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Tesi sul tema "Recombinant proteins Analysis"

Autore: Grafiati

Pubblicato: 4 giugno 2021

Ultima modifica: 30 gennaio 2023

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1

Lee, Jae-Yong. "Expression, purification and interaction analysis of recombinant SRB proteins." Thesis, Imperial College London, 2003. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.407809.

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2

Kotlarski, Nicholas. "Process-scale renaturation of recombinant proteins from inclusion bodies /." Title page, contents and summary only, 1998. http://web4.library.adelaide.edu.au/theses/09PH/09phk87.pdf.

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3

Kepple, Kevin V. "Analysis of the binding mechanisms and cellular targets of peptide inhibitors that block site-specific recombination in vitro /." Diss., Connect to a 24 p. preview or request complete full text in PDF formate. Access restricted to UC campuses, 2006. http://wwwlib.umi.com/cr/ucsd/fullcit?p3208620.

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4

Castilho, Alexandra Marina Machado Ferreira. "Molecular cytogenic analysis of recombinant chromosomes in wheat - Aegilops umbellulata lines." Thesis, University of East Anglia, 1995. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.296341.

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5

Rauf, Femina. "Chimeric and Recombinant Protein Reagents for Cellular Analysis and Immunoassays." Diss., The University of Arizona, 2011. http://hdl.handle.net/10150/145441.

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Development of chimeric, recombinant peptides, proteins and enzymes expands the availability of protein/enzyme–based tools for cellular analysis and new assay platforms. Ideal protein reagents for cellular analysis must translocate into a variety of cells with minimum cell damage, retain stability and biological activity within the cell during analysis, and provide a reliable, measurable signal. This work focused on development, characterization and utilization of chimeric recombinant peptide, protein and enzyme reagents for cellular analysis and immunoassays. A cell-penetrating, fluorescent p

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6

Larsen, Sasha Ellen Marie. "Characterization of components that increase secretion of recombinant proteins in pichia pastoris." Scholarly Commons, 2011. https://scholarlycommons.pacific.edu/uop_etds/769.

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Pichia pastoris is a methylotrophic yeast that is commonly used for its ability to express and secrete heterologous proteins. However, some proteins are not readily secreted in P pastoris and so adjustments in the secretion pathway must be made in order to achieve secretion. The Lin-Cereghino lab previously developed mutant strains using restriction enzyme-mediated integration that enabled P pastoris to secrete Pgalactosidase at higher levels than the wild type strain. This study focuses on characterizing the random pREMI-Z mutations in the genomic DNA and examining their secretory phenotype,

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7

Carre, Heather Emily. "Expression and analysis of recombinant mycoplasma hyponeumoniae proteins as potential subunit vaccine candidates." Thesis, Royal Veterinary College (University of London), 2009. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.522182.

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8

Moore, Shona. "An analysis of the structure and function of malarial Duffy-binding-like protein domains using recombinant fusion proteins." Thesis, University of Warwick, 2016. http://wrap.warwick.ac.uk/86933/.

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Duffy-binding-like domains are present in two potential malaria vaccine candidates. Located on the merozoite surface, MSPDBL1 and MSPDBL2 have been implicated in erythrocyte invasion and identified as targets of natural immunity. Merozoite DBL domains have been shown to bind the Fc region of natural IgM. This is characteristic of several PfEMP1s, and is also well documented in bacteria, viruses and other parasites, where it is thought to prevent specific binding of the more deadly IgG antibodies. We have developed a mammalian expression system to produce merozoite DBL domains as Fc fusion prot

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9

Prasad, Alpana. "Immune function and structural analysis of recombinant bovine conglutinin and human lung surfactant protein-D." Thesis, University of Oxford, 2000. http://ora.ox.ac.uk/objects/uuid:f9a5ae66-4ed0-4bdf-90eb-c873ca44147d.

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Recognition of sugar moieties on the surface of microorganisms is one of the ways the body distinguishes potential pathogens from self-cells. The sugarbinding proteins, lectins, mediate this recognition role of the first line of defence against infections, preceding the antibody-mediated (adaptive) immune response. Collectins are calcium-dependent carbohydrate-binding proteins that have been implicated in innate immunity. Bovine conglutinin (BC) and lung surfactant protein-D (SP-D), belong to the family of 'collectins' which are characterised by four domains: an N-terminal cysteine-rich region

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10

Alodailah, Sattam Sonitan. "The Generation of Recombinant Zea mays Spastin and Katanin Proteins for In Vitro Analysis." Thesis, University of North Texas, 2017. https://digital.library.unt.edu/ark:/67531/metadc1062897/.

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Plant microtubules play essential roles in cell processes such as cell division, cell elongation, and organelle organization. Microtubules are arranged in highly dynamic and ordered arrays, but unlike animal cells, plant cells lack centrosomes. Therefore, microtubule nucleation and organization are governed by microtubule-associated proteins, including a microtubule-severing protein, katanin. Mutant analysis and in vitro characterization has shown that the highly conserved katanin is needed for the organization of the microtubule arrays in Arabidopsis and rice as well as in a variety of anima

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11

Wu, Xiaoqiu. "Functional genomics at the interface of protein expression and biophysical analysis /." Stockholm, 2004. http://diss.kib.ki.se/2004/91-7349-772-X.

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12

Wangsa-Wirawan, Norbertus Djajasantosa. "Physicochemical properties of protein inclusion bodies." Title page, contents and introduction only, 1999. http://web4.library.adelaide.edu.au/theses/09PH/09phw2465.pdf.

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Bibliography: leaves 182-198. Improvements in the current production system of inclusion bodies and the downstream processing sequence are essential to maintain a competitive advantage in the market place. Optimisation of fermentation is considered to improve production yield; then flotation as a possible inclusion body recovery method.

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13

Porter, Alison J. "Analysis of the efficiency of selecting GS-CHO cell lines for cGMP manufacture of recombinant proteins." Thesis, University of Manchester, 2010. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.692544.

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The market size for biotherapeutics is growing rapidly and the hunt is always on for the next blockbuster drug. A wide range of cell phenotypes are obtained during the process of generating mammalian cell lines suitable for the production of a biotherapeutic such as a monoclonal antibody. A strategy is therefore required for the selection of a cell line with ’desirable’ characteristics, from a population of closely related cell lines, for the successful manufacture of the biotherapeutic. As this process is included in the sequence of activities which determines the minimum time to start cGMP m

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14

Choi, Leo. "Analysis of the effects of spatial localisation of transgenes on expression of recombinant proteins in CHO-DG44 cells." Thesis, University of Manchester, 2012. https://www.research.manchester.ac.uk/portal/en/theses/analysis-of-the-effects-of-spatial-localisation-of-transgenes-on-expression-of-recombinant-proteins-in-chodg44-cells(76d0a965-9c12-4745-a6a9-ada226dfe2a0).html.

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Chinese hamster ovary cell lines are commonly used as host for production of recombinant protein both in research and in the biotech industry. Recombinant cell lines are generated through random integration of multi-cistronic plasmid vector containing the genes of interest and selection marker gene into the host genome. The recombinant cell lines require several rounds of limiting clonal dilution to isolate stable high expressing clones. Stable high expression of the genes of interest is a rare and desired trait in recombinant clonal cell lines. Despite being clonal, cell lines eventually beco

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15

Greenham, Trevor. "Pointillism in Plant Systems Biology: I. Proteomic Analysis of Plant Exosome-like Particles II. Amyloplast-binding Puroindoline Fusion Proteins for Recombinant Protein Expression." Thesis, Université d'Ottawa / University of Ottawa, 2019. http://hdl.handle.net/10393/39647.

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Expanding upon our understanding of plant defense is critical, particularly with the perilous threats of climate change and overpopulation to our food security, health and well-being. In this study, we focused on plant defense using two distinct approaches. First, we performed a proteomic analysis of plant exosome-like nanoparticles in order to elucidate their defense related protein cargo. Secondly, we used a wheat antimicrobial protein, puroindoline, as a fusion partner for the expression of recombinant proteins in rice endosperm. Plant exosome-like nanoparticles (ELP) were isolated from fr

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16

Pakkanen, O. (Outi). "Assembly and secretion of recombinant human collagens and gelatins in the yeast Pichia pastoris, and generation and analysis of knock-out mice for collagen prolyl 4-hydroxylase type I." Doctoral thesis, University of Oulu, 2006. http://urn.fi/urn:isbn:9514281047.

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Abstract Collagen molecules consist of three polypeptide chains that are coiled around each other to form a triple-helical structure. The formation of stable collagen triple helices requires the hydroxylation of proline residues catalyzed by collagen prolyl 4-hydroxylases (C-P4H). Vertebrate C-P4H is an ER-resident enzyme that consists of two catalytically active α subunits and two β subunits. Production of recombinant human collagen and gelatin could have numerous medical and industrial applications, but most recombinant systems lack the C-P4H activity. The yeast Pichia pastoris has been succ

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17

Suzuki, Noriaki. "Applications of time-of-flight secondary ion mass spectrometry (TOF-SIMS) and x-ray photoelectron spectroscopy (XPS) to study interactions of genetically engineered proteins with noble metal films /." Thesis, Connect to this title online; UW restricted, 2006. http://hdl.handle.net/1773/10618.

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18

Coelho, Tatiane Maldonado. "Comparação da atividade biológica e da glicosilação da gonadotrofia coriônica equina recombinante (reCGβα) expressa em duas linhagens celulares de mamíferos visando à geração de um biofármaco." Universidade de São Paulo, 2014. http://www.teses.usp.br/teses/disponiveis/46/46131/tde-20072015-133508/.

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Atualmente, o Brasil encontra-se na privilegiada posição de maior produtor e exportador mundial de carne bovina, tornando a pecuária uma das atividades nacionais mais importantes e rentáveis. Este dado enfatiza a importância de pesquisa e desenvolvimento em reprodução bovina, especialmente em hormônios estimuladores da ovulação, tais como a gonadotrofina coriônica equina (eCG). Os produtos comerciais à base de eCG comercialmente disponíveis são purificados a partir do sangue de éguas gestantes, apresentando variabilidade de lote para lote e presença de contaminantes. Estes fatos, juntamente co

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19

Murakaeva, Asiya. "Structure, evolution and expression of the duplicated growth hormone genes of common carp (Cyprinus carpio)." Doctoral thesis, Humboldt-Universität zu Berlin, Mathematisch-Naturwissenschaftliche Fakultät I, 2009. http://dx.doi.org/10.18452/15982.

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Der Karpfen, Cyprinus carpio, ist eine tetraploide Fischart aus der Familie Cyprinidae, die vor 20-50 Mio Jahren entstanden ist. Das Ziel der vorliegenden Arbeit war der Versuch, die funktionelle Rolle der duplizierten GH Gene des Karpfens durch das Studium ihrer Struktur, Evolution und Expression zu verstehen. Die Introns des zweiten GH Gens des Karpfens wurden erstmalig sequenziert und Sequenzvergleiche der kodierenden und nicht-kodierenden Bereiche von Allelen beider GH Gene wurden vorgenommen. Eine phylogenetische Analyse wurde durchgefuhrt, um die Beziehungen der GH Gene des Karpfens zu d

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20

Reid, Helen Isobel. "Molecular and immunological analysis of recombinant Bacteroides forsythus heat shock protein 60." Thesis, University of Glasgow, 1999. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.321982.

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21

Riley, Aidan. "Analysis and exploitation of GPI anchors in the expression of recombinant protein biopharmaceuticals." Thesis, University of Sheffield, 2012. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.578056.

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In this thesis we show the generation and characteriation of specific GPI-anchored cell surface markers. The creation of these constructs has also allowed the quantification of levels of GPI linked protein shedding into the cell-culture media. Experiments presented in the thesis also show that the addition of a GPI anchor to Growth hormone (GH) appears to reduce expression in the media and that these molecules retain the GPI moiety. We speculated that anchoring cytokine ligands to the cell surface might also modulate receptor mediated signalling. To test the effect of GPI modification on cytok

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22

Bruggeman, Andrew. "Generation of fluorescent recombinant listeriolysin O toxin for analysis of interactions with host protein." Connect to resource, 2008. http://hdl.handle.net/1811/32222.

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23

Perry, William B. "Global transcriptional analysis of an Escherichia coli recombinant protein process during hypoxia and hyperoxia." Thesis, Massachusetts Institute of Technology, 2004. http://hdl.handle.net/1721.1/28666.

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Thesis (Ph. D.)--Massachusetts Institute of Technology, Dept. of Chemical Engineering, 2004.<br>Includes bibliographical references (p. 289-302).<br>(cont.) The effects of recombinant protein production were observed through expression analysis of induced, uninduced, and Empty-Vector cultures. As expected, recombinant α₁AT production led to increased expression of heat-shock genes, including proteases and chaperones that are known to be involved in α₁AT degradation. Based on expression analysis data, production of recombinant α₁AT also resulted in catabolite repression and decreased amino acid

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24

Yan, Junhong. "DNA-Assisted Immunoassays for High-Performance Protein Analyses." Doctoral thesis, Uppsala universitet, Institutionen för immunologi, genetik och patologi, 2014. http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-236591.

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Proteins play important roles in most cellular functions, such as, replication, transcription regulation, signal transduction, for catalyzing chemical reaction, etc. Technologies developed to identify proteins rely either on observing their own properties such as charge, size, mass to charge ratio or sequence composition; or on using affinity reagents that recognize specific protein targets. Immunoassays utilizing functionalized affinity reagents are powerful for targeted proteomics. Among them, DNA-assisted immunoassays in which affinity reagents are labeled with DNA molecules, offer some uni

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25

Cuccinello, Sarah Elizabeth. "Analysis of Ahr Expression and Stability in a Recombinant Yeast Model System." Scholar Commons, 2011. http://scholarcommons.usf.edu/etd/3053.

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The aryl hydrocarbon receptor (Ahr) and the aryl hydrocarbon receptor nuclear translocator (Arnt) are well characterized bHLH-PAS transcription factors shown to regulate expression of xenobiotic metabolism genes. Extensive study has shown that upon treatment with certain aromatic hydrocarbons, mammalian cells rapidly activate the Ahr signaling pathway in order to stimulate gene expression and attempt to metabolize the xenobiotic compounds. It has been shown that after DNA-binding, the Ahr but not the Arnt protein, is quickly eliminated from the nuclear compartment thereby attenuating the dose

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26

Celik, Akdur Eda. "Bioprocess Development For Therapeutical Protein Production." Phd thesis, METU, 2008. http://etd.lib.metu.edu.tr/upload/3/12610236/index.pdf.

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In this study, it was aimed to develop a bioprocess using the Pichia pastoris expression system as an alternative to the mammalian system used in industry, for production of the therapeutically important glycoprotein, erythropoietin, and to form stoichiometric and kinetic models. Firstly, the human EPO gene, fused with a polyhistidine-tag and factor-Xa protease target site, in which cleavage produces the native termini of EPO, was integrated to AOX1 locus of P. pastoris. The Mut+ strain having the highest rHuEPO production capacity was selected. The glycosylation profile of rHuEPO was charact

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27

Grosse-Holz, Friederike. "Proteases and inhibitors in the interaction between Nicotiana benthamiana and Agrobacterium tumefaciens : systematic analysis and emerging solutions for molecular farming." Thesis, University of Oxford, 2017. https://ora.ox.ac.uk/objects/uuid:6146918c-3749-4604-88fa-01d426e4a817.

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Nicotiana benthamiana is now an established platform for molecular farming, the production of biopharmaceuticals in plants. Infiltration with Agrobacterium tumefaciens (agroinfiltration) is commonly used to transiently express one or multiple transgenes in N. benthamiana leaves. Agroinfiltrated N. benthamiana is a flexible and scalable recombinant protein (RP) production platform, but is impeded by low RP yields. Plant proteases can degrade RPs and thus limit RP accumulation. To inform, design and implement strategies for enhancing RP accumulation, I present four papers about proteases and pro

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28

Simila, Henry Allan. "Recombinant production and in silico analysis of the Androgen receptor ligand binding domain." Thesis, Queensland University of Technology, 2006. https://eprints.qut.edu.au/16349/1/Henry_Simila_Thesis.pdf.

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The androgen receptor (AR) fulfils important roles for both sexes. By mediating the biological function of androgens, the AR has remained the target for endocrine therapies treating prostate cancer. The AR also determines the effectiveness of medroxyprogesterone acetate (MPA) in treating AR positive breast cancer. Every man will be affected by prostate cancer if he lives long enough. Prostate cancer continues to be a leading cause of death for males despite research into this cancer covering more than 60 years since Huggins' seminal 1941 study showing that androgens play a key role in this

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Simila, Henry Allan. "Recombinant production and in silico analysis of the Androgen receptor ligand binding domain." Queensland University of Technology, 2006. http://eprints.qut.edu.au/16349/.

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The androgen receptor (AR) fulfils important roles for both sexes. By mediating the biological function of androgens, the AR has remained the target for endocrine therapies treating prostate cancer. The AR also determines the effectiveness of medroxyprogesterone acetate (MPA) in treating AR positive breast cancer. Every man will be affected by prostate cancer if he lives long enough. Prostate cancer continues to be a leading cause of death for males despite research into this cancer covering more than 60 years since Huggins' seminal 1941 study showing that androgens play a key role in this

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30

Khan, Obaid Yusuf. "Construction of recombinant adenoviruses encoding skeletal troponin C protein and expression analyses in transduced cardiac myocytes." Thesis, University of Glasgow, 1998. http://theses.gla.ac.uk/5438/.

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Troponin C is a regulatory protein of the myofilament which binds to calcium to trigger the process of contraction. This protein exists in two isoforms, skeletal and cardiac, which are spatially and temporally regulated. Work in this project builds the primary stage of a long-term project, for using the gene transfer method to overexpress the skeletal isoform of Troponin C in cardiomyocytes. The long-term aim is to achieve complete or partial substitution of the native cardiac isoform and study the effects on contractile force produced, in normal and ischemic cardiomyocytes, both in vitro and

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31

Sun, Qian. "Molecular analysis of factors involved in regulation of protein expression by recombinant Chinese hamster ovary (CHO) cells." Thesis, University of Manchester, 2009. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.505481.

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The demand for approved biopharmaceuticals products from animal cell culture have been rapidly increasing since last few decades. Many efforts have been made on process refining in the past, and now, works are focusing on the selection or generation of high producer cell lines. It was therefore very important to understanding the molecular mechanisms underlying the existing high producing cell lines and to identify cellular regulators responsible for enhanced productivity.

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Pereira, Luiz Augusto. "Identificação proteômica, seqüência de nucleotídeos, expressão heteróloga e reatividade imunológica da triose fosfato isomerase de Paracoccidioides brasiliensis." Universidade Federal de Goiás, 2004. http://repositorio.bc.ufg.br/tede/handle/tede/3778.

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Submitted by Erika Demachki (erikademachki@gmail.com) on 2014-12-15T16:53:37Z No. of bitstreams: 2 Dissertação - Luiz Augusto Pereira - 2004.pdf: 6566857 bytes, checksum: 144c5557e8a138c6a21c528ad0262aa8 (MD5) license_rdf: 23148 bytes, checksum: 9da0b6dfac957114c6a7714714b86306 (MD5)<br>Approved for entry into archive by Erika Demachki (erikademachki@gmail.com) on 2014-12-15T17:01:19Z (GMT) No. of bitstreams: 2 Dissertação - Luiz Augusto Pereira - 2004.pdf: 6566857 bytes, checksum: 144c5557e8a138c6a21c528ad0262aa8 (MD5) license_rdf: 23148 bytes, checksum: 9da0b6dfac957114c6a7714714b86306 (MD5)

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Neubauer, A. (Antje). "Expression and analysis of recombinant human collagen prolyl 4-hydroxylase in E. coli and optimization of expression." Doctoral thesis, University of Oulu, 2006. http://urn.fi/urn:isbn:9514281063.

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Abstract Collagen prolyl 4-hydroxylase (C-P4H) plays a central role in the biosynthesis of collagens by hydroxylating proline residues. The enzyme has been a subject of intense interest as a target enzyme for drug development. The recombinant expression of human C-P4H in prokaryotes has not yet been described. This work reports on the development of an expression system for human C-P4H in E. coli. The vertebrate C-P4H enzymes are α2β2 tetramers, consisting of two β subunits which are identical to protein disulphide isomerase (PDI), aside from the two α subunits which have the catalytic activi

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34

Wandrey, Georg Benjamin [Verfasser], Jochen [Akademischer Betreuer] Büchs, and Jörg [Akademischer Betreuer] Pietruszka. "Light-mediated control and analysis of recombinant protein production in microscale cultivations / Georg Benjamin Wandrey ; Jochen Büchs, Jörg Pietruszka." Aachen : Universitätsbibliothek der RWTH Aachen, 2017. http://d-nb.info/1161809112/34.

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Myers, Andrew Ross. "Cloning, Expression, and Sequence Analysis of Camelysin, a Zinc Metalloprotease from Bacillus anthracis and B. cereus." [Tampa, Fla.] : University of South Florida, 2005. http://purl.fcla.edu/fcla/etd/SFE0001218.

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Dolata, Katarzyna [Verfasser], Katharina [Gutachter] Riedel, and Wolfgang [Gutachter] Liebl. "Proteomics-based analysis of stress responses during recombinant protein production in Escherichia coli / Katarzyna Dolata ; Gutachter: Katharina Riedel, Wolfgang Liebl." Greifswald : Universität Greifswald, 2019. http://d-nb.info/1194162835/34.

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Dolata, Katarzyna Verfasser], Katharina [Gutachter] Riedel, and Wolfgang [Gutachter] [Liebl. "Proteomics-based analysis of stress responses during recombinant protein production in Escherichia coli / Katarzyna Dolata ; Gutachter: Katharina Riedel, Wolfgang Liebl." Greifswald : Universität Greifswald, 2019. http://nbn-resolving.de/urn:nbn:de:gbv:9-opus-29819.

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Jokela, M. (Maarit). "Replicative DNA polymerase associated B-subunits." Doctoral thesis, University of Oulu, 2004. http://urn.fi/urn:isbn:9514274814.

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Abstract Replicative DNA polymerases (pols) synthesize chromosomal DNA with high accuracy and speed during cell division. In eukaryotes the process involves three family B pols (α, δ, ε), whereas in Archaea, two types of pols, families B and D, are involved. In this study the B-subunits of replicative pols were analysed at the DNA, RNA and protein levels. By cloning the cDNAs for the B-subunits of human and mouse pol ε we were able to show that the encoded proteins are not only homologous to budding yeast pol ε, but also to the second largest subunit of pol α. Later studies have revealed that

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Danh, Tu Thien. "ANALYSIS OF CHROMATIN ACCESSIBILITY OF THE HUMAN C-MYC REPLICATION ORIGIN." Wright State University / OhioLINK, 2015. http://rave.ohiolink.edu/etdc/view?acc_num=wright1449845546.

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Mao, Liangyan. "Assessment of Bone Regeneration in a Rat Femur Defect Model Following Recombinant Human Bone Morphogenetic Protein 2 Delivery from Keratin Hydrogels with Tunable Rates of Degradation: Micro-CT Analysis and Histology." Miami University / OhioLINK, 2016. http://rave.ohiolink.edu/etdc/view?acc_num=miami148068386349526.

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St, Pierre Liam Daniel. "Identification and comparative analysis of novel factors from the venom gland of the coastal taipan (Oxyuranus scutellatus) and related species." Thesis, Queensland University of Technology, 2005. https://eprints.qut.edu.au/16677/1/Liam_St_Pierre_Thesis.pdf.

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Abstract (sommario):

Snake venoms are a complex mixture of polypeptide and other molecules that adversely affect multiple homeostatic systems within their prey in a highly specific and targeted manner. Amongst the most potently toxic venoms in the world are those of the Australian venomous snakes, which belong almost exclusively to the elapid family. Their venoms posses a number of unique properties by which they target the mammalian cardiovascular and neuromuscular systems and are the focus for the identification of novel pharmacologically interesting compounds which may be of diagnostic or therapeutic benefit. A

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St, Pierre Liam Daniel. "Identification and comparative analysis of novel factors from the venom gland of the coastal taipan (Oxyuranus scutellatus) and related species." Queensland University of Technology, 2005. http://eprints.qut.edu.au/16677/.

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Abstract (sommario):

Snake venoms are a complex mixture of polypeptide and other molecules that adversely affect multiple homeostatic systems within their prey in a highly specific and targeted manner. Amongst the most potently toxic venoms in the world are those of the Australian venomous snakes, which belong almost exclusively to the elapid family. Their venoms posses a number of unique properties by which they target the mammalian cardiovascular and neuromuscular systems and are the focus for the identification of novel pharmacologically interesting compounds which may be of diagnostic or therapeutic benefit. A

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Nadkarni, Aditi A. "Functional analysis of the Rad51d (E233G) breast cancer associated polymorphism and a pharmacogenetic evaluation of RAD51D status." Connect to full text in OhioLINK ETD Center, 2008. http://rave.ohiolink.edu/etdc/view?acc%5Fnum=mco1222877984.

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Dissertation (Ph.D.)--University of Toledo, 2008.<br>"In partial fulfillment of the requirements for the degree of Doctor of Philosophy in Biomedical Sciences." Title from title page of PDF document. Bibliography: pages 73-77, 93-95, 109-111, 145-172.

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Rayon, Catherine. "La N-glycosylation chez les plantes. Etude d'une glycoprotéine modèle : la phytohémagglutinine." Rouen, 1998. http://www.theses.fr/1998ROUES007.

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La production de protéines d'intérêt thérapeutique dans des plantes transgéniques suppose que la cellule végétale soit capable d'effectuer les modifications post-traductionnelles, en particulier la N-glycosylation, indispensables à la biosynthèse d'une protéine biologiquement active et stable. Les données actuelles relatives à la N-glycosylation des protéines végétales étant encore parcellaires, nous avons cherché à réaliser une analyse comparée de la biosynthèse et la maturation des N-glycannes dans différents systèmes constitutifs du règne végétal et au sein de différents organes d'une même

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Abdallah, Bassim Violla. "Structural and functional analysis on GacS homodimeric histidine kinase reveals a non-canonical autokinase activity and new insights into the heterodimer partnership with RetS." Thesis, Aix-Marseille, 2020. http://www.theses.fr/2020AIXM0256.

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Pseudomonas aeruginosa (PA) est un pathogène qui infecte particulièrement les patients atteints de la mucoviscidose. PA provoque des infections aiguës et chroniques et alterne entre des modes de vie planctonique et sédentaire. Cette transition est principalement régulée par les systèmes à deux composants (TCS). Durant ma thèse, je me suis focalisée sur le TCS GacS/GacA, impliqué dans un réseau de signalisation multikinase. GacS est une histidine kinase (HK) et GacA est son régulateur de réponse (RR). La région cytoplasmique de GacS est composée des domaines HAMP, S-Hélice, H1, D1 et H2. Des te

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Lin, Han-Yun, and 林涵芸. "Immunogenic Analysis of the Recombinant Proteins of Mannheimia haemolytica." Thesis, 2009. http://ndltd.ncl.edu.tw/handle/11556401006999604581.

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碩士<br>國立屏東科技大學<br>動物疫苗科技研究所<br>97<br>Mannheimia (Pasteurella) haemolytica is a member of the Pasteurellaceae family, which is the major cause of acute fibrinous pneumonia in ruminants. The mortality is high and vaccination is the major strategy for controlling this disease. There is no effect vaccine to protect pneumonic pasteurellosis. It is urgency to develop new vaccine to prevent M. haemolytica infection and reduce the economical loss. The previous studies indicated that outer membrane proteins GS60 are the most important antigens and the leukotoxin (LKT) plays a major role in lung injury.

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Yeh, Po-Hsien, and 葉伯賢. "Antigenic Analysis of Actinobacillus pleuropeumoninae Recombinant Apx Toxin Proteins." Thesis, 2010. http://ndltd.ncl.edu.tw/handle/20921799649786339545.

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碩士<br>國立屏東科技大學<br>動物疫苗科技研究所<br>98<br>Actinobacillus pleuropeumoninae (A.p.) is a type of hemolytic Gram-negative bacteria, which includes a total of 15 serotypes, and can cause fibrinonecrotic and hemorrhagic pleuropneumonia, resulting in serious economic loss in farms. The pathogenic factors of A.p. are capsular polysaccharides, lipopolysaccharides, outer membrane proteins (OMP), adsorption factors, and exotoxins. Actinobacillus pleuropneumoniae toxins (Apxs) belong to RTX (repeat in the structural toxin) family, are exotoxins and important virulence factors with immunogenicity, is more effic

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Kim, Hyun-Eui. "Biochemical analysis of apoptosome formation." 2006. http://www4.utsouthwestern.edu/library/ETD/etdDetails.cfm?etdID=215.

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Kotlarski, Nicholas. "Process-scale renaturation of recombinant proteins from inclusion bodies / by Nicholas Kotlarski." Thesis, 1998. http://hdl.handle.net/2440/19259.

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Bibliography: leaves 215-236.<br>x, 249 leaves : ill. ; 30 cm.<br>Scale-up of a biochemical process involving expression of an Insulin-like Growth Factor-I analogue (LongR3IGF-I) as inclusion bodies within the bacterium Escherichia coli has been investigated. The principal focus was directed to the operation of refolding wherein the biological potency of the protein is imparted.<br>Thesis (Ph.D.)--University of Adelaide, Dept. of Chemical Engineering, 1998?

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Zhang, Yuan Heidi. "Mass spectrometric analysis of proteins and peptides : elucidation of the folding pathways of recombinant human macrophage colony stimulating factor beta." Thesis, 2002. http://hdl.handle.net/1957/37223.

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Recombinant human macrophage colony stimulating factor beta (rhm-CSFβ) is a glycoprotein that stimulates the proliferation, differentiation and survival of cells belonging to the monocyte-macrophage lineage. It contains nine inter-subunit and intra-subunit disulfide bonds and represents an excellent model system for studying disulfide bond formation during protein folding because the assembly of its monomeric subunits and the maturation of its biological activity depend on the progressive formation of the correct disulfide structure during in vitro folding. Knowledge obtained from these studie

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