Segui questo link per vedere altri tipi di pubblicazioni sul tema: Versican.
Tesi sul tema "Versican"
Cita una fonte nei formati APA, MLA, Chicago, Harvard e in molti altri stili
Vedi i top-50 saggi (tesi di laurea o di dottorato) per l'attività di ricerca sul tema "Versican".
Accanto a ogni fonte nell'elenco di riferimenti c'è un pulsante "Aggiungi alla bibliografia". Premilo e genereremo automaticamente la citazione bibliografica dell'opera scelta nello stile citazionale di cui hai bisogno: APA, MLA, Harvard, Chicago, Vancouver ecc.
Puoi anche scaricare il testo completo della pubblicazione scientifica nel formato .pdf e leggere online l'abstract (il sommario) dell'opera se è presente nei metadati.
Vedi le tesi di molte aree scientifiche e compila una bibliografia corretta.
1
Rahmani, Maziar. "Regulation of the versican gene : implications for vascular health and disease". Thesis, University of British Columbia, 2007. http://hdl.handle.net/2429/368.
Testo completoAbstract (sommario):
Versican, a chondroitin sulfate proteoglycan, is one of the main components of the extracellular matrix and hence plays a central role in tissue morphogenesis and a number of pathologic processes. My main goal has been to investigate the mechanisms of versican gene regulation, focusing on the signal transduction pathways, promoter regions, cis-acting elements,and trans- factors. This thesis puts forth new knowledge regarding transcriptional regulation of the human versican gene. In chapter III, I present the cloning of a 752-bp fragment of the human versican promoter (- 634/+118 bp) and nine stepwise 5' deletion fragments in the PGL3-luciferase reporter plasmid. Furthermore, I identify three potential enhancer and two repressor regions in this promoter. I also demonstrate that both cAMP and C/EBPf3 enhanced and repressed versican transcription in HeLa cells and rat aortic smooth muscle cells (SMC),respectively, suggesting that versican transcription is differentially regulated by the respective mediator and transcription factor in epithelial cells and SMC. In chapter IV, I reveal the role ofPI3K/PKB/GSK-30 signaling pathway in regulating versican promoter activity and transcription. Furthermore, I identify that the 0-catenin/TCF-4 transcription factor complex, one of the downstream targets of GSK-3[3, mediates versican promoter activity and transcription. In chapter V, I identify that variations in C-terminal regions of TCF family members determine the irrepressor or enhancer properties on Wnt target genes. Furthermore, I show that curcumin is a strong inhibitor of the P-catenin/TCF-p300 mediated gene expression. In chapter VI, I demonstrate that the androgen receptor trans-activates versican transcription in prostate cancer cells. Furthermore, I show cross-talk between the androgen receptor and 13-catenin in regulating versican transcription in prostate stromal fibroblasts. Overall, this study charts previously uncharacterized promoter elements, transcription factors, and signal transduction pathways involved in regulation of the versican gene.
2
Tani, Hirohiko. "Role of Versican in the Pathogenesis of Peritoneal Endometriosis". Kyoto University, 2017. http://hdl.handle.net/2433/227588.
Testo completo
3
Maurice, Sean Bertram. "Metalloproteinase cleavage of versican at the fibroblast cell surface". Thesis, University of British Columbia, 2009. http://hdl.handle.net/2429/14410.
Testo completoAbstract (sommario):
Versican is a large aggregating proteoglycan expressed in the pericellular matrix of
fibroblast cells. It is highly expressed during development and remodeling. The
regulated synthesis and degradation of versican are associated with physiological
remodeling. Versican is expressed in fibroproliferative lesions of human pulmonary
fibrosis and atherosclerosis. Stromal expression of versican is associated with many
forms of cancer and may be predictive of poor prognosis. Abnormal persistence of the
versican-rich matrix may contribute to fibroproliferative and oncogenic processes. The
process of versican degradation is not understood, but as versican is a pericellular
molecule, physiological degradation likely involves cell surface-associated proteolysis.
As such, the overarching hypothesis for this work is that regulated versican turnover
involves the cell surface-associated metalloproteinases ADAMTS-2, MMP-2 and
MT1-MMP, that are expressed in versican-rich remodeling lesions.
ADAMTS-2 is a procollagen N-propeptidase involved in collagen fibrillogenesis. As
procollagen is synthesized in a versican-rich matrix, it was hypothesized that ADAMTS-
2 might bind and process versican. MMP-2 and MT1-MMP in complex with TIMP-2,
are activated at the cell surface during wound healing, pulmonary fibrosis and cancer.
Versican was purified from human fetal lung fibroblast cultures for in vitro proteolysis
experiments. The purified versican preparation was characterized by electrophoresis,
chromatography, spectrophotometry and mass spectrometry. ADAMTS-2 and versican
localization in normal and fibrotic human lungs were investigated. ADAMTS-2 was
shown to co-purify with versican from human fetal lung fibroblasts. Bovine ADAMTS-2
was purified from fetal calf skin and shown to cleave purified human versican.
The plant lectin concanavalin-A (ConA) induces a matrix degradative phenotype and
is used here to investigate the process of versican degradation relative to apoptotic
iii
events in human fetal lung fibroblast cultures. Con-A induced increased expression of
MMP-2 and MT1-MMP in human fetal lung fibroblasts and a concomitant loss of
versican from the matrix. Microarray analysis was used to investigate expression of
possible versican-degrading enzymes and their inhibitors, expressed in response to
ConA. Recombinant MMP-2 and MT1-MMP were shown to process purified versican
in vitro.
This work expands upon the body of knowledge of versican turnover and should help
in the search for therapeutic avenues to treat fibroproliferative and oncogenic
processes.
4
Fielder, Helen Louise. "Structural and Functional Characterisation of the G1 Domain from Human Versican". Thesis, University of Oxford, 2008. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.491447.
Testo completoAbstract (sommario):
Versican, a chondroitin sulfate proteoglycan, fomis large extracellular link proteinstabilised complexes with hyaluronan (HA), thought to confer structural properties such as elasticity of blood vessels. HA binding is mediated through the N-terminal 01 domain of versican (VOl), which is composed of an Ig domain and two contiguous Link modules. In addition to the Link modules, the Ig domain may be required for HA .binding, perhaps stabilising the overall fold of the 01 domain. There is currently no high-resolution structure available for VOl, or for Type C HA-binding domains (HABO), which are comprised ofa pair of Link modules. Previously, VO I from human versican was expressed in Drosophila S2 cells as two N-glycoforms (dVOI); it was shown to interact with HA and this was unaffected by deglycosylation (Seyfried et aI., 2005b). Initial crystallisation attempts were limited by low expression yields (-0.5 mg/litre) and perhaps glycosylation. Here, VOl has - therefore been expressed a) in E. coli (eVGl) and b) in an unglycosylated form in Drosophila S2 cells in the presence of tunicamycin (tVO I), to generate material more suitable for structural studies. eVG1 was refolded by rapid dilution and, after ion exchange chromatography, 7-9 mg/litre pure protein was obtained. eVGl and tVOl have similar HA-binding properties to dVGl, but intrinsic fluorescence analysis indicated that only eVO1 was correctly folded. Glycosylation may therefore be required for correct folding of VG1 in eukaryotic systems. eVG1 also interacts with the TSO-6 Link module (as previously demonstrated for dVG1 (Kuznetsova et aI., 2006)). These functional studies, in combination with information from 10 NMR, ESI-TOF-MS and dynamic light scattering, indicated that eVGI was suitable for further biophysical/functional studies. eVG1 was screened against -1500 conditions, but an eVGI crystal was not obtained. Lack of success with crystallography may be due to a non-functional fraction of the eVOI preparation. Biotinylated-eVOI was found to be a useful tool for histological staining of HA in tissue sections. eVGI is also being used to raise a monoclonal antibody against the GI domain of versican and to investigate whether eVG1 can promote elastogenesis in vascular smooth muscle cells. Previously it has been hypothesised that VGI interacts with HA every HAlO and that this interaction displays all-or-nothing co-operativity (Seyfried et aI., 2006). By SECMALLS analysis it was found that eVOI 'footprints' a length of polymeric HA which is approximately double the length that was previously thought i.e. HAI8-22. Competition experiments using HA22 oligomers indicate that the interaction is positively co-operative, although further experiments need to be performed to ascertain whether the interaction shows all-or-nothing co-operativity. In sununary, a method of producing high yields of VOI has been developed and this material has facilitated structural, functional, cell biology and histology studies. Further characterisation of HA-VOI complex formation is improving our understanding of extracellular matrix organisation.
5
Foulcer, Simon. "Structural and functional studies on the G1 domain of human versican". Thesis, University of Manchester, 2012. https://www.research.manchester.ac.uk/portal/en/theses/structural-and-functional-studies-on-the-g1-domain-of-human-versican(2670c4da-7050-4280-8a7c-fc02ab3e8bb3).html.
Testo completoAbstract (sommario):
The chondroitin sulphate proteoglycan (CSPG) versican forms complexes with hyaluronan (HA), which are essential in a range of functions including cellular proliferation and migration. Four isoforms of versican result from alternative splicing. Furthermore, biological roles have been identified for the proteolytic cleavage product of versican which contains the N-terminal G1 hyaluronan binding domain. All of these versican forms have different tightly regulated tissue expression profiles. Consequently, impaired regulation is associated with a number of disease pathologies. For example the largest variants (V0/V1) have been shown to be negative indicators of disease outcome in a number of malignant cancers and are a marker of disease progression in atherosclerosis. Interestingly, the smaller versican isoform V3 which lacks CS chains has been demonstrated to have the potential to reverse disease associated phenotypes. The motivation for carrying out the work in this thesis was to try and gain a better understanding of how versican functions on a molecular scale. In this regard, the first aim was to investigate the structure of the hyaluronan binding region of versican using a construct called VG1. The structure of VG1 was analysed in the presence and absence of hyaluronan oligomers. This revealed an insight into the multi-modular structure of the versican hyaluronan binding region and demonstrated that on binding to HA, VG1 under goes a conformational change. Furthermore, the interaction between VG1 and longer lengths of hyaluronan (pHA) was investigated. This demonstrated that when VG1 binds to pHA it is does so with positive cooperativity, packing very close to neighbouring VG1 molecules along a chain of HA. One consequence of this interaction was to reorganise pHA into a helical conformation, an organisation that was confirmed by a number of solution phase techniques. The effect of this reorganisation of pHA by VG1 on HA/CD44 interactions was also assessed. Previously the interaction between CD44 (a cell surface hyaluronan receptor) and long chains of HA (>30 kDa) was shown to be irreversible; however we demonstrate that VG1 can reverse this. Furthermore, a TSG-6 enhanced CD44/interaction was also completely reversed by the addition of VG1. This provides an indication that a functional hierarchy of hyaluronan binding proteins may exist which could have important implications in understanding the function of hyaluronan complexes. Currently, we do not know whether intact versican molecules could interact with HA in the same way as VG1. However, preliminary data suggests that the CS-containing variants (i.e. V0, V1 and V2) would not, whereas V3 and versican fragments could. This work provides an exciting mechanistic insight into the function of versican variants and their breakdown products.
6
Kimata, Koji, Takayuki Miura, Hisashi Iwata, Tamayuki Shinomura e Yoshihiro Nishida. "Abnormal occurrence of a large chondroitin sulfate proteoglycan, PG-M/versican in osteoarthritic cartilage". Thesis, Elsevier, 1994. http://hdl.handle.net/2237/16722.
Testo completo
7
Pourmalek, Saloumeh. "Versican in the wound healing matrix : cellular interactions and degradation by matrix metalloproteinases". Thesis, University of British Columbia, 2009. http://hdl.handle.net/2429/7098.
Testo completoAbstract (sommario):
In wound healing, versican is a component of the provisional matrix laid down at the site of injury by proliferating myofibroblasts. Versican interacts with a variety of matrix molecules and is believed to interact with the cell surface. The mechanism of interaction of versican with the cell surface, however, is not well documented. Return to normal tissue structure, at late stages of wound healing, involves degradation of versican and concomitant fibroblast apoptosis. Macrophage enzymes are candidates for versican degradation; however, the mechanisms of actions of these enzymes on versican and the rates and cleavage sites are not yet known.
This thesis tests several hypotheses: 1) Versican interacts with cell surface receptors of myofibroblasts and macrophages; 2) Versican influences myofibroblast cell morphology during wound contraction; and 3) Macrophage matrix metalloproteinases degrade versican during wound resolution.
We first attempted to identify macrophage and fibroblast versican-binding cell surface ligands. Using biotinylated constructs of the C-terminal domain of versican as baits, we identified versican and versican fragments as the main ligands for the C-terminal construct. However, we found that most versican could be released from the cell surface by hyaluronidase treatment, and concluded that versican is held at the fibroblast cell surface mainly through its interaction with hyaluronan.
Next, we examined the influence of versican and hyaluronan on the physical properties of a collagenous matrix, and the cells embedded within the matrix, using a novel 3-dimensional collagen/versican/hyaluronan matrix model. We found that fibroblast cells in matrices containing versican express smooth muscle actin and take on a contractile morphology.
Finally, we hypothesized that macrophage metalloproteinases degrade versican. The macrophage matrix metalloproteinases (MMPs), MMP 2, MMP-7, and MMP-12 were chosen as candidate enzymes, which we localized to the resolving phase of wound healing in the human lung. We found that MMP-7 and MMP-12 cleave versican at multiple sites in vitro, whereas MMP-2 cleaves versican at a limited number of sites. These macrophage enzymes may be important in clearing versican in vivo.
A better understanding of the mechanism of versican degradation could enable therapeutic modification of the disease process in fibrosis, cancer, and nervous system regeneration.
8
Carthy, Jonathon Morgan. "Cellular and molecular biology of Wnt signaling and versican expression in myofibroblast differentiation". Thesis, University of British Columbia, 2011. http://hdl.handle.net/2429/39838.
Testo completoAbstract (sommario):
Wound healing is a complex and dynamic process that restores tissue integrity after injury, but also contributes pathologically to the development of fibrosis. Growing evidence suggests a role for Wnt signaling during normal and aberrant wound healing. The proteoglycan versican is a target of Wnt signaling that is expressed following injury and accumulates pathologically in many chronic inflammatory conditions. In this dissertation, I hypothesized that Wnt signaling and its target versican are key regulators of mesenchymal cell phenotype. In Aim 1, I demonstrated that treatment of cultured fibroblasts with Wnt3a, a canonical Wnt ligand, stimulates the formation of a myofibroblast-like phenotype characterized by increased expression of smooth muscle α-actin. These changes appear to be mediated by Wnt3a upregulating the expression of TGF-β and its associated signaling through SMAD2 in a β-catenin-dependent mechanism. In Aim 2, I show that Wnt3a alters the phenotype of vascular smooth muscle cells and stimulates the formation of a contractile and secretory phenotype in these cells that is associated with increased gap junction communication. Again, these changes occurred through a mechanism that was dependent on canonical Wnt signaling. In Aim 3, I explored the functional roles of versican by examining its expression following injury to cultures of valve myofibroblasts. My data indicate that versican is secreted as extracellular matrix following injury to valve cells, and suggests a role for the membrane receptor CD44 in organizing this provisional versican matrix. In Aim 4, I delved further into the functional roles of versican by expressing this proteoglycan in murine fibroblasts. In this aim I showed that versican expression promotes myofibroblast differentiation, and these changes appear to be mediated by activation of TGF-β signaling. Lastly, in Aim 5, I explored potential intracellular functions for versican, and provide evidence to suggest versican localizes to the nucleus in mesenchymal cells where it regulates the organization of the mitotic spindle during cell division. Collectively, these data suggest Wnt signaling and versican are key regulators of mesenchymal cell phenotype, and as such, are important mediators of a wound healing response.
9
Sotoodehnejadnematalahi, Fattah. "Studies on regulation of versican gene expression by hypoxia in primary human macrophages". Thesis, University of Leicester, 2011. http://hdl.handle.net/2381/10304.
Testo completoAbstract (sommario):
Hypoxia is a hallmark of many pathological tissues. Macrophages accumulate in hypoxic sites and up-regulate a number of hypoxia-inducible genes. The extracellular matrix glycoprotein versican has recently been identified as one such gene, but the mechanisms responsible for hypoxic induction are not well characterised. Here, hypoxic up-regulation of versican was investigated in primary human monocyte-derived macrophages. Flow cytometry of isolated peripheral blood mononuclear cells demonstrated a three-fold increase in versican protein in macrophages after 5 days incubation in hypoxia. Subset analysis showed that macrophages, and not lymphocytes, are the main peripheral blood mononuclear cells which express, and show hypoxic up-regulation of, versican protein and mRNA. This study showed that versican mRNA is up-regulated 34-fold after exposure of primary human macrophages to hypoxia for 18hrs. Further investigation showed that versican mRNA decay rates are not affected by hypoxia, indicating that hypoxic induction of versican mRNA is mediated by increased promoter activity rather than increased mRNA stability. Extensive deletion and transfection analysis of proximal versican promoter luciferase reporter constructs identified two regions which are required for high level activity of the promoter in hypoxic primary human macrophages. A recent publication suggested that hypoxic induction of versican mRNA in macrophages is mediated by the hypoxia inducible transcription factor HIF-1α. Here, bacterial lipopolysaccharide and the hypoxia mimetic agents desferrioxamine and cobalt chloride, three stimuli which are known to induce HIF-1α, were used to investigate the role of HIF-1 in the up-regulation of versican mRNA. Neither LPS nor cobalt chloride caused up-regulation of versican mRNA, although control HIF-1 regulated genes were up-regulated, suggesting that high-level transcription of the versican promoter in hypoxia occurs via a HIF-1 independent mechanism. Lastly, two specific inhibitors of PI3-kinase, LY294002 and Wortmannin, were shown to down-regulate hypoxic induction of versican mRNA, suggesting a possible role for PI3-kinase.
10
Miyazaki, Yumiko. "Versican V1 in human endometrial epithelial cells promotes BeWo spheroid adhesion in vitro". Kyoto University, 2019. http://hdl.handle.net/2433/242394.
Testo completo
11
Buhler, Rogerio Borghi. "Distribuição do colágeno tipo I, colágeno tipo III e versican na lâmina própria da prega vocal humana de fetos e adultos: método histoquímico e imunoistoquímico". Universidade de São Paulo, 2008. http://www.teses.usp.br/teses/disponiveis/5/5143/tde-12012009-163655/.
Testo completoAbstract (sommario):
A matriz extracelular apresenta importante papel na fisiologia da fonação sendo necessário o conhecimento de seus componentes. Poucos estudos sobre os componentes da matriz em fetos e a ausência de relatos do versican nesta faixa etária, bem como a necessidade de aprofundar os conhecimentos sobre os componentes da matriz em adultos, resultaram na elaboração deste estudo. Analisar a presença e distribuição do colágeno tipo I, colágeno tipo III e proteoglicano versican na lâmina própria da prega vocal de laringes fetais e adultas é o objetivo do estudo. No grupo fetal foram estudadas 7 laringes obtidas de cadáveres variando de 28 a 36 semanas de idade gestacional. No grupo adulto foram estudadas 20 laringes, 10 do sexo masculino, com média de idade de 66 anos, e 10 do sexo feminino com média de idade de 70 anos, com idades pareadas. Utilizou-se método imunoistoquímico para avaliar a expressão do versican no grupo fetal e adulto. Para a avaliação das fibras colágenas utilizou-se o método da Picrosirius polarização no grupo fetal e imunoistoquímico no grupo adulto. A quantificação das fibras colágenas e do versican foi realizada por meio de análise digital de imagens. Os resultados do grupo fetal mostraram uma distribuição homogênea das fibras colágenas formando uma estrutura monolaminar com um entrelaçamento entre as fibras denominado arranjo em cesta de vime; em relação ao versican, este apresentou uma densidade maior na camada superficial e intermediária da lâmina própria. Os resultados do grupo adulto mostraram uma menor densidade de colágeno tipo I na camada intermediária quando comparado à camada superficial (p<0.001) e camada profunda (p=0.005). O colágeno tipo III apresentou distribuição mais homogênea nas camadas da lâmina própria, com uma densidade estatisticamente menor na camada intermediária quando comparada à camada profunda (p=0.001), mas sem diferença estatística na camada superficial. O versican apresentou densidade menor na camada superficial quando comparado à camada intermediária (p=0.036) e camada profunda (p=0.013). Houve menor densidade de versican nas mulheres quando comparado aos homens, percebida apenas na camada superficial. Quando todas as camadas foram consideradas conjuntamente houve uma correlação positiva entre a densidade de versican e colágeno tipo III (r=0.57; p=0.010). Este estudo mostrou as diferenças na distribuição dos componentes da matriz nas camadas da lâmina própria em dois grupos etários proporcionando uma discussão sobre as implicações destes achados na fisiologia vocal e sugerindo que as estruturas glóticas já estariam preparadas para a vocalização ao nascimentoExtracellular matrix has an important rule in the vocal fold physiology and the knowledge about your components is necessary. Fewer studies about matrix extracellular and no studies about versican in fetuses, and the necessity to improve the knowledge about extracellular matrix components in adults group came us to the elaboration of this study that has the objective to analyze the presence and distribution of collagen type I, type III and versican in human vocal fold lamina propria of fetal and adult larynges. Seven fetal larynges obtained from 28- to 36- week-old cadaveric fetuses were studied. Twenty larynges were obtained from adults (10 males and 10 females). Mean age of males was 66 and 70 from females, without significant statistical difference between groups. For the analysis of versican expression, immunohistochemical reactions were carried out in fetal and adult group. The larynges were analyzed through Picrosirius polarization method and immunohistochemistry to visualize the collagen fibers, in fetal and adult group respectively. Collagen fibers and proteoglycan were quantified using a digital image analysis system. In fetuses, the collagen fibers system exhibited homogeneous distribution pattern in a monolaminar layer and spatial arrangement as in a wicker basket; predominance of versican distribution was found out on the superficial and intermediate layer of vocal fold lamina propria. In adult group, there was a lower collagen type I density in the intermediate layer when compared to the superficial (p<0.001) and the deep layers (p=0.005). Collagen type III presented a lower density in the intermediate layer when compared to the deep layer (p=0.001) but without differences in the superficial layer. There was a lower versican density in the superficial layer when compared to the intermediate layer (p=0.036) and deep layer (p=0.013). There was a lower versican density in the lamina propria of females when compared with males. The difference was noted in the superficial layer only. When all layers are considered together there was a positive correlation between versican and collagen type III densities (r=0.57; p=0.010). This study shows the differences in the extracellular matrix components distribution in lamina propria vocal folds in fetal and adult group permitting a discussion about the implications in vocal fold physiology and suggesting that laryngeal structures will be prepared to vocalization at birth
12
Suwiwat, Supaporn. "Versican : regulation, purification, and biological properties of a candidate prognostic indicator for breast cancer /". Title page, table of contents and summary only, 2003. http://web4.library.adelaide.edu.au/theses/09PH/09phs9679.pdf.
Testo completo
13
Bogen, Oliver. "Identifizierung einer Versican-V2-basierten Splicevariante als IB4-bindenden Oberflächenmarker nozizeptiver, nicht-peptiderger C-Faserneurone". [S.l.] : [s.n.], 2005. http://www.diss.fu-berlin.de/2006/263/index.html.
Testo completo
14
Diamantopoulou, Elvira. "The role of versican in the development of the inner ear and in cancer progression". Thesis, University of Sheffield, 2018. http://etheses.whiterose.ac.uk/22506/.
Testo completo
15
Onken, Julia Sophie [Verfasser], e Peter [Akademischer Betreuer] Hau. "The role of the proteoglycan versican in high-grade gliomas / Julia Sophie Onken. Betreuer: Peter Hau". Regensburg : Universitätsbibliothek Regensburg, 2013. http://d-nb.info/1033216631/34.
Testo completo
16
Ruppert, Anne-Marie. "Rôle des calpaïnes extracellulaires dans la progression des adénocarcinomes lépidiques". Thesis, Paris 6, 2015. http://www.theses.fr/2015PA066317/document.
Testo completoAbstract (sommario):
La calpaïne 1 est une protéase à cystéine activée par le calcium, qui peut être partiellement externalisée. Les calpaines extracellulaires favorisent la résolution de l'inflammation et la réparation des tissus, à travers la prolifération et la migration cellulaire. Le récepteur Toll like (TLR) 2 a été identifié comme une cible des calpaïnes extracellulaires dans les lymphocytes. L'objectif est d'étudier le rôle de la calpaïne extracellulaire 1 dans la progression tumorale de l'adénocarcinome pulmonaire lepidique (ADL). La calpaïne extracellulaire, le fragment soluble de TLR2, le versican et les cytokines étaient analysés par ELISA dans des surnageants de lavage bronchoalvéolaire (LBA) de patients atteints d'ADL (n = 68). La source de calpaïne était analysée par immunohistochimie. TLR2, cible de la calpaïne extracellulaire était étudiée par cytométrie de flux sur les polynucléaires neutrophiles (PNN) et des lignées humaines de cancer bronchiques. Calpain 1 extracellulaire, sécrété par les cellules tumorales, était associée à la progression tumorale, l'inflammation à neutrophiles, avec un facteur de mauvais pronostic de survie (p = 0,003). TLR2 était exprimé sur les cellules tumorales ou les PNN avec une diminution d¿expression après traitement par calpaïne. Le fragment soluble de TLR2 était corrélée à la concentration extracellulaire de calpaïne 1 dans les surnageants de LBA (r = 0,624; p <0,001). Le fragment soluble de TLR2 élevé était associé à la progression tumorale et un environnement pro-inflammatoire La calpain extracellulaire sécrétée par la cellule tumorale, favorise un microenvironnement inflammatoire et la progression tumorale médiée par TLR2 dans ADLCalpain 1 is pro inflammatory calcium-activated cysteine proteases, which can be partly externalized. Extracellular calpains limit inflammatory processes and promote tissue repair, through cell proliferation and migration. Toll like receptor (TLR) 2 has been identified as a target of extracellular calpains in lymphocytes. The aim was to investigate the role of extracellular calpain 1 in tumor progression of lepidic pulmonary adenocarcinoma (LPA). Extracellular calpain 1, soluble fragment of TLR2 and cytokines were analyzed by ELISA in bronchoalveolar lavage fluid (BALF) supernatants from patients with LPA (n=68). Source of calpain was analyzed by immunohistochemistry. TLR2 as target of extracellular calpain was studied by flow cytometry on polymorphonuclear neutrophils (PMN) and human lung cancer cell lines. Extracellular Calpain 1, secreted by tumor cells, was associated to tumor progression, neutrophilic inflammation, with a poor prognostic factor on survival (p=0,003). TLR2 was expressed on PMN or tumor cells and decreased after calpain treatment. The soluble fragment of TLR2 was correlated to the extracellular calpain 1 concentration in the BALF supernatants (r=0.624; p<0.001). High soluble fragment of TLR2 was associated with tumor progression and a pro-inflammatory environment. Extracellular Calpain 1 secreted by tumor cell, promotes inflammatory microenvironment and tumor progression through TLR2 in LPA
17
Damasceno, Karine Araujo. "Carcinomas em tumores mistos mamários caninos: expressão de versican e sua relação com invasão do estroma e com grau de diferenciação mioepitelial". Universidade Federal de Minas Gerais, 2012. http://hdl.handle.net/1843/BUOS-9DUHDR.
Testo completoAbstract (sommario):
Components of the extracellular matrix have been studied with the attempt to elucidate mechanisms involved in the biological behavior of tumors. Among them, the proteoglycan versican deserves highlight. Its presence has been strongly associated to cancer development, cellular differentiation and progression. Carcinomas in benign mixed tumors (CBMT) are the most common malignant tumor in female dogs and may serve as a model for studies on tumor progression. The aim of this work was to evaluate the expression of versican in in situ and invasive carcinomatous areas of CBMT, verifying possible associations with other classic prognostic factors and overall survival. Furthermore, to evaluate the expression of versican, and the relation of versican with the expression of p63, smooth muscle -actin (-SMA), proteoglycan sulfate and mucopolysaccharides in myoepithelial cells in their different differentiation stages. Clinical staging, histological grade, Nottingham Prognostic Index (NPI), immunohistochemical staining for versican, E-cadherin, Ki-67 and confirmation of invasion areas with staining for p63 and -SMA were performed on 49 cases of CBMT for the first proposal. Versican, p63 and -SMA (immunohistochemical) and proteoglycan sulfate and mucopolysaccharides (histochemical) expression were analyzed in 39 eligible cases for the second proposal. Evaluation of double staning for p63 and -SMA indicated that myoepithelial cells still found in carcinomas with invasion areas presented weak or loss of staining of at least one marker. Versican immunoreactivity was less intense adjacent to in situ carcinomatous regions when compared to invasive regions in which staining was found as more extensive areas with strong expression (p < 0.0001). Evaluation of myoepithelial cells in benign areas demonstrated significant differences between the expression of versican and -SMA in normal (p < 0.0001), polygonal (p < 0.0001) and stellate-shaped (p = 0.0013) myoepithelial cells, as well as in the myxoid (p < 0.0001) and immature chondroid (p < 0.0001) matrix. Furthermore, differences were also observed between p63 reactivity and versican in spindle-shaped myoepithelial cells (p = 0.0029), myxoid (p = 0.0174) and immature chondroid areas (p = 0.0156). These results suggest that, as the myoepithelium gains mesenchymal characteristics, a decrease in p63 and -SMA molecule expression and increase in versican expression occurs. In addition direct relation between versican and invasion suggests the role of this molecule in tumor progression.Componentes da matriz extracelular tem sido objeto de estudos na tentativa de elucidar os mecanismos envolvidos no comportamento biológico das neoplasias. Dentre eles, o proteoglicano versican tem merecido destaque. Sua presença tem sido fortemente associada a diferenciação celular, a progressão tumoral, invasão, recidiva e fatores prognósticos. Em cadelas, estudos apontam que os carcinomas em tumores mistos benignos (CBMT), tipo histológico mais frequente nesta espécie, podem servir como modelo para estudo da progressão tumoral. Sendo assim, esse trabalho teve como objetivo avaliar a expressão do proteoglicano versican nas áreas carcinomatosas in situ e nas áreas invasoras neste tipo histológico, verificando sua associação com fatores prognósticos clássicos em fêmeas caninas e sobrevida. Além disso, avaliar a expressão de versican e sua relação com a expressão de p63, -actina de músculo liso (-SMA), proteoglicano sulfatado e mucopolissacarídeos nas células mioepiteliais, nos seus diversos estágios de diferenciação. Para a primeira proposta foram estudados 49 casos de CBMT nos quais foram feitos estadiamento clínico, a partir de dados obtidos nas fichas clínicas, grau histológico, Índice de Prognóstico de Nottingham (IPN), identificação imuno-histoquímica de versican, p63 e -SMA para confirmar as áreas de invasão, além de E-caderina e Ki-67. Para a segunda proposta foram elegíveis 39 casos, nos quais foram analisadas as expressões de versican, p63 e -SMA (imuno-histoquímica), de proteoglicano sulfatado e mucopolissacarídes (histoquímica). A imunorreatividade para versican revelou-se menos intensa adjacente às regiões carcinomatosas in situ quando comparado às de invasão, onde foram observadas áreas mais extensas de forte expressão (p < 0.0001). Na avaliação das células mioepiteliais nas áreas benignas foram observadas diferenças significativas entre a expressão de versican e -SMA nas células normais (p < 0.0001), poligonais (p < 0.0001), estreladas (p = 0.0013), assim como na matriz mixóide (p < 0.0001) e pré-condróide (p < 0.0001). E ainda, diferenças também foram observadas entre a reatividade de p63 e versican pelas células mioepiteliais fusiformes (p = 0.0029), na área mixóide (p = 0.0174) e pré-condróide (p = 0.0156). Estes resultados sugerem que à medida que o mioepitélio adquire características mesenquimais ocorre uma diminuição na expressão das moléculas de p63 e alfa-actina e aumento da expressão da versican. Além disso, a relação direta entre versican e invasividade sugere o papel desta molécula na progressão destes tumores.
18
Broiles, JoSette Leigh Briggs. "The use of a tissue engineered media equivalent in the study of a novel smooth muscle cell phenotype". Diss., Atlanta, Ga. : Georgia Institute of Technology, 2008. http://hdl.handle.net/1853/22652.
Testo completoAbstract (sommario):
Thesis (Ph. D.)--Mechanical Engineering, Georgia Institute of Technology, 2008.Committee Chair: Nerem, Robert; Committee Member: Chaikof, Elliot; Committee Member: Taylor, W. Robert; Committee Member: Vito, Raymond; Committee Member: Wight, Thomas.
19
Pettersson, Oscar. "Lega Version 2". Thesis, Mälardalen University, School of Innovation, Design and Engineering, 2010. http://urn.kb.se/resolve?urn=urn:nbn:se:mdh:diva-9629.
Testo completoAbstract (sommario):
Detta arbete går ut på att uppfinna och utveckla en enhet som integrerar människor med hjälp av teknik och som ska användas på en konsthall. Prototypframtagning och en optimerad designprocess utmynnar i underlag som ska användas för framställning av denna enhet i större skala. Detta har lösts genom brainfire som generade tre olika koncept. Dessa koncept kombinerades även i ett fjärde koncept som antogs som vinnande koncept efter genomgång av PUGHs beslutsmatris. Konceptet optimerades med hjälp av PU-verktygen DFA, DFM samt DFE. Detta ledde till en monteringsoptimerad, ekonomioptimerad och återvinningsbar enhet. Enheten kläms ihop när användaren vill säga något som ska sparas. Det sagda sparas via radio till ett minne på en klisterlapp. Klisterlappen ges sedan vidare till nästa användare som kan klistra på klisterlappen på en enhet. När enheten då passerar den plats där den förste användaren sparade det denne sade så spelar den upp en spökviskning av det den första användaren sa. På så vis ökar enheten kommunikationen mellan användarna vilket är målet med arbetet. Enheten uppfyller samtliga krav i kravspecifikationen och är återvinningsbar, optimerad för låg energiförbrukning, rengörningsbar, separabel samt innehar lång livslängd i förhållande till dess arbete. Industridesignen upplevs av utomstående som tilltalande, tilldragande, innovativ, futuristisk, intressant, karaktärsrik och ergonomisk.
20
Ali, Amjad. "Migration from Internet Protocol Version 4 To Internet Protocol Version 6". Thesis, Linnéuniversitetet, Institutionen för datavetenskap (DV), 2014. http://urn.kb.se/resolve?urn=urn:nbn:se:lnu:diva-38322.
Testo completoAbstract (sommario):
IPv4 has played it big role in spreading Internet and Internet based applications for more than 20 years. Now it will hand over the stage to its more powerful successor IPv6. IP is an important component of the TCP/IP protocol suit and the Internet is built on it. IPv6 is a new generation protocol suite which has been proposed by the Internet Engineering Task Force (IETF) which uses the 128-bit address instead of IPv4 32-bit address. Moving to the next generation of Internet Protocol became an issue to solve many problems in the current generation. Unfortunately IPv4 and IPv6 are incompatible with each other. It is necessary to create smooth transition mechanisms that a transition mechanism is required during the time of migration from IPv4 to IPv6 networks. This paper aims to supplement this by presenting the design and implementation of IPv4 to IPv6 Transition Scenarios. This paper very clearly illustrates the transition of IPv4-to-IPv6 Transition mechanisms along with how to execute IPv6 commands.
21
Tavares, Raquel Aguiar. "Estudo histológico da matriz extracelular do músculo cricofaríngeo em cadáveres de diferentes idades". Universidade de São Paulo, 2009. http://www.teses.usp.br/teses/disponiveis/5/5143/tde-24022010-155855/.
Testo completoAbstract (sommario):
O músculo cricofaríngeo desempenha um importante papel na deglutição. Acredita-se que seu comportamento elástico seja dependente não apenas do componente muscular, mas também do tecido conectivo intramuscular. O objetivo desse estudo foi analisar a presença e a distribuição do colágeno total, colágenos tipo I e III, fibras elásticas, fibronectina e versican no endomísio do músculo cricofaríngeo em cadáveres de diferentes idades. Vinte e sete músculos foram obtidos mediante autópsia de indivíduos de ambos os sexos com idades entre 28 e 92 anos. Foram realizadas colorações histoquímicas, Método Picrossírius e Método Resorcina-fuccina com oxidação prévia pela oxona, e imunoistoquímicas, para colágeno tipo I, tipo III, fibronectina e versican. A medida dos elementos estudados foi feita por meio de um sistema de análise de imagens que incluía um microscópio, conectado a um computador por meio de uma câmera de vídeo. Foi utilizado o software Image pro Plus, versão 4.1. Para cada caso, quinze imagens não sobrepostas de cada coloração no aumento de 400x foram analisadas. A área de marcação positiva dentro do endomísio do músculo cricofaríngeo foi determinada por um padrão de cor específico para cada coloração. A área de cada elemento da matriz extracelular foi expressa como porcentagem da área total do estudada. Os dados foram expressos em medianas e intervalos interquartílicos. A correlação entre idade e os diferentes elementos da matriz extracelular foi realizada por meio da correlação de Spearman. O teste de Mann-Whitney para distribuição não paramétrica foi utilizado para comparar as áreas porcentuais e os indivíduos de diferente sexo. Todos os testes foram realizados pelo software SPPS versão 13.0 e foi admitido um calor de significância com p < 0,05. O colágeno foi o elemento mais abundante dentre os estudados. Encontrou-se fibras elásticas longitudinais à fibra muscular, finas fibras transversais entre as fibras musculares e espessamento as fibras elásticas nos pólos da fibra muscular. Foi encontrada uma grande variação no conteúdo de fibronectina e versican entre os casos. Não foi evidenciada diferença estatisticamente significativa entre os elementos estudados, o sexo e a idade. Os resultados sugerem que a presença e distribuição desses elementos são importantes para a função do músculo cricofaríngeo e para a manutenção da homeostase. A porcentagem de colágeno encontrada é condizente com a característica esfinctérica do músculo e o arranjo de fibras elásticas contribui para o comportamento elástico e a capacidade de rapidamente reassumir a posição tônica após a abertura durante as deglutições. As variações da quantidade de fibronectina e versican, podem ser resultante da susceptibilidade a fatores agressores. A ausência de alterações com a idade pode significar que o músculo não esteja sujeito às mesmas alterações decorrentes da idade que outros músculos esqueléticosThe cricopharyngeus muscle is thought to play an important role in swallowing and related activities. Its elastic behavior is likely to depend not only on its muscular components, but also on the intramuscular connective tissue. Our objective is to analyze the presence and distribution of total collagen, type I and III collagen, elastic fibers, fibronectin and versican in cricopharyngeus muscle endomysium in adults of a wide age range. Twenty-seven cricopharyngeus muscles obtained from male and female cadavers (age range, 28-92 years-old) were analyzed with the Picrosirius method, oxidized Weigert resorcin-fuchisin, immunohistochemistry. Quantification of stained areas in the cricopharyngeus endomysium with different techniques was performed by an image analysis system connected to a light microscope. The correlation between age and the density of different extracellular matrix proteins was tested using Spearman test. T-tests for independent samples were used to analyze the influence of gender and smoking habit on the fractional areas of extracellular matrix. Collagens had the highest density among the analyzed components. Elastic fibers surrounded each muscle cell, longitudinal to their long axis, associated to traversing fibers, forming a fiber network embedding muscle cells. There was a wide variation on fibronectin and versican content among cases. There were no statistical significance for analysis made between those components of extracellular matrix and age andgender. Our findings suggest that presence and distribution of these extracellular matrix components are important to cricopharyngeus muscle homeostasis. The elastic fibers arrangement can contribute for the cricopharyngeus muscle elastic behavior and ability to rapidly reassume its tonic position after opening during swallows. Variations in the expression of fibronectin and versican can beresultant of its injury susceptibility. The absence of changes on extracellular components during aging could mean that cricopharyngeus muscle is not susceptible to similar age changes as other skeletal muscles
22
Karlsson, Nina. "Language Manager Version 2.0". Thesis, Örebro universitet, Institutionen för naturvetenskap och teknik, 2013. http://urn.kb.se/resolve?urn=urn:nbn:se:oru:diva-30043.
Testo completoAbstract (sommario):
This report describes an examination project made for the IT consultingcompany Sogeti. The purpose of the project was to develop and modify the translation tool Language Manager (LM) built by Sogeti to be used for translating applications. Employees at Sogeti considered some disadvantages with Language Manager, version 1.0 which among others was that language files for projects were saved at two locations. Partly in resource maps among with the source code of the applications and partly in a database. This was dual work for employees at Sogeti and it also caused redundancy inthe system. Also employees at Sogeti thought that the managing of projects and versioning did not adapt to how the system was needed to be used. The destination by the examination project was to remove the database and only use XML-files to handle languages, and also to make the new Language Manager easier to work with. New users should easily understand how to handle terms and translation in the new application and no manual should be needed to perform tasks. Language Manager version 2.0 should be written in C# .Net Framework 4.5 and the graphical user interface should be created with Windows Presentation Foundation (WPF). Sogeti wished for the Model-View-ViewModel pattern (MVVM) to be implemented. The new tool was supposed to be robust and simple with a future-safe architecture.Den här rapporten beskriver ett examensarbete som genomfördes åt IT-konsultbolaget Sogeti med syfte till att vidareutveckla och omarbeta översättningsverktyget Language Manager (LM) som var tillverkat av Sogeti och som användes till att översätta applikationer. Anställda på Sogeti ansåg att det fanns vissa nackdelar med Language Manager version 1.0 som bland annat var att språkfiler för projekt lagrades på två platser. Dels i resursmappar tillsammans med applikationernas källkod och dels i en databas. Detta medförde dubbelt arbete för de anställda på Sogeti när de arbetade med Language Manager och det orsakade även redundans i systemet. På Sogeti ansåg man även att hanteringen av projekt och versionshanteringen av språkdata i Language Manager version 1.0 inte passade ihop med hur man arbetade med programmet. Målet med examensarbetet var att avlägsna databasen och endast arbeta med XML-filer som förvaring av språk och att Language Manager version 2.0 skulle bli enklare och mer lättarbetat. Nya användare skullemed lätthet förstå hur hantering av termer och översättning skulle göras utan hjälp av manual. Det nya översättningsverktyget skulle skrivas i C# .Net Framework 4.5 och Windows Presentation Foundation (WPF) skulle användas för att implementera det grafiska gränssnittet tillsammans med Model-View-ViewModel-mönstret (MVVM).Särskild inriktning skulle framför allt vara mot robusthet, enkelhet och med en framtidssäker arkitektur.
23
Tardy, David. "Autour de la version : vers une méthodologie d'enseignement universitaire de la version anglaise". Nancy 2, 1998. http://www.theses.fr/1998NAN21029.
Testo completoAbstract (sommario):
Cette thèse se propose de mettre en avant les problèmes spécifiques lies aux cours de version anglaise à l'université française et d'y remédier, problèmes tels qu'ils sont évoqués par les spécialistes, les enseignants et les étudiants. Ils sont de plusieurs ordres : problèmes lies à la traduction en soi (validité, difficulté), problèmes concrets (difficulté de la matière, de l'examen, correspondance de celui-ci avec les cours), problèmes méthodologiques (mise en place de savoir-faire spécifique), validité de la matière. Après avoir restitué la problématique en établissant brièvement un historique de la traduction, de son enseignement, en insistant sur les particularités de l'anglais, la thèse analyse les avis des spécialistes sur les problèmes posés par la traduction et le cours de version, ceux d'enseignants à partir d'entretiens, puis ceux d'étudiants à partir d'un questionnaire et d'entretiens. En ayant dégagé des points concrets, la thèse envisage des solutions pour améliorer les cours. En conciliant pragmatisme et théorie. Notamment, certains exercices novateurs (traduction en chaine, étude de traductions officielles) ont été testés sur des étudiants. Des particularités ont été dégagées : la traduction serait une activité naturelle en germe chez l'individu et l'enseignant doit développer les savoir-faire nécessaires par un entrainement approprie. Le cours de version ne doit donc pas seulement aider l'étudiant à obtenir son examen ou à améliorer son anglais général mais également à développer ce savoir-faire unique et utile.
24
Pendzich, Marc. "Von der Coverversion zum Hit-Recycling : historische, ökonomische und rechtliche Aspekte eines zentralen Phänomens der Pop- und Rockmusik /". Berlin [u.a.] : LIT, 2008. http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&doc_number=017060616&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA.
Testo completo
25
Hernández, Martínez Daniel. "Efecto del silenciamiento de CD44 y versicano en líneas celulares de melanoma humano". Doctoral thesis, Universitat Autònoma de Barcelona, 2009. http://hdl.handle.net/10803/3616.
Testo completoAbstract (sommario):
El versicano es un proteoglicano de matriz extracelular cuyas isoformas grandes (V0 y V1) han demostrado participar en la regulación de procesos como la proliferación, adhesión y migración celular. Su expresión se ha encontrado en diferentes tipos de células tumorales, entre ellos el melanoma. Anteriormente se describió que la expresión de la isoforma pequeña de versicano (V3) en dos líneas celulares de melanoma humano (una que expresa las isoformas grandes de versicano de forma endógena y otra que no expresa ninguna isoforma) provoca una reversión del fenotipo maligno. En el caso de las células que expresan versicano de forma endógena, este efecto del versicano V3 se explicó por una competencia con dichas isoformas, mientras que en el caso de la línea que no expresa versicano la acción de V3 iría mediada a través de una interacción con el receptor de ácido hialurónico CD44. La finalidad de este trabajo ha sido confirmar estas hipótesis mediante el silenciamiento por RNA de interferencia de la expresión de CD44 y versicano en estas líneas. Los resultados confirman que la mayor parte de los efectos del versicano V3 tienen lugar a través de los mecanismos propuestos.
26
Domenzain, Reyna Clelia. "Regulación de la expresión de versicano y su relación con la diferenciación de las células de melanoma humano". Doctoral thesis, Universitat Autònoma de Barcelona, 2006. http://hdl.handle.net/10803/3558.
Testo completoAbstract (sommario):
La composición de la matriz extracelular (MEC) determina algunos aspectos cruciales del comportamiento tumoral. Entre sus componentes destacan glicoproteínas y proteoglicanos (PGs), algunos de los cuales tienen receptores en la membrana celular. Nuestro grupo de investigación está interesado en la biología de los PGs en el melanoma humano. El VS es un PG de matriz de la familia de los hialectanos. Está formado por tres dominios: G1, G2 y G3. El splicing alternativo del dominio central G2 genera cuatro isoformas denominadas V0, V1, V2 y V3.
En células de melanoma humano se ha descrito la expresión de proteoglicanos como el proteoglicano específico de melanoma (mel-PG), CD44 o versicano (VS), cuya presencia fue descrita en nuestro laboratorio. Asimismo, describimos su capacidad para incrementar la proliferación y migración en células de melanoma humano, además de disminuir su adhesión.
En la primera parte de este trabajo determinamos el patrón de expresión de las isoformas de VS y de mel-PG durante el proceso de desdiferenciación de las células de melanoma, utilizando líneas celulares con diferentes grados de diferenciación (temprano, intermedio y tardío).
Nuestros resultados muestran que el grado de diferenciación de las células determina la expresión diferencial de VS y mel-PG, de tal forma que las líneas celulares más indiferenciadas expresan todas las isoformas de versicano y las más diferenciadas ninguna de ellas. De ello concluimos que la pérdida del estado diferenciado se acompaña de la expresión de las diferentes isoformas de VS, hecho que parece ser común en tumores derivados de células con mismo origen embrionario, como muestran nuestros análisis preliminares realizados en células de neuroblastoma y astrocitoma.
Durante el proceso de diferenciación in vitro de las líneas celulares SK-mel-1.36-1-5 y SK¬mel-3.44 estos PGs desaparecen, por lo que proponemos que la producción de versicano es señal de regresión a un estado no diferenciado durante la progresión tumoral, y que existe un patrón temporal para la expresión de cada isoforma de versicano.
En la segunda parte analizamos la regulación del promotor de VS en líneas celulares de melanoma humano con grados de diferenciación temprano y tardío.
Aislamos, secuenciamos y clonamos el promotor de VS humano. El análisis in silico del mismo determinó tres regiones de regulación: la primera en el extremo 5', presenta dos cajas de reconocimiento para TCF-4; la segunda en la zona central, 6 cajas de unión para Sp1, 7 para AP¬2 y una para Smad3/4; y la tercera en el extremo 3', una caja de unión para AP-1.
Construimos diversos mutantes de deleción diseñados para aislar el papel de cada uno de los elementos reguladores del promotor de VS y los transfectamos de manera transiente en las líneas celulares.
Los resultados muestran que TCF-4 es un activador responsable de más del 50% de la actividad total de este promotor, ya que la sobrexpresión de algunos de los participantes de la vía de señalización de TCF-4 tiene un efecto directo sobre la actividad del mismo. Además, mediante ensayos de EMSA determinamos la unión específica del complejo TCF-4/?-catenina. En la región central reside aproximadamente el 10% de la actividad total del promotor y hemos determinado la unión específica a 3 de los posibles sitios de unión para Sp1. Planteamos por tanto, que Sp1 es un modulador o quizás regulador de respuesta rápida de VS. Por último, en la región 3' del promotor, que contiene la secuencia de reconocimiento para AP-1, se encuentra el 40% restante de la actividad y hemos determinado que dicha secuencia es la responsable de la actividad basal de VS.
Extracellular matrix composition is crucial for some aspects of tumor behavior. Among its constituent factors, glycoproteins and proteoglycans play very important roles (some of which even having cell membrane receptors). Our research group is interested on proteoglycan biology in human melanoma.
Versican is an extracellular matrix proteoglycan. It is a member of the hyalectan family and has three domains called G1, G2 and G3. The G2 central domain undergoes alternative splicing to generate four isoforms known as V0, V1, V2 and V3.
The presence of proteoglycans such as the melanoma specific proteoglycan (mel- PG), CD44 or versican (VS), has been described in human melanoma cells. In our laboratory we have described VS presence in human melanoma cells and have showed how it increases cell migration and proliferation and how it diminishes cellular adhesion.
In this work we first determined the expression pattern for both the four versican isoforms and mel- PG during melanoma cells desdifferentiation process. We used cell lines under early, intermediate and late differentiation degrees.
Our results show that there is a close correlation between cell differentiation and either versican or mel-PG expression, in such a way that undifferentiated cells express all VS isoforms whereas the more differentiated express none. From this we concluded that the loss of a differentiated state is often accompanied by the expression of VS isoforms: our preliminary results using neuroblastoma and astrocytoma cells show that this might be a common fact among tumors originating from cells having the same embryonic origin.
During in vitro differentiation, SK-mel-1.36-1-5 and SK-mel-3.44 cell lines loose these proteoglycans. For this reason we propose that versican production signals the regression to an undifferentiated state during tumor progression, and that there is a temporal pattern for the expression of each versican isoform.
In this work we also analyzed versican promoter regulation in human melanoma cell lines under early and late differentatiation.
We isolated, sequenced and cloned VS human promoter. Its in silico analysis showed that there are three regulation zones: 1) at the 5' end there are two recognition boxes for TCF-4. 2) In the central zone there are six binding boxes for Sp1, 7 for AP-2 and one for Smad3/4. 3) Finally, at the 3' end there is a binding box for AP-1.
We also constructed several deletion mutants designed to study the role of each these regulating elements in the VS promoter. These were transiently transfected into the cell lines.
Our results show that TCF-4 is responsible for more than 50% of the promoter's total activity as seen by the overexpression of some of the participants in the TCF-4 signaling pathway. Furthermore, through EMSA analysis we determined the specific union of the TCF-4/?-cathenine complex. The central region counts for roughly 10% of the promoter's total activity where we have determined the specific union for three of the possible Sp1 binding sites. For this reason, we postulate that Sp1 is a VS fast response regulator. Finally, the 3' region is responsible for the remaining 40% of promoter activity and we have determined that it is responsible for versican's basal activity.
27
Eriksson, Agnes. "Från lång version till lättläst : – En komparativ textanalys av Maj Bylocks lättlästa version av Robinson Crusoe, samt en längre version av Robinson Crusoe". Thesis, Örebro universitet, Institutionen för humaniora, utbildnings- och samhällsvetenskap, 2017. http://urn.kb.se/resolve?urn=urn:nbn:se:oru:diva-58896.
Testo completoAbstract (sommario):
Denna uppsats är en komparativ textanalys av Maj Bylocks lättlästa version av Daniel Defoes Robinson Crusoe (1995) och en längre version av Daniel Defoe, översatt av Britt Edlund (2010). Syftet med analysen är att i jämförelse med den längre versionen, undersöka hur kriterier för en lättläst bok kommer till uttryck i Bylocks lättlästa version. Syftet är även att urskilja fördelar och nackdelar med den lättlästa versionen. Analysen görs utifrån olika kriterier för lättläst från Reichenberg och Lundberg (2008/2009) och Ahlén (2003), och analysens resultat diskuteras främst utifrån Wittings (1979) teorier om handling och språk i en lättläst bok.Analysens resultat visar att flera kriterier för en lättläst bok kommer till uttryck i Bylocks lättlästa version. Vissa kriterier synliggörs dock på ett mer otydligt sätt, bland annat handlingen, där flera detaljer försvunnit i Bylocks lättlästa version. Utifrån analysen visas flera positiva och negativa aspekter. På den positiva sidan visas bland annat att illustrationerna i Bylocks lättlästa version ger stöd till texten och att meningarna är så pass korta att läsaren hinner ta till sig eventuella svåra ord. På den negativa sidan visas bland annat att definitioner på okända ord inte finns med, samt att karaktärerna i boken ges en vag beskrivning vilket kan medföra svårigheter för läsaren att knyta an till karaktärerna.
28
Kim, Min. "A study of Franz Liszt's Totentanz piano and orchestra version, and piano solo version /". Lecture recital, recorded July 20, 2006, in digital collections. Access restricted to the University of North Texas campus. connect to online resource, 2007. http://digital.library.unt.edu/permalink/meta-dc-5409.
Testo completoAbstract (sommario):
Thesis (D.M.A.)--University of North Texas, 2007.System requirements: Adobe Acrobat Reader. Accompanied by 4 recitals, recorded July 9, 2001, Nov. 18, 2002, and Jan. 24, 2005, and July 20, 2006. Includes bibliographical references and discography (p. 32-34).
29
Kim, Min. "A study of Franz Liszt's Totentanz: Piano and orchestra version, and piano solo version". Thesis, University of North Texas, 2006. https://digital.library.unt.edu/ark:/67531/metadc5409/.
Testo completoAbstract (sommario):
Undoubtedly, Totentanz has been one of the most famous works by Franz Liszt. Totentanz has been recorded by many pianists and addressed in much of the vast literature about Liszt and his works; however, little research has been focused on this work. Most studies of Totentanz address only the historical background of the piece in relation to the theme based on Dies irae. Currently, there are no specific studies about the solo piano or two piano versions and only one recording was located. Liszt's own piano solo transcription of this famous work is an excellent addition to the concert repertoire. Totentanz consists of six variations that include canonic and fugato sections. The main theme is based on the Gregorian chant Dies irae, a melody that has been used by many other composers, most notably Berlioz in Witches Sabbath of Symphonie fantastique, op. 14 and Rachmaninoff in Rhapsody on a Theme of Paganini. This study contains five chapters. Chapters I and II provide background information, historical background and influences of Totentanz. Chapter III presents an outline of Liszt's achievement as a transcriber. Liszt revised his own works numerous times from the 1840s and 1850s, including Transcendental Etudes, Paganini Etudes, and piano and orchestra works. Like in the case of Totentanz, transcribed form piano and orchestra into piano solo, Liszt transcribed and paraphrased hundreds of other composers' works as well. Chapter IV discusses and compares the two main versions for solo piano and piano and orchestra. Form and harmonic language in particular the use of tritone in Totentanz is discussed. The adjustment required in transcribing the work for piano solo is discussed in detail, followed by a conclusion.
30
Lee, Soo Eun Handel George Frideric. "Handel's Messiah : a Korean version /". Thesis, Connect to this title online; UW restricted, 2005. http://hdl.handle.net/1773/11212.
Testo completo
31
Herrmann, Kai. "Multi-Schema-Version Data Management". Doctoral thesis, Saechsische Landesbibliothek- Staats- und Universitaetsbibliothek Dresden, 2017. http://nbn-resolving.de/urn:nbn:de:bsz:14-qucosa-231946.
Testo completoAbstract (sommario):
Modern agile software development methods allow to continuously evolve software systems by easily adding new features, fixing bugs, and adapting the software to changing requirements and conditions while it is continuously used by the users. A major obstacle in the agile evolution is the underlying database that persists the software system’s data from day one on. Hence, evolving the database schema requires to evolve the existing data accordingly—at this point, the currently established solutions are very expensive and error-prone and far from agile.
In this thesis, we present InVerDa, a multi-schema-version database system to facilitate agile database development. Multi-schema-version database systems provide multiple schema versions within the same database, where each schema version itself behaves like a regular single-schema database. Creating new schema versions is very simple to provide the desired agility for database development. All created schema versions can co-exist and write operations are immediately propagated between schema versions with a best-effort strategy. Developers do not have to implement the propagation logic of data accesses between schema versions by hand, but InVerDa automatically generates it.
To facilitate multi-schema-version database systems, we equip developers with a relational complete and bidirectional database evolution language (BiDEL) that allows to easily evolve existing schema versions to new ones. BiDEL allows to express the evolution of both the schema and the data both forwards and backwards in intuitive and consistent operations; the BiDEL evolution scripts are orders of magnitude shorter than implementing the same behavior with standard SQL and are even less likely to be erroneous, since they describe a developer’s intention of the evolution exclusively on the level of tables without further technical details. Having the developers’ intentions explicitly given in the BiDEL scripts further allows to create a new schema version by merging already existing ones.
Having multiple co-existing schema versions in one database raises the need for a sophisticated physical materialization. Multi-schema-version database systems provide full data independence, hence the database administrator can choose a feasible materialization, whereby the multi-schema-version database system internally ensures that no data is lost. The search space of possible materializations can grow exponentially with the number of schema versions. Therefore, we present an adviser that releases the database administrator from diving into the complex performance characteristics of multi-schema-version database systems and merely proposes an optimized materialization for a given workload within seconds. Optimized materializations have shown to improve the performance for a given workload by orders of magnitude.
We formally guarantee data independence for multi-schema-version database systems. To this end, we show that every single schema version behaves like a regular single-schema database independent of the chosen physical materialization. This important guarantee allows to easily evolve and access the database in agile software development—all the important features of relational databases, such as transaction guarantees, are preserved. To the best of our knowledge, we are the first to realize such a multi-schema-version database system that allows agile evolution of production databases with full support of co-existing schema versions and formally guaranteed data independence.
32
Johansson, Olof, e Daniel Persson. "Workflows and Distributed Version Control". Thesis, Blekinge Tekniska Högskola, Sektionen för datavetenskap och kommunikation, 2011. http://urn.kb.se/resolve?urn=urn:nbn:se:bth-3613.
Testo completoAbstract (sommario):
This bachelor thesis focuses on distributed version control systems and workflows used when working with such systems. The thesis will investigate benefits and disadvantages in using distributed version control in software development using a literature review of the available articles on the subject as well as a post-mortem analysis (with questionnaires and data collected from the build environment) of a student run software engineering project. We find that the migration costs are high, but that the advantages may outweigh its drawbacks for some. We conclude that many projects would benefit from migrating, but in particular, that new projects would not only benefit, but also not have high migration costs.En undersökning om hur man kan arbeta med moderna, distribuerade, versionshanteringssystem. Baserat på dels en literaturstudie, och dels en frågeundersökning bland studenter på BTH dras slutsatsen att det både finns för- och nackdelar, men att fördelarna verkar väga över i det generella fallet.
Jag (Olof Johansson) är nåbar främst per e-post, men även på telefonnummer +46739467135.
33
Samalens, Gomes Véronique. "Oedipus-Rex : une version mythique". Paris 4, 1987. http://www.theses.fr/1987PA040157.
Testo completoAbstract (sommario):
Cette étude se propose de dégager la nature mythique de la musique, ceci à travers l'exemple précis d'une œuvre, Oedipus-Rex de Stravinsky. En nous inspirant du modèle formel de l'œuvre pour construire notre travail et en nous aidant du mythe d'Œdipe pour réfléchir au procès de la connaissance, nous avons entrepris une enquête sémantique subjective qui procède à trois niveaux : un niveau analytique qui envisage l'opéra-oratorio à partir d'une vision contradictoire, celle du rite et celle du drame; un niveau exégétique qui intègre l'œuvre dans une double lecture, celle du compositeur et celle de l'auditeur; enfin un niveau théorique qui tente de définir ce que peut être une recherche sur le mythe et la musique. Considérant qu'Oedipus-Rex constitue une version du mythe d'Œdipe, c'est-à-dire une réalisation exprimant les interrogations d'une culture spécifique, nous l'envisageons donc avec ses propres critères, à savoir d'un point de vue esthétique et en fonction de la problématique de l'opéra, celle de la représentation humaine. A l'issue de cette entreprise, il apparait que la force mythique d'Oedipus-Rex est de proposer un schéma à la fois binaire et ambigu qui permette à l'œuvre de catalyser ce matériel symbolique collectif que constitue le mythiqueThe purpose of this study is to emphasize the mythical dimension of music, using as a particular instance one of Stravinsky’s works, namely Oedipus-rex. By using the formal structure of the piece to organize our research and by making use of the myth of Oedipus to reflect on the process of knowledge, we undertook a subjective semantic enquiry going on to three different levels. The analytical level considers the opera-cum-oratorio from a contradictory viewpoint taking in both ritual and drama. The exegetic level integrates the work in a double-sided reading, that of the composer and that of the listener. Finally, the theoretical level attempts to define what could be a research on myth and music. Considering that Oedipus-rex is one version of the myth of Oedipus, i. E. A product expressing the questioning of a specific culture, we consider it in the light of its own criteria. This implies having an aesthetic stand and looking into the problematic of the opera, as a human representation. At the end of our quest, it seems that the strength of Oedipus-rex as a myth is to offer both a binary and ambiguous scheme which enables the work to crystalize the collective and symbolic material of which myths are made of
34
Samalens-Gomes, Véronique. "Oedipus-Rex, une version mythique". Lille 3 : ANRT, 1988. http://catalogue.bnf.fr/ark:/12148/cb376055061.
Testo completo
35
Seppänen, V. (Vili). "Open source version control software". Bachelor's thesis, University of Oulu, 2015. http://jultika.oulu.fi/Record/nbnfioulu-201503311195.
Testo completoAbstract (sommario):
Abstract. The environment around open source version control software is very opinionated and therefore it is hard to find unbiased comparison between different open source version control software. This Bachelor’s thesis provides background and covers the basics of version control systems. Thesis also categorizes and differentiates the main types of version control systems, by investigating the way they handle repositories and by categorizing them to centralized and distributed. Finally, this thesis provides the unbiased technical comparison of the selected open source version control software and a way to map a suitable one for a software project. Comparison of technical details is collected into tables for easy interpretation and the main differentiators are explained more carefully. Mapping is achieved by pairing the major characteristics of different software projects with the technical features of version control systems and then with version control software that best supports these specific features. Pairing of the software project and the version control software is further refined with technical details that are not covered by the needs of major characteristics of the software project. Selection of the open source version control software is restricted to the four most popular ones.Avoimen lähdekoodin versionhallintaohjelmistot. Tiivistelmä. Avoimen lähdekoodin versionhallintaohjelmistoista on vaikea löytää puolueetonta vertailua, koska mielipiteet niiden ympärillä ovat hyvin polarisoituneita. Tämä kandidaatintyö tarjoaa taustatietoa ja käy läpi versionhallintajärjestelmien perusteet. Lisäksi tutkielma luokittelee ja erottaa versionhallintajärjestelmien päätyypit tarkastelemalla järjestelmien tapaa käyttää versionhallinnan varastoa ja kategorisoimalla järjestelmät sen mukaan keskitettyihin ja hajautettuihin. Tämä kandidaatintyö esittää myös puolueettoman teknisen vertailun yleisimmistä avoimen lähdekoodin versionhallintaohjelmistoista ja tavan kartoittaa ohjelmistoprojektiin sopiva versionhallintaohjelmisto. Tekninen vertailu on koottu taulukoiksi tulkinnan helpottamiseksi. Lisäksi pääeroavaisuudet on käyty läpi tarkemmin. Sopivan versionhallintaohjelmiston kartoittaminen ohjelmistoprojektiin on toteutettu yhdistämällä ohjelmistoprojektien tunnusomaiset piirteet versionhallintajärjestelmien ominaisuuksiin ja tämän jälkeen valitsemalla versionhallintaohjelmisto, joka toteuttaa kyseiset ominaisuudet. Tämän lisäksi versionhallintaohjelmiston valintaa tarkennetaan ottamalla huomioon ne tekniset eroavaisuudet, jotka eivät tule esiin tarkasteltaessa ohjelmistoprojektin tunnusomaisia piirteitä. Avoimen lähdekoodin versionhallintaohjelmistoista mukaan on valittu vain neljä käytetyintä.
36
Ostrovsky, N., e Wallace E. Jr Dixon. "Child Behavior Questionnaire: Ukrainian Version". Digital Commons @ East Tennessee State University, 2009. https://dc.etsu.edu/etsu-works/4935.
Testo completo
37
Reginato, Irène. "La Version K (catalane) du Devisement du Monde/ Milione de Marco Polo : recherches et éditions". Thesis, Paris, EPHE, 2016. http://www.theses.fr/2016EPHE4027.
Testo completoAbstract (sommario):
Le sujet de la thèse est la Version Catalane (K) du Devisement du Monde de Marco Polo. Rédaction abrégée, K est conservée dans une copie catalane Kc (Florence Ricc. 2048), une traduction française Kf (Rome, Bibliothèque du Vatican, Ott. Lat. 2207) et une traduction aragonaise Ka (Madrid, Escorial, Z. I. 2). La thèse présente une introduction en cinq chapitres. Le premier contient la description des manuscrits; le deuxième chapitre développe un stemma codicum, démontre l’existence de l’archétype Kx, en définit les erreurs et la langue. Le troisième chapitre se penche sur les erreurs de traduction et montre la descendance franco-italienne de la Version K. Le quatrième chapitre étudie K comme une version résumée, en mettant en relief les lacunes sur la base d’une comparaison avec la version de référence F (Paris, BNF, fr. 1116). Le cinquième chapitre, enfin, propose la localisation de K dans le stemma codicum général de l’œuvre. Ensuite, la thèse présente l’édition des trois témoins Kc, Kf et Ka, chacune accompagnée d’une analyse linguistique. La thèse se termine par la traduction critique en français de la Version KThe issue of the thesis is the Catalan Version (K) of the Devisement du Monde by Marco Polo. An abbreviated version, K is preserved in a Catalan copy called Kc (Florence, Ricc. 2048), a French translation named Kf (Rome, Vatican Library, Ott. lat. 2207) and an Aragonese translation called Ka (Madrid, Escorial, Z. I. 2). The thesis presents an introduction divided into five chapters. The first chapter contains the description of the manuscripts; the second chapter develops a stemma codicum, identifies the archetype Kx and defines its errors and language. The third chapter focuses on the translation mistakes, which demonstrate that the Catalan version had a Franco-Italian model. The fourth chapter studies K as text summary, highlighting its gaps on the basis of a comparison with the reference version F (Paris, BNF, fr. 1116). The fifth chapter, finally, proposes a localization of K in the general stemma codicum of the work. After the introduction, the thesis presents the edition of the three copies, each one accompanied by a linguistic analysis. The thesis ends with the critical translation, in modern French, of the Catalan Version K
38
SAFFON, MARIE-PIERRE. "Mini-mental-state de folstein : comparaison entre une version standardisee et une version avec facilitations". Toulouse 3, 1992. http://www.theses.fr/1992TOU31088.
Testo completo
39
Soazo, Ahumada Christian Andrés Verfasser], e Walter Bruno [Akademischer Betreuer] [Berg. "Una historia "salvaje": re-versión de la modernidad, vanguardia y globalización en la obra de Roberto Bolaño = Eine „wilde" Geschichte: Re-version der Moderne, Avantgarde und Globalisierung im Werk Roberto Bolaños". Freiburg : Universität, 2013. http://d-nb.info/1114144991/34.
Testo completo
40
Hoffmann-Ostenhof, M., T. Hoffmann-Ostenhof, A. Laptev e thoffman@esi ac at. "A Geometrical Version of Hardy's Inequality". ESI preprints, 2001. ftp://ftp.esi.ac.at/pub/Preprints/esi1017.ps.
Testo completo
41
Kleine, Matthias, Robert Hirschfeld e Gilad Bracha. "An abstraction for version control systems". Universität Potsdam, 2011. http://opus.kobv.de/ubp/volltexte/2012/5562/.
Testo completoAbstract (sommario):
Versionsverwaltungssysteme (VCS) ermöglichen es Entwicklern, Änderungen an Softwareartifakten zu verwalten. VCS werden mit Hilfe einer Vielzahl verschiedener Werkzeuge bedient, wie z.,B. graphische Front-ends oder Kommandozeilenwerkzeuge. Es ist wünschenswert mit einzelnen solcher Werkzeuge unterschiedliche VCS bedienen zu können.
Bislang hat sich jedoch keine Abstraktion für Versionsverwaltungssysteme durchgesetzt, mit deren Hilfe solche Werkzeuge erstellt werden können. Stattdessen implementieren Werkzeuge zur Interaktion mit mehreren VCS ad-hoc Lösungen.
Diese Masterarbeit stellt Pur vor, eine Abstraktion über Versionsverwaltungskonzepte. Mit Hilfe von Pur können Anwendungsprogramme entwickelt werden, die mit mehreren Versionsverwaltungssystemen interagieren können. Im Rahmen dieser Arbeit wird eine Implementierung dieser Abstraktion bereitgestellt und mit Hilfe eines Anwendungsprogramms validiert.Version Control Systems (VCS) allow developers to manage changes to software artifacts. Developers interact with VCSs through a variety of client programs, such as graphical front-ends or command line tools. It is desirable to use the same version control client program against different VCSs. Unfortunately, no established abstraction over VCS concepts exists. Instead, VCS client programs implement ad-hoc solutions to support interaction with multiple VCSs. This thesis presents Pur, an abstraction over version control concepts that allows building rich client programs that can interact with multiple VCSs. We provide an implementation of this abstraction and validate it by implementing a client application.
42
Lindström, Angelica, e Emilia Madebrink. "En uppdaterad version av läsbarhetsformeln LIX". Thesis, KTH, Skolan för datavetenskap och kommunikation (CSC), 2014. http://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-157571.
Testo completoAbstract (sommario):
Det finns mängder med faktorer som påverkar en texts läsbarhet. I denna rapport kommer flertalet faktorer undersökas inför skapandet av en ny läsbarhetsalgoritm. Målet är att denna algoritm ska vara bättre än LIX som är en algoritm konstruerad för svenska texter. Målet uppnåddes med en liten marginal men ytterligare förbättringar kan och bör implementeras.There are a lot of factors that affect readability. In this report some of these factors will be examined with the purpose of creating a new readability algoritm for Swedish texts. The goal of this projekt is for this new algoritm to surpass the traditional readability algorithm for Swedish; LIX. The conclusionis that the previously stated goal was reached only by a small marigin, the algorithm could and should be improved.2
43
Adams, Charles A. "VS : an optimistic version management system /". Full text open access at:, 1990. http://content.ohsu.edu/u?/etd,198.
Testo completo
44
AlDrobi, Molham Rateb. "Final version : uncertainty in artificial intelligence". Thesis, McGill University, 1993. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=69581.
Testo completoAbstract (sommario):
Reasoning with uncertain information has received a great deal of attention recently, as this issue has to be addressed when developing many expert systems.In this thesis we study the literature of uncertainty in AI. The approaches taken by the researchers in this field can be classified into two categories: non-numeric approaches and numeric approaches. From non-numeric methods, we summarize The Theory of Endorsements, and non-monotonic logics. From numeric methods, we elaborate on MYCIN certainty Factors, Dempster-Shafer Theory, Fuzzy Logic, and Probabilistic Approach. We point out that probability theory is an adequate approach if we interpret probability values as beliefs and not only as frequencies.
We first discuss broad and more thoroughly researched areas. We then focus more on integrating probability and logic as we believe this is a crucial approach to build up a setting for reasoning with uncertain information based on strong local foundations. Some key works in that area are traced back to 1913 when Lukasiewics published his paper on Logical Foundation of Probability. Comparisons between Nilsson's probabilistic logic and the related work of Quinlan, Grosof, McLeish, Chen, and Bacchus are given. We conclude the thesis by our remarks and suggestions for possible future research topics.
45
Icen, Ilhan. "A 2-dimensional version of holonomy". Thesis, Bangor University, 1995. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.318945.
Testo completo
46
Poon, Hon-fong, e 潘漢芳. "Dating the extant version of Kongcongzi". Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 2008. http://hub.hku.hk/bib/B40987905.
Testo completo
47
Florida-James, Barry. "Version control in engineering design databases". Thesis, University of Newcastle Upon Tyne, 2001. http://hdl.handle.net/10443/720.
Testo completoAbstract (sommario):
This thesis is concerned with lifecycle data support for the design of large made to order products. These products have so many complex functions to perforrn that no one designer will have all of the relevant skills such as in structural design Z): or electrical engineering to produce a comprehensive design. This therefore leads to the utilisation of a team of designers who will not only fulfil logically different design roles but often work at different physical locations. In such a design environment there may be several local models, represented in local Z-- databasesT. hese databases may or may not support versioning either of the data or of the schema which evolves as the product design grows. The interfaces to these databases ID will be varied as they are intended to suit the local needs of the design aIgDe nt. This thesis proposes a model for version control in a desig4nn environment which does not alter the designers existing view. Cý tý A system of distributed co-operatinZg:, aZgD ents is presented whose goal is to manatDg e change and orgCaIDni se version sets in an enrgDin eering environment. The agents are designed for full lifecycle support and inter-operation across heterogeneous networks. The agent communication is based on CORBA but an extra messaging layer is developed which utilises a language built in VDM-SL (Vienna Development Method - Specification Language). A version model is presented in two ways informally based on the assumptions on a general design process and formally in VDM-SL. ZP tP In order to demonstrate the effectiveness of the version model, two industrial case studies are presented. The first of these is a study of offshore process engineering. The second is a study of conceptual ship design.
48
Liburd, Soyini (Soyini Denise) 1980. "An N-version electronic voting system". Thesis, Massachusetts Institute of Technology, 2004. http://hdl.handle.net/1721.1/28441.
Testo completoAbstract (sommario):
Thesis (M. Eng.)--Massachusetts Institute of Technology, Dept. of Electrical Engineering and Computer Science, 2004.Includes bibliographical references (p. 103-109).
The ballot battles of the 2000 US Presidential Election clearly indicate that existing voting technologies and processes are not sufficient to guarantee that every eligible voter is granted their right to vote and implicitly to have that vote counted, as per the fifteenth, nineteenth, twenty fourth and twenty sixth amendments to the US constitution [1-3]. Developing a voting system that is secure, correct, reliable and trustworthy is a significant challenge to current technology [3, 4]. The Secure Architecture for Voting Electronically (SAVE) demonstrates that N-version programming increases the reliability and security of its systems, and can be used to increase the trustworthiness of systems. Further, SAVE demonstrates how a viable practical approach to voting can be created using N-version programming. SAVE represents a significant contribution to voting technology research because of its design, and also because it demonstrates the benefits of N-version programming and introduces these benefits to the field of voting technology.
by Soyini D. Liburd.
M.Eng.
49
Jordan, Alex. "A super version of Zhu's theorem /". Connect to title online (Scholars' Bank) Connect to title online (ProQuest), 2008. http://hdl.handle.net/1794/8283.
Testo completoAbstract (sommario):
Thesis (Ph. D.)--University of Oregon, 2008.Typescript. Includes vita and abstract. Includes bibliographical references (leaves 40-41). Also available online in Scholars' Bank; and in ProQuest, free to University of Oregon users.
50
Young, John P. "Multimedia Blake World Wide Web version /". View online record:, 2000. http://srproj.lib.calpoly.edu/jyoung.
Testo completo