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1

Adeoba, Mariam I., Ronny Kabongo, Herman Van der Bank, and Kowiyou Yessoufou. "Re-evaluation of the discriminatory power of DNA barcoding on some specimens of African Cyprinidae (subfamilies Cyprininae and Danioninae)." ZooKeys 740 (March 1, 2018): 97–113. http://dx.doi.org/10.3897/zookeys.740.13502.

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Specimen identification in the absence of diagnostic morphological characters (e.g., larvae) can be problematic even for experts. The goal of the present study was to assess the performance of COI in discriminating specimens of the fish family Cyprinidae in Africa, and to explore whether COI-phylogeny can be reliably used for phylogenetic comparative analysis. The main objective was to analyse a matrix of COI sequences for 315 specimens from 15 genera of African Cyprinidae using various distance-based identification methods alongside multiple tests of DNA barcode efficacy (barcode gap, species
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2

Adeoba, Mariam I., Ronny Kabongo, Herman Van der Bank, and Kowiyou Yessoufou. "Re-evaluation of the discriminatory power of DNA barcoding on some specimens of African Cyprinidae (subfamilies Cyprininae and Danioninae)." ZooKeys 744 (March 19, 2018): 67–84. http://dx.doi.org/10.3897/zookeys.744.13502.

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Specimen identification in the absence of diagnostic morphological characters (e.g., larvae) can be problematic even for experts. The goal of the present study was to assess the performance of COI in discriminating specimens of the fish family Cyprinidae in Africa, and to explore whether COI-phylogeny can be reliably used for comparative phylogenetic analysis. The main objective was to analyse a matrix of COI sequences for 315 specimens from 15 genera of African Cyprinidae using various distance-based identification methods alongside multiple tests of DNA barcode efficacy (barcode gap, species
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3

Adeoba, Mariam I., Ronny Kabongo, Herman Van der Bank, and Kowiyou Yessoufou. "Re-evaluation of the discriminatory power of DNA barcoding on some specimens of African Cyprinidae (subfamilies Cyprininae and Danioninae)." ZooKeys 746 (March 26, 2018): 105–21. http://dx.doi.org/10.3897/zookeys.746.13502.

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Specimen identification in the absence of diagnostic morphological characters (e.g., larvae) can be problematic even for experts. The goal of the present study was to assess the performance of COI in discriminating specimens of the fish family Cyprinidae in Africa, and to explore whether COI-phylogeny can be reliably used for phylogenetic comparative analysis. The main objective was to analyse a matrix of COI sequences for 315 specimens from 15 genera of African Cyprinidae using various distance-based identification methods alongside multiple tests of DNA barcode efficacy (barcode gap, species
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4

Adeoba, Mariam I., Ronny Kabongo, der Bank Herman Van, and Kowiyou Yessoufou. "Re-evaluation of the discriminatory power of DNA barcoding on some specimens of African Cyprinidae (subfamilies Cyprininae and Danioninae)." ZooKeys 746 (March 26, 2018): 105–21. https://doi.org/10.3897/zookeys.746.13502.

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Specimen identification in the absence of diagnostic morphological characters (e.g., larvae) can be problematic even for experts. The goal of the present study was to assess the performance of COI in discriminating specimens of the fish family Cyprinidae in Africa, and to explore whether COI-phylogeny can be reliably used for phylogenetic comparative analysis. The main objective was to analyse a matrix of COI sequences for 315 specimens from 15 genera of African Cyprinidae using various distance-based identification methods alongside multiple tests of DNA barcode efficacy (barcode gap, species
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5

Stoffels, Bart E.M.W., der Meij Sancia E.T. van, Bert W. Hoeksema, et al. "Phylogenetic relationships within the Phyllidiidae (Opisthobranchia, Nudibranchia)." ZooKeys 605 (July 14, 2016): 1–35. https://doi.org/10.3897/zookeys.605.7136.

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The Phyllidiidae (Gastropoda, Heterobranchia, Nudibranchia) is a family of colourful nudibranchs found on Indo-Pacific coral reefs. Despite the abundant and widespread occurrence of many species, their phylogenetic relationships are not well known. The present study is the first contribution to fill the gap in our knowledge on their phylogeny by combining morphological and molecular data. For that purpose 99 specimens belonging to 16 species were collected at two localities in Indonesia. They were photographed and used to make a phylogeny reconstruction based on newly obtained cytochrome oxida
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6

Wulandari, Anisa, V. M. Ani Nurgiartiningsih, Kuswati Kuswati, Tri Eko Susilorini, and Paskah Partogi Agung. "Filogeni Beberapa Sapi Lokal Indonesia Menggunakan DNA Mitokondria COI (Cytochrome Oxidase Sub unit I)." Jurnal Ilmu dan Teknologi Peternakan Tropis 6, no. 2 (2019): 278. http://dx.doi.org/10.33772/jitro.v6i2.6165.

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ABSTRAKTujuan penelitian ini adalah untuk mendapatkan konstruksi pohon filogeni berdasarkan keragaman DNA Mitokondria khususnya COI (Cytochrome Oxidase Sub unit I). Sampel yang digunakan yakni darah sapi PO, DNA sapi SO, sapi pesisir, sapi bali, sapi madura dan sapi pasundan masing-masing 12 sampel. Sekuen COI dianalisis menggunakan MEGA 7.0 software dengan metode bootstrapped Neighboor-Joining (NJ) 1000 kali pengulangan berdasarkan between group untuk merekonstruksi pohon filogeni. Hasil penelitian menunjukkan sapi pesisir dekat dengan sapi SO, sapi madura dan sapi pasundan tidak memiliki kek
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7

Bhat, Sunaullah, A. R. N. S. Subbanna, Amit Paschapur, J. Stanley, Sandeep Kumar, and Jai Prakash Gupta. "Phylogeny of Indian Himalayan population of Bombus haemorrhoidalis Smith 1852 (Hymenoptera: Apidae) inferred from mitochondrial DNA sequences." ENTOMON 47, no. 1 (2022): 61–70. http://dx.doi.org/10.33307/entomon.v47i1.689.

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Molecular variation and phylogenetic relationship of Bombus haemorrhoidalis Smith 1852 was studied using partial cytochrome oxidase I (COI) and cytochrome b (cyt b) sequences of mitochondrial genome. The COI and cyt b sequences were compared with available sequences of B. haemorrhoidalis and other Bombus species belonging to different subgenera to avail divergence within and between species. The BLASTn analysis of obtained COI sequence had cent percent identity to a hymenopteran species BOLD deposit AAC6447 (MAHYM005-10.COI-5P) which is a Bombus species from Pakistan. Both these species formed
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8

Bose, C. Nitha, Anu Boswell, and Francy K. Kakkassery. "A molecular phylogeny of Zygopterans (Insecta, Odonata) of Kerala, India." Journal of Insect Biodiversity and Systematics 11, no. 1 (2025): 195–205. https://doi.org/10.61186/jibs.11.1.195.

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Molecular phylogenetic reconstruction of the suborder Zygoptera based on sequences of the nuclear ribosomal gene 18S and mitochondrial gene COI was carried out using species collected from India. Sequence samples of 19 species belonging to 7 families of Zygoptera were used for the analysis. All the existing family levels in Zygoptera were confirmed as monophyletic clades in both analyses. While the 18S analysis resolved deep relations well, the COI analyses supported recently diverged clades. The analysis based on the COI gene showed the monophyly of families Coenagrionidae, Calopterygidae, Le
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Khan, Haseeb Ahmad, and Ibrahim Abdulwahid Arif. "COI barcodes and phylogeny of doves (Columbidae family)." Mitochondrial DNA 24, no. 6 (2013): 689–96. http://dx.doi.org/10.3109/19401736.2013.773319.

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10

Kim, Gyeongmin, and Ui Wook Hwang. "The population genetic structure and phylogeographic dispersal of Nodularia breviconcha in the Korean Peninsula based on COI and 16S rRNA genes." PLOS ONE 18, no. 7 (2023): e0288518. http://dx.doi.org/10.1371/journal.pone.0288518.

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A freshwater mussel, Nodularia breviconcha (Mollusca: Bivalvia: Unionida) is endemic to Korean Peninsula. It has recently been taxonomically reexamined and elevated from a subspecies of N. douglasiae to an independent species. But population genetic studies for the species have rarely been conducted. To explore the population genetic structure of N. breviconcha, the nucleotide sequences of cytochrome oxidase subunit I(COI) and 16S rRNA genes from 135 N. breviconcha individuals, including 52 from this study and 83 from Choi et al. (2020). We found 23 COI and 11 16S rRNA genes haplotypes. Phylog
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Noreen, Tuba, Tanveer Hussain, Muhammad Mansha, et al. "Molekularna filogenija i karakterizacija mundri ovce (Ovis aries) u Pakistanu sekvenciranjem mitohondrijskog citokroma b i podjedinice I citokrom oksidaze." Veterinarska stanica 54, no. 6 (2023): 637–92. http://dx.doi.org/10.46419/vs.54.6.4.

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The main focus of this research is to determine the molecular phylogeny and characterization of Mundri Sheep (Ovis aries) through sequencing of mitochondrial Cytochrome b and Cytochrome Oxidase Subunit I (COI). This sheep breed appears morphologically different from other local sheep breeds of Pakistan. The current research is carried out to appraise the status of Mundri sheep whether it is aa different breed from other breeds or not. Blood samples of Mundri sheep were collected from Livestock Experiment Station, (LES) Fazilpur in district Rajanpur (Punjab). DNA was isolated and subjected to P
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12

Liu, Mingxin, Christopher P. Burridge, Laurence J. Clarke, Susan C. Baker, and Gregory J. Jordan. "Does phylogeny explain bias in quantitative DNA metabarcoding?" Metabarcoding and Metagenomics 7 (June 13, 2023): e101266. https://doi.org/10.3897/mbmg.7.101266.

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Estimating species biomass or abundance from the number of high-throughput sequencing (HTS) reads is an aspirational goal for DNA metabarcoding, yet studies have found varied correlations. Performance varies depending on the gene marker and taxonomic group and, in part, may be related to primer-template mismatches, which are likely to exhibit phylogenetic signals. In this study, we compared commonly used fragments of two gene markers for beetles, the mitochondrial cytochrome c oxidase subunit I (COI) and 16S ribosomal RNA (16S), which have similar lengths, but different propensity for primer-t
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13

Ptacek, Margaret B., Shane K. Sarver, Michael J. Childress, and William F. Herrnkind. "Molecular phylogeny of the spiny lobster genus Panulirus (Decapoda: Palinuridae)." Marine and Freshwater Research 52, no. 8 (2001): 1037. http://dx.doi.org/10.1071/mf01070.

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Phylogenetic relationships among all described species and four subspecies (total of 21 taxa) of the spiny lobster genus Panulirus White, 1847, were examined with nucleotide sequence data from portions of two mitochondrial genes, large-subunit ribosomal RNA (16S) and cytochrome oxidase subunit I (COI). Multiple sequence alignments were subjected to maximum-parsimony, neighbour-joining, and maximum-likelihood analysis with Jasus edwardsii as the outgroup. Two major lineages within Panulirus were recovered by all three methods for both the 16S and COI alignments analysed separately and for the c
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14

CASIRAGHI, M., T. J. C. ANDERSON, C. BANDI, C. BAZZOCCHI, and C. GENCHI. "A phylogenetic analysis of filarial nematodes: comparison with the phylogeny of Wolbachia endosymbionts." Parasitology 122, no. 1 (2001): 93–103. http://dx.doi.org/10.1017/s0031182000007149.

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Infection with the endosymbiotic bacteria Wolbachia is widespread in filarial nematodes. Previous studies have suggested concordance between the phylogeny of Wolbachia with that of their nematode hosts. However, there is only one published molecular phylogenetic study of filarial species, based on the 5S rRNA gene spacer. The phylogeny proposed by this study is partially incongruent with previous classifications of filarial nematodes, based on morphological characters. Furthermore, both traditional classifications and molecular phylogenies are, in part, inconsistent with the phylogeny of Wolba
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15

Karpiński, Lech, Patrick Gorring, and Anthony I. Cognato. "DNA vs. Morphology in Delineating Species Boundaries of Endemic Mongolian Eodorcadion Taxa (Coleoptera: Cerambycidae)." Diversity 15, no. 5 (2023): 662. http://dx.doi.org/10.3390/d15050662.

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This paper provides the first DNA sequences and phylogenetic insight into the Central Asian genus Eodorcadion. We used four molecular markers (COI, CAD, ITS2, and Histone 3) and investigated COI genetic distances to verify phylogenetic position of closely related taxa endemic to Mongolia of the ‘Eodorcadion intermedium species-group’. Histone3 data are presented for the first time for Cerambycidae species. We also designed new PCR primers for better amplification of the Cerambycidae COI barcode region. Morphology of all targeted taxa was examined by means of scanning electron microscopy. Our s
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Budak, Mahir, E. Mahir Korkmaz, and Hasan Basibuyuk. "A molecular phylogeny of the Cephinae (Hymenoptera, Cephidae) based on mtDNA COI gene: a test of traditional classification." ZooKeys 130 (September 24, 2011): 363–78. https://doi.org/10.3897/zookeys.130.1466.

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Cephinae is traditionally divided into three tribes and about 24 genera based on morphology and host utilization. There has been no study testing the monophyly of taxa under a strict phylogenetic criterion. A molecular phylogeny of Cephinae based on a total of 68 sequences of mtDNA COI gene, representing seven genera of Cephinae, is reconstructed to test the traditional limits and relationships of taxa. Monophyly of the traditional tribes is not supported. Monophyly of the genera are largely supported except for <i>Pachycephus.</i> A few host shift events are suggested based on phylogenetic re
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17

Gunter, Felicitas, Joh R. Henschel, Mike D. Picker, Jens Oldeland, and Norbert Jürgens. "Phylogeny of sand termites." Biodiversity & Ecology 7 (November 13, 2022): 51–54. http://dx.doi.org/10.7809/b-e.00364.

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The sand termite Psammotermes allocerus is widely distributed in in southern Africa. To investigate the genetic diversity of this species 113 samples were collected in the arid regions of South Africa, Namibia and Angola in fairy circles and also in landscapes without fairy circles. The mitochondrial markers COI and COII were selected to perform Bayesian Inference analysis. The phylogenetic tree is composed of eight well-supported genetic groups within the species complex. In a synopsis with also studied morphological features the eight groups Northern Namib, Western Kalahari Basin, Nama, Sout
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18

Abdulla Agha, Waran N., Hero M. Muhammad, and Kamaran M. Taha. "Phylogenetic analysis among some species of aphids (Homoptera: Aphididae) using DNA sequencing moleculartechnique." Tikrit Journal for Agricultural Sciences 23, no. 3 (2023): 71–78. http://dx.doi.org/10.25130/tjas.23.3.8.

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The phylogeny of aphids (Hemiptera: Aphididae) has been studied by molecular technique for the first time in Kurdistan, this study comprises relationship between six species of Aphids collected from the leaves of different plants (Chrysanthemum, Oak, Almond, Pine, Asteraceae and Herbaceous) in many localities of Erbil governorate Kurdistan region-Iraq, started in May to July 2022. For understanding about aphid evolution, phylogenetic of aphid is crucial. However, neither the phylogenetic alterations of the Aphid taxa nor their comprehensive definition have been achieved. Therefore, a unique me
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Oro, Violeta, Nenad Milovanovic, Violeta Petrovic, Bogdan Nikolic, and Jovana Blagojevic. "Mitochondrial coi in phylogenetic relationships of Laimaphelenchus belgradiensis (nematoda: Aphelenchoididae)." Genetika 47, no. 3 (2015): 909–16. http://dx.doi.org/10.2298/gensr1503909o.

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Nematodes of the genus Laimaphelenchus are small and tiny organisms. Some parts of their body are measured in nanometers. The identification and classification of such organisms is a complex task. Previously, the major source of classification was morphology based on anatomical characters and measurements. Nowadays, this approach is supplemented by: ?nano-morphology? based on scanning electron microscopy and molecular data and phylogeny, resulting in molecular systematics. Laimaphelenchus belgradiensis was recently described species. Since cytochrome c oxidase subunit I gene was successful in
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GUO, WEI-NAN, FANG-RU NAN, ZHONG-SHI HE, et al. "Morphology and molecular phylogeny of Virescentia guangxiensis (Batrachospermaceae, Rhodophyta)." Phytotaxa 687, no. 1 (2025): 84–100. https://doi.org/10.11646/phytotaxa.687.1.6.

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Two collections of freshwater red algae from Zhejiang and Shanxi Province in China are described morphologically and their phylogenetic relationships investigated through molecular sequencing of the rbcL and COI genes. The molecular sequences show a close relationship to Virescentia guangxiensis, a species previously reported from Guangxi and Henan Province, China, particularly through robust support values for rbcL (97/0.95, 70/0.86 ML and BI, respectively) and COI (100/1.00). Morphologically, one sample is a multiseriate gametophyte stage, the other a uniseriate ‘Chantransia’ stage. The game
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Pérez-Sayas, Consuelo, Tatiana Pina, Beatriz Sabater-Muñoz, María Antonia Gómez-Martínez, Josep A. Jaques, and Mónica A. Hurtado-Ruiz. "DNA Barcoding and Phylogeny of Acari Species Based on ITS and COI Markers." Journal of Zoological Systematics and Evolutionary Research 2022 (June 21, 2022): 1–13. http://dx.doi.org/10.1155/2022/5317995.

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Acari harbor numerous minute species of agricultural economic importance, mainly Tetranychidae and Phytoseiidae. Great efforts have been established by means of recovering morphological, molecular, and phylogenetic traits for species identification. Traditional identification still relies on external diagnostic characters, which are limited and usually exhibit large phenotypic plasticity within the species, rendering them useless for species delimitation and identification. We decided to increase the number of sequences of the Acari mitochondrial COI (Cytochrome C oxidase I) marker and ITS nuc
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22

LIN, RUNG-JUEN, YU-CHI LIN, and YU-FENG HSU. "Systematic position of Bombyx incomposita (Lepidoptera: Bombycidae), with notes on its immature biology and hostplant association." Zootaxa 5052, no. 1 (2021): 91–104. http://dx.doi.org/10.11646/zootaxa.5052.1.5.

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The immature stages, morphology, and systematic position of Bombyx incomposita (van Eecke, 1929) are reported. The morphology of larva was investigated using scanning electron microscopy, and the phylogeny was inferred using six genes (COI, CAD, EF-1α, GAPDH, RpS5, and wgl). The molecular phylogeny strongly supported that B. incomposita is sister to B. huttoni Westwood, 1847. The divergence of the COI barcode, phylogenetic reconstruction, and difference in immature stages support the current classification, in which the species incomposita belongs to the genus Bombyx. We give illustrations of
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23

Kalawate, Aparna Sureshchandra, Shital Pawara, A. Shabnam, and K.P. Dinesh. "DNA barcode reveals the occurrence of Palearctic Olepa schleini Witt et al., 2005 (Lepidoptera: Erebidae: Arctiinae) from peninsular India with morphological variations and a new subspecies." Journal of Threatened Taxa 12, no. 9 (2020): 16143–52. http://dx.doi.org/10.11609/jott.5596.12.9.16143-16152.

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The present study was taken up to report a new record of the tiger moth genus, Olepa Watson, 1980 from India along with the discovery of a new subspecies. Earlier the genus was thought to have restricted distribution range in South and South-East Asia until the report of O. schleini Witt, Müller, Kravchenko, Miller, Hausmann &amp; Speidel from the Mediterranean Coastal Plain of Israel in 2005. The species identification and the new subspecies is proposed based on the combination of morphological studies, available literature comparisons, geographical distribution, DNA barcoding and its phyloge
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Chen, Zhong-Guang, Yu-Ting Dai, Xiao-Ping Wu, Jiao Jiang, and Shan Ouyang. "A new species of Petraeomastus Möllendorff, 1901, with an atypical shell morphology from the Lancangjiang River Valley in southwest China (Gastropoda, Stylommatophora, Enidae)." Zoosystematics and Evolution 100, no. 4 (2024): 1201–9. http://dx.doi.org/10.3897/zse.100.130676.

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Our study contains the first molecular phylogeny of Chinese enids based on the mitochondrial markers cytochrome oxidase c (COI) and 16S rRNA (16S). We have sequenced 19 species belonging to 10 out of the 12 currently accepted genera. A new species, Petraeomastus limenghuai Chen, Dai, Wu &amp; Ouyang, sp. nov., is described from the Lancangjiang River Valley in southwest China based on comparative morphology and molecular phylogeny. The currently accepted classification of Chinese enids relies largely on shell morphology and is deemed to require systematic revision.
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Verhoeff, Tristan Joseph. "The molecular phylogeny of cirrate octopods (Cephalopoda: Octopoda: Cirrata) using COI and 16S sequences." Folia Malacologica 31, no. 4 (2023): 175–96. http://dx.doi.org/10.12657/folmal.031.026.

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Contrasting accounts of taxonomic relationships between finned octopod (Octopoda: Cirrata) taxa complicate species identification and generate nomenclatural instability. A comprehensive analysis based on both 16S and COI mitochondrial gene phylogenies is presented, using all currently accepted genera and approximately 27 putative species (including type species for all genera excepting Grimpoteuthis). The goal of this is to stabilise the concepts of genera and families and identify areas needing further systematic research. Four well supported clades are consistent with families Cirroteuthidae
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Chen, Zhong-Guang, Yu-Ting Dai, Xiao-Ping Wu, Jiao Jiang, and Shan Ouyang. "A new species of Petraeomastus Möllendorff, 1901, with an atypical shell morphology from the Lancangjiang River Valley in southwest China (Gastropoda, Stylommatophora, Enidae)." Zoosystematics and Evolution 100, no. (4) (2024): 1201–9. https://doi.org/10.3897/zse.100.130676.

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Our study contains the first molecular phylogeny of Chinese enids based on the mitochondrial markers cytochrome oxidase c (<i>COI</i>) and 16S rRNA (<i>16S</i>). We have sequenced 19 species belonging to 10 out of the 12 currently accepted genera. A new species, <i>Petraeomastus limenghuai</i> Chen, Dai, Wu &amp; Ouyang, sp. nov., is described from the Lancangjiang River Valley in southwest China based on comparative morphology and molecular phylogeny. The currently accepted classification of Chinese enids relies largely on shell morphology and is deemed to require systematic revision.
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27

Karanovic, Tomislav, and Steven J. B. Cooper. "Explosive radiation of the genus Schizopera on a small subterranean island in Western Australia (Copepoda:Harpacticoida): unravelling the cases of cryptic speciation, size differentiation and multiple invasions." Invertebrate Systematics 26, no. 2 (2012): 115. http://dx.doi.org/10.1071/is11027.

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A previously unsurveyed calcrete aquifer in the Yilgarn region of Western Australia revealed an unprecedented diversity of copepods, representing 67% of that previously recorded in this whole region. Especially diverse was the genus Schizopera, with up to four morphospecies per bore and a significant size difference between them. Aims of this study were to: (1) survey the extent of this diversity using morphological and molecular tools; (2) derive a molecular phylogeny based on COI; and (3) investigate whether high diversity is a result of an explosive radiation, repeated colonisations, or bot
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KJER, KARL M., JESSICA A. THOMAS, XIN ZHOU, PAUL B. FRANDSEN, ELIZABETH PRENDINI, and RALPH W. HOLZENTHAL. "Progress on the phylogeny of caddisflies (Trichoptera)." Zoosymposia 10, no. 1 (2016): 248–56. http://dx.doi.org/10.11646/zoosymposia.10.1.23.

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We present our current phylogenetic hypothesis on the phylogeny of Trichoptera, generated from an analysis of over 7000 nucleotides from 18S and 28S rRNA, EF-1α, COI, and CAD. We corroborate our earlier hypotheses, with results that include a monophyletic Annulipalpia, Integripalpia, Brevitentoria, and Plenitentoria. Monophyly of Psychomyioidea, Pseudoneureclipsidae, and Grumichellinae were confirmed. The "Spicipalpian" families were again found to be paraphyletic, and most closely related to Integripalpia. Ptilocolepidae was not found to be monophyletic, but support for its paraphyly was so w
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Bernardo, Umberto, Francesco Nugnes, Simona Gargiulo, et al. "An Integrative Study on Asphondylia spp. (Diptera: Cecidomyiidae), Causing Flower Galls on Lamiaceae, with Description, Phenology, and Associated Fungi of Two New Species." Insects 12, no. 11 (2021): 958. http://dx.doi.org/10.3390/insects12110958.

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An integrative study on some species of Asphondylia was carried out. Two species of gall midges from Italy, Asphondylia rivelloi sp. nov. and Asphondylia micromeriae sp. nov. (Diptera: Cecidomyiidae), causing flower galls respectively on Clinopodium vulgare and Micromeria graeca (Lamiaceae), are described and illustrated. The characteristics of each developmental stage and induced galls are described, which allowed the discrimination of these new species in the complex of Asphondylia developing on Lamiaceae plants. Molecular data based on sequencing both nuclear (ITS2 and 28S-D2) and mitochond
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Pyrcz, Tomasz W., Klaudia Florczyk, Steve Collins, Szabolcs Sáfián, Oscar Mahecha-J, and Dorota Lachowska-Cierlik. "Alpha-taxonomy and phylogeny of African Junoniini butterflies based on morphological data, with an emphasis on genitalia, and COI barcode (Lepidoptera Nymphalidae)." Zootaxa 4991, no. 3 (2021): 401–33. https://doi.org/10.11646/zootaxa.4991.3.1.

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Pyrcz, Tomasz W., Florczyk, Klaudia, Collins, Steve, Sáfián, Szabolcs, Mahecha-J, Oscar, Lachowska-Cierlik, Dorota (2021): Alpha-taxonomy and phylogeny of African Junoniini butterflies based on morphological data, with an emphasis on genitalia, and COI barcode (Lepidoptera Nymphalidae). Zootaxa 4991 (3): 401-433, DOI: 10.11646/zootaxa.4991.3.1
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31

Serri, Sayeh, Johannes Frisch, and Rintelen Thomas von. "Genetic variability of two ecomorphological forms of Stenus Latreille, 1797 in Iran, with notes on the infrageneric classification of the genus (Coleoptera, Staphylinidae, Steninae)." ZooKeys 626 (October 20, 2016): 67–86. https://doi.org/10.3897/zookeys.626.8155.

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In this study, the genetic diversity of Iranian populations of two widespread Stenus species representing two ecomorphological forms, the “open living species” S. erythrocnemus Eppelsheim, 1884 and the “stratobiont” S. callidus Baudi di Selve, 1848, is presented using data from a fragment of the mitochondrial COI gene. We evaluate the mitochondrial cytochrome oxidase I haplotypes and the intraspecific genetic distance of these two species. Our results reveal a very low diversity of COI sequences in S. erythrocnemus in contrast to S. callidus. Moreover, the COI based phylogeny of a selection of
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GROLLMANN, MILENA MARIE, ASLAK JØRGENSEN, and NADJA MØBJERG. "Actinarctus doryphorus (Tanarctidae) DNA barcodes and phylogenetic reinvestigation of Arthrotardigrada with new A. doryphorus and Echiniscoididae sequences." Zootaxa 5284, no. 2 (2023): 351–63. http://dx.doi.org/10.11646/zootaxa.5284.2.7.

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Little is still known about the diversity and evolution of marine arthrotardigrades, as they are generally difficult to sample, resulting in a limited amount of molecular data for barcoding and phylogenetic studies. With the current study, we provide the first investigation into COI haplotype diversity in a marine tanarctid and at the same time readdress arthrotardigrade phylogeny. Specifically, we provide COI mtDNA, 18S and 28S rDNA sequences from a population of Actinarctus doryphorus (Tanarctidae) sampled off the coast of Roscoff, France and further provide new 18S sequences from two marine
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KNEBELSBERGER, THOMAS, and MICHAEL A. MILLER. "Revision and phylogeny of the subaptera-group of Phyllodromica (Blattoptera: Blattellidae: Ectobiinae), including a parthenogenetic species and the evaluation of COI sequences for species identification (DNA barcoding)." Zootaxa 1522, no. 1 (2007): 1–68. https://doi.org/10.11646/zootaxa.1522.1.1.

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KNEBELSBERGER, THOMAS, MILLER, MICHAEL A. (2007): Revision and phylogeny of the subaptera-group of Phyllodromica (Blattoptera: Blattellidae: Ectobiinae), including a parthenogenetic species and the evaluation of COI sequences for species identification (DNA barcoding). Zootaxa 1522 (1): 1-68, DOI: 10.11646/zootaxa.1522.1.1, URL: http://dx.doi.org/10.11646/zootaxa.1522.1.1
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Rawal, Deepak. "Identification and confirmation of Chironomus circumdatus Kieffer, 1916 (Diptera: Chironomidae) using DNA Barcoding." International Journal of Zoological Investigations 7, no. 1 (2021): 301–7. https://doi.org/10.5281/zenodo.7598534.

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Present study was performed to identify and confirm collected larval Chironomid up to species level. To assess molecular taxonomy and phylogeny, DNA barcoding was done using Sanger dideoxy sequencing of mitochondrial cytochrome c oxidase subunit I (COI) gene of larval sample. Bioinformatics analysis was done by using NCBI&rsquo;s BLAST software. By evidence of DNA barcoding, it was confirmed that present larval chironomid species was Chironomus circumdatus Kieffer, 1916.
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Borisova, Polina, and Nataliya Budaeva. "First Molecular Phylogeny of Lumbrineridae (Annelida)." Diversity 14, no. 2 (2022): 83. http://dx.doi.org/10.3390/d14020083.

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Lumbrineridae is a family of marine annelids with simple external morphology but complex and diverse jaw apparatuses consisting of paired maxillae and mandibles. Here we present the first phylogeny of lumbrinerids based on combination of nuclear (18S rDNA) and mitochondrial (COI, 16S rDNA) markers utilizing Bayesian inference and Maximum Likelihood approaches. Despite limited taxon sampling, our results support monophyly of the genera Abyssoninoe, Augeneria, Gallardoneris, Lumbrineriopsis, and Ninoe and indicate polyphyly of the genera Lumbrineris (the type genus of the family) and Scoletoma.
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Dunayev, Evgeny A., Evgeniya N. Solovyeva, and Nikolay A. Poyarkov. "Systematics, Phylogeny, and Evolution of Phrynocephalus (Superspecies przewalskii) (Reptilia: Agamidae)." Russian Journal of Herpetology 28, no. 1 (2021): 43–59. http://dx.doi.org/10.30906/1026-2296-2021-28-1-43-59.

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The superspecies przewalskii group of Phrynocephalus includes several taxa with unclear taxonomic status. We analyze a fragment of the mitochondrial DNA gene COI and the body patterns of 275 specimens including type specimens. The results resolve the taxonomy and phylogeny of the group and we provide a diagnostic key for the species.
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HUANG, SI-YAO, YONG-XIANG HOU, LI-JUAN ZHU, and LIU-SHENG CHEN. "First record of the family Neopseustidae from Yunnan, China with description of a new species (Lepidoptera, Neopseustidae)." Zootaxa 5357, no. 4 (2023): 587–94. http://dx.doi.org/10.11646/zootaxa.5357.4.6.

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The family Neopseustidae is reported from Yunnan Province, Southwest China, for the first time, with a new species, N. gaoligongensis Huang &amp; Chen, sp. n., described from Mt. Gaoligong, West Yunnan, based on molecular and morphological evidence. The new species was found to belong to the N. bicornuta species-group and is closely related to N. fanjingshana Yang, 1988, but can be distinguished from it by the differences in morphology and a 3.2% COI distance. Adults and genitalia of the new species and N. fanjingshana are illustrated, and the molecular phylogeny based on COI barcoding of the
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38

Wiemers, Martin, Nicolas Chazot, Christopher Wheat, Oliver Schweiger, and Niklas Wahlberg. "A complete time-calibrated multi-gene phylogeny of the European butterflies." ZooKeys 938 (June 4, 2020): 97–124. http://dx.doi.org/10.3897/zookeys.938.50878.

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With the aim of supporting ecological analyses in butterflies, the third most species-rich superfamily of Lepidoptera, this paper presents the first time-calibrated phylogeny of all 496 extant butterfly species in Europe, including 18 very localised endemics for which no public DNA sequences had been available previously. It is based on a concatenated alignment of the mitochondrial gene COI and up to eleven nuclear gene fragments, using Bayesian inferences of phylogeny. To avoid analytical biases that could result from our region-focussed sampling, our European tree was grafted upon a global g
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Wiemers, Martin, Nicolas Chazot, Christopher W. Wheat, Oliver Schweiger, and Niklas Wahlberg. "A complete time-calibrated multi-gene phylogeny of the European butterflies." ZooKeys 938 (June 4, 2020): 97–124. https://doi.org/10.3897/zookeys.938.50878.

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With the aim of supporting ecological analyses in butterflies, the third most species-rich superfamily of Lepidoptera, this paper presents the first time-calibrated phylogeny of all 496 extant butterfly species in Europe, including 18 very localised endemics for which no public DNA sequences had been available previously. It is based on a concatenated alignment of the mitochondrial gene COI and up to eleven nuclear gene fragments, using Bayesian inferences of phylogeny. To avoid analytical biases that could result from our region-focussed sampling, our European tree was grafted upon a global g
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40

Drohvalenko, M., A. Mykhailenko, M. Rekrotchuk, et al. "Application Of Dna Barcoding In Taxonomy And Phylogeny: An Individual Case Of Coi Partial Gene Sequencing From Seven Animal Species." Vestnik Zoologii 53, no. 5 (2019): 375–84. https://doi.org/10.2478/vzoo-2019-0034.

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Drohvalenko, M., Mykhailenko, A., Rekrotchuk, M., Shpak, L., Shuba, V., Trokhymchuk, R., Utevsky, S., Zinenko, O. (2019): Application Of Dna Barcoding In Taxonomy And Phylogeny: An Individual Case Of Coi Partial Gene Sequencing From Seven Animal Species. Vestnik Zoologii 53 (5): 375-384, DOI: 10.2478/vzoo-2019-0034, URL: http://dx.doi.org/10.2478/vzoo-2019-0034
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41

Maldaner, Maria E., Fernando Z. Vaz-de-Mello, Daniela M. Takiya, and Daniela C. Ferreira. "Molecular Phylogeny of Coprophanaeus (Megaphanaeus) d’Olsoufieff, 1924 (Coleoptera: Scarabaeidae: Scarabaeinae) and the position of C. bellicosus." Insect Systematics & Evolution 51, no. 2 (2020): 241–55. http://dx.doi.org/10.1163/1876312x-00002198.

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Coprophanaeus (Megaphanaeus) d’Olsoufieff, 1924 has four valid species: C. lancifer (Linné, 1767), C. ensifer (Germar, 1821), C. bonariensis (Gory, 1844) and C. bellicosus (Olivier, 1789). However, authors disagree about the placement of C. bellicosus. Thus, our aims were (I) to test if Megaphanaeus is a monophyletic group and (II) verify to which subgenus C. bellicosus belongs. We sequenced three mitochondrial and one nuclear marker for ten Phanaeini species: COI (672bp), COI-II (1326bp), 16S (527bp) and 28S (994bp). For fifteen species we sequenced two markers, COI (681pb) and 16S (532pb). B
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Damadi, Ehsan, Faezeh Yazdani Moghaddam, and Mehdi Ghanbarifardi. "Species delimitation, molecular phylogeny and historical biogeography of the sweetlips fish (Perciformes, Haemulidae)." Zoosystematics and Evolution 99, no. 1 (2023): 135–47. http://dx.doi.org/10.3897/zse.99.96386.

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The subfamily Plectorhinchinae (sweetlips) is composed of poorly-known species with high commercially and ecologically values that exhibit phenotypic plasticity and various morphologies. Few studies have assessed the validity of sweetlips, intergeneric relationships and evolutionary survey in this subfamily, which have not yet been resolved. This study investigated the DNA sequences of (1) the mitochondrial COI gene to delimit species, and (2) two mitochondrial (COI and Cyt b), and one nuclear (RAG1) markers to infer phylogenetic relationships and evolutionary and biogeographic history. The mo
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Damadi, Ehsan, Moghaddam Faezeh Yazdani, and Mehdi Ghanbarifardi. "Species delimitation, molecular phylogeny and historical biogeography of the sweetlips fish (Perciformes, Haemulidae)." Zoosystematics and Evolution 99, no. (1) (2023): 135–47. https://doi.org/10.3897/zse.99.96386.

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The subfamily Plectorhinchinae (sweetlips) is composed of poorly-known species with high commercially and ecologically values that exhibit phenotypic plasticity and various morphologies. Few studies have assessed the validity of sweetlips, intergeneric relationships and evolutionary survey in this subfamily, which have not yet been resolved. This study investigated the DNA sequences of (1) the mitochondrial COI gene to delimit species, and (2) two mitochondrial (COI and Cyt b), and one nuclear (RAG1) markers to infer phylogenetic relationships and evolutionary and biogeographic history. The mo
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44

Krupitsky, Anatoly, Nazar Shapoval, and Galina Shapoval. "DNA Barcoding of the Palaearctic Elfin Butterflies (Lepidoptera, Lycaenidae) with a Description of Four New Species from Vietnam." Insects 14, no. 4 (2023): 352. http://dx.doi.org/10.3390/insects14040352.

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Phylogenetic analysis is provided for the first time for 12 species of Palaearctic elfin butterflies, members of the previously recognized genera Ahlbergia Bryk, 1947, Cissatsuma Johnson, 1992, and Novosatsuma Johnson, 1992, based on the barcoding region of the mitochondrial cytochrome C oxidase subunit I gene (COI). Comparison of the COI barcodes revealed very low levels of genetic divergence between the species of the Palaearctic elfin butterflies and Callophrys Billberg, 1820 sensu stricto. COI-based phylogeny revealed that Palaearctic Callophrys and the Palaearctic elfin butterflies, excep
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Kjer, Karl, Frank Carle, Jesse Litman, and Jessica Ware. "A molecular phylogeny of Hexapoda." Arthropod Systematics & Phylogeny 64, no. 1 (2006): 35–44. http://dx.doi.org/10.3897/asp.64.e31642.

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We present a supermatrix approach to the phylogeny of Insecta that stemmed from a talk given at the 2nd Dresden Meeting on Insect Phylogeny (2005). The data included a fragment of the 28S (D1–D8) and complete sequences for the 18S, histone (H3), EF-1α, COI, COII, the 12S and 16S plus the intervening tRNA, and 170 morphological characters. Ribosomal RNA sequences were manually aligned to secondary structure. Two separate Bayesian likelihood analyses were performed, as well as a weighted parsimony analysis, on combined data. Partitioned datasets were also explored. Expected clades like Hexapoda,
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46

Kjer, Karl, Frank Carle, Jesse Litman, and Jessica Ware. "A molecular phylogeny of Hexapoda." Arthropod Systematics & Phylogeny 64 (October 30, 2006): 35–44. https://doi.org/10.3897/asp.64.e31642.

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We present a supermatrix approach to the phylogeny of Insecta that stemmed from a talk given at the 2nd Dresden Meeting on Insect Phylogeny (2005). The data included a fragment of the 28S (D1–D8) and complete sequences for the 18S, histone (H3), EF-1α, COI, COII, the 12S and 16S plus the intervening tRNA, and 170 morphological characters. Ribosomal RNA sequences were manually aligned to secondary structure. Two separate Bayesian likelihood analyses were performed, as well as a weighted parsimony analysis, on combined data. Partitioned datasets were also explored. Expected clades like Hexapoda,
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47

Gu, Jun-Jie, Chengjie Zheng, Su-Rong Jiang, and Yanli Yue. "Phylogeny of genus Sichuana Shen & Yin, 2020 (Orthoptera, Tettigoniidae, Tettigoniinae) with four new species from Sichuan, China." Deutsche Entomologische Zeitschrift 70, no. (2) (2023): 337–55. https://doi.org/10.3897/dez.70.105870.

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Four new species of Sichuana Shen &amp; Yin, 2020 are described based on morphological comparison and molecular analysis: S. planicercata sp. nov., S. curvicercata sp. nov., S. longilamina sp. nov. and S. magnicerca sp. nov. Specimens showed some intraspecific variation of male tegmina and subgenital plates. The genes COI and 16S were used to analyze the genetic distance between species and COI was used to analyze the phylogenetic relationship of Sichuana.
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48

Liu, Lili, Liping Zhang, Kaiyu Hou, Liyang Ning, and Ruiwen Wu. "Addition to the known diversity of Chinese freshwater mussels: integrative description of a new species of Postolata Dai et al., 2023 (Bivalvia, Unionidae, Gonideinae)." Zoosystematics and Evolution 100, no. 3 (2024): 769–78. http://dx.doi.org/10.3897/zse.100.126069.

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In this study, we present a new species of freshwater mussel in the genus PostolataDai et al., 2023, from Guangxi Province, China, by integrating morphological, anatomical, and molecular data. Postolata longjiangensis Liu &amp; Wu, sp. nov. is distinguished from its congener (i.e., Postolata guangxiensis) by its shell shape, beak position, surface sculpture, nacre color, and hinge structure. Molecular species delimitation results based on the mitochondrial COI gene support the separation of Postolata longjiangensis Liu &amp; Wu, sp. nov. from its congener. The multi-locus (COI + 16S rRNA + 28S
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49

Subbotin, Sergei A., Fateh Toumi, Ibrahim Halil Elekçioğlu, Lieven Waeyenberge, and Zahra Tanha Maafi. "DNA barcoding, phylogeny and phylogeography of the cyst nematode species of the Avenae group from the genus Heterodera (Tylenchida: Heteroderidae)." Nematology 20, no. 7 (2018): 671–702. http://dx.doi.org/10.1163/15685411-00003170.

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Summary Among the recognised species groups of Heterodera, the Avenae group is one of the largest with a total of 12 species. Ten of them, H. arenaria, H. aucklandica, H. australis, H. avenae, H. filipjevi, H. mani, H. pratensis, H. riparia, H. sturhani and H. ustinovi, are morphologically closely related and represent the H. avenae species complex, and the other two, H. hordecalis and H. latipons, are morphologically more distinct from this complex. In this study we provide comprehensive phylogenetic analyses of several hundred COI and ITS rRNA gene sequences from the Avenae group using Bayes
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MCLAUGHLIN, JULIANNE E., PAUL B. FRANDSEN, WOLFRAM MEY, and STEFFEN U. PAULS. "A Preliminary Phylogeny of Rhyacophilidae with Reference to Fansipangana and the Monophyly of Rhyacophila." Zoosymposia 14, no. 1 (2019): 189–92. http://dx.doi.org/10.11646/zoosymposia.14.1.20.

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The phylogeny of Rhyacophilidae was explored with 28S ribosomal RNA (rRNA) and Cytochrome Oxidase Subunit I (COI) mitochondrial DNA (mtDNA). Eighty one rhyacophilids were included in the analysis. We found that although Rhyacophilidae was recovered as monophyletic, intrafamilial relationships are not well-resolved using this dataset. Bootstrap support was poor for intrageneric relationships and additional data will be required to present a more robust hypothesis. The recovered phylogeny places Fansipangana as the sister taxon of the rest of Rhyacophilidae. We found that Himalopsyche was nested
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