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Letteratura scientifica selezionata sul tema "Survival of lawsonia intracellularis"

Autore: Grafiati

Pubblicato: 4 giugno 2021

Ultima modifica: 20 febbraio 2023

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Articoli di riviste sul tema "Survival of lawsonia intracellularis"

Pereira, Carlos Eduardo Real, Talita Pilar Resende, Aníbal G. Armién, Ricardo Pereira Laub, Fabio Augusto Vannucci, Renato Lima Santos, Connie Jane Gebhart e Roberto Mauricio Carvalho Guedes. "Survival of Lawsonia intracellularis in porcine peripheral blood monocyte-derived macrophages". PLOS ONE 15, n. 7 (31 luglio 2020): e0236887. http://dx.doi.org/10.1371/journal.pone.0236887.

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Garkusha, S. E., e J. O. Prodoljak. "МАКРОСКОПІЧНІ ЗМІНИ ПРИ ПРОЛІФЕРАТИВНІЙ ЕНТЕРОПАТІЇ СВИНЕЙ". Scientific Messenger of LNU of Veterinary Medicine and Biotechnology 18, n. 3(70) (9 agosto 2016): 33–36. http://dx.doi.org/10.15421/nvlvet7008.

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Infectious diseases cause significant economic damage to the industry and one such disease is proliferative enteropathy.So scientists have estimated that, for example, in Australia, the losses amount to dollars 20 per 1 pig, and in the United States losses from proliferative enteropathy in pigs was dollars 20 million annually.Symptoms and signs of the disease are known to scientists for a long time. But only in 1974, scientists discovered a bacterium that multiplies rapidly abnormally in the cells, it has been called Lawsonia intracellularis. And it is proved that it is the causative agent of the disease, in 1993.Recently we discovered that the infection in pig farms tend to widespread. According to the results of serological tests in different countries of Lawsonia intracellularis it is 60 – 90% of the herd of swine. Other authors have reported that around the world are infected with this microorganism is from 30% to 100% of herds of pigs. Serological studies have established that Lawsonia intracellularis in the United States recorded in 96% of the herd of swine. This is facilitated by the low infectious dose of pathogen isolation from faeces of a large number of bacteria, sustained release of the pathogen in the faeces and survival of Lawsonia Intracellularis in the environment for at least 2 weeks.In our state proliferative enteropathy pigs hardly been studied. In Ukraine, proliferative enteropathy pigs was detected in 15 pig farms 7 areas. In Russia, the disease is called a new and poorly understood, but some scientists call it lavsoniosis.Therefore the aim of this study was to examine in more detail the macroscopic changes at proliferative enteropathy pigs.To achieve this goal the following objectives: to conduct mortem examination of dead pigs of proliferative enteropathy, examine macroscopic changes in the internal organs of the disease, and a detailed description of the macroscopic changes in the internal organs, which they were not previously documented.The material for the study were 10 corpses of pigs killed by proliferative enteropathy. Mortem examination was carried out in spinal position by partial evisceration in a common sequence at the Department of Pathological Anatomy of the National University of Life and Environmental Sciences of Ukraine.At postmortem macroscopic changes at autopsy proliferative enteropathy in pigs were the following: thickening of the walls of the caudal part of the jejunum, ileum, cecum and colon intestine, which was accompanied by a distinct narrowing of the lumen. Swollen lymph nodes were recorded hemorrhagic thin, blind and colon. In mild venous congestion and edema and dilation of the heart and congestive hyperemia of the liver.

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Tokach, Mike D., Jordan T. Gebhardt, Steve S. Dritz, Jason C. Woodworth, Joel M. DeRouchey e Robert D. Goodband. "394 Opportunities to enhance wean-to-finish pig survival". Journal of Animal Science 98, Supplement_4 (3 novembre 2020): 184. http://dx.doi.org/10.1093/jas/skaa278.339.

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Abstract Swine productivity as measured by total pigs born, growth rate, and efficiency has improved steadily over the last few decades. However, post-weaning mortality has not improved for several years. Estimates for post-weaning mortality are 3.6% for nursery, 4.1% for grow-finish, and 5.6% of pigs entering the wean-finish phase (USDA, 2015). The first step in improving wean-to-finish survival is to understand major causes. Post-weaning mortality can be categorized into non-infectious and infectious causes. Non-infectious factors can be classified into anatomic abnormalities, toxicity, animal factors, facility factors, nutritional inadequacies, season, and management factors. Important non-infectious mortality factors identified through literature review include birth weight, preweaning management, weaning age and weight, and season. Additionally, a low incidence but high consequence cause for individual pigs is abdominal organ torsion. Sodium ion or ionophore toxicosis or dietary imbalance due to feed formulation or manufacture error are infrequent, but can be high magnitude. Infectious factors can be categorized as respiratory disease caused by swine influenza virus, porcine reproductive and respiratory syndrome virus, Mycoplasma spp., Glasserella parasuis, and others; enteric disease caused by Lawsonia intracellularis, Brachyspira spp; or systemic disease caused by Erysipelothrix rhusiopathiae, Streptococcus suis, porcine circovirus, and others. Non-infectious and infectious mortality causes both need focus to improve wean-to-finish survival. Because of their importance in affecting survival rate, producers should focus on increasing birth and weaning weight, colostrum intake, and weaning age. Strategies to reduce abdominal organ torsions and gastric ulcers also are important for producers experiencing problems with these low frequency, high magnitude problems. Elevating biosecurity to prevent introduction of pathogens can reduce mortality due to infectious agents. For pathogens already present in the herd, producers must determine if the best management strategy is control (ex. vaccination), elimination (ex. mass exposure), or depopulation. Reducing post-weaning mortality remains an opportunity area in the swine industry.

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Gabler, Nicholas K. "207 Awardee Talk: The Impact of Disease on Nutrient and Energy Intake and Utilization to Support Lean Tissue Accretion in Growing Pigs". Journal of Animal Science 100, Supplement_3 (21 settembre 2022): 93–94. http://dx.doi.org/10.1093/jas/skac247.183.

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Abstract Optimal lean tissue production can be severely compromised by health challenges in nursery-finisher pig production systems. Common pig immune challenges in the U.S. can come from pathogens such as Porcine Reproductive and Respiratory Syndrome virus, Lawsonia intracellularis, Mycoplasma spp, Coccidia, pathogenic E. coli and Porcine Epidemic Diarrhea virus. The subsequent disease that can manifests in response to these pathogens can lead to mitochondrial damage, impaired capacity to generate sufficient ATP for homeostasis, limited energy availability (exacerbated by disease hypophagia) and compromised cell survival and organ function. Collectively, skeletal muscle hypertrophy is reduced in a trade off to support the energetic and protein synthesis needs of immune system response. In growing pigs, we have reported that sub-clinical and clinical enteric and respiratory disease can significantly impact intestinal function, feed intake, lean tissue accretion and feed efficiency. This has a major economic impact for pork producers, pig welfare and wellbeing. While significant advances in molecular and quantitative genetics, clinical diagnostics and virology have been made to enhance poor performance and heath, we are still unsure on how to best feed and manage poor-health pigs. By understanding the longitudinal impact pathogens on digestibility, metabolism, protein accretion and feed efficiency, insights into nutritional requirements and management strategies can be developed to improve poor-health pig production. This paper will discuss the molecular and physiological impact of sub-clinical and clinical disease on nutrient and energy intake and utilization to support lean tissue accretion in growing pigs.

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Nathues, H., R. Tegeler e E. große Beilage. "Diagnostik in einem Mastschweinebestand mit Diarrhö". Tierärztliche Praxis Ausgabe G: Großtiere / Nutztiere 35, n. 02 (2007): 133–36. http://dx.doi.org/10.1055/s-0038-1624014.

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Zusammenfassung: Gegenstand und Ziel: In einem Mastbestand trat in einer Gruppe von etwa 200 Läuferschweinen bei 15–20% der Tiere Diarrhö auf. In einer ersten Untersuchung von zwei Analtupfern auf darmpathogene Infektionserreger konnte die Ätiologie nicht geklärt werden. Material und Methoden: Zehn Kotproben wurden mittels einer Multiplex-PCR auf spezifische Genomfragmente von Lawsonia intracellularis, Brachyspira (B.) hyodysenteriae und B. pilosicoli untersucht. Der Gehalt an Salmonella spp. und hämolysierenden Escherichia coli wurde mittels bakteriologischer Standardverfahren geprüft. Ergebnisse: In vier von zehn Kotproben konnte Lawsonia intracellularis nachgewiesen werden. Andere Enteritiserreger wurden nicht detektiert. Schlussfolgerung: Die Diarrhö im Mastbestand konnte auf eine Infektion mit Lawsonia intracellularis zurückgeführt werden. Klinische Relevanz: Zum Nachweis einer Infektionskrankheit in Herden muss stets eine ausreichende Anzahl Tiere untersucht werden. Für den Nachweis von Lawsonia intracellularis mittels PCR eignen sich Kot- und Schleimhautproben; Analtupfer sind ungeeignet.

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Visscher, Christian, Jasmin Mischok, Saara Sander, Jutta Verspohl, Eva-Ursula Peitzmeier, Isabel von dem Busche e Josef Kamphues. "Spread of an Experimental Salmonella Derby Infection in Antibiotic-Treated or Lawsonia intracellularis Vaccinated Piglets". Animals 8, n. 11 (12 novembre 2018): 206. http://dx.doi.org/10.3390/ani8110206.

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Lawsonia intracellularis infections are a common reason for antibiotic treatment in pig production. Experimental studies in animals naturally infected with Lawsonia intracellularis comparing the course of an experimental Salmonella infection in piglets previously treated with tylosin or vaccinated against Lawsonia intracellularis are scarce. A total of 72 seven-week-old Salmonella-free pigs were taken from a herd with a Lawsonia intracellularis history in piglet rearing. The pigs were divided into two groups with three replicates each. Animals had either been previously treated with tylosin (10 mg/kg body weight) for seven days (AB+VAC−) or had been vaccinated as suckling pigs by drenching (Enterisol®Ileitis; AB−VAC+). Two animals per replicate were primarily infected with Salmonella Derby (1.04 × 108 colony-forming units per animal). The detection of Salmonella in faeces (p < 0.0001, odds ratio: 3.8364) and in the ileocaecal lymph nodes (p = 0.0295, odds ratio: 3.5043) was significantly more frequent in AB+VAC− animals. Overall, the odds ratio for detecting Salmonella in any substrate or organ was significantly higher in the AB+VAC− group animals (p = 0.0004, odds ratio: 5.9091). Treatment with tylosin can significantly increase the spread of a Salmonella infection, which is not observed after early Lawsonia intracellularis vaccination.

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McCluskey, Jackie, Joanne Hannigan, Jennifer D. Harris, Brendan Wren e David G. E. Smith. "LsaA, an Antigen Involved in Cell Attachment and Invasion, Is Expressed by Lawsonia intracellularis during Infection In Vitro and In Vivo". Infection and Immunity 70, n. 6 (giugno 2002): 2899–907. http://dx.doi.org/10.1128/iai.70.6.2899-2907.2002.

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ABSTRACT Lawsonia intracellularis has been identified recently as the etiological agent of proliferative enteropathies, which are characterized by intestinal epithelial hyperplasia and associated moderate immune responses. This disease complex has been reported in a broad range of animals, prevalently in pigs, and L. intracellularis has been linked with ulcerative colitis in humans. L. intracellularis is an obligate intracellular bacterium, and the pathogenic mechanisms used to cause disease are unknown. Using in vitro-grown organisms as a source of genomic DNA, we identified a Lawsonia gene which encodes a surface antigen, LsaA (for Lawsonia surface antigen), associated with attachment to and entry into cells. The deduced amino acid sequence of this protein showed some similarity to members of a novel protein family identified in a number of other bacterial pathogens but for which roles are not fully defined. Transcription of this gene was detected by reverse transcription-PCR in L. intracellularis grown in vitro in IEC18 cells and in bacteria present in ileal tissue from infected animals. Immunohistochemistry with specific monoclonal antibody and immunoblotting with sera from infected animals demonstrated that LsaA protein is synthesized by L. intracellularis during infection. Expression of this gene during infection in vitro and in vivo suggests that this surface antigen is involved during infection, and phenotypic analysis indicated a role during L. intracellularis attachment to and entry into intestinal epithelial cells

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Page, Allen E., Nathan M. Slovis e David W. Horohov. "Lawsonia intracellularis and Equine Proliferative Enteropathy". Veterinary Clinics of North America: Equine Practice 30, n. 3 (dicembre 2014): 641–58. http://dx.doi.org/10.1016/j.cveq.2014.08.001.

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C Rodríguez-Buenfil, Jorge, Mario J Alvarez-Fleites e Rafael M Gómez-Medina. "Identificación de Lawsonia intracellularis en 20 granjas porcinas del estado de Yucatán". REVISTA BIOMÉDICA 11, n. 4 (1 ottobre 2000): 271–75. http://dx.doi.org/10.32776/revbiomed.v11i4.244.

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Introducción. La Enteropatía Proliferativa Porcina es una enfermedad emergente a nivel mundial, causada por una bacteria intracelular recientemente denominada Lawsonia intracellularis. Esta bacteria afecta a todos los cerdos observandose una mayor frecuencia en la población de engorda. En Yucatán no existe información sobre la presencia y distribución de esta enfermedad. Objetivo. Detectar la presencia de Lawsonia intracellularis en cerdos de engorda de granjas porcícolas del estado de Yucatán. Material y Métodos. Se trabajó un total de 20 granjas porcícolas las cuales fueron seleccionadas por conveniencia. Diez unidades de muestreo fueron seleccionadas de cada granja buscando la representatividad y considerando una prevalencia del 25% un nivel de confianza de 95% y una población promedio de 2000 animales. Cada unidad fue conformada recolectando heces de 4 animales de entre 30 y 90 Kg. La técnica diagnóstica que se utilizó en el laboratorio fue la de reacción en cadena de la polimerasa. Resultados. De un total de 20 granjas estudiadas 8 resultaron positivas a la presencia de Lawsonia intracellularis en la población de engorda. Esto representa un 40% de granjas infectadas con prevalencias ≥ al 25%. Con respecto al total de unidades de muestreo 23 (13%) resultaron positivas y 174 (87%) resultaron negativas. Se pudo observar una mayor frecuencia de granjas positivas con poblaciones de engorda mayor a 7000 cerdos de engorda. Conclusión. Se puede concluir que la bacteria Lawsonia intracellularis está presente en granjas porcinas del estado de Yucatán con prevalencias ≥ 25%.

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Riris, Ida Duma, Albinus Silalahi, Tita Juwitaningsih e Wesly Hutabarat. "Antimicrobial Activities of Some Indonesian Medicinal Plants against Propionibacterium acnes (ATCC 27853) and Staphylococcus epidermis (ATCC 12228) causing Acne". Asian Journal of Chemistry 32, n. 1 (18 novembre 2019): 41–44. http://dx.doi.org/10.14233/ajchem.2020.22225.

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Secondary metabolites investigation of local plants, such as Psidium guajava L, Curcuma longa rhizome, Citrus hystrix and Lawsonia intracellularis shown that they have antibacterial activities against Propionibacterium acnes (ATCC 27853) and Staphylococcus epidermis (ATCC 12228) with chloramphenicol as antibiotic controlled. The phytoscreening method used to find out compounds in the plants and inhibition tests carried out by diffuse test with a concentration variation of 10, 5, 2.5 and 1.25 %. It was found that Lawsonia intracellularis plants has the highest inhibitory growth of 17; 12, 15, 14, 12 mm at 10 % concentration.

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Più fonti

Tesi sul tema "Survival of lawsonia intracellularis"

Collins, Alison Marie. "A Study of the Lawsonia intracellularis-induced porcine proliferative enteropathies". University of Sydney. Veterinary Science, 2001. http://hdl.handle.net/2123/835.

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The porcine proliferative enteropathies (PPE) are a group of diseases ranging from intestinal adenomatosis (PIA), a chronic condition causing reduced growth rates in post weaning pigs, to the often fatal proliferative haemorrhagic enteropathy (PHE), resulting in intestinal haemorrhage. PHE predominantly occurs in older and heavier pigs than the chronic disease PIA. This thesis examined whether the age when susceptible pigs are infected affects the clinical response to L.intracellularis infection. The characteristic pathologic lesion of PPE is the abnormal proliferation of crypt epithelial cells in the ileum and colon. Closely associated with this proliferation is the presence of an obligately intracellular bacterium, Lawsonia intracellularis. Characterisation of L.intracellularis was performed in in-vitro co-cultures of L.intracellularis extracted from PHE-affected mucosa. The efficacy of antimicrobials to inhibit the growth of L.intracellularis in-vitro was evaluated and compared with isolates cultured in the United Kingdom. The results were analysed with respect to medication strategies currently used to control PPE in piggeries. PPE occurs in virtually all piggery management systems, including newly developed systems that are aimed at improving the herd health, such as segregated early weaning and multiple site production. PPE is currently controlled in Australia with the routine addition of antimicrobials in pig feed, in particular olaquindox. Recommendations to reduce the use of feed-based antibiotics in Australia require the development of alternate strategies to control diseases such as PPE. Sequential outbreaks of PHE reported in minimal disease herds suggested that pigs could develop immunity to disease. An experimental model of L.intracellularis infection was developed in this thesis to demonstrate that immunity to re-infection with L.intracellularis could be developed. Infection was monitored by detection of faecal shedding of L.intracellularis and serum IgG antibodies against L.intracellularis. Two in-feed antimicrobial strategies were analysed in this thesis for their ability to induce the development of immunity to L.intracellularis, while avoiding clinical signs of disease. The first strategy evaluated the use of low levels of in-feed antimicrobials to allow subclinical infection and the development of immunity. The second strategy evaluated the use of high levels of in-feed antimicrobials to terminate infection two weeks after exposure to L.intracellularis. Gaining a greater understanding of how L.intracellularis infection is spread both within and between piggeries will enable the development of management strategies to control the spread of infection. This thesis examined the possibility that other species in contact with pigs and piggeries such as rats, mice and birds may transmit infection to pigs. The transmission of infection between pigs via the faecal/oral route was also examined, as was the survival and infectivity of L.intracellularis over time. Ultimately this thesis aimed to understand the pattern of L.intracellularis infection and the survival and transmission of L.intracellularis in order to develop effective control measures for PPE, especially in minimal disease herds.

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Collins, Alison Marie. "A Study of the Lawsonia intracellularis-induced porcine proliferative enteropathies". Thesis, The University of Sydney, 2000. http://hdl.handle.net/2123/835.

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Gomes, Nogueira Mariana. "Immunity of lawsonia intracellularis vaccination in pigs". Thesis, The University of Sydney, 2013. http://hdl.handle.net/2123/10421.

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ABSTRACT – Mariana Gomes Nogueira Lawsonia intracellularis is the causative agent of proliferative enteropathy (PE). PE is an important disease of weaner and grower pigs causing degrees of diarrhoea and negative effects on feed intake and weight gain. In-feed antibiotics are routinely used to control PE disease outbreaks. However, increasing restrictions to antibiotic use is being implemented in various pig producing countries. Therefore, alternatives to improve resistance while promoting growth performance are ideal. To limit the establishment of infection and increase profitability of pig production, disease prevention needs both nutritional and immunological strategies and well as effective sanitary measures. Since 2006, in the Australian market, a live attenuated Lawsonia intracellularis vaccine has been used to reduce clinical signs and PE lesions and reduce L. intracellularis shedding in faeces. However, the systemic and local immunological responses to a standard vaccine dose are poorly characterised. In the absence of proof that vaccinated pigs are protected from PE, veterinarians are unwilling to remove antibiotic medication for fear of acute haemorrhagic PE in adult pigs. Additionally, feeding strategies as alternatives to antibiotics, such as beta-glucan have been proposed as a possible option, but no study has investigated the effect of phytase on immune responses in growing pigs. The research in this thesis addresses studies to immune-based investigation of factors affecting the induction of immune responses following vaccination with L. intracellularis. Results revealed that the use of an oral standard and ten times dose of L. intracellularis vaccine protects pigs against PE disease by reducing lesions, shedding in faeces and clinical signs. However, intramuscular delivery also protected pigs against PE. Immunological responses to L. intracellularis vaccination, particularly IgG and cytokines response were observed after oral, intraperitoneal and intramuscular L. intracellularis vaccination. However these were highly variable, highlighting the difficulties in finding suitable biomarkers. The effect of adding S. cerevisae yeast beta-glucan and microbial phytase to weaner diet affected on mucosal and systemic L. intra

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Vu, Ngoc Quynh. "Studies on Lawsonia intracellularis infection in pigs". Thesis, The University of Sydney, 2004. https://hdl.handle.net/2123/28098.

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The porcine proliferative enteropathies (PPE) include a variety of pathological conditions commonly described as separate entities: these include proliferative haemorrhagic enteropathy (PHE), necrotic enteritis (NE), regional ileitis (RI) and porcine intestinal adenomatosis (PIA). All of these pathological conditions are characterised by development of proliferation of the intestinal mucosa, particularly in the distal small intestine and proximal large intestine and the presence of curved, rod-shaped, intracellular bacteria, Lawsonia intracellularz‘s, in the apical cytoplasm of the epithelial cells. PPE can occur in all swine production systems and involve pigs of all age groups, however the more severe clinical from, PHE that causes higher mortality, predominantly affects adult pigs. In the endemic herds, pigs can become infected with L. intracellularis at different time points and shed bacteria in their faeces. Although factors that may play a role in the transmission of L. intracellularis infection have not been identified, spreading of the infection between pigs through the ingestion of contaminated faeces is considered the most important mode of transmission. Using an oral challenge model, this thesis examined how the number of ingested L. intracellularz's could affect the establishment, temporal pattern of infection, clinical disease and immune response to the infection in pigs. Susceptible pigs of all ages exposed to L. intracellularis develop infection and an immune response. They shed L. intracellularis in their faeces and, as the bacterium can not be grown in any artificial medium, the recent development of polymerase chain reaction (PCR) allows the detection of a current infection with L. intracellularis in live pigs by detecting L. intracellularis DNA in the contaminated faeces. Development of immune response to L. intracellularis in infected pigs by production of specific immunoglobulins including IgG, IgM, and IgA in the serum can be detected by the indirect immunofluorescent antibody test (IFAT). One of the aims of this thesis was to investigate the extent of the immunity to L. intracellularis in protecting previously exposed animals from re-infection. In—feed administration of antibiotics is widely practiced in pig production to control many bacterial infections including L. intracellularis as well as for growth promotion. It is important to assess the effect of different groups of antibiotics at different concentrations on the disease pattern and on the development of immunity to re-infection in pigs. Optimising the selection and concentration of some antibacterials can help to reduce the amount and extent of drug use while maintaining a good health status of a pig herd and improving profitability of pig production. As a part of this thesis, an investigation was conducted to determine if L. intracellularis existed in the system of commercial pig farms in the North of Vietnam. Some piggery management factors which may be associated with the occurrence of L. intracellularis infection in these pig herds were also identified and analyzed. The laboratory techniques developed in Australia such as faecal PCR and IFAT appeared to work well and to be useful as tools for diagnosis and survey of L. intracellularis under Vietnamese conditions.

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Nathues, Heiko. "Untersuchungen zur Ausscheidung von Lawsonia intracellularis mittels molekularbiologischer Untersuchungsverfahren". [S.l.] : [s.n.], 2007. http://deposit.ddb.de/cgi-bin/dokserv?idn=985342978.

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Mitchell, Suzanne Christina. "Immune responses in a mouse model of Lawsonia intracellularis infection". Thesis, University of Edinburgh, 2002. http://hdl.handle.net/1842/29892.

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The aim of this thesis was to establish an infection system in the mouse and use this to examine the development of infection and lesions of proliferative enteropathy within the gastrointestinal tract. This mouse model was then used to assess the mucosal and serological responses generated by the host in response to inoculation with L. intracellularis. The infection system demonstrated that 129/Sv/Ev wild-type and isogenic interferon gamma receptor null (IFNγR^-/-) mice developed characteristic lesions of proliferative enteropathy following oral challenge with a pure culture of L. intracellularis (approximately 5xl0⁷ organisms). These were directly comparable to the lesions seen in natural disease with increased proliferation of infected enterocytes in the ileum and colon associated with the loss of goblet cells. Wild-type 129/Sv/Ev mice appeared capable of resolving or resisting infection with this obligate intracellular pathogen, with lesions of proliferative enteropathy detectable only at day 14 or 21 post inoculation, thereafter (days 28 and 35) there were none detectable. In comparison, IFNγR^-/-mice were highly susceptible to disease and appeared unable to eliminate infection over the time course studied, indicating that a functional IFNγ receptor is necessary in the control of infection. This novel system is the first detailed description of proliferative enteropathy in mice and has demonstrated the usefulness of this model in the characterisation and analysis of important immunological events during infection with Lawsonia intracellularis.

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Collins, Alison Marie. "A study of the Lawsonia intracellularis-induced porcine proliferative enteropathies". Connect to full text, 2000. http://hdl.handle.net/2123/835.

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Thesis (Ph. D.)--University of Sydney, 2001.
Includes tables. Title from title screen (viewed Apr. 22, 2008). Submitted in fulfilment of the requirements for the degree of Doctor of Philosophy to the Dept. of Veterinary Clinical Sciences, Faculty of Veterinary Science. Degree awarded 2001; thesis submitted 2000. Includes bibliography. Also available in print form.

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Wenting, Sabine [Verfasser]. "Untersuchungen zur Prävalenz der Lawsonia intracellularis-Infektion bei Absetzferkeln / Sabine Wenting". Hannover : Bibliothek der Tierärztlichen Hochschule Hannover, 2013. http://d-nb.info/1037887735/34.

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Caleffo, Tatiane. "Frequência de Lawsonia intracellularis e rotavírus em equinos criados no oeste do Paraná". reponame:Repositório Institucional da UFPR, 2017. http://hdl.handle.net/1884/47308.

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Orientadora : Profa. Dra. Aline de Marco Viott
Dissertação (mestrado) - Universidade Federal do Paraná, Setor de Ciências Agrárias, Programa de Pós-Graduação em Ciências Veterinárias. Defesa: Palotina, 20/02/2017
Inclui referências : f. 64-73
Resumo: A diarreia é um sinal clínico comum a diversas doenças, sejam de origem bacteriana, viral, ou parasitária. O diagnóstico correto da causa se torna fundamental para o tratamento adequado de potros. A Lawsonia intracellularis é um patógeno muito estudado em suínos, porém nos equinos os primeiros relatos começaram a surgir na década de 1980, e ainda são poucos os casos relatados na literatura veterinária. O rotavírus vem sendo relatado na literatura como causador de diarreias em diversas espécies animais. Em potros, seu relato é atualmente escasso. Foi pesquisada a frequência de L. intracellularis e de rotavírus em equinos com idades entre um mês e cinco anos criados no oeste do estado do Paraná. Os equinos eram provenientes de 15 propriedades oriundos de cinco cidades da região oeste do Paraná. Dos animais utilizados no experimento, 80 (53,69%) eram fêmeas e 69 (46,31%) eram machos. Foram coletadas amostras de sangue e de fezes de 149 animais No exame sorológico de imunoperoxidase em monocamada de células (IPMC), 4,69% dos animais se apresentaram positivos, provenientes de seis propriedades distintas, dentre as 15 pesquisadas. Esses animais apresentavam idades entre seis meses e cinco anos, e títulos sorológicos de 1:60, 1:240 e 1:480. Para a pesquisa de rotavírus foi utilizada a eletroforese em gel de agarose. Não houveram animais positivos para a eliminação de partículas virais nas fezes. Palavras-chave: Enteropatia proliferativa equina; estudo de frequência; diarreia; cavalos.
Abstract: Diarrhea is a common clinical sign of several diseases, of bacterial, viral, or parasitic origin. Correct diagnosis of the cause is essential for proper treatment of foals. Lawsonia intracellularis is a very well know pathogen in swine, however, in foals the first reports dates on 1980, and since then rare cases were report in the veterinary literature. Rotavirus has been describe in the literature as causing diarrhea in several animals. In foals, rotavirus occurrence is currently scarce. The frequency of L. intracellularis and rotavirus was investigated in horses aged between one month and five years old raised in the western state of Paraná. The horses came from 15 farms from the five cities of western Paraná. Of animals used in this research, 80 (53.69%) were females and 69 (46.31%) were males. In serological test using immunoperoxidase performed on cell monolayers (IPMC), 4.69% of animals were positive totalizing six of fifteen farms analyzed. These animals had between six months and five years, and the serological titers varied of 1:60, 1: 240 and 1: 480. For an investigation of rotavirus agarose gel electrophoresis was perform. No one animal was positive for rotavirus in faeces. Key words: Equine proliferative enteropathy; Frequency study; diarrhea; Horses.

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Gomes, Cleise Ribeiro. "Detecção de Lawsonia intracellularis em aves comerciais através da reação em cadeia pela polimerase (PCR)". Universidade de São Paulo, 2007. http://www.teses.usp.br/teses/disponiveis/10/10134/tde-25052007-130622/.

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Abstract (sommario):

A Lawsonia intracellularis é uma bactéria intracelular obrigatória que causa Enterite Proliferativa em vários animais, como suínos, cervos, ratos, hamsters, cobaios, coelhos, ovinos, eqüinos, raposas, cães, furões, primatas não humanos, emas e avestruzes. Atualmente não há relatos da ocorrência do agente ou dos sinais clínicos de sua infecção em aves comerciais (Gallus gallus domesticus). O presente estudo reporta a detecção através da reação em cadeia pela polimerase (PCR) de Lawsonia intracellularis em matrizes pesadas de diferentes idades provenientes de quatro linhagens de aves comerciais. Dentre os 100 suabes de fezes colhidos a partir de fragmentos intestinais, 34% foram positivos para a detecção do agente pela PCR. O agente foi encontrado com maior freqüência em aves de 80 semanas de idade apresentando ou não quadro diarréico. Os fragmentos intestinais das aves cujas fezes foram positivas para detecção de Lawsonia intracellularis foram submetidos ao exame histopatológico e na maioria das lesões analisadas foi observada enterite necrótica crônica proliferativa. Apesar das lesões serem sugestivas de infecção por Lawsonia intracellularis pela coloração de Warthin-Starry, a presença do patógeno no interior dos enterócitos ou células glandulares não foi observada. Esses resultados poderiam levar a novas pesquisas sobre a importância da Lawsonia intacellularis na avicultura do Brasil e do mundo.
Lawsonia intracellularis is an obligate intracellular bacterium responsible for proliferative enteritis in many animals, such as swine, deers, rats, hamsters, guinea pigs, rabbits, ovine, horses, foxes, dogs, ferrets, no human primats, emus and ostrichs. Currently there is no reports about the occurrence of this agent or clinical signs of its infection in chickens (Gallus gallus domesticus). The present study reports the detection of Lawsonia intracellularis at different ages of broiler breeder in four avian commercials lineages by Polymerase Chain Reaction (PCR). Of 100 faecal swabs collected from intestinal fragments, 34% were positive for Lawsonia intracellularis detection by PCR. The agent was more frequently found in chickens of 80 weeks of age presenting or not diarrhea. The intestinal fragments which faeces were positive for Lawsonia intracellularis PCR detection were submitted to histopathological exam and in the most analysed lesions were observed proliferative necrotic cronic enteritis. Although the lesions were suggestive of L. intracellularis infection by Warthin-Starry staining, the presence of the pathogen inside of enterocytes or gland cells was not observed. This results could lead to new researches about the mean of Lawsonia intacellularis in broiler industry of Brazil and the world.

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Libri sul tema "Survival of lawsonia intracellularis"

Lawsonia intracellularis. Servet, 2019.

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Capitoli di libri sul tema "Survival of lawsonia intracellularis"

Gebhart, C. J., e R. M. C. Guedes. "Lawsonia Intracellularis". In Pathogenesis of Bacterial Infections in Animals, 503–12. Oxford, UK: Wiley-Blackwell, 2010. http://dx.doi.org/10.1002/9780470958209.ch26.

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Gebhart, Connie J. "Lawsonia intracellularis". In Interpretation of Equine Laboratory Diagnostics, 185–89. Hoboken, NJ, USA: John Wiley & Sons, Inc., 2017. http://dx.doi.org/10.1002/9781118922798.ch33.

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Pusterla, Nicola, Connie J. Gebhart, Jean-Pierre Lavoie e Richard Drolet. "Lawsonia intracellularis". In Equine Infectious Diseases, 316–21. Elsevier, 2014. http://dx.doi.org/10.1016/b978-1-4557-0891-8.00034-8.

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Lavoie, Jean-Pierre, e Richard Drolet. "Lawsonia intracellularis". In Equine Infectious Diseases, 313–16. Elsevier, 2007. http://dx.doi.org/10.1016/b978-1-4160-2406-4.50041-7.

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LAVOIE, J. "Lawsonia intracellularis Proliferative Enteropathy". In Current Therapy in Equine Medicine, 164–66. Elsevier, 2003. http://dx.doi.org/10.1016/b978-0-7216-9540-2.50051-7.

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Frazer, Michele L. "Lawsonia intracellularis Infection and Proliferative Enteropathy". In Robinson's Current Therapy in Equine Medicine, 345–48. Elsevier, 2015. http://dx.doi.org/10.1016/b978-1-4557-4555-5.00079-0.

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Atti di convegni sul tema "Survival of lawsonia intracellularis"

Tomanova, Katarina, e Vladimir Celer. "Selection of recombinant antibodies against Lawsonia intracellularis". In Sixth International Symposium on the Epidemiology and Control of Foodborne Pathogens in Pork. Iowa State University, Digital Press, 2005. http://dx.doi.org/10.31274/safepork-180809-763.

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Nerem, J. "Lawsonia intracellularis vaccination as an alternative to dietary antimicrobial medication in finishing swine". In Eighth International Symposium on the Epidemiology and Control of Foodborne Pathogens in Pork. Iowa State University, Digital Press, 2009. http://dx.doi.org/10.31274/safepork-180809-836.

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Zhang, Min, Huifang Yin, Ailing Dai, Xiao hua e Xiaoyan Yang. "Prokaryotic Expression and Purification of LscW Protein of Lawsonia intracellularis for biomedical engineering". In 2019 IEEE International Conference on Computation, Communication and Engineering (ICCCE). IEEE, 2019. http://dx.doi.org/10.1109/iccce48422.2019.9010773.

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Larsen, I., A. Mellerup, C. Zachariasen, S. Saxmose Nielsen, A. Folkesson, Ø. Angen, L. Christiansen et al. "Effect of variation in oxytetracycline treatment of Lawsonia intracellularis diahrea in nursery pigs on treatment-efficacy and resistance development". In Fourth International Symposium on the Epidemiology and Control of Salmonella and Other Food Borne Pathogens in Pork. Iowa State University, Digital Press, 2015. http://dx.doi.org/10.31274/safepork-180809-262.

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Artuso Ponte, Valeria, Melanie Abley, Bayleygn Z. Molla, Gireesh Rajashekara, Prosper Boyaka e Wondwossen A. Gebreyes. "Activity of Sangrovit® against Lawsonia intracellularis in grower pigs and its impact on gut physiology and host immunity". In Ninth International Conference on the Epidemiology and Control of Biological, Chemical and Physical Hazards in Pigs and Pork. Iowa State University, Digital Press, 2011. http://dx.doi.org/10.31274/safepork-180809-668.

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Leite, Fernando, Connie Gebhart, Randall Singer e Richard Isaacson. "Vaccination against Lawsonia intracellularis decreases shedding of Salmonella enterica serovar typhimurium in co-infected pigs and changes the host gut microbiome". In Safe Pork 2015: Epidemiology and control of hazards in pork production chain. Iowa State University, Digital Press, 2017. http://dx.doi.org/10.31274/safepork-180809-367.

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Rapporti di organizzazioni sul tema "Survival of lawsonia intracellularis"

Magtoto, Ronaldo L., John K. Johnson e Sheela Ramamoorthy. Lawsonia intracellularis ELISA: A New Test at the ISU-VDL. Ames (Iowa): Iowa State University, gennaio 2010. http://dx.doi.org/10.31274/ans_air-180814-645.

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