Auswahl der wissenschaftlichen Literatur zum Thema „Cell microscopy“

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Zeitschriftenartikel zum Thema "Cell microscopy"

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Yang, Shuntao. "Digital holographic microscopy of highly sensitive living cells." Journal of Computational Methods in Sciences and Engineering 21, no. 6 (2021): 1985–97. http://dx.doi.org/10.3233/jcm215504.

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In order to solve the problem that the existing living cell microscopy technology can not display the detailed information of cells, a high sensitivity digital holographic living cell microscopy technology is proposed in this paper. By measuring the phase distribution and refractive index distribution of living cells, the data of living cells are extracted and converted into digital hologram of living cells. Simulation and comparison of the commonly used two-dimensional living cell microscope methods. The experimental results show that the high-sensitivity digital holographic microscopic detec
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Wait, Eric C., Michael A. Reiche, and Teng-Leong Chew. "Hypothesis-driven quantitative fluorescence microscopy – the importance of reverse-thinking in experimental design." Journal of Cell Science 133, no. 21 (2020): jcs250027. http://dx.doi.org/10.1242/jcs.250027.

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ABSTRACTOne of the challenges in modern fluorescence microscopy is to reconcile the conventional utilization of microscopes as exploratory instruments with their emerging and rapidly expanding role as a quantitative tools. The contribution of microscopy to observational biology will remain enormous owing to the improvements in acquisition speed, imaging depth, resolution and biocompatibility of modern imaging instruments. However, the use of fluorescence microscopy to facilitate the quantitative measurements necessary to challenge hypotheses is a relatively recent concept, made possible by adv
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Yang, Thomas Zhirui, and Yumin Wu. "Seeing cells without a lens: Compact 3D digital lensless holographic microscopy for wide-field imaging." Theoretical and Natural Science 12, no. 1 (2023): 61–72. http://dx.doi.org/10.54254/2753-8818/12/20230434.

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Optical microscopy is an essential tool for biomedical discoveries and cell diagnosis at micro- to nano-scales. However, conventional microscopes rely on lenses to record 2-D images of samples, which limits in-depth inspection of large volumes of cells. This research project implements a novel 3-D lensless microscopic imaging system that achieves a wide field of view, high resolution, and an extremely compact, cost-effective design: the Digital Lensless Holographic Microscope (DLHM).A lensless holographic microscope is built with only a light source, a sample, and an imaging chip (with other n
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Shotton, D. M. "Video-enhanced light microscopy and its applications in cell biology." Journal of Cell Science 89, no. 2 (1988): 129–50. http://dx.doi.org/10.1242/jcs.89.2.129.

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The combination of novel optical microscopic techniques with advanced video and digital image-processing technology now permits dramatic improvements in the quality of light-microscope images. Such video-enhanced light microscopy has lead to a renaissance in the applications of the light microscope for the study of living cells in two important areas: the intensification of faint fluorescence images, permitting observation of fluorescently labelled cells under conditions of very low illuminating intensity; and the enhancement of extremely low contrast images generated by minute cellular struct
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Volkov, I. A., N. V. Frigo, L. F. Znamenskaya, and O. R. Katunina. "Application of Confocal Laser Scanning Microscopy in Biology and Medicine." Vestnik dermatologii i venerologii 90, no. 1 (2014): 17–24. http://dx.doi.org/10.25208/0042-4609-2014-90-1-17-24.

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Fluorescence confocal laser scanning microscopy and reflectance confocal laser scanning microscopy are up-to-date highend study methods. Confocal microscopy is used in cell biology and medicine. By using confocal microscopy, it is possible to study bioplasts and localization of protein molecules and other compounds relative to cell or tissue structures, and to monitor dynamic cell processes. Confocal microscopes enable layer-by-layer scanning of test items to create demonstrable 3D models. As compared to usual fluorescent microscopes, confocal microscopes are characterized by a higher contrast
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Kosaka, Yudai, and Tetsuhiko Ohba. "3P174 Study on membrane microfluidity of living cells using Muller Matrix microscopy(12. Cell biology,Poster)." Seibutsu Butsuri 53, supplement1-2 (2013): S240. http://dx.doi.org/10.2142/biophys.53.s240_5.

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Schneckenburger, Herbert, and Christoph Cremer. "Axial Tomography in Live Cell Microscopy." Biophysica 4, no. 2 (2024): 142–57. http://dx.doi.org/10.3390/biophysica4020010.

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For many biomedical applications, laser-assisted methods are essential to enhance the three-dimensional (3D) resolution of a light microscope. In this report, we review possibilities to improve the 3D imaging potential by axial tomography. This method allows us to rotate the object in a microscope into the best perspective required for imaging. Furthermore, images recorded under variable angles can be combined to one image with isotropic resolution. After a brief review of the technical state of the art, we show some biomedical applications, and discuss future perspectives for Deep View Micros
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Engels, F. M. "Developments in application of light and scanning electron microscopy techniques for cell wall degradation studies." Netherlands Journal of Agricultural Science 44, no. 4 (1996): 357–73. http://dx.doi.org/10.18174/njas.v44i4.542.

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The results of recent technological developments in light and scanning electron microscopy closely used for research on forage cell wall degradation in ruminants, are reviewed. The indigestibility of forages by rumen microorganisms used to be ascribed mainly to an overall presence of lignin in the plant material. However, early light microscopic observations without application of histochemical staining revealed that some leaf and stem tissues were degraded completely. The early use of lignin detecting dyes, such as acid phloroglucinol or safranin, in light microscopy made it possible to discr
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Prabhakar, Neeraj, Markus Peurla, Olga Shenderova, and Jessica M. Rosenholm. "Fluorescent and Electron-Dense Green Color Emitting Nanodiamonds for Single-Cell Correlative Microscopy." Molecules 25, no. 24 (2020): 5897. http://dx.doi.org/10.3390/molecules25245897.

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Correlative light and electron microscopy (CLEM) is revolutionizing how cell samples are studied. CLEM provides a combination of the molecular and ultrastructural information about a cell. For the execution of CLEM experiments, multimodal fiducial landmarks are applied to precisely overlay light and electron microscopy images. Currently applied fiducials such as quantum dots and organic dye-labeled nanoparticles can be irreversibly quenched by electron beam exposure during electron microscopy. Generally, the sample is therefore investigated with a light microscope first and later with an elect
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Carmichael, Stephen W., and Jon Charlesworth. "Correlating Fluorescence Microscopy with Electron Microscopy." Microscopy Today 12, no. 1 (2004): 3–7. http://dx.doi.org/10.1017/s1551929500051749.

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The use of fluorescent probes is becoming more and more common in cell biology. It would be useful if we were able to correlate a fluorescent structure with an electron microscopic image. The ability to definitively identify a fluorescent organelle would be very valuable. Recently, Ying Ren, Michael Kruhlak, and David Bazett-Jones devised a clever technique to correlate a structure visualized in the light microscope, even a fluorescing cell, with transmission electron microscopy (TEM).Two keys to the technique of Ren et al are the use of grids (as used in the TEM) with widely spaced grid bars
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Dissertationen zum Thema "Cell microscopy"

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Jaritz, Fritz Simon. "Single Cell Expansion Microscopy." Thesis, KTH, Tillämpad fysik, 2020. http://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-279445.

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Raabe, Isabel. "Visualization of cell-to-cell communication by advanced microscopy techniques." Doctoral thesis, Saechsische Landesbibliothek- Staats- und Universitaetsbibliothek Dresden, 2015. http://nbn-resolving.de/urn:nbn:de:bsz:14-qucosa-178404.

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In order to maintain a multicellular organism cells need to interact and communicate with each other. Signalling cascades such as the Bone Morphogenic Protein (BMP) and Hedgehog (Hh) signalling pathways therefore play essential roles in development and disease. Intercellular signalling also underlies the function of stem cell niches, signalling microenvironments that regulate behaviour of associated stem cells. Range and intensity of the niche signal controls stem cell proliferation and differentation and must therefore be strictly regulated. The testis and ovary of the fruit fly Drosophila me
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Ronteix, Gustave. "Inferring cell-cell interactions from quantitative analysis of microscopy images." Thesis, Institut polytechnique de Paris, 2021. http://www.theses.fr/2021IPPAX111.

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Les systèmes biologiques sont bien plus que la somme de leurs constituants. En effet, ils sont souvent caractérisés par des comportements macroscopiques complexes résultant de boucles d'interactions et de rétroactions. Par exemple, la régulation et le rejet éventuel des tumeurs par le système immunitaire est le résultat de multiples réseaux de régulation, influençant à la fois le comportement des cellules cancéreuses et immunitaires. Pour simuler ces effets complexes in-vitro, j'ai conçu une puce microfluidique permettant de confronter des sphéroïdes de mélanome à de multiples cellules T et d'
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Sjögren, Florence. "Dermal cell trafficking : from microscopy to microdialysis /." Linköping : Univ, 2005. http://www.bibl.liu.se/liupubl/disp/disp2005/med883s.pdf.

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Samsuri, Fahmi B. "Single Cell analysis using AtomicForce Microscopy (AFM)." Thesis, University of Canterbury. Electrical and Computer Engineering, 2010. http://hdl.handle.net/10092/5516.

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Replication of biological cells for the purpose of imaging and analysis under electron and scanning probe microscopy has facilitated the opportunity to study and examine some molecular processes and structures of living cells in a manner that were not possible before. The difficulties faced in direct cellular analysis when using and operating Atomic Force Microscopy (AFM) in situ for morphological studies of biological cells have led to the development of a novel method for biological cell studies based on nanoimprint lithography. The realization of the full potential of high resolution AFM im
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Sun, Mingzhai. "Cell mechanics studied using atomic force microscopy." Diss., Columbia, Mo. : University of Missouri-Columbia, 2008. http://hdl.handle.net/10355/5499.

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Thesis (Ph. D.)--University of Missouri-Columbia, 2008.<br>The entire dissertation/thesis text is included in the research.pdf file; the official abstract appears in the short.pdf file (which also appears in the research.pdf); a non-technical general description, or public abstract, appears in the public.pdf file. Title from title screen of research.pdf file (viewed on June 17, 2009) Vita. Includes bibliographical references.
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Nguyen, Tran Thien Dat. "Bayesian Multi-Object Tracking for Cell Microscopy." Thesis, Curtin University, 2021. http://hdl.handle.net/20.500.11937/86947.

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Cell tracking is an essential tool for studying how cells behave and divide under different conditions. This thesis proposes new approaches to track cells and their lineages using random finite set, which allows the tracking errors to be statistically quantified. Additionally, this thesis also explores criteria to rank performance of basic vision task algorithms (e.g., object detection, instance-level segmentation, and tracking), which have not been received proportionate attention from the scientific community.
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López, Ayón Gabriela. "Applying a commercial atomic force microscope for scanning near-field optical microscopy techniques and investigation of Cell-cell signalling." Thesis, McGill University, 2010. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=92400.

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The field of research of this thesis is Condensed Matter Physics applied to Biology. Specifically it describes the development of different Atomic Force Microscopy techniques and tools towards the study of living cells in physiological solution. Particular interest is put into the understanding of the influence of noise in the determination of ordered liquid layers above a mica surface - as work towards the study of the role of water and ions in biological processes - and the influence of "diving bell" to boost the Q factor and allow stable imaging and force spectroscopy with tips based on Sca
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Makarchuk, Stanislaw. "Measurement of cell adhesion forces by holographic microscopy." Thesis, Strasbourg, 2016. http://www.theses.fr/2016STRAE034/document.

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Les forces mécaniques, générées par la cellule jouent un rôle crucial dans l'adhésion cellulaire, qui est un processus commun à un grand nombre de lignées cellulaires. Afin de mesurer la champ des forces pendant l'adhérence cellulaire, nous utilisons la microscopie de force de traction, où la cellule adhère à la surface plane d'un substrat souple dans le plan. Les forces sont calculées à partir du champ de déplacement mesuré à l'intérieur du substrat sous la cellule. Nous avons construit le microscope, dans lequel nous utilisons des billes sphériques en polystyrène pour mesurer le champ de dép
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Magnusson, Klas. "Cell tracking for automated analysis of timelapse microscopy." Thesis, KTH, Signalbehandling, 2011. http://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-53772.

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This document presents an algorithm to automatically perform two dimensional tracking of cells in in-vitro cultures. The developed software handles all the necessary data processing, from preprocessing the images to automaticallytracking the cells and it also provides an interface to manually correct the obtained cell trajectories and functions to analyze the data. The system is developed for, and tested on, muscle stem cells (MuSCs) but it can also be applied to other cell types that look and behave similarly. The software was used in a bio-medical study to investigate the effects on mouse Mu
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Bücher zum Thema "Cell microscopy"

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Brian, Matsumoto, and American Society for Cell Biology., eds. Cell biological applications of confocal microscopy. 2nd ed. Academic Press, 2002.

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Kevin, Foskett J., and Grinstein Sergio, eds. Non-invasivetechniques in cell biology. Wiley-Liss, 1990.

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Kevin, Foskett J., and Grinstein Sergio 1950-, eds. Noninvasive techniques in cell biology. Wiley-Liss, 1990.

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P, Hemmerich, and Diekmann Stephan, eds. Visions of the cell nucleus. American Scientific Publishers, 2005.

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1939-, Plattner Helmut, ed. Electron microscopy of subcellular dynamics. CRC Press, 1989.

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National Institute of Standards and Technology (U.S.), ed. Overlap-based cell tracker. U.S. Dept. of Commerce, National Institute of Standards and Technology, 2009.

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Lansing, Taylor D., Wang Yu-Li, and American Society for Cell Biology., eds. Methods in cell biology.: Imaging and spectroscopy. Academic Press, 1990.

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Masters, Barry R. Confocal microscopy and multiphoton excitation microscopy: The genesis of live cell imaging. SPIE Press, 2006.

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N, Harris, and Oparka K. J, eds. Plant cell biology: Practical approach. IRLPress, 1994.

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1939-, Plattner Helmut, ed. Electron microscopy of subcellular dynamics. CRC Press, 1989.

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Buchteile zum Thema "Cell microscopy"

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O'Farrell, Minnie. "Basic Light Microscopy." In Cell Biology Protocols. John Wiley & Sons, Ltd, 2006. http://dx.doi.org/10.1002/0470033487.ch1.

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Harris, J. Robin, Jeffrey A. Nickerson, and Jean Underwood. "Basic Electron Microscopy." In Cell Biology Protocols. John Wiley & Sons, Ltd, 2006. http://dx.doi.org/10.1002/0470033487.ch2.

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Prins, F. A., I. Cornelese-ten Velde, and E. Heer. "Reflection Contrast Microscopy." In Cell Imaging Techniques. Humana Press, 2006. http://dx.doi.org/10.1007/978-1-59259-993-6_18.

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Gräf, Ralph, Jens Rietdorf, and Timo Zimmermann. "Live Cell Spinning Disk Microscopy." In Microscopy Techniques. Springer Berlin Heidelberg, 2005. http://dx.doi.org/10.1007/b102210.

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Miura, Kota. "Tracking Movement in Cell Biology." In Microscopy Techniques. Springer Berlin Heidelberg, 2005. http://dx.doi.org/10.1007/b102218.

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Carrillo-Barberà, Pau, Jose M. Morante-Redolat, and José F. Pertusa. "Cell Proliferation High-Content Screening on Adherent Cell Cultures." In Computer Optimized Microscopy. Springer New York, 2019. http://dx.doi.org/10.1007/978-1-4939-9686-5_14.

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Gray, Colin, and Daniel Zicha. "Microscopy of Living Cells." In Animal Cell Culture. John Wiley & Sons, Ltd, 2011. http://dx.doi.org/10.1002/9780470669815.ch3.

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Akkaya, Billur, Olena Kamenyeva, Juraj Kabat, and Ryan Kissinger. "Visualizing the Dynamics of T Cell–Dendritic Cell Interactions in Intact Lymph Nodes by Multiphoton Confocal Microscopy." In Confocal Microscopy. Springer US, 2021. http://dx.doi.org/10.1007/978-1-0716-1402-0_13.

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Gierlinger, Notburga. "Raman Imaging of Plant Cell Walls." In Confocal Raman Microscopy. Springer International Publishing, 2018. http://dx.doi.org/10.1007/978-3-319-75380-5_19.

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Gierlinger, Notburga. "Raman Imaging of Plant Cell Walls." In Confocal Raman Microscopy. Springer Berlin Heidelberg, 2010. http://dx.doi.org/10.1007/978-3-642-12522-5_10.

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Konferenzberichte zum Thema "Cell microscopy"

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Peters, Sean, and Ben Ovryn. "Use of a linear feedback loop to null phase variations in a laser feedback microscope." In Novel Techniques in Microscopy. Optica Publishing Group, 2025. https://doi.org/10.1364/ntm.2025.nth1c.5.

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Our laser feedback interference microscope can measure nanometer optical paths in live cells. Implementing a feedback controller, large oscillating phase changes can be measured. We investigate cell transport with Faraday waves in a Hele-Shaw cell.
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Khoubafarin, Somaiyeh, Cyrus Koogan, and Aniruddha Ray. "High-Resolution On-Chip Fluorescence Microscopy for Rapid Screening of Chemotherapeutic Drugs." In Novel Techniques in Microscopy. Optica Publishing Group, 2025. https://doi.org/10.1364/ntm.2025.nw1c.4.

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We developed a cost-effective high-resolution on-chip fluorescence microscope with a wide field-of-view, enabling the imaging of several hundred cells simultaneously. This microscope was used to study drug-induced oxidative stress and cell death in cancer cells.
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Tasmi, Tabassum Ahmad, and Alex J. Walsh. "Propofol Treatment Alters the Metabolic State of MDA-MB-231 Breast Cancer Cells." In Novel Techniques in Microscopy. Optica Publishing Group, 2025. https://doi.org/10.1364/ntm.2025.nw2c.6.

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This study investigates the impact of propofol, an intravenous anesthetic drug, on the metabolic behavior of the breast cancer cell line MDA-MB 231, by utilizing fluorescence lifetime imaging microscopy (FLIM). It revealed significant morphological and metabolic changes in the cells, which may reveal a metabolic-component to propofol’s mechanism.
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Little, Brenda J., Robert K. Pope, Tyrone L. Daulton, and Richard I. Ray. "Application of Environmental Cell Transmission Electron Microscopy to Microbiologically Influenced Corrosion." In CORROSION 2001. NACE International, 2001. https://doi.org/10.5006/c2001-01266.

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Abstract The spatial/chemical relationship between bacteria, their biofilms, and metal substrata was examined in an environmental cell transmission electron microscope equipped with an energy loss spectrometer. The advantage of environmental cell transmission electron microscopy is that unfixed, hydrated specimens can be examined, in more or less their natural state, with high spatial resolution.
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Datta, Rupsa, Emmanuel Contreras Guzman, Kiera M. Sapp, Matthew Vander Heiden, and Melissa C. Skala. "Metabolic imaging of cell division." In Multiphoton Microscopy in the Biomedical Sciences XXV, edited by Ammasi Periasamy, Peter T. So, and Karsten König. SPIE, 2025. https://doi.org/10.1117/12.3043455.

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Zheng, Guoan. "Blood-Cell ‘Lens’ for High-Resolution, High-Throughput Ptychographic Microscopy on a Chip." In Microscopy Histopathology and Analytics. Optica Publishing Group, 2024. https://doi.org/10.1364/microscopy.2024.mtu3a.1.

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This talk presents the use of blood cells as computational lenses for high-resolution, high-throughput coded ptychographic microscopy, and explores its extensions including optofluidic ptychography, synthetic aperture ptychography, depth-multiplexed imaging, among others. The combination of high phase sensitivity, high spatiotemporal resolution, intrinsic molecular contrast, and ultra-large field of view is unique among existing microscopy techniques. Full-text article not available; see video presentation
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Senju, Yosuke. "Three-dimensional ultrastructural analysis of cell-cell junctions in epithelial cells by using super-resolution fluorescence and electron microscopy." In European Microscopy Congress 2020. Royal Microscopical Society, 2021. http://dx.doi.org/10.22443/rms.emc2020.1457.

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Alloyeau, Damien. "Monitoring the dynamic of cell-derived and synthetic vesicles by liquid-cell TEM." In European Microscopy Congress 2020. Royal Microscopical Society, 2021. http://dx.doi.org/10.22443/rms.emc2020.1157.

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Galvez, Dominique, Ricky Cordova, Kelli Kiekens, et al. "Cell-acquiring fallopian endoscope for detection of ovarian cancer via reflectance imaging, fluorescence imaging, and cell collection." In Endoscopic Microscopy XVIII, edited by Melissa J. Suter, Guillermo J. Tearney, and Thomas D. Wang. SPIE, 2023. http://dx.doi.org/10.1117/12.2650875.

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Schonbrun, Ethan, Giuseppe Di Caprio, and Diane Schaak. "Dye Exclusion Cell Microscopy." In Imaging Systems and Applications. OSA, 2013. http://dx.doi.org/10.1364/isa.2013.im3e.3.

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Berichte der Organisationen zum Thema "Cell microscopy"

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Lapeira, Javier. Breast Cancer Endothelial Cell Calcium Dynamics Using Two-Photon Microscopy. Defense Technical Information Center, 2013. http://dx.doi.org/10.21236/ada579069.

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Lapeira, Javier. Breast Cancer Endothelial Cell Calcium Dynamics Using Two-Photon Microscopy. Defense Technical Information Center, 2012. http://dx.doi.org/10.21236/ada558870.

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Nguy, Amanda. Investigating the use of in situ liquid cell scanning transmission electron microscopy. Office of Scientific and Technical Information (OSTI), 2016. http://dx.doi.org/10.2172/1342540.

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Yang, Changhuei. Integrated Device for Circulating Tumor Cell Capture, Characterization, and Lens-Free Microscopy. Defense Technical Information Center, 2012. http://dx.doi.org/10.21236/ada567187.

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Cote, Richard, and Changhuei Yang. Integrated Device for Circulating Tumor Cell Capture, Characterization and Lens-Free Microscopy. Defense Technical Information Center, 2011. http://dx.doi.org/10.21236/ada574570.

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Cote, Richard, Changhuei Yang, and Ram Datar. Integrated Device for Circulating Tumor Cell Capture, Characterization and Lens-Free Microscopy. Defense Technical Information Center, 2012. http://dx.doi.org/10.21236/ada581028.

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Yang, Changhuei. Integrated Device for Circulating Tumor Cell Capture, Characterization, and Lens-Free Microscopy. Defense Technical Information Center, 2011. http://dx.doi.org/10.21236/ada550879.

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Heo, Jaeyoung, Bruce McNamara, and Edgar Buck. In-Situ Liquid Cell Transmission Electron Microscopy of Nanoparticles from Spent Nuclear Fuel. Office of Scientific and Technical Information (OSTI), 2022. http://dx.doi.org/10.2172/1908677.

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Zhang, Yun. Real time imaging of live cell ATP leaking or release events by chemiluminescence microscopy. Office of Scientific and Technical Information (OSTI), 2008. http://dx.doi.org/10.2172/964390.

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Jalali, Bahram, and Dino Di Carlo. Massively Parallel Rogue Cell Detection Using Serial Time-Encoded Amplified Microscopy of Inertially Ordered Cells in High Throughput Flow. Defense Technical Information Center, 2011. http://dx.doi.org/10.21236/ada566873.

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