberg, 1986. http://dx.doi.org/10.1007/978-3-540-39836-3_4.

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Sink, K. C. "Protoplast Fusion and Somatic Hybridization." In Genetics and Breeding of Ornamental Species. Springer Netherlands, 1991. http://dx.doi.org/10.1007/978-94-011-3296-1_4.

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9

Gnanam, Annie Juliet. "Protoplast Fusion Techniques in Fungi." In Laboratory Protocols in Fungal Biology. Springer New York, 2012. http://dx.doi.org/10.1007/978-1-4614-2356-0_45.

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10

Sumeet and K. G. Mukerji. "Protoplast Fusion in Disease Control." In Biotechnological Approaches in Biocontrol of Plant Pathogens. Springer US, 1999. http://dx.doi.org/10.1007/978-1-4615-4745-7_9.

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Atti di convegni sul tema "Protoplast fusion"

1

Kantawang, Titirat, Sompid Samipak, Jumras Limtrakul, and Nattaporn Chattham. "Application of optical tweezers and excimer laser to study protoplast fusion." In International Conference on Photonics Solutions 2015, edited by Surasak Chiangga and Sarun Sumriddetchkajorn. SPIE, 2015. http://dx.doi.org/10.1117/12.2195700.

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2

Sang, Wen-Jiao, Bei-Ping Zhang, Zhen Liu, Lei Wang, Chun-Hua Zhu, and Lan Gao. "Experimental Study on Improving Nitrogen Removal Efficiency of Denitrify Bacteria by Protoplast Fusion." In 2008 2nd International Conference on Bioinformatics and Biomedical Engineering. IEEE, 2008. http://dx.doi.org/10.1109/icbbe.2008.394.

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3

Martasari, Chaireni, Hadi M. Yusuf, Mia Kosmiatin, Ali Husni, and Kurniawan Budiarto. "Ploidy and morphological assessments of protoplast fusion hybrids from Tangerine ‘Madu’ and Mandarin ‘Satsuma’." In INTERNATIONAL CONFERENCE ON ORGANIC AND APPLIED CHEMISTRY (ICOAC) 2022. AIP Publishing, 2024. http://dx.doi.org/10.1063/5.0184800.

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4

Shiwei Wang, Qinghui Wang, Ying Zhai, and Min Wang. "Screening of NHase high-producing Rhodococcus ruber Strain TQD-58 by both parents inactivated protoplast fusion." In 2012 International Symposium on Information Technology in Medicine and Education (ITME 2012). IEEE, 2012. http://dx.doi.org/10.1109/itime.2012.6291408.

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Wijanarka, Wijanarka, Endang Sutariningsih Soetarto, and Kumala Dewi. "Selection of fusan inulinase results of fusion of interspecific protoplas Pichia manshurica and Rhodosporidium paludigenum." In ADVANCES IN INTELLIGENT APPLICATIONS AND INNOVATIVE APPROACH. AIP Publishing, 2023. http://dx.doi.org/10.1063/5.0140276.

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Guosheng Chen, Zegen Wang, Min Wu, Wei Chen, and Min Wang. "Selection of Streptomyces roseosporus strain with high yield by the inactivated parental strain protoplasts fusion." In 2011 International Conference on Remote Sensing, Environment and Transportation Engineering (RSETE). IEEE, 2011. http://dx.doi.org/10.1109/rsete.2011.5965999.

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Rapporti di organizzazioni sul tema "Protoplast fusion"

1

Harman, Gary, and Ilan Chet. Enhancement of Efficacy of Trichoderma spp. for Biological Control Using Protoplast Fusion. United States Department of Agriculture, 1990. http://dx.doi.org/10.32747/1990.7599664.bard.

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2

Sink, Ken, Shamay Izhar, and Abraham Nachmias. Asymmetric Somatic Hybridization: Developing a Gene Transfer System for Solanaceous Vegetable Crops. United States Department of Agriculture, 1996. http://dx.doi.org/10.32747/1996.7613010.bard.

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Highly asymmetric somatic hybrid plants were obtained by PEG/DMSO fusion of gamma irradiated (100, 250, 7500 and 1000 Gy) protoplasts of a (KmR-) interspecific hybrid Lycopersicon esculentum x L. pennellii (EP) with protoplasts of eggplant (E). Somatic hybrid calli were selected based on kanamycin resistance and verified by PCR of the NptII gene, RAPD's and Southern's using potato rDNA pTHG2 probes. Flow cytometry indicated all hybrid calli that did not regenerate shoots were 5-9n. Three asymmetric plants regenerated only from callus close to 4n and such calli oly occurred when EP received 100

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3

Epel, Bernard L., Roger N. Beachy, A. Katz, et al. Isolation and Characterization of Plasmodesmata Components by Association with Tobacco Mosaic Virus Movement Proteins Fused with the Green Fluorescent Protein from Aequorea victoria. United States Department of Agriculture, 1999. http://dx.doi.org/10.32747/1999.7573996.bard.

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The coordination and regulation of growth and development in multicellular organisms is dependent, in part, on the controlled short and long-distance transport of signaling molecule: In plants, symplastic communication is provided by trans-wall co-axial membranous tunnels termed plasmodesmata (Pd). Plant viruses spread cell-to-cell by altering Pd. This movement scenario necessitates a targeting mechanism that delivers the virus to a Pd and a transport mechanism to move the virion or viral nucleic acid through the Pd channel. The identity of host proteins with which MP interacts, the mechanism

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Granot, David, Richard Amasino, and Avner Silber. Mutual effects of hexose phosphorylation enzymes and phosphorous on plant development. United States Department of Agriculture, 2006. http://dx.doi.org/10.32747/2006.7587223.bard.

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Research objectives 1) Analyze the combined effects of hexose phosphorylation and P level in tomato and Arabidopsis plants 2) Analyze the combined effects of hexose phosphorylation and P level in pho1 and pho2 Arabidopsis mutants 3) Clone and analyze the PHO2 gene 4) Select Arabidopsis mutants resistant to high and low P 5) Analyze the Arabidopsis mutants and clone the corresponding genes 6) Survey wild tomato species for growth characteristics at various P levels Background to the topic Hexose phosphorylating enzymes, the first enzymes of sugar metabolism, regulate key processes in plants suc

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