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Artykuły w czasopismach na temat „Double disc synergy test”

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Data publikacji: 3 czerwca 2025

Data aktualizacji: 31 lipca 2025

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1

Biswas, SM, MRA Mia, N. Ara, M. Ibrahim, TA Nasir, and S. Yunus. "Comparison of Three Dimensional Test and Double Disc Synergy Test for detection of Extended Spectrum ?-Lactamase (ESBL) producing Gram negative bacteria." Pulse 6, no. 1-2 (2014): 12–19. http://dx.doi.org/10.3329/pulse.v6i1-2.20328.

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Extended spectrum ?-lactamase (ESBLs), produced by Gram negative organisms, are enzymes capable of hydrolyzing extended spectrum cephalosporins, penicillins and monobactam but inactive against cephamycin and imipenem. Detection of some ESBL strains are missed by Double disc synergy test but detected by three dimensional test. So this study was carried out to see the rate of ESBL producer by three dimensional test and double disc synergy test among Gram negative bacteria. Total 110 Gram negative isolates were studied, among them 30 were wound swab isolates and 80 were laboratory isolates, of wh

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2

Karim Chowdhury, Abu Hena Md Saiful, Sukumar Nandi, Mahbubur Rahman, et al. "Comparison Between Phenotypic Confirmatory Test & Double Disc Synergy Test in Detection of Extended Spectrum b-Lactamases Producers Among Gram-Negative Bacilli." Chattagram Maa-O-Shishu Hospital Medical College Journal 15, no. 2 (2017): 3–8. http://dx.doi.org/10.3329/cmoshmcj.v15i2.31795.

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Background: Extended-Spectrum b-Lactamases (ESBLs) producing bacteria are increasing in number and causing more severe infections because of their continuous mutation and multidrug resistance property which make its treatment difficult. Thus reliable, sensitive and low cost method to detect ESBLs producers, therefore, is of major interest. The present study was undertaken to compare the sensitivity between double disc synergy test & phenotypic confirmatory test to detect ESBLs producing bacteria.Methods: All the isolates were identified by standard procedure of identification & isolate

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Malik, Taqdees, and Asma Naim. "Occurrence of ESBLs in Clinical Isolates of Klebsiella Species and Comparative Analysis of Phenotypic Detection Methods." Anti-Infective Agents 18, no. 3 (2020): 255–60. http://dx.doi.org/10.2174/2211352517666191114130036.

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Aims: Drug resistance enzymes like beta-lactamases and Extended-Spectrum Beta- Lactamases (ESBLs) are mostly produced in the response of mutation and a few other factors. These enzymes are plasmid-mediated that can confer resistance against several β-lactam agents including cephalosporin and extended-spectrum cephalosporin. This study is conducted to determine the frequency of ESBL producing Klebsiella strains and to compare the phenotypic detection methods among various clinical samples collected from a commercial centralized Microbiology diagnostic laboratory. Materials and Methods: 125 Kleb

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Diab, Atef M., M. H. Abul-Aziz, Iman M. A. El-Kholy та Mona A. Rezk. "MODIFIED DOUBLE-DISC SYNERGY TEST (MDDST) VERSUS DOUBLE-DISC SYNERGY TEST (DDST) FOR DETECTION OF ESBL(S) IN AMPC Β-LACTAMASE-PRODUCING KLEBSIELLA CLINICAL ISOLATES". European Chemical Bulletin 7, № 2 (2018): 89. http://dx.doi.org/10.17628/ecb.2018.7.89-92.

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Hassani, Umesh Santlal, та Rashmi Mahalle. "Phenotypic methods for detection of metallo-β-lactamase (MBL) production in multidrug resistant gram negative bacilli – comparative study". Journal of Applied Pharmaceutical Research 11, № 3 (2023): 29–35. http://dx.doi.org/10.18231/j.joapr.2023.11.3.29.35.

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Introduction: One of the most common mechanism of resistance of bacterial pathogens to β-lactam antibiotics is production of β-lactamase, there are different types of Beta lactamases, which are expressed by drug resistant gram-negative bacteria. Carbapenemases (Metallo beta lactamases/MBL) are the β-lactamases with the widest spectrum of activity. Early detection of MBL-producing organisms is crucial to establish appropriate antimicrobial therapy and to prevent their interhospital and intrahospital dissemination. Several phenotypic methods are available for the detection of MBL producing bacte

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6

Amin, Aalia. "Antibiotic Sensitivity Pattern and ESBL Detection among Various Clinical Isolates of Escherichia coli." International Journal of Current Microbiology and Applied Sciences 13, no. 3 (2024): 27–33. http://dx.doi.org/10.20546/ijcmas.2024.1303.002.

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The ESBL producing isolate of E.coli among other clinical isolates has been increasing over the past few years resulting in limitation of therapeutic options. Bacteria produces ESBL poses a major problem for clinical therapautics. A cross sectional study was carried out in the Department of Microbiology Sharda Hospital School of Medical Science and Research, Sharda University, Greater Noida. 204 E.coli were isolated from various clinical samples which consists of urine, pus, sputum, blood, stool, swab, and different body fluids from both IPD & OPD patents were included in this study. Sampl

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Mangla, Aarushi, Hetal Vasani, and M. M. Vegad. "Evaluation of different phenotypic techniques in detection of metallo-betalactamases in pseudomonas aeruginosa." IP International Journal of Medical Microbiology and Tropical Diseases 8, no. 3 (2022): 242–46. http://dx.doi.org/10.18231/j.ijmmtd.2022.047.

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Metallo-beta-lactamase (MBL) producing Pseudomonas is a worrisome pathogen to hospital infection control due to production of resistance to multiple antibiotics. So, treatment options are narrowed down to only few antibiotics which will result in high morbidity and mortality. This study was conducted with the aim to detect prevalence of MBL producing Pseudomonas aeruginosa and to compare the different phenotypic methods for the detection of MBL production in Imipenem resistant clinical isolates of Pseudomonas aeruginosa. : In this prospective study total of 14,145 different clinical samples re

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Vamsi, K. Sreeja, S. Rama Moorthy, T. S. Murali, et al. "Phenotypic Methods for the Detection of Metallo-Beta-Lactamase Production by Gram-negative Bacterial Isolates from Hospitalized Patients in A Tertiary Care Hospital in India." Journal of Pure and Applied Microbiology 15, no. 4 (2021): 2019–26. http://dx.doi.org/10.22207/jpam.15.4.23.

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Drug-resistant bacteria are a global health concern owing to the high morbidity and mortality they can cause, especially in countries such as India. Gram-negative bacteria, including Enterobacteriaceae, Pseudomonas, and Acinetobacter, are primarily responsible for expanding the scope of drug resistance. These antibiotic-resistant pathogens are particularly associated with serious infections in hospitals. The production of carbapenemase by gram-negative bacteria appears to be the major reason for their resistance to carbapenems. The study was a prospective study done from March 2018 to December

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Hamdani, Muhammad Jen, Natsir Djide, and Mansyur Arif. "Incidence of Klebsiella pneumoniae producing Metallo Beta-Lactamase (MBL) at RSUP Dr. Wahidin Sudirohusodo Makassar." Sasambo Journal of Pharmacy 3, no. 1 (2022): 6–10. http://dx.doi.org/10.29303/sjp.v3i1.111.

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Bacterial resistance to antibiotic is one of the factors triggering infection therapy failure. This study was conducted to determine the prevalence of carbapenem-resistance Klebsiella pneumoniae infection and the phenotype of carbapenem-resistant Metallo-Beta-Lactamase (MBL)-producing Klebsiella pneumoniae isolates at RSUP Dr. Wahidin Sudirohusodo Makassar. This study included Klebsiella pneumoniae identification on each infectious patient’s isolates. The sensitivity test of antibiotics, phenotype confirmatory test, and MBL phenotypic test were conducted using agar diffusion Kirby-Bauer, Vitek

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Mahdi, Hozan Salahaddin, and Sherko A. Omer. "DOUBLE DISK SYNERGY TEST AND BIOFILM FORMATION IN CLINICAL ISOLATES OF PSEUDOMONAS AERUGINOSA." Journal of Sulaimani Medical College 9, no. 3 (2019): 201–8. http://dx.doi.org/10.17656/jsmc.10207.

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Gautam, S., N. R. Bhattarai, K. Rai, A. Poudyal, and B. Khanal. "Detection of bla NDM-1 Encoding Imepenemase among the Imipenem-Resistant Gram-Negative Bacilli Isolated from Various Clinical Samples at a Tertiary Care Hospital of Eastern Nepal: A Descriptive Cross-Sectional Study." International Journal of Microbiology 2020 (July 27, 2020): 1–5. http://dx.doi.org/10.1155/2020/8861204.

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Background. Carbapenem resistance among Gram-negative isolates caused by the production of the metallo-β-lactamase (MBL) enzyme is being increasingly reported worldwide. One of the newly emerged metallo-β-lactamases is New Delhi metallo-β-lactamase. Data regarding its occurrence in hospital setting and percentage prevalence among different Gram-negative bacterial isolates are lacking in our part. This study has been undertaken for determining the presence of the bla NDM-1 gene among the clinical isolates of imipenem-resistant Gram-negative bacteria in a tertiary care center in Dharan, Nepal. M

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Chowdhury, Rifat Asma, Sabrina Sharmin, Abu Hena Md Saiful Karim Chowdhury, Nasima Akter, Shakeel Ahmed, and Gulshan Nigar Choudhury. "Comparison of combined disc synergy test and double disc synergy test for phenotypic detection of metallo-ß-lactamase among the clinical isolates of gram-negative bacilli." International Journal of Research in Medical Sciences 10, no. 7 (2022): 1402. http://dx.doi.org/10.18203/2320-6012.ijrms20221782.

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Background: Metallo-β-lactamases (MBL) have a wide spectrum of activity and they confer a higher level of resistance to all β-lactams antibiotics including Carbapenem. The active site in MBLs contains either 1 or 2 Zn2+ ions for their catalytic mechanism. All MBLs share a common feature of being inhibited by EDTA. Metallo-β-lactamase-producing gram-negative bacteria are the most important nosocomial pathogens. The present study was conducted to detect Metallo-β-lactamase (MBL) production in gram-negative bacilli by Combined Disc Synergy Test (CDST) and Double Disc Synergy Test (DDST) with 0.1M

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Saha, Mili Rani, Sanya Tahmina Jhora, Shikha Paul, and Durdana Chowdhury. "Isolation of ESBL producing organisms by Double Disc Synergy Test in tertiary care hospital." Bangladesh Journal of Medical Microbiology 7, no. 2 (2013): 21–24. http://dx.doi.org/10.3329/bjmm.v7i2.19330.

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Extended spectrum beta-lactamase (ESBL) producing organisms pose unique challenges to clinical microbiologists, clinicians, infection control professionals and antibacterial discovery scientists. The study was done to determine ESBL producing strains by a most feasible and accurate phenotypic method. Urine, surgical wound swab, burn wound swab and pus samples were taken from Sir Salimullah Medical College and Mitford Hospital and from Burn Unit of Dhaka Medical College Hospital from January 2011 to December 2011. ESBL producers were detected by Double Disc Synergy Test. From 354 different samp

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14

Anwar, Khanda Abdulateef. "Detection of O25B-ST131 clone and bla_CTX-M-15 gene in Escherichia coli isolated from patients with COVID-19." PeerJ 13 (March 6, 2025): e19011. https://doi.org/10.7717/peerj.19011.

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Background Isolation of blaCTX-M family of extended-spectrum beta-lactamases (ESBL) is a challenge in the field of microbiology in our locality that makes treatment fail and disseminate quickly. Objectives To determine the prevalence of blaCTX-M-15 ESBL gene in Escherichia coli clone O25B-ST131 isolated from COVID-19 patients with different infections. Methods This cross-sectional study was conducted on 528 patients hospitalized due to COVID-19 infection with various symptoms from April to September 2021. Using standard culturing techniques, E. coli were isolated from patients’ various samples

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15

Khan, Salman, Arghya Das, Deepali Vashisth, et al. "Evaluation of a simple method for testing aztreonam and ceftazidime-avibactam synergy in New Delhi metallo-beta-lactamase producing Enterobacterales." PLOS ONE 19, no. 5 (2024): e0303753. http://dx.doi.org/10.1371/journal.pone.0303753.

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NDM-producing carbapenem-resistant bacterial infections became a challenge for clinicians. Combination therapy of aztreonam and ceftazidime-avibactam is a prudent choice for these infections. However, there is still no recommendation of a practically feasible method for testing aztreonam and ceftazidime-avibactam synergy. We proposed a simple method for testing aztreonam and ceftazidime-avibactam synergy and compared it with reference broth micro-dilution and other methods. Carbapenem-resistant Enterobacterales clinical isolates were screened for the presence of the NDM gene by the Carba R tes

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Chitrabanu, N. Ashwin, and Shrikara Mallya. "Identification, Speciation and Antibiogram along with Detection of Metallo Beta-lactamase Production in Acinetobacter Isolated from Clinical Samples in a Tertiary Care Hospital." Journal of Pure and Applied Microbiology 15, no. 2 (2021): 839–44. http://dx.doi.org/10.22207/jpam.15.2.38.

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Acinetobacter species are gram negative non fermenters, which are important nosocomial pathogens involved in various outbreaks in hospitals due to widespread resistance to majority antibiotics. The aim of this study is to speciate Acinetobacter isolated from clinical samples, to assess the antibiotic sensitivity pattern and to detect the production of metallo-β-lactamase by double disc synergy test. The study was conducted in the department of microbiology, A. J Institute of Medical Sciences. All clinical samples were subjected to gram stain & cultured; the Acinetobacter isolates obtained

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17

Garba, L., M. Yushaâ€™u, M. M. Abdullahi, et al. "Effectiveness of Double Discs Synergy Test in the Confirmation of Extended Spectrum Î²-lactamase (ESÎ²L) Production." Journal of Biochemistry, Microbiology and Biotechnology 6, no. 2 (2018): 15–18. http://dx.doi.org/10.54987/jobimb.v6i2.428.

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Extended spectrum beta lactamases (ESÎ²Ls) are enzymes which have evolved from bacteria upon exposure to penicillins antibiotics. Such bacteria have developed resistance to Beta lactam antibiotics and their relatives such as cephalosporins (extended spectrum beta lactam antibiotics). The enzymes represent the major source of multidrug resistance in gram negative bacteria. Different methods of confirming ESÎ²L production from bacteria have been described. Most of these methods suggest screening the test isolates in accordance with decrease in susceptibility to extended-spectrum cephalosporins i

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G, Kaviyarasan, Rajamanikandan Kcp, Sabarimuthu M, Ramya S та Arvind Prasanth D. "ANTIMICROBIAL SUSCEPTIBILITY AND DETECTION METHODS FOR THE EXTENDED‑SPECTRUM β-LACTAMASES PRODUCING ENTEROBACTERIACEAE FROM CLINICAL SAMPLES". Asian Journal of Pharmaceutical and Clinical Research 11, № 5 (2018): 139. http://dx.doi.org/10.22159/ajpcr.2018.v11i5.24356.

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Objectives: Detection of extended-spectrum β-lactamases (ESBLs) is crucial for the infection control and antibiotic choice in healthcare settings. The aim of this study is to develop a standardized, inexpensive, and simple approach that is able to detect ESBL-producing Enterobacteriaceae isolates.Methods: Isolates those were resistant to at least one of the three indicator cephalosporins (cefotaxime, cefpodoxime, and ceftazidime) were tested for ESBL production using the double disc synergy test (DDST), combined disc synergy test (CDST) test and genotypic detection of the responsible gene for

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Saha, Mili Rani, Sanya Tahmina Jhora, Tarek Mahbub Khan, Shikha Paul, and Durdana Chowdhury. "Detection of SHV Gene from Extended-Spectrum Beta-Lactamases (ESBLs) Producing Isolates in a Tertiary Care Hospital." Bangladesh Journal of Medical Microbiology 8, no. 2 (2017): 14–18. http://dx.doi.org/10.3329/bjmm.v8i2.31093.

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Most ESBL Extended-Spectrum beta-lactamases (ESBLs) producing isolates carry TEM and SHV type betalactamases. Among these two types beta-lactamases SHV type was reported nosocomial infection outbreak in many countries in the world. The goal of this study was to see the rate of ESBLs production and also to detect SHV gene in those isolates recovered from a tertiary care hospital. A total of 354 samples were collected from Sir Salimullah Medical College and Mitford Hospital and from Burn Unit of Dhaka Medical College Hospital from January 2011 to December 2011. To detect ESBLs, Gram-negative org

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Mihajlovic-Ukropina, Mira, Anika Trudic, Zora Jelesic, Deana Medic, Biljana Milosavljevic, and Branka Zivlak. "Detection of carbapenemase genes in Klebsiella pneumoniae isolates." Srpski arhiv za celokupno lekarstvo 144, no. 5-6 (2016): 307–11. http://dx.doi.org/10.2298/sarh1606307m.

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Introduction. Klebsiella pneumoniae is one of the leading causes of serious hospital-acquired infections worldwide among Enterobacteriaceae species. It is the most common producer of carbapenemases in many parts of the world. Objective. The aim of the study was to determine which enzymes were responsible for resistance to carbapenems in Klebsiella pneumoniae strains isolated at the Centre of Microbiology of Public Health Institute of Vojvodina. Methods. A total of 29 Klebsiella pneumoniae non-duplicated strains resistant to at least one carbapenem isolated from clinical samples of hospitalized

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Nazia, Anwar, Yasmin Tarannum та Alam Farmood. "Detection of Metallo-Βeta-Lactamases Producing Pseudomonas aeruginosa which are Received from the Patients in our Laboratory at Dmch, Laheriasarai, Bihar". International Journal of Pharmaceutical and Clinical Research 15, № 6 (2023): 1391–401. https://doi.org/10.5281/zenodo.12510419.

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<strong>Aim: </strong>Pseudomonas aeruginosa is a gram negative bacillus that produces metallo-β-lactamases (MBLs) and causes several nosocomial infections. The present study was carried to identify such MBL-producing strains in patients. <strong>Methods: </strong>From March 2021 to February 2022, the current study was conducted at the Darbhanga Medical College and Hospital in Laheriasarai, Bihar. The clinical isolates and the MBL prevalence was detected using 3 phenotypic methods namely Combined disc test, Double disk synergy test, and Modified Hodge test. <strong>Res

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Rafati Zomorodi, A., M. Rad, Gh R. Hashemitabar, and H. Salimizand. "Molecular typing of cephalosporin resistant serovars of Salmonella enterica from poultry and farm animals." BULGARIAN JOURNAL OF VETERINARY MEDICINE 23, no. 2 (2020): 178–86. http://dx.doi.org/10.15547/bjvm.2196.

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In this study, the susceptibility to cephalosporins of 74 Salmonella isolates, including 16 isolates from farm animals and 58 isolates from poultry was assessed by the disc diffusion method. ESBL production was evaluated by combined disc diffusion method (CDDM) and double disc synergy test (DDST). The genetic relatedness of isolates was investigated by the Rep-PCR method. The highest prevalence of resistance was observed against cefotaxime (27%) and the least – against cefixime (4%). None of the isolates was ESBL positive. The Rep-PCR generated 54 reproducible fingerprint patterns for all isol

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Çeliksöz, Canan, Tekin Karslıgil, and İclal Balcı. "Extended Spectrum Beta Lactamase Frequency In Cefhtazidime Resistant Pseudomonas Aeruginosa Strains." European Journal of Therapeutics 15, no. 1 (2009): 20–23. http://dx.doi.org/10.58600/eurjther.2009-15-1-1246-arch.

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One of the resistance mechanisms of Pseudomonas aeruginosa, which is an increasing resistance problem to antibiotics, is beta lactamase production. Especially it becomes more and more difficult to apply a treatment to the infections developed by extended spectrum beta lactamase (ESBL) producing strains. It is of importance for the success of the treatment that ESBL is determined in P. aeruginosa and reported to the clinician. In recent years, some ESBL types such as PER-1 in class A and OXA group in class have been determined in P. aeruginosa. These enzymes hydrolyze third generation cefhalosp

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Egbule, O. S., and E. E. Odih. "Prevalence of Extended-Spectrum Beta-Lactamases (ESBLs) and Metallo-BetaLactamases (MBLs) Among Healthy and Hospitalized Children in Abraka and Eku Communities, Delta State, Nigeria." Nigerian Journal of Basic and Applied Sciences 28, no. 1 (2021): 7–14. http://dx.doi.org/10.4314/njbas.v28i1.2.

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This study was conducted to detect the prevalence of Metallo-Beta-Lactamases (MBLs) and Extended Spectrum Beta-Lactamases (ESBLs) in bacterial isolates from clinical and community settings of two communities in Delta State, Nigeria. Eighty four isolates obtained from blood, urine, wound and stool of patients and community subjects were analyzed and identified by standard microbiological methods. Carbapenemase detection was carried out using modified Hodge test. EDTA-disc synergy test was used todetect MBLs production in 26.2% of the isolates. ESBLs production determined by double disc synergy

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Mohammed, Aisha, A. M. Magashi, and M. Yushau. "PHENOTYPIC DETECTION AND ANTIMICROBIAL SUSCEPTIBILITY PROFILE OF EXTENDED SPECTRUM BETA LACTAMASE PRODUCING Escherichia coli FROM SUSPECTED CASES OF URINARY TRACT INFECTION IN KANO METROPOLIS, NIGERIA." FUDMA JOURNAL OF SCIENCES 8, no. 2 (2024): 101–5. http://dx.doi.org/10.33003/fjs-2024-0802-2344.

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Urinary tract infections (UTIs) caused by antimicrobial-resistant bacteria, especially ESBL-producing Escherichia coli can be life-threatening as therapeutic options available to treat infected patients are limited. Resistance due to ESBL-producing bacteria poses a peculiar challenge in treating infections because of its association with multidrug resistance. The aim of this study was thus to determine the susceptibility pattern and phenotypic detection of ESBL-producing E. coli from UTI patients. Two hundred and forty-six (246) E.coli isolates obtained from patients with suspected urinary tra

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Rahman, Nawshad Muhammad Wahidur, Afzalunnessa Binte Lutfor, Sanya Tahmina Jhora, Mahmuda Yasmin, and Jalaluddin Ashraful Haq. "Detection of CTX-M gene in extended spectrum beta lactamase (ESBL) producing Escherichia coli and Klebsiella species of different hospitals." Bangladesh Journal of Medical Microbiology 4, no. 2 (2012): 28–31. http://dx.doi.org/10.3329/bjmm.v4i2.10829.

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A total of 200, non-duplicate ESBL producing strains (171 Escherichia coli and 29 Klebsiella spp.) from three tertiary care hospitals were detected using screening test & double disc synergy test. All isolates were screened for the detection of CTX-M type Extended spectrum Beta-lactamase (ESBL) using PCR. Among them 133 (66.5%) were positive for CTX-M type ESBLs which include 114 (66.66%) E.coli and 19 (65.51%) Klebsiella spp. This is the first report of identifying CTX-M gene in ESBL producing Escherichia coli and Klebsiella species of different hospitals.DOI: http://dx.doi.org/10.3329/bj

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C., C. Ezeanya, R. Agbakoba N., E. Ejike C., and I. Okwelogu S. "Evaluation of a Chromogenic Medium for the Detection of ESBL with Comparison to Double Disk Synergy Test." British Journal of Medicine & Medical Research 21, no. 12 (2017): 1–11. https://doi.org/10.9734/BJMMR/2017/33259.

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<strong>Background:</strong> Extended Spectrum Beta Lactamase (ESBL) producing bacterial strains are the major causes of nosocomial and community-acquired infections worldwide. The aim of the study was to evaluate the effectiveness of Brilliance ESBL Agar (BEA) (a chromogenic culture medium) for the detection of ESBL in comparison with Double Disc Synergy Test (DDST) and confirm results from both methods by Single-plex Polymerase Chain Reaction (PCR) as gold standard. <strong>Materials and Methods:</strong> A total of 75 clinical isolates of <em>Escherichia coli</em> were screened for ESBL pro

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Taqdees, Malik, Asma Naim, and Asma Saeed. "Identification and Antibiotic Susceptibility Testing of ESBL producing Klebsiella strains by Phenotypic methods." RADS Journal of Biological Research & Applied Sciences 9, no. 1 (2018): 8–13. http://dx.doi.org/10.37962/jbas.v9i1.147.

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Multi drug resistance has now become a worldwide therapeutic challenge due to the widespread use of broad spectrum antibiotics. Klebsiella species have significant importance in clinical field as they cause various infections in human and are considered as potential pathogens that express antibiotic resistance through their strong enzymatic activity. Extended spectrum beta lactamases (ESBLs) are plasmid mediated enzymes produced mostly because of mutation and few other factors. These enzymes confer resistance against various Î²-lactam drugs including cephalosporins and monobactams. Among the g

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O, Ikwuagwu, Vivian, Akujobi C. O, Chinakwe E. C, Ekeleme U. G, Ugenyi L. C, and Ohaeri E. "Antimicrobial Activity of Pterocarpus santalinoides, Crescentia cujete, and Azadirachta indica Leaf Extracts on Extended Spectrum Beta Lactamases (ESBL) Producing Escherichia coli." Microbiology Research Journal International 35, no. 6 (2025): 1–15. https://doi.org/10.9734/mrji/2025/v35i61581.

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Aim: The study investigates the antimicrobial activity of three selected plant leaf extracts—Pterocarpus santalinoides, Crescentia cujete, and Azadirachta indica on extended-spectrum beta-lactamase (ESBL) producing E. coli in clinical isolates from wound and stool specimens. Study Area: Clinical specimens, including stool and wound swabs, were collected from patients at Gregory University Clinics, Uturu. Study Design and Methodology: Standard microbiological methods were employed for bacterial isolation and identification. ESBL-producing isolates were identified using screening and phenotypic

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Mohammed, A., A. M. Magashi, and M. Yushau. "Incidence of extended spectrum Beta-Lactamase producing Klebsiella pneumoniae among patients with urinary tract infections in Kano Metropolis Nigeria." Bayero Journal of Pure and Applied Sciences 12, no. 1 (2020): 139–44. http://dx.doi.org/10.4314/bajopas.v12i1.23s.

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Extended Spectrum Beta-Lactamase (ESBLs) production is one of the ways by which bacteria become resistant to antibiotics and pathogens of UTIs such as Klebsiella pneumoniae have been incriminated at global scale. This study was conducted to investigate the incidence of Extended Spectrum Beta lactamase producing Klebsiella pneumoniae from Urinary Tract Infections in Kano metropolis. The work involved One hundred and fourty seven K. Pneumoniae isolates obtained from patients with suspected urinary tract infections were studied from January to July 2017. The identity of the isolates was confirmed

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Afrin, Dilruba, Milad Ahmed, Anik Banik, et al. "Phenotypic screening of extended-spectrum beta-lactamase-producing Salmonella in retail shrimp." Journal of Advanced Biotechnology and Experimental Therapeutics 6, no. 3 (2023): 686. http://dx.doi.org/10.5455/jabet.2023.d159.

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The occurrence of extended-spectrum beta-lactamase (ESBL) producing bacteria is concerning the scientific community since it confers multiple drug resistance (MDR). The study investigated the prevalence of MDR-ESBL-producing Salmonella strains from shrimp in Sylhet region, Bangladesh. A total of 165 shrimp samples were processed from 55 shrimp specimen from different retail shops. The presence of Salmonella was confirmed by standard methods followed by antibiotic susceptibility testing. Isolates exhibiting resistant to third-generation cephalosporin were considered as Salmonella positive isola

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Fathi Azar Khavarani, Motahareh, Mahla Najafi, Zahra Shakibapour, and Davood Zaeifi. "Kinetics activity of Yersinia Intermedia Against ZnO Nanoparticles Either Synergism Antibiotics by Double-Disc Synergy Test Method." Iranian Journal of Biotechnology 14, no. 1 (2016): 39–44. http://dx.doi.org/10.15171/ijb.1184.

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Kabir, Rubaiya Binte, Mohammad Jobayer, Nasreen Farhana, and S. M. Shamsuzaman. "Prevalence of extended spectrum beta-lactamase and metallo-beta-lactamase genes in imipenem resistant Pseudomonas aeruginosa from admitted patients in a tertiary level hospital, Bangladesh." International Journal of Research in Medical Sciences 12, no. 7 (2024): 2258–65. http://dx.doi.org/10.18203/2320-6012.ijrms20241869.

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Background: Pseudomonas aeruginosa are known for their multiple mutations and rapid acquirement of antimicrobial resistance genes. The presence of metallo-β-lactamase (MBL) is the commonest reason for the treatment failure in carbapenem therapy. Production of extended spectrum β-lactamase (ESBL) in these isolates makes the treatment more challenging. Due to the importance of the carbapenems in resistant infection management, finding the true frequencies of such enzymes is imperative. Methods: A total of 446 samples were collected from the admitted patients with infected burn, surgical wound, a

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Hager, Raghda, та Bassant M. Sayed. "Comparative Study of Phenotypic Detection Methods of Metallo-beta-lactamase (MβL) Among Carbapenem Resistant Gram-Negative Bacilli (GNB) Isolated from Intensive Care Units (IUC) Patients". Egyptian Journal of Medical Microbiology 29, № 3 (2020): 75–80. http://dx.doi.org/10.51429/ejmm29310.

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Background: Metallo-beta-lactamase (MβL) mediated resistance is an emergency threat in health care settings, and its identification is essential for treatment and infection control. Objectives: this study aimed to detect the MβL prevalence in Gram negative bacilli (GNB) and to compare its phenotypic detection methods. Methodology: Ninety six (96) isolates of GNB were isolated from different clinical specimens collected from patients admitted to intensive care units (ICU) of Ain Shams Hospitals, from February 2018 to June 2018. Isolates were screened for carbapenem resistance with imipenem 10 µ

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Mahalle, Rashmi. "TO CORRELATE DOUBLE DISC SYNERGY TEST (DDST) AND ETEST RESULTS FOR ESBL DETECTION IN ECOLI & KLEBSIELLA PNEUMONIAE ISOLATES." Journal of Evolution of Medical and Dental Sciences 4, no. 62 (2015): 10808–12. http://dx.doi.org/10.14260/jemds/2015/1560.

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Syah Putra, Akyun Rozaqi, Mustofa Helmi Effendi, Setiawan Koesdarto, Suwarno Suwarno, Wiwik Tyasningsih та Agnes Theresia Soelih Estoepangestie. "IDENTIFIKASI BAKTERI Escherichia coli PENGHASIL Extended Spectrum β-Lactamase DARI SWAB RECTAL SAPI PERAH MENGGUNAKAN METODE VITEK-2 DI KUD TANI WILIS SENDANG KABUPATEN TULUNGAGUNG". Journal of Basic Medical Veterinary 8, № 2 (2020): 108. http://dx.doi.org/10.20473/.v8i2.20414.

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Antibiotic resistance in animals and humans has become a global problem that needs attention. The use of antibiotics in inappropriate on food-producing animals can lead to resistance many of the pathogenic bacteria to the various types of antibiotics, one of which is the Escherichia coli (E. coli) which produces extended spectrum β-lactamase (ESBL). The aim of this study was to isolate and identified ESBL- E. coli isolate from dairy cow rectal swabs in Sendang, Tulungagung district using the Vitek-2 method. The number of rectal swab samples used in the present study was 50. The result of the s

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Ramanath, K., Munesh K. Sharma, Madhurendra S. Rajput, Navdeep, Sandeep Negi, and Viraj Bhate. "An Approach of Phenotypic Detection of Metallo-Beta Lactamase Producing Pseudomonas aeruginosa: A Study in Madhya Pradesh." Medical Journal of Dr. D.Y. Patil Vidyapeeth 16, no. 3 (2023): 403–9. http://dx.doi.org/10.4103/mjdrdypu.mjdrdypu_164_21.

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ABSTRACT Introduction: Antibiotic resistance in the last decade has recorded drastic changes in an increasing manner in nonfermenting bacterial groups of gram-negative bacilli including Ps. aeruginosa is a potent bacterial isolate or opportunistic nosocomial human pathogen. In recent decades, with improvements in antimicrobial therapy and diagnostic procedures have emerged multidrug resistant with beta lactamase to β-lactam antibiotics in presence of carbapenemases group of enzymes as metallo β-lactamases (MBL) are a major cause of concern. Due to its transferable character, it can hydrolyze a

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Sajjad, Ayesha, Muna Malik, Iffat Javed, Sohaila Mushtaq, Fareeha Imran, and Rabiya Jameel. "Detection of metallo beta lactamase production in imipenem resistant gram negative bacilli non fermenters isolated in a Tertiary Care Hospital." Professional Medical Journal 26, no. 12 (2019): 2080–84. http://dx.doi.org/10.29309/tpmj/2019.26.12.3073.

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Objectives: Metallo-beta lactamase (MBL) producing non fermenter Gram negative bacilli is an emerging warning and cause of worry as they have established as one of the most feared resistance mechanisms and are the foremost cause of nosocomial infections worldwide. Carbapenem, including Imipenem, Meropenem and Doripenem are often used as a last remedy for treatment of infections caused by Pseudomonas aeruginosa, Acinetobacter and other Gram-negative. The present study was designed to explore the distribution of imipenem resistant non-fermenter Gram-negative bacilli isolates in different age gro

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Fallah, Mahnaz Shafaei, Hadi Razavi Nikoo, Aylar Jamali, Alireza Mohebbi, and Ezzat Allah Ghaemi. "A cross-sectional study on the prevalence of multidrug-resistant clinical isolates of Proteus species in Northern Iran." Journal of Infection in Developing Countries 18, no. 12 (2024): 1855–60. https://doi.org/10.3855/jidc.17637.

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Introduction: Multidrug-resistant (MDR) bacteria like Proteus species have led to more prolonged hospitalizations, fewer care choices, higher treatment costs, and even death. The present study aims to evaluate the prevalence of MDR Proteus species in clinical samples and to suggest the best therapeutic options for the MDR Proteus species. Methodology: Clinical samples were collected randomly from five hospitals in Golestan Province, Iran, from February 2017 to July 2019. Disk diffusion on Mueller–Hinton agar plates were used to perform antibiotic susceptibility testing (ASTs). By using a doubl

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Pehlivanoglu, Faruk, and Ezgi Sababoglu. "Characterisation of AmpC / ESBL genes in some pathogen gram-negatives isolated from clinical cases of livestock and companion animals." Acta Veterinaria 71, no. 4 (2021): 435–50. http://dx.doi.org/10.2478/acve-2021-0036.

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Abstract This study was aimed to search and characterize the AmpC and/or ESBL genes of Escherichia coli, Klebsiella pneumoniae and Pseudomonas aeruginosa isolated from clinical cases of local livestock and companion animals between 2017 and 2019. A total of eight ceftiofur-resistant E. coli (n= 7) and ceftiofur-resistant K. pneumoniae (n= 1) and seven P. aeruginosa were isolated from different cases in local animals. By combination disc method, six E. coli isolates and one K. pneumoniae isolate were found to be ESBL producers. By combination of the disc method and double disc synergy test, no

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Neetu Parmar, Amit Tiwari та Arjun Singh Parmar. "Prevalence of extended spectrum β-lactamase (ESBL) and AmpC β-lactamase producing bacteria in urinary tract infection patients visiting tertiary care hospital in Central India". World Journal of Biology Pharmacy and Health Sciences 20, № 3 (2024): 670–76. https://doi.org/10.30574/wjbphs.2024.20.3.1078.

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Antimicrobial resistance showed by different uropathogens is one of the barricades that might hinder the successful treatment (Bajpai et al., 2019). Detection of extended beta–lactamase (ESBL) production among uropathogens is an important marker of endemicity. Aim: This study was undertaken to determine the prevalence of pathogens in urinary tract and their antimicrobial susceptibilities, based on extended-spectrum beta-lactamase (ESBL) and AmpC beta-lactamase production in Central India. Methodology and results: The prevalence of pathogenic microorganisms in urinary tract and their antimicrob

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Dashti, Ali A., Mehrez M. Jadaon, Huda H. Gomaa, Bobby Noronha, and Edet E. Udo. "Transmission of a Klebsiella pneumoniae clone harbouring genes for CTX-M-15-like and SHV-112 enzymes in a neonatal intensive care unit of a Kuwaiti hospital." Journal of Medical Microbiology 59, no. 6 (2010): 687–92. http://dx.doi.org/10.1099/jmm.0.019208-0.

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The spread of antibiotic-resistant bacteria has become a large problem in most countries including Kuwait. This antibiotic resistance is usually due to the production of extended-spectrum β-lactamase (ESBL) enzymes such as SHV, TEM and CTX-M. This study reports the emergence and spread of an ESBL-producing Klebsiella pneumoniae clone in a neonatal intensive care unit (NICU) in a Kuwaiti hospital. Eight ESBL-producing K. pneumoniae isolates were from blood cultures of seven neonates, and two were from the fingers of two healthcare workers in a NICU in Al Jahra Hospital, Kuwait. All isolates wer

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Goudarzi, Mehdi, Mehdi Azad та Sima Sadat Seyedjavadi. "Prevalence of Plasmid-Mediated Quinolone Resistance Determinants and OqxAB Efflux Pumps among Extended-Spectrumβ-Lactamase ProducingKlebsiella pneumoniaeIsolated from Patients with Nosocomial Urinary Tract Infection in Tehran, Iran". Scientifica 2015 (2015): 1–7. http://dx.doi.org/10.1155/2015/518167.

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Objective. Plasmid-mediated quinolone resistance (PMQR) plays an important role in the development of clinical resistance to quinolone. The aim of this study was to investigate PMQR determinants among extended-spectrumβ-lactamases- (ESBL-) producingKlebsiella pneumoniaerecovered from patients with nosocomial urinary tract infection (UTI).Methods. A total of 247 ESBL-producingK. pneumoniaeisolates were collected from 750 patients with UTI. ESBL production was confirmed by double disc synergy test and combined disc diffusion test. The prevalence of PMQR determinants among ESBL-producingK. pneumo

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Anandkumar, H., Amaresh Nigudgi, Vinay Hajare, and Sunil Biradar. "Evaluation of different phenotypic diffusion methods in the identification of extended spectrum beta lactamase producing uropathogenic." Indian Journal of Microbiology Research 8, no. 3 (2021): 243–48. http://dx.doi.org/10.18231/j.ijmr.2021.050.

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: The study was aimed to identify the occurrence of extended spectrum of Beta lactamases (ESBLs), to compare different phenotypic methods used for the confirmation and to evaluate the antibiotic resistance pattern in ESBL producing uropathogenic : The strains were isolated from urine and the isolates resistance to at least one of the three representative cephalosporins (cefotaxime, cefpodoxime and ceftazidime) was tested for ESBL production by Double disc synergy test (DDST), Inhibitory potentiated disc diffusion (IPDD) test and quantitative E-strip method.: Of 120strains isolated, 62(51.6%) w

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Giriyapur, Ravi S., Namratha W. Nandihal, Krishna B. V. S., Asha B. Patil, and Chandrasekhar M. R. "Comparison of Disc Diffusion Methods for the Detection of Extended-Spectrum Beta Lactamase-Producing Enterobacteriaceae." Journal of Laboratory Physicians 3, no. 01 (2011): 033–36. http://dx.doi.org/10.4103/0974-2727.78561.

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ABSTRACTBackground: Resistance to broad-spectrum β lactams, mediated by extended-spectrum β lactamases (ESBLs), is an increasing problem world wide. This resistance poses problems for in vitro testing and reporting. Increased prevalence of ESBLs among Enterobacteriaceae creates a great need for laboratory testing methods that will accurately identify their presence.Materials and Methods: During the study, the Enterobacteriaceae isolated were tested for the presence of ESBL by the National Committee for Clinical Laboratory Standards (NCCLS) screening test, Jarlier double disc synergy (approxima

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Saha, Mili Rani, and Sanya Tahmina Jhora. "Detection of extended spectrum beta-lactamase producing Gram-negative organisms: hospital prevalence and comparison of double disc synergy and E-test methods." IMC Journal of Medical Science 12, no. 1 (2018): 32–36. http://dx.doi.org/10.3329/imcjms.v12i1.35176.

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Background and objectives: Emergence of extended spectrum beta-lactamase (ESBL) producing bacteria is a major public health concern. Detection of multi drug resistant (MDR) ESBL producing organisms is necessary to prevent its spread and effective treatment. The purpose of the present study was to determine the magnitude of ESBL producing organism in hospital setting and to compare the suitability of double disc synergy test (DDST) and cefepime-clavulanate E-test method for the detection of ESBL producing organisms in routine microbiology laboratory.Materials and methods: The study was carried

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Khan, Shah Muhammad, Amjad Ali, Qazi Ikramullah, Ali Zaman, Tariq Mehmood, and Samina Jadoon. "Incidence of multidrug resistance and extended spectrum beta-lactamase expression in community-acquired urinary tract infection among different age groups of patients." International journal of health sciences 7, S1 (2023): 25–32. http://dx.doi.org/10.53730/ijhs.v7ns1.14147.

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Background and Aim: Escherichia coli was the most prevalent cause for urinary tract infections (UTIs). These bacteria also generate extended spectrum b-lactamases (ESBL), which inactivate penicillins and cephalosporins. The purpose of the current study is to determine the incidence of multidrug resistance and extended beta-lactamase expression in community-acquired urinary tract infection (UTIs) among patients of different age groups. Patients and Methods: This prospective cross-sectional study was conducted on 186 uropathogens investigated in the diagnostic center of Tertiary Care Hospital Pe

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Qasim, M., U. U. Rehman, Saman, et al. "CURRENT PREVALENCERATE OF ESBL PRODUCTION IN SALMONELLA TYPHI AMONG MULTIDRUG-RESISTANT (MDR) STRAINS AT CITI LAB AND RESEARCH CENTER (CRC)." Pakistan Journal of Science 76, no. 04 (2024): 584–87. https://doi.org/10.57041/vol76iss04pp584-587.

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Background: This study is conducted to establish the epidemiology ofrate of occurrence of extended-spectrum β-lactamase (ESBL) production among multidrug-resistant (MDR) Salmonella typhi isolates during the whole period of the year. Methods: Among the 327 blood cultures samples 153 found to be MDR S.typhi isolates. For the identification of isolates API-20 E system was used, and Kirby-Bauer method of disc diffusion for antibiotic sensitivitywas applied. Double-disc synergy test was performed to check ESBL-production. Results: Susceptibility among anti-typhoid drugs of first-line was as follows

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Qasim, M., U. U. Rehman, Saman, et al. "CURRENT PREVALENCERATE OF ESBL PRODUCTION IN SALMONELLA TYPHI AMONG MULTIDRUG-RESISTANT (MDR) STRAINS AT CITI LAB AND RESEARCH CENTER (CRC)." Pakistan Journal of Science 76, no. 04 (2024): 584–88. https://doi.org/10.57041/vol76iss04pp584-588.

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Background: This study is conducted to establish the epidemiology ofrate of occurrence of extended-spectrum β-lactamase (ESBL) production among multidrug-resistant (MDR) Salmonella typhi isolates during the whole period of the year. Methods: Among the 327 blood cultures samples 153 found to be MDR S.typhi isolates. For the identification of isolates API-20 E system was used, and Kirby-Bauer method of disc diffusion for antibiotic sensitivitywas applied. Double-disc synergy test was performed to check ESBL-production. Results: Susceptibility among anti-typhoid drugs of first-line was as follows

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Swanson, K. J., D. Nault, G. Demessie, S. Mendoza, R. Yee, and P. Nagao. "Detecting and Reporting Extended Spectrum Beta-Lactamases Producers: Evaluation of Three Approaches and the Clinical Impact." American Journal of Clinical Pathology 162, Supplement_1 (2024): S114—S115. http://dx.doi.org/10.1093/ajcp/aqae129.254.

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Abstract Introduction/Objective Since the COVID-19 pandemic, bacteria producing extended spectrum beta-lactamases (ESBLs) have increased &gt;30%. Laboratories can perform phenotypic microbroth dilution or molecular approaches to identify ESBL producers. However, the gold standard is a laborious double disc synergy test. We evaluated the concordance among the three approaches, the clinical impact, and justification of reporting ESBL producers. Methods/Case Report Seventy-eight ESBL producers identified by microbroth dilution (MicroScan WalkAway, Beckman Coulter, Brea, California, USA) or de

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