Gotowe bibliografie tematyczne / Protein and mRNA levels

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Gotowa bibliografia na temat „Protein and mRNA levels”

Autor: Grafiati
Data publikacji: 6 września 2023
Data aktualizacji: 31 lipca 2025

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Artykuły w czasopismach na temat "Protein and mRNA levels"

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Gerstenfeld, L. C., M. H. Finer, and H. Boedtker. "Altered beta-actin gene expression in phorbol myristate acetate-treated chondrocytes and fibroblasts." Molecular and Cellular Biology 5, no. 6 (1985): 1425–33. http://dx.doi.org/10.1128/mcb.5.6.1425-1433.1985.

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Phorbol-12-myristate-13-acetate (PMA), a potent tumor promoter, was shown to have opposite effects on the cellular morphology and steady-state levels of beta-actin mRNA in embryonic chicken muscle fibroblasts and sternal chondrocytes. When fibroblasts were treated with PMA, they formed foci of densely packed cells, ceased to adhere to culture plates, and had significantly reduced levels of beta-actin mRNA and protein. Conversely, when treated with PMA, floating chondrocytes attached to culture dishes, spread out, and began to accumulate high levels of beta-actin mRNA and proteins. In the stern
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Gerstenfeld, L. C., M. H. Finer, and H. Boedtker. "Altered beta-actin gene expression in phorbol myristate acetate-treated chondrocytes and fibroblasts." Molecular and Cellular Biology 5, no. 6 (1985): 1425–33. http://dx.doi.org/10.1128/mcb.5.6.1425.

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Phorbol-12-myristate-13-acetate (PMA), a potent tumor promoter, was shown to have opposite effects on the cellular morphology and steady-state levels of beta-actin mRNA in embryonic chicken muscle fibroblasts and sternal chondrocytes. When fibroblasts were treated with PMA, they formed foci of densely packed cells, ceased to adhere to culture plates, and had significantly reduced levels of beta-actin mRNA and protein. Conversely, when treated with PMA, floating chondrocytes attached to culture dishes, spread out, and began to accumulate high levels of beta-actin mRNA and proteins. In the stern
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Willis, Dianna E., Erna A. van Niekerk, Yukio Sasaki, et al. "Extracellular stimuli specifically regulate localized levels of individual neuronal mRNAs." Journal of Cell Biology 178, no. 6 (2007): 965–80. http://dx.doi.org/10.1083/jcb.200703209.

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Subcellular regulation of protein synthesis requires the correct localization of messenger RNAs (mRNAs) within the cell. In this study, we investigate whether the axonal localization of neuronal mRNAs is regulated by extracellular stimuli. By profiling axonal levels of 50 mRNAs detected in regenerating adult sensory axons, we show that neurotrophins can increase and decrease levels of axonal mRNAs. Neurotrophins (nerve growth factor, brain-derived neurotrophic factor, and neurotrophin-3) regulate axonal mRNA levels and use distinct downstream signals to localize individual mRNAs. However, myel
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Maicas, E., F. G. Pluthero, and J. D. Friesen. "The accumulation of three yeast ribosomal proteins under conditions of excess mRNA is determined primarily by fast protein decay." Molecular and Cellular Biology 8, no. 1 (1988): 169–75. http://dx.doi.org/10.1128/mcb.8.1.169-175.1988.

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The suggestion that compensation for overabundant mRNA of the genes for Saccharomyces cerevisiae ribosomal protein (r-protein) L3, L29, or rp59 occurs by translation repression has been reinvestigated. First, analysis of the distribution of these three mRNAs in polysome profiles revealed no differences between normal and mRNA-overproducing strains, indicating that initiation of r-protein translation is not repressed under conditions of mRNA overaccumulation. Second, experiments involving radioactive pulse-labeling of proteins were done by using a modified method of data collection and analysis
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Maicas, E., F. G. Pluthero, and J. D. Friesen. "The accumulation of three yeast ribosomal proteins under conditions of excess mRNA is determined primarily by fast protein decay." Molecular and Cellular Biology 8, no. 1 (1988): 169–75. http://dx.doi.org/10.1128/mcb.8.1.169.

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The suggestion that compensation for overabundant mRNA of the genes for Saccharomyces cerevisiae ribosomal protein (r-protein) L3, L29, or rp59 occurs by translation repression has been reinvestigated. First, analysis of the distribution of these three mRNAs in polysome profiles revealed no differences between normal and mRNA-overproducing strains, indicating that initiation of r-protein translation is not repressed under conditions of mRNA overaccumulation. Second, experiments involving radioactive pulse-labeling of proteins were done by using a modified method of data collection and analysis
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Veletza, S. V., K. V. Nichols, I. Gross, H. Lu, D. W. Dynia, and J. Floros. "Surfactant protein C: hormonal control of SP-C mRNA levels in vitro." American Journal of Physiology-Lung Cellular and Molecular Physiology 262, no. 6 (1992): L684—L687. http://dx.doi.org/10.1152/ajplung.1992.262.6.l684.

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We have studied hormonal regulation of the surfactant protein C (SP-C) in fetal 18-dah rat lung explants. SP-C mRNA was detected in Northern blots with a specific rat SP-C cDNA probe and quantified by densitometry. Treatment of the explants with dexamethasone resulted in a dose-dependent increase of the SP-C mRNA level. Transcriptional assays have shown that the regulation of SP-C mRNA by dexamethasone involves a transcriptional step. Administration of the cAMP analogues, 8-bromoadenosine 3',5'-cyclic monophosphate (8-BrcAMP) or dibutyryl adenosine 3',5'-cyclic monophosphate (DBcAMP), produced
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Muller, M., J. Gauley, and John J. Heikkila. "Hydrogen peroxide induces heat shock protein and proto-oncogene mRNA accumulation in Xenopus laevis A6 kidney epithelial cells." Canadian Journal of Physiology and Pharmacology 82, no. 7 (2004): 523–29. http://dx.doi.org/10.1139/y04-059.

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In this study, we examined the effect of hydrogen peroxide on the accumulation of various mRNAs encoding heat shock proteins (hsps) and proto-oncogenes in Xenopus A6 kidney epithelial cells. Hydrogen peroxide treatment enhanced the accumulation of hsp90, hsp70, hsp30, c-jun, c-fos, and actin mRNAs with distinct temporal patterns. Although hsp70, c-fos, and c-jun mRNA levels peaked at 1–2 h before declining, hsp30 and hsp90 mRNA levels were maximal at 4–6 h. Other mRNAs, including heat shock cognate hsc70, immunoglobulin binding protein, and ribosomal L8, were unaffected. Treatment of kidney ce
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Laxminarayana, Dama, Islam U. Khan, Nilamadhab Mishra, Irene Olorenshaw, Kjetil Taskén та Gary M. Kammer. "Diminished Levels of Protein Kinase A RIα and RIβ Transcripts and Proteins in Systemic Lupus Erythematosus T Lymphocytes". Journal of Immunology 162, № 9 (1999): 5639–48. http://dx.doi.org/10.4049/jimmunol.162.9.5639.

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Abstract Deficient type I protein kinase A phosphotransferase activity occurs in the T cells of 80% of subjects with systemic lupus erythematosus (SLE). To investigate the mechanism of this deficient isozyme activity, we hypothesized that reduced amounts of type I regulatory (RI) isoform transcripts, RIα and RIβ, may be associated with a diminution of RIα and/or RIβ protein. Sixteen SLE subjects with a mean (±1 SD) SLE disease activity index of 12.4 ± 7.2 were studied. Controls included 16 normal subjects, six subjects with primary Sjögren’s syndrome (SS), and three subjects with SS/SLE overl
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Berger, Lloyd C., Jnanankur Bag, and Bruce H. Sells. "Translation of poly(A)-binding protein mRNA is regulated by growth conditions." Biochemistry and Cell Biology 70, no. 9 (1992): 770–78. http://dx.doi.org/10.1139/o92-117.

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Translational efficiency of a minor group of mRNAs is regulated by serum levels in 3T6 fibroblasts. Included within this group is the poly(A)-binding protein (PABP) mRNA. We analyzed the distribution of PABP mRNA in polysome profiles and found a large percentage of this mRNA to be translationally repressed in both actively growing (~ 60%) and resting cells (~ 70%). Elevated serum levels induced a distinct bimodal distribution of this mRNA between actively translated and repressed fractions. Similarly, treatment of cells with low doses of cycloheximide also generated a partial shift of represse
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Gygi, Steven P., Yvan Rochon, B. Robert Franza, and Ruedi Aebersold. "Correlation between Protein and mRNA Abundance in Yeast." Molecular and Cellular Biology 19, no. 3 (1999): 1720–30. http://dx.doi.org/10.1128/mcb.19.3.1720.

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ABSTRACT We have determined the relationship between mRNA and protein expression levels for selected genes expressed in the yeastSaccharomyces cerevisiae growing at mid-log phase. The proteins contained in total yeast cell lysate were separated by high-resolution two-dimensional (2D) gel electrophoresis. Over 150 protein spots were excised and identified by capillary liquid chromatography-tandem mass spectrometry (LC-MS/MS). Protein spots were quantified by metabolic labeling and scintillation counting. Corresponding mRNA levels were calculated from serial analysis of gene expression (SAGE) fr
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Rozprawy doktorskie na temat "Protein and mRNA levels"

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Simion, Oana-Maria. "Uncoupling proteins mRNA levels in mice lacking acylation-stimulating protein." Thesis, McGill University, 2001. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=33018.

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The etiology of obesity involves imbalanced energy intake and utilization. ASP is an adipose tissue hormone that facilitates adipocyte uptake of serum fatty acids and their storage. Mice lacking ASP have less adipose tissue mass, despite increased food intake, than wild-type littermates. We hypothesize that the unstored fuels are oxidized through UCP (thermogenic mitochondrial carriers).<br>In male ASP-deficient mice mRNA levels were measured by semi-quantitative RT-PCR and the following changes were observed: UCP-1 decreased in all tested tissues, UCP-2 increased by 15% and 6 fold in muscle a
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Byström, Jonas. "Eosinophil Cationic Protein : Expression Levels and Polymorphisms." Doctoral thesis, Uppsala University, Department of Medical Sciences, 2002. http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-2059.

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<p>The eosinophil cationic protein (ECP) is usually associated with the eosinophil granulocyte. In this thesis the presence and production of this protein has been studied in two other cells. The circulating monocyte was found to contain ECP mRNA and small amounts of ECP, one thousand times less than that found in the eosinophil. The production decreased by differentiation of the myelomonoblastic cell line U937 into a macrophage phenotype. Submucosal lung macrophages did not stain for ECP and alveolar macrophages did not contain ECP mRNA. The circulating neutrophil contains ECP at a level hund
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Kachra, Zarin. "Regulation of insulin-like growth factor-I (IGF-I) and IGF-binding protein-1 (IGFBP-1) mRNA levels in cultured rat hepatocytes." Thesis, McGill University, 1993. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=41300.

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The liver is a major site of production of circulating levels of insulin-like growth factor-I (IGF-I) and IGF-binding proteins (IGFBPs). We have used primary cultured rat hepatocytes maintained under serum free conditions to explore the regulatory role of various hormones on hepatic IGF-I and IGFBP-1 mRNA levels.<br>IGF-I mRNA levels were stimulated 2.0 to 2.5 fold by bovine growth hormone (bGH) and 1.8 to 2.0 fold by glucagon but on combining bGH and glucagon, a synergistic effect was observed and IGF-I mRNA level was augmented 10 to 12 fold. Octreotide blocked the hGH induced stimulation of
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Schwanhäußer, Björn. "Global analysis of cellular protein dynamics by pulse-labeling and quanti tati ve mass spectrometry." Doctoral thesis, Humboldt-Universität zu Berlin, Mathematisch-Naturwissenschaftliche Fakultät I, 2011. http://dx.doi.org/10.18452/16305.

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Der erste Teil der Arbeit beschreibt die Etablierung einer modifizierten Form des klassichen SILAC-Verfahrens, das in der quantitativen Massenspektrometrie zur Bestimmung von relativen Änderungen in Proteinmengen benutzt wird. Im sog. „pulsed SILAC (pSILAC)“ Verfahren werden Zellen im Zuge einer differentiellen Behandlung in Kulturmedien transferiert, die unterschiedlich Isotop-markierte Aminosäuren enthalten. Da hier die Quantifizierung auf dem Verhältnis der neusynthetisierten Proteinmengen beruht, können gezielt Unterschiede in der Proteinproduktion bestimmt werden. Mit Hilfe von pSILAC kon
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Van, Rooyen Marina. "The modulating effect of myo-inositol and other antidepressants on the mRNA levels and protein expression of selected subcellular enzymes / Marina van Rooyen." Thesis, North-West University, 2005. http://hdl.handle.net/10394/504.

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myo-lnositol (mIns), a natural component of the human diet and essential precursor of several signalling pathways, including that of G protein-coupled receptors, has also been shown to be effective in the treatment of psychiatric disorders such as depression, obsessive compulsive disorder and panic disorder. Most likely since mlns is a simple isomer of glucose, no serious side effects have been reported with its use, even at high oral doses of mlns. Previous studies suggest that the therapeutic action of mlns may include reduced serotonin 5HTzA and muscarinic acetylcholine receptor function. A
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Peter, Martina Andrea. "Growth hormone-dependent expression and regulation of insulin-like growth factor (IGF) I and IGF binding protein mRNA levels in rat tissues in vivo /." [S.l.] : [s.n.], 1993. http://e-collection.ethbib.ethz.ch/show?type=diss&nr=10291.

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Kogan, Cary. "The expression of neurofilament protein and mRNA levels in the lateral geniculate nucleus and area V1 of the developing and adult vervet monkey (Ceorcopithicus aethiops) /." Thesis, National Library of Canada = Bibliothèque nationale du Canada, 1999. http://www.collectionscanada.ca/obj/s4/f2/dsk1/tape10/PQDD_0028/MQ50807.pdf.

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Beyerle, Jolantha [Verfasser], and Heinz [Akademischer Betreuer] Schmeiser. "Assessment of mRNA, protein levels and activities of xenobiotic metabolizing enzymes in colon and rectal mucosa of colorectal cancer patients / Jolantha Beyerle ; Betreuer: Heinz Schmeiser." Heidelberg : Universitätsbibliothek Heidelberg, 2016. http://d-nb.info/1180736281/34.

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Arslan, Sevki. "Effects Of Benzene On Liver, Kidney And Lung Cyp1a, Cyp2b4, Cyp2e1 And Cyp3a6 Mrna, Protein Level, And Drug Metabolizing Enzyme Activities And Toxicity In Diabetic Rabbits." Phd thesis, METU, 2008. http://etd.lib.metu.edu.tr/upload/3/12609446/index.pdf.

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The effects of diabetes on cytochrome P450 dependent drug metabolizing enzymes have not to be clarified yet. The most widely used animals in these studies have been rats, and information regarding the effects of diabetes on cytochrome P450 dependent procarcinogen/carcinogen metabolism in rabbits is limited. In the present study, we investigated, for the first time, the influence of benzene on liver, kidney and lung microsomal cytochrome P450 dependent drug metabolizing enzyme activities, protein and mRNA levels in diabetic and non-diabetic rabbits. Male New Zealand rabbits were made diabetic
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Beresford, Guy William. "Control of glucokinase and mRNA levels in hepatocytes." Thesis, University of Newcastle Upon Tyne, 1994. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.386292.

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Książki na temat "Protein and mRNA levels"

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Lynne, Maquat, ed. Nonsense-mediated mRNA decay. Landes Bioscience, 2006.

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Berry, Fred Brandon. Calmodulin protein and mRNA expression during postmatal development of the rat brain. National Library of Canada, 1995.

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Kovalchuke, Lyudmila. Regulation of Parkin Protein Levels by L-3,4-dihydroxyphenylalanine. [publisher not identified], 2018.

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J, Kay, Ballard F. J, and Mayer R. J, eds. Gene expression: Regulation at the RNA and protein levels. Biochemical Society, 1989.

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D, Takezawa, and United States. National Aeronautics and Space Administration., eds. Calmodulin gene family in potato: Developmental and touch-induced expression of the mRNA encoding a novel isoform. National Aeronautics and Space Administration, 1997.

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Nargolwalla, Cyra. Modulation of mRNA levels for tPA, PAI-1 & TGF-gbs in rat testicular somatic cells. National Library of Canada = Bibliothèque nationale du Canada, 1991.

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Manzerra, Pasquale. Expression of constitutive hsc70 and stress-inducible hsp70 mRNA and protein in the rabbit central nervous system. National Library of Canada = Bibliothèque nationale du Canada, 1997.

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Stevens, Miata Yvette. Corticotropin-releasing hormone receptor subtype 1 and subtype mRNA expression and protein localization in the myometruim in pregnanct. National Library of Canada, 1998.

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Myllynen, Jenni Eileen. The effect of nerve-mediated activity on the expression of Muscle Regulatory Factor and Myosin Heavy Chain Protein mRNA transcripts. Laurentian University, Behavioural Neuroscience Program, 1997.

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Owh, Phillip. Myelin basic protein up-regulates CD44 levels in an astrocytoma cell line in vitro. National Library of Canada, 1996.

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Części książek na temat "Protein and mRNA levels"

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Matsumoto, Ayaka, Nariaki Matsuura, and Miki Hieda. "Detection of SUN1 Splicing Variants at the mRNA and Protein Levels in Cancer." In The LINC Complex. Springer New York, 2018. http://dx.doi.org/10.1007/978-1-4939-8691-0_21.

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Arnoldi, Michele, Giulia Zarantonello, Stefano Espinoza, Stefano Gustincich, Francesca Di Leva, and Marta Biagioli. "Design and Delivery of SINEUP: A New Modular Tool to Increase Protein Translation." In Methods in Molecular Biology. Springer US, 2022. http://dx.doi.org/10.1007/978-1-0716-2010-6_4.

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AbstractSINEUP is a new class of long non-coding RNAs (lncRNAs) which contain an inverted Short Interspersed Nuclear Element (SINE) B2 element (invSINEB2) necessary to specifically upregulate target gene translation. Originally identified in the mouseAS-Uchl1 (antisense Ubiquitin carboxyl-terminal esterase L1) locus, natural SINEUP molecules are oriented head to head to their sense protein coding, target gene (Uchl1, in this example). Peculiarly, SINEUP is able to augment, in a specific and controlled way, the expression of the target protein, with no alteration of target mRNA levels. SINEUP i
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Zhou, Haiyan. "Design of Bifunctional Antisense Oligonucleotides for Exon Inclusion." In Methods in Molecular Biology. Springer US, 2022. http://dx.doi.org/10.1007/978-1-0716-2010-6_3.

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AbstractBifunctional antisense oligonucleotide (AON) is a specially designed AON to regulate pre-messenger RNA (pre-mRNA) splicing of a target gene. It is composed of two domains. The antisense domain contains sequences complementary to the target gene. The tail domain includes RNA sequences that recruit RNA binding proteins which may act positively or negatively in pre-mRNA splicing. This approach can be designed as targeted oligonucleotide enhancers of splicing, named TOES, for exon inclusion; or as targeted oligonucleotide silencers of splicing, named TOSS, for exon skipping. Here, we provi
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Smeland, Erlend B., Tore Godal, Klaus Beiske, Rosemary Watt, Susan Pfeifer-Ohlsson, and Rolf Ohlsson. "Regulation of c-myc mRNA and Protein Levels During Activation of Normal Human B Cells." In Current Topics in Microbiology and Immunology. Springer Berlin Heidelberg, 1986. http://dx.doi.org/10.1007/978-3-642-71562-4_43.

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Bradbury, Joshua J., Holly E. Lovegrove, Marta Giralt-Pujol, and Shane P. Herbert. "Analysis of mRNA Subcellular Distribution in Collective Cell Migration." In Cell Migration in Three Dimensions. Springer US, 2023. http://dx.doi.org/10.1007/978-1-0716-2887-4_22.

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AbstractThe movement of groups of cells by collective cell migration requires division of labor between group members. Therefore, distinct cell identities, unique cell behaviors, and specific cellular roles are acquired by cells undergoing collective movement. A key driving force behind the acquisition of discrete cell states is the precise control of where, when, and how genes are expressed, both at the subcellular and supracellular level. Unraveling the mechanisms underpinning the spatiotemporal control of gene expression in collective cell migration requires not only suitable experimental m
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Mirlekar, Bhalchandra, Daniel Michaud, and Yuliya Pylayeva-Gupta. "IL-35 Detection in B Cells at the mRNA and Protein Level." In Methods in Molecular Biology. Springer US, 2021. http://dx.doi.org/10.1007/978-1-0716-1237-8_8.

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Fawcett, Tony, William J. Simon, John Shanklin, and Antoni R. Slabas. "Expression of MRNA and Steady-State Levels of Protein Isoforms of Enoyl-ACP Reductase From Brassica napus." In Plant Lipid Metabolism. Springer Netherlands, 1995. http://dx.doi.org/10.1007/978-94-015-8394-7_25.

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Boudreau-Larivière, Céline, Roxanne Y. Y. Chan, and Bernard J. Jasmin. "Activity-Linked Regulation of Acetylcholinesterase mRNA Levels Involves Distinct Molecular Mechanisms in Developing Versus Adult Skeletal Muscles." In Structure and Function of Cholinesterases and Related Proteins. Springer US, 1998. http://dx.doi.org/10.1007/978-1-4899-1540-5_26.

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Lackner, K. J., W. März, G. Wolter, H. Sartor, and W. Gross. "Anabolic Steroids do not Change mRNA Levels and Protein Secretion of Apolipoprotein A-I and B-100 in HepG2 Cells." In Recent Developments in Lipid and Lipoprotein Research. Springer Berlin Heidelberg, 1993. http://dx.doi.org/10.1007/978-3-642-84855-1_18.

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Matsuno, Akira, Yudo Ishii, Mineko Murakami, et al. "Quantum Dot-Based In Situ Hybridization and Immunohistochemistry to Detect mRNA and Protein at Subcellular Levels, Comparison with Studies Using Electron Microscopy." In In Situ Hybridization Methods. Springer New York, 2015. http://dx.doi.org/10.1007/978-1-4939-2303-8_22.

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Streszczenia konferencji na temat "Protein and mRNA levels"

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Maram, Balajee, Gotte Vasavi, V. Vasudha Rani, B. Ramesh, M. Narendra Nadh Reddy, and Sasibhushana Rao Pappu. "Predictive Models of Cholesterol Levels through Protein Expression Patterns." In 2024 International Conference on Intelligent Systems and Advanced Applications (ICISAA). IEEE, 2024. https://doi.org/10.1109/icisaa62385.2024.10829210.

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Ohlsson, M., A. J. W. Hsueh, and T. Ny. "HORMONE REGULATION OF THE FIBRINOLYTIC SYSTEM IN THE OVARY." In XIth International Congress on Thrombosis and Haemostasis. Schattauer GmbH, 1987. http://dx.doi.org/10.1055/s-0038-1644389.

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In the ovary, the release of oocytes from graafian follicles during hormone-induced ovulation has been found to be associated with substantial increases in follicular plasminogen activator (PA) activity. Most of the PA activity comes from the granulosa cells that have been shown to produce tPA, uPA as well as the type-1 PA-inhibitor,(PAI-1).We have studied the molecular mechanism of follicle stimulating hormone (FSH) and gonadotropin releasing hormone (GnRH) on the synthesis of tPA in primary cultures of rat granulosa cells. FSH and GnRH were both found to induce tPA in granulosa cells in a ti
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Msdcalf, R. L., E. K. O. Kruithof, and W.-D. Schleuning. "TRANSIENT INDUCTION OF PLASMINOGEN ACTIVATOR INHIBITOR 2 (PAI-2) GENE TRANSCRIPTION BY THE TUMOUR PROMOTING PHORBOL ESTER PMA IN THE HUMAN MACROPHAGE-LIKE CELT, LINE U-937." In XIth International Congress on Thrombosis and Haemostasis. Schattauer GmbH, 1987. http://dx.doi.org/10.1055/s-0038-1642857.

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The human hematopoietic cell line U-937 differentiates to a macrophage/monocyte phenotype after exposure to the tumour promoter PMA. We have recently shown that PMA concomitantly induces PAI-2 biosynthesis in these cells. Now, we have employed an 1880 base pair cDNA to study PAI-2 biosynthesis on the level of transcription and mRNA stability. By in vitro elongation of initiated PAI-2 transcripts in isolated nuclei in the presence of 32p-labelled UTP followed by hybridization to cloned DNA (“run-on” transcription assay), we have demonstrated that PAI-2 gene transcription rates are induced 50-fo
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Apostolatos, Christopher, Tracess Smalley та Mildred A. Duncan. "Abstract 3934: Analysis of PKC-ζ protein and mRNA levels in normal and malignant breast tissue". У Proceedings: AACR 107th Annual Meeting 2016; April 16-20, 2016; New Orleans, LA. American Association for Cancer Research, 2016. http://dx.doi.org/10.1158/1538-7445.am2016-3934.

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Schützle, H., M. Weis-Klemm, RE Gay, S. Gay, and WK Aicher. "THU0049 Protein kinase inhibition enhances steady state mrna levels encoding il -16 but reduces il-18 encoding message levels in fibroblasts." In Annual European Congress of Rheumatology, Annals of the rheumatic diseases ARD July 2001. BMJ Publishing Group Ltd and European League Against Rheumatism, 2001. http://dx.doi.org/10.1136/annrheumdis-2001.846.

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Andreasen, P. A., A. Riccio, L. R. Lund, K. G. Welinder, F. Blasi, and K. Danø. "PLASMINOGEN ACTIVATOR INHIBITOR TYPE 1: STUDIES ON STRUCTURE AND REGULATION." In XIth International Congress on Thrombosis and Haemostasis. Schattauer GmbH, 1987. http://dx.doi.org/10.1055/s-0038-1642810.

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Human plasminogen activator inhibitor type-1 is an Mr∼54,000 protein which specifically inhibits urokinase-type (u-PA) and tissue-type (t-PA) plasminogen activators. During inhibition, u-PA and t-PA convert PAI-1 to an inactive form with Mr∼50,000. We have determined the amino-terminal amino acid sequence of native and converted PAI-1, and isolated and partly sequenced PAI-1 cDNA. The data show that the conversion of PAI-1 consists of cleavage of an Arg-Met bond 33 residues from the carboxy-terminus, thus localizing the reactive center of the inhibitor to that position, and identifying PAI-1 a
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Antonacopoulou, A., A. Kottorou, V. Tzelepi та ін. "PO-513 The alternative NF-κB pathway in colorectal cancer. from genetic polymorphisms through mRNA to protein levels". У Abstracts of the 25th Biennial Congress of the European Association for Cancer Research, Amsterdam, The Netherlands, 30 June – 3 July 2018. BMJ Publishing Group Ltd, 2018. http://dx.doi.org/10.1136/esmoopen-2018-eacr25.1014.

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Medcalf, R. L., E. van den Berg, and W.-D. Schleuning. "THE INFLUENCE OF GLUCOCORTICOID HORMONES ON THE GENE TRANSCRIPTION OF POUR COMPONENTS OF THE FIBRINOLYTIC SYSTEM." In XIth International Congress on Thrombosis and Haemostasis. Schattauer GmbH, 1987. http://dx.doi.org/10.1055/s-0038-1644611.

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The hormonal regulation of plasminogen activator (urokinase type (u-PA) and tissue-type (t-PA)) biosynthesis plays an important role in fibrinolysis and extracellular matrix turnover during invasive growth and cell migration. Recently, two genetically distinct inhibitors of both PA's (PA inhibitor 1 (PAI-1) and PA inhibitor 2 (PAI-2)) have been described which may contribute to the modulation of matrix stability. We have employed cloned cDNA probes to study the regulation of biosynthesis of these proteins in the human fibrosarcoma line HT1080. These cells constitutively express high levels of
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You, Jun, Clare E. Yellowley, Henry J. Donahue, and Christopher R. Jacobs. "Physiological Levels of Substrate Deformation Are Less Stimulatory to Bone Cells Compared to Fluid Flow." In ASME 1999 International Mechanical Engineering Congress and Exposition. American Society of Mechanical Engineers, 1999. http://dx.doi.org/10.1115/imece1999-0427.

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Abstract It is believed that bone cells can sense mechanical loading and alter bone external shape and internal structure to efficiently support the load bearing demands placed upon it. However, the mechanism by which bone cells sense and respond to their mechanical environment is still poorly understood. In particular, the load-induced signals to which bone cells respond, e.g. fluid flow, substrate deformation, electrokinetic effects etc., are unclear. Furthermore, there are few studies focused on the effects of physiological strain (strain &amp;lt; 0.5%, Burr, 1996; Owan, 1997) on bone cells
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Gress, C., J. M. Hohlfeld, and M. Müller. "Chip Cytometry for Simultaneous Measurement of Protein and MRNA Markers on Single Cell Level." In American Thoracic Society 2024 International Conference, May 17-22, 2024 - San Diego, CA. American Thoracic Society, 2024. http://dx.doi.org/10.1164/ajrccm-conference.2024.209.1_meetingabstracts.a2553.

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Raporty organizacyjne na temat "Protein and mRNA levels"

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Barash, Itamar, and Robert E. Rhoads. Translational Mechanisms that Govern Milk Protein Levels and Composition. United States Department of Agriculture, 2004. http://dx.doi.org/10.32747/2004.7586474.bard.

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Original objectives: The long term objective of the project is to achieve higher content of protein in the milk of ruminants by modulating the translational machinery in the mammary gland. The first specific aim of the BARD proposal was to characterize responsiveness of various experimental systems to combination of lactogenic hormones and amino acids with particular emphasis on discrimination between the control of total protein synthesis and milk protein synthesis. Based on the results, we planned to proceed by characterizing the stage of protein synthesis in which the stimulation by lactoge
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Barash, Itamar, and Robert Rhoads. Translational Mechanisms Governing Milk Protein Levels and Composition. United States Department of Agriculture, 2006. http://dx.doi.org/10.32747/2006.7696526.bard.

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Original objectives: The long-term goal of the research is to achieve higher protein content in the milk of ruminants by modulating the translational apparatus of the mammary gland genetically, nutritionally, or pharmacologically. The short-term objectives are to obtain a better understanding of 1) the role of amino acids (AA) as regulators of translation in bovine and mouse mammary epithelial cells and 2) the mechanism responsible for the synergistic enhancement of milk-protein mRNA polyadenylation by insulin and prolactin. Background of the topic: In many cell types and tissues, individual A
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Young, Erin, Cem Kuscu, Christine Watkins, and Murat Dogan. Using CRISPR Gene Editing to Prevent Accumulation of Lipids in Hepatocytes. University of Tennessee Health Science Center, 2022. http://dx.doi.org/10.21007/com.lsp.2022.0007.

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CRISPR gene editing is a molecular technology that can be used to silence gene expression. In this experiment, genes that are known to play a role in lipid accumulation in hepatocytes were targeted. Specifically, levels of fatty acid transport proteins 2 and 5 (FATP2 &amp; 5) have been shown to be elevated in cases of non-alcoholic fatty liver disease. The goal of this experiment was to reduce expression of these genes by using a dead Cas9 (dCas9) protein with an attached inhibitory domain (KRAB) that acts on the promotor region. When measuring the mRNA expression, it was determined that the l
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Stern, David, and Gadi Schuster. Manipulation of Gene Expression in the Chloroplast. United States Department of Agriculture, 2000. http://dx.doi.org/10.32747/2000.7575289.bard.

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The steady-state level of a given mRNA is determined by its rates of transcription and degradation. The stabilities of chloroplast mRNAs vary during plant development, in part regulating gene expression. Furthermore, the fitness of the organelle depends on its ability to destroy non-functional transcripts. In addition, there is a resurgent interest by the biotechnology community in chloroplast transformation due to the public concerns over pollen transmission of introduced traits or foreign proteins. Therefore, studies into basic gene expression mechanisms in the chloroplast will open the door
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Meidan, Rina, and Robert Milvae. Regulation of Bovine Corpus Luteum Function. United States Department of Agriculture, 1995. http://dx.doi.org/10.32747/1995.7604935.bard.

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The main goal of this research plan was to elucidate regulatory mechanisms controlling the development, function of the bovine corpus luteum (CL). The CL contains two different sterodigenic cell types and therefore it was necessary to obtain pure cell population. A system was developed in which granulosa and theca interna cells, isolated from a preovulatory follicle, acquired characteristics typical of large (LL) and small (SL) luteal cells, respectively, as judged by several biochemical and morphological criteria. Experiments were conducted to determine the effects of granulosa cells removal
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Christopher, David A., and Avihai Danon. Plant Adaptation to Light Stress: Genetic Regulatory Mechanisms. United States Department of Agriculture, 2004. http://dx.doi.org/10.32747/2004.7586534.bard.

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Original Objectives: 1. Purify and biochemically characterize RB60 orthologs in higher plant chloroplasts; 2. Clone the gene(s) encoding plant RB60 orthologs and determine their structure and expression; 3. Manipulate the expression of RB60; 4. Assay the effects of altered RB60 expression on thylakoid biogenesis and photosynthetic function in plants exposed to different light conditions. In addition, we also examined the gene structure and expression of RB60 orthologs in the non-vascular plant, Physcomitrella patens and cloned the poly(A)-binding protein orthologue (43 kDa RB47-like protein).
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Suriyaphol, Gunnaporn. Study the gene expression of E-cadherin, syndecan1, matrix metalloproteinases-2, -7, -9, -14 and tissue inhibitors of metalloproteinases-1 and -2 in canine oral melanoma. Chulalongkorn University, 2015. https://doi.org/10.58837/chula.res.2015.80.

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The objectives of this study were to 1.) select the suitable reference genes for quantitative real-time polymerse chain reaction in the most common canine oral cancers: oral melanoma (OM) and oral squamous cell carcinoma (OSCC), 2.) study the gene expression of E-cadherin (CDH1), syndecan 1 (SDC1), matrix metalloproteinases-2, -7, -9, -14 (MMP2, MMP7, MMP9, MMP14) and tissue inhibitors of metalloproteinases-1 and -2 (TIMP1, TIMP2) in canine OM at the mRNA level and study the CDH1, SDC1 and Ki-67 protein expression by immunohistochemistry, and 3.) study the association of gene expression and th
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Avihingsanon, Yingyos, Jongkonnee Wongpiyabovorn, and Nattiya Hirankarn. Biomarker discovery in systemic lupus erythematosus: genome-methylation approaches : Research report. Chulalongkorn University, 2010. https://doi.org/10.58837/chula.res.2010.15.

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Discovery of novel biomarkers in lupus nephritis Biomarkers are needed for making diagnosis and prognosis. In lupus nephritis, conventional tests like urinalysis or serum creatinine remain inadequate for patient care. In this proposal, we focused on non-invasive tools like blood and urine mRNAs or proteins. We chose candidate genes involving regulatory T-cell, B-lymphocyte signatures or vascular protective factors. Expression of regulatory cell signature (FOXP3) in peripheral blood mononuclear cells is associated with activity of lupus nephritis. We found FOXP3 mRNA levels in PBMCs from patien
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Abcouwer, Steve P. Demonstration that a mRNA Binding Protein is Responsible for GADD45 mRNA Destabilization. Defense Technical Information Center, 2003. http://dx.doi.org/10.21236/ada418512.

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Woodson, William, Shimon Mayak, and Haim Rabinowitch. Physiological and Molecular Characterization of the Response to Ethylene during Senescence of Carnation Genotypic Variants. United States Department of Agriculture, 1995. http://dx.doi.org/10.32747/1995.7613011.bard.

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The senescence of carnation (Dianthus caryophyllus L.) flowers is associated with increased production of the phytohormone ethylene, which in turn serves to initiate and regulate the processes involved in programmed petal death. We investigated the regulation of ethylene production and petal senescence in carnation. Several carnation genotypes were identified that exhibited extended vase-life in comparison to flowers from typical commercial cultivars. The capacity of these genotypes to produce ethylene during postharvest vase-life and to respond to exogenous ethylene was investigated. Several
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