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Zeitschriftenartikel zum Thema „Bacteriophages“

Autor: Grafiati

Veröffentlicht am 4. Juni 2021

Zuletzt aktualisiert am 25. Juli 2025

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1

Samir, Safia. "Bacteriophages as Therapeutic Agents: Alternatives to Antibiotics." Recent Patents on Biotechnology 15, no. 1 (2021): 25–33. http://dx.doi.org/10.2174/1872208315666210121094311.

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: Bacteriophages are bacterio-specific viruses that constitute the main portion of the environment. Bacteriophages inject their genome into the targeted bacterial cells and some of them can disrupt the metabolism of bacteria and cause bacterial cell disintegration. The application of bacteriophages to kill bacteria is known as bacteriophage therapy. Since bacteriophages target bacteria and are strain-specific, every bacteriophage/bacterial host pair is unique. They are believed to cause no harm to humans. An additional advantage of the strain-specific nature of bacteriophages is that they do n

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Putra, Ramendra Dirgantara, and Diana Lyrawati. "Interactions between Bacteriophages and Eukaryotic Cells." Scientifica 2020 (June 9, 2020): 1–8. http://dx.doi.org/10.1155/2020/3589316.

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As the name implies, bacteriophage is a bacterium-specific virus. It infects and kills the bacterial host. Bacteriophages have gained attention as alternative antimicrobial entities in the science community in the western world since the alarming rise of antibiotic resistance among microbes. Although generally considered as prokaryote-specific viruses, recent studies indicate that bacteriophages can interact with eukaryotic organisms, including humans. In the current review, these interactions are divided into two categories, i.e., indirect and direct interactions, with the involvement of bact

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Manrique, Pilar, Benjamin Bolduc, Seth T. Walk, John van der Oost, Willem M. de Vos, and Mark J. Young. "Healthy human gut phageome." Proceedings of the National Academy of Sciences 113, no. 37 (2016): 10400–10405. http://dx.doi.org/10.1073/pnas.1601060113.

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The role of bacteriophages in influencing the structure and function of the healthy human gut microbiome is unknown. With few exceptions, previous studies have found a high level of heterogeneity in bacteriophages from healthy individuals. To better estimate and identify the shared phageome of humans, we analyzed a deep DNA sequence dataset of active bacteriophages and available metagenomic datasets of the gut bacteriophage community from healthy individuals. We found 23 shared bacteriophages in more than one-half of 64 healthy individuals from around the world. These shared bacteriophages wer

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Maura, Damien, Matthieu Galtier, Chantal Le Bouguénec, and Laurent Debarbieux. "Virulent Bacteriophages Can Target O104:H4 Enteroaggregative Escherichia coli in the Mouse Intestine." Antimicrobial Agents and Chemotherapy 56, no. 12 (2012): 6235–42. http://dx.doi.org/10.1128/aac.00602-12.

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ABSTRACTIn vivobacteriophage targeting of enteroaggregativeEscherichia coli(EAEC) was assessed using a mouse intestinal model of colonization with the O104:H4 55989Str strain and a cocktail of three virulent bacteriophages. The colonization model was shown to mimic asymptomatic intestinal carriage found in humans. The addition of the cocktail to drinking water for 24 h strongly decreased ileal and weakly decreased fecal 55989Str concentrations in a dose-dependent manner. These decreases in ileal and fecal bacterial concentrations were only transient, since 55989Str concentrations returned to t

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SAKMANOĞLU, Aslı, та Hasan Hüseyin HADİMLİ. "Typing of ΦSP–3 lytic Salmonella bacteriophages obtained from various fecal sources". TURKISH JOURNAL OF VETERINARY AND ANIMAL SCIENCES 44, № 5 (2020): 1047–54. http://dx.doi.org/10.3906/vet-2005-105.

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Although several reports are available on both ΦSP–1 and ΦSP–3 lytic Salmonella bacteriophages obtained from poultry, further research is required to study the effectiveness of ΦSP–3 type on serovars isolated from other sources. In the present study, we aimed to isolate bacteriophages from 8 serovars previously obtained from 869 fecal samples (calf, dairy cow, buffalo, and camel), genotype the bacteriophages, and detect the cross-lytic activities of the bacteriophages on Salmonella enterica subsp. enterica serovar Kentucky, S.Anatum, and S.Muenchen. A total of 16 bacteriophages were detected a

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Ariyanti, Tati. "The Use of Bacteriophage for Detection and Biocontrol of Foodborne Pathogen." Indonesian Bulletin of Animal and Veterinary Sciences 28, no. 1 (2018): 33. http://dx.doi.org/10.14334/wartazoa.v28i1.1791.

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Bacteriophages are viruses that have ability to attack bacterial cells in specific receptors, infect, multiply in bacterial cells and eventually lyse bacterial cells. This unique bacteriophage character is highly beneficial because it is harmless to mammalian cells and does not interfere with natural microbes. Bacteriophages are easy to obtain because they are widespread in the environment such as soil, water, animal, and farm waste or food. This paper describes the potential use of bacteriophages to detect pathogen and foodborne pathogen biocontrol. Bacteriophages are very potential to contro

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Moon, Choi, Jeong, Sohn, Han, and Oh. "Research Progress of M13 Bacteriophage-Based Biosensors." Nanomaterials 9, no. 10 (2019): 1448. http://dx.doi.org/10.3390/nano9101448.

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Recently, new virus-based sensor systems that operate on M13 bacteriophage infrastructure have attracted considerable attention. These systems can detect a range of chemicals with excellent sensitivity and selectivity. Filaments consistent with M13 bacteriophages can be ordered by highly established forms of self-assembly. This allows M13 bacteriophages to build a homogeneous distribution and infiltrate the network structure of nanostructures under mild conditions. Phage display, involving the genetic engineering of M13 bacteriophages, is another strong feature of the M13 bacteriophage as a fu

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TARAKANOV, R. I., A. N. IGNATOV, and F. S. DZHALILOV. "ISOLATION OF SPECIFIC BACTERIOPHAGES - PSEUDOMONAS SAVASTANOI PV. GLYCINEA - AND THEIR USE IN SOYBEAN BACTERIAL BLIGHT CONTROL." Izvestiâ Timirâzevskoj selʹskohozâjstvennoj akademii, no. 4 (2020): 43–53. http://dx.doi.org/10.26897/0021-342x-2020-4-43-53.

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Bacterial blight is one of most harmful diseases of legumes, reducing the profitability of soybean production in Russian Federation. Among a number of Pseudomonas isolates obtained from diseased seeds and plants of soybean, 4 strains were selected and confirmed as Pseudomonas savastanoipv. glycinea (Psg). Properties of the isolated bacteria were similar to type strain of Psg CFBP 2214 in plant virulence, LOPAT tests, and PCR analysis for coronafacate ligase gene, and partly – in the phage reaction profile. Four isolates of bacteriophages specific to Psg were obtained from soil samples taken fr

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Xu, Anqi. "Draft Of Bacteriophage Therapy Review." Highlights in Science, Engineering and Technology 74 (December 29, 2023): 1105–16. http://dx.doi.org/10.54097/hfwfxj54.

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Phage therapy, which uses bacterial viruses (bacteriophages) to treat bacterial infections. In recent years, the issue of antibiotic resistance in various bacteria has become prominent, and the effectiveness of antibiotic drugs has generally declined, which has re raised people's scrutiny of bacteriophage therapy. Traditionally, bacteriophage therapy relies on the use of natural bacteriophages to infect and dissolve bacteria at the site of infection. Current research on the use of bacteriophages and their lytic proteins to combat multidrug-resistant bacterial infections suggests that bacteriop

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Florent, Perrine, Henry-Michel Cauchie, Malte Herold, Stéphan Jacquet, and Leslie Ogorzaly. "Soil pH, Calcium Content and Bacteria as Major Factors Responsible for the Distribution of the Known Fraction of the DNA Bacteriophage Populations in Soils of Luxembourg." Microorganisms 10, no. 7 (2022): 1458. http://dx.doi.org/10.3390/microorganisms10071458.

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Bacteriophages participate in soil life by influencing bacterial community structure and function, biogeochemical cycling and horizontal gene transfer. Despite their great abundance, diversity, and importance in microbial processes, they remain little explored in environmental studies. The influence of abiotic factors on the persistence of bacteriophages is now recognized; however, it has been mainly studied under experimental conditions. This study aimed to determine whether the abiotic factors well-known to influence bacteriophage persistence also control the natural distribution of the know

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Raees, Fahad, Azian Harun, Abdalla Ahmed, and Zakuan Zainy Deris. "Potential Usefulness of Bacteriophages for the Treatment of Multidrug-Resistant Acinetobacter Infection." Malaysian Journal of Medical Sciences 30, no. 5 (2023): 7–22. http://dx.doi.org/10.21315/mjms2023.30.5.2.

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Bacteriophages were discovered in early 20th century. However, the interest in bacteriophage research was reduced with the discovery of antibiotics. With the increasing number of infections due to multidrug-resistant (MDR) organisms, the potential usefulness of bacteriophages as therapeutic agents has been re-evaluated. In this review, we found that more than 30 lytic bacteriophages that infect Acinetobacter species have been characterised. These are mainly members of Caudovirales, with genome sizes ranging from 31 kb to 234 kb and G+C contents ranging from 33.5% to 45.5%. The host range can b

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Gorshenin, Aleksandr Vladimirovich. "Participation of microbiologists Z.V. Ermolyeva and L.M. Yakobson in a scientific discussion about the fate of the production of Soviet cholera bacteriophages in 1967." Samara Journal of Science 10, no. 4 (2021): 201–7. http://dx.doi.org/10.17816/snv2021104211.

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Since the late 1920s in the USSR, along with many countries, there has been an interest in studying the phenomenon of bacteriophagy and its use in medicine. Bacteriophages are called bacterial viruses. Significant work on the study of bacteriophages and their use for medical purposes was carried out by Soviet microbiologists Zinaida Vissarionovna Ermolyeva and Lidiya Mikhailovna Yakobson. They paid especially great attention to the study of cholera bacteriophage, which during the Great Patriotic War helped prevent the cholera epidemic in frontline Stalingrad. In the 1940s due to the advent of

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Musin, Egor V., Aleksandr L. Kim, Alexey V. Dubrovskii, Elena V. Ariskina, Ekaterina B. Kudryashova, and Sergey A. Tikhonenko. "The Pathways to Create Containers for Bacteriophage Delivery." Polymers 14, no. 3 (2022): 613. http://dx.doi.org/10.3390/polym14030613.

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Antimicrobial resistance is a global public health threat. One of the possible ways to solve this problem is phage therapy, but the instability of bacteriophages hinders the development of this approach. A bacteriophage delivery system that stabilizes the phage is one of the possible solutions to this problem. This study is dedicated to exploring methods to create encapsulated forms of bacteriophages for delivery. We studied the effect of proteolytic enzymes on the destruction of the polyelectrolyte microcapsule shell and revealed that protease from Streptomyces griseus was able to destroy the

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Levanova, L. A., Yu V. Zakharova, A. A. Markovskaya, and L. Yu Otdushkina. "Bacteriophage sensitivity of opportunistic microbiota in children with gut dysbiosis." Fundamental and Clinical Medicine 7, no. 3 (2022): 40–45. http://dx.doi.org/10.23946/2500-0764-2022-7-3-40-45.

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Aim. As the activity of bacteriophages is species- and strain-specific, it is necessary to study bacteriophage sensitivity in distinct geographic regions with various disease patterns. Here, we aimed to study the lytic activity of specific commercially available bacteriophages against Klebsiella spp., Proteus spp., and Staphylococcus aureus isolated from the intestines of children with gut dysbiosis.Materials and Methods. Bacteriophage sensitivity was assessed in 315 opportunistic microorganisms (125 Staphylococcus aureus strains, 120 Klebsiella spp. strains, 70 Proteus spp. strains) isolated

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Khan, M. A., H. Satoh, T. Mino, H. Katayama, F. Kurisu, and T. Matsuo. "Bacteriophage-host interaction in the enhanced biological phosphate removing activated sludge system." Water Science and Technology 46, no. 1-2 (2002): 39–43. http://dx.doi.org/10.2166/wst.2002.0453.

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Bacteriophages were isolated from a laboratory scale enhanced biological phosphate removing (EBPR) activated sludge process, and their host range was examined. Bacterial isolates to host the bacteriophages were isolated from the EBPR activated sludge process. Bacteriophages were eluted from the EBPR activated sludge, enriched by incubation with the bacterial isolates, and then tested for plaque formation on each of the bacterial isolates. Out of 12 bacterial isolates isolated, 4 supported plaque formation. Four bacteriophages were obtained from the plaques. The host range test was conducted wi

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Kamtaeja, Somboon, Srinuan Niyom, and Nanthanit Jaruseranee. "Isolation of Biofilm Removing Bacteriophages from the Hot Spring of Mae Chan Fault, Chiang Rai, Northern Thailand." Chiang Mai Journal of Science 51, no. 1 (2024): 1–10. http://dx.doi.org/10.12982/cmjs.2024.009.

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T errestrial hot springs offer unique habitats for various bacteriophages, revealing potential reservoirs and a library for antibiotic-resistant and biofilm-removing bacteriophages. This study aimed to identify biofilm-removing bacteriophages as a potential method to eradicate antibiotic-resistant bacteria. Samples were collected from the Mae Chan Fault geothermal sites in Chiang Rai province, north of Thailand. Our bacteriophages, HSPT1 and HSPT2, effectively infect Pseudomonas aeruginosa TISTR1287, as measured by their plaque sizes (3.23 ± 0.26 mm) and (1.14 ± 0.18 mm), respectively. Thermos

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Li, Jinyu, and John J. Dennehy. "Differential Bacteriophage Mortality on Exposure to Copper." Applied and Environmental Microbiology 77, no. 19 (2011): 6878–83. http://dx.doi.org/10.1128/aem.05661-11.

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ABSTRACTMany studies report that copper can be used to control microbial growth, including that of viruses. We determined the rates of copper-mediated inactivation for a wide range of bacteriophages. We used two methods to test the effect of copper on bacteriophage survival. One method involved placing small volumes of bacteriophage lysate on copper and stainless steel coupons. Following exposure, metal coupons were rinsed with lysogeny broth, and the resulting fluid was serially diluted and plated on agar with the corresponding bacterial host. The second method involved adding copper sulfate

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Kargina, T. M., E. I. Sakanyan, D. S. Davydov, and R. L. Parfenyuk. "Elaboration of Pharmacopoeial Quality Standards for Bacteriophage Products." BIOpreparations. Prevention, Diagnosis, Treatment 19, no. 4 (2019): 233–41. http://dx.doi.org/10.30895/2221-996x-2019-19-4-233-241.

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Bacteriophages are novel safe and efficacious medicinal products that are used for treatment of intestinal infections and purulent inflammations. The fact that virulent phages can be adapted to fight antibiotic-resistant bacterial strains makes this group of medicines a promising means of treatment of infections associated with medical interventions. The elaboration of quality standards for bacteriophage products will enable alignment of the quality requirements and test methods. There are no monographs on bacteriophage products in pharmacopoeias of other countries, therefore, the development

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Bach, S. J., R. P. Johnson, K. Stanford, and T. A. McAllister. "Bacteriophages reduce Escherichia coli O157:H7 levels in experimentally inoculated sheep." Canadian Journal of Animal Science 89, no. 2 (2009): 285–93. http://dx.doi.org/10.4141/cjas08083.

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Bacteriophage biocontrol has potential as a means of mitigating the prevalence of Escherichia coli O157:H7 in ruminants. The efficacy of oral administration of bacteriophages for reducing fecal shedding of E. coli O157:H7 by sheep was evaluated using 20 Canadian Arcott rams (50.0 ± 3.0) housed in four rooms (n = 5) in a contained facility. The rams had ad libitum access to drinking water and a pelleted barley-based total mixed ration, delivered once daily. Experimental treatments consisted of administration of E. coli O157:H7 (O157), E. coli O157:H7+bacteriophages (O157+phage), bacteriophages

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Reyneke, Brandon, Benjamin Havenga, Monique Waso-Reyneke, Sehaam Khan, and Wesaal Khan. "Benefits and Challenges of Applying Bacteriophage Biocontrol in the Consumer Water Cycle." Microorganisms 12, no. 6 (2024): 1163. http://dx.doi.org/10.3390/microorganisms12061163.

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Bacteria (including disinfection- and antibiotic-resistant bacteria) are abundant in the consumer water cycle, where they may cause disease, and lead to biofouling and infrastructure damage in distributions systems, subsequently resulting in significant economic losses. Bacteriophages and their associated enzymes may then offer a biological control solution for application within the water sector. Lytic bacteriophages are of particular interest as biocontrol agents as their narrow host range can be exploited for the targeted removal of specific bacteria in a designated environment. Bacteriopha

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Adhikari, Niran, and Krishna P. Acharya. "Effectiveness of Bacteriophage Therapy in Field Conditions and Possible Future Applications." Current Pharmaceutical Biotechnology 21, no. 5 (2020): 364–73. http://dx.doi.org/10.2174/1389201021666191217111156.

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Background: Bacteriophages are viruses, which are obligate parasites of specific bacteria for the completion of their lifecycle. Bacteriophages could be the possible alternative to antibioticresistant bacterial diseases. With this objective, extensive research in different fields is published which are discussed in this article. Results: After a review of bacteriophage therapy, bacteriophages were found to be effective against the multidrug-resistant bacteria individually or synergistically with antibiotics. They were found to be more effective, even better than the bacteria in the development

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Kochetova, T. A., V. V. Yuskevich, F. M. Zurabov, G. T. Sadykova, P. V. Medvedev, and V. M. Popova. "Isolation of novel <i>Actinomyces oris</i> and <i>Aggregatibacter actinomycetemcomitans</i> bacteriophages and study of their biological characteristics <i>in vitro</i>." Biological Products. Prevention, Diagnosis, Treatment 24, no. 1 (2024): 103–16. http://dx.doi.org/10.30895/2221-996x-2024-24-1-103-116.

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SCIENTIFIC RELEVANCE. The incidence of periodontal infections with antibiotic-resistant strains of Actinomyces oris and Aggregatibacter actinomycetemcomitans capable of forming biofilms in the oral cavity is increasing, and the effectiveness of antimicrobials against biofilms is insufficient. Therefore, the isolation of bacteriophages active against A. oris and Ag. actinomycetemcomitans is an urgent task.AIM. This study aimed to isolate bacteriophages active against A. oris and Ag. actinomycetemcomitans, study their biological properties, and select optimum culture conditions providing pure li

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Nishiyama, Hiroki, Hisashi Endo, Romain Blanc-Mathieu, and Hiroyuki Ogata. "Ecological Structuring of Temperate Bacteriophages in the Inflammatory Bowel Disease-Affected Gut." Microorganisms 8, no. 11 (2020): 1663. http://dx.doi.org/10.3390/microorganisms8111663.

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The aim of this study was to elucidate the ecological structure of the human gut temperate bacteriophage community and its role in inflammatory bowel disease (IBD). Temperate bacteriophages make up a large proportion of the human gut microbiota and are likely to play a role in IBD pathogenesis. However, many of these bacteriophages await characterization in reference databases. Therefore, we conducted a large-scale reconstruction of temperate bacteriophage and bacterial genomes from the whole-metagenome sequence data generated by the IBD Multi’omics Database project. By associating phages with

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Roseline, Felicia, Diana Elizabeth Waturangi, and Yogiara Y. "ISOLATION, CHARACTERIZATION, AND APPLICATION OF BACTERIOPHAGES AGAINST SEVERAL FOOD SPOILAGE BACTERIA: Bacillus subtilis, Bacillus cereus, AND Shewanella Putrefaciens." Bacterial Empire 4, no. 2 (2021): e263. http://dx.doi.org/10.36547/be.263.

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Introduction: Bacteriophages can be the natural alternative method to prevent food spoilage caused by bacteria. This research was conducted to isolate bacteriophages from a soil sample, retail food, and wastewater from fish and then the bacteriophages will be characterized for their activity against several food spoilage bacteria, such as Bacillus cereus, Bacillus subtilis, and Shewanella putrefaciens and will be further investigated for application as food preservation. Experimental: B. cereus (Atma Jaya culture collection), B. subtilis ATCC 6633, and S. putrefaciens ATCC 8071 were used for b

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Hu, Yang, Bader Al Shaaer, Lu Liang, and Ian F. Connerton. "Campylobacter Bacteriophage Infection at Refrigeration Temperatures." Applied Microbiology 3, no. 4 (2023): 1392–406. http://dx.doi.org/10.3390/applmicrobiol3040094.

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The application of bacteriophages to control foodborne bacterial pathogens in foods has gained traction in recent years. Poultry meat is a major source of Campylobacter jejuni, and a target for the application of bacteriophages. To offer the prospect of a post-harvest control measure, the bacteriophage must function at refrigeration temperatures, where C. jejuni does not grow but can survive. Here, we report actions of three classes of Campylobacter bacteriophage at 4 °C. The pre-incubation of broth cultures at 4 °C before a shift to 42 °C under conditions that support the growth of the host b

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Shruti, Sinha, and Srivastava* Soumya. "Bacteriophage and Phage-Therapy: An Alternative to Antibiotics." eLifePress 1, no. 1 (2020): 21–27. https://doi.org/10.5281/zenodo.4307923.

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Bacteriophages are known for a century but their use in therapy to cure bacterial infection is still unknown. The working on bacteriophage investigation started about a century ago with their discovery by the English microbiologist Twort in 1915. The continuous growth of resistance in bacteria for various diseases and infections has caused renewing in bacteriophage therapy against bacterial infections. Lytic bacteriophages are those bacteriophages that can kill bacteria that are resistant to antibiotics by the end of their lytic cycle. Bacteriophages use proteins for the lysis of bacteria, ter

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Khan, Muhammad Fayaz, Aamer Ali Khattak, Afshan Saleem, Muhammad Rizwan, Muhammad Asif, and Iqbal Ahmad Alvi. "Efficient Reduction of Pseudomonas aeruginosa Biofilms Using the Myoviridae Lytic Bacteriophage vBPaeM MLG." Lahore Garrison University Journal of Life Sciences 7, no. 03 (2023): 262–79. http://dx.doi.org/10.54692/lgujls.2023.0703275.

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Pseudomonas aeruginosa biofilm infections pose significant challenges in clinical settings due to their increased resistance to conventional antibiotics. Bacteriophages, viruses that infect and kill bacteria, have emerged as promising agents for combating biofilm-related infections. This study aimed to isolate and characterize a potent bacteriophage with antibiofilm activity against P. aeruginosa. Hospital sewage was utilized to isolate a bacteriophage targeting P. aeruginosa. Quantification of phages was conducted through spot tests and doublelayer agar methods. The stability of the isolated

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Brišar, Nuša, Katja Šuster, Simona Kranjc Brezar, Robert Vidmar, Marko Fonović, and Andrej Cör. "An Engineered M13 Filamentous Nanoparticle as an Antigen Carrier for a Malignant Melanoma Immunotherapeutic Strategy." Viruses 16, no. 2 (2024): 232. http://dx.doi.org/10.3390/v16020232.

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Bacteriophages, prokaryotic viruses, hold great potential in genetic engineering to open up new avenues for vaccine development. Our study aimed to establish engineered M13 bacteriophages expressing MAGE-A1 tumor peptides as a vaccine for melanoma treatment. Through in vivo experiments, we sought to assess their ability to induce robust immune responses. Using phage display technology, we engineered two M13 bacteriophages expressing MAGE-A1 peptides as fusion proteins with either pVIII or pIIII coat proteins. Mice were intraperitoneally vaccinated three times, two weeks apart, using two differ

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Rai, Akanksha, and Krishna Khairnar. "Initial Survey of Staphylococcus Bacteriophages and Simple Modifications in the Enrichment Method for Enhancing Plaques." Research Journal of Biotechnology 17, no. 7 (2022): 130–33. http://dx.doi.org/10.25303/1707rjbt1300133.

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Isolation of bacteriophages against Staphylococcus aureus is difficult when compared to the bacteriophages of other genera. We did a preliminary survey for the presence of bacteriophages of S. aureus and Escherichia coli in the environmental water samples. We did not get bacteriophages against S. aureus, but bacteriophages against E. coli were ample. Subsequently, a river-water sample was taken and through the enrichment method with some modifications, we got a bacteriophage against a methicillin-resistant S. aureus. Here, we are reporting simple changes in the enrichment method to enhance pla

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Kurtböke, İpek, Nina Chanishvili, and Jeremy J. Barr. "Bacteriophages." Microbiology Australia 40, no. 1 (2019): 3. http://dx.doi.org/10.1071/ma19002.

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In order to avoid a return to the pre-antibiotic era, alternative treatments to combat microbial diseases are urgently needed. In this context, bacteriophages, which have been used effectively in distant parts of the world during the cold war era, are now gaining significant interest in the West. This special issue of the Microbiology Australia thus focusses on bacteriophages with contributions from Australia and from the members of the Expert round table on acceptance and re-implementation of bacteriophage therapy.

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Kharaeva, Z. F., M. Sh Mustafaev, L. Z. Blieva, E. B. Barokova, S. M. Mustafaeva, and S. A. Dyshekova. "Evaluation of sensitivity to bacteriophages of strains isolated from children with congenital malformations of the maxillofacial region." REPORTS ADYGE (CIRCASSIAN) INTERNATIONAL ACADEMY OF SCIENCES 20, no. 1 (2020): 40–45. http://dx.doi.org/10.47928/1726-9946-2020-20-1-40-45.

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Bacteriophage are used in clinical practice along with antibiotics. It is known that in many cases phage drugs are superior to other antibacterial drugs in their activity against antibiotic-resistant pathogens. Bacteriophages do not cause toxic or allergic side effects and have no contraindications.The use of bacteriophage preparations stimulates the activation of adaptive and innate immune factors, so phage therapy is particularly effective in the treatment of chronic inflammatory diseases against the background of immunosuppressive States. Bacteriophages do not interfere with the implementat

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Sjahriani, Tessa, Eddy Bagus Wasito, and Wiwiek Tyasningsih. "Isolation and Identification of Escherichia coli O157:H7 Lytic Bacteriophage from Environment Sewage." International Journal of Food Science 2021 (August 11, 2021): 1–11. http://dx.doi.org/10.1155/2021/7383121.

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Escherichia coli O157:H7 is one of the pathogenic bacteria causing foodborne disease. The use of lytic bacteriophages can be a good solution to overcome the disease. This study is aimed at isolating lytic bacteriophages from environmental sewage with E. coli O157:H7 bacterial cells. The sample used in this study was eight bacteriophages, and the technique used in identifying E. coli O157:H7 carriers of the stx1 and stx2 genes was PCR. The double layer plaque technique was used to classify bacteriophages. Plaque morphology, host specificity, and electron micrograph were used to identify the bac

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Baqer, Abeer Ameen, Kokxin Fang, Norfarhan Mohd-Assaad, Siti Noor Adnalizawati Adnan, and Norefrina Shafinaz Md Md Nor. "In Vitro Activity, Stability and Molecular Characterization of Eight Potent Bacteriophages Infecting Carbapenem-Resistant Klebsiella Pneumoniae." Viruses 15, no. 1 (2022): 117. http://dx.doi.org/10.3390/v15010117.

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Background: Members of the genus Klebsiella are among the leading microbial pathogens associated with nosocomial infection. The increased incidence of antimicrobial resistance in these species has propelled the need for alternate/combination therapeutic regimens to aid clinical treatment, including bacteriophage therapy. Bacteriophages are considered very safe and effective in treating bacterial infections. In this study, we characterize eight lytic bacteriophages that were previously isolated by our team against carbapenem-resistant Klebsiella pneumoniae. Methods: The one-step-growth curves,

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Monarchovich, M. A., O. A. Galaburda, A. A. Arashkova, N. V. Sverchkova, and E. I. Kolomiets. "ISOLATION AND CHARACTERISTICS OF BACTERIOPHAGES SANITARY-INDICATIVE MICROORGANISMS." Eurasian Journal of Applied Biotechnology, no. 2 (July 9, 2022): 25–32. http://dx.doi.org/10.11134/btp.2.2022.4.

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Isolated phages showed activity toward opportunistic and pathogenic bacteria of genera Escherichia (K3P1, K3P2, BIM BV-44 D) and Pseudomonas (PsP1), formed lysis zones shaped as negative homogeneous colonies with a clear-cut even edge (K3P1, PsP1, BIM BV -44 D), or negative colonies with a peripheral zone of partial lysis (K3P2). The diameter of phage plaques varied from 0,5–1,5 mm. Lytic activity spectrum of phage isolates was investigated. It was found that the studied bacteriophages displayed species specificity. Bacteriophages K3P1, K3P2 were distinguished by the highest lytic range. Diffe

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OTTER, JONATHAN A., and ANDREA BUDDE-NIEKIEL. "Hydrogen Peroxide Vapor: A Novel Method for the Environmental Control of Lactococcal Bacteriophage†." Journal of Food Protection 72, no. 2 (2009): 412–14. http://dx.doi.org/10.4315/0362-028x-72.2.412.

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Bacteriophage contamination can be problematic, especially in industrial settings. We examined the in vitro efficacy of hydrogen peroxide vapor (HPV) for the inactivation of two lactococcal bacteriophages dried onto stainless steel discs. A more than 6-log reduction was achieved on both bacteriophages compared with unexposed controls by 50 min of HPV exposure in an isolator. HPV might be useful for the environmental control of bacteriophages.

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Chen, Jun, Jiajun Han, Zheng Yang, et al. "Bacteriophages as Potential Anti-Pathogenic Agents for Intestinal Health of Weaned Piglets in the Post-Antibiotic Era: An Updated Review." Animals 15, no. 12 (2025): 1713. https://doi.org/10.3390/ani15121713.

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Background: The use of in-feed antibiotics was banned in numerous countries within the animal production industry as a result of the emergence of antibiotic-resistant bacteria and the presence of residual antibiotics. Bacteriophages, which are viruses that infect host bacterial cells, are considered the natural predators of bacteria. Over the past two decades, bacteriophages have garnered increasing attention for their potential in controlling pathogenic bacteria in weaned piglets. Aims: The aim of this overview was to update the progress of bacteriophage application in weaned piglets. Methods

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Doore, Sarah M., Jason R. Schrad, William F. Dean, John A. Dover, and Kristin N. Parent. "ShigellaPhages Isolated during a Dysentery Outbreak Reveal Uncommon Structures and Broad Species Diversity." Journal of Virology 92, no. 8 (2018): e02117-17. http://dx.doi.org/10.1128/jvi.02117-17.

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ABSTRACTIn 2016, Michigan experienced the largest outbreak of shigellosis, a type of bacillary dysentery caused byShigellaspp., since 1988. Following this outbreak, we isolated 16 novelShigella-infecting bacteriophages (viruses that infect bacteria) from environmental water sources. Most well-known bacteriophages infect the common laboratory speciesEscherichia coliandSalmonella enterica, and these phages have built the foundation of molecular and bacteriophage biology. Until now, comparatively few bacteriophages were known to infectShigellaspp., which are close relatives ofE. coli. We present

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Gomez, Mellissa, Alexandra Szewczyk, Jake Szamosi, Vincent Leung, Carlos Filipe, and Zeinab Hosseinidoust. "Stress Exposure of Evolved Bacteriophages under Laboratory versus Food Processing Conditions Highlights Challenges in Translatability." Viruses 15, no. 1 (2022): 113. http://dx.doi.org/10.3390/v15010113.

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Bacterial viruses, or bacteriophages, are highly potent, target-specific antimicrobials. Bacteriophages can be safely applied along the food production chain to aid control of foodborne pathogens. However, bacteriophages are often sensitive to the environments encountered in food matrices and under processing conditions, thus limiting their applicability. We sought to address this challenge by exposing commercially available Listeria monocytogenes bacteriophage, P100, to three stress conditions: desiccation, elevated temperature, and low pH, to select for stress-resistant bacteriophages. The s

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Jamaledin, Rezvan, Rossella Sartorius, Concetta Di Natale, Raffaele Vecchione, Piergiuseppe De Berardinis, and Paolo Antonio Netti. "Recombinant Filamentous Bacteriophages Encapsulated in Biodegradable Polymeric Microparticles for Stimulation of Innate and Adaptive Immune Responses." Microorganisms 8, no. 5 (2020): 650. http://dx.doi.org/10.3390/microorganisms8050650.

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Escherichia coli filamentous bacteriophages (M13, f1, or fd) have attracted tremendous attention from vaccinologists as a promising immunogenic carrier and vaccine delivery vehicle with vast possible applications in the development of vaccines. The use of fd bacteriophage as an antigen delivery system is based on a modification of bacteriophage display technology. In particular, it is designed to express multiple copies of exogenous peptides (or polypeptides) covalently linked to viral capsid proteins. This study for the first time proposes the use of microparticles (MPs) made of poly (lactic-

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Lisa Navitasari, Tri Joko, Rudi Hari Murti, and Triwidodo Arwiyanto. "Aplikasi Actinomycetes dan Bakteriofag pada Tomat Sambung untuk Mengendalikan Penyakit Layu Bakteri Ralstonia solanacearum dan Meningkatkan Hasil Buah." Jurnal Ilmu Pertanian Indonesia 27, no. 4 (2022): 521–27. http://dx.doi.org/10.18343/jipi.27.4.527.

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Ralstonia solanacearum (Smith) is a soil-borne pathogen that causes bacterial wilt disease and is a complex species in races, biovars, phylotypes, and strains of various pathogenicities. As a result, the pathogen is difficult to control. An alternative control is by applying actinomycetes and bacteriophages on tomato grafted with resistant rootstock. This study aims to find the best combination in controlling bacterial wilt disease among grafted tomato plants and/or actinomycetes and/or bacteriophage treatments to increase yields. The graftings were between Amelia (East West, Indonesia) or H79

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Sturino, Joseph M., and Todd R. Klaenhammer. "Expression of Antisense RNA Targeted against Streptococcus thermophilus Bacteriophages." Applied and Environmental Microbiology 68, no. 2 (2002): 588–96. http://dx.doi.org/10.1128/aem.68.2.588-596.2002.

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ABSTRACT Antisense RNA complementary to a putative helicase gene (hel3.1) of a cos-type Streptococcus thermophilus bacteriophage was used to impede the proliferation of a number of cos-type S. thermophilus bacteriophages and one pac-type bacteriophage. The putative helicase gene is a component of the Sfi21-type DNA replication module, which is found in a majority of the S. thermophilus bacteriophages of industrial importance. All bacteriophages that strongly hybridized a 689-bp internal hel3.1 probe were sensitive to the expression of antisense hel3.1 RNA. A 40 to 70% reduction in efficiency o

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Gumbatova, L. A., E. V. Lebedeva, T. A. Sergeeva, B. I. Aslanov, S. A. Gordeeva, and D. A. Gusev. "Possibilities of rational application of bacteriophages in the therapy of infections caused by polyresistant strains of Klebsiella pneumoniae." Journal Infectology 16, no. 4 (2025): 91–98. https://doi.org/10.22625/2072-6732-2024-16-4-91-98.

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The issue of combating antimicrobial resistance has been relevant for several decades, since the arsenal of effective antibiotics used in infectious diseases is significantly reduced, and the development of new antibacterial drugs is significantly difficult and expensive. At the same time, the possibility of widespread use of bacteriophages in hospital settings is being discussed.Goal. Evaluation of the sensitivity to bacteriophages of hospital strains of K.pneumoniae isolated from patients in the departments of surgery and intensive care and intensive care of the Structure Clinical Infectious

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Davydov, D. S., R. L. Parfenyuk, Z. V. Durmanova, and A. A. Movsesyants. "Basic Requirements for the Procedure for Maintaining Collections of Industrial Strains of Bacteriophages." Problems of Particularly Dangerous Infections, no. 4 (December 22, 2024): 146–49. https://doi.org/10.21055/0370-1069-2024-4-146-149.

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The development and implementation of biological drugs based on bacteriophages is one of the elements of the “Strategy for Preventing the Spread of Antimicrobial Resistance in the Russian Federation for the Period of up to 2030”, approved by the Decision of the Government of the Russian Federation, dated September 25, 2017 No. 2045-r. A prerequisite for the implementation of the objectives of the Strategy is the creation of banks of hospital strains of microorganisms and banks of relevant bacteriophages. The aim of the work was to determine the basic requirements for the procedure for maintain

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Connerton, P. L., C. M. Loc Carrillo, C. Swift, et al. "Longitudinal Study of Campylobacter jejuni Bacteriophages and Their Hosts from Broiler Chickens." Applied and Environmental Microbiology 70, no. 7 (2004): 3877–83. http://dx.doi.org/10.1128/aem.70.7.3877-3883.2004.

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ABSTRACT A longitudinal study of bacteriophages and their hosts was carried out at a broiler house that had been identified as having a population of Campylobacter-specific bacteriophages. Cloacal and excreta samples were collected from three successive broiler flocks reared in the same barn. Campylobacter jejuni was isolated from each flock, whereas bacteriophages could be isolated from flocks 1 and 2 but were not isolated from flock 3. The bacteriophages isolated from flocks 1 and 2 were closely related to each other in terms of host range, morphology, genome size, and genetic content. All C

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Farmehr, P. "Phage therapy is an important replacement for the antibiotic resistance." Pakistan Journal of Medical and Health Sciences 15, no. 5 (2021): 1236–40. http://dx.doi.org/10.53350/pjmhs211551236.

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Antibiotic resistance has become a significant and growing threat to public and environmental health. The emergence of multiple drug-resistant bacteria has prompted interest in alternatives to conventional antimicrobial. One of the possible replacement options for antibiotics is the use of bacteriophages as antimicrobial. We were forced to look for a new approach in treatment. Phage therapy is an important alternative antibiotic in the current of drug-resistance pathogens. In this way, poisoning bacteria bacteriophage bacteria infect and replicate in bacteria, in this therapy, identify the typ

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Hervé, C., A. Coste, A. Rouault, J. M. Fraslin, and M. Gautier. "First Evidence of Lysogeny inPropionibacterium freudenreichii subsp.shermanii." Applied and Environmental Microbiology 67, no. 1 (2001): 231–38. http://dx.doi.org/10.1128/aem.67.1.231-238.2001.

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ABSTRACT Dairy propionic acid bacteria, particularly the speciesPropionibacterium freudenreichii, play a major role in the ripening of Swiss type cheese. Isometric and filamentous bacteriophages infecting P. freudenreichii have previously been isolated from cheese. In order to determine the origin of these bacteriophages, lysogeny of P. freudenreichii was determined by isometric bacteriophage type analysis. The genomic DNA of 76 strains were hybridized with the DNA of nine bacteriophages isolated from Swiss type cheeses, and the DNA of 25 strains exhibited strong hybridization. Three of these

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Brentlinger, Karie L., Susan Hafenstein, Christopher R. Novak та ін. "Microviridae, a Family Divided: Isolation, Characterization, and Genome Sequence of φMH2K, a Bacteriophage of the Obligate Intracellular Parasitic Bacterium Bdellovibrio bacteriovorus". Journal of Bacteriology 184, № 4 (2002): 1089–94. http://dx.doi.org/10.1128/jb.184.4.1089-1094.2002.

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ABSTRACT A novel single-stranded DNA phage, φMH2K, of Bdellovibrio bacteriovorus was isolated, characterized, and sequenced. This phage is a member of the Microviridae, a family typified by bacteriophage φX174. Although B. bacteriovorus and Escherichia coli are both classified as proteobacteria, φMH2K is only distantly related to φX174. Instead, φMH2K exhibits an extremely close relationship to the Microviridae of Chlamydia in both genome organization and encoded proteins. Unlike the double-stranded DNA bacteriophages, for which a wide spectrum of diversity has been observed, the single-strand

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Schrader, Holly S., John O. Schrader, Jeremy J. Walker, Thomas A. Wolf, Kenneth W. Nickerson, and Tyler A. Kokjohn. "Bacteriophage infection and multiplication occur inPseudomonas aeruginosastarved for 5 years." Canadian Journal of Microbiology 43, no. 12 (1997): 1157–63. http://dx.doi.org/10.1139/m97-164.

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Bacteriophages specific for Pseudomonas aeruginosa and Escherichia coli were examined for their ability to multiply in stationary phase hosts. Four out of five bacteriophages tested, including E. coli bacteriophage T7M, were able to multiply in stationary phase hosts. The bacteriophage ACQ had a mean burst size of approximately 1000 in exponential phase P. aeruginosa hosts and 102 in starved hosts, with corresponding latent periods that increased from 65 to 210 min. The bacteriophage UT1 had a mean burst size of approximately 211 in exponential phase P. aeruginosa hosts and 11 in starved hosts

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Rapala, Jackson, Brenda Miller, Maximiliano Garcia, et al. "Genomic diversity of bacteriophages infecting Rhodobacter capsulatus and their relatedness to its gene transfer agent RcGTA." PLOS ONE 16, no. 11 (2021): e0255262. http://dx.doi.org/10.1371/journal.pone.0255262.

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The diversity of bacteriophages is likely unparalleled in the biome due to the immense variety of hosts and the multitude of viruses that infect them. Recent efforts have led to description at the genomic level of numerous bacteriophages that infect the Actinobacteria, but relatively little is known about those infecting other prokaryotic phyla, such as the purple non-sulfur photosynthetic α-proteobacterium Rhodobacter capsulatus. This species is a common inhabitant of freshwater ecosystems and has been an important model system for the study of photosynthesis. Additionally, it is notable for

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Stipniece, Liga, Dace Rezevska, Juta Kroica, and Karlis Racenis. "Effect of the Biopolymer Carrier on Staphylococcus aureus Bacteriophage Lytic Activity." Biomolecules 12, no. 12 (2022): 1875. http://dx.doi.org/10.3390/biom12121875.

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The use of implant materials is always associated with the risk of infection. Moreover, the effectiveness of antibiotics is reduced due to antibiotic-resistant pathogens. Thus, selecting the appropriate alternative antimicrobials for local delivery systems is correlated with successful infection management. We evaluated immobilization of the S. aureus specific bacteriophages in clinically recognized biopolymers, i.e., chitosan and alginate, to control the release profile of the antimicrobials. The high-titre S. aureus specific bacteriophages were prepared from commercial bacteriophage cocktail

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