Bibliografie tematiche / Strand displacement amplification

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Letteratura scientifica selezionata sul tema "Strand displacement amplification"

Autore: Grafiati

Pubblicato: 4 giugno 2021

Ultima modifica: 6 febbraio 2022

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Articoli di riviste sul tema "Strand displacement amplification"

Walker, G. T. "Empirical aspects of strand displacement amplification." Genome Research 3, no. 1 (August 1, 1993): 1–6. http://dx.doi.org/10.1101/gr.3.1.1.

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Seckinger, D. "Strand displacement amplification and fluorescence polarization." Clinical Chemistry 42, no. 10 (October 1, 1996): 1720. http://dx.doi.org/10.1093/clinchem/42.10.1720.

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Walker, G. Terrance, Melinda S. Fraiser, James L. Schram, Michael C. Little, James G. Nadeau, and Douglas P. Malinowski. "Strand displacement amplification—an isothermal,in vitroDNA amplification technique." Nucleic Acids Research 20, no. 7 (1992): 1691–96. http://dx.doi.org/10.1093/nar/20.7.1691.

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Mullor Ruiz, Ismael, Jean-Michel Arbona, Amitkumar Lad, Oscar Mendoza, Jean-Pierre Aimé, and Juan Elezgaray. "Connecting localized DNA strand displacement reactions." Nanoscale 7, no. 30 (2015): 12970–78. http://dx.doi.org/10.1039/c5nr02434j.

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Shi, Chao, Qi Liu, Cuiping Ma, and Wenwan Zhong. "Exponential Strand-Displacement Amplification for Detection of MicroRNAs." Analytical Chemistry 86, no. 1 (December 18, 2013): 336–39. http://dx.doi.org/10.1021/ac4038043.

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Spargo, C. A., M. S. Fraiser, M. Van Cleve, D. J. Wright, C. M. Nycz, P. A. Spears, and G. T. Walker. "Detection ofM. tuberculosisDNA using Thermophilic Strand Displacement Amplification." Molecular and Cellular Probes 10, no. 4 (August 1996): 247–56. http://dx.doi.org/10.1006/mcpr.1996.0034.

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Joneja, Aric, and Xiaohua Huang. "Linear nicking endonuclease-mediated strand-displacement DNA amplification." Analytical Biochemistry 414, no. 1 (July 2011): 58–69. http://dx.doi.org/10.1016/j.ab.2011.02.025.

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Hellyer, Tobin J., and James G. Nadeau. "Strand displacement amplification: a versatile tool for molecular diagnostics." Expert Review of Molecular Diagnostics 4, no. 2 (March 2004): 251–61. http://dx.doi.org/10.1586/14737159.4.2.251.

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Li, Yubin, Sheng Liu, Zike Zhao, Yuner Zheng, and Zirui Wang. "Binding induced strand displacement amplification for homogeneous protein assay." Talanta 164 (March 2017): 196–200. http://dx.doi.org/10.1016/j.talanta.2016.11.047.

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Lee, Chang Yeol, Hansol Kim, Hyo Yong Kim, Ki Soo Park, and Hyun Gyu Park. "Fluorescent S1 nuclease assay utilizing exponential strand displacement amplification." Analyst 144, no. 10 (2019): 3364–68. http://dx.doi.org/10.1039/c9an00300b.

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Più fonti

Tesi sul tema "Strand displacement amplification"

Morant, Nick. "Novel thermostable DNA polymerases for isothermal DNA amplification." Thesis, University of Bath, 2015. https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.667735.

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Abstract (sommario):

DNA polymerases play a fundamental role in the transmission and maintenance of genetic information and have become an important in vitro diagnostic and analytical tool. The Loop-mediated isothermal DNA amplification (LAMP) method has major applications for disease and pathogen detection and utilises the unique strand-displacement activity of a small group of thermostable DNA polymerases. The Large (Klenow-like) Fragment of Geobacillus stearothermophilus DNA polymerase I (B.st LF Pol I) currently serves as the enzyme of choice for the majority of these isothermal reactions, with few alternative

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Raikar, Sanjeev Vencu. "Protoplast fusion of Lolium perenne and Lotus corniculatus for gene introgression." Phd thesis, Lincoln University. Bio-Protection and Ecology Division, 2007. http://theses.lincoln.ac.nz/public/adt-NZLIU20080214.105406/.

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Protoplast fusion of Lolium perenne and Lotus corniculatus for gene introgression by Sanjeev V. Raikar Lolium perenne is one of the most important forage crops globally and in New Zealand. Lotus corniculatus is a dicotyledonous forage that contains valuable traits such as high levels of condensed tannins, increased digestibility, and high nitrogen fixing abilities. However, conventional breeding between these two forage crops is impossible due to their markedly different taxonomic origin. Protoplast fusion (somatic hybridisation) provides an opportunity for gene introgression between these two

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Benmohamed, Fayçal. "Contribution au développement d'une méthode numérique pour étudier des structures planaires hyperfréquences." Thesis, Lyon, 2020. http://www.theses.fr/2020LYSES004.

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Le travail proposé est le résultat d’une collaboration entre le laboratoire Physique de la matière condensée et nanosciences de Monastir, le laboratoire Laplace de Toulouse et le laboratoire Hubert Curien de Saint-Étienne. Il s’inscrit dans un projet visant à étudier théoriquement la propagation des ondes électromagnétiques dans des dispositifs passifs à base du matériau magnétique anisotrope. La présence d’une couche magnétique dans telle structure a de nombreux avantages : non réciprocité́, performances intéressantes, isolation importante, …Plus précisément, c’est la fabrication des disp

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Raikar, S. V. "Protoplast fusion of Lolium perenne and Lotus corniculatus for gene introgression." Diss., Lincoln University, 2007. http://hdl.handle.net/10182/301.

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Abstract (sommario):

Lolium perenne is one of the most important forage crops globally and in New Zealand. Lotus corniculatus is a dicotyledonous forage that contains valuable traits such as high levels of condensed tannins, increased digestibility, and high nitrogen fixing abilities. However, conventional breeding between these two forage crops is impossible due to their markedly different taxonomic origin. Protoplast fusion (somatic hybridisation) provides an opportunity for gene introgression between these two species. This thesis describes the somatic hybridisation, the regeneration and the molecular analysis

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Chang, Wen-Hsin, and 張文馨. "DNA-based Hydrogel Microcapsules Coupled with Strand-Displacement Amplification for the Detection of miR-141." Thesis, 2019. http://ndltd.ncl.edu.tw/handle/w666xb.

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Capitoli di libri sul tema "Strand displacement amplification"

Kroneis, Thomas, and Amin El-Heliebi. "Whole Genome Amplification by Isothermal Multiple Strand Displacement Using Phi29 DNA Polymerase." In Whole Genome Amplification, 111–17. New York, NY: Springer New York, 2015. http://dx.doi.org/10.1007/978-1-4939-2990-0_8.

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Hansen, Sören, Oumar Faye, Sabri S. Sanabani, Martin Faye, Susanne Böhlken-Fascher, Ousmane Faye, Amadou Alpha Sall, et al. "Zika Virus Amplification Using Strand Displacement Isothermal Method and Sequencing Using Nanopore Technology." In Methods in Molecular Biology, 123–36. New York, NY: Springer US, 2020. http://dx.doi.org/10.1007/978-1-0716-0581-3_11.

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"Strand Displacement Amplification (SDA)." In Encyclopedia of Medical Genomics and Proteomics, 1–4. CRC Press, 2004. http://dx.doi.org/10.1081/e-emgp-120020701.

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"Hyperbranched Strand Displacement Amplification." In Encyclopedia of Genetics, Genomics, Proteomics and Informatics, 939. Dordrecht: Springer Netherlands, 2008. http://dx.doi.org/10.1007/978-1-4020-6754-9_8035.

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Zeng, Lingwen, Omar Mukama, Xuewen Lu, Shilin Cao, and Donghai Lin. "Strand Displacement Amplification for Multiplex Detection of Nucleic Acids." In Modulating Gene Expression - Abridging the RNAi and CRISPR-Cas9 Technologies. IntechOpen, 2019. http://dx.doi.org/10.5772/intechopen.80687.

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Walker, G. T., C. A. Spargo, C. M. Nycz, J. A. Down, M. S. Dey, A. H. Walters, D. R. Howard, et al. "A Chemiluminescent DNA Probe Test Based on Strand Displacement Amplification." In Molecular Methods for Virus Detection, 329–49. Elsevier, 1995. http://dx.doi.org/10.1016/b978-012748920-9/50016-9.

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Clark, Duncan, and Mark Wilks. "Molecular Diagnostics." In Tutorial Topics in Infection for the Combined Infection Training Programme. Oxford University Press, 2019. http://dx.doi.org/10.1093/oso/9780198801740.003.0018.

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Abstract (sommario):

Molecular diagnostics in infection generally relate to the detection and/ or characterization of nucleic acid sequences of infectious agents in clinical samples which are used to provide: ● A laboratory diagnosis. ● A means of monitoring patients at risk of developing disease caused by a particular infection. ● A method to predict through genotypic analysis the susceptibility or resistance to appropriate treatments. ● A measurement of the response to therapy. A few key laboratory techniques underpin the majority of molecular diagnostic tests that are currently used in the field of infection, and include: ● Block-based polymerase chain reaction (PCR). ● Real-time PCR, including quantification. ● Strand displacement amplification. ● Transcription mediated amplification. ● DNA sequencing. These can be commercially sourced, which has the advantage of CE marking, or developed in-house, sometimes referred to as laboratory developed tests (LDTs). Whatever the source, the underlying principles are often the same and rigorous evaluation and validation is required for the adoption of any molecular test in the diagnostic laboratory. The majority of molecular diagnostic tests require the amplification of a specific DNA sequence and its subsequent detection by a variety of means. As such, small sequences of DNA from the infectious agent are amplified from a relatively low copy number in the clinical sample. For example, after thirty to forty cycles of PCR, a single copy of a sequence can theoretically be amplified to over a billion copies. This PCR product, commonly termed amplicon, can provide a template for any further testing with the same PCR test and therefore potentially act as a source for false positive results. Molecular diagnostic laboratories have requirements to keep the different stages of the molecular test separate and minimize the risk of amplicon contamination. Most facilities will have a ‘clean PCR laboratory’ that is used to store the clean reagents such as primers, probes, enzyme mastermixes, and no clinical samples, nucleic extracts, or amplification reactions are ever taken into this environment. Another laboratory is used for the nucleic acid extraction of the clinical samples and this environment is often used to set up the PCR reactions.

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Atti di convegni sul tema "Strand displacement amplification"

Tolliver, Laura, Xiaoning Jiang, and Tian-Bing Xu. "Piezoelectric Actuators With Active and Passive Frames." In ASME 2013 Conference on Smart Materials, Adaptive Structures and Intelligent Systems. American Society of Mechanical Engineers, 2013. http://dx.doi.org/10.1115/smasis2013-3064.

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Electromechanical actuators that generate large displacements, have large load capabilities, and demonstrate strong resonance characteristics are in great demand in the areas of precision positioning, active vibration control, and energy harvesting. Piezoelectric materials have been widely investigated for these applications because of their high energy density, quick response time, and relatively low driving voltages, but they demonstrate very small strain, typically about 0.1%. We present experimental and finite element results for two designs that use active and passive frames, respectively

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Kommepalli, Hareesh K. R., Han G. Yu, Srinivas A. Tadigadapa, Christopher D. Rahn, Susan Trolier-McKinstry, and Christopher L. Muhlstein. "Displacement and Blocking Force Modeling for Piezoelectric Uniflex Microactuators." In ASME 2008 International Design Engineering Technical Conferences and Computers and Information in Engineering Conference. ASMEDC, 2008. http://dx.doi.org/10.1115/detc2008-49897.

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Microactuators provide controlled motion and force for applications ranging from RF switches to rate gyros. Large amplitude response in piezoelectric actuators requires amplification of their small strain. This paper studies a uniflex microactuator that combines the strain amplification mechanisms of a unimorph and flexural motion to produce large displacement and blocking force. An analytical model is developed with three connected beams and a reflective symmetric boundary condition that predicts actuator displacement and blocking force as a function of the applied voltage. The model shows th

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Esteki, H., and A. Hasannia. "Multi-Objective Optimization of Piezoelectric Microactuator Using Genetic Algorithms." In ASME 2008 International Mechanical Engineering Congress and Exposition. ASMEDC, 2008. http://dx.doi.org/10.1115/imece2008-66909.

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In flex-tensional piezoactuators, due to the low displacement of piezostacks, a compliant mechanism is used to amplify displacement of piezostack. In this paper, optimization of a compliant mechanism with corner-filleted flexure hinges is carried out using real-coded genetic algorithms (GAs) to avoid trapping in local optimums. The objective functions are displacement amplification and stiffness of mechanism and design variables are cross-sectional size and material used. The constraints which are applied on mechanism are based on piezostack dimensions and manufacturing limits. Displacement am

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Kommepalli, Hareesh K. R., Christopher D. Rahn, and Srinivas A. Tadigadapa. "Optimization of Piezoelectric Uniflex Microactuators." In ASME 2009 International Design Engineering Technical Conferences and Computers and Information in Engineering Conference. ASMEDC, 2009. http://dx.doi.org/10.1115/detc2009-87594.

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Microactuators provide controlled motion and force for applications ranging from RF switches to rate gyros. Large amplitude response in piezoelectric actuators requires amplification of their small strain. This paper studies the performance of a uniflex actuator in terms of its displacement and blocking force compared to uniflex and flextensional actuators. A uniflex microactuator combines the strain amplification mechanisms of a unimorph and flexural motion to produce large displacement and blocking force. Analytical models for displacement and blocking force for all the three actuators are u

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Ueda, Jun, Thomas Secord, and H. Harry Asada. "Piezoelectric Cellular Actuators Using Nested Rhombus Multilayer Mechanisms." In ASME 2008 Dynamic Systems and Control Conference. ASMEDC, 2008. http://dx.doi.org/10.1115/dscc2008-2128.

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Piezoelectric ceramic material, such as Lead Zirconate Titanate (PZT), has large stress and bandwidth, but its extremely small strain, i.e. only 0.1%, has been a major bottleneck for broad applications. We have proposed a “nested rhombus” multi-layer mechanism for PZT actuators, which increases strain exponentially through its hierarchical cellular structure, for over 20% effective strain. To drive a large load, however, care must be taken in the design of the strain amplification structure. Through kinematic and static analysis this paper addresses how the output force and displacement are at

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Neal, Devin, and H. Harry Asada. "Design of Cellular Piezoelectric Actuators With High Blocking Force and High Strain." In ASME 2008 Dynamic Systems and Control Conference. ASMEDC, 2008. http://dx.doi.org/10.1115/dscc2008-2209.

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Preliminary design and analysis of a new concept for efficiently amplifying piezoelectric actuators are presented in this paper. Piezoelectric actuators, such as Lead Zirconate Titanate (PZT), have produced substantial stress at high bandwidth, but at very small strains on the order of 0.1%. This paper presents a new strain amplification design to be utilized as the first layer in the previously designed “nested rhombus” multi-layer mechanism. This mechanism produces substantial strain through exponentially increasing strain with each subsequent layer. However, the blocking force produced in p

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Torres, James, Devin Neal, and H. Harry Asada. "A PZT Array Actuator Using Buckling Strain Amplification and Preload Mechanisms." In ASME 2011 Dynamic Systems and Control Conference and Bath/ASME Symposium on Fluid Power and Motion Control. ASMEDC, 2011. http://dx.doi.org/10.1115/dscc2011-6070.

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Displacement amplification mechanisms have been a topic of research for piezoelectric actuators for decades to overcome their significantly small strain, but still utilize their high power density, force, and efficiency. This paper further analyzes a nonlinear buckling mechanism to improve its efficiency, defined as the ratio of mechanical work output of the buckling actuator to the mechanical work output of the PZT actuator, as well as, employing two methods, preload and loading conditions, that improve its work output per cycle. This is accomplished by running a numerical analysis of the geo

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Cui, Tao, Weibin Wang, Guangwen Liu, Tingxia Ma, and Wenqiang Tong. "Research on Mechanics Monitoring System of Long Distance Oil and Gas Pipeline." In 2008 7th International Pipeline Conference. ASMEDC, 2008. http://dx.doi.org/10.1115/ipc2008-64513.

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The paper establishes a comprehensive organizational framework and a mechanical Remote Monitoring System with SCM technology, electronic communicating technology, network technology, electronic measuring technology, which can be used for long-distance pipeline stress and strain, displacement and the pressure monitoring. The system complete data collection, amplification, conversion, transmission, storage and automatic measurement from acquisition in the pipeline surface resistance strain tablets with the pipeline deformation resistance changes with the contingency collection, data conversion p

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Zaiss, Curtis, and Swavik Spiewak. "Vibration Rectification and Thermal Disturbances in Ultra Precision Inertial Sensors." In ASME 2011 International Mechanical Engineering Congress and Exposition. ASMEDC, 2011. http://dx.doi.org/10.1115/imece2011-65518.

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Advanced inertial MEMS sensors facilitate achieving superb precision and resolution in measuring translational and rotational displacements, down to femtometers and milli-arcseconds. At present such performance is possible only in measurements of a very short duration, typically below 1 second. As this duration increases, the precision rapidly deteriorates. However, experimental accelerometers indicate the possibility of measurements with sub-micron precision for up to 30 seconds. For longer measurements, e.g., up to 5 minutes, the errors increase. However they still remain below 100 μm. The m

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Beirow, Bernd, Arnold Ku¨hhorn, and Sven Schrape. "A Discrete Model to Consider the Influence of the Air Flow on Blade Vibrations of an Integral Blisk Compressor Rotor." In ASME Turbo Expo 2008: Power for Land, Sea, and Air. ASMEDC, 2008. http://dx.doi.org/10.1115/gt2008-50613.

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The influence of the aerodynamic coupling in the forced response analysis of a HPC test-blisk is studied by means of a reduced order mechanical model. In the first step this equivalent blisk model (EBM) is derived based on a finite element analysis of the disk from design and an adjustment to experimentally determined blade alone frequencies in order to consider the real blade mistuning. Applying the EBM — so far not considering the air flow influence — to carry out forced response analyses due to a rotating excitation acting on the stationary blisk, a maximum blade displacement amplification

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