Dissertationen zum Thema „African wildlife species“

Dissertação de Mestrado Integrado em Medicina Veterinária<br>Due to constant changes in restraint techniques, equipment and even immobilization drugs, wildlife veterinary practices have improved over the past decades. This broad progress coupled with the experience of practitioners contributes towards a safer practice for both the animals and people involved in the procedures. Anesthetic protocols used for chemical immobilizations performed during an internship in South Africa (184 of 245 restrained individuals) were analyzed for the various species approached, taking into account the darting equipment involved, the surrounding environment and some of the main purposes of each intervention. The different chemical immobilizations performed were classified as successful (176) or unsuccessful (8), and the most common complications that affected the procedures were documented. The reasons for the failings that in cases led to a second darting (13 cases) or to the death of animals (3 cases) are also addressed and preventive measures to avoid them were put forward. It is important to analyze the information logged after every immobilization procedure, including personal experiences from each clinical case, and present and disseminate the conclusions, to help wildlife practitioners worldwide avoid future complications.<br>RESUMO - A medicina veterinária associada à vida selvagem têm vindo a evoluir nas últimas décadas graças às constantes mudanças nas técnicas de imobilização, equipamentos e até fármacos utilizados. Estes progressos, aliados à experiência dos veterinários, têm vindo a contribuir para uma prática médica mais segura tanto para os animais como para as equipas envolvidas nos procedimentos. Durante o estágio na África do Sul, os protocolos anestésicos usados nas imobilizações químicas (184 dos 245 indivíduos imobilizados) foram analisados para as diferentes espécies manipuladas, tendo em conta o equipamento de disparo utilizado, o ambiente envolvente e o propósito das intervenções praticadas. As diferentes imobilizações químicas foram classificadas como bem-sucedidas (176) ou não (8), tendo sido referidas as principais complicações que afectaram os procedimentos. Nos casos sem o sucesso anestésico esperado, recorrendo a um segundo dardo (13 casos) ou culminando na morte dos individuos (3 casos), as razões para o insucesso foram discutidas e algumas medidas preventivas para o futuro foram propostas. É importante que este tipo de informação seja sempre analisado após a execução de uma imobilização, divulgando as conclusões dessa análise e respectivas experiências pessoais dos casos, de modo a poderem ser exploradas pelos médicos-veterinários de vida selvagem para evitar complicações futuras.

Growing evidence suggests many widely distributed low-density tropical species are declining, but whether protected areas and the increasingly promoted multi-use community-managed wildlife areas mitigate causal threats is poorly studied. Lack of monitoring data and poorly understood species ecology limit knowledge of patterns, causal mechanisms and degree of abundance or range declines. Working in an African savanna, where bushmeat hunting and cattle-grazing are considered the greatest threats to wildlife, this thesis combined three approaches to study the conservation ecology of widely distributed wildlife at three spatial scales (continent-wide; landscape; home range). The continent-wide conservation status of Kori Bustard Ardeotis kori was assessed by reviewing occurrence records (1863–2009) across its range (14 countries). Range contraction was quantified by examining the proportion of historical records (pre-1970) falling within the area delineated by recent records (1970–2009), finding 8% (southern) and 21% (East Africa) declines in 146 years. In contrast, qualitative evidence from historical published accounts and contemporary expert assessments suggested widespread declines in abundance; thus numbers have declined considerably but without commensurate range contraction. Examination of seasonal home range use using satellite telemetry showed that female Kori are sedentary in central Botswana,with strong site-fidelity to dry-season home ranges associated with resource-rich pan habitats; the species appears sensitive to localised habitat conditions and resource availability. However, an assessment of the differential response of Kori and 20 other large-bodied vertebrates to protected areas, wildlife management areas and unprotected areas showed most species’ abundance was driven by proximity to human settlement,interpreted as sensitivity to unregulated hunting, with no effect of differential cattle stocking densities. Furthermore, the study demonstrates that cattle can affect habitat structure, but their effects on Kalahari wildlife were not perceptible, suggesting that conservationists’ preoccupation in African savannas with cattle and their perceived impacts rather than unregulated hunting may be misplaced.

Sarcoid-like tumours have been reported in Cape mountain zebra (Equus zebra zebra) in two South African game parks recently. These tumours caused severe distress to the animals and also made them unsightly for tourists visiting the parks. The aim of this investigation was to identify and characterize the infectious agent considered to be involved in the aetiology of sarcoid in the Cape mountain zebra. Bovine papillomaviruses (BPV) -1 and -2 deoxyribonucleic acid (DNA) were detected by the polymerase chain reaction (PCR) in sarcoid tumour tissue, but not from blood specimens or unaffected skin. Differentiation between BPV-1 and -2 was made by using the restriction endonuclease BstXI on PCR products of the E5 open reading frame (ORF). A hybridization probe real-time assay was developed for the specific and sensitive detection and differentiation of BPV-1 and -2 DNA in blood, skin and sarcoid tumour samples. For the specific detection of BPV-1, an increase in fluorescence was detected at 640 nm and of BPV-2 at 705 nm. The test is extremely sensitive and able to detect 1.5 genome copies/reaction. The presence of BPV-1 and -2 DNA could be demonstrated in the blood of sarcoid-affected and -unaffected zebras even in the blood of zebras from parks where sarcoids have never been observed. The phylogenetic relationships of the papillomaviruses detected in tumours in the Cape mountain zebra in comparison with a broad selection of papillomavirus sequences available in GenBank were compiled. The papillomavirus sequences retrieved from the zebras were identified as variants of either BPV-1 or BPV-2. The age of the most recent common ancestor for BPV-1 variants is estimated to be 1.40 million years (Mya) and for BPV-2 variants, 0.55 Mya. The age of the most recent common ancestor of BPV-1 and BPV-2 is estimated to be 5.34 Mya. Certain major histocompatibility (MHC) haplotypes are associated with increased risk of sarcoid tumours in horses. The zebras in these parks may have become inbred for the MHC region with increased prevalence for a haplotype, conferring increased risk for sarcoid tumours. Therefore typing system was developed to determine whether or not a high prevalence of sarcoids among zebras is associated with a MHC haplotype. Single strand conformational polymorphism was used to assess the genetic variation in MHC class II genes. The use of DQB and DRB genes demonstrated that genetic variation and sarcoids in the zebras could not be attributed to a specific haplotype. The developed real-time PCR technique was also applied in the detection of cutaneous papillomavirus in two giraffes (Giraffa camelopardalis) which were manifesting cutaneous papillomatosis, in the Kruger National Park and in a fibropapilloma in a sable antelope (Hippotragus niger), on a game farm in the Kimberley district, South Africa. In conclusion, this was the first study to confirm the presence of BPV-1 and -2 DNA in the sarcoid tumours, healthy skin and blood of sarcoid-affected and healthy free-roaming zebras from sarcoidaffected parks. The presence of BPV-1 and -2 DNA in the blood of zebras from parks where sarcoids have not been previously observed was a significant finding.<br>Thesis (PhD)--University of Pretoria, 2010.<br>Veterinary Tropical Diseases<br>unrestricted

Rainforest biodiversity is particularly vulnerable to loss, since the distribution of forests is limited and the vertebrate species that live within these forests have a limited potential to re-colonize deforested areas, especially when their abundance declines to critical levels. Guinea-Bissau (West Africa) is experiencing significant loss of habitats and species diversity; as such, the establishment of an effective conservation programme is urgent in its remaining forested areas. Despite six legislated protected areas, Guinean forests and their wildlife are not safe in reality. This lack of on-the-ground protection is the case for Cantanhez National Park (Tombali region), where this research took place. The park was established in 2007 to protect remnant forests containing unique and endemic Guinean biodiversity, such as the endangered West African chimpanzees (Pan troglodytes verus). Local inhabitant’s attitudes towards protected areas and associated externallydriven conservation programmes are seldom examined in depth in relation to understanding the drivers (livelihood, socio-cultural, and local) of perceptions, which makes conservation problematic. Understanding attitudes to animals, habitats and livelihood risks were the focus of this project, specifically in order to assess perceptions of chimpanzees. Chimpanzees are currently suffering catastrophic declines due to human actions across Africa. Thus a focus on understanding, managing and enhancing people’s perceptions and attitudes towards this species could be vital to its long-term survival. The theoretical approach is based upon (i) examining the construct of sociozoologic scales in this specific socio-cultural context, (ii) elucidating issues in humanwildlife interaction (e.g. conflict such as crop-raiding and positive such as ecotourism potential), (iii) local economies (i.e. level of dependency on forest resources), and (iv) understanding people’s expectations about the future of the National Park as a potential constraint or opportunity for their welfare and livelihoods. Quantitative and qualitative methods were combined to approach these questions. 17 The Guinean sociozoologic scale of Cantanhez clearly divides vertebrate species into (i) “tame”, considered good (e.g. gazelles) and (ii) “hazardous”, considered bad (e.g. hyaenas). Chimpanzees lay exactly in the midpoint. They are considered humans’ close relatives; however, they “misbehave” as astute crop thieves sufficiently to be perceived as a competitor for resources. Since chimpanzees are also seen as very similar to humans, their meat consumption is taboo, which adds the potential for protection. Gender and religion both influence the way locals perceive of and relate to chimpanzees. Women and Muslims tend to be more negative towards this species and the protected area than are men and non-Muslims. Women never exhibited positive attitudes in relation to the protected area, while men appeared to be more engaged with “capitalized” principles, with some awareness about the importance chimpanzees might have in catalyzing the National Park and local economy. This study highlights the need for a management plan to mitigate crop-raiding and the development of sustainable strategies that provide livelihood benefits for both men and women, addressing their distinct needs, outside the protected area.

Thesis (MTech (Nature Conservation))--Cape Peninsula University of Technology, 2016.<br>Largely due to anthropogenic causes, biodiversity and particularly species diversity is changing at an extraordinary rate, with declines in species abundance, community composition and extinction risk being of crucial concern. Monitoring of state variables of biodiversity such as species richness and occupancy are of significant importance in determining the severity of threats placed on species, populations and communities. As a non-invasive monitoring method camera traps are noted as being an effective, accurate and rapid means of compiling species richness estimates of medium to large terrestrial mammals. However, crucial elements of camera trap survey design are rarely empirically addressed, which has questioned the need for a standardised camera trapping protocol. Table Mountain National Park (TMNP) is a protected area that is under serious anthropogenic pressure through urban and peri-urban development. Although it is the last refuge for a number of large mammal species on the Cape Peninsula, current mammalian species richness knowledge within the TMNP are limited. Accurate and current species richness estimates are therefore needed within TMNP and more specifically the Cape of Good Hope (CoGH), which exclusively hosts a number of medium and large mammal species. The aims of this study were to optimise a camera survey protocol for the Peninsula region, with a focus on camera density, placement and survey duration that will enable accurate estimations of medium to large mammal species richness and occupancy.

Mycobaterium bovis is the causative agent of bovine tuberculosis (BTB) and it is a member of the Mycobacterium tuberculosis complex (MTBC). This bacterium has a wide host range of which, cattle is considered as the maintenance host. Humans, goats, wildlife, cats, dogs and lions are also susceptible to the bacterium and are considered putative spillover hosts as infection is not confined in these hosts. Mycobacterium bovis is prevalent in developing countries especially in farmed animals. This presents a problem since BTB is a zoonosis. People living in close contact with infected cattle or those who drink unpasteurized milk are at risk of infection. About 10% of cases of human tuberculosis are thought to be caused by M. bovis. In some instances, wildlife provides a reservoir for the pathogen and transmits it to cattle in farms and poses further risk to humans at the wildlife/livestock/human interface. Certain countries like the United Kingdom where BTB was previously eradicated are experiencing substantial increase in BTB infection. This is thought to be a result of wildlife reservoirs that infect farmed animals, especially cattle. Such reservoirs make eradication of the disease extremely difficult and require programmes to be put in place to control spread of the disease. This makes M. bovis a pathogen of economic importance since the programmes may be costly. In addition, wildlife that is infected cannot be exported and this further affects the economy negatively. In order to control the spread of the pathogen, it is essential to determine the source of infection. However, it is difficult to determine the source or to track the spread of BTB especially in wildlife where animals have unrestricted movement. The inability to conduct epidemiological studies of BTB may be a result of the lack of molecular typing methods that allow bacteria to be identified to strain level rapidly and fairly simpler than culture, thus providing much needed information about the pathogen. In recent years, typing of M. bovis isolates to strain level has been made possible by the development of PCR-based technologies such as IS6110 typing and spoligotyping. These technologies were however, found to be unsuitable for differentiating certain species in the MTBC. Newer technologies based on the variable number of tandem repeats (VNTRs) in organisms have been developed and allow for the differentiation of members in the MTBC, which have a high level of genome homology. These technologies include multiple-locus variable number tandem repeat analysis (MLVA) and mycobacterial interspersed repetitive unit (MIRU)-VNTR analysis. It was also discovered that mycobacteria have genomic regions of difference (RD) that could be used to identify the different species of bacteria in the MTBC. Retrospective studies may play a key role in tracing the source of diseases and following the pattern of transmission. However, in most instances, no fresh samples are available for such studies. For this reason, formalin-fixed paraffin-embedded (FFPE) tissue from wildlife in the Kruger National Park (KNP) was used for conducting a retrospective study aimed at determining the epidemiology of M. bovis in the KNP. However, amplification of DNA derived from FFPE tissue for PCR based techniques has been found to be a difficult exercise and not many standard protocols have been developed and validated for the use of such DNA. In this study, different methods of extraction were used to obtain DNA from FFPE tissue since it is difficult to obtain high quality DNA from such tissue, which is degraded. Formaldehyde, the main component of formalin which is used to fix tissue samples, causes degradation and cross-linking of DNA. In addition, previous studies are inconsistent with regards to the best method to use when extracting DNA from FFPE tissue. Three PCR-based techniques were used to type or identify the isolates in order to standardize a protocol for use in typing isolates from FFPE tissue. These techniques included analysis of the RDs, VNTR based methods i.e. MLVA and MIRU-VNTR and spoligotyping. Since there are many factors that influence the quality of FFPE tissue, samples confirmed BTB positive by VNTR analysis, spoligotyping and IS6110 analysis were used in order to optimize a PCR for FFPE tissue. Furthermore, in order to serve as control samples for spoligotyping and analysis of the RDs, DNA obtained from fresh tissue was also used in the study. Despite the various methods used to extract and to type DNA, the DNA from FFPE tissue provided unspecific results that did not allow for an informative retrospective study of M. bovis. This may be due to the fact that the DNA used had a high degree of degradation from prolonged fixation in formalin. Although M. bovis could not be typed in FFPE tissues, it could be identified by analysis of the regions of difference, more specifically the RD9 region. Amplification of RD9 is thus recommended for use in retrospective studies for diagnostic purposes, especially in cases where highly degraded DNA is used. This region (RD9) should however, only be used as a presumptive diagnosis since RD9 also identifies M. africanum, M. microti, M. pinnipedii, M. caprrae and M. bovis BCG. However, RD9 specifically excludes M. tuberculosis. In the SA context, particularly in the KNP, this allows for some sound inferences since the animals are likely to be infected with M. bovis as opposed to M. tuberculosis. This study highlighted statements in previous studies where it was stated that fixation of tissue in formalin should be done in such a way to reduce degradation of DNA in FFPE tissue in order to allow for its use in retrospective molecular studies which may be very insightful in determining the epidemiology of diseases that are difficult to track and/or control. Copyright<br>Dissertation (MSc)--University of Pretoria, 2012.<br>Veterinary Tropical Diseases<br>unrestricted

Bacillus anthracis is the causal agent of anthrax which primarily affects ungulates, occasionally carnivores and less frequently humans. The endospores of this soil-borne bacterium are highly resistant to extreme conditions, and under ideal conditions, anthrax spores can survive for many years in the soil. The bacterium is generally found in soil at sites where infected animals have died. When these spores are exposed, they have the potential to be ingested by a mammalian species which could lead to an anthrax outbreak. Anthrax is almost never transmitted directly from host to host, but is rather ingested by herbivores while drinking, grazing or browsing in a contaminated environment, with the exception of scavengers and carnivores consuming infected prey. Anthrax is known to be endemic in the northern part of Kruger National Park (KNP) in South Africa (SA), with occasional epidemics spreading southward into the non-endemic areas. The aim of this study is to identify and map areas that are ecologically suitable for the harbouring of B. anthracis spores within the KNP. Anthrax surveillance data and selected environmental variables were used as inputs to the maximum entropy (Maxent) species distribution modelling method. Five-hundred and ninety-seven anthrax occurrence records, dating from the year 1988 to 2011, were extracted from the Skukuza State Veterinary Office’s database. A total of 40 environmental variables were used and their relative contribution to predicting suitability for anthrax occurrence was evaluated using Maxent software (version 3.3.3k). Variables showing the highest gain were then used for subsequent, refined model iterations until the final model parameters were established. The environmental variables that contributed the most to the occurrence of anthrax were soil type, normalized difference vegetation index (NDVI), land type and precipitation. A map was created using a geographic information system (GIS) that illustrates the sites where anthrax spores are most likely to occur throughout the Park. This included the known endemic Pafuri region as well as the low lying soils along the Shingwedzi-Phugwane-Bubube rivers and the Letaba-Olifants river drainage area. The outputs of this study could guide future targeted surveillance efforts to focus on areas predicted to be highly suitable for anthrax, especially since the KNP uses passive surveillance to detect anthrax outbreaks. Knowing where to look can improve sampling efficiency and lead to increased understanding of the ecology of anthrax within the KNP.<br>Dissertation (MMedVet)--University of Pretoria, 2013.<br>Production Animal Studies<br>unrestricted

Thesis (MSc)--Stellenbosch University, 2003.<br>ENGLISH ABSTRACT: The geometric tortoise, Psammobates geometricus, found in the Western Cape, South Africa, is one of the world's rarest tortoises. At present, it is only known from five isolated geographical areas, one being the Worcester-Tulbagh Valley. The primary aims of my study were to determine the status of geometric tortoise subpopulations along the Nl highway in the Worcester Valley, to determine the population structure of these subpopulations and, if necessary, to provide recommendations for the mitigation ofNI highway-induced impacts on geometric tortoise subpopulations along the road. All Renosterveld remnants along the Nl between Du Toitskloof and Worcester were surveyed for the presence of geometric tortoises, and tortoise density and population structure were determined for each remnant that supports tortoises. Two geometric tortoise subpopulations on opposite sides of the Nl were identified. In the larger remnant of the two, females dominated both the subadult and adult classes, whereas in the other remnant male frequency was slightly higher than female frequency for the adult class only. In both remnants, adult females were significantly larger than adult males in mean carapace length. Mean body mass for adult females and males also differed significantly in both remnants. Analysis of habitat quality showed that both remnants supporting the geometric tortoise are seriously infested by encroaching indigenous and alien plants. The barrier effect of the Nl highway can be mitigated by constructing underpasses, linking the two remnants on opposite sides of the road. Furthermore, road mortality of tortoises can be avoided by erecting tortoise proof fencing along appropriate sections of the highway in the Worcester Valley. By linking the remnants, the largest single area available to the geometric tortoise in the Worcester Valley will also be formed. An additional aim of my study was to investigate the correspondence of geometric tortoise density in individual habitat remnants to the West-to-East change in vegetation composition/characteristics occurring along the Nl in the Worcester Valley. Vegetation composition/characteristics were thus determined in all remnants also surveyed for tortoises. The West-to-East change in vegetation composition/characteristics of the study area indicates that there is a transition from Renosterveld in the West to karroid veld in the East.<br>AFRIKAANSE OPSOMMING: Die geometriese skilpad, Psammobates geometrieus, wat in die Wes-Kaap, Suid- Afrika, voorkom, is een van die wêreld se skaarste skilpaaie. Vandag word hierdie skilpad net nog in vyf geïsoleerde geografiese streke gekry, een van hulle die Worcester-Tulbagh Vallei. Die primêre doel van my studie was om die status van geometriese skilpad subpopulasies langs die Nl snelweg in die Worcester Vallei te ondersoek en om die populasiestruktuur van hierdie subpopulasies te bepaal. Verder was die doelook, om aanbevelings te maak oor hoe die impakte van die pad op die geometriese skilpad subpopulasies langs die Nl verminder kan word. Al die Renosterveld oorblyfsels langs die Nl tussen Du Toitskloof en Worcester, en binne die bekende gebied van die geometriese skilpad, is deursoek vir geometriese skilpaaie. Populasiedigtheid en populasiestruktuur van die skilpaaie is bepaal vir elke oorblyfsel wat skilpaaie bevat. Twee geometriese skilpad subpopulasies is gekryaan teenoorgestelde kante van die Nl. In die groter oorblyfsel, het wyfies albei die onvolwasse en volwasse klasse gedomineer. In die ander oorblyfsel is gevind dat die frekwensie van mannetjies ietwat hoër was as die frekwensie van wyfies, maar vir slegs die volwasse kategorie. Binne albei oorblyfsels is gevind dat volwassse wyfies betekenisvol groter in gemiddelde doplengte is as volwasse mannetjies. Gemiddelde liggaamsgewig het ook betekenisvol verskil tussen volwasse wyfies en mannetjies in albei oorblyfsels. 'n Analise van habitatkwaliteit het gewys dat beide oorblyfsels wat die geometriese skilpad ondersteun grootliks ingeneem is deur inheemse indringers, sowel as uitheemse plante. Die versperrings effek van die Nl snelweg kan verminder word deur duikwege te skep wat die twee ooblyfsels, op teenoorgestelde kante van die pad, verbind. Bowendien, deur 'n ondeurdringbare heining langs passende dele van die snelweg in die Worcester Vallei op te rig, sal skilpadmortaliteit vermy word. Deur oorblyfsels te verbind, salook die grootste enkele area, beskibaar aan die geometriese skilpad in die Worcester Vallei, gevorm word. 'n Addisionele doel van hierdie studie was om veranderinge in vegetasie langs 'n wes-na-oos gradient binne die studie area te korreleer met die teenwoordigheid van skilpaaie langs hierdie gradient. Vegetasie samestelling/eienskappe is dus bepaal vir elke oorblyfsel wat deursoek is vir skilpaaie. Die wes-na-oos verandering in vegetasie samestelling/eienskappe binne die studie area wys dat daar 'n oorgang van Renosterveld in die weste na 'n karooagtige veld in die ooste is.

Molecular based species identification is a useful tool in forensic investigations as well as routine work. The ability to cheaply and quickly determine the species of origin of a sample has become increasingly necessary as incidents of wildlife poaching, illegal meat trade and the trade of wildlife products has increased. The current methods of species identification tend to be expensive, time consuming and unreliable. The use of species-specific primers designed to bind to specific areas in the mitochondrial DNA, has been published. This application has been developed for a small number of domestic animals, however the application of these primers to African wildlife species has yet to be done. This method is relatively simple and is based on specific fragment size amplification using polymerase chain reaction and genotyping. A total of thirty seven different species were analyzed with this method and 30 of these species were found to have species-specific fragment sizes. A number of different sample types and conditions were tested including uncommon diagnostic samples such as rhinoceros horn, teeth and claws. In addition, the sensitivity of the method was investigated and determined to be very high, detecting species at a DNA concentration of just 0.1 pg/μl. This method was found to be a highly sensitive, efficient and a fast way to determine species in a number of different sample types and would therefore be of great value in the wildlife trade as these samples can often be of a lower quality or only available in small amounts. The use of this method in forensic science must be done with care due to the problem of cross species amplification. In addition not all of the African Wildlife mammals were available to test, limiting the detection power and specificity of the test. Copyright<br>Dissertation (MSc)--University of Pretoria, 2012.<br>Production Animal Studies<br>unrestricted

Two major goals of managers of Rubondo Island National Park (Rubondo), Tanzania are to increase the number of international visitors to the Park (and thereby increase revenue generated) and to increase support for the Park among residents of nearby communities. I investigated species preferences among children living around Rubondo and the wildlife viewing preferences of international visitors to Tanzania in an attempt to identify flagship species that Park management could use in marketing and outreach campaigns designed to meet their goals. I also assessed local interest in visiting the Park. As local flagships for Rubondo, the Egyptian goose (Alopochen aegyptiacus ) and silver cyprinid (Rastrineobola argentea) appear to have the most potential among the species that I assessed (i.e., fish eagle [Haliaeetus vocifer], Egyptian goose, silver cyprinid, little egret [Egretta garzetta], sitatunga [Tragelaphus speki], genet [Genetta tigrina], monitor lizard [Varanus niloticus], spotted-necked otter [Lutra maculicollis], vervet monkey [Cercopithecus pygerythrus], crocodile [Crocodylus niloticus], hippopotamus [ Hippopotamus amphibius], giraffe [Giraffa camelopardalis ], and elephant [Loxodanta africana]). However, based on either range or limited aesthetic/behavioral appeal, I considered neither species useful as an international flagship. Among species on Rubondo included in investigations of potential international flagships for the Island (including the chimpanzee [Pan troglodytes], crocodile, elephant, giraffe, hippopotamus, vervet monkey, bushbuck, fish eagle, large-spotted genet, goliath heron [Ardea goliath], monitor lizard, sitatunga, and spotted-necked otter), few were concluded to have potential, either because participating visitors had limited "awareness" of and "interest" in viewing the species, local people had strong negative opinions of the species, the animals are not native to the Island, and/or the animals are more easily viewed in more readily-accessible locations. Local people generally had not visited Rubondo but wanted to visit. Not having visited the Park appeared to influence perceptions of the Park, wildlife tourism, and conservation among some participants. Recommendations for local and international marketing and outreach strategies for Rubondo are made based on the outcomes of the aforementioned investigations of local and international species preferences and interest in visiting the Park.

Browse selection, intake, utilisation, palatability, quality and production are tightly linked and need to be considered together in trying to improve our understanding of browsing dynamics and the interactions between browsers and vegetation. Such an understanding is necessary in order to re-evaluate determinations of browser carrying capacities and evaluating actual and potential impacts of browsing animals on vegetation composition and diversity. Browser carrying capacity is determined by both the quantity and the nutritive value of forage. The measurement of browse quantity and nutritive value and the matching of browse supply to browser demand are central to sustainable utilisation and the monitoring of vegetation health. South African savannas are poorly studied with respect to tree canopy growth and browse production making it difficult to quantify the available browse biomass on which browsing capacity estimations are based, and consequently difficult to estimate levels of browsing that are sustainable. This study addressed these issues by investigating browse dynamics, broadly aiming to (1) explore factors affecting browse production, biomass and nutritive value; (2) develop models to assess and monitor these parameters across seasons and properties; (3) use the resultant models in improving our understanding of how to determine browser carrying capacities. More specifically, our study sought to examine the effects of plant physiognomy, forage nutritive value, canopy stratum, defoliation, temperature, rainfall and soil nutrient status on the browse production of evergreen (Carissa bispinosa, Euclea divinorum, Gymnosporia senegalensis), semi-deciduous (Spirostachys africana, Ziziphus mucronata) and deciduous (Acacia nilotica, Dichrostachys cinerea) savanna tree species from June 2003 – June 2005 in three sites along the northern Zululand coastline of KwaZulu-Natal. Available browse biomass, during the dry season, of four key savanna tree species (A. nilotica, E. divinorum, G. senegalensis, and S. africana) was estimated through the development of allometric regression equations. Non-linear regression was used to investigate the relation between the leaf dry mass (LDM) and canopy volume (CVol) of each of the four tree species. Exponential regression (y = a + brlnx) of the natural logarithm of CVol data provided the most accurate and precise description of the tree CVol – LDM relation. A study was undertaken to determine which factors may influence browse production in a southern African savanna. Regression tree models for the browse production identified that the dominant factors influencing browse production were CVol (m3), season, species and height to the lowest leaves of the tree canopy (HL) (m). The length of the growing season had a marked effect on the production potential of savanna tree species, suggesting that improved conditions for growth, i.e. greater rainfall, soil moisture content and improved soil nutrient availability result in a longer period of rapid sustained growth. Species was identified as an important contributing factor to differences in browse production rates, suggesting the need for the development of species or species group models. Mean annual browse production of evergreen trees was greater than that of deciduous and semi-deciduous trees. Mean quarterly (three monthly) browse production was highest, for all trees, during the wet season, with the greatest difference between wet and dry season production being observed in deciduous forms. Evergreen forms showed continuous growth over the whole study, with enhanced growth over the wet season. Deciduous forms, on the other hand, concentrated growth in spurts, when environmental conditions became favourable, with most production occurring during a short growing season. Browse nutritive value was found to be greatest during the wet season, when growth and photosynthesis are at their greatest. Further, browse nutritive value was greatest in deciduous species. Evergreen trees were found to have greater acid detergent fibre (ADF) concentrations than both the deciduous and semi-deciduous trees. By contrast, crude protein (CP) concentrations were greater in semi-deciduous and deciduous species than in evergreen species. The daily CP requirements for maintenance for an adult impala (45 kg) were met by all species over all three study areas and all seasons. Daily CP requirements for growth and lactation, however, were only ever met by deciduous and semi-deciduous species, though this result was not consistent over study areas and seasons. Predictive models for the production of browse on deciduous, semi-deciduous and evergreen trees in northern Zululand were developed using multivariate adaptive regression spline functions. The best predictors of growing season browse production in all three tree guilds (defined here as a group of trees having a characteristic mode of living) were primarily measurable tree dimensions, while the prevailing environmental conditions had little impact. Differences in the production, nutritive value and available browse biomass between the different tree forms and seasons have a profound effect on the determination of browser carrying capacities and need to be incorporated into any game or conservation management plan.<br>Thesis (Ph.D.)-University of KwaZulu-Natal, Pietermaritzburg, 2012.

The existence of the rhino is under serious threat. South Africa has experienced a dramatic increase in rhino poaching since 2008. With the current trend of poaching, rhino in South Africa will soon become extinct. The current strategies to protect rhino have proved to be ineffective. The common view is that rhino poaching is morally wrong, and associated with greed and evil intentions and that rhino poaching must be stopped; however, there are two schools of thought regarding two main approaches (in association with current strategies) to be used in order to stop or limit poaching. The first school of thought supports the CITES ban on international trade in rhino horn, and believes that rhino poaching can only be stopped by eliminating demand (the “conventional approach”). The second school of thought is in favour of the legalisation of international trade, and argues that any solutions to the rhino poaching problem must address the underlying economic forces. This is called the sustainable approach. The research aimed to investigate the effectiveness of the ban on international trade in rhino horn, as well as the viability of legalising international trade. The effectiveness of the current strategies was also investigated. A qualitative research design was used, and eighteen participants were selected in order to obtain information by means of semi-structured interviews and questionnaires.The first school of thought supports the CITES ban on international trade in rhino horn, and believes that rhino poaching can only be stopped by eliminating demand (the “conventional approach”). The second school of thought is in favour of the legalisation of international trade, and argues that any solutions to the rhino poaching problem must address the underlying economic forces. This is called the sustainable approach. The research aimed to investigate the effectiveness of the ban on international trade in rhino horn, as well as the viability of legalising international trade. The effectiveness of the current strategies was also investigated. A qualitative research design was used, and eighteen participants were selected in order to obtain information by means of semi-structured interviews and questionnaires. A literature review was also conducted, for the purpose of evaluating the responses of participants, and also for the purpose of validity and reliability. The findings indicated that the scales tip in favor of a well-managed, legalized trade, inter alia, to make funds available to rhino owners, rhino custodians and the government, on the one hand, and, on the other hand, to reduce the reward and increase the risk to the poacher. Findings also indicated that a legalized trade cannot be implemented in isolation; it goes hand in hand with increased security and improved law enforcement, while strategies such as dehorning and translocation still have a role to play. Finally, findings also indicated that communities need to be involved in the sustainable use approach. These communities are currently excluded from the benefits derived from the neighboring protected areas, thereby making them susceptible to being recruited as poachers.<br>Environmental Sciences<br>Ph. D. (Environmental Management)

The illegal trade in endangered animals in KwaZulu-Natal, with an emphasis on rhino poaching, is tactically addressed in this dissertation. The aim is to expose the nature and extent of these crimes; the victims, offenders and modus operandi involved; the adjudication of wildlife offences; the causes and consequences concerned; the relevant criminological theories to explain these crimes; and recommendations for prevention. This research intends to examine the contemporary pandemic of rhino poaching in KwaZulu-Natal, South Africa, and offer potential techniques for intervention. Furthermore, one of the main goals of the study is to reveal and enhance the extremely neglected field of conservation criminology. A general disregard by society for the environment, as well as the overall ineffectiveness and corruption of criminal justice and conservation authorities, comes to the fore. The purpose of the research is therefore to suggest possible prevention strategies in order to protect the rights of endangered species.<br>Criminology and Security Science<br>M.A. (Criminology)