Letteratura scientifica selezionata sul tema "Transcriptional interference"

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Articoli di riviste sul tema "Transcriptional interference"

1

O’Callaghan, Chris, Da Lin, and Thomas K. Hiron. "Intragenic transcriptional interference regulates the human immune ligand MICA." Journal of Immunology 200, no. 1_Supplement (May 1, 2018): 109.23. http://dx.doi.org/10.4049/jimmunol.200.supp.109.23.

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Abstract Regulation of MICA expression is incompletely understood, but human MICA can be upregulated in cancer cells, virus-infected cells and rapidly proliferating cells. Binding of MICA to the activating NKG2D receptor on cytotoxic immune cells promotes elimination of the cell expressing MICA. We noted that MICA has tandem promoters that drive overlapping forward transcription. We show that the MICA gene contains a conserved upstream promoter that expresses a non coding transcript. Transcription from the upstream promoter represses transcription from the standard downstream MICA promoter in
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Fang, Zhiming, Zhongming Zhao, Valsamma Eapen, and Raymond A. Clarke. "siRNA Mediate RNA Interference Concordant with Early On-Target Transient Transcriptional Interference." Genes 12, no. 8 (August 23, 2021): 1290. http://dx.doi.org/10.3390/genes12081290.

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Exogenous siRNAs are commonly used to regulate endogenous gene expression levels for gene function analysis, genotype–phenotype association studies and for gene therapy. Exogenous siRNAs can target mRNAs within the cytosol as well as nascent RNA transcripts within the nucleus, thus complicating siRNA targeting specificity. To highlight challenges in achieving siRNA target specificity, we targeted an overlapping gene set that we found associated with a familial form of multiple synostosis syndrome type 4 (SYSN4). In the affected family, we found that a previously unknown non-coding gene TOSPEAK
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Ingelbrecht, I., P. Breyne, K. Vancompernolle, A. Jacobs, M. Van Montagu, and A. Depicker. "Transcriptional interference in transgenic plants." Gene 109, no. 2 (December 1991): 239–42. http://dx.doi.org/10.1016/0378-1119(91)90614-h.

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4

SHEARWIN, K., B. CALLEN, and J. EGAN. "Transcriptional interference – a crash course." Trends in Genetics 21, no. 6 (June 2005): 339–45. http://dx.doi.org/10.1016/j.tig.2005.04.009.

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Hu, Xiao, Susan Eszterhas, Nicolas Pallazzi, Eric E. Bouhassira, Jennifer Fields, Osamu Tanabe, Scott A. Gerber та ін. "Transcriptional interference among the murine β-like globin genes". Blood 109, № 5 (31 жовтня 2006): 2210–16. http://dx.doi.org/10.1182/blood-2006-06-029868.

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Abstract Mammalian β-globin loci contain multiple genes that are activated at different developmental stages. Studies have suggested that the transcription of one gene in a locus can influence the expression of the other locus genes. The prevalent model to explain this transcriptional interference is that all potentially active genes compete for locus control region (LCR) activity. To investigate the influence of transcription by the murine embryonic genes on transcription of the other β-like genes, we generated mice with deletions of the promoter regions of Ey and βh1 and measured transcripti
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Palmer, Adam C., J. Barry Egan, and Keith E. Shearwin. "Transcriptional interference by RNA polymerase pausing and dislodgement of transcription factors." Transcription 2, no. 1 (January 2011): 9–14. http://dx.doi.org/10.4161/trns.2.1.13511.

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Ard, Ryan, and Robin C. Allshire. "Transcription-coupled changes to chromatin underpin gene silencing by transcriptional interference." Nucleic Acids Research 44, no. 22 (September 8, 2016): 10619–30. http://dx.doi.org/10.1093/nar/gkw801.

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Chan, H., S. Hartung, and M. Breindl. "Retrovirus-induced interference with collagen I gene expression in Mov13 fibroblasts is maintained in the absence of DNA methylation." Molecular and Cellular Biology 11, no. 1 (January 1991): 47–54. http://dx.doi.org/10.1128/mcb.11.1.47-54.1991.

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We have studied the role of DNA methylation in repression of the murine alpha 1 type I collagen (COL1A1) gene in Mov13 fibroblasts. In Mov13 mice, a retroviral provirus has inserted into the first intron of the COL1A1 gene and blocks its expression at the level of transcriptional initiation. We found that regulatory sequences in the COL1A1 promoter region that are involved in the tissue-specific regulation of the gene are unmethylated in collagen-expressing wild-type fibroblasts and methylated in Mov13 fibroblasts, confirming and extending earlier observations. To directly assess the role of D
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Chan, H., S. Hartung, and M. Breindl. "Retrovirus-induced interference with collagen I gene expression in Mov13 fibroblasts is maintained in the absence of DNA methylation." Molecular and Cellular Biology 11, no. 1 (January 1991): 47–54. http://dx.doi.org/10.1128/mcb.11.1.47.

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Abstract (sommario):
We have studied the role of DNA methylation in repression of the murine alpha 1 type I collagen (COL1A1) gene in Mov13 fibroblasts. In Mov13 mice, a retroviral provirus has inserted into the first intron of the COL1A1 gene and blocks its expression at the level of transcriptional initiation. We found that regulatory sequences in the COL1A1 promoter region that are involved in the tissue-specific regulation of the gene are unmethylated in collagen-expressing wild-type fibroblasts and methylated in Mov13 fibroblasts, confirming and extending earlier observations. To directly assess the role of D
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Jorgensen, Victoria, Jingxun Chen, Helen Vander Wende, Devon E. Harris, Alicia McCarthy, Shane Breznak, Siu Wah Wong-Deyrup, et al. "Tunable Transcriptional Interference at the Endogenous Alcohol Dehydrogenase Gene Locus in Drosophila melanogaster." G3: Genes|Genomes|Genetics 10, no. 5 (March 25, 2020): 1575–83. http://dx.doi.org/10.1534/g3.119.400937.

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Abstract (sommario):
Neighboring sequences of a gene can influence its expression. In the phenomenon known as transcriptional interference, transcription at one region in the genome can repress transcription at a nearby region in cis. Transcriptional interference occurs at a number of eukaryotic loci, including the alcohol dehydrogenase (Adh) gene in Drosophila melanogaster. Adh is regulated by two promoters, which are distinct in their developmental timing of activation. It has been shown using transgene insertion that when the promoter distal from the Adh start codon is deleted, transcription from the proximal p
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Tesi sul tema "Transcriptional interference"

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Greger, Ingo Harald. "Transcriptional interference between highly expressed eukaryotic genes." Thesis, University of Oxford, 1998. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.298678.

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Watts, Beth Rosina. "Investigating mechanisms of transcriptional interference in Schizosaccharomyces pombe." Thesis, University of Oxford, 2015. https://ora.ox.ac.uk/objects/uuid:c919478f-21e9-4061-81aa-4ec1ae41d223.

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Eukaryotic cells transcribe a vast array of non-coding RNAs, most of which have not been assigned a functional role. The work presented here reveals a novel mechanism of transcriptional repression that is mediated by the non-coding RNA prt (pho1-repressing transcript). The prt transcript is shown to recruit a histone deacetylase, Clr3, to repress pho1. This gene encodes a secreted acid phosphatase essential for phosphate acquisition in fission yeast. In the presence of phosphate, prt is produced from an upstream promoter and leads to silencing of pho1. Thus far, this has been explained by prt
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Chiu, Ya-Lin. "HIV-1 Gene Expression: Transcriptional Regulation and RNA Interference Studies: a Dissertation." eScholarship@UMMS, 2003. https://escholarship.umassmed.edu/gsbs_diss/118.

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Gene expression of human immunodeficiency virus type-1 (HIV-1), which causes Acquired Immunodeficiency Syndrome (AIDS), is regulated at the transcriptional level, where negative factors can block elongation that is overcome by HIV Tat protein and P-TEFb. P-TEFb, a positive elongation transcription factor with two subunits, CDK9 and Cyclin T1 (CycT1), catalyzes Tat-dependent phosphorylation of Ser-5 in the Pol II C-terminal domain (CTD), allowing production of longer mRNAs. Ser-5 phosphorylation enables the CTD to recruit mammalian mRNA capping enzyme (Mce1) and stimulate its guanylyltransferas
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Thomson, Gabrielle Anne Biotechnology &amp Biomolecular Sciences Faculty of Science UNSW. "Retroelements as controlling elements in mammals." Awarded by:University of New South Wales. Biotechnology and Biomolecular Sciences, 2006. http://handle.unsw.edu.au/1959.4/26203.

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Abstract (sommario):
Retroelements are genomic parasites which make up ~42% of the human genome and 38% of the mouse genome. Most are degenerate, but a large number have relatively intact promoter elements, suggesting that they are capable of transcription. Transcriptionally active retroelements can perturb normal transcription units in their vicinity through a variety of mechanisms, leading to phenotypic effects and in some cases disease. This phenomenon of transcriptional interference has been observed in organisms as diverse as maize, Drosophila, and the mouse. We analysed the extent of retroelement transcr
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Puram, Rishi Venkata. "Defining and Targeting Transcriptional Pathways in Leukemia Stem Cells." Thesis, Harvard University, 2014. http://nrs.harvard.edu/urn-3:HUL.InstRepos:13070042.

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Acute myeloid leukemia (AML) is a clonal neoplastic disorder organized as a cellular hierarchy, with the self-renewing leukemia stem cell (LSC) at the apex. Recurrent mutations in transcription factors (TF) and epigenetic regulators suggest that AML is driven by aberrant transcriptional circuits, but these circuits have not been fully defined in an LSC model. To study transcriptional mechanisms relevant to leukemogenesis in vivo, we generated a murine serial transplantation model of MLL-AF9-driven, myelomonocytic leukemia with genetically- and phenotypically-defined LSCs. Using this model,
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Racanelli, Alexandra. "TRANSCRIPTIONAL, EPIGENETIC, AND SIGNAL EVENTS IN ANTIFOLATE THERAPEUTICS." VCU Scholars Compass, 2009. http://scholarscompass.vcu.edu/etd/1876.

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A targeted approach to the development of antifolate therapies has been sought for many years. Central to the success of such development is an understanding of the molecular mechanisms dictating the sensitivity of cells to antifolates and the fundamental differences of these processes between normal and neoplastic phenotypes. This dissertation addressed transcriptional mechanisms and cell-signaling events responsible for the efficacy of antifolate therapies. Transcriptional processes and cell signaling pathways are often aberrant in neoplastic tissues, providing a potential point of distincti
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Mulder, Jaap. "Towards treatment of cholestatic liver disease in children via interference with transcriptional regulation of hepatic transport systems." [S.l. : [Groningen : s.n.] ; University Library Groningen] [Host], 2009. http://irs.ub.rug.nl/ppn/.

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Chery, Alicia. "Rôle de la transcription pervasive antisens chez Saccharomyces cerevisiae dans la régulation de l'expression des gènes." Thesis, Paris 6, 2017. http://www.theses.fr/2017PA066191/document.

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Abstract (sommario):
L'expression des gènes est finement régulée dans la cellule et soumise à de multiples contrôles-qualité. Cette régulation intervient à différents niveaux, de façon à garantir une synthèse efficace des produits fonctionnels de l'expression génique, et pour assurer une adaptation à un changement environnemental. Notamment, les régulations transcriptionnelles sont cruciales pour contrôler la cinétique et le niveau d'expression des gènes. La transcription pervasive est une transcription généralisée non-codante et instable qui fut révélée chez la levure Saccharomyces cerevisiae. Bien que son potent
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Haley, Benjamin. "A Biochemical Dissection of the RNA Interference Pathway in Drosophila melanogaster: A Dissertation." eScholarship@UMMS, 2005. https://escholarship.umassmed.edu/gsbs_diss/9.

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Abstract (sommario):
In diverse eukaryotic organisms, double-stranded RNA (dsRNA) induces robust silencing of cellular RNA cognate to either strand of the input dsRNA; a phenomenon now known as RNA interference (RNAi). Within the RNAi pathway, small, 21 nucleotide (nt) duplexed RNA, dubbed small interfering RNAs (siRNAs), derived from the longer input dsRNA, guide the RNA induced silencing complex (RISC) to destroy its target RNA. Due to its ability to silence virtually any gene, whether endogenous or exogenous, in a variety of model organisms and systems, RNAi has become a valuable laboratory tool, and is even be
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Chery, Alicia. "Rôle de la transcription pervasive antisens chez Saccharomyces cerevisiae dans la régulation de l'expression des gènes." Electronic Thesis or Diss., Paris 6, 2017. http://www.theses.fr/2017PA066191.

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Abstract (sommario):
L'expression des gènes est finement régulée dans la cellule et soumise à de multiples contrôles-qualité. Cette régulation intervient à différents niveaux, de façon à garantir une synthèse efficace des produits fonctionnels de l'expression génique, et pour assurer une adaptation à un changement environnemental. Notamment, les régulations transcriptionnelles sont cruciales pour contrôler la cinétique et le niveau d'expression des gènes. La transcription pervasive est une transcription généralisée non-codante et instable qui fut révélée chez la levure Saccharomyces cerevisiae. Bien que son potent
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Capitoli di libri sul tema "Transcriptional interference"

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Morris, Kevin V. "RNA-Mediated Transcriptional Gene Silencing in Human Cells." In RNA Interference, 211–24. Berlin, Heidelberg: Springer Berlin Heidelberg, 2008. http://dx.doi.org/10.1007/978-3-540-75157-1_10.

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Kjos, Morten. "Transcriptional Knockdown in Pneumococci Using CRISPR Interference." In Methods in Molecular Biology, 89–98. New York, NY: Springer New York, 2019. http://dx.doi.org/10.1007/978-1-4939-9199-0_8.

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Hawkins, John S., Spencer Wong, Jason M. Peters, Ricardo Almeida, and Lei S. Qi. "Targeted Transcriptional Repression in Bacteria Using CRISPR Interference (CRISPRi)." In Methods in Molecular Biology, 349–62. New York, NY: Springer New York, 2015. http://dx.doi.org/10.1007/978-1-4939-2687-9_23.

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Forloni, Matteo, Thuy Ho, Lisha Sun, and Narendra Wajapeyee. "Large-Scale RNA Interference Screening to Identify Transcriptional Regulators of a Tumor Suppressor Gene." In Methods in Molecular Biology, 261–68. New York, NY: Springer New York, 2016. http://dx.doi.org/10.1007/978-1-4939-6518-2_19.

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Ricci, Angela, Silvia Sabbadini, Laura Miozzi, Bruno Mezzetti, and Emanuela Noris. "Host-induced gene silencing and spray-induced gene silencing for crop protection against viruses." In RNAi for plant improvement and protection, 72–85. Wallingford: CABI, 2021. http://dx.doi.org/10.1079/9781789248890.0008.

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Abstract Since the beginning of agriculture, plant virus diseases have been a strong challenge for farming. Following its discovery at the very beginning of the 1990s, the RNA interference (RNAi) mechanism has been widely studied and exploited as an integrative tool to obtain resistance to viruses in several plant species, with high target-sequence specificity. In this chapter, we describe and review the major aspects of host-induced gene silencing (HIGS), as one of the possible plant defence methods, using genetic engineering techniques. In particular, we focus our attention on the use of RNAi-based gene constructs to introduce stable resistance in host plants against viral diseases, by triggering post-transcriptional gene silencing (PTGS). Recently, spray-induced gene silencing (SIGS), consisting of the topical application of small RNA molecules to plants, has been explored as an alternative tool to the stable integration of RNAi-based gene constructs in plants. SIGS has great and innovative potential for crop defence against different plant pathogens and pests and is expected to raise less public and political concern, as it does not alter the genetic structure of the plant.
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Ricci, Angela, Silvia Sabbadini, Laura Miozzi, Bruno Mezzetti, and Emanuela Noris. "Host-induced gene silencing and spray-induced gene silencing for crop protection against viruses." In RNAi for plant improvement and protection, 72–85. Wallingford: CABI, 2021. http://dx.doi.org/10.1079/9781789248890.0072.

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Abstract (sommario):
Abstract Since the beginning of agriculture, plant virus diseases have been a strong challenge for farming. Following its discovery at the very beginning of the 1990s, the RNA interference (RNAi) mechanism has been widely studied and exploited as an integrative tool to obtain resistance to viruses in several plant species, with high target-sequence specificity. In this chapter, we describe and review the major aspects of host-induced gene silencing (HIGS), as one of the possible plant defence methods, using genetic engineering techniques. In particular, we focus our attention on the use of RNAi-based gene constructs to introduce stable resistance in host plants against viral diseases, by triggering post-transcriptional gene silencing (PTGS). Recently, spray-induced gene silencing (SIGS), consisting of the topical application of small RNA molecules to plants, has been explored as an alternative tool to the stable integration of RNAi-based gene constructs in plants. SIGS has great and innovative potential for crop defence against different plant pathogens and pests and is expected to raise less public and political concern, as it does not alter the genetic structure of the plant.
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Misa, Joshua, and Cory Schwartz. "CRISPR Interference and Activation to Modulate Transcription in Yarrowia lipolytica." In Methods in Molecular Biology, 95–109. New York, NY: Springer US, 2021. http://dx.doi.org/10.1007/978-1-0716-1414-3_6.

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Ravelonandro, Michel, and Pascal Briard. "Biogenesis and functional RNAi in fruit trees." In RNAi for plant improvement and protection, 40–46. Wallingford: CABI, 2021. http://dx.doi.org/10.1079/9781789248890.0005.

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Abstract (sommario):
Abstract In plants, genome expression is linked to the transcribed mRNAs that are synthesized by RNA polymerase. Following its move to the cytoplasm, the generated mRNA is briefly translated to the encoded protein. If transcription and translation are dependent on the family of RNA polymerase, these two phenomena could be interfered with through the process designated as gene regulation. Thus, large molecules of RNA (single-stranded or double-stranded) consequently sliced into small molecules produce nascent small interfering RNA ranging from 21 to 27 nucleotides. This chapter revisits the biogenesis of these two types of RNAi, miRNA and siRNA, and notably their involvement in plant gene regulation. Following their sequential transcription and their specific involvement, we will consider the sources and roles of RNA interference in plants and we will look at their detection in fruit crops. We discuss their applications and the risk assessment studies in fruit crops.
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Ravelonandro, Michel, and Pascal Briard. "Biogenesis and functional RNAi in fruit trees." In RNAi for plant improvement and protection, 40–46. Wallingford: CABI, 2021. http://dx.doi.org/10.1079/9781789248890.0040.

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Abstract (sommario):
Abstract In plants, genome expression is linked to the transcribed mRNAs that are synthesized by RNA polymerase. Following its move to the cytoplasm, the generated mRNA is briefly translated to the encoded protein. If transcription and translation are dependent on the family of RNA polymerase, these two phenomena could be interfered with through the process designated as gene regulation. Thus, large molecules of RNA (single-stranded or double-stranded) consequently sliced into small molecules produce nascent small interfering RNA ranging from 21 to 27 nucleotides. This chapter revisits the biogenesis of these two types of RNAi, miRNA and siRNA, and notably their involvement in plant gene regulation. Following their sequential transcription and their specific involvement, we will consider the sources and roles of RNA interference in plants and we will look at their detection in fruit crops. We discuss their applications and the risk assessment studies in fruit crops.
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Hampson, Ian, Gavin Batman, and Thomas Walker. "RNA interference technology." In Tools and Techniques in Biomolecular Science. Oxford University Press, 2013. http://dx.doi.org/10.1093/hesc/9780199695560.003.0006.

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Abstract (sommario):
This chapter explains the RNA interference (RNAi) machinery that regulates post-transcriptional gene silencing. The RNAi technology is considered a vital tool in basic molecular and cellular genetic research, functional genomics, gene expression profiling, drug discovery, prospective disease targeting, and therapies. The chapter describes how the two main classes of small regulatory RNAs-short interfering RNA (siRNA) and microRNA (miRNA)-are generated and how they silence gene expression. It gives an overview of the RNAi pathway and looks at the two main approaches currently used to silence gene expression in vitro , namely, the siRNA technology and the short hairpin RNA (shRNA) technology. Moreover, it discusses the advantages and limitations of the two. The chapter also explores the therapeutic possibilities available using targeted gene silencing.
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Atti di convegni sul tema "Transcriptional interference"

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Shein, M. Yu, G. F. Burkhanova, and I. V. Maksimov. "The effect of bacterial strains on the transcriptional activity of genes of the RNA interference system in wheat (Triticum) infected with Septoria." In 2nd International Scientific Conference "Plants and Microbes: the Future of Biotechnology". PLAMIC2020 Organizing committee, 2020. http://dx.doi.org/10.28983/plamic2020.222.

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In wheat, the level of expression of DCL4 and AGO1 genes correlated with resistance to the pathogen S. nodorum. The transcriptional activity of these genes increased when treated with strains of B. subtilis 26D and B. thuringiensis 11.
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Oakes, Christopher C., Yoon Jung Park, Michael Boutros, and Christoph Plass. "Abstract B6: Revealing DAPK1 transcriptional regulation by RNA interference screening with an integrated BAC‐reporter system." In Abstracts: First AACR International Conference on Frontiers in Basic Cancer Research--Oct 8–11, 2009; Boston MA. American Association for Cancer Research, 2009. http://dx.doi.org/10.1158/0008-5472.fbcr09-b6.

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Zhu, Jingjing, Jeroen Declercq, Karen Willekens, John WM Creemers, Alphons JM Vermorken, and Willem JM Van de Ven. "Abstract 1981: Interference of the polyphenolic compound curcumin with expression regulation of target genes of thePLAG1oncogenic transcription factor." In Proceedings: AACR 103rd Annual Meeting 2012‐‐ Mar 31‐Apr 4, 2012; Chicago, IL. American Association for Cancer Research, 2012. http://dx.doi.org/10.1158/1538-7445.am2012-1981.

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Rapporti di organizzazioni sul tema "Transcriptional interference"

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Dubcovsky, Jorge, Tzion Fahima, Ann Blechl, and Phillip San Miguel. Validation of a candidate gene for increased grain protein content in wheat. United States Department of Agriculture, January 2007. http://dx.doi.org/10.32747/2007.7695857.bard.

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Abstract (sommario):
High Grain Protein Content (GPC) of wheat is important for improved nutritional value and industrial quality. However, selection for this trait is limited by our poor understanding of the genes involved in the accumulation of protein in the grain. A gene with a large effect on GPC was detected on the short arm of chromosome 6B in a Triticum turgidum ssp. dicoccoides accession from Israel (DIC, hereafter). During the previous BARD project we constructed a half-million clones Bacterial Artificial Chromosome (BAC) library of tetraploid wheat including the high GPC allele from DIC and mapped the G
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Tucker, Mark L., Shimon Meir, Amnon Lers, Sonia Philosoph-Hadas, and Cai-Zhong Jiang. Elucidation of signaling pathways that regulate ethylene-induced leaf and flower abscission of agriculturally important plants. United States Department of Agriculture, January 2012. http://dx.doi.org/10.32747/2012.7597929.bard.

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The Problem: Abscission is a highly regulated process, occurring as a natural terminal stage of development, in which various organs are separated from the parent plant. In most plant species, the process is initiated by a decrease in active auxin in the abscission zone (AZ) and an increase in ethylene, and may be accelerated by postharvest or environmental stresses. Another potential key regulator in abscission is IDA (Inflorescence Deficient in Abscission), which was identified as an essential peptide signal for floral organ abscission in Arabidopsis. However, information is still lacking re
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