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Thematische Bibliographien / In Vitro Compartmentalised Self Replication

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Auswahl der wissenschaftlichen Literatur zum Thema „In Vitro Compartmentalised Self Replication“

Autor: Grafiati

Veröffentlicht am 20. Oktober 2023

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Zeitschriftenartikel zum Thema "In Vitro Compartmentalised Self Replication"

1

Bansho, Yohsuke, Taro Furubayashi, Norikazu Ichihashi, and Tetsuya Yomo. "Host–parasite oscillation dynamics and evolution in a compartmentalized RNA replication system." Proceedings of the National Academy of Sciences 113, no. 15 (2016): 4045–50. http://dx.doi.org/10.1073/pnas.1524404113.

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To date, various cellular functions have been reconstituted in vitro such as self-replication systems using DNA, RNA, and proteins. The next important challenges include the reconstitution of the interactive networks of self-replicating species and investigating how such interactions generate complex ecological behaviors observed in nature. Here, we synthesized a simple replication system composed of two self-replicating host and parasitic RNA species. We found that the parasitic RNA eradicates the host RNA under bulk conditions; however, when the system is compartmentalized, a continuous osci

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2

Dramé-Maigné, Adèle, Anton S. Zadorin, Iaroslava Golovkova, and Yannick Rondelez. "Quantifying the Performance of Micro-Compartmentalized Directed Evolution Protocols." Life 10, no. 2 (2020): 17. http://dx.doi.org/10.3390/life10020017.

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High-throughput, in vitro approaches for the evolution of enzymes rely on a random micro-encapsulation to link phenotypes to genotypes, followed by screening or selection steps. In order to optimise these approaches, or compare one to another, one needs a measure of their performance at extracting the best variants of a library. Here, we introduce a new metric, the Selection Quality Index (SQI), which can be computed from a simple mock experiment, performed with a known initial fraction of active variants. In contrast to previous approaches, our index integrates the effect of random co-encapsu

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3

Mulaj, Mentor, Tatiana Miti, and Martin Muschol. "Self-Replication of Transthyretin Amyloid Aggregates from Native Tetramers in vitro." Biophysical Journal 108, no. 2 (2015): 45a. http://dx.doi.org/10.1016/j.bpj.2014.11.280.

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4

Wang, Li-juan, Hou-xiu Wang, Longhe Jiang, and Chun-yang Zhang. "Development of an in Vitro Autocatalytic Self-Replication System for Biosensing Application." ACS Sensors 3, no. 12 (2018): 2675–83. http://dx.doi.org/10.1021/acssensors.8b01171.

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5

Rupp, Brigitte, Zsolt Ruzsics, Torsten Sacher, and Ulrich H. Koszinowski. "Conditional Cytomegalovirus Replication In Vitro and In Vivo." Journal of Virology 79, no. 1 (2005): 486–94. http://dx.doi.org/10.1128/jvi.79.1.486-494.2005.

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ABSTRACT We have established a conditional gene expression system for cytomegalovirus which allows regulation of genes independently from the viral replication program. Due to the combination of all elements required for regulated expression in the same viral genome, conditional viruses can be studied in different cell lines in vitro and in the natural host in vivo. The combination of a self-sufficient tetracycline-regulated expression cassette and Flp recombinase-mediated insertion into the viral genome allowed fast construction of recombinant murine cytomegaloviruses carrying different condi

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6

Hwang, Yung, Melinda Futran, Daniel Hidalgo, Divya Ramalingam Iyer, Nicholas Rhind, and Merav Socolovsky. "Global Increase in Replication Fork Speed during a p57KIP2-Regulated Erythroid Cell Fate Switch." Blood 128, no. 22 (2016): 698. http://dx.doi.org/10.1182/blood.v128.22.698.698.

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Abstract Cell cycle regulators are increasingly implicated in cell fate decisions such as the acquisition or loss of pluripotency and self-renewal potential. The cell cycle mechanisms that regulate these cell fate decisions are largely unknown. Here we studied an S phase- dependent cell fate switch in the erythroid fetal liver, in which murine early erythroid progenitors transition in vivo from a self-renewal state into a phase of active erythroid gene transcription and concurrent maturational cell divisions. In the fetal liver, this transition corresponds to the transition from subset S0 (CD7

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7

Sugiyama, Kazuo, Kenji Suzuki, Takahide Nakazawa, et al. "Genetic Analysis of Hepatitis C Virus with Defective Genome and Its Infectivity in Vitro." Journal of Virology 83, no. 13 (2009): 6922–28. http://dx.doi.org/10.1128/jvi.02674-08.

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ABSTRACT Replication and infectivity of hepatitis C virus (HCV) with a defective genome is ambiguous. We molecularly cloned 38 HCV isolates with defective genomes from 18 patient sera. The structural regions were widely deleted, with the 5′ untranslated, core, and NS3-NS5B regions preserved. All of the deletions were in frame, indicating that they are translatable to the authentic terminus. Phylogenetic analyses showed self-replication of the defective genomes independent of full genomes. We generated a defective genome of chimeric HCV to mimic the defective isolate in the serum. By using this

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8

Zitzmann, Carolin, Christopher Dächert, Bianca Schmid, et al. "Mathematical modeling of plus-strand RNA virus replication to identify broad-spectrum antiviral treatment strategies." PLOS Computational Biology 19, no. 4 (2023): e1010423. http://dx.doi.org/10.1371/journal.pcbi.1010423.

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Plus-strand RNA viruses are the largest group of viruses. Many are human pathogens that inflict a socio-economic burden. Interestingly, plus-strand RNA viruses share remarkable similarities in their replication. A hallmark of plus-strand RNA viruses is the remodeling of intracellular membranes to establish replication organelles (so-called “replication factories”), which provide a protected environment for the replicase complex, consisting of the viral genome and proteins necessary for viral RNA synthesis. In the current study, we investigate pan-viral similarities and virus-specific differenc

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Jain, Bhawana, Amita Jain, Om Prakash, et al. "In-vitro validation of self designed siRNA targeting non-structural 1 gene of Influenza A virus." South Asian Journal of Experimental Biology 4, no. 6 (2015): 315–22. http://dx.doi.org/10.38150/sajeb.4(6).p315-322.

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The genomic variability makes Influenza A virus (IAV) difficult to be con-trolled by existing vaccines or anti-influenza drugs. Viral gene targeting siRNA induces the RNAi mechanism in the host and silents the gene by cleaving mRNA. Objective was to develop siRNA targeting non-structural 1 gene and to validate its efficiency in vitro. siRNA was designed rationally, targeting the most conserved region (delineated with the help of multiple sequence align-ment) of NS1 gene of IAV strains. To choose the most efficient siRNA, three levels screening method was developed. Ultimately one siRNA duplex

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Bourne, Christina, Sejin Lee, Bollu Venkataiah, et al. "Small-Molecule Effectors of Hepatitis B Virus Capsid Assembly Give Insight into Virus Life Cycle." Journal of Virology 82, no. 20 (2008): 10262–70. http://dx.doi.org/10.1128/jvi.01360-08.

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ABSTRACT The relationship between the physical chemistry and biology of self-assembly is poorly understood, but it will be critical to quantitatively understand infection and for the design of antivirals that target virus genesis. Here we take advantage of heteroaryldihydropyrimidines (HAPs), which affect hepatitis B virus (HBV) assembly, to gain insight and correlate in vitro assembly with HBV replication in culture. Based on a low-resolution crystal structure of a capsid-HAP complex, a closely related series of HAPs were designed and synthesized. These differentially strengthen the associati

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