Thematische Bibliographien / ECM proteiny

Inhaltsverzeichnis

  1. Zeitschriftenartikel
  2. Dissertationen
  3. Bücher
  4. Buchteile
  5. Konferenzberichte
  6. Berichte der Organisationen

Auswahl der wissenschaftlichen Literatur zum Thema „ECM proteiny“

Autor: Grafiati
Veröffentlicht am 28. Juni 2021
Zuletzt aktualisiert am 29. Juli 2025

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Zeitschriftenartikel zum Thema "ECM proteiny"

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Liu, Binghui, Ling Leng, Xuer Sun, Yunfang Wang, Jie Ma, and Yunping Zhu. "ECMPride: prediction of human extracellular matrix proteins based on the ideal dataset using hybrid features with domain evidence." PeerJ 8 (April 29, 2020): e9066. http://dx.doi.org/10.7717/peerj.9066.

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Extracellular matrix (ECM) proteins play an essential role in various biological processes in multicellular organisms, and their abnormal regulation can lead to many diseases. For large-scale ECM protein identification, especially through proteomic-based techniques, a theoretical reference database of ECM proteins is required. In this study, based on the experimentally verified ECM datasets and by the integration of protein domain features and a machine learning model, we developed ECMPride, a flexible and scalable tool for predicting ECM proteins. ECMPride achieved excellent performance in pr
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Papadimitriou, E., V. G. Manolopoulos, G. T. Hayman, et al. "Thrombin modulates vectorial secretion of extracellular matrix proteins in cultured endothelial cells." American Journal of Physiology-Cell Physiology 272, no. 4 (1997): C1112—C1122. http://dx.doi.org/10.1152/ajpcell.1997.272.4.c1112.

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We have identified a novel cellular action of thrombin on cultured rat adrenal medullary endothelial cells (RAMEC). Five-minute incubation of RAMEC with physiological concentrations of thrombin (<1 U/ml) caused within 3 h an increase in the basolateral deposition of the extracellular matrix (ECM) proteins fibronectin, laminin, and collagens IV and I, concomitant with a corresponding decrease in the apical release of these proteins into the medium. This shift in vectorial secretion of ECM proteins, quantitated with enzyme-linked immunoassays, was time dependent. Maximal stimulation of ECM pr
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El-Nakhel, Christophe, Eugenio Cozzolino, Lucia Ottaiano, et al. "Effect of Biostimulant Application on Plant Growth, Chlorophylls and Hydrophilic Antioxidant Activity of Spinach (Spinacia oleracea L.) Grown under Saline Stress." Horticulturae 8, no. 10 (2022): 971. http://dx.doi.org/10.3390/horticulturae8100971.

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Irrigated agricultural lands are prone to salinity problems which may imperil horticultural crops by reducing growth, yield and even qualitative traits. Eco-friendly approaches such as biostimulant application and in particular protein hydrolysates from vegetal origin are implemented to mitigate salinity stress effects on crops. For this reason, a greenhouse experiment on spinach irrigated with increasing concentrations of saline water (EC = 3 dS m−1 (EC3), 6 dS m−1 (EC6) and 9 dS m−1 (EC9), in addition to non-saline treatment (EC0)) was organized, while plants were subjected to foliar applica
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Rende, Umut, Seong Beom Ahn, Subash Adhikari, et al. "Deciphering the Kidney Matrisome: Identification and Quantification of Renal Extracellular Matrix Proteins in Healthy Mice." International Journal of Molecular Sciences 24, no. 3 (2023): 2827. http://dx.doi.org/10.3390/ijms24032827.

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Precise characterization of a tissue’s extracellular matrix (ECM) protein composition (matrisome) is essential for biomedicine. However, ECM protein extraction that requires organ-specific optimization is still a major limiting factor in matrisome studies. In particular, the matrisome of mouse kidneys is still understudied, despite mouse models being crucial for renal research. Here, we comprehensively characterized the matrisome of kidneys in healthy C57BL/6 mice using two ECM extraction methods in combination with liquid chromatography tandem mass spectrometry (LC-MS/MS), protein identificat
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Liem, David A., Sanjana Murali, Dibakar Sigdel, et al. "Phrase mining of textual data to analyze extracellular matrix protein patterns across cardiovascular disease." American Journal of Physiology-Heart and Circulatory Physiology 315, no. 4 (2018): H910—H924. http://dx.doi.org/10.1152/ajpheart.00175.2018.

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Extracellular matrix (ECM) proteins have been shown to play important roles regulating multiple biological processes in an array of organ systems, including the cardiovascular system. Using a novel bioinformatics text-mining tool, we studied six categories of cardiovascular disease (CVD), namely, ischemic heart disease, cardiomyopathies, cerebrovascular accident, congenital heart disease, arrhythmias, and valve disease, anticipating novel ECM protein-disease and protein-protein relationships hidden within vast quantities of textual data. We conducted a phrase-mining analysis, delineating the r
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Di Mola, Ida, Stefano Conti, Eugenio Cozzolino, et al. "Plant-Based Protein Hydrolysate Improves Salinity Tolerance in Hemp: Agronomical and Physiological Aspects." Agronomy 11, no. 2 (2021): 342. http://dx.doi.org/10.3390/agronomy11020342.

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Hemp (Cannabis sativa L.) is a multipurpose plant attracting increasing interest as a source for the production of natural fibers, paper, bio-building material and food. In this research we studied the agronomical performance of Cannabis sativa cv. Eletta Campana irrigated with saline water. Under those conditions, we tested the effect of protein hydrolysate (PH) biostimulant application in overcoming and/or balancing deleterious salinity effects. The results of the diverse treatments were also investigated at the physiological level, focusing on photosynthesis by means of a chlorophyll a fluo
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Hoke, David E., Suhelen Egan, Paul A. Cullen, and Ben Adler. "LipL32 Is an Extracellular Matrix-Interacting Protein of Leptospira spp. and Pseudoalteromonas tunicata." Infection and Immunity 76, no. 5 (2008): 2063–69. http://dx.doi.org/10.1128/iai.01643-07.

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ABSTRACT LipL32 is the major outer membrane protein in pathogenic Leptospira. It is highly conserved throughout pathogenic species and is expressed in vivo during human infection. While these data suggest a role in pathogenesis, a function for LipL32 has not been defined. Outer membrane proteins of gram-negative bacteria are the first line of molecular interaction with the host, and many have been shown to bind host extracellular matrix (ECM). A search for leptospiral ECM-interacting proteins identified the major outer membrane protein, LipL32. To verify this finding, recombinant LipL32 was ex
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Vallet, Sylvain D., Martin N. Davis, Anna Barqué, Ali H. Thahab, Sylvie Ricard-Blum, and Alexandra Naba. "Computational and experimental characterization of the novel ECM glycoprotein SNED1 and prediction of its interactome." Biochemical Journal 478, no. 7 (2021): 1413–34. http://dx.doi.org/10.1042/bcj20200675.

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The extracellular matrix (ECM) is a complex meshwork of proteins and an essential component of multicellular life. We have recently reported the characterization of a novel ECM protein, SNED1, and showed that it promotes breast cancer metastasis and regulates craniofacial development. However, the mechanisms by which it does so remain unknown. ECM proteins exert their functions by binding to cell surface receptors and interacting with other ECM proteins, actions that we can predict using knowledge of protein's sequence, structure, and post-translational modifications. Here, we combined in-sili
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Chan, Gek Cher, Diana G. Eng, Jeffrey H. Miner, et al. "Differential expression of parietal epithelial cell and podocyte extracellular matrix proteins in focal segmental glomerulosclerosis and diabetic nephropathy." American Journal of Physiology-Renal Physiology 317, no. 6 (2019): F1680—F1694. http://dx.doi.org/10.1152/ajprenal.00266.2019.

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In healthy glomeruli, parietal epithelial cell (PEC)-derived extracellular matrix (ECM) proteins include laminin-β1, perlecan, and collagen type IV-α2 and podocyte-specific ECM proteins include laminin-β2, agrin, and collagen type IV-α4. This study aimed to define individual ECM protein isoform expression by PECs in both experimental and human focal segmental glomerulosclerosis (FSGS) and diabetic nephropathy (DN) and to determine if changes were CD44 dependent. In experimental FSGS induced with a cytotoxic podocyte antibody and in the BTBR ob/ob mouse model of DN, PEC-derived protein staining
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Lipp, Sarah N., Kathryn R. Jacobson, David S. Hains, Andrew L. Schwarderer, and Sarah Calve. "3D Mapping Reveals a Complex and Transient Interstitial Matrix During Murine Kidney Development." Journal of the American Society of Nephrology 32, no. 7 (2021): 1649–65. http://dx.doi.org/10.1681/asn.2020081204.

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BackgroundThe extracellular matrix (ECM) is a network of proteins and glycosaminoglycans that provides structural and biochemical cues to cells. In the kidney, the ECM is critical for nephrogenesis; however, the dynamics of ECM composition and how it relates to 3D structure during development is unknown.MethodsUsing embryonic day 14.5 (E14.5), E18.5, postnatal day 3 (P3), and adult kidneys, we fractionated proteins based on differential solubilities, performed liquid chromatography–tandem mass spectrometry, and identified changes in ECM protein content (matrisome). Decellularized kidneys were
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Dissertationen zum Thema "ECM proteiny"

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Dvořák, Pavel. "Biomedicínské aplikace polykaprolaktonových nanovlákenných membrán." Master's thesis, Vysoké učení technické v Brně. Fakulta chemická, 2021. http://www.nusl.cz/ntk/nusl-444549.

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The diploma thesis deals with the treatment of polycaprolactone (PCL) nanofibers. PCL fibers were subjected to the deposition of plasma amine polymers in a low pressure pulsed radiofrequency capacitively coupled discharge using cyclopropylamine monomer (CPA). Collagen as an extracellular matrix (ECM) protein was immobilized and cell proliferation on the modified nanofiber surface was monitored. Untreated PCL fibers were also subjected to the deposition of an antibacterial copper layer, and the fibers were characterized by scanning electron microscopy (SEM), X-ray photoelectron spectroscopy (XP
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Tan, Yu Pei. "The development of Lactococcus lactis as an antimicrobial agent." Thesis, Queensland University of Technology, 2010. https://eprints.qut.edu.au/39143/1/Yu_Pei_Tan_Thesis.pdf.

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Non-pathogenic lactic acid bacteria are economically important Gram-positive bacteria used extensively in the food industry. Due to their “generally regarded as safe” status, certain species from the genera Lactobacillus and Lactococcus are also considered desirable as candidates for the production and secretion of recombinant proteins, particular those with therapeutic applications. The hypothesis examined by this thesis is that Lactococcus lactis can be modified to be an effective antimicrobial agent. Therefore, the aims of this thesis were to investigate the optimisation of the express
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Gründel, Anne. "Funktion glykolytischer Enzyme von Mycoplasma pneumoniae in der Wirt-Erreger-Interaktion." Doctoral thesis, Saechsische Landesbibliothek- Staats- und Universitaetsbibliothek Dresden, 2016. http://nbn-resolving.de/urn:nbn:de:bsz:14-qucosa-213500.

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Mycoplasma pneumoniae ist ein parasitär lebendes Bakterium, das eine atypische Pneumonie beim Menschen verursacht. Aufgrund seiner geringen Genomgröße besitzt dieser Organismus einen eingeschränkten Metabolismus sowie eine limitierte Zahl an Pathogenitätsfaktoren. Dennoch ist dieser Mikroorganismus perfekt an seinen Wirt angepasst und es war zu vermuten, dass neben dem komplexen Adhäsionsapparat von M. pneumoniae auch glykolytische Enzyme eine Rolle bei der Interaktion mit humanen Zellen spielen. Diese Enzyme sind maßgeblich bei intrazellulär ablaufenden Stoffwechselprozessen beteiligt. Es wur
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Owen, Jo. "Structural and functional studies of fibulin-1 EGF domains." Thesis, University of Oxford, 2002. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.270656.

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McCosker, Helen Clare. "Prognostic significance of IGF and ECM induced signalling proteins in breast cancer patients." Thesis, Queensland University of Technology, 2012. https://eprints.qut.edu.au/53580/1/Helen_McCosker_Thesis.pdf.

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Breast cancer is a leading contributor to the burden of disease in Australia. Fortunately, the recent introduction of diverse therapeutic strategies have improved the survival outcome for many women. Despite this, the clinical management of breast cancer remains problematic as not all approaches are sufficiently sophisticated to take into account the heterogeneity of this disease and are unable to predict disease progression, in particular, metastasis. As such, women with good prognostic outcomes are exposed to the side effects of therapies without added benefit. Furthermore, women with aggres
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López, Ceballos Pablo. "Elucidating how protein turnover in cell-ECM adhesion stabilizes tissue structure during development." Thesis, University of British Columbia, 2016. http://hdl.handle.net/2429/57622.

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Morphogenesis is the process by which cells rearrange to form complex three dimensional structures. Cell to extracellular matrix (ECM) adhesion, primarily mediated by Integrins, is essential for the formation and maintenance of tissue architecture. A critical way to regulate cell-ECM adhesion is by modulating the turnover of Integrins and their adhesion complex, and thereby modulating the stability of Integrin-based adhesions. We previously showed that mechanical force stabilizes Integrin-based adhesions during development by modulating Integrin turnover. Here, we extend our studies to underst
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Osta, Muhammad Samir Ahmed. "Characterisation of ECM protein processing mechanisms underlying simple peritoneal sclerosis and encapsulating peritoneal sclerosis." Thesis, University of Sheffield, 2015. http://etheses.whiterose.ac.uk/11406/.

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Introduction and hypothesis: Peritoneal dialysis (PD) is an important option for renal replacement therapy. Peritoneal sclerosis (PS) limits PD duration due to loss of ultrafiltration (UF) capacity, while about 3% of PD patients experience a condition termed encapsulating peritoneal sclerosis (EPS). In many fibrotic diseases reduced Extracellular matrix (ECM) breakdown due to lowered matrix metalloproteinase (MMP) activity occurs, often from over-expression of tissue inhibitors of MMP (TIMPs) that underlie fibrotic remodeling. Furthermore, recent application of 2D gel proteomics on peritoneal
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Gandhi, Neha Sureshchandra. "Molecular modelling of the interactions of complex carbohydrates with proteins." Thesis, Curtin University, 2011. http://hdl.handle.net/20.500.11937/2027.

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Glycosaminoglycans (GAGs) are ubiquitous complex carbohydrate molecules present on the cell surfaces and in extracellular matrices (ECM) of vertebrate and invertebrate tissues. The interactions of sulphated GAGs such as heparin and heparan sulphate (HS) with numerous immunologically-relevant proteins is now attracting considerable interest as a source of new therapeutics for the treatment of infectious diseases, inflammation and allergies, and cancers. Various computational molecular modelling methods are being employed to determine the nature of the interactions of heparin oligosaccharides wi
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Lundberg, Ida. "Fibroblasts and ECM in colorectal cancer : Analysis of subgroup specific protein expression and matrix arrangement." Thesis, Umeå universitet, Patologi, 2012. http://urn.kb.se/resolve?urn=urn:nbn:se:umu:diva-85606.

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The tumor microenvironment is important for tumor growth and progression, where the cancer associated fibroblast (CAF) is one central cell type. The CpG island methylator phenotype (CIMP) divides colorectal cancer (CRC) into three subgroups and in this study we investigate how the different CIMP-groups and adjacent fibroblasts affect each other. This was done with the aim of finding CIMP-specific markers and to see if different tumor-fibroblast interactions result in differently invasive tumors. Here we report that CIMP-negative tumors have increased expression of fibronectin, while CIMP-high
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Waters, Timothy Richard. "Studies on the Eco RV restriction endonuclease using oligodeoxynucleotides containing modified bases." Thesis, University of Southampton, 1992. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.385336.

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Bücher zum Thema "ECM proteiny"

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Manchenko, Gennady P. Handbook of detection of enzymes on electrophoretic gels. 2nd ed. CRC Press, 2002.

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Manchenko, Gennady P. Handbook of detection of enzymes on electrophoretic gels. CRC Press, 1994.

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Hannover, Universität, ed. Protein Engineering von Enzymvarianten des extended chain Motivs der Restriktionsendonuklease Eco RI. 1994.

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Lennon, Rachel, and Neil Turner. The molecular basis of glomerular basement membrane disorders. Edited by Neil Turner. Oxford University Press, 2018. http://dx.doi.org/10.1093/med/9780199592548.003.0320_update_001.

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The glomerular basement membrane (GBM) is a condensed network of extracellular matrix molecules which provides a scaffold and niche to support the function of the overlying glomerular cells. Within the glomerulus, the GBM separates the fenestrated endothelial cells, which line capillary walls from the epithelial cells or podocytes, which cover the outer aspect of the capillaries. In common with basement membranes throughout the body, the GBM contains core components including collagen IV, laminins, nidogens, and heparan sulphate proteoglycans. However, specific isoforms of these proteins are r
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Oelgeschläger, Thomas. Protein-Engineering an der Restriktionsendonuklease Eco RI: Konstruktion von Enzymvarianten mit erweiterter Substratspezifität. 1992.

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Moura, Rosa Maria Braga Lopes de. Tratamentos da Doença de Alzheimer: Perspectivas e Bioética. Edited by Marcia Alessandra Arantes Marques. Bookerfield Editora, 2021. http://dx.doi.org/10.53268/bkf21090500.

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A Doença de Alzheimer (DA) é um distúrbio neurodegenerativo progressivo associado com um acúmulo de placas da proteína beta amiloide (Aβ) senis extracelulares e o excesso de emaranhados neurofibrilares da proteína Tau dentro das células. Foi descrita inicialmente pelo médico alemão Alois Alzheimer, em 1906 caracterizando como uma patologia neurológica, de etiologia desconhecida e de aparecimento insidioso. O sistema nervoso central é particularmente vulnerável aos danos causados pelos radicais livres por apresentar alto consumo de oxigênio e possuir grande quantidade de ácidos graxos poli-insa
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Moura, Rosa Maria Braga Lopes de. Tratamentos da Doença de Alzheimer: Perspectivas e Bioética. Edited by Marcia Alessandra Arantes Marques. Bookerfield Editora, 2021. http://dx.doi.org/10.53268/bkf21090500.

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A Doença de Alzheimer (DA) é um distúrbio neurodegenerativo progressivo associado com um acúmulo de placas da proteína beta amiloide (Aβ) senis extracelulares e o excesso de emaranhados neurofibrilares da proteína Tau dentro das células. Foi descrita inicialmente pelo médico alemão Alois Alzheimer, em 1906 caracterizando como uma patologia neurológica, de etiologia desconhecida e de aparecimento insidioso. O sistema nervoso central é particularmente vulnerável aos danos causados pelos radicais livres por apresentar alto consumo de oxigênio e possuir grande quantidade de ácidos graxos poli-insa
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Aparecido da, Fábio, Ana Kelly Kapp Poli Schneider, and Renato Massaharu Hassunuma. CARTAG: livro-jogo sobre o código genético & alterações cromossômicas estruturais. Canal 6 Editora, 2022. http://dx.doi.org/10.52050/9788579175794.

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O termo CÓDIGO GENÉTICO refere-se à relação que existe entre a sequência de bases nitrogenadas do ácido desoxirribonucleico (DNA) e a sequência correspondente de aminoácidos em uma proteína. Este LIVRO-JOGO tem como proposta ensinar para alunos de diferentes níveis de ensino por meio de cartas, como entender o código genético e algumas das principais alterações cromossômicas estruturais. O CARTAG é um jogo formado por uma ficha de consulta do código genético e três baralhos: um de códons, outro de aminoácidos e um último de alterações cromossômicas estruturais.
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Dantas Filho, Jerônimo Vieira, Paulo Henrique Gilio Gasparotto, Jucilene Cavali, and Sandro de Vargas Schons. Tecnologias agropecuárias e ambientais : revisões e aplicações. Brazilian Journals Editora, 2022. http://dx.doi.org/10.35587/brj.ed.0001698.

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Trata-se de uma obra transdisciplinar, com enfoque na aplicação de agrotecnologias e tecnologias da informação às Ciências Agrárias, mais especificamente abordando o emprego e difusão de tecnologias no Agronegócio, com a finalidade de proporcionar Sustentabilidade nos sistemas de produção animal. A publicação contempla várias áreas de conhecimento, Engenharia de Pesca e Aquicultura, Sistemas de Informação, Medicina Veterinária, Zootecnia, etc. E, está organizada em dez capítulos, 1. Tecnologias da informação aplicadas à Engenharia de Pesca e Aquicultura, 2. Carne mecanicamente separada de pesc
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Handbook of detection of enzymes on electrophoretic gels. 2nd ed. CRC Press, 2003.

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Buchteile zum Thema "ECM proteiny"

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Gholami, Reza, and Seyed Morteza Naghib. "Bone ECM Proteins—Part I." In Biological and Medical Physics, Biomedical Engineering. Springer Nature Singapore, 2025. https://doi.org/10.1007/978-981-96-0041-0_5.

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Gholami, Reza, and Seyed Morteza Naghib. "Bone ECM Proteins—Part II." In Biological and Medical Physics, Biomedical Engineering. Springer Nature Singapore, 2025. https://doi.org/10.1007/978-981-96-0041-0_6.

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Brancaccio, Mara, Emila Turco, and Emilio Hirsch. "Tissue-Specific KO of ECM Proteins." In Methods in Molecular Biology. Humana Press, 2009. http://dx.doi.org/10.1007/978-1-59745-413-1_2.

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Ghorbani, Farnaz, Niyousha Davari, Chaozong Liu, and Behafarid Ghalandari. "Advances in ECM Protein-Based Materials." In Handbook of the Extracellular Matrix. Springer International Publishing, 2024. http://dx.doi.org/10.1007/978-3-031-56363-8_11.

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Ghorbani, Farnaz, Niyousha Davari, Chaozong Liu, and Behafarid Ghalandari. "Advances in ECM Protein-Based Materials." In Handbook of the Extracellular Matrix. Springer International Publishing, 2023. http://dx.doi.org/10.1007/978-3-030-92090-6_11-1.

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Mould, Paul A. "Solid Phase Assays for Studying ECM Protein–Protein Interactions." In Methods in Molecular Biology. Humana Press, 2009. http://dx.doi.org/10.1007/978-1-59745-413-1_13.

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Wang, Jiangxue, Ying Hou, and Jiawei Ma. "Titanium surfaces, biochemical modification by peptides and ECM proteins." In Encyclopedia of Metalloproteins. Springer New York, 2013. http://dx.doi.org/10.1007/978-1-4614-1533-6_200001.

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Smits, Daan, and Antoine A. Khalil. "Multimodal Techniques to Study Tumor Growth, Basement Membrane Breaching, and Invasion in 3D Matrices." In Cell Migration in Three Dimensions. Springer US, 2023. http://dx.doi.org/10.1007/978-1-0716-2887-4_17.

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AbstractCancer-derived organoids and three-dimensional (3D) extracellular matrix (ECM) are taking center stage as in vitro models to study neoplastic cell behavior, since they recapitulate the heterogeneous cellular composition of tumors and their extracellular environment. In combination with imaging and molecular/biochemical techniques, 3D organoid models have contributed substantially to our knowledge about the cellular and molecular mechanisms that regulate the growth of tumors and invasion into the surrounding tissue. We here outline a set of protocols that describe culturing of cancer-de
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Elagamey, Eman, Kanika Narula, Niranjan Chakraborty, and Subhra Chakraborty. "Extracellular Matrix Proteome: Isolation of ECM Proteins for Proteomics Studies." In Nitrogen Metabolism in Plants. Springer New York, 2019. http://dx.doi.org/10.1007/978-1-4939-9790-9_14.

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Paardekooper, E. J. C., and J. Bol. "Future protein foods." In Product Innovation and Eco-efficiency. Springer Netherlands, 1998. http://dx.doi.org/10.1007/978-94-015-8945-1_10.

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Konferenzberichte zum Thema "ECM proteiny"

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Wang, Yunjie, and Katherine Yanhang Zhang. "The Biomechanical Properties of Arterial Elastin With Glucose Effect." In ASME 2013 Summer Bioengineering Conference. American Society of Mechanical Engineers, 2013. http://dx.doi.org/10.1115/sbc2013-14200.

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Elastin, as one of the major extracellular matrix (ECM) components, is essential to accommodate physiological deformation and provide elastic support for blood vessels. Elastin is a long-lived ECM protein and it can suffer from cumulative effects of exposure to chemical damage, which can greatly compromise its biomechanical properties. The mechanical properties of elastin are related to its microstructure and the chemical environment. Glucose is an important carbohydrate in human body. The effect of glucose on the mechanical properties of blood vessels is especially magnified in diabetic patie
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Lee, Hae-Jeong, Marvi A. Matos, Lisa Pakstis, Marcus T. Cicerone, and Joy P. Dunkers. "Quantitation of Laminin Adsorbed Onto Polydimethylsiloxane Surfaces Using Various Treatment Protocols." In ASME 2008 Summer Bioengineering Conference. American Society of Mechanical Engineers, 2008. http://dx.doi.org/10.1115/sbc2008-192785.

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There is considerable interest in how cells respond to mechanical stimuli, from the ligands used to transmit the stimulus to the signaling pathways initiated and the proteins expressed upon phenotype change [1]. Previous work focused on the evaluation of the quality of the extracellular matrix (ECM) coating and cell proliferation [2]. Our focus is the characterization of a flexible polymeric substrate, treated with ECM, used to induce tensile strain on cells. In this work, we expand our physical characterization of the protein modified polydimethylsiloxane (PDMS) surface by quantifying the cov
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Sewell-Loftin, M. K., and W. David Merryman. "The Role of SRC in Strain- and Ligand- Dependent Phenotypic Modulation of Mouse Embryonic Fibroblasts." In ASME 2011 Summer Bioengineering Conference. American Society of Mechanical Engineers, 2011. http://dx.doi.org/10.1115/sbc2011-53604.

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Connective tissue fibrosis represents a significant portion of mortality and morbidity in our society. These diseases include many illnesses such as heart valve disease, atherosclerosis, macular degeneration, and cirrhosis, meaning that millions of lives are affected by these conditions each year. Fibrotic tissues form when quiescent fibroblasts activate becoming myofibroblasts, the phenotype of active tissue construction and fibrosis. During this process, the cells produce smooth muscle α-actin (αSMA), a contractile element considered to be the hallmark of cellular activation [1]. Following t
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Freytes, Donald O., Samuel Kolman, Sachin S. Velankar, and Stephen F. Badylak. "Rheological Properties of Extracellular Matrix Derived Gels." In ASME 2007 Summer Bioengineering Conference. American Society of Mechanical Engineers, 2007. http://dx.doi.org/10.1115/sbc2007-176537.

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Bioscaffolds composed of extracellular matrix (ECM) have been used for the repair of a variety of tissues often leading to tissue-specific constructive remodeling [1]. ECM scaffolds are typically prepared by decellularization of tissues and are composed of the structural proteins (e.g. collagen) and functional proteins (e.g. growth factors) that characterize the native ECM. However, for certain applications, the use of ECM scaffolds can be limited by the native two-dimensional sheet form in which they are harvested.
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Lai, Victor K., Edward A. Sander, Spencer P. Lake, Robert T. Tranquillo, and Victor H. Barocas. "Collagen Network Topology is Influenced by Collagen Concentration, But Not by Co-Gelation With Fibrin." In ASME 2011 Summer Bioengineering Conference. American Society of Mechanical Engineers, 2011. http://dx.doi.org/10.1115/sbc2011-53239.

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Extracellular matrix (ECM) proteins (e.g. collagen, elastin) play an important role in biological tissues. In addition to conferring mechanical strength to a tissue, the ECM provides a biochemical environment essential for modulation of cellular responses such as growth and migration. Collagens are the dominant protein of the ECM, with collagen type I being most abundant. Our group and others have shown that the mechanical properties of a collagen I matrix change with collagen concentration, and when formed in the presence of a secondary fibril network such as fibrin [1]. We are interested in
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Espinosa, Gabriela, Lisa Bennett, William Gardner, and Jessica Wagenseil. "The Effects of Extracellular Matrix Protein Insufficiency and Treatment on the Stiffness of Arterial Smooth Muscle Cells." In ASME 2013 Summer Bioengineering Conference. American Society of Mechanical Engineers, 2013. http://dx.doi.org/10.1115/sbc2013-14131.

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Increased arterial stiffness is directly correlated with hypertension and cardiovascular disease. Stiffness of the conducting arteries is largely determined by the extracellular matrix (ECM) proteins in the wall, such as collagen and elastin, produced by the smooth muscle cells (SMCs) found in the medial layer. Elastin is deposited as soluble tropoelastin and is later crosslinked into elastin fibers. Newborn mice lacking the elastin protein ( Eln−/−) have increased arterial wall stiffness and SMCs with altered proliferation, migration and morphology [1]. Vessel elasticity is also mediated by o
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Qiu, Weiguo, Arjun Stokes, Joseph Cappello, and Xiaoyi Wu. "Electrospinning of Recombinant Protein Polymer Nanofibers." In ASME 2009 Summer Bioengineering Conference. American Society of Mechanical Engineers, 2009. http://dx.doi.org/10.1115/sbc2009-206352.

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Structural proteins often in the form of micro and nanofibers, constituting most of intra- and extracellular matrix (ECM), are the fundamental building blocks of life [1]. Recent efforts to replace diseased or damaged tissues and organs have resulted in the molecular design and genetic engineering of recombinant proteins, and the advent of new technology for fabricating structural proteins into micro-/nanofibrous scaffolds, hoping to resemble some or all the characteristics of ECM structure and function. The fabrication of such an ECM mimic may be an important step in engineering a functional
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Cheng, Jeffrey K., Victoria Le, Robert P. Mecham, and Jessica E. Wagenseil. "Mechanics and Modeling of Postnatal Arterial Development in Wild-Type and Elastin-Insufficient Mice." In ASME 2011 Summer Bioengineering Conference. American Society of Mechanical Engineers, 2011. http://dx.doi.org/10.1115/sbc2011-53140.

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Large arteries in vertebrates serve as elastic reservoirs that store a portion of the blood volume with systole and discharge it during diastole. This function is made possible by the combination of extracellular matrix (ECM) proteins deposited by the smooth muscle cells (SMCs) in the arterial wall. Elastin and collagen expression in mice is first detectable around embryonic day 14 and peaks around postnatal day (P) 14, returning to baseline levels by P30. During this time, pressure and cardiac output increase significantly before leveling off ∼P30 [1]. Hence, the protein amounts and consequen
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Chan, Roger W., Thomas H. Siegmund, Kai Zhang, Neeraj Tirunagari, and Min Fu. "Structural Constitutive Characterization of the Vocal Fold: Modeling the Fibrous and the Interstitial Matrix Proteins." In ASME 2004 International Mechanical Engineering Congress and Exposition. ASMEDC, 2004. http://dx.doi.org/10.1115/imece2004-61298.

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The extracellular matrix (ECM) of the human vocal fold is a highly specialized soft connective tissue with a layered microstructure that is optimally tuned for vibration and sound production in response to a unique set of biomechanical stimuli in vivo, including oscillation at amplitudes up to 3–4 mm at magnitudes of acceleration > 200g and at high frequencies (> 100Hz). The vocal fold ECM, commonly called the lamina propria or mucosa, consists of biomacromolecules of two major classes distributed in different densities: (1) fibrous proteins including collagen and elastin fibers
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Mumbi, Anne, and Karl Behrendt. "Life Cycle Assessment of Grass Protein: Evaluating Environmental Impacts and Sustainability Potential." In 16th International Conference on Applied Human Factors and Ergonomics (AHFE 2025). AHFE International, 2025. https://doi.org/10.54941/ahfe1006564.

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The increasing global demand for sustainable protein sources has driven interest in grass protein as an alternative to conventional animal- and plant-based proteins. This study evaluates the environmental impacts of grass protein production using an attributional Life Cycle Analysis (LCA) approach. The analysis employs the Pasture-to-Plate (P2P) mechanochemical process, which integrates multiple unit operations to optimize protein extraction and processing efficiency. The LCA follows a cradle-to-gate system boundary, assessing key environmental impact categories such as global warming potentia
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Berichte der Organisationen zum Thema "ECM proteiny"

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Barash, Itamar, J. Mina Bissell, Alexander Faerman, and Moshe Shani. Modification of Milk Composition via Transgenesis: The Role of the Extracellular Matrix in Regulating Transgene Expression. United States Department of Agriculture, 1995. http://dx.doi.org/10.32747/1995.7570558.bard.

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Altering milk composition via transgenesis depends on three main factors. (1) The availability of an efficient regulatory sequences for targeting transgene(s) to the mammary gland; (2) a reliable in vitro model to test the expression of transgenes prior to their introduction to the animal genome; and (3) better understanding of the major factors which determine the rate of gene expression and protein synthesis. The current studies provide the necessary means and knowledge to alter milk protein composition via transgenesis. The following specific goals were achieved: a: Identifying regulatory r
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Bercovier, Herve, and Ronald P. Hedrick. Diagnostic, eco-epidemiology and control of KHV, a new viral pathogen of koi and common carp. United States Department of Agriculture, 2007. http://dx.doi.org/10.32747/2007.7695593.bard.

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Original objectives and revisions-The proposed research included these original objectives: field validation of diagnostic tests (PCR), the development and evaluation of new sensitive tools (LC-PCR/TaqManPCR, antibody detection by ELISA) including their use to study the ecology and the epidemiology of KHV (virus distribution in the environment and native cyprinids) and the carrier status of fish exposed experimentally or naturally to KHV (sites of virus replication and potential persistence or latency). In the course of the study we completed the genome sequence of KHV and developed a DNA arra
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Meidan, Rina, and Joy Pate. Roles of Endothelin 1 and Tumor Necrosis Factor-A in Determining Responsiveness of the Bovine Corpus Luteum to Prostaglandin F2a. United States Department of Agriculture, 2004. http://dx.doi.org/10.32747/2004.7695854.bard.

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The corpus luteum (CL) is a transient endocrine gland that has a vital role in the regulation of the estrous cycle, fertility and the maintenance of pregnancy. In the absence of appropriate support, such as occurs during maternal recognition of pregnancy, the CL will regress. Prostaglandin F2a (PGF) was first suggested as the physiological luteolysin in ruminants several decades ago. Yet, the cellular mechanisms by which PGF causes luteal regression remain poorly defined. In recent years it became evident that the process of luteal regression requires a close cooperation between steroidogenic,
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Eldar, Avigdor, and Donald L. Evans. Streptococcus iniae Infections in Trout and Tilapia: Host-Pathogen Interactions, the Immune Response Toward the Pathogen and Vaccine Formulation. United States Department of Agriculture, 2000. http://dx.doi.org/10.32747/2000.7575286.bard.

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In Israel and in the U.S., Streptococcus iniae is responsible for considerable losses in various fish species. Poor understanding of its virulence factors and limited know-how-to of vaccine formulation and administration are the main reasons for the limited efficacy of vaccines. Our strategy was that in order to Improve control measures, both aspects should be equally addressed. Our proposal included the following objectives: (i) construction of host-pathogen interaction models; (ii) characterization of virulence factors and immunodominant antigens, with assessment of their relative importance
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Minz, Dror, Stefan J. Green, Noa Sela, Yitzhak Hadar, Janet Jansson, and Steven Lindow. Soil and rhizosphere microbiome response to treated waste water irrigation. United States Department of Agriculture, 2013. http://dx.doi.org/10.32747/2013.7598153.bard.

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Research objectives : Identify genetic potential and community structure of soil and rhizosphere microbial community structure as affected by treated wastewater (TWW) irrigation. This objective was achieved through the examination soil and rhizosphere microbial communities of plants irrigated with fresh water (FW) and TWW. Genomic DNA extracted from soil and rhizosphere samples (Minz laboratory) was processed for DNA-based shotgun metagenome sequencing (Green laboratory). High-throughput bioinformatics was performed to compare both taxonomic and functional gene (and pathway) differences betwee
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