Auswahl der wissenschaftlichen Literatur zum Thema „Amino acids in animal nutrition“

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Zeitschriftenartikel zum Thema "Amino acids in animal nutrition"

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Böhme, Hartwig. "Amino Acids in Animal Nutrition." Animal Feed Science and Technology 109, no. 1-4 (October 2003): 217. http://dx.doi.org/10.1016/s0377-8401(03)00214-1.

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Harris, P. A. "Amino Acids in Farm Animal Nutrition." British Veterinary Journal 152, no. 6 (November 1996): 737. http://dx.doi.org/10.1016/s0007-1935(96)80150-3.

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Eggum, Bjørn O. "Amino acids in farm animal nutrition. 1994." Livestock Production Science 43, no. 2 (August 1995): 180. http://dx.doi.org/10.1016/0301-6226(95)90012-8.

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Sefer, M., R. B. Petronijevic, D. Trbovic, J. Ciric, T. Baltic, N. Parunovic, and V. Djordjevic. "Amino acids in animal feed: significance and determination techniques." IOP Conference Series: Earth and Environmental Science 854, no. 1 (October 1, 2021): 012082. http://dx.doi.org/10.1088/1755-1315/854/1/012082.

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Abstract Amino acids are fundamental for animal nutrition. Their presence is necessary to maintain the normal structure and function of the intestine, and they are key in regulating metabolic pathways for improving health, survival, growth, development, lactation, and reproduction. The animal feed industry invests great resources and efforts to obtain optimal formulations in which the composition of amino acids plays a key role. In support of these aspirations in recent decades, much attention has been paid to the development and improvement of analytical techniques for the reliable, rapid and
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Mohanty, Bimal, Arabinda Mahanty, Satabdi Ganguly, T. V. Sankar, Kajal Chakraborty, Anandan Rangasamy, Baidyanath Paul, et al. "Amino Acid Compositions of 27 Food Fishes and Their Importance in Clinical Nutrition." Journal of Amino Acids 2014 (October 14, 2014): 1–7. http://dx.doi.org/10.1155/2014/269797.

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Proteins and amino acids are important biomolecules which regulate key metabolic pathways and serve as precursors for synthesis of biologically important substances; moreover, amino acids are building blocks of proteins. Fish is an important dietary source of quality animal proteins and amino acids and play important role in human nutrition. In the present investigation, crude protein content and amino acid compositions of important food fishes from different habitats have been studied. Crude protein content was determined by Kjeldahl method and amino acid composition was analyzed by high perf
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Kutlu, Hasan Rüştü, and Uğur Serbester. "Ruminant Beslemede Son Gelişmeler." Turkish Journal of Agriculture - Food Science and Technology 2, no. 1 (January 11, 2014): 18. http://dx.doi.org/10.24925/turjaf.v2i1.18-37.37.

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One of the most industrialized animal production branches of ruminant production successfully requires a blending of theoretical knowledge of nutritional principles with practical stockmanship, maintaining health and dealing with numbers. It is well known that high yielding, dairy cows, require balanced diet with adequate nutrients for yielding. This is not provided with only a few feedstuffs. Milk production in dairy cows is related to the improvements in genetic merit of farm animals and also developments in feed science, feed technology and animal nutrition. In particular, feeds and feed te
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Grechkina, V. V., E. V. Sheida, and O. V. Kvan. "Microbiome and its association with nutrient metabolism in farm animal nutrition." E3S Web of Conferences 431 (2023): 01027. http://dx.doi.org/10.1051/e3sconf/202343101027.

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This article shows the effect of amino acid preparations on metabolism, nutrient digestibility, and calf development. Essential amino acid mixes in different concentrations (per 1 kg of feed) were introduced into the diet of calves from 9 to 18 months of age: I experimental group - 2 g of lysine + 2 g of methionine + 3 g of threonine + 1 g of tryptophan, II experimental group - 3 g of lysine + 3 g of methionine + 4 g threonine+2 g tryptophan. Calves were calculated on pure amino acids, in % of feed dry matter (per head per day). In calves of the II experimental group there was a significant in
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Yin, Lingqian, Mingxu Xu, Qinke Huang, Donghao Zhang, Zhongzhen Lin, Yan Wang, and Yiping Liu. "Nutrition and Flavor Evaluation of Amino Acids in Guangyuan Grey Chicken of Different Ages, Genders and Meat Cuts." Animals 13, no. 7 (April 2, 2023): 1235. http://dx.doi.org/10.3390/ani13071235.

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The composition and content of amino acids in foodstuffs have a vital impact on the nutritional value and taste. With the aim of understanding the nutrition and flavor of Guangyuan grey chicken, the composition and content of amino acids in the pectoralis and thigh muscle of chickens at the age of 90 d, 120 d and 150 d were determine using liquid chromatography–tandem mass spectrometry (LC-MS/MS) and an amino acid analyzer. A total of 17 amino acids were detected both in pectoralis and thigh muscle via the amino acid analyzer, of which the content of glutamate was the highest. Additionally, 21
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Ilić, Petar, Slađana Rakita, Nedeljka Spasevski, Olivera Đuragić, Ana Marjanović-Jeromela, Sandra Cvejić, and Federica Zanetti. "Nutritive value of Serbian camelina genotypes as an alternative feed ingredient." Food and Feed Research, no. 00 (2022): 25. http://dx.doi.org/10.5937/ffr49-41060.

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Camelina has been used from ancient times, but recently has re-emerged as a valuable plant with the potential for successful replacement of conventional oilseed crops. The utilisation of camelina and its by-products in animal feed is a matter of scientific study due to their excellent nutritional potential. The present study aimed to investigate the nutritive value of two Serbian camelina seed genotypes (NS Zlatka and NS Slatka) as a potential alternative to commonly used oilseed crops in animal feeding. For that purpose proximate composition, fatty acid profile, amino acid profile and tocophe
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Li, Peng, and Guoyao Wu. "Important roles of amino acids in immune responses." British Journal of Nutrition 127, no. 3 (November 15, 2021): 398–402. http://dx.doi.org/10.1017/s0007114521004566.

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AbstractThis commentary highlighted the background, take-home messages, and impacts of our 2007 British Journal of Nutrition paper entitled “Amino acids and immune function”. In 2003–2004, there was an outbreak of severe acute respiratory syndrome (SARS) caused by SARS coronavirus-1 (CoV-1) in Asian countries. By the mid-2000’s, clinical and experimental evidence indicated important roles for amino acids (AA) in improving innate and adaptive immunities in humans and animals. Based on our long-standing interest in AA metabolism and nutritional immunology, we decided to critically analyze advanc
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Dissertationen zum Thema "Amino acids in animal nutrition"

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Nili, Nafisseh. "Limitations to amino acid biosynthesis de novo in ruminal strains of Prevotella and Butyrivibrio." Title page, contents and abstract only, 1996. http://web4.library.adelaide.edu.au/theses/09PH/09phn712.pdf.

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Bibliography: leaves 226-261. Investigates nitrogen utilization in some species of rumen bacteria with the object of understanding the role of ammonia versus exogenous amino acids in relation to microbial growth.
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Zhang, Yongfang. "Amino acid metabolism and requirement in teleost during their early life stages and implications in fish formulated diets." Columbus, Ohio : Ohio State University, 2007. http://rave.ohiolink.edu/etdc/view?acc%5Fnum=osu1199374737.

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Hess, Bret William. "Increasing postruminal amino acid supply to cattle consuming forages /." free to MU campus, to others for purchase, 1996. http://wwwlib.umi.com/cr/mo/fullcit?p9812955.

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Tanner, Sara L. "EVALUATING DIETARY AMINO ACID ADEQUACY IN HORSES USING ISOTOPIC TECHNIQUES." UKnowledge, 2014. http://uknowledge.uky.edu/animalsci_etds/38.

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Little is known about amino acid (AA) requirements in horses despite muscle mass accretion being of importance to an athletic species. Isotope methods for determining AA requirements and whole-body protein synthesis (WBPS) had not been previously used in growing horses. The first study herein was the first to apply isotope methods to determine WBPS in growing horses. In the study, weanling colts received two different levels of crude protein. Whole-body protein kinetics indicated that WBPS was greater when the weanlings were fed the diet with a greater crude protein content (P<0.05). The secon
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Mueller, Andrew L. "The ability of empirical equations based on dilution rate to predict microbial efficiency and amino acid flow post ruminally /." free to MU campus, to others for purchase, 2004. http://wwwlib.umi.com/cr/mo/fullcit?p3144444.

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Kendall, Dustin Clay. "Opportunities and limitations for low-protein diet formulation in swine /." free to MU campus, to others for purchase, 2004. http://wwwlib.umi.com/cr/mo/fullcit?p3164518.

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Scholljegerdes, Eric J. "Amino acid and fatty acid nutrition of beef cattle consuming high-forage diets." Laramie, Wyo. : University of Wyoming, 2005. http://proquest.umi.com/pqdweb?did=888865061&sid=3&Fmt=2&clientId=18949&RQT=309&VName=PQD.

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Ludden, Paul Anthony. "Amino acid and energy interrelationships in growing beef cattle /." free to MU campus, to others for purchase, 1997. http://wwwlib.umi.com/cr/mo/fullcit?p9842550.

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Nolte, Joubert van Eeden. "Essential amino acid requirements for growth in woolled sheep." Thesis, Stellenbosch : University of Stellenbosch, 2006. http://hdl.handle.net/10019.1/1666.

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Thesis (PhD(Agric) (Animal Sciences))--University of Stellenbosch, 2006.<br>This project consisted of five studies. The objectives were to determine the essential amino acid (AA) requirements of growing woolled lambs (Merino and Dohne Merino) and the essential AA profile of duodenal digesta pre-dominantly derived from microbial protein. The limiting essential AA`s in high rumen degradable protein (RDP) diets to growing lambs, where microbial protein is the primary source of AA`s, were also identified. The first study determined the essential AA profile of duodenal protein on a high rumen
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Kamalakar, Rajesh Babu Chiba Lee I. "Effect of the degree and duration of early dietary amino acid restrictions on growth performance, carcass traits, and serum metabolites of pigs, and physical and sensory characteristics of pork." Auburn, Ala, 2008. http://repo.lib.auburn.edu/EtdRoot/2008/FALL/Animal_Sciences/Thesis/Kamalakar_Rajesh_17.pdf.

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Bücher zum Thema "Amino acids in animal nutrition"

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D'Mello, J. P. F., ed. Amino acids in animal nutrition. Wallingford: CABI, 2003. http://dx.doi.org/10.1079/9780851996547.0000.

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Felix, D'Mello J. P., ed. Amino acids in animal nutrition. 2nd ed. Willingford, Oxon, UK: CABI Pub., 2003.

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Felix, D'Mello J. P., ed. Amino acids in farm animal nutrition. Wallingford, Oxon, UK: CAB International, 1994.

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Dorothy, Kroll, and Business Communications Co, eds. Vitamins, minerals, and proteins/amino acids used for animal health. Norwalk, CT: Business Communications Co., 1993.

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International Symposium on Protein Metabolism and Nutrition (5th 1987 Rostock, Germany). 5th International Symposium on Protein Metabolism and Nutrition, Wilhelm-Pieck-University Rostock (GDR). [S.l: s.n., 1988.

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Malcolm, Asplund John, ed. Principles of protein nutrition of ruminants. Boca Raton: CRC Press, 1994.

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Blachier, Francois. Nutritional and Physiological Functions of Amino Acids in Pigs. Vienna: Springer Vienna, 2013.

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B, Ammerman Clarence, Baker David H. 1939-, and Lewis Austin J, eds. Bioavailability of nutrients for animals: Amino acids, minerals, and vitamins. San Diego, Ca: Academic Press, 1995.

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Commission, United States International Trade. Animal feed grade DL-methionine from France: Determination of the Commission in investigation no. 731-TA-255 (preliminary) under the Tariff Act of 1930, together with the information obtained in the investigation. Washington, D.C: U.S. International Trade Commission, 1985.

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United States International Trade Commission. Animal feed grade DL-methionine from France: Determination of the Commission in investigation no. 731-TA-255 (preliminary) under the Tariff Act of 1930, together with the information obtained in the investigation. Washington, D.C: U.S. International Trade Commission, 1985.

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Buchteile zum Thema "Amino acids in animal nutrition"

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Steinberg, Christian E. W. "Nonprotein Amino Acids—‘Fuel at All?’." In Aquatic Animal Nutrition, 243–61. Cham: Springer International Publishing, 2022. http://dx.doi.org/10.1007/978-3-030-87227-4_12.

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Wu, Guoyao. "Chemistry of Protein and Amino Acids." In Principles of Animal Nutrition, 149–92. Boca Raton : Taylor & Francis, 2018.: CRC Press, 2017. http://dx.doi.org/10.1201/9781315120065-4.

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Steinberg, Christian E. W. "Peptides or Amino Acids?—‘The Smaller, the Better?’." In Aquatic Animal Nutrition, 61–77. Cham: Springer International Publishing, 2022. http://dx.doi.org/10.1007/978-3-030-87227-4_4.

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Steinberg, Christian E. W. "Sulfur Amino Acids—‘Much More than Easy Fuel’." In Aquatic Animal Nutrition, 163–92. Cham: Springer International Publishing, 2022. http://dx.doi.org/10.1007/978-3-030-87227-4_9.

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Wu, Guoyao. "Nutrition and Metabolism of Protein and Amino Acids." In Principles of Animal Nutrition, 349–448. Boca Raton : Taylor & Francis, 2018.: CRC Press, 2017. http://dx.doi.org/10.1201/9781315120065-7.

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Steinberg, Christian E. W. "Simple Amino Acids: Gly, Ala, Asp, Gln—‘Pure Easy Fuel?’." In Aquatic Animal Nutrition, 107–15. Cham: Springer International Publishing, 2022. http://dx.doi.org/10.1007/978-3-030-87227-4_6.

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Karau, Andreas, and Ian Grayson. "Amino Acids in Human and Animal Nutrition." In Advances in Biochemical Engineering/Biotechnology, 189–228. Berlin, Heidelberg: Springer Berlin Heidelberg, 2014. http://dx.doi.org/10.1007/10_2014_269.

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Steinberg, Christian E. W. "Basic Amino Acids and Prolines—‘Again: Much More than Easy Fuel’." In Aquatic Animal Nutrition, 193–221. Cham: Springer International Publishing, 2022. http://dx.doi.org/10.1007/978-3-030-87227-4_10.

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Dryden, Gordon McL. "Nutrient requirements." In Fundamentals of applied animal nutrition, 95–110. Wallingford: CABI, 2021. http://dx.doi.org/10.1079/9781786394453.0008.

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Abstract This chapter describes how animals use protein, energy, amino acids, vitamins, minerals and other nutrients and their requirements for these nutrients for physiological processes and production responses.
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He, Wenliang, Peng Li, and Guoyao Wu. "Amino Acid Nutrition and Metabolism in Chickens." In Advances in Experimental Medicine and Biology, 109–31. Cham: Springer International Publishing, 2021. http://dx.doi.org/10.1007/978-3-030-54462-1_7.

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AbstractBoth poultry meat and eggs provide high-quality animal protein [containing sufficient amounts and proper ratios of amino acids (AAs)] for human consumption and, therefore, play an important role in the growth, development, and health of all individuals. Because there are growing concerns about the suboptimal efficiencies of poultry production and its impact on environmental sustainability, much attention has been paid to the formulation of low-protein diets and precision nutrition through the addition of low-cost crystalline AAs or alternative sources of animal-protein feedstuffs. This necessitates a better understanding of AA nutrition and metabolism in chickens. Although historic nutrition research has focused on nutritionally essential amino acids (EAAs) that are not synthesized or are inadequately synthesized in the body, increasing evidence shows that the traditionally classified nutritionally nonessential amino acids (NEAAs), such as glutamine and glutamate, have physiological and regulatory roles other than protein synthesis in chicken growth and egg production. In addition, like other avian species, chickens do not synthesize adequately glycine or proline (the most abundant AAs in the body but present in plant-source feedstuffs at low content) relative to their nutritional and physiological needs. Therefore, these two AAs must be sufficient in poultry diets. Animal proteins (including ruminant meat &amp; bone meal and hydrolyzed feather meal) are abundant sources of both glycine and proline in chicken nutrition. Clearly, chickens (including broilers and laying hens) have dietary requirements for all proteinogenic AAs to achieve their maximum productivity and maintain optimum health particularly under adverse conditions such as heat stress and disease. This is a paradigm shift in poultry nutrition from the 70-year-old “ideal protein” concept that concerned only about EAAs to the focus of functional AAs that include both EAAs and NEAAs.
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Konferenzberichte zum Thema "Amino acids in animal nutrition"

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Grechkina, V. V. "Рroteins and amino acids as the basis of nutrition in animal husbandry". У INTERNATIONAL CONFERENCE “SUSTAINABLE DEVELOPMENT: VETERINARY MEDICINE, AGRICULTURE, ENGINEERING AND ECOLOGY” (VMAEE2022). AIP Publishing, 2023. http://dx.doi.org/10.1063/5.0148388.

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Luo, Fei, Ondrej Halgas, Pratish Gawand, and Sagar Lahiri. "Animal-free protein production using precision fermentation." In 2022 AOCS Annual Meeting & Expo. American Oil Chemists' Society (AOCS), 2022. http://dx.doi.org/10.21748/ntka8679.

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The $1.4 trillion animal industry could not sustainably scale further to feed the next billion population, as it is resource intensive, and heavy in greenhouse gas emission. The recent plant-based food movement has provided solution for more sustainable protein sources. However, the plant-based food sector faces challenges in reaching parity in texture, sensory experience (mouthfeel) and nutritional value as animal products, limiting their potential of reaching beyond the vegan and flexitarian consumers. The technical challenge behind this problem is that proteins from plants have intrinsicall
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Sargsyan, Anyuta, F. Tkhruni, L. Agabekyan, M. Sargsyan, and Arev Israyelyan. "The role of probiotic lab in feedstuff." In 5th International Scientific Conference on Microbial Biotechnology. Institute of Microbiology and Biotechnology, Republic of Moldova, 2022. http://dx.doi.org/10.52757/imb22.31.

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Frequent uncontrolled use of feed antibiotics in intensive production of feedstuff has led to the formation of resistant strains of pathogenic bacteria. The use of feed antibiotics has resulted in increased productivity of farm animals due to the suppression of the pathogenic microflora of the digestive system. The situation is exacerbated by animal stresses due to poor feed quality and poor conditions. Often there are cases of dysbacteriosis, especially in young animals, reduced cows’ reproduction, excess live weight of young animals, increased infectious and alimentary (caused by defective u
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Hu, Bo, David Marks, and Xiao Sun. "Fungal bioprocessing to improve quality of pennycress meal as potential feeding ingredient for monogastric animal." In 2022 AOCS Annual Meeting & Expo. American Oil Chemists' Society (AOCS), 2022. http://dx.doi.org/10.21748/izob6294.

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Pennycress is an annual cover crop in temperate North America and its seeds contain around 30% of oil and 20% of crude protein. Pennycress oil can be used for biodiesel production, while the seed meal has limited use in animal feed due to its relative high content of phenolic compounds and crude fiber. The nutritional value of pennycress meal (PM) can be improved by processing with GRAS fungal strains. In this study, three fungal strains, Rhizopus oryzae (RO), Mucor indicus (MI), and Aspergillus oryzae (AO), were used to ferment PM that contains 21% of total amino acids and 17% of structure ca
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Gutium, Olga, and Viorica Cazac-Scobioala. "Nettles — miraculous plant in traditional dishes." In Simpozion internațional de etnologie: Tradiții și procese etnice, Ediția III. Institute of Cultural Heritage, Republic of Moldova, 2023. http://dx.doi.org/10.52603/9789975841733.09.

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Traditional food plays an important role in local identity, consumer behaviour, the transfer of cultural heritage to future generations and the interaction of this heritage with the rest of the world. Local products are, as a rule, natural products, which have a high nutritional and biological value and are perfectly inscribed in the national food traditions. Until the advent of agriculture, the fi rst foods for our ancestors were those picked up from nature. Th ey had a longer life expectancy and a stronger immune system, and this is probably due to their diet rich in herbs and raw products.
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Resink, J. W., and T. A. T. G. van Kempen. "Protective effects of amino acids after weaning." In 6th EAAP International Symposium on Energy and Protein Metabolism and Nutrition. The Netherlands: Wageningen Academic Publishers, 2019. http://dx.doi.org/10.3920/978-90-8686-891-9_98.

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Yusuf, Muhammad, and Rohanta Siregar. "Analysis of Micro Nutrition and Amino Essential Acids of Pempek Green Content." In First International Conference on Health, Social Sciences and Technology (ICOHSST 2020). Paris, France: Atlantis Press, 2021. http://dx.doi.org/10.2991/assehr.k.210415.054.

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Valadares Filho, S. C., P. M. Amaral, D. Zanetti, L. D. S. Mariz, S. A. Santos, S. A. Lopes, L. F. Prados, et al. "Net essential amino acids requirements for Nellore and crossbred Angus × Nellore cattle." In 6th EAAP International Symposium on Energy and Protein Metabolism and Nutrition. The Netherlands: Wageningen Academic Publishers, 2019. http://dx.doi.org/10.3920/978-90-8686-891-9_151.

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Fernández-Fígares, I., L. Lara, and M. Lachica. "Betaine increases portal appearance of amino acids and peptides in Iberian pigs." In 6th EAAP International Symposium on Energy and Protein Metabolism and Nutrition. The Netherlands: Wageningen Academic Publishers, 2019. http://dx.doi.org/10.3920/978-90-8686-891-9_77.

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Lærke, H. N., A. K. Ingerslev, P. Zhou, J. V. Nørgaard, and S. K. Jensen. "Milk protein – more than just amino acids? A study on growth and growth biomarkers in piglets." In 6th EAAP International Symposium on Energy and Protein Metabolism and Nutrition. The Netherlands: Wageningen Academic Publishers, 2019. http://dx.doi.org/10.3920/978-90-8686-891-9_110.

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Berichte der Organisationen zum Thema "Amino acids in animal nutrition"

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Singh, Anjali. Amino Acids: Building Blocks of Proteins. ConductScience, June 2022. http://dx.doi.org/10.55157/cs20220612.

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Amino acids are essential organic compounds serving as protein building blocks. Recognized for their biological roles, they underpin proteins' structure and interactions. Classified by polarity and nutritional necessity, essential amino acids, not synthesized by the body, include histidine, leucine, lysine, and more, while non-essential ones are produced internally. These molecules exhibit diverse functions, from neurotransmitter precursor synthesis to immune support. Industries leverage amino acids in animal feed, artificial sweeteners, flavor enhancers, and drug manufacturing, highlighting t
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Anderson, Olin D., Gad Galili, and Ann E. Blechl. Enhancement of Essential Amino Acids in Cereal Seeds: Four Approaches to Increased Lysine Content. United States Department of Agriculture, October 1998. http://dx.doi.org/10.32747/1998.7585192.bard.

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Cereal seeds are the basis of the human diet, and their amino acid composition is thus of major nutritional and economic importance. Currently, deficiencies in essential amino acids are addressed, when possible, by additionalprotein sources or by supplementing animal feed with non-cereal protein or synthetic amino acids. A number of strategies have been suggested to make cereal flours more complete and balanced sources of amino acids, although systematic examination of such strategies is rare. This project proposed to begin such a systematic examination using four complementary and parallel ap
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Groot, J. J., J. Broeze, and R. B. Castelein. Food and nutrition security in Kibera (Nairobi, Kenya) with a focus on protein and amino acids. Wageningen: Wageningen Food & Biobased Research, 2023. http://dx.doi.org/10.18174/583746.

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McGuire, Mark A., Amichai Arieli, Israel Bruckental, and Dale E. Bauman. Increasing Mammary Protein Synthesis through Endocrine and Nutritional Signals. United States Department of Agriculture, January 2001. http://dx.doi.org/10.32747/2001.7574338.bard.

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Objectives To determine endocrine factors that regulate the partitioning of amino acids by the mammary gland. To evaluate dietary flow and supply of energy and amino acids and their effects on milk protein synthesis and endocrine status. To use primary cultures of cow mammary epithelial cells to examine the role of specific factors on the rates and pattern of milk protein synthesis. Milk protein is an increasingly valuable component of milk but little is known regarding the specific hormonal and nutritional factors controlling milk protein synthesis. The research conducted for this project has
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Sadot, Einat, Christopher Staiger, and Mohamad Abu-Abied. Studies of Novel Cytoskeletal Regulatory Proteins that are Involved in Abiotic Stress Signaling. United States Department of Agriculture, September 2011. http://dx.doi.org/10.32747/2011.7592652.bard.

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In the original proposal we planned to focus on two proteins related to the actin cytoskeleton: TCH2, a touch-induced calmodulin-like protein which was found by us to interact with the IQ domain of myosin VIII, ATM1; and ERD10, a dehydrin which was found to associate with actin filaments. As reported previously, no other dehydrins were found to interact with actin filaments. In addition so far we were unsuccessful in confirming the interaction of TCH2 with myosin VIII using other methods. In addition, no other myosin light chain candidates were found in a yeast two hybrid survey. Nevertheless
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