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Rozprawy doktorskie na temat „Fast protein liquid chromatography”

Autor: Grafiati

Data publikacji: 4 czerwca 2021

Data aktualizacji: 1 lutego 2022

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1

Jones, Karen Lorraine. "Analysis of ferredoxin and flavodoxin in Anabaena and Trichodesmium using fast protein liquid chromatography." PDXScholar, 1988. https://pdxscholar.library.pdx.edu/open_access_etds/3812.

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Iron is an essential nutrient for growth of photosynthetic microorganisms such as cyanobacteria and algae. Iron is required for proteins involved in the important processes of carbon and nitrogen assimilation. Low concentrations of iron in cultures or natural waters can lead to iron limitation which affects many aspects of algal metabolism. In natural waters, iron limitation can have effects on the patterns and rates of primary productivity. The cellular content of certain proteins can be affected by media iron concentrations. Methods have been used that assay components of the cell as an indi

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Nguyen, Nhung Phuong. "Axial Ligand Mutant: H229A." Digital Archive @ GSU, 2008. http://digitalarchive.gsu.edu/honors_theses/1.

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Many pathogenic bacteria use their iron acquisition mechanisms to live inside hosts. Streptococcus pyogenes is a pathogenic bacterium that uses streptococcal iron acquisition ABC transporter to obtain heme. SiaA (HtsA, spy1795), a lipoprotein located on the cell surface, serves as a heme binding protein. To understand the iron-uptake mechanism, histidine 229, one of the two proposed axial ligands in SiaA, was mutated to alanine. SiaA H229A was expressed in E. coli, lysed by French Press, and purified by fast protein liquid chromatography (FPLC). SDS-PAGE indicated that pure protein was isolat

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3

Kirk, John Daniel. "Particle beam LC/MS with fast atom bombardment." Thesis, Georgia Institute of Technology, 1990. http://hdl.handle.net/1853/27127.

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Edwardson, P. A. D. "High Performance Liquid Chromatography of polynucleotides and proteins." Thesis, London School of Hygiene and Tropical Medicine (University of London), 1987. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.376534.

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Smid, Jerusa. "Poliformismos do gene da proteína príon celular em pacientes com doença de Alzheimer." Universidade de São Paulo, 2011. http://www.teses.usp.br/teses/disponiveis/5/5138/tde-24052011-142607/.

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INTRODUÇÃO: Os polimorfismos do gene da proteína priônica (PRNP) podem estar associados a doenças neurológicas não priônicas. Estudos em pacientes com doença de Alzheimer (DA) apontam para possível associação entre os polimorfismos do códon 129 do PRNP e DA. Essa associação não foi estudada na população brasileira. Neste estudo, descrevemos a associação entre os diferentes polimorfismos do PRNP e DA. MÉTODOS: Foi estudada amostra composta por 100 pacientes com DA, acompanhados no Ambulatório de Neurologia Cognitiva e do Comportamento e no Centro de Referência em Distúrbios Cognitivos do Hospit

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Bian, Juan. "Liquid Chromatography and Mass Spectrometry Based Analytical Method Development Towards Fast and Sensitive Analysis." The Ohio State University, 2019. http://rave.ohiolink.edu/etdc/view?acc_num=osu1557242729134942.

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Ansong, Godfred. "Analysis of plant polyphenols by high performance liquid chromatography/mass spectrometry and protein binding." Oxford, Ohio : Miami University, 2004. http://rave.ohiolink.edu/etdc/view?acc%5Fnum=miami1083081905.

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Zhou, Feng. "Protein characterization by capillary isoelectric focusing electrophoresis, reversed phase liquid chromatography and mass spectrometry." Access to citation, abstract and download form provided by ProQuest Information and Learning Company; downloadable PDF file, 103 p, 2008. http://proquest.umi.com/pqdweb?did=1456289241&sid=4&Fmt=2&clientId=8331&RQT=309&VName=PQD.

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9

Muir, Matthew Stewart. "Proteomics of the ovine cataract." Diss., Lincoln University, 2008. http://hdl.handle.net/10182/792.

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The lens of the eye needs to be completely transparent in order to allow all light entering the eye to reach the retina. This transparency is maintained by the highly ordered structure of the lens proteins the crystallins. Any disruption to the lens proteins can cause an opacity to develop which is known as cataract. During cortical cataract formation there is increased truncation of the lens crystallins. It is believed that overactivation of calcium-dependent cysteine proteases, the calpains, is responsible for the increased proteolysis of the crystallins seen during cataractogenesis. Within

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10

Atkinson, Ian E. "Mass Spectrometric Analysis of Environmental Contaminants, Protein Structure and Expression." Cleveland State University / OhioLINK, 2008. http://rave.ohiolink.edu/etdc/view?acc_num=csu1231174291.

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11

Zhao, Bei. "Comparison of Label and Label-free Quantitative Liquid Chromatography Tandem Mass Spectrometry for Protein Biomarker Discovery." The Ohio State University, 2010. http://rave.ohiolink.edu/etdc/view?acc_num=osu1285089805.

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Razzaque, Musaab Aaqib. "Studies of porphyrin glycoconjugates and amino acid-, peptide- and protein- adducts by liquid chromatography-mass spectrometry." Thesis, University of Leicester, 2001. http://hdl.handle.net/2381/30756.

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The extraction, isolation and characterisation of a group of hitherto unreported protoporphyrin glycoconjugates in the rat Harderian gland using high performance liquid chromatography (HPLC), capillary electrophoresis (CE), on-line HPLC/Electrospray ionisation MS (HPLC/ESI-MS) and tandem MS is described. The major glycoconjugate was identified as protoporphyrin-1-O-acyl -xyloside with a smaller amount of protoporphyrin-1-O-acyl -glucoside also detected. In the Harderian glands studied, 50-70% of the porphyrins present were in the form of protoporphyrin glycoconjugates. This is the first report

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13

Hidayah, Siti Nurul [Verfasser]. "Improvement of liquid chromatography for analysis and purification of proteoforms via rational protein purification parameter screening and sample displacement chromatography / Siti Nurul Hidayah." Hamburg : Staats- und Universitätsbibliothek Hamburg Carl von Ossietzky, 2021. http://d-nb.info/1241743037/34.

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14

Nguyen, Anh Mai. "New approaches to preparation of macroporous monoliths for use in liquid chromatography." Doctoral thesis, Umeå : Department of Chemistry, Umeå Univ, 2009. http://urn.kb.se/resolve?urn=urn:nbn:se:umu:diva-20890.

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15

Holler, Christopher J. "Purification of an acidic recombinant protein from transgenic tobacco." Thesis, Virginia Tech, 2007. http://hdl.handle.net/10919/32379.

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Tobacco has been studied as a host for producing recombinant therapeutic proteins on a large-scale, commercial basis. However, the proteins expressed in tobacco usually need to be purified to high yield and purity from large amounts of biomass in order for their production to be commercially viable. The methods needed to purify proteins from tobacco are very challenging and not well studied. The objective of this research was to develop a process for the purification of the acidic model protein, recombinant Î²-glucuronidase (rGUS), from transgenic tobacco leaf tissue to high yield and purit

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16

Cai, Yi. "Coupling Ambient Ionization Mass Spectrometry with Liquid Chromatography and Electrochemistry and Their Applications." Ohio University / OhioLINK, 2016. http://rave.ohiolink.edu/etdc/view?acc_num=ohiou1472816263.

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Sun, Xiaobo. "Forensic Applications of Gas Chromatography/Mass Spectrometry, High Performance Liquid Chromatography--Mass Spectrometry and Desorption Electrospray Ionization Mass Spectrometry with Chemometric Analysis." Ohio University / OhioLINK, 2012. http://rave.ohiolink.edu/etdc/view?acc_num=ohiou1329517616.

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18

Chang, Chih-Hsiang. "Proteomic studies on protein N-terminus and peptide ion mobility by nano-scale liquid chromatography/tandem mass spectrometry." Doctoral thesis, Kyoto University, 2021. http://hdl.handle.net/2433/263596.

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19

CHILAKALA, SUJATHA. "DEVELOPMENT OF LIQUID CHROMATOGRAPHY-MASS SPECTROMETRIC ASSAYS AND SAMPLE PREPARATION METHODS FOR THE BIOLOGICAL SAMPLE ANALYSIS." Cleveland State University / OhioLINK, 2017. http://rave.ohiolink.edu/etdc/view?acc_num=csu1512927043412916.

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WINTERS, MICHAEL SHAWN. "PROBING PROTEIN-PROTEIN INTERACTIONS in vitro and in vivo WITH CYANOGEN." University of Cincinnati / OhioLINK, 2002. http://rave.ohiolink.edu/etdc/view?acc_num=ucin1027090541.

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21

Nukareddy, Praveena. "Quantification Of Mouse Cardiac Troponin I And Myosin Binding Protein C Phosphorylation By Liquid Chromatography-Mass Spectrometry (lc-Ms)." ScholarWorks @ UVM, 2018. https://scholarworks.uvm.edu/graddis/986.

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Heart failure is a major public health issue, with its prevalence estimated to be 6.5 million adults in the USA. Of the hospitalized heart failure (HF) cases, 50% are characterized by preserved ejection function (HFpEF). In HFpEF, the heart pumps a normal proportion of blood that enters it. However, thickening of the ventricular walls inhibits the chamber filling to normal volume. The direct basis of HFpEF is a slowed elongation of the cardiac muscle during the diastolic phase of the cardiac cycle. Elucidation of mechanisms that mediate relaxation of cardiac muscle could help understand the pa

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22

Miao, Zhixin. "Development and Applications of Liquid Sample Desorption Electrospray Ionization Mass Spectrometry (DESI-MS)." Ohio University / OhioLINK, 2012. http://rave.ohiolink.edu/etdc/view?acc_num=ohiou1347559532.

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Ramström, Margareta. "Analysis of Complex Biological Samples using Liquid Chromatography-Fourier Transform Ion Cyclotron Resonance Mass Spectrometry." Doctoral thesis, Uppsala University, Analytical Chemistry, 2005. http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-5729.

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<p>Studies of protein and peptide expression are vital in order to understand complex biological systems. As demonstrated in this thesis, on-line packed capillary liquid chromatography-Fourier transform ion cyclotron resonance mass spectrometry (LC-FTICR MS) is a useful analytical tool for such studies.</p><p>A proteomics method, based on global tryptic digestion and subsequent separation and detection of the peptides by LC-FTICR MS, was developed for qualitative analysis of body fluids. Initial experiments on cerebrospinal fluid (CSF) provided results that were comparable or superior to those

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24

Wagner, Knut. "Development of a comprehensive on-line multidimensional high performance column liquid chromatography system for protein and peptide mapping with integrated size selective sample fractionation." [S.l.] : [s.n.], 2001. http://ArchiMeD.uni-mainz.de/pub/2001/0143/diss.pdf.

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Paiva, Marcelo Vitor de. "Otimiza??o e valida??o de m?todos anal?ticos para a determina??o simult?nea de tuberculost?ticos (4-FDC) por CLAE/DAD e CLUE/ DAD." Universidade Federal do Rio Grande do Norte, 2013. http://repositorio.ufrn.br:8080/jspui/handle/123456789/13477.

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Made available in DSpace on 2014-12-17T14:16:30Z (GMT). No. of bitstreams: 1 MarceloVPA_DISSERT.pdf: 3152615 bytes, checksum: 2a3513c0df769468fec98423bf2dba29 (MD5) Previous issue date: 2013-03-21<br>Tuberculosis is a serious disease, but curable in practically 100% of new cases, since complied the principles of modern chemotherapy. Isoniazid (ISN), Rifampicin (RIF), Pyrazinamide (PYR) and Chloride Ethambutol (ETA) are considered first line drugs in the treatment of tuberculosis, by combining the highest level of efficiency with acceptable degree of toxicity. Concerning USP 33 - NF28 (

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26

Wieland, Christoph. "Charakterisierung und Modifizierung poröser Cellulosepartikel für die flüssige Hochleistungs-Chromatographie und ihr Einsatz zur Untersuchung von Protein-Wechselwirkungen." Doctoral thesis, Humboldt-Universität zu Berlin, Mathematisch-Naturwissenschaftliche Fakultät I, 2010. http://dx.doi.org/10.18452/16090.

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Perlcellulose stellt ein interessantes Material für den Einsatz in der wässrigen Größenausschlusschromatgraphie (SEC) dar. Sie ist aufgrund ihrer guten Modifizierbarkeit zudem ein perfektes Ausgangsmaterial für Protein-Aggregationsuntersuchungen. Ein Protein von besonderem praktischem Interesse ist Insulin. Dessen Fehlfaltung und Aggregation verursacht eine Reihe von schwerwiegenden Problemen (z.B. in Drug-Delivery-Systemen). Hierbei erfolgt eine Umwandlung von alpha-Helix- in beta-Faltblatt-Strukturen wobei sich unlösliche Fibrillen bilden. Deren Rückfaltung mit Hilfe fluorierter Alkohole sow

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27

Wiberg, Henning. "Analytical Approaches to Neurodegenerative Disease Protein Aggregation." Licentiate thesis, KTH, Analytisk kemi, 2011. http://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-34027.

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Tang, Jianhua. "Development of a Novel Gradient Chromatofocusing Tandem Mass Spectrometry Technique for the Determination of Cationic Compounds in Biofluids; Identification of Caspase 3 Cleavage Sites of NHE-1 by High Performance Liquid Chromatography-Mass Spectrometry." Cleveland State University / OhioLINK, 2009. http://rave.ohiolink.edu/etdc/view?acc_num=csu1247344073.

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Abbaraju, Naga Vijayalaxmi. "Patterns of protein expression in tissues of the killifish, Fundulus heteroclitus and Fundulus grandis." ScholarWorks@UNO, 2011. http://scholarworks.uno.edu/td/113.

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Fundulus is a diverse and widespread genus of small teleost fish of North America. Due to its high tolerance for physiochemical variation (e.g. temperature, oxygen, salinity), Fundulus is a model organism to study physiological and molecular adaptations to environmental stress. The thesis focuses on patterns of protein expression in Fundulus heteroclitus and F. grandis.The patterns of protein expression were investigated using traditional methods of enzyme activity measurements and recent proteomic approaches. The findings of the study can be used to guide future studies on the proteomic respo

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30

Al, Matari Amira. "Development of new analytical methods for the analysis at the intact level of glycoforms of hCG and other gonadotropins by nano liquid chromatography hyphenated to high resolution mass spectrometry." Thesis, Université Paris sciences et lettres, 2021. http://www.theses.fr/2021UPSLS034.

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La glycosylation est l’addition de glycanes sur une protéine, appelée alors glycoprotéine. C'est l'une des modifications post-traductionnelles les plus courantes et elle a un impact sur l'activité et la stabilité de la protéine. L’hormone chorionique gonadotrope humaine (hCG), appelée communément hormone de grossesse, est une protéine hautement glycosylée, composée de deux sous-unités, hCGα et hCGβ, ayant au total quatre sites de N- et quatre sites de O-glycosylation, conduisant à un nombre très élevé de glycoformes. Or, l’état de glycosylation de cette hormone essentielle de la grossesse a ét

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31

Kamble, Sharad R. "Molecular interactions in pharmaceutical preformulation and supramolecular complexes. Structural properties governing drug-plasma protein binding and investigation of amino acids co-crystals." Thesis, University of Bradford, 2018. http://hdl.handle.net/10454/16882.

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The study of pharmaceutical preformulation includes the evaluation of pharmacokinetic, pharmacodynamic and physicochemical properties of the drug molecules that aid the formulation. However, it has a limited role in determining drug dosage optimisation in the formulation. The study of drug-Plasma Protein Binding (PPB), and the lipophilicity, solubility, and ionic behaviours of the desired drug molecules addresses the gap and enhances our undertraining related to the behaviour of the drug molecules in the body. The High-Performance Liquid Chromatography (HPLC) technique was used in the

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32

Jeudy, Jérémy. "Quantification de biomarqueurs protéiques dans des matrices biologiques complexes par spectrométrie de masse : développements et applications." Thesis, Lyon 1, 2014. http://www.theses.fr/2014LYO10242/document.

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Le travail présenté ici s'est penché sur les problèmes rencontrés sur ce type d'études protéomiques, et sur les solutions qui peuvent être explorées pour améliorer le débit d'évaluation des candidats biomarqueurs. Les peptides à méthionine sont généralement évités en raison de leur sensibilité à l'oxydation. Cependant, il semblait intéressant d'étudier leur modification endogène pouvant affecter les processus biologiques. Une première évaluation de l'impact de l'oxydation des apolipoproteins dans le cas de la maladie d'Alzheimer, a permis de mettre de côté ce biomarqueur potentiel, et de faire

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33

Figueiredo, Douglas Borges de. "Desenvolvimento do processo de purificação da proteína A de superfície de pneumococo do clado 4 (PspA4Pro)." Universidade de São Paulo, 2014. http://www.teses.usp.br/teses/disponiveis/87/87131/tde-22102018-152147/.

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A proteína A de superfície de pneumococo (PspA) é encontrada na superfície de todas as cepas de Streptococcus pneumoniae e candidata promissora para novas vacinas pneumocócicas. Foi desenvolvido um processo de purificação da PspA4Pro cujas etapas iniciais foram: ruptura da biomassa celular, precipitação do homogenato obtido com o detergente brometo de cetiltrimetilamônio (CTAB) e remoção do precipitado por centrifugação. Foram avaliadas cromatografias de troca iônica (aniônica, catiônica), afinidade por metais, interação hidrofóbica e mista de troca catiônica e hidrofóbica. Utilizando precipit

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Aguiar, Mike. "Applications of mass spectrometry in clinical chemistry and biomedical research." Thesis, McGill University, 2007. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=108330.

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Note:<br>Clinical chemistry is a medical discipline whose aim is to diagnose and assess disease by analysis of biological specimens. Modem laboratories can perform several hundred different tests using many different methods developed over the last century. The classical, more traditional assays are typically labour-intensive, not multiplexed (only measure one analyte or disorder per assay), expensive, require a long turnaround time, and may not provide adequate sensitivity and specificity. Developments in mass spectrometry (MS) and related technologies over the last two decades have provided

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35

Gorityala, Shashank. "TARGETED AND UNTARGETED OMICS FOR DISEASE BIOMARKERS USING LC-MS." Cleveland State University / OhioLINK, 2018. http://rave.ohiolink.edu/etdc/view?acc_num=csu1547093694357568.

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Collin, Olivier L. "Development of a Novel Tandem Mass Spectrometry Technique for Forensic and Biological Applications." View abstract, 2007. http://gateway.proquest.com/openurl?url_ver=Z39.88-2004&res_dat=xri:pqdiss&rft_val_fmt=info:ofi/fmt:kev:mtx:dissertation&rft_dat=xri:pqdiss:3292877.

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37

Sloupová, Klára. "Izolace čistých aminokyselin z pšeničných otrub." Master's thesis, Vysoké učení technické v Brně. Fakulta chemická, 2021. http://www.nusl.cz/ntk/nusl-449764.

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Wheat bran is a promising material containing a wide range of useful components, including proteins. In addition, it is produced in significant volumes. Currently, wheat bran is used for the production of energy by combustion and for feed purposes. Gradually, new methods of valorization of this material are being sought. One of the possibilities of using wheat bran is the isolation of proteins, hydrolysis, and separation of selected amino acids. This diploma thesis deals with this issue, it is focused on the recovery of arginine and leucine from a protein isolate. Proteins were extracted from

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38

Carvalho, Rimenys Junior. "Produção e purificação de um fragmento recombinante da proteína A de superfície do clado 3 (PspA3) de Streptococcus pneumoniae em Escherichia coli." Universidade de São Paulo, 2009. http://www.teses.usp.br/teses/disponiveis/87/87131/tde-11022010-121523/.

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A proteína A de superfície de pneumococo (PspA) é indispensável para a virulência da bactéria e foi escolhida para a elaboração de uma nova vacina conjugada contra S. pneumoniae. Para tanto foi desenvolvido um processo industrial de produção e purificação do fragmento recombinante da PspA clado 3 em E. coli. Cultivos descontínuos alimentados foram estabelecidos com glicose ou glicerol em reator de 5L, obtendo-se 62g/L de células secas e 3g/L de PspA3. As células foram lisadas por homogeneizador contínuo de alta pressão com eficiência de 96,7%. A centrifugação foi definida como etapa de clarifi

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Bin, Saeedan Abdulaziz S. A. "The role of MMP10 in non-small cell Lung cancer, and pharmacological evaluation of its potential as a target for therapeutic intervention. Investigation of the role of MMP10 in the tumour microenvironment of non-small cell lung cancer using gene, protein and mass spectrometry approaches to determine MMP10’s potential in drug development strategies." Thesis, University of Bradford, 2014. http://hdl.handle.net/10454/14070.

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Non-Small Cell Lung Cancer (NSCLC), which accounts for 80% of all lung cancer cases, is associated with resistance to chemotherapy and poor prognosis. Exploitation of NSCLC-upregulated pathways that can either be targeted by novel therapeutics or used to improve the tumour-delivery of current chemotherapeutics are required. Among the matrix metalloproteinases (MMPs) that are essential for tumour development, MMP10 is a potential candidate as a therapeutic target based on its expression and contribution to NSCLC development. This research aims to explore the expression and functions of MMP10 in

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40

Bin, Saeedan Abdulaziz Saad Abdulaziz. "The role of MMP10 in non-small cell lung cancer, and pharmacological evaluation of its potential as a target for therapeutic intervention : investigation of the role of MMP10 in the tumour microenvironment of non-small cell lung cancer using gene, protein and mass spectrometry approaches to determine MMP10's potential in drug development strategies." Thesis, University of Bradford, 2014. http://hdl.handle.net/10454/14070.

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Simon, Romain. "La quantification ciblée de protéines et peptides par chromatographie liquide couplée à la spectrométrie de masse en tandem : développements analytiques et applications." Phd thesis, Université Claude Bernard - Lyon I, 2012. http://tel.archives-ouvertes.fr/tel-00875965.

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En recherche clinique ou environnementale, les biomarqueurs protéiques présentent un intérêt croissant. Bien que les immuno-dosages restent les méthodes de référence pour leur quantification, les récentes avancées en spectrométrie de masse (MS) font de cette technique une alternative crédible à l'ELISA. Ce travail apporte quelques éléments méthodologiques pour repousser certaines limitations de la MS. D'abord, deux dosages ont été proposés. Celui réalisé chez G. fossarum représente le premier exemple de dosage de la Vitellogénine chez un invertébré par LC-MS/MS. L'un des défis de la méthode pr

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42

SANTANA, PATRICIA M. "Expressão de tireotrofina humana em células de embrião de rim humano (HEK293)." reponame:Repositório Institucional do IPEN, 2016. http://repositorio.ipen.br:8080/xmlui/handle/123456789/26947.

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Submitted by Marco Antonio Oliveira da Silva (maosilva@ipen.br) on 2016-12-22T16:39:39Z No. of bitstreams: 0<br>Made available in DSpace on 2016-12-22T16:39:39Z (GMT). No. of bitstreams: 0<br>Neste trabalho foi transfectada uma linhagem de células embrionárias de rim humano (HEK293) com os genes das subunidades α e β da tireotrofina humana (hTSH), hormônio glicoproteico secretado pela hipófise. Após 5 dias de cultivo obteve-se uma concentração de hTSH no meio condicionado de 0,95μg/mL. O material foi concentrado e purificado utilizando uma estratégia envolvendo duas etapas, uma

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Arnell, Robert. "Development and Validation of Methods for Characterization of Multi-Component Systems in Preparative LC." Doctoral thesis, Uppsala : Acta Universitatis Upsaliensis, 2006. http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-7422.

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Perchepied, Stan. "Nouveaux outils miniaturisés pour l’analyse de biomolécules dans des fluides biologiques." Thesis, Sorbonne université, 2020. https://accesdistant.sorbonne-universite.fr/login?url=http://theses-intra.upmc.fr/modules/resources/download/theses/2020SORUS105.pdf.

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L’analyse de protéines est principalement réalisée selon l’approche bottom-up qui consiste en une digestion enzymatique et l’analyse des peptides obtenus en chromatographie en phase liquide couplée à la spectrométrie de masse en tandem. L’étape de digestion, réalisée en solution, est une procédure longue. L’utilisation de réacteurs à base d’enzymes immobilisées (IMER) ainsi que leur intégration en ligne avec la LC-MS/MS améliore la fiabilité et la sensibilité de la méthode globale. Le premier objectif de cette thèse a été d’évaluer le potentiel des IMER pour la caractérisation de la glycosylat

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Palmblad, Magnus. "Identification and Characterization of Peptides and Proteins using Fourier Transform Ion Cyclotron Resonance Mass Spectrometry." Doctoral thesis, Uppsala universitet, Institutionen för materialvetenskap, 2002. http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-1999.

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Mass spectrometry has in recent years been established as the standard method for protein identification and characterization in proteomics with excellent intrinsic sensitivity and specificity. Fourier transform ion cyclotron resonance is the mass spectrometric technique that provides the highest resolving power and mass accuracy, increasing the amount of information that can be obtained from complex samples. This thesis concerns how useful information on proteins of interest can be extracted from mass spectrometric data on different levels of protein structure and how to obtain this data expe

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Zanolini, Carolina. "Estabilidade de formulações cosméticas antienvelhecimento com hidrolisado de proteína do arroz (Oryza sativa)." Universidade de São Paulo, 2011. http://www.teses.usp.br/teses/disponiveis/9/9139/tde-22122011-083227/.

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O hidrolisado de proteína do arroz (Oryza sativa) apresenta à habilidade de aumentar à produção de fibroblastos e colágeno, devido a esta especificidade, cada vez mais a indústria cosmética mostra interesse em utilizar peptídeos como ativo de suas formulações.O presente trabalho tem como objetivo a avaliação da estabilidade de formulações cosméticas com ou sem a presença das amostras da proteína hidrolisada do arroz por métodos físico-químico e térmico. Ainda como objetivo desenvolvimento de metodologias analíticas para a separação e identificação das amostras de hidrolisada de proteína do arr

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Chen, Rong-Chun, and 陳容峻. "Fast Determination of Phosphorylated Proteins in Biological Samples by Liquid Chromatography- Tandem Mass Spectrometry." Thesis, 2011. http://ndltd.ncl.edu.tw/handle/15225879427163346391.

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碩士<br>高雄醫學大學<br>生物化學研究所<br>99<br>Protein phosphorylation is one of post-translational modifications (PTMs) that served as a central mechanism in signal transduction of biological organisms. Approximately 99% of this modification located on serine and threonine residues, whereas a few occurred on tyrosine residue. However, due to the facility in designing epitopes of antibodies for phosphotyrosine, it facilitated the detection and functional analysis of phosphotyrosine in biological organisms by many methods (such as western blotting, immunoprecipitation, in vitro kinase assay and mass spectrom

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HSIAO, KAI YUN, and 蕭凱云. "Protein Separation on Reversed-Phase Capillary Liquid Chromatography." Thesis, 2000. http://ndltd.ncl.edu.tw/handle/47132463141535814083.

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碩士<br>國立成功大學<br>化學系<br>88<br>In this study, the parameters affecting protein separation on reversed-phase liquid chromatography, such as organic solvent、ion-pairing agent and the pressure were investigated. For the effect of pressure, the thermodynamic principals were applied to infer the cause of observed results. For organic solvent effect, three model proteins, ribonuclease A、lysozyme and α-lactalbumin which have the similar molecular weight and number of amino acids but different hydrophobicity, were judiciously chosen for this investigation. It was found that the separation eff

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(9179615), Yun Yang. "STUDY FOR THE MECHANISM OF PROTEIN SEPARATION IN REVERSED-PHASE LIQUID CHROMATOGRAPHY." Thesis, 2020.

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<p>Liquid chromatography coupling with mass spectrometry (LC/MS) plays an important role in pharmaceutical characterization because of its ability to separate, identify, and quantify individual compounds from the mixture. Polymer brush layer bonded to the silica surface is designed as a novel stationary phase to improve the LC resolution and MS compatibility. The polymer thickness can be controlled to shield the analyte from interacting with the active silanol on the surface and reduce peak tailing. The functional group of the polymer can be changed to tune the selectivity in different separat

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Hou, Chun Tsen, and 侯春岑. "Effect of Pressure on Protein Retention in Reversed-Phase High-Performance Liquid Chromatography." Thesis, 1999. http://ndltd.ncl.edu.tw/handle/45187504867671626285.

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碩士<br>國立成功大學<br>化學系<br>87<br>Pressure-induced change of protein behavior has long been either ignored or observed to occur at high pressures (>1 kbar). In this study, the on-column detection system is used to directly investigated the effect of pressure on protein retention in situ along the column. The range of pressure in this study is from 20 bar to 360 bar. Meanwhile, in order to observe the tertiary structure change of lysozyme under pressure effect, the on-the-fly scan UV spectroscopy (A281nm/292nm) was acquired as well. In this work, three kinds of stationary phases, standard C18, C4 an

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