Bibliografie tematiche / Whole genome amplification / Articoli di riviste
Segui questo link per vedere altri tipi di pubblicazioni sul tema: Whole genome amplification.

Articoli di riviste sul tema "Whole genome amplification"

Autore: Grafiati
Pubblicato: 4 giugno 2021
Ultima modifica: 9 marzo 2023

Cita una fonte nei formati APA, MLA, Chicago, Harvard e in molti altri stili

Scegli il tipo di fonte:

Vedi i top-50 articoli di riviste per l'attività di ricerca sul tema "Whole genome amplification".

Accanto a ogni fonte nell'elenco di riferimenti c'è un pulsante "Aggiungi alla bibliografia". Premilo e genereremo automaticamente la citazione bibliografica dell'opera scelta nello stile citazionale di cui hai bisogno: APA, MLA, Harvard, Chicago, Vancouver ecc.

Puoi anche scaricare il testo completo della pubblicazione scientifica nel formato .pdf e leggere online l'abstract (il sommario) dell'opera se è presente nei metadati.

Vedi gli articoli di riviste di molte aree scientifiche e compila una bibliografia corretta.

1

Arneson, N., S. Hughes, R. Houlston, and S. Done. "GenomePlex Whole-Genome Amplification." Cold Spring Harbor Protocols 2008, no. 2 (January 1, 2008): pdb.prot4920. http://dx.doi.org/10.1101/pdb.prot4920.

Testo completo
Gli stili APA, Harvard, Vancouver, ISO e altri
2

Li, Ying, Hyun-Jin Kim, Chunyang Zheng, Wing Huen A. Chow, Jeonghwa Lim, Brendan Keenan, Xiaojing Pan, Bertrand Lemieux, and Huimin Kong. "Primase-based whole genome amplification." Nucleic Acids Research 36, no. 13 (June 17, 2008): e79-e79. http://dx.doi.org/10.1093/nar/gkn377.

Testo completo
Gli stili APA, Harvard, Vancouver, ISO e altri
3

Seong, Ji-Yeong, Young-Jun Ko, Hyeon-Koon Myeong, and Se-Wook Oh. "Development of a Rapid Foodborne-pathogen-detection Method Involving Whole-genome Amplification." Korean Journal of Food Science and Technology 48, no. 2 (April 30, 2016): 128–32. http://dx.doi.org/10.9721/kjfst.2016.48.2.128.

Testo completo
Gli stili APA, Harvard, Vancouver, ISO e altri
4

Sun, Fengzhu, and Michael S. Waterman. "Whole Genome Amplification and Branching Processes." Advances in Applied Probability 29, no. 3 (September 1997): 629–68. http://dx.doi.org/10.2307/1428080.

Testo completo
Abstract (sommario):
Whole genome amplification is important for multipoint mapping by sperm or oocyte typing and genetic disease diagnosis. Polymerase chain reaction is not suitable for amplifying long DNA sequences. This paper studies a new technique, designated PEP-primer-extension-preamplification, for amplifying long DNA sequences using the theory of branching processes. A mathematical model for PEP is constructed and a closed formula for the expected target yield is obtained. A central limit theorem and a strong law of large numbers for the number of kth generation target sequences are proved.
Gli stili APA, Harvard, Vancouver, ISO e altri
5

Höckner, M., M. Erdel, A. Spreiz, G. Utermann, and D. Kotzot. "Whole Genome Amplification from Microdissected Chromosomes." Cytogenetic and Genome Research 125, no. 2 (2009): 98–102. http://dx.doi.org/10.1159/000227832.

Testo completo
Gli stili APA, Harvard, Vancouver, ISO e altri
6

Hawkins, Trevor L., John C. Detter, and Paul M. Richardson. "Whole genome amplification — applications and advances." Current Opinion in Biotechnology 13, no. 1 (February 2002): 65–67. http://dx.doi.org/10.1016/s0958-1669(02)00286-0.

Testo completo
Gli stili APA, Harvard, Vancouver, ISO e altri
7

Sun, Fengzhu, and Michael S. Waterman. "Whole Genome Amplification and Branching Processes." Advances in Applied Probability 29, no. 03 (September 1997): 629–68. http://dx.doi.org/10.1017/s0001867800028287.

Testo completo
Abstract (sommario):
Whole genome amplification is important for multipoint mapping by sperm or oocyte typing and genetic disease diagnosis. Polymerase chain reaction is not suitable for amplifying long DNA sequences. This paper studies a new technique, designated PEP-primer-extension-preamplification, for amplifying long DNA sequences using the theory of branching processes. A mathematical model for PEP is constructed and a closed formula for the expected target yield is obtained. A central limit theorem and a strong law of large numbers for the number of kth generation target sequences are proved.
Gli stili APA, Harvard, Vancouver, ISO e altri
8

Bassaganyas, Laia, George Freedman, Dedeepya Vaka, Eunice Wan, Richard Lao, Flavia Chen, Mark Kvale, Robert J. Currier, Jennifer M. Puck, and Pui-Yan Kwok. "Whole exome and whole genome sequencing with dried blood spot DNA without whole genome amplification." Human Mutation 39, no. 1 (November 6, 2017): 167–71. http://dx.doi.org/10.1002/humu.23356.

Testo completo
Gli stili APA, Harvard, Vancouver, ISO e altri
9

Zheng, Ying-ming, Ning Wang, Lei Li, and Fan Jin. "Whole genome amplification in preimplantation genetic diagnosis." Journal of Zhejiang University SCIENCE B 12, no. 1 (January 2011): 1–11. http://dx.doi.org/10.1631/jzus.b1000196.

Testo completo
Gli stili APA, Harvard, Vancouver, ISO e altri
10

Burtt, N. P. "Whole-Genome Amplification Using 29 DNA Polymerase." Cold Spring Harbor Protocols 2011, no. 1 (January 1, 2011): pdb.prot5552. http://dx.doi.org/10.1101/pdb.prot5552.

Testo completo
Gli stili APA, Harvard, Vancouver, ISO e altri
11

Garcia-Chapa, Meritxell, Assumpció Batlle, Djaouida Rekab, Michel Ruiz Rosquete, and Giuseppe Firrao. "PCR-mediated whole genome amplification of phytoplasmas." Journal of Microbiological Methods 56, no. 2 (February 2004): 231–42. http://dx.doi.org/10.1016/j.mimet.2003.10.010.

Testo completo
Gli stili APA, Harvard, Vancouver, ISO e altri
12

Lao, Kaiqin, Nan Lan Xu, and Neil A. Straus. "Whole genome amplification using single-primer PCR." Biotechnology Journal 3, no. 3 (March 2008): 378–82. http://dx.doi.org/10.1002/biot.200700253.

Testo completo
Gli stili APA, Harvard, Vancouver, ISO e altri
13

Tu, Jing, Yi Qiao, Yuhan Luo, Naiyun Long, and Zuhong Lu. "Quantifying genome DNA during whole-genome amplification via quantitative real-time multiple displacement amplification." RSC Advances 11, no. 8 (2021): 4617–21. http://dx.doi.org/10.1039/d0ra09021b.

Testo completo
Gli stili APA, Harvard, Vancouver, ISO e altri
14

Sujayanont, Patcharawan, Kwanrutai Chininmanu, Boonrat Tassaneetrithep, Nattaya Tangthawornchaikul, Prida Malasit, and Prapat Suriyaphol. "Comparison of phi29-based whole genome amplification and whole transcriptome amplification in dengue virus." Journal of Virological Methods 195 (January 2014): 141–47. http://dx.doi.org/10.1016/j.jviromet.2013.10.005.

Testo completo
Gli stili APA, Harvard, Vancouver, ISO e altri
15

Tay, Andy, Rajan P. Kulkarni, Armin Karimi, and Dino Di Carlo. "Research highlights: enhancing whole genome amplification using compartmentalization." Lab on a Chip 15, no. 23 (2015): 4379–82. http://dx.doi.org/10.1039/c5lc90117k.

Testo completo
Gli stili APA, Harvard, Vancouver, ISO e altri
16

Raz, Ofir, Liming Tao, Tamir Biezuner, Tzipy Marx, Yaara Neumeier, Narek Tumanyan, and Ehud Shapiro. "Whole-Genome Amplification—Surveying Yield, Reproducibility, and Heterozygous Balance, Reported by STR-Targeting MIPs." International Journal of Molecular Sciences 23, no. 11 (May 31, 2022): 6161. http://dx.doi.org/10.3390/ijms23116161.

Testo completo
Abstract (sommario):
Whole-genome amplification is a crucial first step in nearly all single-cell genomic analyses, with the following steps focused on its products. Bias and variance caused by the whole-genome amplification process add numerous challenges to the world of single-cell genomics. Short tandem repeats are sensitive genomic markers used widely in population genetics, forensics, and retrospective lineage tracing. A previous evaluation of common whole-genome amplification targeting ~1000 non-autosomal short tandem repeat loci is extended here to ~12,000 loci across the entire genome via duplex molecular
Gli stili APA, Harvard, Vancouver, ISO e altri
17

Nyaku, S. T., V. R. Sripathi, K. Lawrence, and G. Sharma. "Characterizing Repeats in Two Whole-Genome Amplification Methods in the Reniform Nematode Genome." International Journal of Genomics 2021 (March 6, 2021): 1–8. http://dx.doi.org/10.1155/2021/5532885.

Testo completo
Abstract (sommario):
One of the major problems in the U.S. and global cotton production is the damage caused by the reniform nematode, Rotylenchulus reniformis. Amplification of DNA from single nematodes for further molecular analysis can be challenging sometimes. In this research, two whole-genome amplification (WGA) methods were evaluated for their efficiencies in DNA amplification from a single reniform nematode. The WGA was carried out using both REPLI-g Mini and Midi kits, and the GenomePlex single cell whole-genome amplification kit. Sequence analysis produced 4 Mb and 12 Mb of genomic sequences for the reni
Gli stili APA, Harvard, Vancouver, ISO e altri
18

Karere, Genesio M., Leslie A. Lyons, and Lutz Froenicke. "Enhancing radiation hybrid mapping through whole genome amplification." Hereditas 147, no. 2 (May 4, 2010): 103–12. http://dx.doi.org/10.1111/j.1601-5223.2010.02166.x.

Testo completo
Gli stili APA, Harvard, Vancouver, ISO e altri
19

Hosono, S. "Unbiased Whole-Genome Amplification Directly From Clinical Samples." Genome Research 13, no. 5 (April 14, 2003): 954–64. http://dx.doi.org/10.1101/gr.816903.

Testo completo
Gli stili APA, Harvard, Vancouver, ISO e altri
20

Kaper, F., S. Swamy, B. Klotzle, S. Munchel, J. Cottrell, M. Bibikova, H. Y. Chuang, et al. "Whole-genome haplotyping by dilution, amplification, and sequencing." Proceedings of the National Academy of Sciences 110, no. 14 (March 18, 2013): 5552–57. http://dx.doi.org/10.1073/pnas.1218696110.

Testo completo
Gli stili APA, Harvard, Vancouver, ISO e altri
21

Lack, Justin B., Lawrence J. Weider, and Punidan D. Jeyasingh. "Whole genome amplification and sequencing of aDaphniaresting egg." Molecular Ecology Resources 18, no. 1 (October 14, 2017): 118–27. http://dx.doi.org/10.1111/1755-0998.12720.

Testo completo
Gli stili APA, Harvard, Vancouver, ISO e altri
22

Kwok, Pui-Yan. "Making ‘random amplification’ predictable in whole genome analysis." Trends in Biotechnology 20, no. 10 (October 2002): 411–12. http://dx.doi.org/10.1016/s0167-7799(02)02034-6.

Testo completo
Gli stili APA, Harvard, Vancouver, ISO e altri
23

Keller, Martin. "Whole genome amplification: watch out for the bias!" Environmental Microbiology 16, no. 3 (March 2014): 611. http://dx.doi.org/10.1111/1462-2920.12401.

Testo completo
Gli stili APA, Harvard, Vancouver, ISO e altri
24

Spits, Claudia, Cédric Le Caignec, Martine De Rycke, Lindsey Van Haute, André Van Steirteghem, Inge Liebaers, and Karen Sermon. "Whole-genome multiple displacement amplification from single cells." Nature Protocols 1, no. 4 (November 2006): 1965–70. http://dx.doi.org/10.1038/nprot.2006.326.

Testo completo
Gli stili APA, Harvard, Vancouver, ISO e altri
25

Chen, Lin, Andreas Manz, and Philip J. R. Day. "Whole genome amplification on poly(dimethylsiloxane) microchip array." Analytical Biochemistry 372, no. 1 (January 2008): 128–30. http://dx.doi.org/10.1016/j.ab.2007.07.036.

Testo completo
Gli stili APA, Harvard, Vancouver, ISO e altri
26

Balogh, M. K., C. Børsting, P. Sánchez Diz, C. Thacker, D. Syndercombe-Court, A. Carracedo, N. Morling, and P. M. Schneider. "Application of whole genome amplification for forensic analysis." International Congress Series 1288 (April 2006): 725–27. http://dx.doi.org/10.1016/j.ics.2005.12.017.

Testo completo
Gli stili APA, Harvard, Vancouver, ISO e altri
27

Abulencia, Carl B., Denise L. Wyborski, Joseph A. Garcia, Mircea Podar, Wenqiong Chen, Sherman H. Chang, Hwai W. Chang, et al. "Environmental Whole-Genome Amplification To Access Microbial Populations in Contaminated Sediments." Applied and Environmental Microbiology 72, no. 5 (May 2006): 3291–301. http://dx.doi.org/10.1128/aem.72.5.3291-3301.2006.

Testo completo
Abstract (sommario):
ABSTRACT Low-biomass samples from nitrate and heavy metal contaminated soils yield DNA amounts that have limited use for direct, native analysis and screening. Multiple displacement amplification (MDA) using φ29 DNA polymerase was used to amplify whole genomes from environmental, contaminated, subsurface sediments. By first amplifying the genomic DNA (gDNA), biodiversity analysis and gDNA library construction of microbes found in contaminated soils were made possible. The MDA method was validated by analyzing amplified genome coverage from approximately five Escherichia coli cells, resulting i
Gli stili APA, Harvard, Vancouver, ISO e altri
28

Liu, C. L., B. E. Bernstein, and S. L. Schreiber. "Whole Genome Amplification by T7-Based Linear Amplification of DNA (TLAD): Overview." Cold Spring Harbor Protocols 2008, no. 6 (May 1, 2008): pdb.top42. http://dx.doi.org/10.1101/pdb.top42.

Testo completo
Gli stili APA, Harvard, Vancouver, ISO e altri
29

Foster, Simon J., and Brendon J. Monahan. "Whole genome amplification from filamentous fungi using Phi29-mediated multiple displacement amplification." Fungal Genetics and Biology 42, no. 5 (May 2005): 367–75. http://dx.doi.org/10.1016/j.fgb.2005.01.013.

Testo completo
Gli stili APA, Harvard, Vancouver, ISO e altri
30

Shortt, Jonathan A., Daren C. Card, Drew R. Schield, Yang Liu, Bo Zhong, Todd A. Castoe, Elizabeth J. Carlton, and David D. Pollock. "Whole Genome Amplification and Reduced-Representation Genome Sequencing of Schistosoma japonicum Miracidia." PLOS Neglected Tropical Diseases 11, no. 1 (January 20, 2017): e0005292. http://dx.doi.org/10.1371/journal.pntd.0005292.

Testo completo
Gli stili APA, Harvard, Vancouver, ISO e altri
31

Kwon, Young M., and Mandy M. Cox. "Improved efficacy of whole genome amplification from bacterial cells." BioTechniques 37, no. 1 (July 2004): 40–44. http://dx.doi.org/10.2144/04371bm03.

Testo completo
Gli stili APA, Harvard, Vancouver, ISO e altri
32

Hall, Eric, Samuel Kim, Visham Appadoo, and Richard Zare. "Lysis of a Single Cyanobacterium for Whole Genome Amplification." Micromachines 4, no. 3 (August 21, 2013): 321–32. http://dx.doi.org/10.3390/mi4030321.

Testo completo
Gli stili APA, Harvard, Vancouver, ISO e altri
33

Lepere, Cécile, Mikihide Demura, Masanobu Kawachi, Sarah Romac, Ian Probert, and Daniel Vaulot. "Whole-genome amplification (WGA) of marine photosynthetic eukaryote populations." FEMS Microbiology Ecology 76, no. 3 (March 16, 2011): 513–23. http://dx.doi.org/10.1111/j.1574-6941.2011.01072.x.

Testo completo
Gli stili APA, Harvard, Vancouver, ISO e altri
34

Brooks, R., R. J. Rose, M. B. Sheahan, and S. Kurdyukov. "Whole genome methylation scanning based on phi29 polymerase amplification." Biochemistry (Moscow) 76, no. 9 (September 2011): 999–1002. http://dx.doi.org/10.1134/s0006297911090021.

Testo completo
Gli stili APA, Harvard, Vancouver, ISO e altri
35

Sun, Guangyun, Ritesh Kaushal, Prodipto Pal, Michael Wolujewicz, Diane Smelser, Hong Cheng, Mei Lu, Ranajit Chakraborty, Li Jin, and Ranjan Deka. "Whole-genome amplification: relative efficiencies of the current methods." Legal Medicine 7, no. 5 (October 2005): 279–86. http://dx.doi.org/10.1016/j.legalmed.2005.05.001.

Testo completo
Gli stili APA, Harvard, Vancouver, ISO e altri
36

Yu, Zhilong, Sijia Lu, and Yanyi Huang. "Microfluidic Whole Genome Amplification Device for Single Cell Sequencing." Analytical Chemistry 86, no. 19 (September 22, 2014): 9386–90. http://dx.doi.org/10.1021/ac5032176.

Testo completo
Gli stili APA, Harvard, Vancouver, ISO e altri
37

Bergen, A. W. "Effects of Electron-Beam Irradiation on Whole Genome Amplification." Cancer Epidemiology Biomarkers & Prevention 14, no. 4 (April 1, 2005): 1016–19. http://dx.doi.org/10.1158/1055-9965.epi-04-0686.

Testo completo
Gli stili APA, Harvard, Vancouver, ISO e altri
38

Fu, Yusi, Chunmei Li, Sijia Lu, Wenxiong Zhou, Fuchou Tang, X. Sunney Xie, and Yanyi Huang. "Uniform and accurate single-cell sequencing based on emulsion whole-genome amplification." Proceedings of the National Academy of Sciences 112, no. 38 (September 4, 2015): 11923–28. http://dx.doi.org/10.1073/pnas.1513988112.

Testo completo
Abstract (sommario):
Whole-genome amplification (WGA) for next-generation sequencing has seen wide applications in biology and medicine when characterization of the genome of a single cell is required. High uniformity and fidelity of WGA is needed to accurately determine genomic variations, such as copy number variations (CNVs) and single-nucleotide variations (SNVs). Prevailing WGA methods have been limited by fluctuation of the amplification yield along the genome, as well as false-positive and -negative errors for SNV identification. Here, we report emulsion WGA (eWGA) to overcome these problems. We divide sing
Gli stili APA, Harvard, Vancouver, ISO e altri
39

Buffart, Tineke E., Marianne Tijssen, Thijs Krugers, Beatriz Carvalho, Serge J. Smeets, Ruud H. Brakenhoff, Heike Grabsch, Gerrit A. Meijer, Henry B. Sadowski, and Bauke Ylstra. "DNA Quality Assessment for Array CGH by Isothermal Whole Genome Amplification." Analytical Cellular Pathology 29, no. 4 (January 1, 2007): 351–59. http://dx.doi.org/10.1155/2007/709290.

Testo completo
Abstract (sommario):
Background: Array Comparative Genomic Hybridization (array CGH) is increasingly applied on DNA obtained from formalin-fixed paraffin-embedded (FFPE) tissue, but in a proportion of cases this type of DNA is unsuitable. Due to the high experimental costs of array CGH and unreliable methods for DNA quality testing, better prediction methods are needed. The aim of this study was to accurately determine the quality of FFPE DNA input in order to predict quality of array CGH outcome. Material and Methods: DNA quality was assessed by isothermal amplification and compared to array CGH quality on 59 FFP
Gli stili APA, Harvard, Vancouver, ISO e altri
40

Bourrier, Chantal, Jean-Yves Pierga, Laura Xuereb, Hélène Salaun, Charlotte Proudhon, Michael R. Speicher, Jelena Belic, Ellen Heitzer, Brian Paul Lockhart, and Nolwen Guigal-Stephan. "Shallow Whole-Genome Sequencing from Plasma Identifies FGFR1 Amplified Breast Cancers and Predicts Overall Survival." Cancers 12, no. 6 (June 6, 2020): 1481. http://dx.doi.org/10.3390/cancers12061481.

Testo completo
Abstract (sommario):
Background: Focal amplification of fibroblast growth factor receptor 1 (FGFR1) defines a subgroup of breast cancers with poor prognosis and high risk of recurrence. We sought to demonstrate the potential of circulating cell-free DNA (cfDNA) analysis to evaluate FGFR1 copy numbers from a cohort of 100 metastatic breast cancer (mBC) patients. Methods: Formalin-fixed paraffin-embedded (FFPE) tissue samples were screened for FGFR1 amplification by FISH, and positive cases were confirmed with a microarray platform (OncoscanTM). Subsequently, cfDNA was evaluated by two approaches, i.e., mFAST-SeqS a
Gli stili APA, Harvard, Vancouver, ISO e altri
41

Wang, Jian, Joy D. Van Nostrand, Liyou Wu, Zhili He, Guanghe Li, and Jizhong Zhou. "Microarray-Based Evaluation of Whole-Community Genome DNA Amplification Methods." Applied and Environmental Microbiology 77, no. 12 (April 15, 2011): 4241–45. http://dx.doi.org/10.1128/aem.01834-10.

Testo completo
Abstract (sommario):
ABSTRACTThree whole-community genome amplification methods,Bst, REPLI-g, and Templiphi, were evaluated using a microarray-based approach. The amplification biases of all methods were <3-fold. For pure-culture DNA, REPLI-g and Templiphi showed less bias thanBst. For community DNA, REPLI-g showed the least bias and highest number of genes, whileBsthad the highest success rate and was suitable for low-quality DNA.
Gli stili APA, Harvard, Vancouver, ISO e altri
42

Luka, Martha M., Everlyn Kamau, Zaydah R. de Laurent, John Mwita Morobe, Leonard K. Alii, D. James Nokes, and Charles N. Agoti. "Whole genome sequencing of two human rhinovirus A types (A101 and A15) detected in Kenya, 2016-2018." Wellcome Open Research 6 (September 23, 2021): 178. http://dx.doi.org/10.12688/wellcomeopenres.16911.2.

Testo completo
Abstract (sommario):
Background: Virus genome sequencing is increasingly utilized in epidemiological surveillance. Genomic data allows comprehensive evaluation of underlying viral diversity and epidemiology to inform control. For human rhinovirus (HRV), genomic amplification and sequencing is challenging due to numerous types, high genetic diversity and inadequate reference sequences. Methods: We developed a tiled amplicon type-specific protocol for genome amplification and sequencing on the Illumina MiSeq platform of two HRV types, A15 and A101. We then assessed added value in analyzing whole genomes relative to
Gli stili APA, Harvard, Vancouver, ISO e altri
43

Luka, Martha M., Everlyn Kamau, Zaydah R. de Laurent, John Mwita Morobe, Leonard K. Alii, D. James Nokes, and Charles N. Agoti. "Whole genome sequencing of two human rhinovirus A types (A101 and A15) detected in Kenya, 2016-2018." Wellcome Open Research 6 (July 8, 2021): 178. http://dx.doi.org/10.12688/wellcomeopenres.16911.1.

Testo completo
Abstract (sommario):
Background: Virus genome sequencing is increasingly utilized in epidemiological surveillance. Genomic data allows comprehensive evaluation of underlying viral diversity and epidemiology to inform control. For human rhinovirus (HRV), genomic amplification and sequencing is challenging due to numerous types, high genetic diversity and inadequate reference sequences. Methods: We developed a tiled amplicon type-specific protocol for genome amplification and sequencing on the Illumina MiSeq platform of two HRV types, A15 and A101. We then assessed added value in analyzing whole genomes relative to
Gli stili APA, Harvard, Vancouver, ISO e altri
44

Pappoe-Ashong, Prince Jonathan, and Kwamena Sagoe. "Optimization of a Polymerase Chain Reaction System for Whole Genome Amplification of Human Immunodeficiency Virus Type 2 (HIV-2)." Open Forum Infectious Diseases 4, suppl_1 (2017): S359. http://dx.doi.org/10.1093/ofid/ofx163.871.

Testo completo
Abstract (sommario):
Abstract Background In this study, attempts were made to amplify the whole genome of HIV-2 using polymerase chain reaction (PCR) from viral RNA and proviral DNA extracted from archived human plasma and whole blood, respectively. The aim this study is to develop a PCR system that can be used to amplify the entire genome of all known subtypes and recombinant forms of HIV-2 Methods Proviral DNA and viral RNA were extracted from archived human whole blood and plasma using Zymo Research Viral DNA kit and Zymo Research Viral nucleic acids kit, respectively. Primers that target the conserved sites of
Gli stili APA, Harvard, Vancouver, ISO e altri
45

Paunio, T., I. Reima, and A. C. Syvänen. "Preimplantation diagnosis by whole-genome amplification, PCR amplification, and solid-phase minisequencing of blastomere DNA." Clinical Chemistry 42, no. 9 (September 1, 1996): 1382–90. http://dx.doi.org/10.1093/clinchem/42.9.1382.

Testo completo
Abstract (sommario):
Abstract We have developed a new method for preimplantation diagnosis of inherited diseases. Our procedure for the identification of point mutations in single cells combines whole-genome amplification using 15-mer random primers (primer extension preamplification, PEP) with a single locus-specific PCR amplification, followed by detection of the mutation by solid-phase minisequencing. The procedure was evaluated by detecting three disease-causing mutations and seven polymorphic nucleotides located on different human chromosomes from single granuloma and blastomere cells. The correct genotype of
Gli stili APA, Harvard, Vancouver, ISO e altri
46

Gardner, Shea N., Crystal J. Jaing, Maher M. Elsheikh, José Peña, David A. Hysom, and Monica K. Borucki. "Multiplex Degenerate Primer Design for Targeted Whole Genome Amplification of Many Viral Genomes." Advances in Bioinformatics 2014 (August 3, 2014): 1–8. http://dx.doi.org/10.1155/2014/101894.

Testo completo
Abstract (sommario):
Background. Targeted enrichment improves coverage of highly mutable viruses at low concentration in complex samples. Degenerate primers that anneal to conserved regions can facilitate amplification of divergent, low concentration variants, even when the strain present is unknown. Results. A tool for designing multiplex sets of degenerate sequencing primers to tile overlapping amplicons across multiple whole genomes is described. The new script, run_tiled_primers, is part of the PriMux software. Primers were designed for each segment of South American hemorrhagic fever viruses, tick-borne encep
Gli stili APA, Harvard, Vancouver, ISO e altri
47

Brochet, Mathieu, Elisabeth Couvé, Mohamed Zouine, Claire Poyart, and Philippe Glaser. "A Naturally Occurring Gene Amplification Leading to Sulfonamide and Trimethoprim Resistance in Streptococcus agalactiae." Journal of Bacteriology 190, no. 2 (November 16, 2007): 672–80. http://dx.doi.org/10.1128/jb.01357-07.

Testo completo
Abstract (sommario):
ABSTRACT Gene amplifications have been detected as a transitory phenomenon in bacterial cultures. They are predicted to contribute to rapid adaptation by simultaneously increasing the expression of genes clustered on the chromosome. However, genome amplifications have rarely been described in natural isolates. Through DNA array analysis, we have identified two Streptococcus agalactiae strains carrying tandem genome amplifications: a fourfold amplification of 13.5 kb and a duplication of 92 kb. Both amplifications were located close to the terminus of replication and originated independently fr
Gli stili APA, Harvard, Vancouver, ISO e altri
48

Wu, Liyou, Xueduan Liu, Christopher W. Schadt, and Jizhong Zhou. "Microarray-Based Analysis of Subnanogram Quantities of Microbial Community DNAs by Using Whole-Community Genome Amplification." Applied and Environmental Microbiology 72, no. 7 (July 2006): 4931–41. http://dx.doi.org/10.1128/aem.02738-05.

Testo completo
Abstract (sommario):
ABSTRACT Microarray technology provides the opportunity to identify thousands of microbial genes or populations simultaneously, but low microbial biomass often prevents application of this technology to many natural microbial communities. We developed a whole-community genome amplification-assisted microarray detection approach based on multiple displacement amplification. The representativeness of amplification was evaluated using several types of microarrays and quantitative indexes. Representative detection of individual genes or genomes was obtained with 1 to 100 ng DNA from individual or
Gli stili APA, Harvard, Vancouver, ISO e altri
49

Sobol, Morgan S., and Anne-Kristin Kaster. "Back to Basics: A Simplified Improvement to Multiple Displacement Amplification for Microbial Single-Cell Genomics." International Journal of Molecular Sciences 24, no. 5 (February 21, 2023): 4270. http://dx.doi.org/10.3390/ijms24054270.

Testo completo
Abstract (sommario):
Microbial single-cell genomics (SCG) provides access to the genomes of rare and uncultured microorganisms and is a complementary method to metagenomics. Due to the femtogram-levels of DNA in a single microbial cell, sequencing the genome requires whole genome amplification (WGA) as a preliminary step. However, the most common WGA method, multiple displacement amplification (MDA), is known to be costly and biased against specific genomic regions, preventing high-throughput applications and resulting in uneven genome coverage. Thus, obtaining high-quality genomes from many taxa, especially minor
Gli stili APA, Harvard, Vancouver, ISO e altri
50

Rouse, Nicholas A., Michael F. Nagy, Paul Smith, and Peter L. Nagy. "ASSESSMENT OF BIOSKRYB WHOLE GENOME AMPLIFICATION METHODOLOGY FOR GENOME-WIDE ASSESSMENT FOR SNPs AND INDELS USING ILLUMINA WHOLE GENOME SEQUENCING." Fertility and Sterility 116, no. 3 (September 2021): e391. http://dx.doi.org/10.1016/j.fertnstert.2021.07.1046.

Testo completo
Gli stili APA, Harvard, Vancouver, ISO e altri
Ti possono interessare anche gli elenchi di altri tipi di fonti sul tema "Whole genome amplification":
Tesi
Offriamo sconti su tutti i piani premium per gli autori le cui opere sono incluse in raccolte letterarie tematiche. Contattaci per ottenere un codice promozionale unico!

Vai alla bibliografia