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Tesi sul tema "Randomly Amplified Polymorphic DNA (RAPD)"

Autore: Grafiati

Pubblicato: 4 giugno 2021

Ultima modifica: 4 febbraio 2022

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1

Golembiewski, Robert Craig. "Identification and characterization of creeping bentgrass using randomly amplified polymorphic DNA (RAPD) markers /." The Ohio State University, 1997. http://rave.ohiolink.edu/etdc/view?acc_num=osu1487942739809001.

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2

Woodburn, Mary Alice. "Random amplified polymorphic DNA (RAPD) analysis of Bacillus sphaericus." Thesis, This resource online, 1994. http://scholar.lib.vt.edu/theses/available/etd-07102009-040429/.

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3

Robert, Florence. "Typage de "Candida albicans" par Random Amplified Polymorphic DNA (RAPD)." Paris 5, 1992. http://www.theses.fr/1992PA05P198.

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4

Wei, Ling. "Identification of DNA Markers in Triticum aestivum-Aegilops caudata Additions Lines by Randomly Amplified Polymorphic DNA (RAPD) Technology." DigitalCommons@USU, 1995. https://digitalcommons.usu.edu/etd/4543.

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The objective of this study was to identify DNA markers for each of six added C-genome chromosomes in Triticum aestivum L. cv. 'Alceso'-Aegilops caudata L. addition lines using the randomly amplified polymorphic DNA (RAPD) technique. DNA from Ae. caudata, T. aestivum, amphiploid of T. aestivum X Ae. caudata, and six disomic addition lines of wheat having a pair of Ae. caudata chromosomes was used as the template for the amplification of RAPD markers with a total of 58 random 10-mer oligonucleotide primers. Two primers, OPC-08 and OPJ-16, produced one intense band each from the amphiploid of T.

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5

Papadopoulos, Sarantos. "Untersuchungen genomischer Veränderungen von Mammakarzinomzellen mittels Random Amplified Polymorphic DNA." Doctoral thesis, Humboldt-Universität zu Berlin, Medizinische Fakultät - Universitätsklinikum Charité, 2001. http://dx.doi.org/10.18452/14631.

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Im ersten Teil der Arbeit wurde die random amplified polymorphic DNA (RAPD) Methode für die Anwendung humaner DNA optimiert, indem die Konzentrationen der einzelnen Agentien und das thermische Profil der PCR-Reaktion verändert wurden. Des weiteren wurde der Einfluss von Polymerasen und PCR-Maschinen auf die RAPD, insbesondere auf die Erweiterung des Spektrums der Amplimere und die Reproduzierbarkeit der Reaktionen untersucht. Unsere Untersuchungen haben gezeigt, dass die RAPD eine sehr robuste und reproduzierbare Methode ist. Im zweiten Teil wurden qualitative und quantitative Unterschiede zwi

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6

Campos, Lázara Pereira. "Genome relationships among Lotus species based on random amplified polymorphic DNA (RAPD)." Thesis, McGill University, 1992. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=56888.

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The usefulness of RAPDs (Random Amplified Polymorphic DNA) to distinguish among different taxa of Lotus was evaluated. The following species were included: L. corniculatus, L. tenuis, L. alpinus, L. japonicus, and L. uliginosus. Several accessions for each species were studied. Following DNA extraction, amplification reactions were performed in a Hybaid DNA Thermal Cycler, and the product visualized according to a standard procedure. Twenty primers were used for each species/accession. Clear bands and several polymorphisms were obtained for all primers. A phenogram was drawn based on the genet

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7

Rinaldi, Catherine. "Authentication of the Panax genus plants used in Traditional Chinese Medicine (TCM) using Randomly Amplified Polymorphic DNA (RAPD) analysis." University of Western Australia. Centre for Forensic Science, 2007. http://theses.library.uwa.edu.au/adt-WU2008.0054.

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[Truncated abstract] Traditional medicines are used by millions of people throughout the world as their primary source of medical care. A range of materials are in used traditional medicines including plant and animal parts. Even though the traditional medicine trade is estimated to be worth sixty billion dollars annually the trade remains largely unregulated. Unscrupulous practices by vendors to increase their profit margins such as substituting and adulterating expensive material with cheaper varieties go unchecked. This can be dangerous to consumers because some substitutions involve poison

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8

Patel, Sushma M. "Ribosomal RNA genes and RAPD for Cryptosporidium species and subspecies discrimination." Thesis, Coventry University, 1997. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.389769.

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9

Hughes, Greta. "Population structure and genetics of the European lobster Homarus gammarus." Thesis, Bangor University, 2000. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.341219.

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10

Góis, Plácia Barreto Prata. "Aplicação do RAPD (Randomly Amplified Polymorphic dna) para tipagem molecular de amostras de Salmonella isoladas de diversas fontes da cidade de Aracaju-SE." Universidade Federal de Sergipe, 2006. https://ri.ufs.br/handle/riufs/3673.

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Coordenação de Aperfeiçoamento de Pessoal de Nível Superior<br>The Salmonella infection is a relevant problem in the public health, being one of the most important causes of the world´s enteric pathologies, with 1,3 million ill people, resulting in 600 deaths/year. The Salmonella spp. transmission occurs especially through water consumption and contaminated food. The diagnosis is realized using biochemical, serologic, and molecular tests. The RAPD (Randomly Amplified Polymorphic DNA) test is able to detect the genetic variation present in the isolated Salmonella spp. samples, allowing a mole

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11

Allnutt, Theodore Richard. "The study of genetic variation in trees using the random amplified polymorphic DNA (RAPD) technique." Thesis, Liverpool John Moores University, 1996. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.319848.

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12

Atienzar, Franck Andre. "Development of the random amplified polymorphic DNA (RAPD) technique to measure the effects of genotoxinsin aquatic organisms." Thesis, University of Plymouth, 2000. http://hdl.handle.net/10026.1/1757.

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Studies were undertaken to evaluate the potential of random amplified polymorphic DNA (RAPD) to detect DNA effects (including DNA damage and mutations) in aquatic invertebrates, following their exposure to a variety of environmental contaminants under laboratory conditions. After rigorous optimisation of the RAPD method, the protocol, which used a high annealing temperature (50"C for 10-mer primers), was found to generate good-quality DNA profiles from groups of organisms belonging to the bacterial, plant and animal kingdoms. The RAPD method was initially used to detect benzo(a)pyrene [B(

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13

Dias, Paula Menna Barreto. "Caracterização de espécies brasileiras de Adesmia DC. por RAPD." reponame:Biblioteca Digital de Teses e Dissertações da UFRGS, 2003. http://hdl.handle.net/10183/4379.

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Dentro do gênero Adesmia, as técnicas moleculares ainda não foram empregadas na caracterização de germoplasma e na análise da diversidade genética das espécies brasileiras que compôem o gênero. Portanto os objetivos deste trabalho foram: caracterizar, com a utilização de marcador molecular do tipo RAPD, as espécies brasileiras do gênero Adesmia DC; com base nestas informações estabelecer relações de diversidade genética entre as espécies e os acessos analisados; relacionar dados de diversidade com dados morfológicos e de reprodução.

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14

Kongkiatngam, Prasert. "Genetic studies of red clover (Trifolium pratense L.) using morphological, isozyme and random amplified polymorphic DNA (RAPD) markers." Thesis, McGill University, 1995. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=29066.

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Genetic variation within and between two cultivars of red clover (Trifolium pratense L.), Essi from Europe and Ottawa from Canada was estimated using morphological, isozyme and random amplified polymorphic DNA (RAPD) markers. A total of 21 enzyme-coding loci with 43 alleles was detected using twelve enzyme systems. The mean number of alleles per locus was 1.81 in Essi and 1.67 in Ottawa. Nine 10-mer primers were used to assay 20 individuals from each cultivar for RAPD markers. Each primer gave from 7 to 20 amplified bands with an average of 14.8 bands per primer. High within-cultivar variation

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15

Bardakci, F. "Applications of the random amplified polymorphic DNA (RAPD) technique in tilapia (Pisces cichlidae) : species, subspecies and sex identification." Thesis, Swansea University, 1995. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.636032.

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The RAPD reaction was optimised and factors affecting RAPD results were examined to obtain reproducible and reliable DNA markers for the tilapia genome. Homogeneity between RAPD samples during the preparation, amplification and electrophoresis is essential for obtaining reproducible RAPD fingerprint patterns. RAPD markers were used to discriminate between several commercially important species and subspecies of tilapia. The technique was efficient in revealing genetic variability both within and between populations. Phylogenetic relationships between these species and subspecies showed broad a

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16

Alamri, Sarah. "COMPARATIVE ANALYSIS OF SOYBEAN (GLYCINE MAX) ACCESSIONS USING INTER SIMPLE SEQUENCE REPEAT (ISSR) AND RANDOM AMPLIFIED POLYMORPHIC DNA (RAPD) MARKERS." Thesis, Laurentian University of Sudbury, 2014. https://zone.biblio.laurentian.ca/dspace/handle/10219/2201.

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Soybean (Glycine max) is an important crop in the world in terms of total production and usage. It is also among the least diverse species. The main objectives of the present study were 1) to determine differences between ISSR and RAPD marker systems in detecting genetic variation in soybeans and 2) to identify and characterize accession- diagnostic molecular markers in G. max accessions. Genomic DNAs from 108 G. max accessions from 11 different gene pools were analyzed using several ISSR and RAPD primers. The levels of polymorphic loci detected with the two marker systems were in general mode

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17

Monteiro, Alexandre Amorim. "Padronização de metodologia para caracterização molecular por RAPD ? Random Amplified Polymorphic DNA, de bactérias ácido láticas isoladas de leite cru." Universidade Estadual de Londrina. Centro de Ciências Agrárias. Programa de Pós-Graduação em Ciência Animal, 2008. http://www.bibliotecadigital.uel.br/document/?code=vtls000129296.

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No Brasil, de modo geral, o leite é obtido sob condições higiênico-sanitárias deficientes e, em conseqüência, apresenta baixa qualidade microbiológica, constituindo um risco à saúde da população quando consumido sem tratamento térmico. Desta forma, vários grupos de microrganismos indesejáveis podem ser detectados no leite cru, gerando alterações nas mais variadas condições. Em resposta a esta situação, diferentes estratégias estão em estudo para melhoraria da qualidade. Uma dessas estratégias é a bioconservação, que vem sendo cada vez mais estudada, devido seu enorme potencial de aplicação nos

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18

CORREIA, Janaina. "Clonagem e sequenciamento de um fragmento de DNA específico de um isolado virulento de Paracoccidioides brasiliensis." Universidade Federal de Pernambuco, 2004. https://repositorio.ufpe.br/handle/123456789/706.

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Made available in DSpace on 2014-06-12T15:04:52Z (GMT). No. of bitstreams: 2 arquivo4510_1.pdf: 1312401 bytes, checksum: 103b70bf03d8851fef2766e8bd0290f9 (MD5) license.txt: 1748 bytes, checksum: 8a4605be74aa9ea9d79846c1fba20a33 (MD5) Previous issue date: 2004<br>Paracoccidioides brasiliensis é um fungo dimórfico e agente etiológico da paracoccidioidomicose, uma micose sistêmica de evolução aguda ou crônica que se não diagnosticada e tratada a tempo pode ser fatal. Um método molecular para caracterização e detecção de P. brasiliensis foi desenvolvido a partir da clonagem e do sequencia

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19

Ivgin, Tunca Rahsan. "Determination And Comparison Of Genetic Variation In Honey Bee (apis Mellifera L.)populations Of Turkey By Random Amplified Polymorphic Dna And Microsatellite Analyses." Phd thesis, METU, 2009. http://etd.lib.metu.edu.tr/upload/3/12610443/index.pdf.

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We analyzed a total of 760 worker bees, two samples per colony, 390 colonies in 26 provinces in Turkey to determine and compare the genetic variation of Turkish honey bee (Apis mellifera L.) populations using 10 primers for RAPD and 6 microsatellite loci. Mean gene diversity levels ranged from 0.035 (Sanliurfa) to 0.175 (Antalya) for RAPD and 0.449 (Mugla) to 0.739 (Artvin) for microsatellite markers. Private band patterns and alleles, pairwise FST values support that the Anatolian honey bees belong to C lineage except for Hatay and Sanliurfa populations illustrated from previous findings of m

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20

Sovic, Michael G. "An Evaluation of Fluorescent Randomly Amplified Polymorphic DNA (FRAPD) as a Tool for Identifying Species Hybrids, and the Application of these Markers to Questions of Hybridization in Two Groups of Ohio River Basin Fishes." The Ohio State University, 2011. http://rave.ohiolink.edu/etdc/view?acc_num=osu1308056342.

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21

Sarre, Caroline. "Utilisation de la méthode Random Amplified Polymorphic DNA (RAPD) pour le typage moléculaire du genre Scedosporium : application à des isolats collectés chez des patients atteints de mucoviscidose." Paris 5, 1999. http://www.theses.fr/1999PA05P039.

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22

Barcelos, Ivanildes Solange da Costa. "Polimorfismo por Random Amplified Polymorphic DNA (RAPD) em metacestódeos de Taenia solium provenientes de diferentes áreas geográficas do Brasil e a reatividade de anticorpos IgG séricos de pacientes com neurocisticercose frente aos isolados obtidos." Universidade Federal de Uberlândia, 2006. https://repositorio.ufu.br/handle/123456789/16559.

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Neurocysticercosis (NC) is a polymorphic disease and the immune response in human carrier is heterogenic. In this study, 35 primers were used for amplifications by Random Amplified Polymorphic DNA (RAPD) of Taenia solium metacestodes, from five different geographic areas in Brazil: 1) Distrito Federal (DF), Center West; 2) Barreiras (BA), Northeast and Southeast; 3) Hydro Basin of the Mosquito River (North of Minas Gerais, RM-MG), 4) São Paulo (SP) and 5) Uberaba (Minas Gerais, UB-MG). Metacestodes saline crude extracts of four populations (DF, BA, RM-MG e SP) were used for the detection of sp

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23

Beeson, Keri Elizabeth. "Differentiation of plasmids in marine diazotroph assemblages determined by randomly amplified polymorphic DNA analysis." Thesis, Georgia Institute of Technology, 2001. http://hdl.handle.net/1853/25366.

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Corniquel, Béatrice. "Construction de banques d'ADNc de palmier dattier (Phoenix dactylifera L. ). Différenciation de cultivars par RFLP à l'aide des ADNc : isolement et caractérisation d'un ADNc polymorphe. Isolement et caractérisation d'un ADNc codant pour la glutamine synthetase cytosolique." Angers, 1994. http://www.theses.fr/1994ANGE0014.

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La caractérisation des cultivars de palmier dattier a été réalisée par des analyses RFLP et RAPD à partir des feuilles internes des rejets. Des sondes ont été isolées d'une banque d’ADNc construite à partir d'ARNM extraits de cals organogènes du cultivar bou sthammi noire. La sonde ADNc 1 a permis d'obtenir neuf profils d'hybridation distincts pour chacun des neuf cultivars étudiés. Des profils d'amplification distincts pour trois cultivars ont été obtenus par RAPD avec des amorces oligonucléotidiques. Des analyses RFLP ont été aussi réalisées sur du matériel maintenu in vitro ou issu de cultu

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MARTINS, Walter Fabrício Silva. "Diversidade genética de populações naturais de Anthonomus grandis Boheman (Coleoptera : Curculionidae)." Universidade Federal de Pernambuco, 2006. https://repositorio.ufpe.br/handle/123456789/716.

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Made available in DSpace on 2014-06-12T15:04:58Z (GMT). No. of bitstreams: 2 arquivo1766_1.pdf: 916614 bytes, checksum: d16125fdbdb2b99a4f6d33952c69a16b (MD5) license.txt: 1748 bytes, checksum: 8a4605be74aa9ea9d79846c1fba20a33 (MD5) Previous issue date: 2006<br>O bicudo do algodoeiro, Anthonomus grandis Boheman, é considerado a maior praga da cotonicultura mundial, ocasionando danos que repercutem principalmente na produtividade, qualidade do algodão colhido e gasto com medidas de controle. Neste estudo foi realizada pela primeira vez, uma análise da diversidade e estrutura genética da

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26

Wang, Jau-Yueh, and 王昭月. "Identifications of Capsicum spp. by Isozymes and Randomly Amplified Polymorphic DNA (RAPD) Markers." Thesis, 1995. http://ndltd.ncl.edu.tw/handle/94172284351782431844.

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碩士<br>國立中興大學<br>園藝學系<br>83<br>Morphological traits,isozymes and randomly amplified polymor- phic DNA (RAPD) markers were used to study the identification of Capsicum species.The purposes of this study were to establish molecular markers for classification of the Capsicum spp., to establish the genetic linkage relationships and a quick, technique for the identification of seed purity of Capsicum spp. The results of isozyme analysis indicated that there were significantly different in the zy

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Wang, Zhao-Yue, and 王昭月. "Identifications of capsicum spp. by isozymes and randomly amplified polymorphic DNA (RAPD) markers." Thesis, 1995. http://ndltd.ncl.edu.tw/handle/25557591573302930114.

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Wei, Jun-hung, and 魏俊宏. "Cloning Sex Relatine Genes of Carica papaya L. by Randomly Amplified Polymorphic DNA (RAPD)." Thesis, 1998. http://ndltd.ncl.edu.tw/handle/84072335020026873496.

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碩士<br>東吳大學<br>微生物學系<br>86<br>Carica papaya L. is a trimonoecious (polygamous) species that individualplants can be classified to three basic sex types:hermaphrodite, pistillate(female) and staminate (male).The results of cross- and self-pollinations in papaya have showed that the determination of sex type was caused by inherited factors.In this work, we try to clone the different DNA fragments between staminate and pistillate by randomly amplified polymorphic DNA (RAPD).In order to isolate the sex determination genes of papaya, we have screenedwith 200 primers and have cloned two specific DNA

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Liu, Yi-Chin, and 劉怡君. "Identification of Cynodon dactylon (L.) Pers. by Morphological Traits and Randomly Amplified Polymorphic DNA (RAPD) Markers." Thesis, 1996. http://ndltd.ncl.edu.tw/handle/67411824389673206103.

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碩士<br>國立中興大學<br>園藝學系<br>84<br>Summary The clusis analysis with both the morphological traits and the RAPD marker data were used to identify 16 bermudagrass cultivars. It was the first time RAPD method been used on bermudagrass for cultivar identification. The goals for this experiment are: 1)To build up a database for bermudagrass morphological characters, and use the data for cluster analysis to construct relationship data between cultivars. 2)Testing the reliability of RAPD test compa

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Blue, Gillian Margaret. "Assessment of genetic diversity and DNA fingerprinting of the Cape parrot (Poicephalus robustus) using randomly amplified polymorphic DNA (RAPD)." Thesis, 2004. http://hdl.handle.net/10413/10126.

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The Cape parrot (Poicephalus robustus) is South Africa's only endemic parrot. It has become increasingly rare in recent years, with fewer than 500 birds left in the wild, and is now regarded as endangered. Possible factors contributing to this rapid decline in numbers include habitat loss, food shortage, disease and illegal trafficking and trading in the species. Habitat loss and food shortage have been brought about by the rapid destruction of the yellowwood trees in the afromontane forests in South Africa and have played a role in reducing the population numbers. The Psittacine beak and feat

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Nagaraja, G. M. "Genetic analysis of Bombyx mori using Random amplified Polymorphic DNA (RAPD) markers." Thesis, 1999. http://hdl.handle.net/2009/1354.

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Liu, Chi-An, and 劉啟安. "Application of Random Amplified Polymorphic DNA (RAPD) for Species-Specific Identification in Parrots." Thesis, 2016. http://ndltd.ncl.edu.tw/handle/42374419271822770641.

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碩士<br>國立中興大學<br>動物科學系所<br>104<br>In taxonomy, parrots belong to gegnus Psittaciformes and distribute mainly between north and sough latitudes. Until now, it is known that there are about 374 kinds of parrots around the world. Although Taiwan is not the original habitat of parrots, parrots are one of the most popular companion animals in Taiwan due to their beautiful plumage and high intelligence. However, all of the parrot species, except for Agapornis roseicollis, Melopsittacus undulates, Nymphicus holandicus and Psittacula krameri, are endangered and protected by CITES (Convention on Interna

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Edwards, Nicola Rachel. "Optimisation of the randomly amplified polymorphic DNA (RAPD) technique for the characterisation of selected South African maize (Zea mays L.) breeding material." Thesis, 2000. http://hdl.handle.net/10413/9812.

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Maize (Zea mays L.) is an important agronomic crop with the maize industry forming an important component of the South African economy. Considerable effort has been directed towards the genetic improvement of maize through both conventional breeding and biotechnology. Genotype identification by DNA fingerprinting is becoming an important activity in plant breeding. A widely used molecular based and relatively inexpensive method for DNA fingerprinting is the randomly amplified polymorphic DNA (RAPD) technique. The RAPD technique was tested in this study for its potential use in maize breeding p

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Mailer, Rodney J. "Cultivar identification of oilseed rape, Brassica napus, by high performance liquid chromatography (HPLC) and randomly amplified polymorphic DNA (RAPDs)." 1993. http://hdl.handle.net/1993/17765.

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Wu, Hui-Ju, and 吳慧如. "Availability of Using RAPD(Random Amplified Polymorphic (DNA) Segments as Hybridization Probes in Sorghum RFLP analysis." Thesis, 1993. http://ndltd.ncl.edu.tw/handle/96177597261715815246.

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碩士<br>國立臺灣大學<br>農藝學系<br>81<br>The molecular markers had been developed as a powerful tool for the studies in plant genetics and breeding. There are two types of molecular markers : one is restriction fragment length polymorphism (RFLP) marker, and the other is random amplified polymorphic DNA (RAPD) marker. In order to obtain the RFLP markers, it should take a long time and spend much resources to construct and screen a genetic library. However, since the polymerase chain reaction (PCR)

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Naguran, Riann. "Fingerprinting of full and half-sib black wattle (Acacia mearnsii) progenies using Random Amplified Polymorphic DNA (RAPD)." Thesis, 2005. http://hdl.handle.net/10413/4745.

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Black wattle (Acacia mearnsii), which belongs to the genus Acacia, is one of the many species of trees or hardwoods grown commercially in South Africa. Black wattle is a species indigenous to Australia and was introduced into South Africa by the van der Plank brothers in 1864. These trees are grown in South Africa because of its tannin-rich bark, the extract of which is used by the leather tanning industry. Black wattle is also grown for its timber, timber products and pulp. The introduction and cultivation history of black wattle suggests that the South African plantations contain limited gen

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Lee, Shao-Pei, and 李紹珮. "Analysis of random amplified polymorphic DNA (RAPD) markers associated with yield traits of tomato under heat stress." Thesis, 2004. http://ndltd.ncl.edu.tw/handle/82955925450323541644.

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碩士<br>中國文化大學<br>生物科技研究所<br>92<br>Tomato contains many kinds of chemical constituents, such as Vitamin C and lycopene, which are considered as free radicals scavenger and thus protects people from some chronic disorders or reduces the rate of some cancers. Most tomato varieties are adaptive to warm and dry weather, and don’t show heat tolerance. The optimum temperature for growing tomato is 15-20/20-26 °C night/ day. During summer time in Taiwan, the production of tomato is decreased due to high temperature and high rainfall. Heat tolerance of tomato is a quantitatively inherited t

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Hou, ya-wen, and 侯雅文. "Construction of a linkage map based on RAPD (Random Amplified Polymorphic DNA)markers in Cucumis melo L." Thesis, 1996. http://ndltd.ncl.edu.tw/handle/71442632451340748353.

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碩士<br>輔仁大學<br>生物學系<br>84<br>This research project is intended to construct a RAPD (random amplified polymorphic DNAs) linkage map of Cucumis melo L. Conditions such as the amount of DNA template,the concentration of Mg+2 ions available,the annealing temperature of the primer used and its duration were optimized to generate reproducible RAPDpatterns.Furthermore,a F2 population was established from a F1 plant obtained from a cross of a Eastern melon ♀ parent (cv. SLK-V-052) and a musk melon ♂

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39

Lee, Huey Jen, and 李慧真. "Analysis of Genetic Variation of Pseudocercospora fuligena and P. atromarginalis by Random Amplified polymorphic DNA (RAPD) technique." Thesis, 1996. http://ndltd.ncl.edu.tw/handle/31131496415158966156.

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碩士<br>國立中興大學<br>植物病理學系<br>84<br>The fungi tested in this study included each 33 isolates of Pseudocercosporafuligena (on Lycopersicon esculentum ) and P. atromarginalis (on Solanumnigrum), and 20 other isolates of Cercospora spp. and Pseudocercospora spp. wereused to perform analysis of their genetic variation by RAPD. Based on thepreliminary tests of searching suitable RAPD conditions, the results revealedthat optimal reaction mixtures contained DNA ( 50 ng ), 1 x buffe

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40

Lin, Cheng Chieh, and 林政潔. "Application of Morphological Traits and Random Amplified Polymorphic DNA (RAPD) Markers on Cultivar Identification of Capsicum annuum L." Thesis, 1998. http://ndltd.ncl.edu.tw/handle/75770954719698825603.

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碩士<br>國立中興大學<br>園藝學系<br>86<br>Morphological traits and randomly amplified polymorphic DNA (RAPD) markers are used to identify their relationship of 49 pepper(Capsicum annuum L.) cultivars from Know You and Evergrow Seed Companies. Twenty five horticultural traits based on the classification system of National Plant Genetic Resource Center showed that the morphological traits of seed, leaf, flower, and fruit can be used to distinguish 49 Capsicum annuum L. cultivars. By utilization of the di

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41

Shing, Jong，Ming, and 鍾明勳. "Use Random Amplified Polymorphic DNA (RAPD) Analysis on the Genetic Relationship among Phyllostachys Species and among Bambusa Species." Thesis, 1996. http://ndltd.ncl.edu.tw/handle/62824528169573568914.

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42

Tsai, Tzung-Yu, and 蔡宗育. "Genetic Diversity in butterhead and crisphead lettuce (Lactuca sativa L.) by using Morphological Traits, Random Amplified Polymorphic DNA (RAPD) and Sequence Characterized Amplified Regions (SCAR) Markers." Thesis, 2008. http://ndltd.ncl.edu.tw/handle/59226251002783909910.

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碩士<br>國立中興大學<br>園藝學系所<br>96<br>The genetic diversity of 31 butterhead (BUT) and 26 crisphead (CRP) accessions were evaluated by morphological traits, RAPD (Random Amplified Polymorphic DNA) and SCAR (Sequence Characterized Amplified Regions). Data for 19 morphological traits were subjected to a genetic diversity analysis, after which a UPGMA cluster analysis was performed. The 57 accessions were clustered into 2 groups according to type with genetic similarity of 0.38. The first group included 15 accessions form Tainan and 3 accessions form the National Germplasm of the USA, which were cluster

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43

Min, Tee See, and 鄭思敏. "Genetic Diversity in Colocasia and Xanthosoma Based on Morphological Traits, Random Amplified Polymorphic DNA (RAPD) and Simple Sequence Repeats (SSR) Markers." Thesis, 2005. http://ndltd.ncl.edu.tw/handle/28240061297483500453.

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碩士<br>國立中興大學<br>園藝學系<br>93<br>Abstract The genetic diversity of 30 Colocasia (KCC) and 14 Xanthosoma (KCX) accessions collected from Taiwan and other places were evaluated by morphological traits, RAPD (randomly amplified polymorphic DNA) and SSR (simple sequence repeats). Data for 28 characters of shoot, 9 characters of corm and 2 indexes were subjected to a genetic diversity analysis and the UPGMA cluster analysis was performed. The 44 accessions were clustered into 2 groups according to genus with genetic similarity of 0.32. The Colocasia accessions were clustered into 3 subgroup

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Shen, Zi Ru, and 沈姿如. "Stduy on the taxonomic identity of schima superba var. superba and S. superba var. kankaoensis by random amplified polymorphic DNA (RAPD)." Thesis, 1996. http://ndltd.ncl.edu.tw/handle/27494675779896588354.

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Abstract (sommario):

碩士<br>國立中興大學<br>森林學研究所<br>84<br>Schima superba var. superba is an important native and plantation species in Taiwan and S. superba var. kankaoensis is a variety of S. superba var. superba. Since there is no reli-able morphological characteristic to distinguish the two of them, we tried to use a new technique, random amplified polymorphic DNA(RAPD), to study the taxonomic identity of the two species. Twenty arbitrary sequenced primers(10 nucleotides in length) were selected to proceed RAPD-PCR. One hundred and forty seven bands were recorded and 90(%) of them were polymorphic bands. DNA markers

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45

Sewpersad, Yaksha. "Estimation of genetic variation and marker identification in black wattle (Acacia mearnsii De Wild) with RAPD fingerprinting." Thesis, 2004. http://hdl.handle.net/10413/10016.

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46

Chiou, Chin-Chun, and 邱金春. "Identification of Cymbidium spp. by randomly amplified polymorphic DNA markers." Thesis, 1997. http://ndltd.ncl.edu.tw/handle/00190219285461565041.

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Abstract (sommario):

碩士<br>國立中興大學<br>園藝學系<br>85<br>Morphological traits and randomly amplified polymorphic DNA (RAPD) markerswere used to study the identification and discrimination of Cymbidium species.The purposes of this study were to establish the molecular markers for discrimination of the Cymbidium spp. and cultivars and to explore the relationships of genetic linkage among the Cymbidium spp. and cultivars. The data of phenotype characters and growth habit showed that the tenCymbidium spp. can be discr

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47

Lin, Yin-Shan, and 林吟珊. "Identification of Peanut Genetic diversity by Randomly Amplified Polymorphic DNA markers." Thesis, 2001. http://ndltd.ncl.edu.tw/handle/77913625353644316682.

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碩士<br>國立高雄師範大學<br>生物科學研究所<br>89<br>Abstract The 115 peanut varieties as materials in this research include important released breeding varieties and common used progenitors of breeding in the past in Taiwan, introduced varieties with rust and leafspot resistance, and the breeding varieties recently released from Tainan District Agricultural Improvement Station. Observing the phenotypes, characters of pods and seeds of these 115 varieties, varieties in each group have above 90% relativity in four traits, including procumbent, decumbent or erect growth habits, early or late

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48

Lee, Shough-Peng, and 李碩朋. "Identification of native Dendrobium species by Randomly Amplified Polymorphic DNA Markers." Thesis, 2002. http://ndltd.ncl.edu.tw/handle/35571663803232979822.

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碩士<br>國立中興大學<br>園藝學系<br>90<br>Genetic diversity and relatedness were assessed among 19 Dendrobium species such as D. chameleon, D. erumenatum, D. candidum, etc., using 10 randomly amplified polymorphic DNA (RAPD) primers. Of these Dendrobium studied, 14 are native in Taiwan, 2 from the Philippinges and 3 from mainland China. The phalaenopsis aphrodite seedlings derived from tissue culture were included for comparison. The plant morphology and biological traits were also investigated to study ecological effects on genetic composition. The plant growth was influenced by its epiphytic site and in

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49

Zhang, Cai-Yuan, and 張財源. "Using RAPO(random amplified polymorphic DNA) fragment analysis in brassica." Thesis, 1994. http://ndltd.ncl.edu.tw/handle/99021615354849068420.

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50

Lopez-Buesa, Otilia. "Randomly amplified polymorphic DNA markers as tags for the major virus-resistance genes in cucumber." 1994. http://catalog.hathitrust.org/api/volumes/oclc/33036230.html.

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Thesis (M.S.)--University of Wisconsin--Madison, 1994.<br>Typescript. eContent provider-neutral record in process. Description based on print version record. Includes bibliographical references (leaves 10-12).

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