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Letteratura scientifica selezionata sul tema "Wharton’s jelly"

Autore: Grafiati

Pubblicato: 4 giugno 2021

Ultima modifica: 1 febbraio 2022

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Articoli di riviste sul tema "Wharton’s jelly"

Amin, Sapna, Shripad Hebbar, Deepika Pothakamuri e Prashant Adiga. "Significance of Wharton’s jelly area in prediction of aberrant foetal growth". International Journal of Reproduction, Contraception, Obstetrics and Gynecology 7, n. 7 (27 giugno 2018): 2820. http://dx.doi.org/10.18203/2320-1770.ijrcog20182888.

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Background: Size of the baby at the time birth determines its outcome. Low birth weight babies have their own set of problems such as respiratory distress syndrome, hypoxic ischemic encephalopathy, metabolic derangements and high rates of admission to intensive care units. On the other hand too large babies may cause difficulty in vaginal births, higher incidence of birth trauma including the maternal genital injuries. Both conditions are associated with higher rates of operative delivery and hence it is important to investigate parameters which could identify these foetal growth abnormalities in the antenatal period only. The objective of the present investigation was to study the relationship between foetal umbilical cord Wharton’s Jelly Area and neonatal birth weight within two weeks of deliveryMethods: A prospective observational study was conducted at Department of Obstetrics and Gynaecology, Kasturba Medical College, Manipal, Karnataka, India over a period of two years. Two hundred and fifty women from 34 weeks gestational age who have delivered within two weeks of estimation of Wharton’s Jelly Area by ultrasound were analysed. Wharton’s Jelly Area was measured in pregnant woman after 34 weeks of gestation at the time of third trimester scan. Scans were repeated every two weeks till the woman delivered. Measurements were done in a free loop of the umbilical cord. Regression analysis was used to correlate Wharton’s jelly quantity with the birth weight obtained after birth of the neonate.Results: There was a good correlation between Wharton’s Jelly Area and neonatal birth weight. The mean birth weight was 2247.2 gms in <than 10th centile group, 2945.1gms in 10th to 90th centile and 3552.1 gms in more than 90th centile group, demonstrating a consistent rise in mean birth weight with higher centile groups. Polynomial regression function showed good fit between Wharton’s jelly and birth weight (R² = 0.8842, p<0.001). When Wharton’s jelly area was less than 10th centile, 72% of neonates had small for gestational age (SGA).Conclusions: There is a positive association between Wharton’s Jelly Area and neonatal birth weight. Birth weight of neonate showed steady increase with increasing Wharton’s Jelly Area.

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Seo, Min-Soo, Kyung-Ku Kang, Se-Kyung Oh, Soo-Eun Sung, Kil-Soo Kim, Young-Sam Kwon e Sungho Yun. "Isolation and Characterization of Feline Wharton’s Jelly-Derived Mesenchymal Stem Cells". Veterinary Sciences 8, n. 2 (7 febbraio 2021): 24. http://dx.doi.org/10.3390/vetsci8020024.

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Wharton’s jelly is a well-known mesenchymal stem cell source in many species, including humans. However, there have been no reports confirming the presence of mesenchymal stem cells in Wharton’s jelly in cats. The purpose of this study was to isolate mesenchymal stem cells (MSCs) from the Wharton’s jelly of cats and to characterize stem cells. In this study, feline Wharton’s jelly-derived mesenchymal stem cells (fWJ-MSCs) were isolated and successfully cultured. fWJ-MSCs were maintained and the proliferative potential was measured by cumulative population doubling level (CPDL) test, scratch test, and colony forming unit (CFU) test. Stem cell marker, karyotyping and immunophenotyping analysis by flow cytometry showed that fWJ-MSCs possessed characteristic mesenchymal stem cell markers. To confirm the differentiation potential, we performed osteogenic, adipogenic and chondrogenic induction under each differentiation condition. fWJ-MSCs has the ability to differentiate into multiple lineages, including osteogenic, adipogenic and chondrogenic differentiation. This study shows that Wharton’s jelly of cat can be a good source of mesenchymal stem cells. In addition, fWJ-MSCs may be useful for stem cell-based therapeutic applications in feline medicine.

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Gogiel, Tomasz, Edward Bańkowski e Stefan Jaworski. "Proteoglycans of Wharton’s jelly". International Journal of Biochemistry & Cell Biology 35, n. 10 (ottobre 2003): 1461–69. http://dx.doi.org/10.1016/s1357-2725(03)00128-6.

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Murphy, Sarah J., Nikita Deegan, Bobby D. O'Leary e Peter McParland. "Absence of Wharton’s jelly". BMJ Case Reports 13, n. 11 (novembre 2020): e237222. http://dx.doi.org/10.1136/bcr-2020-237222.

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Wharton’s jelly is a specialised tissue which surrounds the vasculature within the fetal umbilical cord. We present the case of a 42-year-old woman who gave birth to a female infant via emergency caesarean section. At the time of delivery, absence of Wharton’s jelly was noted. This finding was confirmed by histological examination. Emergency caesarean section was necessitated due to a fetal bradycardia, and of note, the patient had presented twice prior to this with reduced fetal movements.

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Dhitiseith, D., e S. Honsawek. "Differential Expression of Osteogenic Differentiation in Human Umbilical Cord Wharton’s Jelly-Derived Mesenchymal Stem Cells Treated with Demineralized Bone". Advanced Materials Research 55-57 (agosto 2008): 697–700. http://dx.doi.org/10.4028/www.scientific.net/amr.55-57.697.

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Mesenchymal stem cells are multipotential cells capable of differentiating into osteoblasts, chondrocytes, adipocytes, tenocytes, and myoblasts. Wharton’s jelly consists of stem cells that are a rich source of primitive multipotent mesenchymal cells. Demineralized bone matrix (DBM) has been widely utilized as a biomaterial to promote new bone formation. We isolate and characterize umbilical cord Wharton’s Jelly-derived mesenchymal stem (UCMS) cells derived from Wharton’s jelly and examine the biological activity of DBM in this cell line. Osteoblast differentiation of the UCMS cells was determined using alkaline phosphatase (ALP) activity assay. To examine differential gene expression during osteogenic differentiation, total RNA was isolated from UCMS cells in the absence or presence of DBM on day7 and analyzed using osteogenesis cDNA gene array. The selected genes were verified using reverse transcriptase-polymerase chain reaction (RT-PCR) analyses. Wharton’s jelly derived cells could differentiate along an osteogenic lineage after treatment of DBM. The ALP activity assay showed that human UCMS cells could differentiate into osteogenic lineage. Gene expression of human UCMS cells treated with DBM for 7 days was analyzed by using cDNA array and RT-PCR analyses. We found that expression of RUNX2 and SMAD2 was upregulated whereas SMAD7 expression was downregulated as confirmed by RT-PCR. UCMS cells from a Wharton’s jelly of human umbilical cord could express osteogenesis genes for treatment with DBM. Wharton’s jelly from umbilical cord is a new source of mesenchymal stem cells that are readily available for application to bone tissue engineering.

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Świstowska, Małgorzata, Paulina Gil-Kulik, Arkadiusz Krzyżanowski, Tomasz Bielecki, Marcin Czop, Anna Kwaśniewska e Janusz Kocki. "Potential Effect of SOX2 on the Cell Cycle of Wharton’s Jelly Stem Cells (WJSCs)". Oxidative Medicine and Cellular Longevity 2019 (2 giugno 2019): 1–8. http://dx.doi.org/10.1155/2019/5084689.

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The connective tissue of the umbilical cord contains stem cells called Wharton’s jelly cells. These cells express core transcription factors (NANOG, OCT4, and SOX2). The protein product of the SOX2 gene controls the cell cycle by interacting with cyclin D (directly and indirectly) and cycle inhibitors—p21 and p27, as well as two E2f3 protein isoforms. The aim of the study was to analyze the effect of SOX2 on the cell cycle of stem cells of Wharton’s jelly. The material for the study was the stem cells of Wharton’s jelly isolated from 20 umbilical cords collected during childbirth. The stem cells collected were subjected to cytometric analysis, cell culture, and RNA isolation. cDNA was the starting material for the analysis of gene expression: SOX2, CCND1, CDK4, and CDKN1B. The studies indicate a high proliferative potential of the Wharton’s jelly stem cells and the inhibitory effect of SOX2 on the expression of the CCND1 and CDK4 gene.

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Rajasekharan, Sreekumar, UmesanKannanvilakom Govindapillai, Manju Madhavan C., Suja R. S., Swapna T e Sajeena Narayanan Chitradevi. "To Estimate the Importance of Wharton’s Jelly in the Growth of the Foetus – A Light Microscopic Study". Journal of Evolution of Medical and Dental Sciences 10, n. 35 (30 agosto 2021): 3024–29. http://dx.doi.org/10.14260/jemds/2021/617.

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BACKGROUND Human umbilical cord contains two arteries and one vein with their tunica intima and tunica media layers. The role of tunica adventitia is fulfilled by Wharton’s jelly, a mucoid connective tissue. The function of Wharton’s jelly is to prevent the vessels from compression and torsion which is essential for foetal development. The purpose of the study was to estimate the importance of Wharton’s jelly in the growth of the foetus. METHODS Umbilical cord tissue collected from each case was immediately put in 10 % formalin for fixation. Slides were then stained with Haematoxylin and Eosin. These slides were then read under light microscopy and measurements were taken using a photomicrograph. Wharton’s jelly area was calculated by subtracting the total vessel area from the umbilical cord area. RESULTS The histological measurements of umbilical vessels include the external diameter, lumen diameter, wall thickness, thickness of tunica intima and tunica media, and the area. The mean area of the umbilical cord was 35.73 ±23.04 mm2 (Mean ± SD) and the mean area of the Wharton’s jelly was 29.74 ± 19.26 mm2. There was a significant difference in the external diameter and wall thickness of the umbilical artery. Analyses showed that there was a significantly (P < 0.01) increased external diameter and wall thickness of umbilical artery in normal cases, compared to single umbilical artery cases. CONCLUSIONS There was a significant positive correlation between the gestational age and the external diameter of the umbilical cord. There was a significant difference in the external diameter of the umbilical cord between SUA cases (4.45 mm) and the other foetuses with normal umbilical cord (6.53 mm). There was a significantly increased external diameter, lumen diameter, wall thickness and area of umbilical vein in normal cases, compared to single umbilical artery cases. There was a significantly increased area of umbilical cord and area of Wharton’s jelly in normal umbilical cord foetuses than foetuses with a single umbilical artery. KEY WORDS Foetus, Umbilical Cord, Wharton’s Jelly, Umbilical Artery, Umbilical Vein, Light Microscopy

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Stocco, Elena, Silvia Barbon, Daniele Dalzoppo, Silvano Lora, Leonardo Sartore, Marcella Folin, Pier Paolo Parnigotto e Claudio Grandi. "Tailored PVA/ECM Scaffolds for Cartilage Regeneration". BioMed Research International 2014 (2014): 1–12. http://dx.doi.org/10.1155/2014/762189.

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Articular cartilage lesions are a particular challenge for regenerative medicine due to cartilage low self-ability repair in case of damage. Hence, a significant goal of musculoskeletal tissue engineering is the development of suitable structures in virtue of their matrix composition and biomechanical properties. The objective of our study was to designin vitroa supporting structure for autologous chondrocyte growth. We realized a biohybrid composite scaffold combining a novel and nonspecific extracellular matrix (ECM), which is decellularized Wharton’s jelly ECM, with the biomechanical properties of the synthetic hydrogel polyvinyl alcohol (PVA). Wharton’s jelly ECM was tested for its ability in promoting scaffold colonization by chondrocytes and compared with polyvinyl alcohol itself and the more specific decellularized cartilage matrix. Our preliminary evidences highlighted the chance of using Wharton’s jelly ECM in combination with PVA hydrogels as an innovative and easily available scaffold for cartilage restoration.

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Behera, Shashi Shankar, Shashi Shankar Behera e Prafulla Kumar Chinara. "EVALUATION OF FETAL WEIGHT SONOGRAPHICALLY USING AREA OF WHARTON’S JELLY AND MORPHOLOGY OF UMBILICAL CORD". Asian Journal of Pharmaceutical and Clinical Research 10, n. 10 (1 settembre 2017): 253. http://dx.doi.org/10.22159/ajpcr.2017.v10i10.20037.

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Objective: To establish a sonographic relationship between Area of Wharton’s Jelly (AWJ) and umbilical cord morphometry with the birth weight of the fetus in low-risk pregnancies from 13 to 40 weeks.Methods: A total of 800 singleton pregnant females were subjected for routine sonographic evaluation. The umbilical cord length, diameter, and AWJ were determined. The gestational age and fetal weight were determined using usual fetal parameters. Umbilical cord morphometry along with Area of Wharton Jelly can be utilized as other parameters to increase the accuracy of fetal weight.Results: In our study, the umbilical cord diameter at birth showed statistically significant positive correlation with birth weight (R=0.167; p<0.001). Umbilical cord length, diameter, and Area of Wharton Jelly showed statistically significant positive correlation with birth weight (p<0.001).Conclusion: Using statistical analysis, a positive correlation was established between estimated fetal weight and fetal age with umbilical cord morphometry and AWJ.

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Stefańska, Katarzyna, Katarzyna Ożegowska, Greg Hutchings, Małgorzata Popis, Lisa Moncrieff, Claudia Dompe, Krzysztof Janowicz et al. "Human Wharton’s Jelly—Cellular Specificity, Stemness Potency, Animal Models, and Current Application in Human Clinical Trials". Journal of Clinical Medicine 9, n. 4 (12 aprile 2020): 1102. http://dx.doi.org/10.3390/jcm9041102.

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Stem cell therapies offer a great promise for regenerative and reconstructive medicine, due to their self-renewal and differentiation capacity. Although embryonic stem cells are pluripotent, their utilization involves embryo destruction and is ethically controversial. Therefore, adult tissues that have emerged as an alternative source of stem cells and perinatal tissues, such as the umbilical cord, appear to be particularly attractive. Wharton’s jelly, a gelatinous connective tissue contained in the umbilical cord, is abundant in mesenchymal stem cells (MSCs) that express CD105, CD73, CD90, Oct-4, Sox-2, and Nanog among others, and have the ability to differentiate into osteogenic, adipogenic, chondrogenic, and other lineages. Moreover, Wharton’s jelly-derived MSCs (WJ-MSCs) do not express MHC-II and exhibit immunomodulatory properties, which makes them a good alternative for allogeneic and xenogeneic transplantations in cellular therapies. Therefore, umbilical cord, especially Wharton’s jelly, is a promising source of mesenchymal stem cells.

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Più fonti

Tesi sul tema "Wharton’s jelly"

Seshareddy, Kiran Babu. "Human Wharton’s jelly cells-isolation and characterization in different growth conditions". Thesis, Kansas State University, 2008. http://hdl.handle.net/2097/1054.

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Master of Science
Department of Anatomy and Physiology
Mark L. Weiss
Wharton's jelly is a non-controversial source of mesenchymal stromal cells. Isolation of the cells is non-invasive and painless. The cells have been shown to have a wide array of therapeutic applications. They have improved symptoms when transplanted in a variety of animal disease models, can be used in tissue engineering applications to grow living tissue ex vivo for transplantation, and can be used as drug delivery vehicles in cancer therapy. The cells have also been shown to be non-immunogenic and immune suppressive. This thesis focuses on optimizing isolation protocols, culture protocols, cryopreservation, and characterization of cells in different growth conditions. Results from the experiments indicate that isolation of cells by enzyme digestion yields cells consistently, a freezing mixture containing 90% FBS and 10% DMSO confers maximum viability, and the expression of mesenchymal stromal cell consensus markers does not change with passage and cryopreservation. The results of the experiments also show that cells grow at a higher rate in 5% oxygen culture conditions compared to 21% oxygen culture conditions, serum does not have an effect on growth of the cells, serum and oxygen do not have effects on the expression of mesenchymal stromal cell consensus markers and the cells are stable without nuclear abnormalities when grown in 5% oxygen and serum free conditions for six passages after first establishing in serum conditions.

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Morton, Jodi Mirissa. "Effects of intrauterine growth restriction on Wharton’s jelly cells and preweaning traits in pigs". Diss., Kansas State University, 2018. http://hdl.handle.net/2097/38757.

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Doctor of Philosophy
Department of Animal Sciences and Industry
Duane L. Davis
Intrauterine growth restriction (IUGR) affects all mammals. In the swine industry IUGR pigs result from intrauterine crowding. Prenatal programming in IUGR pigs has substantial effects on myogenesis and adipogenesis. Prenatal programming due to IUGR is also a problem in humans and long-term effects on adipogenesis are well established for small for gestational age (SGA) babies. Mesenchymal stem cells (MSCs) are the precursors for adipocytes. The umbilical cord contains a population of MSCs in Wharton’s jelly (WJ) and they can be harvested postnatally without ethical issues. Therefore, WJMSCs are proposed as models for studying prenatal programming of adipogenesis. We selected genes from studies of adipogenesis in humans and other species and examined their expression in pig WJ. We assigned pigs within litter as High, Medium, or Low birth weight and evaluated these categories for expression of Cox1, Cox2, EGR1, PPARɣ1, PPARɣ2, and Pref1. Differences due to size classification within litter were limited but there were correlations between weaning weight and delta cycle threshold (ΔCt) for EGR1 (r = 0.28; P < 0.009), PPARɣ1 (r = 0.29; P < 0.007), and PPARɣ2 (r = 0.30; P < 0.005). This may be consistent with the reports for SGA babies where EGR1 is upregulated by prenatal growth restriction. To gain insight into when during pregnancy IUGR affects WJ cells we collected umbilical cords at d 60 and d 95. In d 60 umbilical cords, small fetuses had increased (P = 0.06) Cox1 gene expression. We tested the ability of d 60 WJ cells to undergo adipogenic differentiation using standard protocols and a cycling protocol that exposed the cells to adipogenic differentiation conditions interposed with a rest phase with high insulin. It has been reported that the cycling protocol revealed increased glucose uptake in WJ cells from human SGA babies. We found that d 60 WJ cells did not show adipogenic differentiation in any of the protocols tested however glucose uptake correlated negatively with birth weight at Cycle 0 (P < 0.02; r = 0.61). In summary, pig WJ cells reveal some effects of IUGR but they appear to differ from the relationship demonstrated reported for human SGA babies. A new finding was that at midgestation pig WJ cells do not appear to be competent to complete adipogenesis. We also studied nursing managements to improve outcomes for IUGR pigs. Colostrum intake may be a problem, particularly for light weight pigs and those born later during farrowing. Split suckling is the removal of some pigs to allow others unrestricted nursing access. We temporarily removed the six heaviest pigs and this treatment increased gain and weight by d 7 of age. Colostrum intake was highest for the high birth weight pigs. When we temporarily removed the first half of the litter, colostrum intake was increased for the second half of litter born and the difference in immunocrit was reduced between the two litter halves.

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Reeds, Kimberly. "In vitro effects of canine Wharton’s jelly mesenchymal stromal cells and nanoparticles on canine osteosarcoma D17 cell viability". Thesis, Kansas State University, 2011. http://hdl.handle.net/2097/11990.

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Master of Science
Department of Clinical Sciences
Mary Lynn Higginbotham
Objectives – To isolate and maintain canine Wharton’s jelly mesenchymal stromal cells (WJMSCs) in culture, to determine the effects of micellar nanoparticles containing doxorubicin (DOX) on WJMSCs and canine osteosarcoma (OSA) D17 cell viability, and to determine the effects of conditioned media from WJMSCs loaded with micellar nanoparticles containing DOX on OSA D17 cell viability. Sample Population – Canine WJMSCs containing various concentrations of DOX micelles and canine OSA D17 cells. Procedures – WJMSCs were isolated from canine umbilical cords. Micellar nanoparticles containing DOX were prepared and added to culture plates containing canine OSA D17 cells to determine micelle effects on cell growth and viability. Conditioned media from culture plates containing canine WJMSCs incubated with various DOX micelle concentrations was added to OSA D17 cells for conditioned media experiments. A 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay was performed to assess OSA D17 cell viability. A trypan blue stain was also utilized to perform cell counts to determine the effect of the DOX micelles on stromal cell growth. Results – WJMSCs were successfully isolated and maintained in culture. Micellar nanoparticles containing DOX decreased OSA D17 cell viability. OSA D17 cell viability was also decreased following incubation with conditioned media from canine WJMSCs loaded with micellar nanoparticles containing DOX. Significant decreases with the conditioned media of canine WJMSCs loaded with 10μM micelles occurred at 48 hours (p < 0.005) and at 72 and 96 hours (p < 0.0001). Significant decreases were also observed with the 1 μM DOX micelles at 72 hours (p < 0.005) and 96 hours (p < 0.0001). WJMSC numbers decreased in a dose dependent manner following incubation with DOX micelles. Changes in WJMSC number was not caused by increased cell death as all variables produced similar percentages of dead cells. Conclusions – Canine WJMSCs were successfully isolated and maintained in culture. Stromal cells containing DOX micellar nanoparticles induced OSA D17 cell cytotoxicity while inducing an anti-proliferative, rather than cytotoxic effect, on the WJMSC. These data support future in vivo experiments utilizing canine WJMSCs and micellar nanoparticles.

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Sankaramaddi, Jeevan Reddy [Verfasser]. "Evaluation of human umbilical cord Wharton’s Jelly Cells as a potential cell source for cardiovascular tissue engineering / Jeevan Reddy Sankaramaddi". Berlin : Medizinische Fakultät Charité - Universitätsmedizin Berlin, 2017. http://d-nb.info/1148425780/34.

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Ferreira, Daniela Filipa Alves. "Influence of substrates composition on immunomodulation by MSCs". Master's thesis, Universidade de Aveiro, 2016. http://hdl.handle.net/10773/16048.

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Mestrado em Biomedicina Molecular
Mesenchymal stem cells (MSCs) are non-hematopoietic multipotent stem cells capable to self-renew and differentiate along different cell lineages. MSCs can be found in adult tissues and extra embryonic tissues like the umbilical cord matrix/Wharton’s Jelly (WJ). The latter constitute a good source of MSCs, being more naïve and having a higher proliferative potential than MSCs from adult tissues like the bone marrow, turning them more appealing for clinical use. It is clear that MSCs modulate both innate and adaptive immune responses and its immunodulatory effects are wide, extending to T cells and dendritic cells, being therapeutically useful for treatment of immune system disorders. Mechanotransduction is by definition the mechanism by which cells transform mechanical signals translating that information into biochemical and morphological changes. Here, we hypothesize that by culturing WJ-MSCs on distinct substrates with different stiffness and biochemical composition, may influence the immunomodulatory capacity of the cells. Here, we showed that WJ-MSCs cultured on distinct PDMS substrates presented different secretory profiles from cells cultured on regular tissue culture polystyrene plates (TCP), showing higher secretion of several cytokines analysed. Moreover, it was also shown that WJ-MSCs cultured on PDMS substrates seems to possess higher immunomodulatory capabilities and to differentially regulate the functional compartments of T cells when compared to MSCs maintained on TCP. Taken together, our results suggest that elements of mechanotransduction seem to be influencing the immunomodulatory ability of MSCs, as well as their secretory profile. Thus, future strategies will be further explored to better understand these observation and to envisage new in vitro culture conditions for MSCs aiming at distinct therapeutic approaches, namely for immune-mediated disorders.
As células estaminais mesenquimais (MSCs) são células não-hematopoéticas, multipotentes, capazes de se auto-renovar e de diferenciar em diferentes tipos celulares. As MSCs estão presentes em tecidos mesenquimais e de tecidos extra embrionários, tais como a matriz do cordão umbilical/Wharton’s Jelly(WJ). Estes últimos constituem uma boa fonte de de MSCs, sendo estas mais naive e tendo um maior potencial de proliferação do que as MSCs obtidas de tecidos adultos, como a medula óssea, tornando as MSCs da matriz do cordão umbilical/Wharton’s Jelly sejam mais apelativas para uso clínico. As MSCs possuem a capacidade de modularem tanto o sistema imune inato como o adquirido e os seus efeitos são vastos, afectando todas as células do sistema imune. Esta capacidade é bastante vantajosa para o uso terapêutico destas células em doenças do sistema imunitário. A mecanotransducção é por definição o mecanismos pelo qual as células convertem estímulos mecânicos em uma resposta bioquímica e com mudanças na sua morfologia. Apartir destas observações colocámos a hipotese de que mantendo MSCs in vitro em diferentes substratos poderia influencia a sua capacidade imunomoduladora. Com este trabalho, demonstrámos que ao plaquear MSCs em diferentes substratos de PDMS, estas mostram uma tendência para secretar quantidades diferentes de vários factores soluveis analisados, relativamente a MSCs mantidas em cultura em plataformas convencionais (placas de cultura de células - TCP). Para além disto, foi também observado que MSCs plaqueadas em substratos de PDMS aparentavam possuir uma maior capacidade imunomoduladora quando comparadas com MSCs mantidas em condições convencionais. Em conjunto todos os resultados obtidos sugerem que elementos relacionados com a mecanotransdução parecem influenciar a capacidade imunomoduladora de MSCs e a sua secreção de factores solúveis. Deste modo, estudos futuros poderão elucidar os mecanismos responsáveis por estas observações, de modo a permitir que se possa constitutuir melhores estratégias de cultura de MSCs para futuro uso terapêutico dirigido a doenças do sistema imunitário.

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Laroye, Caroline. "Le cordon ombilical : une source alternative de cellules souches/stromales mésenchymateuses dans le traitement du choc septique ?" Thesis, Université de Lorraine, 2017. http://www.theses.fr/2017LORR0279.

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Le choc septique est actuellement la dixième cause de mortalité à travers le monde à égalité avec les infarctus du myocarde. Sa physiopathologie extrêmement complexe, entrelaçant un état pro-inflammatoire et anti-inflammatoire, rend caduque l’action des thérapeutiques conventionnelles. En ce sens, les recherches s’orientent vers les thérapeutiques innovantes et notamment les cellules souches/stromales mésenchymateuses (CSM). En effet, les études murines ont mis en évidence que les CSM étaient en mesure, notamment par leurs actions paracrines, d’améliorer la survie, la défaillance d’organes mais également la bactériémie de souris soumises à un choc septique. Cependant, les propriétés des CSM varient en fonction du tissu dont elles sont issues et particulièrement selon qu’elles proviennent de tissus fœtaux (cordon ombilical, placenta, liquide amniotique) ou adultes (moelle osseuse, tissu adipeux...). Ainsi, notre premier objectif a été de comparer, dans un modèle murin de choc septique, l’action des CSM issues de la moelle osseuse (MO) à celle des CSM issues de la gelée de Wharton (GW) du cordon ombilical. Cette étude murine a permis de mettre en évidence une action quelque peu différente, entre les CSM-GW et les CSM-MO, sur la physiopathologie du choc septique sans que pour autant, l’une des deux sources de CSM, ne se dégage significativement de l’autre en termes d’efficacité. Cependant, en raison de leur importante capacité de prolifération et de l’accessibilité du tissu source, les CSM-GW apparaissent comme étant nettement plus avantageuses que les CSM-MO. En conséquence, notre deuxième objectif a été d’évaluer l’action des CSM-GW dans un modèle porcin de péritonite afin de se rapprocher un peu plus près de la clinique humaine. Cette étude, menée en double aveugle et en présence continue d’un médecin réanimateur expérimenté, a permis de mettre en évidence que les CSM-GW, produites en grade clinique et utilisées juste après décongélation, étaient en mesure d’améliorer la survie, les paramètres hémodynamiques ainsi que les défaillances d’organes, selon un mécanisme d’action différent de celui rapporté par les études murines
Septic shock, equal to the myocardial infraction, is currently the tenth cause of death in the world. The pathophysiological complexity of this syndrome, with a simultaneous pro and anti- inflammatory state, results in the failure of conventional treatments. In this sense, research is focusing on innovative therapeutics agent, including mesenchymal stem cells (MSC). Indeed, murine studies of septic shock showed that MSC improve organ injuries, bacteremia and survival by notably a paracrine mechanism. However, MSC properties vary according to the source tissue, especially if they are derived from a fetal tissue (Wharton’s jelly (WJ), placenta amniotic fluid) or an adult tissue (bone marrow (BM), adipose tissue...). Our first objective was to compare, in a septic shock murine model, the effect of BM-MSC with that of WJ-MSC. Although some differences were observed, the same efficiency was demonstrated between these two sources. However, WJ-MSC present large advantages in comparison to BM-MSC due to their important proliferation capacities and potential quantities of umbilical cord donation. Consequently, our second objective was to investigate the effect of WJ-MSC administration in a relevant pig model of peritonitis in order to better mimic a clinical approach in humans. This study, conducted in double-blind and in presence of an experimented intensivist, showed that WJ-MSC produced in clinical grade and used immediately after thawing, improve survival, hemodynamic parameters and organ injuries by another action than that described in murine studies

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Reppel, Loïc. "Potentialité des cellules stromales de la gelée de Wharton en ingénierie du cartillage". Thesis, Université de Lorraine, 2014. http://www.theses.fr/2014LORR0164/document.

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Les cellules stromales/souches mésenchymateuses de la gelée de Wharton humaines (CSM-WJ) représentent une source abondante et intéressante de cellules souches pour des applications en ingénierie cellulaire et tissulaire. Leur origine fœtale leur confère des caractéristiques spécifiques par rapport aux cellules stromales/souches mésenchymateuses isolées à partir de moelle osseuse humaine (CSM-MO). Tout d'abord, le but de ce travail est d'optimiser les conditions de culture des CSM-GW pour leur utilisation clinique ultérieure. Nous nous concentrons sur l'influence de la concentration en oxygène lors de l'expansion en monocouche de P1 à P7 sur plusieurs paramètres permettant de caractériser les CSM. Les résultats obtenus sont comparés à ceux obtenus avec les CSM-MO. Notre travail a montré des différences entre les deux sources cellulaires en termes de prolifération et de différenciation adipocytaire. D’après nos résultats, l'hypoxie, au cours de l'expansion, est un paramètre important à prendre en compte en ce qui concerne la prolifération et le potentiel de différenciation chondrocytaire. L'influence des facteurs obstétricaux sur les caractéristiques des CSM-GW est également explorée. Cette étude se situant également dans le cadre de l’ingénierie tissulaire du cartilage, la seconde phase du projet consiste à induire la différenciation des cellules en chondrocytes en ensemençant ces dernières dans un biomatériau à base d’alginate et d’acide hyaluronique, et sur une cinétique de 28 jours. Les résultats obtenus sont comparés à ceux obtenus avec les CSM-MO. Après 4 semaines de culture, les CSM-GW sont capables de s'adapter à leur environnement et d’exprimer des gènes et des protéines matriciels spécifiques du cartilage tels que le collagène de type 2, qui se trouve plus exprimé après différenciation à partir des CSM-GW qu’à partir de CSM-MO
Mesenchymal Stromal/Stem Cells from human Wharton’s jelly (WJ-MSC) are an abundant and interesting source of stem cells for applications in cell and tissue engineering. Their fetal origin confers specific characteristics compared to Mesenchymal Stromal/Stem Cells isolated from human bone marrow (BM-MSC). First, the aim of this work is to optimize WJ-MSC culture conditions for their subsequent clinical use. We focus on the influence of oxygen concentration during monolayer expansion on several parameters to characterize MSC. The results are compared to those obtained with BM-MSC. Our work distinguishes WJ-MSC from BM-MSC in terms of proliferation and adipogenic differentiation. Considering our results, hypoxia during cell expansion is an important parameter to take into account regarding proliferation potential but also chondrogenic differentiation potential. The influence of obstetric factors on WJ-MSC characteristics is also explored. In cartilage tissue engineering context, the second phase of the project is to induce cell differentiation into chondrocytes by seeding them in Alginate/Hyaluronic Acid hydrogel scaffold, and during 28 days. The results obtained are compared to those obtained with BM-MSC. After 4 weeks of culture, WJ-MSC are able to adapt to their environment and express specific cartilage-Related genes and matrix proteins such as type 2 collagen, which is found more expressed after differentiation fromWJ-MSC, than from BM-MSC

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Yu, Hao. "Évaluation des caractéristiques des hydrogels d’alginate supplémentés en acide hyaluronique ou en hydroxyapatite lors de la différenciation des cellules souches mésenchymateuses issues de la gelée de Wharton". Thesis, Université de Lorraine, 2017. http://www.theses.fr/2017LORR0087/document.

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Dans le domaine de l'ingénierie du cartilage, les hydrogels à base d'alginate (Alg) et de cellules souches mésenchymateuses (CSM) sont utilisés comme biomatériaux pouvant être utilisés pour combler des lésions cartilagineuses plus ou moins profondes. Cependant, pour reproduire l’organisation zonale du cartilage, des biomatériaux multiphasiques sont nécessaires. Afin de guider la différenciation des CSM dans les différentes strates du biomatériau, sans apports de facteurs de croissance, des composants naturels du cartilage (acide hyaluronique, HA) ou de la matrice osseuse (hydroxyapatite, Hap) peuvent être ajoutés à l’alginate. L’objectif de ce travail de thèse consiste à analyser l’impact de la composition de biomatériaux à base d’alginate enrichi soit en HA soit en Hap sur le comportement des CSM. La première partie de notre travail à consister à évaluer le comportement des CSM issues de la gelée de Wharton dans ces hydrogels. Nos résultats mettent en évidence que les hydrogels d’Alg/Hap possèdent non seulement de meilleures propriétés mécaniques que les hydrogels Alg/HA et favorisent la viabilité des CSM ainsi que leur différenciation par rapport aux CSM ensemencées dans un hydrogel d’Alg/HA. La méthode de stérilisation du biomatériau représente une étape incontournable, dont on doit impérativement évaluer les multiples effets, en particulier pour ce qui touche au comportement des cellules, mais aussi au maintien de l’intégrité des propriétés physicochimiques de l'hydrogel. Ainsi, dans une seconde partie du travail, nous avons montré que le traitement de stérilisation par autoclave induisait un effet négatif sur les caractéristiques initiales de l'hydrogel à base d'alginate. Il ressort également de cette investigation sur les modes de stérilisation, que la stérilisation des hydrogels avec des UV est plus efficace et permet de préserver au mieux les propriétés spécifiques de l'hydrogel, notamment de l’Alg/HA. Enfin, dans une troisième partie de notre travail, nous avons évalué l’évolution des propriétés mécaniques au cours de la différenciation et l’impact de celles-ci sur la différenciation des CSM ainsi que sur leurs propriétés immunomodulatrices. À partir de ces résultats, nous avons montré que les caractéristiques physico-chimiques des hydrogels d’Alg/ha et Alg/hap influençaient non seulement le potentiel de différenciation des CSM-GW mais également la sécrétion des facteurs solubles impliqués dans l’immunomodulation. Ces propriétés physico-chimiques étant influencées dès le procédé de stérilisation, il est alors conseillé de les prendre en compte dans toutes les étapes de l’ingénierie tissulaire
In the field of cartilage engineering, alginate (Alg)-based hydrogels and mesenchymal stem cells (MSC) are widely used as raw biomaterials and stem cells which can be used to fill cartilage lesions of varying depth. However, to reproduce the zonal organization of articular cartilage, a graft multilayer is necessary. In order to guide the differentiation of MSCs in different strata of the biomaterials, without input of growth factors, natural cartilage components (hyaluronic acid, HA) or bone matrix (hydroxyapatite, Hap) can be added into the alginate. The aim of this work is to analyze the impact of the composition of alginate enriched either in HA or in Hap on the behavior of MSCs. The first part of our work is to evaluate the behavior of WJ-MSCs into these hydrogels. Our results have shown that Alg/ Hap hydrogels not only possess better mechanical properties than Alg/HA hydrogels, but also promote the viability of MSCs and their differentiation from MSC seeded into the Alg/HA hydrogel. The sterilization method of biomaterial is an essential step, the multiple effects of which must be evaluated, in particular as regards the behavior of the cells, but also to maintain the integrity of the physicochemical properties of hydrogel. Thus, in a second part of this work, we showed that the autoclave sterilization treatment induced a negative effect on the initial characteristics of alginate hydrogel. It is also apparent from this investigation of the sterilization modes that the sterilization of hydrogels with UV is more efficient and makes it possible to preserve the specific properties of the hydrogel as best as possible, in particular Alg/HA. Finally, in a third part of our work, we also evaluated the evolution of the mechanical properties during the differentiation and the impact of these on the differentiation of MSCs and their immunomodulatory properties. From these results, we have shown that the physico-chemical characteristics of Alg / ha and Alg/hap hydrogels influence not only the differentiation potential of WJ-MSC but also the secretion of soluble factors involved in immunomodulation. Since these physicochemical properties are influenced by the sterilization process, it is advisable to take them into account in all stages of tissue engineering

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Seshareddy, Kiran Babu. "Human Wharton's jelly cells-isolation and characterization in different growth conditions". Manhattan, Kan. : Kansas State University, 2008. http://hdl.handle.net/2097/1054.

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Badraiq, Heba Ghazi O. "Effects of maternal body weight on Wharton's Jelly mesenchymal stromal cells (pilot study)". Thesis, King's College London (University of London), 2017. https://kclpure.kcl.ac.uk/portal/en/theses/effects-of-maternal-body-weight-on-whartons-jelly-mesenchymal-stromal-cells-pilot-study(dac6be9c-1f9d-4c00-88dc-2a73ec4489b4).html.

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To investigate whether the maternal metabolic environment affects the DNA methylation of mesenchymal stromal/stem cells (MSCs) from umbilical cord (UC) Wharton’s Jelly (WJ), potentially rendering them unsuitable for clinical use in multiple recipients, a pilot study was conducted on fourteen UCs obtained post partum from healthy non-obese (BMI=19-25; n=7) and obese (BMI≥30; n=7) donors receiving elective Caesarean sections. The time of first WJ-MSCs outgrowth from UC explants was similar in samples from obese and non-obese donors. However, the cells from non-obese donors proliferated faster after 34 hours of culture than cells from obese donors. Differentiation into adipogenic, osteogenic and chondrogenic lineages was similar between obese and non-obese donor samples as demonstrated by tissue-specific staining and RT-PCR for lineage markers. However, WJ-MSCs from obese donors exhibited stronger immunosuppressive activity than those from non-obese donors. Genome-wide DNA methylation of triple-positive (CD73+CD90+CD105+) WJ-MSCs sorted from the first passage of a mixed population of cells was assessed. Samples from the obese and non-obese donors clustered separately, and 5,767 of the analysed CpG sites (1%) exhibited different methylation. Sixty-seven genes were observed with at least one CpG site with a methylation difference ≥0.2 in four or more obese donors. These 67 genes were further refined based on a list of polymorphic CpG sites and segmental duplications. In 18 of the 67 genes with a different CpG methylation pattern, the CpG sites were in non-polymorphic regions. However, two genes (DCAF6 and ZNF714) resided in segmentally duplicated regions. To determine whether methylation differences altered gene expression, the samples were analysed using a HumanHT-12 Expression BeadChip array and, of the 18 genes, only PNPLA7 was significantly affected at the mRNA level, which was confirmed independently by RT-PCR and Western blotting. Although the number of analysed donors was limited, the data suggest that an abnormal metabolic environment related to excessive body weight might alter the properties of WJ-MSCs used for cellular therapy.

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Libri sul tema "Wharton’s jelly"

Sarugaser, Rahul. Human umbilical cord Wharton's jelly as a source of mesenchymal progenitors capable of expressing a functional osteogenic phenotype in vitro. 2004.

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Capitoli di libri sul tema "Wharton’s jelly"

Bastawrous, Marina, Mibel M. Pabón, Sandra Acosta, Ike de la Peña, Diana Hernandez-Ontiveros, Meaghan Staples, Kazutaka Shinozuka et al. "Wharton’s Jelly Stem Cells". In Fetal Stem Cells in Regenerative Medicine, 257–76. New York, NY: Springer New York, 2016. http://dx.doi.org/10.1007/978-1-4939-3483-6_14.

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Walker, John T., Armand Keating e John E. Davies. "Stem Cells: Umbilical Cord/Wharton’s Jelly Derived". In Cell Engineering and Regeneration, 237–64. Cham: Springer International Publishing, 2020. http://dx.doi.org/10.1007/978-3-319-08831-0_10.

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Walker, John T., Armand Keating e John E. Davies. "Stem Cells: Umbilical Cord/Wharton’s Jelly Derived". In Cell Engineering and Regeneration, 1–28. Cham: Springer International Publishing, 2019. http://dx.doi.org/10.1007/978-3-319-37076-7_10-1.

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Converse, Gabriel L., Dandan Li, Eric E. Buse, Richard A. Hopkins e Omar S. Aljitawi. "Wharton’s Jelly Matrix Decellularization for Tissue Engineering Applications". In Methods in Molecular Biology, 25–33. New York, NY: Springer New York, 2017. http://dx.doi.org/10.1007/7651_2017_61.

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Baba, Kyoko, Yasuharu Yamazaki, Akira Takeda e Eiju Uchinuma. "Bone Regeneration Using Wharton’s Jelly Mesenchymal Stem Cells". In Perinatal Stem Cells, 299–311. New York, NY: Springer New York, 2014. http://dx.doi.org/10.1007/978-1-4939-1118-9_27.

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Weiss, Jeffrey N. "Wharton’s Jelly-derived Mesenchymal Stem Cells in Osteoarthritis". In Orthopedic Stem Cell Surgery, 115–21. Cham: Springer International Publishing, 2021. http://dx.doi.org/10.1007/978-3-030-73299-8_20.

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Weiss, M. L., Yelica López e K. R. McIntosh. "Wharton’s Jelly-Derived Mesenchymal Stromal Cells as Immunoregulatory Cells". In Human Fetal Tissue Transplantation, 87–105. London: Springer London, 2012. http://dx.doi.org/10.1007/978-1-4471-4171-6_7.

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Weiss, Jeffrey N. "Wharton’s Jelly Originated Mesenchymal Stem Cell in Gonarthrosis (WHAMKO)". In Orthopedic Stem Cell Surgery, 229–33. Cham: Springer International Publishing, 2021. http://dx.doi.org/10.1007/978-3-030-73299-8_45.

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Mellott, Adam J., Michael S. Detamore e Hinrich Staecker. "The Use of Human Wharton’s Jelly Cells for Cochlear Tissue Engineering". In Methods in Molecular Biology, 319–45. New York, NY: Springer New York, 2016. http://dx.doi.org/10.1007/978-1-4939-3615-1_19.

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Weiss, Jeffrey N. "Use of Wharton’s Jelly-Derived Mesenchymal Stem Cells for Knee Osteoarthrosis". In Orthopedic Stem Cell Surgery, 239–41. Cham: Springer International Publishing, 2021. http://dx.doi.org/10.1007/978-3-030-73299-8_47.

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Atti di convegni sul tema "Wharton’s jelly"

Kerékgyártó, Márta, Emőke Kiss-Tóth, László Barkai e Bertalan Fodor. "The Role of Wharton’s Jelly Mesenchymal Stem Cells in Diabetes Mellitus". In MultiScience - XXX. microCAD International Multidisciplinary Scientific Conference. University of Miskolc, 2016. http://dx.doi.org/10.26649/musci.2016.146.

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Dougherty, John, Emily Schaefer, Ryan Niemeier, Erin Koch, Craig Cady e Kalyani Nair. "Effect of Valproic Acid on Cell Proliferation of Wharton’s Jelly MSC in PCL Nanofiber Scaffolds". In ASME 2013 International Mechanical Engineering Congress and Exposition. American Society of Mechanical Engineers, 2013. http://dx.doi.org/10.1115/imece2013-65041.

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The field of tissue engineering and regenerative medicine is an interdisciplinary field that applies the principles of engineering and life sciences toward the development of biological substitutes that restore, maintain, or improve tissue function or a whole organ. The process involves seeding cells onto biocompatible scaffolds that temporarily act as a supporting structure for cells to attach and grow. Scaffolds for tissue regeneration must present a viable microenvironment for the living cells to adhere, proliferate, and exhibit the necessary tissue function. Electrospinning is an emerging area where polymeric fibers can be fabricated in the micro-nano scale. The flexibility of this process allows for including a wide array of synthetic and natural biocompatible polymers in the scaffold composition, inclusion of bioactive molecules (e.g. DNA, proteins) for enhancing therapeutic applications, and ability to control material and mechanical properties via the electrospinning process — all advantageous parameters that contribute to the promise of utilizing electrospun scaffolds in tissue repair. Biocompatible materials, such as polycaprolactone (PCL), have been used extensively to fabricate scaffolds using electrospinning technique, to study cell compatibility and to evaluate cell functionality for nerve tissue engineering applications. The objective of this study is to quantify the effects of the addition of valproic acid to PCL nanofiber scaffolds created through the electrospinning process with regards to cell proliferation. Valproic acid is a commonly used therapeutic drug for the treatment of epilepsy and bipolar disorder. To determine the effects of the presence of valproic acid (VA), Wharton’s jelly mesenchymal stem cells (MSC) are seeded to the two scaffolds. Wharton’s jelly MSC are multipotent adult stem cells present in the umbilical cord and drawn from their matrix [1,2,3]. These stem cells have renowned ability for use in cell therapy and organ regeneration. This study tests the hypothesis that the presence of valproic acid in PCL nanofiber scaffolds will enhance cell proliferation. Structural and morphological characterization of the scaffolds is also carried out. Fiber diameter and tensile properties of the scaffolds with and without valproic acid are also observed. Such studies will enable us to understand the effects of drugs such as valproic acid on stem cells and will aid in designing scaffolds for applications in nerve regeneration.

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Barlian, Anggraini, Hermawan Judawisastra, Ahmad Ridwan, Antonia Ratih e Noviana Vanawati. "Differentiation of human Wharton’s jelly mesenchymal stem cells on biomaterial-based scaffold for cartilage tissue engineering". In THE 4TH BIOMEDICAL ENGINEERING’S RECENT PROGRESS IN BIOMATERIALS, DRUGS DEVELOPMENT, HEALTH, AND MEDICAL DEVICES: Proceedings of the International Symposium of Biomedical Engineering (ISBE) 2019. AIP Publishing, 2019. http://dx.doi.org/10.1063/1.5139328.

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Hernando, A., D. H. A. Saputri, M. I. Tan e A. Barlian. "Directing the chondrogenic differentiation of human Wharton’s jelly mesenchymal stem cells using spider silk-based micropattern". In PROCEEDINGS OF THE INTERNATIONAL CONFERENCE AND SCHOOL ON PHYSICS IN MEDICINE AND BIOSYSTEM (ICSPMB): Physics Contribution in Medicine and Biomedical Applications. AIP Publishing, 2021. http://dx.doi.org/10.1063/5.0048014.

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Widowati, Wahyu, Teresa Liliana Wargasetia, Fanny Rahardja, Rimonta F. Gunanegara, Tri Handayani, Hanna Sari Widya Kusuma, Seila Arumwardana, Cahyaning Riski Wijayanti e Rizal Rizal. "Potential of Human Wharton’s Jelly Mesenchymal Stem Cells (hWJMSCs) Secretome for COVID-19 Adjuvant Therapy Candidate". In 2021 IEEE International Conference on Health, Instrumentation & Measurement, and Natural Sciences (InHeNce). IEEE, 2021. http://dx.doi.org/10.1109/inhence52833.2021.9537290.

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Sharma, Mayank, Michael Bellio, Jian Huang, Pingping Chen, Andreas Damianos, Augusto Scmidt, Shu Wu et al. "Wharton’s Jelly Mesenchymal Stem Cell-derived (WJ-MSC) exosomes for Severe Bronchopulmonary Dysplasia: A Dose-Finding Study". In AAP National Conference & Exhibition Meeting Abstracts. American Academy of Pediatrics, 2021. http://dx.doi.org/10.1542/peds.147.3_meetingabstract.752.

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Martin, John T., e Virginia L. Ferguson. "Regional Similarities in the Mechanical Properties of the Human Umbilical Artery". In ASME 2009 Summer Bioengineering Conference. American Society of Mechanical Engineers, 2009. http://dx.doi.org/10.1115/sbc2009-206800.

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The human umbilical cord (UC) bridges the blood flow gap between baby and mother, protecting the blood supply in a way that allows the fetus freedom to move within the amniotic sac. Once the blood supply has been oxygenated by the maternal blood pool via the placenta, the umbilical vein (UV) provides a transport pathway to the fetus. Two umbilical arteries (UA) return the blood supply to the pool to eliminate CO2 and other metabolic wastes [1]. The walls of the UA’s and UV are made up of an intima composed a single layer of large, elongated endothelial cells [2], and a media composed of randomly distributed smooth muscles cells, collagen, elastin, and ground substance. These vessels are unique in that their adventitia is absent and substituted by Wharton’s jelly, a mucoid connective tissue.

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Arrochman, Ferry, Eva Niamuzisilawati, Nurrachmat Mulianto e Indah Julianto. "The Effect of Conditioned Medium Derived Wharton’s Jelly Messenchymal Stem Cell for Diabetic Foot Ulcer: Preeliminary Study – Case Series". In The 23rd Regional Conference of Dermatology 2018. SCITEPRESS - Science and Technology Publications, 2018. http://dx.doi.org/10.5220/0008161305260528.

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Rizal, Rahimi Syaidah, Ziyan Muhammad Aqsha, Adella Josephin e Vidi Miranda Pakpahan. "Characterization, differentiation, and population doubling time of Wharton’s jelly mesenchymal stem cells (WJ-MSCs) in passage 5 and 8". In THE 5TH BIOMEDICAL ENGINEERING’S RECENT PROGRESS IN BIOMATERIALS, DRUGS DEVELOPMENT, AND MEDICAL DEVICES: Proceedings of the 5th International Symposium of Biomedical Engineering (ISBE) 2020. AIP Publishing, 2021. http://dx.doi.org/10.1063/5.0047340.

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Barlian, A., H. Judawisastra, A. Ridwan, A. R. Wahyuni e M. E. Lingga. "Attachment, spreading, and proliferation of Wharton’s jelly mesenchymal stem cells on scaffold combination of silk fibroin and Argiope appensa silk spidroin". In PROCEEDINGS OF THE INTERNATIONAL CONFERENCE AND SCHOOL ON PHYSICS IN MEDICINE AND BIOSYSTEM (ICSPMB): Physics Contribution in Medicine and Biomedical Applications. AIP Publishing, 2021. http://dx.doi.org/10.1063/5.0048163.

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