Thematische Bibliographien / Corneal epithelial cells

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Auswahl der wissenschaftlichen Literatur zum Thema „Corneal epithelial cells“

Autor: Grafiati

Veröffentlicht am 4. Juni 2021

Zuletzt aktualisiert am 21. Februar 2023

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Zeitschriftenartikel zum Thema "Corneal epithelial cells"

Langer, Marian G., C. V. Sundarraj, and Nirmala Sundarraj. "Corneal epithelial-specific cell surface antigen recognized by a monoclonal antibody." Development 94, no. 1 (June 1, 1986): 163–72. http://dx.doi.org/10.1242/dev.94.1.163.

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Monoclonal antibodies, specific against cell surface differentiation antigens of human corneal epithelial cells, were developed using epithelial cells resected from human corneas as the immunogens. One of these antibodies reacted specifically with corneal epithelial cells and not with epithelial cells of other tissues when tested by an indirect immunoperoxidase technique. Nonidet P-40 extracts of different subcellular fractions of human corneal epithelial cells were tested for their reactivity against this antibody using an enzyme-linked immunosorbent assay. The results indicated that the anti

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Alarcon, Irania, Lesley Kwan, Chong Yu, David J. Evans, and Suzanne M. J. Fleiszig. "Role of the Corneal Epithelial Basement Membrane in Ocular Defense against Pseudomonas aeruginosa." Infection and Immunity 77, no. 8 (June 8, 2009): 3264–71. http://dx.doi.org/10.1128/iai.00111-09.

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ABSTRACT Pseudomonas aeruginosa can invade corneal epithelial cells and translocates multilayered corneal epithelia in vitro, but it does not penetrate the intact corneal epithelium in vivo. In healthy corneas, the epithelium is separated from the underlying stroma by a basement membrane containing extracellular matrix proteins and pores smaller than bacteria. Here we used in vivo and in vitro models to investigate the potential of the basement membrane to defend against P. aeruginosa. Transmission electron microscopy of infected mouse corneas in vivo showed penetration of the stroma by P. aer

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Hazlett, L. D., and P. Mathieu. "Glycoconjugates on corneal epithelial surface: effect of neuraminidase treatment." Journal of Histochemistry & Cytochemistry 37, no. 8 (August 1989): 1215–24. http://dx.doi.org/10.1177/37.8.2754252.

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The purpose of this study was to develop a procedure to quantitatively examine corneal epithelial apical cell membrane-associated glycoconjugates. Saccharide moieties on young, mature, and aged corneal epithelial cells were detected and localized in corneas of immature and adult mice by using colloidal gold-labeled lectins and transmission electron microscopy (TEM). In general, dense binding to the corneal epithelial apical surface cell membranes with wheat germ agglutinin (WGA) was seen in the adult, whereas the immature cornea bound less WGA-gold. Neuraminidase digestion decreased binding of

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Augustin, Danielle K., Susan R. Heimer, Connie Tam, Wing Y. Li, Jeff M. Le Due, David J. Evans, and Suzanne M. J. Fleiszig. "Role of Defensins in Corneal Epithelial Barrier Function againstPseudomonas aeruginosaTraversal." Infection and Immunity 79, no. 2 (November 29, 2010): 595–605. http://dx.doi.org/10.1128/iai.00854-10.

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ABSTRACTStudies have shown that epithelium-expressed antimicrobial peptides (AMPs), e.g., β-defensins, play a role in clearing bacteria from mouse corneas already infected withPseudomonas aeruginosa. Less is known about the role of AMPs in allowing the cornea to resist infection when healthy. We previously reported that contact lens exposure, a major cause ofP. aeruginosakeratitis, can inhibit the upregulation of human β-defensin 2 (hBD-2) by corneal epithelial cells in response toP. aeruginosaantigensin vitro. Here, we studied the role of AMPs in maintaining the corneal epithelial barrier toP

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Zieske, J. D., and M. Wasson. "Regional variation in distribution of EGF receptor in developing and adult corneal epithelium." Journal of Cell Science 106, no. 1 (September 1, 1993): 145–52. http://dx.doi.org/10.1242/jcs.106.1.145.

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Epidermal growth factor receptor has been localized to the proliferative cell layers in a variety of stratified squamous epithelia. In the current study, the rat cornea was used as an experimental model to determine if epidermal growth factor receptor is concentrated in epithelial stem cells. Epidermal growth factor receptor was localized using immunofluorescence microscopy in adult and neonatal (1-day to 4-week) rat corneas. Antibody binding to epidermal growth factor receptor was present in basal cells across the adult cornea but was more intense in the limbal zone. In rats 1 day to 1 week o

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Park, Mijeong, Alexander Richardson, Elvis Pandzic, Erwin P. Lobo, J. Guy Lyons, and Nick Di Girolamo. "Peripheral (not central) corneal epithelia contribute to the closure of an annular debridement injury." Proceedings of the National Academy of Sciences 116, no. 52 (December 16, 2019): 26633–43. http://dx.doi.org/10.1073/pnas.1912260116.

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Corneal epithelia have limited self-renewal and therefore reparative capacity. They are continuously replaced by transient amplifying cells which spawn from stem cells and migrate from the periphery. Because this view has recently been challenged, our goal was to resolve the conflict by giving mice annular injuries in different locations within the corneolimbal epithelium, then spatiotemporally fate-mapping cell behavior during healing. Under these conditions, elevated proliferation was observed in the periphery but not the center, and wounds predominantly resolved by centripetally migrating l

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Han, Sang Beom, Farah Nur Ilyana Mohd Ibrahim, Yu-Chi Liu, and Jodhbir S. Mehta. "Efficacy of Modified Amnion-Assisted Conjunctival Epithelial Redirection (ACER) for Partial Limbal Stem Cell Deficiency." Medicina 57, no. 4 (April 10, 2021): 369. http://dx.doi.org/10.3390/medicina57040369.

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Background and objectives: the aim of this study was to analyze the efficacy of a modified “amnion-assisted conjunctival epithelial redirection (ACER)” technique for the treatment of partial limbal stem cell deficiency (LSCD). Materials and methods: the medical records of three patients with partial LSCD who underwent corneal surface reconstruction with modified ACER following superficial keratectomy were retrospectively studied. Briefly, in this technique, an inner amniotic membrane (AM) layer was applied on the corneal surface to promote corneal re-epithelialization. The outer AM layer was a

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Ryu, Jin Suk, So Yeon Kim, Mee Kum Kim, and Joo Youn Oh. "Inflammation Confers Healing Advantage to Corneal Epithelium following Subsequent Injury." International Journal of Molecular Sciences 24, no. 4 (February 7, 2023): 3329. http://dx.doi.org/10.3390/ijms24043329.

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Recent evidence shows that epithelial stem/progenitor cells in barrier tissues such as the skin, airways and intestines retain a memory of previous injuries, which enables tissues to accelerate barrier restoration after subsequent injuries. The corneal epithelium, the outermost layer of the cornea, is the frontline barrier for the eye and is maintained by epithelial stem/progenitor cells in the limbus. Herein, we provide evidence that inflammatory memory also exists in the cornea. In mice, eyes that had been exposed to corneal epithelial injury exhibited faster re-epithelialization of the corn

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Schermer, A., S. Galvin, and T. T. Sun. "Differentiation-related expression of a major 64K corneal keratin in vivo and in culture suggests limbal location of corneal epithelial stem cells." Journal of Cell Biology 103, no. 1 (July 1, 1986): 49–62. http://dx.doi.org/10.1083/jcb.103.1.49.

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In this paper we present keratin expression data that lend strong support to a model of corneal epithelial maturation in which the stem cells are located in the limbus, the transitional zone between cornea and conjunctiva. Using a new monoclonal antibody, AE5, which is highly specific for a 64,000-mol-wt corneal keratin, designated RK3, we demonstrate that this keratin is localized in all cell layers of rabbit corneal epithelium, but only in the suprabasal layers of the limbal epithelium. Analysis of cultured corneal keratinocytes showed that they express sequentially three major keratin pairs

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Bardag-Gorce, Fawzia, Alissa Diaz, Robert Niihara, Jeremy Stark, Daileen Cortez, Alexander Lee, Richard Hoft, and Yutaka Niihara. "Aldehyde Dehydrogenases Expression in Corneal Epithelial Cells with Limbal Stem Cell Deficiency." International Journal of Molecular Sciences 23, no. 7 (April 5, 2022): 4032. http://dx.doi.org/10.3390/ijms23074032.

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Purpose: The purpose of the present study is to investigate the expression of aldehyde dehydrogenases (ALDHs) in rabbit corneas with limbal stem cell deficiency (LSCD) and corneas treated with cultured autologous oral mucosa epithelial cell sheet CAOMECS designed to reconstruct the ocular surface with LSCD. Methods: New Zealand white rabbit autologous oral mucosal epithelial cells were isolated from a buccal biopsy and cultured to be grafted back onto corneas of rabbit model of LSCD. Immunofluorescent staining and Western blot analysis were used to compare the expression of ALDH1A1 and ALDH1A3

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Dissertationen zum Thema "Corneal epithelial cells"

Liu, Ke. "Role of second messengers in controlling growth patterns of corneal epithelial cells /." View thesis, 2002. http://library.uws.edu.au/adt-NUWS/public/adt-NUWS20030718.102224/index.html.

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Thesis (Ph. D.)--University of Western Sydney, 2002.<br>"This thesis is submitted in fulfilment of the requirements of the degree of Doctor of Philosophy to the University of Western Sydney School of Biological Sciences."t.p. Includes bibliographical references (leaves 138-150).

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Binti, Kamarudin Taty Anna. "Differentiation of human pluripotent stem cells into corneal epithelial like cells." Thesis, University of Newcastle upon Tyne, 2018. http://hdl.handle.net/10443/4182.

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Cornea is the clear outermost protective layer of the eye which enables transmission of light onto the retina. The corneal epithelium is regenerated by limbal stem cells (LSCs), whose loss/dysfunction results in limbal stem cell deficiency (LSCD). Transplantations of ex vivo expanded autologous LSCs from patient's healthy eye onto the affected eye have provided a successful treatment for unilateral LSCD. This however is not applicable to patient with total bilateral LSCD, whose both eyes are affected. This thesis investigated the potential of human induced-pluripotent stem cell (hiPSCs) to dif

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Liu, Ke. "Role of second messengers in controlling growth patterns of corneal epithelial cells." Thesis, View thesis, 2002. http://handle.uws.edu.au:8081/1959.7/387.

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The purpose of this thesis was to investigate mechanisms contolling the growth of corneal epithelial cells, particularly the intracellular signals involved with stratification compared with cellular migration and maturation. Buttons of epithelium were cultured in different culture media. The explants were monitored microscopically for their growth patterns and finally fixed and examined for cytokeratin, vimentin and actin. Different growth patterns were observed in the different media, indicating that different signalling patterns must be operating in these cells depending upon the media in wh

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Liu, Ke, University of Western Sydney, of Science Technology and Environment College, and of Science Food and Horticulture School. "Role of second messengers in controlling growth patterns of corneal epithelial cells." THESIS_CSTE_SFH_Liu_K.xml, 2002. http://handle.uws.edu.au:8081/1959.7/387.

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Guo, Ying. "Actin contractility in corneal epithelial cells and regulation of the barrier integrity." [Bloomington, Ind.] : Indiana University, 2006. http://gateway.proquest.com/openurl?url_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:dissertation&res_dat=xri:pqdiss&rft_dat=xri:pqdiss:3229600.

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Thesis (Ph.D.)--Indiana University, School of Optometry, 2006.<br>"Title from dissertation home page (viewed July 11, 2007)." Source: Dissertation Abstracts International, Volume: 67-08, Section: B, page: 4360. Adviser: Sangly Srinivas.

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Terry, S. J. "Identification of regulators and effectors of RhoGTPase signalling in corneal epithelial cells." Thesis, University College London (University of London), 2011. http://discovery.ucl.ac.uk/1306879/.

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Epithelial cells adhere to each other and are connected via a series of junctions. Tight junctions (TJs) are a specific type of junction consisting of heteromeric protein complexes that are linked to the actin cytoskeleton and are important in regulating paracellular permeability and cell polarity. RhoGTPases are small molecular switch proteins that are important regulators of the cytoskeleton and modulators of gene expression. RhoGTPases have thus been identified as being major signalling components associated with TJs. However little is known about how RhoGTPases are regulated to control jun

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Bhattacharya, Pradipta. "The corneal epithelium in health and disease." Thesis, Queensland University of Technology, 2022.

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The cornea is the anterior transparent layer of the eye, and understanding corneal epithelial morphology is valuable in monitoring ocular surface diseases. A meta-analysis showed that central corneal basal cell density and nerve parameters were reduced in diseases affecting the ocular surface including limbal stem cell deficiency. Using newly developed image analysis techniques it was observed that epithelial cell density was highest at the corneal nerve whorl region. A decrease in epithelial cell density was observed in eyes with conjunctival ultraviolet autofluorescence, i.e. with sunlight-i

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Barnard, Zeke. "Analyses of Cytokeratins and p63 Isoforms Expressed by Human Limbal Epithelial Cells." Thesis, Griffith University, 2005. http://hdl.handle.net/10072/367809.

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Cultivated autologous limbal epithelial grafts have shown promise as a valuable treatment to treat ocular surface injuries. The rationale for this therapy is that the limbus contains progenitor cells of the corneal epithelium and that when expanded and transplanted these cells help to restore a healthy epithelium. Nevertheless, this approach is still experimental and the optimal procedure for producing, grafting and assessing the presence of limbal stem cells in these grafts remains under development. The first results chapter examines the differences in cytokeratin expression between dono

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Shortt, A. J. "The limbal epithelial stem cell niche and its relevance to ex-vivo culture and transplantation of corneal limbal epithelial stem cells." Thesis, University College London (University of London), 2009. http://discovery.ucl.ac.uk/18929/.

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Transplantation of ex-vivo cultured limbal epithelial cells (LEC) is an established treatment for total limbal stem cell deficiency. However, this therapy has preceded the scientific understanding of limbal epithelial stem cells (LESC), the LESC niche and the biological mechanism that underpins it. This body of work tested the hypothesis that the LESC niche has a specialised structure and that an understanding of this may lead to improvements in outcomes and understanding of this therapy. The corneal limbus was investigated using 3D confocal microscopy, scanning electron microscopy, wholemount

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Yang, Juan. "Universal corneal epithelial-like cells derived from human embryonic stem cells in a defined, xeno-free, and albumin-free condition for cellularization of a corneal scaffold." Thesis, University of Macau, 2018. http://umaclib3.umac.mo/record=b3953938.

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Bücher zum Thema "Corneal epithelial cells"

Liminga, Maria. Arachidonic Acid Metabolism in Corneal Epithelial Cells: Indentification of Lipoxygenase Isoforms (Comprehensive Summaries of Uppsala Dissertations, 222). Uppsala Universitet, 2000.

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Buchteile zum Thema "Corneal epithelial cells"

Di Girolamo, Nick. "Adult Human Corneal Epithelial Stem Cells." In Adult Stem Cells, 163–97. New York, NY: Springer New York, 2013. http://dx.doi.org/10.1007/978-1-4614-9569-7_7.

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Ali, Haifa, Charles Osei-Bempong, Ani Ray-Chaudhuri, Bakiah Shaharuddin, Arianna Bianchi, Mohit Parekh, and Sajjad Ahmad. "Controversies in Corneal Epithelial Stem Cell Biology." In Adult and Embryonic Stem Cells, 103–17. Totowa, NJ: Humana Press, 2012. http://dx.doi.org/10.1007/978-1-61779-630-2_9.

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Stapleton, Fiona, Jai-Min Kim, Jason Kasses, and Mark D. P. Willcox. "Mechanisms of Apoptosis in Human Corneal Epithelial Cells." In Advances in Experimental Medicine and Biology, 827–34. Boston, MA: Springer US, 2002. http://dx.doi.org/10.1007/978-1-4615-0717-8_117.

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Kolli, Sai, Majlinda Lako, Francisco Figueiredo, and Sajjad Ahmad. "Corneal Epithelial Stem Cells and Their Therapeutic Application." In Trends in Stem Cell Biology and Technology, 319–65. Totowa, NJ: Humana Press, 2009. http://dx.doi.org/10.1007/978-1-60327-905-5_18.

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Crouch, Rosalie, Zhu Ling, and Betty J. Hayden. "Corneal Oxygen Scavenging Systems: Lysis of Corneal Epithelial Cells by Superoxide Anions." In Oxygen Radicals in Biology and Medicine, 1043–46. Boston, MA: Springer US, 1988. http://dx.doi.org/10.1007/978-1-4684-5568-7_172.

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Figueiredo, Gustavo S., Hardeep Singh Mudhar, Majlinda Lako, and Francisco C. Figueiredo. "Corneal Epithelial Stem Cells: Methods for Ex Vivo Expansion." In Essentials in Ophthalmology, 77–97. Cham: Springer International Publishing, 2019. http://dx.doi.org/10.1007/978-3-030-01304-2_6.

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Srinivas, S. P., R. Mutharasan, and S. Fleiszig. "Shear-Induced ATP Release by Cultured Rabbit Corneal Epithelial Cells." In Advances in Experimental Medicine and Biology, 677–85. Boston, MA: Springer US, 2002. http://dx.doi.org/10.1007/978-1-4615-0717-8_95.

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Palmero, Mercedes, Alfonso Blanco, Juan L. Bellot, Nuria Alcoriza, Irene Perez, and Alfredo Orts. "Ultraviolet Light-Induced Damage in Rabbit Corneal Epithelial Cells in Vitro." In Advances in Ocular Toxicology, 63–72. Boston, MA: Springer US, 1997. http://dx.doi.org/10.1007/978-1-4615-5937-5_7.

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Katikireddy, Kishore Reddy, and Ula V. Jurkunas. "Limbal Stromal Tissue Specific Stem Cells and Their Differentiation Potential to Corneal Epithelial Cells." In Embryonic Stem Cell Protocols, 437–44. New York, NY: Springer New York, 2015. http://dx.doi.org/10.1007/7651_2015_229.

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Igarashi, Tsutomu, Koichi Miyake, Noriko Suzuki, Hiroshi Takahashi, and Takashi Shimada. "In Vivo Gene Transfer Into Corneal Epithelial Progenitor Cells By Viral Vectors." In Advances in Experimental Medicine and Biology, 1309–14. Boston, MA: Springer US, 2002. http://dx.doi.org/10.1007/978-1-4615-0717-8_190.

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Konferenzberichte zum Thema "Corneal epithelial cells"

Molladavoodi, Sara, John B. Medley, Maud Gorbet, and H. J. Kwon. "Mechanotransduction in Corneal Epithelial Cells." In ASME 2013 International Mechanical Engineering Congress and Exposition. American Society of Mechanical Engineers, 2013. http://dx.doi.org/10.1115/imece2013-65406.

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Mechanical properties of the cornea can be affected by diseases such as keratoconus. In keratoconus, a decrease in both thickness and rigidity of the cornea is observed. It is currently not clear whether and how changes in mechanical properties of the cornea are associated with corneal epithelial cell behavior. In the present study, polyacrylamide (PAA) gels with different elastic moduli have been prepared and human corneal epithelial cells (HCECs) have been cultured on them. To investigate the effect that changes in elastic modulus may have on adhesion and migration of corneal epithelial cell

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Bandekar, N., A. Wong, D. Clausi, and M. Gorbet. "A novel approach to automated cell counting for studying human corneal epithelial cells." In 2011 33rd Annual International Conference of the IEEE Engineering in Medicine and Biology Society. IEEE, 2011. http://dx.doi.org/10.1109/iembs.2011.6091482.

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Akiba, M., A. Kubota, C. Reisman, Y. Fukuma, K. Nishida, and K. P. Chan. "Evaluation of cultured corneal epithelial cells and epithelial thickness by full-field optical coherence tomography." In Biomedical Optics. Washington, D.C.: OSA, 2008. http://dx.doi.org/10.1364/biomed.2008.bmd71.

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Nam, Eunryel, and Shoji Takeuchi. "Volatile odorant detection by corneal epithelial cells using a perfusable fluidic chamber." In 2017 IEEE 30th International Conference on Micro Electro Mechanical Systems (MEMS). IEEE, 2017. http://dx.doi.org/10.1109/memsys.2017.7863429.

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Fernández-Ferreiro, A., M. González-Barcia, L. Garcia-Quintanilla, A. Luaces, V. Díaz-Tome, I. Alonso-Rodriguez, X. Garcia-Otero, M. Lamas, and FJ Otero-Espinar. "PP-016 Effect of tacrolimus eye drops on human primary corneal epithelial cells." In 22nd EAHP Congress 22–24 March 2017 Cannes, France. British Medical Journal Publishing Group, 2017. http://dx.doi.org/10.1136/ejhpharm-2017-000640.463.

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Boadi, J., V. Sangwal, S. MacNeil, and S. J. Matcher. "System for tracking transplanted limbal epithelial stem cells in the treatment of corneal stem cell deficiency." In SPIE BiOS, edited by Daniel L. Farkas, Dan V. Nicolau, and Robert C. Leif. SPIE, 2015. http://dx.doi.org/10.1117/12.2077921.

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Boadi, Joseph, Stephen J. Matcher, Sheila MacNeil, and Virender S. Sangwan. "System for tracking transplanted limbal epithelial stem cells in the treatment of corneal stem cell deficiency (Conference Presentation)." In Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues IX, edited by Daniel L. Farkas, Dan V. Nicolau, and Robert C. Leif. SPIE, 2016. http://dx.doi.org/10.1117/12.2211308.

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Cobb, Jessica A., Alison C. Dunn, Jiwoon Kwan, Malisa Sarntinoranont, Gregory Sawyer, and Roger Tran-Son-Tay. "A Novel Method for Frictional Testing on Adherent Cells." In ASME 2007 Summer Bioengineering Conference. American Society of Mechanical Engineers, 2007. http://dx.doi.org/10.1115/sbc2007-176552.

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Understanding the physical interaction between biomaterials and tissues within the body is critical for the development of medical devices. For instance, the coefficient of friction between the two must be low enough to avoid damaging the cells that make up the tissue. Therefore, a method for obtaining friction data on cells is needed. In the present work, human corneal epithelial (HCE-T) cells are cultured within specialized cell holders and friction experiments are performed with a micro-tribometer. Cell damage is assessed via microscopy and a trypan blue viability assay. Preliminary experim

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Masters, Barry R. "Optical Biopsy of Ocular Tissue with Two-Photon Excitation Laser Scanning Microscopy." In Biomedical Optical Spectroscopy and Diagnostics. Washington, D.C.: Optica Publishing Group, 2006. http://dx.doi.org/10.1364/bosd.1996.ft7.

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Two-photon excitation laser scanning microscopy is used to produce three-dimensional maps of cellular metabolism based on the fluorescence of the naturally occurring reduced pyridine nucleotides NAD(P)H. The fluorescence from NAD(P)H was imaged with submicron lateral resolution through the 400 micron thickness of the cornea. Metabolic imaging with two-photon excitation scanning laser microscopy with near-infrared excitation has several advantages over conventional ultraviolet light. The near infrared light can penetrate deeper into the ocular tissue, there is reduced photodamage, and the chrom

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Mauck, Robert L., Pen-hsiu G. Chao, Beth Gilbert, Wilmot B. Valhmu, and Clark T. Hung. "Chondrocyte Translocation and Orientation to Applied DC Electric Fields." In ASME 1999 International Mechanical Engineering Congress and Exposition. American Society of Mechanical Engineers, 1999. http://dx.doi.org/10.1115/imece1999-0405.

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Abstract Chemical and mechanical stimuli are known to cause directed movement in a number of different cell types. Less prominently studied, direct current (DC) electric fields are known to induce a similar response. In this study, we report on DC electric field-induced chondrocyte migration and re-orientation. Galvanotaxis and galvanotropism, migration and shape change in response to applied DC electric fields, respectively, have been demonstrated in many cells. For instance, field strengths of 1–10 V/cm have been reported to induce migration in keratinocytes. corneal epithelial cells, bone c

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