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Artykuły w czasopismach na temat „Extended spectrum beta lactamase genes”

Autor: Grafiati

Data publikacji: 3 czerwca 2025

Data aktualizacji: 31 lipca 2025

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1

Dikoumba, Anicet-Clotaire, Pierre Philippe Mbehang Nguema, Leresche Even Doneilly Oyaba Yinda, et al. "Characterization of Extended-Spectrum Beta-Lactamase-Producing Escherichia coli in Diarrhoeal Faeces from 0 to 5-Year-Old Children Attending Public Hospitals in Franceville, Gabon." Antibiotics 13, no. 11 (2024): 1059. http://dx.doi.org/10.3390/antibiotics13111059.

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Background: In Gabon, studies on the characterization of extended-spectrum beta-lactamase-producing Escherichia coli in young children with diarrhoea are almost nonexistent. The objective was to evaluate the prevalence of antibiotic resistance to extended-spectrum beta-lactamase-producing Escherichia coli in children at public hospitals in Franceville, Gabon. Methods: Seventy diarrhoea faecal samples were collected from children aged 0–5 years. The culture and isolation of colonies were carried out on MacConkey agar. The colonies were identified using VITEK 2. The determination of the extended

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Szabó, Dóra, Melissa A. Melan, Andrea M. Hujer, et al. "Molecular Analysis of the Simultaneous Production of Two SHV-Type Extended-Spectrum Beta-Lactamases in a Clinical Isolate of Enterobacter cloacae by Using Single-Nucleotide Polymorphism Genotyping." Antimicrobial Agents and Chemotherapy 49, no. 11 (2005): 4716–20. http://dx.doi.org/10.1128/aac.49.11.4716-4720.2005.

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ABSTRACT Bacteria that simultaneously produce multiple extended-spectrum beta-lactamases are frequently isolated. We report an Enterobacter cloacae isolate, ES24, producing four different beta-lactamases (AmpC type beta-lactamase, TEM-1, SHV-7, and a novel extended-spectrum beta-lactamase, SHV-30). Direct sequencing of bla SHV gene products gave a “double peak” at position 703, suggesting the presence of more than one allele. Using fluorescence resonance energy transfer real-time PCR to detect single-nucleotide polymorphisms, we were able to distinguish two different bla SHV genes in a single

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Anjelina, Liya, and Armen Ahmad. "Diagnosis and Treatment-Spectrum Beta-Lactamase and Multidrug Resistance Bacterial." Bioscientia Medicina : Journal of Biomedicine and Translational Research 6, no. 10 (2022): 221–2230. http://dx.doi.org/10.37275/bsm.v6i10.581.

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Multidrug-resistant (MDR) is a condition resistant to at least one type of antibiotic from 3 classes of antibiotics. Extended-Spectrum Beta Lactamases are globular proteins that consist of alpha-helices and beta-pleated sheets. β-lactamase hydrolyze broad-spectrum cephalosporin with oxyimino side chain. ESBL hydrolyze antibiotics group penicillin, cephalosporin first, second, third, fourth generation, and monobactam aztreonam. Multidrug resistance occurs through two mechanisms, bacteria accumulate multiple genes encoding resistance to one antibiotic, and due to increased expression of genes en

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. AL-Kaisse, Asmaa A., Amina N. AL-Thwani, and Rabab Q. AL-Segar. "PCR Detection of Some ESBLs (bla) Genes in Pseudomonas aeruginosa Isolated from Burn’s Units in Bagdad Hospitals." Journal of Biotechnology Research Center 9, no. 2 (2015): 74–80. http://dx.doi.org/10.24126/jobrc.2015.9.2.439.

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Multi drug resistant (MDR) and Extended Spectrum Beta Lactamase (ESBLs) of Pseudomonas aeruginosawere detected. Pseudomonas aeruginosa is a bacterium responsible for severe infections in burn’s units,plasmid DNA analysis and encoded many types of genes responsible for beta-lactamases. To determine thetype of genes responsible for beta-lactam broad spectrum in P. aeruginosa strains isolated from 100 swabsof burn’s units environment, using a molecular methods (PCR) by primers specific to ESBLs (bla ) genesoxacillin hydrolyzing capabilities OXA-10, OXA-4 and Vietnam Extended-Spectrum β-Lactame VE

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Fournier, B., and P. H. Roy. "Variability of chromosomally encoded beta-lactamases from Klebsiella oxytoca." Antimicrobial Agents and Chemotherapy 41, no. 8 (1997): 1641–48. http://dx.doi.org/10.1128/aac.41.8.1641.

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The beta-lactamase genes of Klebsiella oxytoca were previously divided into two main groups: bla(OXY-1) and bla(OXY-2). The two beta-lactamase groups were each represented by beta-lactamases with four different pIs. In each group, one form of beta-lactamase is more frequent than the others combined. The beta-lactamase gene of each representative beta-lactamase with a different pI that was not yet sequenced (pIs 5.7, 6.8 [OXY-2], 7.1, 8.2, and 8.8 [OXY-1]) was cloned and sequenced. The susceptibility patterns as well as relative rates and kinetic parameters for beta-lactam hydrolysis revealed t

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Nerway, Sozan M., and Muna S. Al-Delaimi. "Phenotypic and Molecular Detection of Extended Spectrum Beta-Lactamases Producing-Bacteria Isolated From Pregnant Women With Genital Tract Infection in Duhok Governorate." Science Journal of University of Zakho 9, no. 2 (2021): 80–88. http://dx.doi.org/10.25271/sjuoz.2021.9.2.784.

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Beta-lactamase producing bacteria have a worldwide distribution with a high degree of prevalence in both community and hospital. Furthermore, multidrug resistant (MDR) and extended spectrum β-lactamases (ESBL) producing bacterial isolates from women patients may limit treatment options available. This study was designed to determine the frequency of bacterial isolates associated with genital tract infection in pregnant women and their antimicrobial resistance profile and to assess the prevalence of extended spectrum β-lactamases producing bacteria. Demonstrating the β-lactamase genes (blaTEM,

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Sulian, O. S., V. A. Ageevets, A. A. Sukhinin, et al. "Associated resistance of Escherichia coli isolated from humans and animals to polymyxin and beta-lactam antibiotics." Antibiotics and Chemotherapy 66, no. 11-12 (2022): 9–17. http://dx.doi.org/10.37489/0235-2990-2021-66-11-12-9-17.

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Escherichia coli isolates from various sources from 2018 to 2019 were included in the study. Mcr-1 genes were found in two of 105 animal strains (2%) and seven of 928 human strains (0.8%). All mcr-1-positive strains showed a low level of resistance to colistin (MIC ranged from 4 to 8 µg/ml). Both strains isolated from animals remained sensitive to betalactam antibiotics and did not contain beta-lactamase genes. Beta-lactamases were absent only in one of the strains isolated from humans. Four strains were resistant to cephalosporins with sensitivity to carbapenems and carried class A (blaCTX-M-

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Beshah, Daniel, Adey Feleke Desta, Gurja Belay Woldemichael, et al. "High burden of ESBL and carbapenemase-producing gram-negative bacteria in bloodstream infection patients at a tertiary care hospital in Addis Ababa, Ethiopia." PLOS ONE 18, no. 6 (2023): e0287453. http://dx.doi.org/10.1371/journal.pone.0287453.

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Background Bloodstream infection due to beta-lactamase and carbapenemase-producing gram-negative bacteria poses a substantial challenge to the effectiveness of antimicrobial treatments. Therefore, this study aimed to investigate the magnitude of beta-lactamase, carbapenemase-producing gram-negative bacteria, and associated risk factors of bloodstream infections in patients at a tertiary care hospital, in Addis Ababa, Ethiopia. Methods An institutional-based cross-sectional study was conducted with convenience sampling techniques from September 2018 to March 2019. Blood cultures were analyzed f

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Fournier, B., P. H. Roy, P. H. Lagrange, and A. Philippon. "Chromosomal beta-lactamase genes of Klebsiella oxytoca are divided into two main groups, blaOXY-1 and blaOXY-2." Antimicrobial Agents and Chemotherapy 40, no. 2 (1996): 454–59. http://dx.doi.org/10.1128/aac.40.2.454.

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The chromosomally encoded beta-lactamase gene (blaOXY-2) of the wild-type Klebsiella oxytoca SL911 was cloned and sequenced. Its nucleotide sequence similarity with the previously sequenced K. oxytoca beta-lactamase gene (blaOXY-1) (Y. Arakawa, M. Ohta, N. Kido, M. Mori, H. Ito, T. Komatsu, Y. Fujii, and N. Kato, Antimicrob. Agents Chemother. 33:63-70, 1989) is 87.3%, and its amino acid similarity is 89.7%. This group of K. oxytoca beta-lactamases is related to chromosomal beta-lactamases of Citrobacter diversus, Proteus vulgaris, and Yersinia enterocolitica and to the plasmid-mediated extende

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Owusu, Felicia A., Noah Obeng-Nkrumah, Esther Gyinae, et al. "Occurrence of Carbapenemases, Extended-Spectrum Beta-Lactamases and AmpCs among Beta-Lactamase-Producing Gram-Negative Bacteria from Clinical Sources in Accra, Ghana." Antibiotics 12, no. 6 (2023): 1016. http://dx.doi.org/10.3390/antibiotics12061016.

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Beta-lactamase (β-lactamase)-producing Gram-negative bacteria (GNB) are of public health concern due to their resistance to routine antimicrobials. We investigated the antimicrobial resistance and occurrence of carbapenemases, extended-spectrum β-lactamases (ESBLs) and AmpCs among GNB from clinical sources. GNB were identified using matrix-assisted laser desorption/ionization time of flight–mass spectrometry (MALDITOF-MS). Antimicrobial susceptibility testing was performed via Kirby–Bauer disk diffusion and a microscan autoSCAN system. β-lactamase genes were determined via multiplex polymerase

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Kumar, Diwakar, Ananya Choudhury, Mitul Nath, and Udaya Kumar Vandana. "SCREENING FOR EXTENDED-SPECTRUM BETA-LACTAMASES PRODUCING ESCHERICHIA COLI ISOLATES IN FISH SAMPLE AND PROFILING FOR ANTIBIOTICS SUSCEPTIBILITY." Bacterial Empire 4, no. 1 (2021): 25–29. http://dx.doi.org/10.36547/be.2021.4.1.25-29.

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At present, extended-spectrum β-lactamase (ESBL)-producing Escherichia coli and other species of Enterobacteriaceae have emerged as a matter of utmost concern. Indiscriminate utilization of antimicrobials in aquaculture generates selective pressure creating reservoirs of drug-resistance genes in fish pathogens and other bacteria, which may disseminate by horizontal gene transfer and reach human pathogens. The present study aims to detect extended-spectrum beta-lactamases (ESBL) production by enterobacteria isolated from locally harbored and imported fresh and dry fishes. Out of 235 fish sample

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Peretyatko, O. G., Yu. A. Yagnyuk, N. I. Sklyar, G. M. Bolshakova, and T. V. Kholodna. "Beta-lactamases of enterobacteria: general characteristics, mechanisms and regional features of distribution." Annals of Mechnikov Institute, no. 3 (September 12, 2022): 7–12. https://doi.org/10.5281/zenodo.7070850.

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The problem of the formation and spread of resistance to β-lactam antibiotics in clinically significant types of microorganisms is extremely important, since β-lactams traditionally form the basis of the treatment of bacterial infections. Special attention of antibiotic resistance researchers is directed to the microorganisms of the Enterobacteriaceae family, namely, to pathogens such as <em>Enterobacter spp., Citrobacter spp., Proteus spp., K. pneumoniae, E. coli,</em> which are capable of producing a wide range of β-lactamases. Since the discovery of the first beta-lactamase i

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SADEGHI DEYLAMDEH, Zahra, and Abolfazl JAFARI SALES. "Evaluation of the presence of AmpC (FOX) beta-lactamase gene in clinical strains of Escherichia coli isolated from hospitalized patients in Tabriz, Iran." Journal of Experimental and Clinical Medicine 38, no. 3 (2021): 301–4. http://dx.doi.org/10.52142/omujecm.38.3.17.

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Beta-lactamases are the most common cause of bacterial resistance to beta-lactam antibiotics. AmpC-type beta-lactamases hydrolyze cephalosporins, penicillins, and cephamycins. Therefore, the study aims was to determine antibiotic resistance and to investigate the presence of AmpC beta-lactamase gene in clinical strains of Escherichia coli isolated from hospitalized patients in Tabriz. In this cross-sectional descriptive study, 289 E. coli specimens were collected from clinical specimens. Disk diffusion method and combined disk method were used to determine the phenotype of extended spectrum β-

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Shah, Hamid Ahmad, Dalip K. Kakru, and Mohd Altaf Bhat. "Antimicrobial Gene Profile of Extended Spectrum Beta Lactamases Escherichia coli from Human Clinical Isolates in North India." International Journal of Current Microbiology and Applied Sciences 13, no. 4 (2024): 207–14. http://dx.doi.org/10.20546/ijcmas.2024.1304.023.

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Antimicrobial resistance (AMR) is an extensive public health problem due to the rapid increase in the population of multidrug-resistant bacteria. Escherichia coli, a common bacterial pathogen, has demonstrated resistance to beta-lactam antibiotics through the formation of beta- lactamase enzymes. Understanding the genetic diversity and co-carriages of β-lactamase genes in E. coli is essential for developing effective strategies to combat AMR. In this study, we conducted a comprehensive molecular characterization of beta-lactamase E. coli isolates collected from clinical in the northern Indian

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Schmitt, Joachim, Enno Jacobs, and Herbert Schmidt. "Molecular characterization of extended-spectrum beta-lactamases in Enterobacteriaceae from patients of two hospitals in Saxony, Germany." Journal of Medical Microbiology 56, no. 2 (2007): 241–49. http://dx.doi.org/10.1099/jmm.0.46670-0.

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Between January and September 2003, 39 isolates of the family Enterobacteriaceae with phenotypically positive Vitek 1 extended-spectrum beta-lactamase (ESBL) test results were collected, originating from patients of two hospitals in Saxony, Germany. Plasmid DNA was isolated and screened by PCR for the presence of genes encoding beta-lactamases of SHV, TEM and CTX-M types. To differentiate ESBL and non-ESBL among SHV and TEM genes, detailed analysis of PCR products was performed. Twenty-four strains carried SHV-2, SHV-5 or SHV-12 genes. In a further 11 strains a CTX-M gene was detected. The CTX

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Wolny-Koładka, Katarzyna, and Marek Zdaniewicz. "Antibiotic Resistance of Escherichia coli Isolated from Processing of Brewery Waste with the Addition of Bulking Agents." Sustainability 13, no. 18 (2021): 10174. http://dx.doi.org/10.3390/su131810174.

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The aim of the study was to determine the drug resistance profile and to assess the presence of genes responsible for the production of extended-spectrum beta-lactamases in Escherichia coli isolated from energy-processed hop sediment with the addition of bulking agents. Antibiotic resistance was determined by the disk diffusion method and the PCR technique to detect genes determining the extended-spectrum beta-lactamases (ESBLs) mechanism. A total of 100 strains of E. coli were collected. The highest resistance was found to aztreonam, tetracycline, ampicillin, ticarcillin, and ceftazidime. The

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Prabhurajan, Rajan, Rajasekharapanicker Kiran, and Kesavaram Padmavathy. "Extended Spectrum Beta-lactamase producing Salmonella Lindenberg gastroenteritis." Journal of Infection in Developing Countries 13, no. 09 (2019): 851–53. http://dx.doi.org/10.3855/jidc.11409.

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Introduction: Increasing antimicrobial resistance among non-typhoidal Salmonella (NTS) is a major public health issue especially in developing countries and is partly due to the use of antimicrobials in animal feeds as growth promoters. NTS are often associated with self-limiting acute gastroenteritis (AGE). Nevertheless, fluoroquinolones and third-generation cephalosporins are currently used in the treatment of severe diarrhoeal infections. Methodology: We report the case of a 30-year-old male who presented with clinical symptoms of moderate gastroenteritis. Stool culture and antibiotic

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Le, Kien Chi, Cuong Quoc Vo, Xuan Thanh Tran, et al. "Carriage of ESBL and Amp-C -b -lactamase among Escherichia coli strains isolated from dogs in kennels Dak Lak province." Science and Technology Development Journal - Natural Sciences 5, no. 2 (2021): 1198–207. http://dx.doi.org/10.32508/stdjns.v5i2.996.

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The global prevalence of antimicrobial resistance and Extended-Spectrum and AmpC Beta- Lactamases is continuously widespread among Escherichia coli during recent years, especially in Viet Nam. In Viet Nam, there have been researches on ESBL and AmpC-carrying E. coli inhabiting animal and human. However, studies of antimicrobial resistance in E. coli residing in pets, especially dogs are unavailable. The aim of the study was to investigate the antimicrobial sensitivity testing (AST), the resistance to 3rd cephalosporin and penicillin, also to assess the molecular detection of ESBL and Amp-C-bet

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Ogbolu, David Olusoga, Oluwole Adebayo Daini, Afolabi Ogunledun, Oyebode Armstrong Terry Alli, and Mark Alexander Webber. "Dissemination of IncF plasmids carrying beta-lactamase genes in Gram-negative bacteria from Nigerian hospitals." Journal of Infection in Developing Countries 7, no. 05 (2013): 382–90. http://dx.doi.org/10.3855/jidc.2613.

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Introduction: Production of beta-lactamases is the predominant cause of resistance to beta-lactam antibiotics in Gram-negative bacteria. We investigated the diversity of plasmid-borne beta-lactamase genes and replicon type of the plasmids carrying the respective genes in Gram-negative bacteria recovered from clinical infection in Nigerian hospitals. Methodology: A total of 134 Gram-negative bacteria of 13 species were analyzed for antimicrobial susceptibility, phenotypic and genotypic detection of various beta-lactamases, and plasmid analysis, including replicon typing. Results: Of the 134 iso

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Suleiman, R., B. Muhammad, and A. M. Magashi. "SURVEY ON MULTI-DRUG RESISTANT AND EXTENDED SPECTRUM BETA LACTAMASES PRODUCING BACTERIA ON CONTACT SURFACES AT MURTALA MUHAMMAD SPECIALIST HOSPITAL, MAKKAH EYE CLINIC AND UMC ZHAHIR HOSPITAL, KANO STATE." FUDMA JOURNAL OF SCIENCES 7, no. 6 (2023): 221–26. http://dx.doi.org/10.33003/fjs-2023-0706-2119.

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The contaminated environmental surfaces are not only potential reservoirs for spread of microbial agents inside hospital but also in community. The development and evolution of antimicrobial resistance in pathogen has been reported to be one of the major issues confronting the global health community. Members of family Enterobacteriacae able to produce extended spectrum of beta lactamase which is responsible to hydrolyze the third generation of cephalosporin group antibiotics resulting treatment failure. The use of beta-lactams has increased considerably since its discovery so also resistant g

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Rahma, Trabelsi, Yengui Mariem, Mhaya Amel, Rebai Ahmed, Arpin Corinne, and Gdoura Radhouane. "Detection of extended-spectrum beta-lactamase and scarbapenemase-producing Enterobacteriaceae in Tunisia." Archives of Biotechnology and Biomedicine 7, no. 1 (2023): 001–11. http://dx.doi.org/10.29328/journal.abb.1001034.

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The emergence of dramatic urinary tract infections (UTIs) caused by the members of the Enterobacteriales is an important public health problem in the community as well as in Tunisian hospitals. This study aims to investigate the prevalence of extended-spectrum β-lactamase (ESBL) and carbapenemase-producing uropathogenic isolates of Escherichia coli (E. coli) and Klebsiella pneumoniae (K. pneumoniae). Based on decreased susceptibility to β-lactams antibiotics and analyzed for the presence of ESBL and carbapenemase genes by Real Time- polymerase chain reaction (RT-PCR), 56 uropathogenic isolates

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Manar Abdallah, Soliman Haroun, Amin Tahoun, Yamen Mohammed Hegazy, and Ramadan Eldomany. "Coexistence of blaOXA-48, blaVIM, and blaSHV genes in Klebsiella pneumoniae and Escherichia coli isolated from urinary tract infections: Microbiological and epidemiological analysis." Journal of the Pakistan Medical Association 73, no. 4 (2023): S294—S304. http://dx.doi.org/10.47391/jpma.egy-s4-58.

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Objective: To investigate antimicrobial resistance mechanisms of isolated bacterial strains, and their correlation with virulence profile.Method: The cross-sectional study was conducted in January 2020 at outpatient health centres in Kafrelsheikh Governorate of Egypt, and comprised urine samples from patients regardless of age and gender. Midstream samples were collected into sterile swaps which were kept in ice-cooled boxes until transported to the laboratory within 5h. Antimicrobial resistance profile of the isolated Enterobacteriaceae was done using Kirby–Bauer disk diffusion method and was

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Ishola, A. D., L. T. Ishola та R. T. Lawal. "PCR Detection of Extended Spectrum β-Lactamase from Some Gram Negative Bacteria of Clinical Source". Dutse Journal of Pure and Applied Sciences 10, № 1b (2024): 203–12. http://dx.doi.org/10.4314/dujopas.v10i1b.21.

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Beta-lactamase-producing microorganisms present distinct challenges in health care systems around the world as a result of the extensive utilization of broad-spectrum antibiotics. The objective of this research endeavor was to ascertain the antibiotic resistance profile and presence of β-lactamase genes in Gram-negative bacteria that had been previously identified and collected from laboratory work benches at the Department of Microbiology and Parasitology, UCH, located in Ibadan, South-West Nigeria. The Kirby-Bauer disc diffusion method was used to assess antibiotic susceptibility on all the

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Nwosu, Uchenna Ogbu, Francis Amadi Ibiam, Christiana Onyemaechi Amadi-Ibiam, et al. "Fecal carriage of extended spectrum beta-lactamase and fluoroquinolone resistant gene in non-typhoidal Salmonella enterica isolates from food-producing animals and humans." Journal of Drug Delivery and Therapeutics 13, no. 9 (2023): 128–38. http://dx.doi.org/10.22270/jddt.v13i9.5964.

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This study seeks to determine the feacal carriage of extended spectrum beta-lactamase and fluoroquinolone resistant non-typhoidal Salmonella enterica isolates from food-producing animals and humans. A total of three hundred (300) fecal samples were collected using sterile universal containers from food-producing animals namely (Chicken [100], Pig [100] and humans (100) from Onicha Local Government Area of Ebonyi State and analyzed for the presence of non-typhoidal Salmonella enterica using standard microbiological techniques. Phenotypic detection of extended-spectrum beta-lactamase (ESBL) were

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Kiemde, Dramane, Inês Ribeiro, Soufiane Sanou, et al. "Molecular characterization of beta-lactamase genes produced by community-acquired uropathogenic Escherichia coli in Nouna." Journal of Infection in Developing Countries 14, no. 11 (2020): 1274–80. http://dx.doi.org/10.3855/jidc.11737.

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Introduction: Extended-Spectrum Beta-Lactamases (ESBL) are a common mechanism of bacterial resistance in Enterobacteriaceae. The purpose of this study is to characterize the ESBL genes produced by community-acquired uropathogenic Escherichia coli strains in the Nouna District, in the West-African country, Burkina Faso. Methodology: Samples were collected from non-hospitalized patients who came for consultation at the CMA (Centre Médical avec Antenne chirurgicale) in Nouna and were sent to the laboratory for a urine culture test. The detection of ESBL production by the bacteria was carrie

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Challapa-Mamani, Mabel R., José Yareta, Alexander Fajardo-Loyola, et al. "Acinetobacter baumannii Co-Resistant to Extended Spectrum Beta-Lactamases and Carbapenemases in Six Peruvian Hospital Centers." Microbiology Research 15, no. 4 (2024): 2650–60. https://doi.org/10.3390/microbiolres15040175.

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Objectives: The aim of this study was to describe the phenotypic and molecular characteristics of Acinetobacter baumannii isolates carrying resistance genes to beta-lactams and carbapenems in six Peruvian public hospital centers. Materials and methods: The susceptibility of bacterial isolates was determined using the automated MicroScan system, with interpretation according to the M100 S30 CLSI 2020. Resistance genes were identified by conventional polymerase chain reaction (PCR), and PCR products were visualized by 1% agarose gel electrophoresis. Results: Nine strains (TRU1, PM1, PM2, CUS1, C

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Islam, Sheikh Shahidul, Md Abdul Malek, AKM Fazlul Haque, Kaisar Ali Talukder, and Marufa Zerin Akhter. "Beta Lactamase Genes of Extended Spectrum Beta Lactamase Producing Escherichia coli from Anorectal Sepsis Cases in Bangladesh." Bangladesh Journal of Microbiology 30, no. 1-2 (2016): 23–29. http://dx.doi.org/10.3329/bjm.v30i1-2.28449.

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A microbiological study was carried out to determine the prevalence of extended spectrum beta lactamase (ESBL) producing E. coli in anorectal sepsis patients in Bangladesh. One hundred specimens of pus, swab, or exudates from anorectal sepsis cases were studied. All the 61 isolates of E. coli were found to be highly resistant to most of the drugs used. Among these, 14 multidrug resistant E. coli were examined for ESBL production by double disc diffusion method. Six of these were found to be ESBL positive. PCR analysis revealed that 3 of the 6 isolates had coexistence of blaSHV, blaOXA and blaC

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Pintor-Cora, Alberto, Laura Álvaro-Llorente, Andrés Otero, Jose M. Rodríguez-Calleja, and Jesús A. Santos. "Extended-Spectrum Beta-Lactamase-Producing Enterobacteriaceae in Fresh Produce." Foods 10, no. 11 (2021): 2609. http://dx.doi.org/10.3390/foods10112609.

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Fresh vegetables are an essential part of a healthy diet, but microbial contamination of fruits and vegetables is a serious concern to human health, not only for the presence of foodborne pathogens but because they can be a vehicle for the transmission of antibiotic-resistant bacteria. This work aimed to investigate the importance of fresh produce in the transmission of extended-spectrum β-lactamases (ESBL)-producing Enterobacteriaceae. A total of 174 samples of vegetables (117) and farm environment (57) were analysed to determine enterobacterial contamination and presence of ESBL-producing En

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Nedjai, Sabrina, Abouddihaj Barguigua, Nassima Djahmi, et al. "Prevalence and characterization of extended spectrum beta-lactamase-producing Enterobacter cloacae strains in Algeria." Journal of Infection in Developing Countries 7, no. 11 (2013): 804–11. http://dx.doi.org/10.3855/jidc.3127.

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Introduction: Expended spectrum β-lactamase (ESBL)-producing Enterobacter cloacae is an important nosocomial pathogen. In this study, the prevalence and the molecular epidemiology of ESBL producing E. cloacae strains isolated from various hospitals in Annaba, Algeria were investigated. Methodology: The study involved 63 isolates of E. cloacae obtained during 2009 at the four hospitals in Annaba. The detection of ESBL was performed using the double-disk synergy test and the combined disk test. Minimum inhibitory concentrations (MICs) were determined using the agar dilution method. The presence

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Makavchik, S. A., and N. V. Polischuk. "Distribution of Escherichia coli synthesizing extended spectrum beta-lactamases in livestock farms." Issues of Legal Regulation in Veterinary Medicine, no. 2 (July 7, 2022): 44–48. http://dx.doi.org/10.52419/issn2782-6252.2022.2.44.

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Microorganisms resistant to antimicrobials pose a worldwide threat to humans and animals and are of interest to bacteriologists. Antibiotic resistance of E. coli is being studied all over the world as one of the most resistant and, as a result, dangerous types of microorganisms. Due to the enzyme complex (extended spectrum beta-lactamase) produced by the bacterial cell, E. coli hydrolyzes beta-lactam antibiotics, including third and fourth generation cephalosporins, which makes research in this area extremely relevant.The aim of our study is to analyze the literature data on the distribution o

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GÖRGEÇ, Sündüz, Çiğdem KUZUCU, Barış OTLU, Funda YETKİN, and Yasemin ERSOY. "Investigation of Beta-Lactamase Genes and Clonal Relationship Among the Extended-Spectrum Beta-Lactamase Producing Nosocomial Escherichia coli Isolates." Mikrobiyoloji Bulteni 49, no. 1 (2015): 15–25. http://dx.doi.org/10.5578/mb.8437.

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Adesina, Tomilola, Obinna Nwinyi, Nandita De, Olayemi Akinnola, and Emmanuel Omonigbehin. "First Detection of Carbapenem-Resistant Escherichia fergusonii Strains Harbouring Beta-Lactamase Genes from Clinical Samples." Pathogens 8, no. 4 (2019): 164. http://dx.doi.org/10.3390/pathogens8040164.

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Recently discovered extraintestinal Escherichia fergusonii obtained from non-clinical samples has exhibited the potential for acquiring multiple beta-lactamase genes, just like many extraintestinal Escherichia coli strains. Albeit, they are often omitted or classified as E. coli. This study aimed to, therefore, identify carbapenem-resistant extended-spectrum beta-lactamase (ESBL) producing E. fergusonii isolates from clinical samples, determine their evolutionary relatedness using 16S rRNA sequencing analysis and screen for beta-lactamase genes. A total of 135 septic wound samples were obtaine

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Pestariati, Dr, and Dr Suhariyadi. "Detection of CTX-M Gene in Escherichia coli Producing Extended Spectrum Beta Lactamase (ESBL) Isolated from Patients with Urinary Tract Infection." International Journal of Medical Science and Clinical Invention 8, no. 09 (2021): 5610–14. http://dx.doi.org/10.18535/ijmsci/v8i09.05.

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Escherichia coli is a Gram-negative bacterium from Enterobacteriaceae family causes urinary tract infections (UTI). A major problem encountered in antibiotics therapy is Multiple Drug Resistant Organisms (MDROs). MDROs occur because of the presence of resistance coding genes such as CTX-M which causes bacteria to produce the Extended Spectrum Beta-Lactamase (ESBL) enzyme. This study aims to detect the presence of the CTX-M gene in Extended-Spectrum Beta Lactamase (ESBL) Escherichia coli isolated from UTI patients. The study was conducted at the Institute of Tropical Disease (ITD) Airlangga Uni

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Rezai, Mohammad Sadegh, Ebrahim Salehifar, Alireza Rafiei, et al. "Characterization of Multidrug Resistant Extended-Spectrum Beta-Lactamase-ProducingEscherichia coliamong Uropathogens of Pediatrics in North of Iran." BioMed Research International 2015 (2015): 1–7. http://dx.doi.org/10.1155/2015/309478.

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Escherichia coliremains as one of the most important bacteria causing infections in pediatrics and producing extended-spectrum beta-lactamases (ESBLs) making them resistant to beta-lactam antibiotics. In this study we aimed to genotype ESBL-producingE. coliisolates from pediatric patients for ESBL genes and determine their association with antimicrobial resistance. One hundred of theE. coliisolates were initially considered ESBL producing based on their MIC results. These isolates were then tested by polymerase chain reaction (PCR) for the presence or absence ofCTX,TEM,SHV,GES, andVEBbeta-lact

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Chanal, C., D. Sirot, H. Malaure, M. C. Poupart, and J. Sirot. "Sequences of CAZ-3 and CTX-2 extended-spectrum beta-lactamase genes." Antimicrobial Agents and Chemotherapy 38, no. 10 (1994): 2452–53. http://dx.doi.org/10.1128/aac.38.10.2452.

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Telling, Kaidi, Age Brauer, Mailis Laht, et al. "Characteristics of Extended-Spectrum Beta-Lactamase-Producing Enterobacteriaceae and Contact to Animals in Estonia." Microorganisms 8, no. 8 (2020): 1130. http://dx.doi.org/10.3390/microorganisms8081130.

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We have attempted to define the prevalence and risk factors of extended-spectrum beta-lactamase-producing Enterobacteriaceae (ESBL-Enterobacteriaceae) carriage, and to characterize antimicrobial susceptibility, beta-lactamase genes, and major types of isolated strains in volunteers, with a specific focus on humans in contact with animals. Samples were collected from 207 volunteers (veterinarians, pig farmers, dog owners, etc.) and cultured on selective agar. Clonal relationships of the isolated ESBL-Enterobacteriaceae were determined by whole genome sequencing and multi-locus sequence typing.

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Bouchakour, Mohammed, Khalid Zerouali, Jean David Perrier Gros Claude, et al. "Plasmid-mediated quinolone resistance in expanded spectrum beta lactamase producing enterobacteriaceae in Morocco." Journal of Infection in Developing Countries 4, no. 12 (2010): 779–803. http://dx.doi.org/10.3855/jidc.796.

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Introduction: Although independently acquired, plasmid-mediated quinolone resistance appears to be linked with extended-spectrum or AmpC-type beta-lactamases. Since no data are available in African countries, the prevalence of qnr genes at the University Hospital Ibn Rochd, Casablanca, Morocco, was investigated. Methodology: Between October 2006 and March 2007, the following 39 randomly selected non-duplicate Enterobacteriaceae producing an extended-spectrum beta-lactamase (ESBL), representing 20% of ESBL strains with respect to species and ward origin, were collected: Escherichia coli (n = 16

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Akinbami, Olajumoke R., Samson Olofinsae, and Funmilola A. Ayeni. "Prevalence of extended spectrum beta lactamase and plasmid mediated quinolone resistant genes in strains of Klebsiella pneumonia, Morganella morganii, Leclercia adecarboxylata and Citrobacter freundii isolated from poultry in South Western Nigeria." PeerJ 6 (June 22, 2018): e5053. http://dx.doi.org/10.7717/peerj.5053.

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A serious concern is arising on the coexistence of extended-spectrum beta-lactamase (ESBL) and plasmid mediated quinolone resistance (PMQR) producing bacteria in animal husbandry, which could be transferred to humans, especially in strains that may not be routinely screened for resistance. This study therefore tested the prevalence of ESBL and PMQR genes in selected bacteria isolated from poultry faeces. Faecal droppings of birds were collected from 11 farms in five states in South Western Nigeria. Bacteria were isolated from the samples on cefotaxime supplemented plates and identified with MA

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Hussein, Ayan Ahmed, Kennedy Kassaza, James Mwesigye, et al. "Characterization of Extended Spectrum Beta-lactamase Uropathogens Isolated from Refugees with Urinary Tract Infections in Nakivale Refugee Settlement Camp, Southwestern Uganda." International STD Research & Reviews 13, no. 2 (2024): 67–78. http://dx.doi.org/10.9734/isrr/2024/v13i2181.

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The World Health Organization estimates one in four individuals has had at least one urinary tract infection (UTI) episode requiring treatment with an antimicrobial agent. At Nakivale refugee camp, the overwhelming number of refugees often associated with poor living conditions predispose the refugees to urinary tract infections. This study determined the prevalence of UTIs, the antimicrobial susceptibility pattern of the isolated bacterial pathogens, the prevalence of Extended-Spectrum Beta-Lactamase (ESBL) bacteria, and the molecular characterization of genes encoding ESBLs among refugees in

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Chub, Olga I., Aleksandr V. Bilchenko, and Igor Khalin. "Extended Spectrum Beta-Lactamase Production in Uropathogens Isolated from Hospitalized Patients with Chronic Pyelonephritis." Open Urology & Nephrology Journal 8, no. 1 (2015): 71–75. http://dx.doi.org/10.2174/1874303x01508010071.

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Background : Increased multidrug resistance of extended-spectrum beta-lactamases (ESBLs) compromises the efficacy of treatment of urinary tract infections. Objective : The objective of this study is to determine the prevalence of ESBL-producing uropathogens from hospitalized patients with chronic pyelonephritis and to identify the presence of genes involved in the resistance. Methods : A cross-sectional study of 105 patients with chronic pyelonephritis, treated in Kharkiv City Clinical Emergency Hospital, Ukraine was carried. Bacterial isolates were collected, antimicrobial susceptibility of i

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Hassan, Manal I., Khaled R. Alkharsah, Alhusain J. Alzahrani, Obeid E. Obeid, Amar H. Khamis, and Asim Diab. "Detection of extended spectrum beta-lactamases-producing isolates and effect of AmpC overlapping." Journal of Infection in Developing Countries 7, no. 08 (2013): 618–29. http://dx.doi.org/10.3855/jidc.2919.

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Introduction: Few reports about the prevalence and genetic basis of extended spectrum beta-lactamases (ESBLs) are available from Saudi Arabia. We sought to determine the prevalence of ESBL-producing Enterobacteriaceae in a university hospital in eastern Saudi Arabia and to characterize the ESBLs produced by these isolates at the molecular level. Methodology: All clinical isolates of Escherichia coli, Klebsiella spp., and Proteus spp. collected over two years were evaluated for susceptibility to a panel of antimicrobials and were analyzed for the ESBL phenotype using screening and confirmatory

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Baguy, Ouattara Mohamed, Affou Séraphin Wognin, Toty Abalé Anatole, et al. "Role of Healthy Human Gut Microbiota in the Emergence and Dissemination of Extended-Spectrum \(\beta\)-lactamase-Producing Enterobacteriaceae and Genes Associated with \(\beta\)-lactam Resistance in Community Settings in Abidjan, Côte d'Ivoire." Microbiology Research Journal International 33, no. 7 (2023): 27–37. http://dx.doi.org/10.9734/mrji/2023/v33i71393.

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Overuse of β-lactam antibiotics in communities in developing countries has transformed healthy human intestinal flora into a reservoir of antibiotic-resistant organisms. The prevalence of extended-spectrum β-lactamase (ESBL)-producing Enterobacteriaceae in community settings remains undetermined. In order to obtain data on ESBL enterobacteria, 265 stool samples were collected from August 2019 to February 2020 from individuals residing in the urban districts of Abidjan and attending medical consultations at the Institut Pasteur de Côte d'Ivoire. Isolates belonging to family Enterobacteriaceae w

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Skwor, Troy, Dan Christopher Jones, Caitlin Cahak, and Ryan J. Newton. "First Report and Characterization of a Plasmid-Encoded blaSFO-1 in a Multi-Drug-Resistant Aeromonas hydrophila Clinical Isolate." Microorganisms 12, no. 3 (2024): 494. http://dx.doi.org/10.3390/microorganisms12030494.

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Antibiotic resistance remains one of the most pressing public health issues facing the world today. At the forefront of this battle lies the ever-increasing identification of extended-spectrum beta-lactamases and carbapenemases within human pathogens, conferring resistance towards broad-spectrum and last-resort antimicrobials. This study was prompted due to the identification of a pathogenic Aeromonas hydrophila isolate (strain MAH-4) collected from abdominal fluid, which presented a robust resistance pattern against second-, third-, and fourth-generation cephalosporins, ertapenem, ciprofloxac

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Sękowska, Alicja, and Tomasz Bogiel. "The Evaluation of Eazyplex® SuperBug CRE Assay Usefulness for the Detection of ESBLs and Carbapenemases Genes Directly from Urine Samples and Positive Blood Cultures." Antibiotics 11, no. 2 (2022): 138. http://dx.doi.org/10.3390/antibiotics11020138.

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Increasing antimicrobial resistance of Gram-negative rods is an important diagnostic, clinical and epidemiological problem of modern medicine. Therefore, it is important to detect multi-drug resistant strains as early on as possible. This study aimed to evaluate Eazyplex® SuperBug CRE assay usefulness for beta-lactamase gene detection among Gram-negative rods, directly from urine samples and positive blood cultures. The Eazyplex® SuperBug CRE assay is based on a loop-mediated isothermal amplification of genetic material and allows for the detection of a selection of genes encoding carbapenemas

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COZMA, Andreea Paula, Iulia Elena MACIUCA, Cristina Mihaela RIMBU, Serban MOROSAN, Lucia Carmen TRINCA, and Dorina TIMOFTE. "PREVALENCE AND CHARACTERISATION OF EXTENDED-SPECTRUM BETA-LACTAMASES AND PLASMID-MEDIATED QUINOLONES RESISTANCE IN Enterobacteriaceae ISOLATED FROM COMPANION ANIMALS." Journal of Applied Life Sciences and Environment 56, no. 4(196)/2023 (2024): 541–49. http://dx.doi.org/10.46909/alse-564115.

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Antimicrobial resistance is a major public health concern worldwide. This study aims to determine the prevalence of Enterobacterales producing beta-lactamase (TEM, SHV, OXA) or extended-spectrum beta-lactamases (ESBL), as well as plasmid-mediated resistance to quinolones (PMQR) (qnrA, qnrB, qnrS) in companion animals from the northeast region of Romania. A total of 124 faecal samples were collected aseptically from healthy dogs attending the veterinary practice for vaccination and cultivated on Brilliance ESBL medium (Oxoid, UK). The ESBL production testing was performed using the combination

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Hamasaeed, Payman Akram, and Shayma Jabar Hyder. "Silver nanoparticle Bacteriostatic activity Assessment against Extended-Spectrum Beta-Lactamase Producing Uropathogenic Klebsiella pneumonia." Journal of University of Raparin 7, no. 2 (2020): 288–304. http://dx.doi.org/10.26750/vol(7).no(2).paper13.

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The aims: Screen for the Extended-Spectrum Beta-Lactamase producing Klebsiella pneumonia from urinary tract infection UTI patients phenotypically and molecularly, also testing different sizes of silver nanoparticles as antibacterial for the resistance isolates. Methodology: Vitek Extended-Spectrum Beta-Lactamase test , molecular confirmed ESBL-producing isolates were checked by PCR for the presence of AmpC and blaCTX-M genes. Flat well microtiter plates were used for packing of antibacterial activity of silver nanoparticles . This study included 85 urine samples were collected from patients th

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Ghatak, S., A. Singha, A. Sen, et al. "Detection of New Delhi Metallo-beta-Lactamase and Extended-Spectrum beta-Lactamase Genes inEscherichia coliIsolated from Mastitic Milk Samples." Transboundary and Emerging Diseases 60, no. 5 (2013): 385–89. http://dx.doi.org/10.1111/tbed.12119.

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Najjuka, Christine F., David Patrick Kateete, Dennis K. Lodiongo, et al. "Prevalence of plasmid-mediated AmpC beta-lactamases in Enterobacteria isolated from urban and rural folks in Uganda." AAS Open Research 3 (November 30, 2020): 62. http://dx.doi.org/10.12688/aasopenres.13165.1.

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Background: AmpC beta-lactamase-producing bacteria are associated with increased resistance to third-generation cephalosporins. Here, we describe plasmid-mediated AmpC beta-lactamase-producing enterobacteria isolated from urban and rural dwellers in Uganda. Methods: Stool and urine from 1,448 individuals attending outpatient clinics in Kampala and two rural districts in central Uganda were processed for isolation of Escherichia coli and Klebsiella. Following antibiotic susceptibility testing, cefoxitin resistant isolates, and amoxicillin/clavulanate resistant but cefoxitin susceptible isolates

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Adibe-Nwafor, Jennifer Onyekachi, Ikemesit Udeme Peter, Kenneth Adibe Nwafor, et al. "Pisciculture and Fish Parts as Non-clinical Source of CTX-M and TEM Extended Spectrum Beta-lactamases Producing Escherichia coli in Southeastern Nigeria." Asian Journal of Fisheries and Aquatic Research 23, no. 5 (2023): 18–25. http://dx.doi.org/10.9734/ajfar/2023/v23i5613.

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Background and Objectives: Antimicrobial agent use in pisciculture exerts a selective pressure, resulting in a reservoirs of extended spectrum beta-lactamase producing Escherichia coli in the aquatic environment. Here we screened pisciculture and fish parts as non-clinical source of CTX-M and TEM Extended Spectrum Beta-lactamases (ESBL) producing Escherichia coli in Southeastern Nigeria Methodology: A total of sixty fish parts [fish gills, body part and intestinal swab samples] were collected from pisciculture farm A, C, F and G within Abakaliki metropolis. The samples were bacteriologically a

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Amoudizaj, Fatemeh F., Elnaz Aghayi, Milad G. Matin, Nayemeh Soltani, and Pejman Mala. "Antibiotic Resistance Pattern and Frequency of PER-1, SHV-1 and AMPC Type B-Lactamase Genes in Pseudomonas aeruginosa Isolated from Clinical Samples." Open Microbiology Journal 13, no. 1 (2019): 308–12. http://dx.doi.org/10.2174/1874285801913010308.

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Background: The existence of Extended Spectrum B-lactamase (ESBL) genes plays an important role in spreading B-lactam antibiotic resistance in the producing strains of these enzymes. The resistance of gram-negative bacteria, such as Pseudomonas aeruginosa, to different antimicrobial agents, especially B-lactams, has increasingly been reported. Objective: This study was conducted to determine the prevalence of TEM-1and VEB-1 beta-lactamases gene in P. aeruginosa isolates through Polymerase Chain Reaction (PCR) method. Methods: 100 clinical isolates of P. aeruginosa were collected from different

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