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Artykuły w czasopismach na temat „Corneal transplantation”

Autor: Grafiati

Data publikacji: 4 czerwca 2021

Data aktualizacji: 25 lipca 2025

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1

Nakagawa, Hayate, Tomas Blanco, Francesca Kahale, Rohan Bir Singh, Thomas H. Dohlman, and Reza Dana. "Novel adaptation of a running suture technique in a mouse model of corneal transplantation." Journal of Biological Methods 8, no. 4 (2021): 1. http://dx.doi.org/10.14440/jbm.2021.373.

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Several murine models of corneal transplantation have been developed over the years to study the immunopathological processes that lead to the failure of grafted corneas. In all of them, the classic eight interrupted sutures technique is utilized for transplanting the donor cornea on the host bed. However, in clinical practice, a single continuous suture with a single knot is generally performed for corneal transplantation. Here, we describe the adaptation of the single continuous suture technique in a mouse model of corneal transplantation.

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Salabarria, Ann-Charlott, Manuel Koch, Alfrun Schönberg, et al. "Topical VEGF-C/D Inhibition Prevents Lymphatic Vessel Ingrowth into Cornea but Does Not Improve Corneal Graft Survival." Journal of Clinical Medicine 9, no. 5 (2020): 1270. http://dx.doi.org/10.3390/jcm9051270.

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Vascular endothelial growth factor-C/D (VEGF-C/D) regulates lymphangiogenesis. Ingrowth of lymphatic vessels is negatively associated with corneal transplantation success. In this study, we therefore analyzed the effect local blockade of VEGF-C/D has on inflamed corneas. We used the murine model of suture-induced neovascularization and subsequent high-risk corneal transplantation. Mice were treated with a VEGF-C/D trap prior to transplantation. Topical inhibition of VEGF-C/D significantly reduced lymphatic vessel ingrowth, but increased Macrophage numbers in the cornea. Furthermore, corneal tr

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Procházková, Alexandra, Martina Poláchová, Jakub Dítě, Magdaléna Netuková, and Pavel Studený. "Chemical, Physical, and Biological Corneal Decellularization Methods: A Review of Literature." Journal of Ophthalmology 2024 (March 25, 2024): 1–17. http://dx.doi.org/10.1155/2024/1191462.

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The cornea is one of the most commonly transplanted tissues worldwide. It is used to restore vision when severe visual impairment or blindness occurs in patients with corneal diseases or after trauma. Due to the global shortage of healthy donor corneas, decellularized corneal tissue has significant potential as an alternative to corneal transplantation. It preserves the native and biological ultrastructure of the cornea and, therefore, represents the most promising scaffold. This article discusses different methods of corneal decellularization based on the current literature. We searched PubMe

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Sakowska, Justyna, Paulina Glasner, Maciej Zieliński, Piotr Trzonkowski, and Leopold Glasner. "Corneal Allografts: Factors for and against Acceptance." Journal of Immunology Research 2021 (October 3, 2021): 1–11. http://dx.doi.org/10.1155/2021/5372090.

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Cornea is one of the most commonly transplanted tissues worldwide. However, it is usually omitted in the field of transplantology. Transplantation of the cornea is performed to treat many ocular diseases. It restores eyesight significantly improving the quality of life. Advancements in banking of explanted corneas and progressive surgical techniques increased availability and outcomes of transplantation. Despite the vast growth in the field of transplantation laboratory testing, standards for corneal transplantation still do not include HLA typing or alloantibody detection. This standard pract

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Ostrovski, D. S., S. A. Borzenok, B. E. Malyugin, O. P. Antonova, M. Kh Khubetsova та T. Z. Kerimov. "Проблема получения клеточной культуры эндотелиальных клеток роговицы для регенеративных целей". Russian Journal of Transplantology and Artificial Organs 26, № 2 (2024): 135–44. http://dx.doi.org/10.15825/1995-1191-2024-2-135-144.

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Human posterior corneal epithelium (corneal endothelium) has limited proliferative activity both in vivo and in vitro. Disease or dysfunction in these cells leads to impaired corneal transparency of varying degrees of severity, up to blindness. Currently, the only effective standard treatment for corneal endothelial dysfunction is transplantation of donor cornea that contains a pool of healthy and functionally active cells. However, there is a global shortage of donor corneas, which has led to an unmet clinical need and the fact that only 1 patient out of 10 in need receives surgical treatment

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Cen, Yu-Jie, Wei Wang, and Yun Feng. "Preliminary studies of constructing a tissue-engineered lamellar corneal graft by culturing mesenchymal stem cells onto decellularized corneal matrix." International Journal of Ophthalmology 14, no. 1 (2021): 10–18. http://dx.doi.org/10.18240/ijo.2021.01.02.

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AIM: To construct a competent corneal lamellar substitute in order to alleviate the shortage of human corneal donor. METHODS: Rabbit mesenchymal stem cells (MSCs) were isolated from bone marrow and identified by flow cytometric, osteogenic and adipogenic induction. Xenogenic decellularized corneal matrix (XDCM) was generated from dog corneas. MSCs were seeded and cultured on XDCM to construct the tissue-engineered cornea. Post-transplantation biocompatibility of engineered corneal graft were tested by animal experiment. Rabbits were divided into two groups then underwent lamellar keratoplasty

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Guérin, Louis-Philippe, Gaëtan Le-Bel, Pascale Desjardins, et al. "The Human Tissue-Engineered Cornea (hTEC): Recent Progress." International Journal of Molecular Sciences 22, no. 3 (2021): 1291. http://dx.doi.org/10.3390/ijms22031291.

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Each day, about 2000 U.S. workers have a job-related eye injury requiring medical treatment. Corneal diseases are the fifth cause of blindness worldwide. Most of these diseases can be cured using one form or another of corneal transplantation, which is the most successful transplantation in humans. In 2012, it was estimated that 12.7 million people were waiting for a corneal transplantation worldwide. Unfortunately, only 1 in 70 patients received a corneal graft that same year. In order to provide alternatives to the shortage of graftable corneas, considerable progress has been achieved in the

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Brunette, Isabelle, Emilio I. Alarcon, and May Griffith. "Cornea Regeneration as an Alternative to Human Donor Transplantation." European Ophthalmic Review 09, no. 02 (2015): 111. http://dx.doi.org/10.17925/eor.2015.09.02.111.

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There is a need for an alternative to human donor corneas as the availability of good-quality tissues remains limited, with this situation potentially worsening as the population in many countries is progressively ageing. There have been numerous attempts to develop corneal equivalent as alternatives to donated human corneas as well as prostheses. In this short review, we focus on the efforts in bioengineering implants that promote regeneration by Canadian researchers, including our current team of authors. The examples of technologies developed that we describe include biomaterials that allow

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9

McTiernan, Christopher D., Fiona C. Simpson, Michel Haagdorens, et al. "LiQD Cornea: Pro-regeneration collagen mimetics as patches and alternatives to corneal transplantation." Science Advances 6, no. 25 (2020): eaba2187. http://dx.doi.org/10.1126/sciadv.aba2187.

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Transplantation with donor corneas is the mainstay for treating corneal blindness, but a severe worldwide shortage necessitates the development of other treatment options. Corneal perforation from infection or inflammation is sealed with cyanoacrylate glue. However, the resulting cytotoxicity requires transplantation. LiQD Cornea is an alternative to conventional corneal transplantation and sealants. It is a cell-free, liquid hydrogel matrix for corneal regeneration, comprising short collagen-like peptides conjugated with polyethylene glycol and mixed with fibrinogen to promote adhesion within

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10

Wan, Lu-Qin, Hui-Feng Wang, Chen Chen, et al. "Efficacy of rhNGF-loaded amniotic membrane transplantation for rabbit corneal epithelial and nerve regeneration." International Journal of Ophthalmology 14, no. 11 (2021): 1653–59. http://dx.doi.org/10.18240/ijo.2021.11.02.

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AIM: To evaluate the efficacy of recombinant human nerve growth factor-loaded amniotic membrane (rhNGF-AM) on corneal epithelial and nerve regeneration in rabbit model. METHODS: Freshly prepared human amniotic membrane (AM) were immersed into PBS buffer containing 100 or 500 μg/mL rhNGF for 15, 30, and 60min at 4℃. The in vitro release kinetics of rhNGF was measured with ELISA. For in vivo evaluation, the AM were immersed with 500 μg/mL rhNGF for 30min. Fifty-seven rabbits were selected to establish corneal epithelial defect model. In addition to the 19 rabbits in control group, 38 rabbits rec

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Qian, Ying, and M. Reza Dana. "Molecular mechanisms of immunity in corneal allotransplantation and xenotransplantation." Expert Reviews in Molecular Medicine 3, no. 18 (2001): 1–21. http://dx.doi.org/10.1017/s1462399401003246.

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Corneal allotransplantation is the most common and successful form of solid organ transplantation in humans. In uncomplicated cases, the two-year graft survival rate is over 90%. This extraordinary success can be attributed in part to various features of the normal cornea and anterior segment that together account for their ‘immune-privileged’ status. However, despite this success, a significant number of corneal grafts fail and immunological rejection remains by far the leading cause of graft failure. Studies on animal models of corneal transplantation have yielded a wealth of information on

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Staehlke, Susanne, Siddharth Mahajan, Daniel Thieme, Peter Trosan, and Thomas A. Fuchsluger. "Suppressing Pro-Apoptotic Proteins by siRNA in Corneal Endothelial Cells Protects against Cell Death." Biomedicines 12, no. 7 (2024): 1439. http://dx.doi.org/10.3390/biomedicines12071439.

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Corneal endothelial cells (CE) are critical for the cornea’s transparency. For severe corneal damage, corneal tissue transplantation is the most promising option for restoring vision. However, CE apoptotic cell death occurs during the storage of donor corneas for transplantation. This study used small interfering (si)RNA-mediated silencing of pro-apoptotic proteins as a novel strategy to protect CE against apoptosis. Therefore, the pro-apoptotic proteins Bax and Bak were silenced in the human corneal endothelial cell line (HCEC-12) by transfection with Accell™siRNA without any adverse effects

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13

Pedro do Valle Varela, João, Lara Gava, Shaira Salvadora Cunha Brito, et al. "CORNEAL TRANSPLANTATION." Health and Society 4, no. 02 (2024): 264–74. http://dx.doi.org/10.51249/hs.v4i02.2025.

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Corneal transplantation is an ophthalmic surgery that aims to restore vision in patients with corneal diseases. Advances in transplantation techniques and in the treatment of rejection have improved the results of this procedure, making it more effective and safer. This paper seeks to analyze the latest developments in transplantation techniques and treatments for rejection in corneal transplantation, highlighting not only scientific advances, but also the emotional and social aspects involved in this procedure, providing a comprehensive overview of the latest innovations in the field of corne

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Rodríguez-Barrientos, Carlos A., Amir Translateur-Grynspan, Judith Zavala, Jorge E. Valdez, Gisella Santaella, and Carmen Barraquer-Coll. "Intrastromal graft of anterior lens capsule. A substitute for Bowman layer graft transplantation for keratoconus." PLOS ONE 19, no. 11 (2024): e0306015. http://dx.doi.org/10.1371/journal.pone.0306015.

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Purpose The shortage of donor corneas limits Bowman layer transplantation for keratoconus. In this study, we evaluate the clinical outcome of porcine anterior lens capsule (xenograft) transplantation in the corneal stroma of a rabbit model as substitute for Bowman layer graft that is used in stromal transplantation for advanced keratoconus. Methods Transplantation of porcine anterior lens capsule in the corneal stroma was performed in four New Zealand white rabbits through the creation of a stromal pocket. Corneal transparency, central corneal thickness, and topographic characteristics of corn

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Patel, Dhaval, Radhika Tandon, Anita Ganger, Aarti Vij, Sanjeev Lalwani, and Adarsh Kumar. "Study of death to preservation time and its impact on utilisation of donor corneas." Tropical Doctor 47, no. 4 (2017): 365–70. http://dx.doi.org/10.1177/0049475517713406.

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To evaluate the impact of death-to-preservation time (DPT) on effective utilisation of donor corneas. In a prospective observational study conducted at our tertiary eye centre, donated corneas received over a 15-month period from November 2011 to January 2013 were evaluated. Donor age, donor refrigeration (done or not), DPT, endothelial cell density (ECD), corneal grading, clinical utilisation and surgical outcome after graft transplantation were noted. To analyse the impact of different DPT on donor cornea transplantation, primary outcome measures (corneal grading and endothelial cell density

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Liu, Yang, Chuanlei Zhang, Yanhui Kong, et al. "Preparation and Characterization of a Photo-Crosslinked Methacryloyl-Collagen Composite Film to Promote Corneal Nerve Regeneration via Surface Grafting of Taurine Molecules." International Journal of Molecular Sciences 24, no. 14 (2023): 11248. http://dx.doi.org/10.3390/ijms241411248.

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Blindness is frequently caused by corneal abnormalities, and corneal transplantation is the most effective treatment method. It is extremely important to develop high-quality artificial corneas because there are not enough donor corneas accessible for cornea transplantation. One of the most-often utilized materials is collagen, which is the primary component of natural cornea. Collagen-based corneal repair materials have good physicochemical properties and excellent biocompatibility, but how to promote the regeneration of the corneal nerve after keratoplasty is still a big challenge. In this r

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Shen, Lin, Peng Sun, Liqun Du, et al. "Long-Term Observation and Sequencing Analysis of SKPs-Derived Corneal Endothelial Cell-Like Cells for Treating Corneal Endothelial Dysfunction." Cell Transplantation 30 (January 1, 2021): 096368972110178. http://dx.doi.org/10.1177/09636897211017830.

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Corneal endothelial dysfunction is a principal cause of visual deficiency. Corneal transplantation is the most effective treatment for corneal endothelial dysfunction. However, a severe shortage of available donor corneas or human corneal endothelial cells (HCECs) remains a global challenge. Previously, we acquired corneal endothelial cell-like cells (CEC-like cells) derived from human skin-derived precursors (SKPs). CEC-like cells were injected into rabbit and monkey corneal endothelial dysfunction models and exerted excellent therapeutic effect. In this study, we prolonged the clinical obser

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Kandemir, Baran, Nesrin Tutaş Günaydın, Eren Göktaş, and Burak Tanyıldız. "Does Storage Time Affect the Outcomes of Split Corneal Transplantation to Reduce Corneal Donor Shortage? A Retrospective Study." INQUIRY: The Journal of Health Care Organization, Provision, and Financing 58 (January 2021): 004695802110458. http://dx.doi.org/10.1177/00469580211045846.

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Split cornea transplantation can reduce the shortage of donor corneas. Therefore, this study aimed to evaluate the effect of split graft storage time on the outcomes of split corneal transplantation through Descemet membrane endothelial keratoplasty (DMEK) and deep anterior lamellar keratoplasty (DALK) surgeries. Split corneal transplantation was performed in 80 eyes using 41 donor corneas. The mean before and after splitting storage times and total storage times were recorded. Donor corneal buttons and split grafts were stored in short-term solution at 4°C. In both surgeries (DMEK and DALK),

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Xie, Hua-Tao, Dan Zhao, Yang Liu, and Ming-Chang Zhang. "Umbilical Cord Patch Transplantation for Corneal Perforations and Descemetoceles." Journal of Ophthalmology 2017 (2017): 1–7. http://dx.doi.org/10.1155/2017/2767053.

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Purpose. To evaluate the clinical outcome of umbilical cord patch (UCP) transplantation for deep corneal ulcers with perforations and descemetoceles. Methods. In this retrospective, noncomparative, interventional case series, 11 eyes of 11 patients with corneal perforation or descemetocele were included. The thickness and microstructure of UCP were measured. All eyes were treated with UCP and amniotic membrane transplantation for corneal reconstruction. Corneal ulcer healing, corneal thickness, anterior chamber formation, and best-corrected visual acuity (BCVA) were recorded and analyzed. Resu

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Singh, Sujaya, Reena Kaur, Marium Jamaluddin, and Azida Juana. "ONE CORNEA TWO RECIPIENTS: FEEDING TWO BIRDS WITH ONE SCONE." Journal of Health and Translational Medicine 26, no. 1 (2023): 5–8. http://dx.doi.org/10.22452/jummec.vol26no1.2.

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The existing shortage of local donor corneas in our institution (University Malaya) and Malaysia, in general, prompted us to attempt the use of one donor cornea for two transplantation procedures; Descemet Stripping Endothelial Keratoplasty (DSEK) in a case of a pseudophakic bullous keratopathy (PBK) with underlying Fuchs endothelial dystrophy (FED) and lamellar patch graft in a case of limbal dermoid. The donor cornea was divided into anterior and posterior lamellae manually. The anterior corneal button was used as a patch graft for anterior lamellar keratoplasty in a 6-year-old patient with

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Hsiao, Ching-Hsi, Yih-Shiou Hwang, Wen-Yu Chuang, et al. "Prevalence and clinical consequences of cytomegalovirus DNA in the aqueous humour and corneal transplants." British Journal of Ophthalmology 103, no. 5 (2018): 666–71. http://dx.doi.org/10.1136/bjophthalmol-2018-312196.

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AimTo determine the prevalence and clinical consequences of cytomegalovirus (CMV) DNA in the aqueous and corneal tissues obtained at the time of corneal transplantation to evaluate the diagnostic value of PCR analysis in identifying patients at risk of postkeratoplasty CMV endotheliitis.MethodsThirty patients who underwent corneal transplantation were included in 2011. The aqueous, excised recipient corneas and donor corneoscleral rims were analysed by PCR for the presence of CMV DNA. The medical records of the patients were retrospectively reviewed and linked with PCR results.ResultsCMV DNA w

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Hashimoto, Yoshihide. "Research and development on therapeutic materials for corneal stromal disease consisting of transparent decellularised porcine corneas." Impact 2023, no. 3 (2023): 49–51. http://dx.doi.org/10.21820/23987073.2023.3.49.

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Investigations to discover potential corneal stromal substitutes to effectively treat corneal stromal disease tend to focus on transparent and bio-inert synthetic polymer materials and hydrogel materials. More recent studies are looking at alternative therapeutic materials that utilise corneas from pigs. Assistant Professor Yoshihide Hashimoto, Institute of Biomaterials and Bioengineering, Tokyo Medical and Dental University, Japan, is part of a team developing functional materials and therapeutic techniques to restore the function of damaged biological tissues and organs based on biomaterials

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Pereira Cruz, Giovanna Karinny, Marcos Antonio Ferreira Júnior, Oleci Pereira Frota, et al. "Cornea donation process and tissue quality for transplantation." PLOS ONE 16, no. 4 (2021): e0249927. http://dx.doi.org/10.1371/journal.pone.0249927.

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Introduction The quality of the corneal tissue can be influenced by several factors inherent to the recipient, donor, and to the donation and transplantation process. The donated corneal tissue can be classified by its quality as excellent, good, regular, bad, or unacceptable for transplantation, evaluating it in a slit lamp. Objective To analyze the relationship between the clinical and sociodemographic variables of the donors and the donation process and the classification of the quality of the corneal tissue collected for transplantation. Methods This is an epidemiologic study, retrospectiv

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Tan, Donald TH, Arundhati Anshu, and Jodhbir S. Mehta. "Paradigm Shifts in Corneal Transplantation." Annals of the Academy of Medicine, Singapore 38, no. 4 (2009): 332–39. http://dx.doi.org/10.47102/annals-acadmedsg.v38n4p332.

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Conventional corneal transplantation, in the form of penetrating keratoplasty (PK), involves full-thickness replacement of the cornea, and is a highly successful procedure. However, the cornea is anatomically a multi-layered structure. Pathology may only affect individual layers of the cornea, hence selective lamellar surgical replacement of only the diseased corneal layers whilst retaining unaffected layers represents a new paradigm shift in the field. Recent advancements in surgical techniques and instrumentation have resulted in several forms of manual, microkeratome and femto-second laser-

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Kobashigawa, K. K., B. C. Martins, M. C. B. Massoli, et al. "Microbiological profile of donor corneas stored for tectonic transplantation purposes in rabbits." Arquivo Brasileiro de Medicina Veterinária e Zootecnia 65, no. 1 (2013): 61–66. http://dx.doi.org/10.1590/s0102-09352013000100010.

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This study aimed to evaluate the microbiota of donor rabbit corneas stored for tectonic transplantation purposes. Swabs from both corneas of 20 rabbits were carefully collected and submitted to microorganism isolation and identification. After this first swab collection, rabbits were euthanized for reasons other than this project and the eyes were enucleated. The corneas were collected and stored to compose the cornea tissue bank. Corneas were stored in a 0.3% tobramycin solution at -20ºC. After 30 days, the corneas were thawed at room temperature and removed from the antibiotic. New swabs wer

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Jung, Il, and Byung Yi Ko. "Analysis in Results of Microbiologic Exam Related to Donor Corneas." Journal of the Korean Ophthalmological Society 63, no. 3 (2022): 236–41. http://dx.doi.org/10.3341/jkos.2022.63.3.236.

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Purpose: This study analyzed the microorganisms identified in donor corneas and their clinical significance.Methods: The medical records of 94 patients (114 eyes) who underwent keratoplasty and microbiological tests of the donor corneas from October 2008 to December 2020 at our hospital were reviewed retrospectively. During keratoplasty, we conducted microbiological tests of the corneoscleral rim of the donor cornea and preserving solution Optisol™-GS (Bausch & Lomb, Rochester, NY, USA), and examined the antibiotic susceptibility of bacterial isolates from the cultures.Results: Some isolat

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Silina, Elina, and Guna Laganovska. "Total Corneal Transplantation after Trauma." Acta Chirurgica Latviensis 17, no. 2 (2017): 37–38. http://dx.doi.org/10.1515/chilat-2017-0023.

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Abstract The first successful corneal transplantation is known since 1905, performed by Eduard Zirm (2). It has been implemented in order to restore vision in a variety of corneal diseases and after ocular traumas. The traditional technique for corneal transplantation, penetrating keratoplasty (PKP), refers to the full-thickness replacement of corneal tissue with a healthy donor graft (1). Authors report a well-documented case about successfully transplanted cornea after penetrating ocular trauma to improve visual outcome.

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ALBON, JULIE. "CORNEAL TRANSPLANTATION AND THE ARTIFICIAL CORNEA." Journal of Mechanics in Medicine and Biology 03, no. 01 (2003): 95–106. http://dx.doi.org/10.1142/s0219519403000636.

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The clinical need for an alternative to donor corneal tissue has encouraged much interest in recent years. An artificial cornea whether it be bio-engineered or a synthetic keratoprosthesis must fulfill the functions of the cornea it replaces: transparent, refractive surface, protection, non-immunogenic. A wide range of implants and biomedical devices have been developed in an attempt to correct corneal blindness. Limitation of existing biomaterials are evident when reviewing keratoprosthesis surgery complications. These include infection, intraocular inflammation, retromembrane formation, inad

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Ostadian, Farshad, Mahmoud Reza Panahi Bazzaz, Vahid Shahsavari, and Mohammad Sadegh Mirdehghan. "Evaluation of operations and causes of corneal transplantation in patients referred to the ophthalmology ward of Imam Khomeini Hospital in Ahvaz 2003 to 2019." Jundishapur journal of Medical Sciences 21, no. 6 (2023): 846–58. http://dx.doi.org/10.32598/jsmj.21.6.2787.

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Introduction: Corneal transplantation is one of the most common eye surgeries and the most common and successful tissue transplant in the world. The aim of this study was to determine the frequency of causes and types of corneal transplantation in patients referred to the ophthalmology ward of Imam Khomeini Hospital in Ahvaz from 2003 to 2018. Methods: This descriptive study was performed on the records of the patients who underwent corneal transplantation. The information included age, sex, cause of transplantation, sex and age of the donor, need for re-transplantation, reason for re-transpla

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Dydyk, Martyna, Ewa Sikorska, Piotr Sikorski, Robert Tomaszewski, and Dorota Kopacz. "The use of stem cells and keratoprosthesis – special surgery types for corneal disorders (a narrative review)." Journal of Medical Science 93, no. 3 (2024): e1045. https://doi.org/10.20883/medical.e1045.

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Corneal disease and damage can arise from various causes, including infections, corneal dystrophies, trauma and improper contact lens use. These factors can lead to corneal blindness which greatly decreases the patient’s quality of life. Although corneal transplantation is a viable treatment option for many patients, the risk of rejection after corneal transplantation remains a concern. Severe corneal damage requires special methods of treatment, which are still sought after. This narrative review aims to summarize the use of stem cells and keratoprosthesis in the treatment of corneal diseases

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Ahmedov, A. K., T. Z. Kerimov, Kh D. Tonaeva, B. E. Malygin, and S. A. Borzenok. "Technology for obtaining an ultrathin posterior lamellar corneal graft at the Eye Tissue Bank." Russian Journal of Transplantology and Artificial Organs 22, no. 3 (2020): 167–73. http://dx.doi.org/10.15825/1995-1191-2020-3-167-173.

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Objective: to develop technologies for preoperative preparation of the posterior lamellar corneal graft based on our own formulation of the preservation medium for optimal dehydration of the donor cornea and a technique for cutting out an ultrathin flap using an optimized method at the Eye Tissue Bank. Materials methods. In a series of experimental studies, we obtained data on the hydration level of cadaveric donor corneas that were preserved in various solutions at different observation periods. Using 16 corneas, analytical weighing and pachymetry were performed via optical coherence tomograp

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Orash Mahmoud Salehi, Amin, Saeed Heidari-Keshel, Seyed Ali Poursamar, et al. "Bioprinted Membranes for Corneal Tissue Engineering: A Review." Pharmaceutics 14, no. 12 (2022): 2797. http://dx.doi.org/10.3390/pharmaceutics14122797.

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Corneal transplantation is considered a convenient strategy for various types of corneal disease needs. Even though it has been applied as a suitable solution for most corneal disorders, patients still face several issues due to a lack of healthy donor corneas, and rejection is another unknown risk of corneal transplant tissue. Corneal tissue engineering (CTE) has gained significant consideration as an efficient approach to developing tissue-engineered scaffolds for corneal healing and regeneration. Several approaches are tested to develop a substrate with equal transmittance and mechanical pr

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Malyugin, B. E., S. A. Borzenok, O. P. Antonova, D. S. Ostrovskii, and Z. R. Ebzeeva. "Transplantation of endothelial cell suspension in an ex vivoexperiment." Fyodorov journal of ophthalmic surgery, no. 4 (December 28, 2023): 86–92. http://dx.doi.org/10.25276/0235-4160-2023-4-86-92.

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Relevance. Corneal transplantation with entire cornea or anormal layers replacement remains the only treatment option for patients with corneal endothelium pathology. Selective endothelial replacement was introduced into practice two decades ago, namely posterior lamellar keratoplasty, which has advantages over penetrating keratoplasty. Improvements of this method led to technique creation where isolated Descemet's membrane with endothelium monolayer without stromal layer is transplanted. This technology is associated with risks of intra- and postoperative complications, and is difficult to pe

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Oganesyan, Oganes G., S. S. Yakovleva, M. P. Kharlampidi, and A. A. Grdikanyan. "The rationale application of donor material: original ten years experience, possible ways of development and literature data." Medical Journal of the Russian Federation 22, no. 4 (2016): 193–97. http://dx.doi.org/10.18821/0869-2106-2016-22-4-193-197.

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Lately, layer-wise keratoplasty became the first operation of choice in case of pathology of cornea. In view of satisfying results of endothelial surgery similar operations are implemented at earlier stages that increases need in donor tissue. With increasing of life span of population, also increases number of patients in need of transplantation of cornea. The number of intact cadaver cornea decreases because of stable increase of surgical interventions on front segment of eye. The present article presents original ten years experience concerning optimization of application of donor tissue an

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Bachmann, Björn. "Ripasudil zur antiangiogenen Therapie bei Keratoplastik." Kompass Ophthalmologie 7, no. 3 (2021): 128–29. http://dx.doi.org/10.1159/000518817.

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Corneal allograft survival is mediated by the variety of immunological reactions and wound healing process. Our aim was to explore the effects of topical administration of ripasudil, a selective Rho-associated coiled-coil protein kinase inhibitor, on corneal allograft survival. Ripasudil was administered to mice thrice a day after allogeneic corneal transplantation. Corneal graft survival, opacity, neovascularization, re-epithelization, immune cell infiltration, and mRNA levels of angiogenic and pro-inflammatory factors in the grafted cornea and draining lymph nodes (dLNs) were evaluated with

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Chen, Yingxin, Congling Liao, Minghong Gao, et al. "Efficacy and Safety of Corneal Transplantation Using Corneas from Foreign Donors versus Domestic Donors: A Prospective, Randomized, Controlled Trial." Journal of Ophthalmology 2015 (2015): 1–10. http://dx.doi.org/10.1155/2015/178289.

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Purpose. To assess the efficacy and safety of corneal transplantation using corneas from foreign donors.Methods. One hundred and eight patients needing therapeutic penetrating keratoplasty were randomly divided into 2 groups (54 cases/group): foreign group using foreign donor corneas and domestic group using domestic donor corneas. Clinical outcome and incidence of postoperative complications were compared between groups.Results. No significant difference with respect to the therapeutic outcome and postoperative Best Corrected Visual Acuity (BCVA) and neovascularization by final follow-up was

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Fuest, Matthias, Gary Hin-Fai Yam, Jodhbir S. Mehta, and Daniela F. Duarte Campos. "Prospects and Challenges of Translational Corneal Bioprinting." Bioengineering 7, no. 3 (2020): 71. http://dx.doi.org/10.3390/bioengineering7030071.

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Corneal transplantation remains the ultimate treatment option for advanced stromal and endothelial disorders. Corneal tissue engineering has gained increasing interest in recent years, as it can bypass many complications of conventional corneal transplantation. The human cornea is an ideal organ for tissue engineering, as it is avascular and immune-privileged. Mimicking the complex mechanical properties, the surface curvature, and stromal cytoarchitecure of the in vivo corneal tissue remains a great challenge for tissue engineering approaches. For this reason, automated biofabrication strategi

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Choi, Alexander J., Brenna S. Hefley, Hannah A. Strobel, et al. "Fabrication of a 3D Corneal Model Using Collagen Bioink and Human Corneal Stromal Cells." Journal of Functional Biomaterials 16, no. 4 (2025): 118. https://doi.org/10.3390/jfb16040118.

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Corneal transplantation remains a critical treatment option for individuals with corneal disorders, but it faces challenges such as rejection, high associated medical costs, and donor scarcity. A promising alternative for corneal replacement involves fabricating artificial cornea from a patient’s own cells. Our study aimed to leverage bioprinting to develop a corneal model using human corneal stromal cells embedded in a collagen-based bioink. We generated both cellular and acellular collagen I (COL I) constructs. Cellular constructs were cultured for up to 4 weeks, and gene expression analysis

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Hu, Shi-Si, Xu-Yun Meng, Bo-Wen Ouyang, Zhen-Duo Yang, Xiao-Dan Chen, and Xing-Wu Zhong. "Treatment of superficial corneal opacities with corneal stromal lenticule obtained through SMILE surgery." International Journal of Ophthalmology 17, no. 12 (2024): 2221–28. http://dx.doi.org/10.18240/ijo.2024.12.09.

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AIM: To evaluate the clinical efficacy and feasibility of superficial corneal opacities treated by excimer laser phototherapeutic keratectomy (PTK) combined with small incision lenticule extraction (SMILE)-derived corneal stromal lenticule transplantation. METHODS: A retrospective interventional case series of nine patients aged 12–59y with superficial corneal opacity caused by different pathologies who underwent standardized PTK combined with SMILE-derived corneal stromal lenticule transplantation was examined. Lenticule patches were fixed with fibrin glue. All patients underwent pre- and pos

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Clahsen, Thomas, Christian Büttner, Niloofar Hatami, André Reis, and Claus Cursiefen. "Role of Endogenous Regulators of Hem- And Lymphangiogenesis in Corneal Transplantation." Journal of Clinical Medicine 9, no. 2 (2020): 479. http://dx.doi.org/10.3390/jcm9020479.

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Under normal conditions, the cornea, being the transparent “windscreen” of the eye, is free of both blood and lymphatic vessels. However, various diseases of the eye, like infections, can interfere with the balance between promoting and inhibiting factors, which leads to ingrowth of blood and lymphatic vessels. The newly formed lymphatic vessels increase the risk of graft rejection after subsequent corneal transplantation. Corneal transplantation is one of the most commonly performed transplantations worldwide, with more than 40,000 surgeries per year in Europe. To date, various anti-hem- and

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De Leon, Patricia Mae, and Heherson Cabrera. "Analyzing Porcine Corneal Xenograft Compatibility: In Silico Insights on Graft Outcomes." Transplantology 5, no. 3 (2024): 193–207. http://dx.doi.org/10.3390/transplantology5030019.

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Background: Corneal transplantation faces significant challenges due to the shortage in donor corneas. Porcine corneas have emerged as a potential solution due to their similarities in biomechanical properties with pigs, yet xenoimmune rejection poses an obstacle to their efficacy. Methods: In this study, in silico methods were employed to analyze the compatibility of porcine corneal xenografts, focusing on two key aspects: the comparison of corneal matrix proteins and investigation of the immunological mediators and pathways involved in corneal graft rejection. The amino acid sequences of the

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Parker, Jack, Philip Dockery, Ana Preda-Naumescu, et al. "Descemet Membrane Endothelial Keratoplasty and Bowman Layer Transplantation: An Anatomic Review and Historical Survey." Ophthalmic Research 64, no. 4 (2021): 532–53. http://dx.doi.org/10.1159/000516093.

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For nearly a century, the definitive treatment of many corneal dystrophies and ectactic disorders was limited to penetrating keratoplasty, but over the past 2 decades, a surge of surgical innovation has propelled the treatment of many corneal diseases to more targeted approaches with significantly better visual outcomes. Anterior stromal diseases were first changed through endothelial-sparing techniques, such as deep anterior lamellar keratoplasty, but have more recently transitioned to stromal-sparing approaches. Ultraviolet corneal crosslinking strengthens the cornea and halts progression of

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Ostrovsky, D. S., S. A. Borzenok, B. E. Malyugin, O. P. Antonova, M. Kh Khubetsova, and T. Z. Kerimov. "Preparation and evaluation of a suspension of human corneal endothelial cells isolated from the eyes of cadaveric donors for transplantation in an <em>ex vivo</em> experiment." Russian Journal of Transplantology and Artificial Organs 26, no. 1 (2023): 103–12. http://dx.doi.org/10.15825/1995-1191-2024-1-103-112.

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Background. According to the World Health Organization, corneal diseases are one of the major causes of blindness globally. Endothelial dystrophy is one of the etiological factors leading to corneal diseases. The corneal endothelium is a monolayer of cells with virtually no mitotic activity. When the density of corneal endothelial cells falls below a critical threshold, the endothelium loses its ability to regulate corneal stromal hydration. This leads to corneal clouding and, consequently, to reduced visual acuity and quality of life of the patient. In this regard, various keratoplasty method

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Nariya, Yuta, Takashi Ono, Yuichi Asahina, et al. "A Case of Transparent Cornea Maintained after Removal of Dislocated Graft from Descemet’s Stripping Automated Endothelial Keratoplasty." Case Reports in Ophthalmology 15, no. 1 (2024): 518–24. http://dx.doi.org/10.1159/000539392.

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Introduction: Corneal graft detachment is a major postoperative complication of Descemet’s stripping automated endothelial keratoplasty (DSAEK). When a corneal graft becomes detached, corneal endothelial function generally fails, and repeat corneal transplantation is required. Herein, we report a rare case in which a transparent cornea was maintained after the removal of a dislocated DSAEK graft. Case Presentation: A 79-year-old woman with a residual lens cortex who had undergone cataract surgery was referred to our hospital. The cortex was removed, and bullous keratopathy progressed. Six mont

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Kusano, Mao, Yasser Helmy Mohamed, Masafumi Uematsu, et al. "Whole Corneal Descemetocele." Medicina 59, no. 10 (2023): 1780. http://dx.doi.org/10.3390/medicina59101780.

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Background and Objectives: To report a case of microbial keratitis complicated by severe corneal melting and whole corneal descemetocele. Methods: A 72-year-old male farmer presented with a right corneal ulcer involving nearly the entire cornea, which was almost completely melted down with the remaining Descemet’s membrane (DM). The pupil area was filled with melted necrotic material, with the intraocular lens partially protruding from the pupil and indenting the DM. Corneal optical coherence tomography (OCT) examination revealed a corneal thickness of 37 µm that was attached to its back surfa

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Pereira Cruz, Giovanna Karinny, Marcos Antonio Ferreira Júnior, Viviane Euzébia Pereira Santos, et al. "The donation-transplantation process and corneal graft failure: A case-control study." PLOS One 20, no. 5 (2025): e0321225. https://doi.org/10.1371/journal.pone.0321225.

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Introduction The survival after a corneal transplantation depends on several factors, many of which are not fully known. Objective The present study aimed to verify the relationship between the factors inherent to the donation-transplantation process in graft failure. Materials and methods Longitudinal case-control study carried out in a reference service in corneal transplantation in northeastern Brazil, which included transplanted patients with corneal graft failure and their respective controls. The sample consisted of 27 cases of graft failure and 54 controls. Results A total of 427 transp

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Tarsitano, Martine, Maria Chiara Cristiano, Massimo Fresta, Donatella Paolino, and Concetta Rafaniello. "Alginate-Based Composites for Corneal Regeneration: The Optimization of a Biomaterial to Overcome Its Limits." Gels 8, no. 7 (2022): 431. http://dx.doi.org/10.3390/gels8070431.

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For many years, corneal transplantation has been the first-choice treatment for irreversible damage affecting the anterior part of the eye. However, the low number of cornea donors and cases of graft rejection highlighted the need to replace donor corneas with new biomaterials. Tissue engineering plays a fundamental role in achieving this goal through challenging research into a construct that must reflect all the properties of the cornea that are essential to ensure correct vision. In this review, the anatomy and physiology of the cornea are described to point out the main roles of the cornea

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Pant, Om Prakash, Ji-long Hao, Dan-dan Zhou, and Cheng-wei Lu. "Tectonic keratoplasty using femtosecond laser lenticule in pediatric patients with corneal perforation secondary to blepharokeratoconjunctivitis: a case report and literature review." Journal of International Medical Research 47, no. 5 (2019): 2312–20. http://dx.doi.org/10.1177/0300060519841163.

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Blepharokeratoconjunctivitis secondary to ocular demodicosis in the pediatric population is often neglected and may result in a serious sight-threatening condition. In severe cases, it can lead to corneal perforation necessitating urgent corneal transplantation. However, the shortage and high cost of donor corneas is the foremost limitation of keratoplasty in developing countries. Small-incision lenticule extraction is an advanced flapless femtosecond laser refractive procedure in which an intrastromal corneal lenticule is detached and removed to correct myopia and myopic astigmatism. We herei

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Musa, Mutali, Marco Zeppieri, Ehimare S. Enaholo, Ekele Chukwuyem, and Carlo Salati. "An Overview of Corneal Transplantation in the Past Decade." Clinics and Practice 13, no. 1 (2023): 264–79. http://dx.doi.org/10.3390/clinpract13010024.

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The cornea is a transparent avascular structure located in the front of the eye that refracts light entering the eyes and also serves as a barrier between the outside world and the internal contents of the eye. Like every other body part, the cornea may suffer insult from trauma, infection, and inflammation. In the case of trauma, a prior infection that left a scar, or conditions such as keratoconus that warrant the removal of all or part of the cornea (keratoplasty), it is important to use healthy donor corneal tissues and cells that can replace the damaged cornea. The types of cornea transpl

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Weladi, Ujwala, and Snehal Bonde Chaurasia. "Potential of hospital corneal retrieval program in tertiary care center." Journal of Clinical Ophthalmology and Research 13, no. 1 (2025): 82–85. https://doi.org/10.4103/jcor.jcor_94_24.

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Context: In developing nations, corneal diseases are the second leading cause of blindness. Despite the effectiveness of corneal transplantation, India struggles with a substantial corneal blind population and inadequate access to quality donor corneal tissue. Addressing this gap between demand and supply is essential to combat the prevalence of corneal blindness. Aims: The aim of this study is to study the potential of hospital-based cornea retrieval program (HRCP) at tertiary care center. Subjects and Methods: A retrospective, record-based study was conducted, encompassing all hospital death

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