Thematische Bibliographien / Membranes, bilayers and vesicles

Inhaltsverzeichnis

Zeitschriftenartikel
Dissertationen
Bücher
Buchteile
Konferenzberichte

Auswahl der wissenschaftlichen Literatur zum Thema „Membranes, bilayers and vesicles“

Autor: Grafiati

Veröffentlicht am 17. September 2021

Zuletzt aktualisiert am 3. Februar 2022

Geben Sie eine Quelle nach APA, MLA, Chicago, Harvard und anderen Zitierweisen an

Wählen Sie eine Art der Quelle aus:

Machen Sie sich mit den Listen der aktuellen Artikel, Bücher, Dissertationen, Berichten und anderer wissenschaftlichen Quellen zum Thema "Membranes, bilayers and vesicles" bekannt.

Neben jedem Werk im Literaturverzeichnis ist die Option "Zur Bibliographie hinzufügen" verfügbar. Nutzen Sie sie, wird Ihre bibliographische Angabe des gewählten Werkes nach der nötigen Zitierweise (APA, MLA, Harvard, Chicago, Vancouver usw.) automatisch gestaltet.

Sie können auch den vollen Text der wissenschaftlichen Publikation im PDF-Format herunterladen und eine Online-Annotation der Arbeit lesen, wenn die relevanten Parameter in den Metadaten verfügbar sind.

Zeitschriftenartikel zum Thema "Membranes, bilayers and vesicles"

PLASENCIA, Ines, Luis RIVAS, Kevin M. W. KEOUGH, Derek MARSH und Jesús PÉREZ-GIL. „The N-terminal segment of pulmonary surfactant lipopeptide SP-C has intrinsic propensity to interact with and perturb phospholipid bilayers“. Biochemical Journal 377, Nr. 1 (01.01.2004): 183–93. http://dx.doi.org/10.1042/bj20030815.

Der volle Inhalt der Quelle

Annotation:

In the present study, 13-residue peptides with sequences corresponding to the native N-terminal segment of pulmonary SP-C (surfactant protein C) have been synthesized and their interaction with phospholipid bilayers characterized. The peptides are soluble in aqueous media but associate spontaneously with bilayers composed of either zwitterionic (phosphatidylcholine) or anionic (phosphatidylglycerol) phospholipids. The peptides show higher affinity for anionic than for zwitterionic membranes. Interaction of the peptides with both zwitterionic and anionic membranes promotes phospholipid vesicle aggregation, and leakage of the aqueous content of the vesicles. The lipid–peptide interaction includes a significant hydrophobic component for both zwitterionic and anionic membranes, although the interaction with phosphatidylglycerol bilayers is also electrostatic in nature. The effects of the SP-C N-terminal peptides on the membrane structure are mediated by significant perturbations of the packing order and mobility of phospholipid acyl chain segments deep in the bilayer, as detected by differential scanning calorimetry and spin-label ESR. These results suggest that the N-terminal region of SP-C, even in the absence of acylation, possesses an intrinsic propensity to interact with and perturb phospholipid bilayers, thereby potentially facilitating SP-C promoting bilayer-monolayer transitions at the alveolar spaces.

APA, Harvard, Vancouver, ISO und andere Zitierweisen

Martin, F. G., und M. G. Wolfersberger. „Bacillus thuringiensis delta-endotoxin and larval Manduca sexta midgut brush-border membrane vesicles act synergistically to cause very large increases in the conductance of planar lipid bilayers.“ Journal of Experimental Biology 198, Nr. 1 (01.01.1995): 91–96. http://dx.doi.org/10.1242/jeb.198.1.91.

Der volle Inhalt der Quelle

Annotation:

Brush-border membrane vesicles prepared from midguts of Manduca sexta larvae were incorporated into planar phospholipid bilayers. Addition of Bacillus thuringiensis delta-endotoxin to the buffered salt solutions bathing these bilayers resulted in large irreversible increases in conductance. At pH 9.6, the smallest toxin-dependent increase in bilayer conductance observed was 13 nS. Similar conductance increases were never observed in the absence of delta-endotoxin or in delta-endotoxin-treated bilayers not containing components of insect brush-border membranes.

APA, Harvard, Vancouver, ISO und andere Zitierweisen

Niles, W. D., und F. S. Cohen. „Video fluorescence microscopy studies of phospholipid vesicle fusion with a planar phospholipid membrane. Nature of membrane-membrane interactions and detection of release of contents.“ Journal of General Physiology 90, Nr. 5 (01.11.1987): 703–35. http://dx.doi.org/10.1085/jgp.90.5.703.

Der volle Inhalt der Quelle

Annotation:

Video fluorescence microscopy was used to study adsorption and fusion of unilamellar phospholipid vesicles to solvent-free planar bilayer membranes. Large unilamellar vesicles (2-10 microns diam) were loaded with 200 mM of the membrane-impermeant fluorescent dye calcein. Vesicles were ejected from a pipette brought to within 10 microns of the planar membrane, thereby minimizing background fluorescence and diffusion times through the unstirred layer. Vesicle binding to the planar membrane reached a maximum at 20 mM calcium. The vesicles fused when they were osmotically swollen by dissipating a KCl gradient across the vesicular membrane with the channel-forming antibiotic nystatin or, alternatively, by making the cis compartment hyperosmotic. Osmotically induced ruptures appeared as bright flashes of light that lasted several video fields (each 1/60 s). Flashes of light, and therefore swelling, occurred only when channels were present in the vesicular membrane. The flashes were observed when nystatin was added to the cis compartment but not when added to the trans. This demonstrates that the vesicular and planar membranes remain individual bilayers in the region of contact, rather than melding into a single bilayer. Measurements of flash duration in the presence of cobalt (a quencher of calcein fluorescence) were used to determine the side of the planar membrane to which dye was released. In the presence of 20 mM calcium, 50% of the vesicle ruptures were found to result in fusion with the planar membrane. In 100 mM calcium, nearly 70% of the vesicle ruptures resulted in fusion. The methods of this study can be used to increase significantly the efficiency of reconstitution of channels into planar membranes by fusion techniques.

APA, Harvard, Vancouver, ISO und andere Zitierweisen

Sessa, Lucia, Simona Concilio, Peter Walde, Tom Robinson, Petra S. Dittrich, Amalia Porta, Barbara Panunzi, Ugo Caruso und Stefano Piotto. „Study of the Interaction of a Novel Semi-Synthetic Peptide with Model Lipid Membranes“. Membranes 10, Nr. 10 (19.10.2020): 294. http://dx.doi.org/10.3390/membranes10100294.

Der volle Inhalt der Quelle

Annotation:

Most linear peptides directly interact with membranes, but the mechanisms of interaction are far from being completely understood. Here, we present an investigation of the membrane interactions of a designed peptide containing a non-natural, synthetic amino acid. We selected a nonapeptide that is reported to interact with phospholipid membranes, ALYLAIRKR, abbreviated as ALY. We designed a modified peptide (azoALY) by substituting the tyrosine residue of ALY with an antimicrobial azobenzene-bearing amino acid. Both of the peptides were examined for their ability to interact with model membranes, assessing the penetration of phospholipid monolayers, and leakage across the bilayer of large unilamellar vesicles (LUVs) and giant unilamellar vesicles (GUVs). The latter was performed in a microfluidic device in order to study the kinetics of leakage of entrapped calcein from the vesicles at the single vesicle level. Both types of vesicles were prepared from a 9:1 (mol/mol) mixture of POPC (1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine) and POPG (1-palmitoyl-2-oleoyl-sn-glycero-3-phospho(1′-rac-glycerol). Calcein leakage from the vesicles was more pronounced at a low concentration in the case of azoALY than for ALY. Increased vesicle membrane disturbance in the presence of azoALY was also evident from an enzymatic assay with LUVs and entrapped horseradish peroxidase. Molecular dynamics simulations of ALY and azoALY in an anionic POPC/POPG model bilayer showed that ALY peptide only interacts with the lipid head groups. In contrast, azoALY penetrates the hydrophobic core of the bilayers causing a stronger membrane perturbation as compared to ALY, in qualitative agreement with the experimental results from the leakage assays.

APA, Harvard, Vancouver, ISO und andere Zitierweisen

Neupane, Shova, George Cordoyiannis, Frank Uwe Renner und Patricia Losada-Pérez. „Real-Time Monitoring of Interactions between Solid-Supported Lipid Vesicle Layers and Short- and Medium-Chain Length Alcohols: Ethanol and 1-Pentanol“. Biomimetics 4, Nr. 1 (22.01.2019): 8. http://dx.doi.org/10.3390/biomimetics4010008.

Der volle Inhalt der Quelle

Annotation:

Lipid bilayers represent the interface between the cell and its environment, serving as model systems for the study of various biological processes. For instance, the addition of small molecules such as alcohols is a well-known process that modulates lipid bilayer properties, being considered as a reference for general anesthetic molecules. A plethora of experimental and simulation studies have focused on alcohol’s effect on lipid bilayers. Nevertheless, most studies have focused on lipid membranes formed in the presence of alcohols, while the effect of n-alcohols on preformed lipid membranes has received much less research interest. Here, we monitor the real-time interaction of short-chain alcohols with solid-supported vesicles of dipalmitoylphosphatidylcholine (DPPC) using quartz crystal microbalance with dissipation monitoring (QCM-D) as a label-free method. Results indicate that the addition of ethanol at different concentrations induces changes in the bilayer organization but preserves the stability of the supported vesicle layer. In turn, the addition of 1-pentanol induces not only changes in the bilayer organization, but also promotes vesicle rupture and inhomogeneous lipid layers at very high concentrations.

APA, Harvard, Vancouver, ISO und andere Zitierweisen

Tavares, G. D., M. C. de Oliveira, J. M. C. Vilela und M. S. Andrade. „Deposition of Lipid Bilayers with Atomic Force Microscopy“. Microscopy and Microanalysis 11, S03 (Dezember 2005): 44–47. http://dx.doi.org/10.1017/s1431927605050853.

Der volle Inhalt der Quelle

Annotation:

Biological membranes are constituted of lipids organized as a two dimensional bilayer supporting peripheral and integral proteins, providing a barrier between the inside and the outside of a cell [1]. Similar membranes can be prepared from the lipid mixtures forming liposomes. The liposomes are multi or unilamellar spherical vesicles in which an aqueous volume is enclosed and can be used to encapsulate some drugs [2]. In order to better expose the details of their structure, these membranes are generally deposited on the surface of a flat substrate. These supported planar lipid membranes can also provide a model system for investigating the properties and functions of the complex cell membrane and membrane mediated processes such as recognition events and biological signal transduction. Various methods have been used to create artificial lipid membranes supported on a solid surface, being the most used the Langmuir-Blodgett monolayers formation [3], the vesicle fusion or liposome adsorption [4] and the solution spreading [5].

APA, Harvard, Vancouver, ISO und andere Zitierweisen

Zheng, Hui, Sungsoo Lee, Marc C. Llaguno und Qiu-Xing Jiang. „bSUM: A bead-supported unilamellar membrane system facilitating unidirectional insertion of membrane proteins into giant vesicles“. Journal of General Physiology 147, Nr. 1 (28.12.2015): 77–93. http://dx.doi.org/10.1085/jgp.201511448.

Der volle Inhalt der Quelle

Annotation:

Fused or giant vesicles, planar lipid bilayers, a droplet membrane system, and planar-supported membranes have been developed to incorporate membrane proteins for the electrical and biophysical analysis of such proteins or the bilayer properties. However, it remains difficult to incorporate membrane proteins, including ion channels, into reconstituted membrane systems that allow easy control of operational dimensions, incorporation orientation of the membrane proteins, and lipid composition of membranes. Here, using a newly developed chemical engineering procedure, we report on a bead-supported unilamellar membrane (bSUM) system that allows good control over membrane dimension, protein orientation, and lipid composition. Our new system uses specific ligands to facilitate the unidirectional incorporation of membrane proteins into lipid bilayers. Cryo–electron microscopic imaging demonstrates the unilamellar nature of the bSUMs. Electrical recordings from voltage-gated ion channels in bSUMs of varying diameters demonstrate the versatility of the new system. Using KvAP as a model system, we show that compared with other in vitro membrane systems, the bSUMs have the following advantages: (a) a major fraction of channels are orientated in a controlled way; (b) the channels mediate the formation of the lipid bilayer; (c) there is one and only one bilayer membrane on each bead; (d) the lipid composition can be controlled and the bSUM size is also under experimental control over a range of 0.2–20 µm; (e) the channel activity can be recorded by patch clamp using a planar electrode; and (f) the voltage-clamp speed (0.2–0.5 ms) of the bSUM on a planar electrode is fast, making it suitable to study ion channels with fast gating kinetics. Our observations suggest that the chemically engineered bSUMs afford a novel platform for studying lipid–protein interactions in membranes of varying lipid composition and may be useful for other applications, such as targeted delivery and single-molecule imaging.

APA, Harvard, Vancouver, ISO und andere Zitierweisen

Kozlov, M. M., und V. S. Markin. „Elastic properties of membranes: Monolayers, bilayers, vesicles“. Journal of Colloid and Interface Science 138, Nr. 2 (September 1990): 332–45. http://dx.doi.org/10.1016/0021-9797(90)90216-b.

Der volle Inhalt der Quelle

APA, Harvard, Vancouver, ISO und andere Zitierweisen

Wang, Meina, Adriana M. Mihut, Ellen Rieloff, Aleksandra P. Dabkowska, Linda K. Månsson, Jasper N. Immink, Emma Sparr und Jérôme J. Crassous. „Assembling responsive microgels at responsive lipid membranes“. Proceedings of the National Academy of Sciences 116, Nr. 12 (01.03.2019): 5442–50. http://dx.doi.org/10.1073/pnas.1807790116.

Der volle Inhalt der Quelle

Annotation:

Directed colloidal self-assembly at fluid interfaces can have a large impact in the fields of nanotechnology, materials, and biomedical sciences. The ability to control interfacial self-assembly relies on the fine interplay between bulk and surface interactions. Here, we investigate the interfacial assembly of thermoresponsive microgels and lipogels at the surface of giant unilamellar vesicles (GUVs) consisting of phospholipids bilayers with different compositions. By altering the properties of the lipid membrane and the microgel particles, it is possible to control the adsorption/desorption processes as well as the organization and dynamics of the colloids at the vesicle surface. No translocation of the microgels and lipogels through the membrane was observed for any of the membrane compositions and temperatures investigated. The lipid membranes with fluid chains provide highly dynamic interfaces that can host and mediate long-range ordering into 2D hexagonal crystals. This is in clear contrast to the conditions when the membranes are composed of lipids with solid chains, where there is no crystalline arrangement, and most of the particles desorb from the membrane. Likewise, we show that in segregated membranes, the soft microgel colloids form closely packed 2D crystals on the fluid bilayer domains, while hardly any particles adhere to the more solid bilayer domains. These findings thus present an approach for selective and controlled colloidal assembly at lipid membranes, opening routes toward the development of tunable soft materials.

APA, Harvard, Vancouver, ISO und andere Zitierweisen

Talbot, T., F. Booy, R. D. Leapman und N. L. Gershfeld. „Cryo-Em of Large Unilamellar Phospholipid Vesicles That Self- Assemble At a Critical Temperature“. Microscopy and Microanalysis 7, S2 (August 2001): 714–15. http://dx.doi.org/10.1017/s1431927600029640.

Der volle Inhalt der Quelle

Annotation:

The mechanism for assembly of membrane lipid bilayers in vivo is generally considered to occur on existing membranes which act as templates for the assembly process. Not presently understood is the source of the membrane template. A thermodynamic theory describing a spontaneous process for assembly of unilamellar vesicles with properties of a critical state (1,2) has been tested in this study. The spontaneous formation of large unilamellar vesicles (LUV’s) from phospholipid components of cell membranes at a critical temperature T* has now been documented by cryo-electron microscope studies using aqueous dispersions of dimyrystoylphosphatidylcholine (T* = 29°C), and the total lipid extracts of E. coli membranes cultured at 32°C (T* = 32°C). The lipids were incubated at temperatures below, at and above the critical temperatures for LUV assembly. in conformity with thermodynamic theory, and other physical measurements that indicate unique bilayer properties exist at T* (3-5), LUV’s are seen to form only at T*.

APA, Harvard, Vancouver, ISO und andere Zitierweisen

Mehr Quellen

Dissertationen zum Thema "Membranes, bilayers and vesicles"

Bertrand, Martin. „Deformed Soft Matter under Constraints“. Thesis, Université d'Ottawa / University of Ottawa, 2012. http://hdl.handle.net/10393/20564.

Der volle Inhalt der Quelle

Annotation:

In the last few decades, an increasing number of physicists specialized in soft matter, including polymers, have turned their attention to biologically relevant materials. The properties of various molecules and fibres, such as DNA, RNA, proteins, and filaments of all sorts, are studied to better understand their behaviours and functions. Self-assembled biological membranes, or lipid bilayers, are also the focus of much attention as many life processes depend on these. Small lipid bilayers vesicles dubbed liposomes are also frequently used in the pharmaceutical and cosmetic industries. In this thesis, work is presented on both the elastic properties of polymers and the response of lipid bilayer vesicles to extrusion in narrow-channels. These two areas of research may seem disconnected but they both concern deformed soft materials. The thesis contains four articles: the first presenting a fundamental study of the entropic elasticity of circular chains; the second, a simple universal description of the effect of sequence on the elasticity of linear polymers such as DNA; the third, a model of the symmetric thermophoretic stretch of a nano-confined polymer; the fourth, a model that predicts the final sizes of vesicles obtained by pressure extrusion. These articles are preceded by an extensive introduction that covers all of the essential concepts and theories necessary to understand the work that has been done.

APA, Harvard, Vancouver, ISO und andere Zitierweisen

Harman, Alison. „A Molecular Dynamics Simulation of Vesicle Deformation and Rupture in Confined Poiseuille Flow“. Thèse, Université d'Ottawa / University of Ottawa, 2013. http://hdl.handle.net/10393/26127.

Der volle Inhalt der Quelle

Annotation:

Vesicles are simple structures, but display complex, non-linear dynamics in fluid flow. I investigate the deformation of nanometer-sized vesicles, both fully-inflated and those with excess area, as they travel in tightly confined capillaries. By varying both channel size and flow strength, I simulate vesicles as they transition from steady-state to unstable shapes, and then rupture in strong flow fields. By employing a molecular dynamics model of the vesicle, fluid, and capillary system one is able to rupture the lipid bilayer of these vesicles. This is unique in that most other numerical methods for modelling vesicles are unable to show rupture. The rupture of fully-inflated vesicles is applicable to drug delivery in which the release of the encapsulated medicine needs to be controlled. The deformation and rupture of vesicles with excess area could be applicable to red blood cells which have similar rheological properties.

APA, Harvard, Vancouver, ISO und andere Zitierweisen

Blochel, Andreas. „Adsorption of halogenated phenolate ions to egg-phosphatidylcholine vesicles“. PDXScholar, 1992. https://pdxscholar.library.pdx.edu/open_access_etds/4271.

Der volle Inhalt der Quelle

APA, Harvard, Vancouver, ISO und andere Zitierweisen

Kuhlmann, Jan Wilhelm. „Modulation of lateral membrane tension and SNARE-mediated single vesicle fusion on pore spanning membranes“. Doctoral thesis, Niedersächsische Staats- und Universitätsbibliothek Göttingen, 2017. http://hdl.handle.net/11858/00-1735-0000-0023-3F13-E.

Der volle Inhalt der Quelle

APA, Harvard, Vancouver, ISO und andere Zitierweisen

Norman, Robert Ellis. „Statistical mechanics of vesicles, membranes and interfaces“. Thesis, University of Southampton, 1993. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.358818.

Der volle Inhalt der Quelle

APA, Harvard, Vancouver, ISO und andere Zitierweisen

Lee, Lester Y. C. „Transmembrane electron transfer in artificial bilayers /“. Full text open access at:, 1985. http://content.ohsu.edu/u?/etd,86.

Der volle Inhalt der Quelle

APA, Harvard, Vancouver, ISO und andere Zitierweisen

Robinson, Alan Jonathan. „The computer simulation of lipid bilayers and biological membranes“. Thesis, University of Oxford, 1996. https://ora.ox.ac.uk/objects/uuid:787e13b4-4a3e-44ce-bd2d-9bb847631a5d.

Der volle Inhalt der Quelle

Annotation:

Computer simulations of lipid bilayers and biological membranes using molecular mechanics calculations have been undertaken in order to study these complex systems which are so vital in the control and functioning of many biological processes. The preliminary research involved the development of a model that recreates experimentally observed properties. This is not a trivial task since structural data on lipids in the biologically relevant liquid crystalline phase are unavailable precisely because of their fluid nature. The starting configuration designed for simulation of lipids in the fluid phase contained four different lipid conformations. These reflected the most probable head group and glycerol moiety conformations plus gauche dihedrals were introduced into the hydrocarbon chains so that they resembled chains in the fluid phase and reduced the time required for equilibration molecular dynamics. The bilayer model was then used to study cholesterol-lipid and peptide-lipid interactions. The cholesterol simulations illustrated how this molecule orders lipid chains by virtue of its rigid skeleton while the peptide simulations showed how cooperative the interactions between proteins and lipids are. Finally simulations of ion channels of gramicidin and melittin in membranes were accomplished and conclusions drawn on the nature and mechanism of the toxicity of melittin and of how water and ion translocation occurs along gramicidin channels.

APA, Harvard, Vancouver, ISO und andere Zitierweisen

Maleki, Karyak Mohsen. „Modeling and analysis of lipid bilayers with applications to vesicles and lipoprotein particles“. Thesis, McGill University, 2014. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=121455.

Der volle Inhalt der Quelle

Annotation:

Continuum approaches for modeling lipid bilayers are developed and applied to two-phase lipid vesicles and discoidal high-density lipoprotein (HDL) particles. First, relying on a three-dimensional model, the mechanics of a lipid bilayer with spontaneous curvature is considered. Kinematics, material symmetry, stress relations, and coherency of lipid bilayer leaflets are discussed. Treating a lipid bilayer as a thin structure, the areal energy density of a lipid bilayer with spontaneous curvature is obtained using a dimension-reduction procedure. Attention is paid on the source of spontaneous curvature in the well known Canham–Helfrich energy density. Also, the effect of constitutive asymmetry of the leaflets on the areal energy density of a lipid bilayer is highlighted.Considering a two-phase vesicle as system of coexisting spherical domains, its equilibrium is studied using a simple continuum model. Multidomain and ground-state configurations are considered. Whereas in the former case multiple budded lipid domains coexist on a vesicle, in the latter case the vesicle is composed of two large lipid domains. Variations of the net potential-energy of a multidomain vesicle with the number of lipid domains and osmotic pressure are studied. Based on an energy comparison argument, two ground-state configurations corresponding to minimum energy levels are identified: pinched-off and complete sphere configurations. The results indicate that osmotic pressure and initial excess radius play key roles in the final shape of attaining ground-state configurations. The critical values of these parameters are identified. Lastly, the equilibrium and stability of a discoidal HDL particle are studied. A model in which the lipid bilayer and double-belt apoA-I components of discoidal HDL particle are represented by a material surface and a material curve perfectly bonded to the edge of the surface is proposed. The curvature energy and surface tension of lipid bilayer and the bending energy of apoA-I chain are included. Adopting a variational scheme, nonlinear equilibrium equations of a discoidal HDL particle in a general configuration are derived using both direct, geometrically-based and parametrized formulations. The linearized equilibrium equations of a flat circular HDL particle are obtained and its linear stability is investigated using the second variation method. An energy comparison method is applied and is found to offer a handy approach for ascertaining linear stability. Numerical results are provided for the equilibrium and stability of flat circular HDL particle. A stability plane indicating different stable and unstable regions of underlying dimensionless input parameters is provided. Possible pathways of stability change and instability mode shapes are identified. It is shown that the first transverse and planar instability modes resemble nonplanar saddle-like and planar elliptic shapes, respectively.
Des méthodes de milieux continus pour la modélisation de bicouches lipidiques sont développées et appliquées à des vésicules lipidiques à deux phases et à des particules discoïdes de lipoprotéines de haute densité (HDL). Tout d'abord, en s'appuyant sur un modéle tridimensionnel, la mécanique d'une bicouche lipidique possédant une courbure spontanée est considérée. La Cinématique, la symétrie matérielle, les relations de stress, et la cohérence de bicouches lipidiques sont discutées. En traitant une bicouche lipidique comme une structure mince, la densité d'énergie surfacique d'une bicouche lipidique ayant une courbure spontanée est obtenue à l'aide d'une procédure de réduction de dimension. L'attention est portée sur la source de courbure spontanée de la densité d'énergie bien connue de Canham–Helfrich. En outre, l'effet de l'asymétrie constitutive des sur la densité d'énergie surfacique d'une bicouche lipidique est mis en évidence. Considérant une vésicule à deux phases comme système de domaines sphériques coexistants, son équilibre est étudié à l'aide d'un modèle simple de milieu continu. Des configurations multi-domaines et de l'état fondamental sont considérées. Alors que, dans le premier cas, plusieurs domaines lipidiques bourgeonnés coexistent sur une vésicule, dans le dernier cas, la vésicule est composée de deux grands domaines lipidiques. La variation de l'énergie potentielle nette d'une vésicule multi-domaine en fonction du nombre de domaines lipidiques et de la pression osmotique est étudiée. En se basant sur la comparaison de l'énergie, deux configurations de l'état fondamental correspondant à des niveaux d'énergie minimaux sont identifiés: la configuration étranglée et la sphère complète. Les résultats indiquent que la pression osmotique et le rayon excédentaire initial jouent un rôle clé dans la forme finale des configurations à l'état fondamental. Les valeurs critiques de ces paramètres sont identifiées. Enfin, l'équilibre et la stabilité d'une particule HDL discoïde sont étudiés. Un modèle dans lequel la bicouche lipidique et les composants d'ApoA-I à double bande de la particule de HDL discoïde sont représentées par une surface de matériau et une courbe de matériau parfaitement collée sur le bord de la surface est proposé. L'énergie de courbure et la tension de surface de la bicouche lipidique ainsi que l'énergie de flexion de la chaîne apoA-I sont incluses. En adoptant un schéma variationnel, les équations d'équilibre non-linéaire d'une particule de HDL discoïdale dans une configuration générale sont calculées d'après des formulations directes, basées sur la géométrie, ou paramétrées. Les équations d'équilibre linéarisées d'une particule de HDL circulaire plane sont obtenues et sa stabilité linéaire est étudiée en utilisant la seconde méthode de variation. Une méthode de comparaison de l'énergie est appliquée et se trouve à offrir une approche pratique pour déterminer la stabilité linéaire. Des résultats numériques sont présentés pour l'équilibre et la stabilité des particules de HDL circulaires planes. Un plan de stabilité indiquant différentes régions stables et instables des paramètres d'entrée adimensionnels sous-jacents est fourni. Certaines possibilités de changement de stabilité et les formes modales d'instabilité sont identifiées. Il est démontré que les premiers modes d'instabilité transversale et plane ressemblent aux formes de selle non planes et d'ellipse plane, respectivement.

APA, Harvard, Vancouver, ISO und andere Zitierweisen

Peter, Samuel N. K. „Polymerized-depolymerized Vesicles : reversible thiol-disulfide-based phosphatidylcholine membranes /“. Ann Arbor : Univ. Microfilms Intern, 1988. http://www.gbv.de/dms/bs/toc/016151828.pdf.

Der volle Inhalt der Quelle

APA, Harvard, Vancouver, ISO und andere Zitierweisen

Connell, Karen Elizabeth. „Studies of transport through curved and planar lipid bilayers /“. Title page, contents and summary only, 1990. http://web4.library.adelaide.edu.au/theses/09PH/09phc7522.pdf.

Der volle Inhalt der Quelle

APA, Harvard, Vancouver, ISO und andere Zitierweisen

Mehr Quellen

Bücher zum Thema "Membranes, bilayers and vesicles"

service), ScienceDirect (Online, Hrsg. Computational modeling of membrane bilayers. London: Academic Press, 2008.

Den vollen Inhalt der Quelle finden

APA, Harvard, Vancouver, ISO und andere Zitierweisen

Ottova-Leitmannova, Angelica, und H. Ti Tien. Advances in planar lipid bilayers and liposomes. Herausgegeben von Iglic Ales. Amsterdam: Elsevier/Academic Press, 2005.

Den vollen Inhalt der Quelle finden

APA, Harvard, Vancouver, ISO und andere Zitierweisen

Hanke, W. Planar lipid bilayers: Methods and applications. London: Academic Press, 1993.

Den vollen Inhalt der Quelle finden

APA, Harvard, Vancouver, ISO und andere Zitierweisen

Handbook of lipid bilayers. 2. Aufl. Boca Raton, FL: CRC Press, 2012.

Den vollen Inhalt der Quelle finden

APA, Harvard, Vancouver, ISO und andere Zitierweisen

Liu, A. Leitmannova. Advances in Planar Lipid Bilayers and Liposomes. San Diego: Elsevier Science & Technology Books., 2008.

Den vollen Inhalt der Quelle finden

APA, Harvard, Vancouver, ISO und andere Zitierweisen

Ottova-Leitmannova, Angelica, und Ales Iglic. Advances in planar lipid bilayers and liposomes. Amsterdam: Academic Press, 2009.

Den vollen Inhalt der Quelle finden

APA, Harvard, Vancouver, ISO und andere Zitierweisen

Marsh, Derek. CRC handbook of lipid bilayers. Boca Raton, Fla: CRC Press, 1990.

Den vollen Inhalt der Quelle finden

APA, Harvard, Vancouver, ISO und andere Zitierweisen

Derek, Marsh, Hrsg. Phospholipid bilayers: Physical principles and models. New York: Wiley, 1987.

Den vollen Inhalt der Quelle finden

APA, Harvard, Vancouver, ISO und andere Zitierweisen

Crowell, Kevin James. Solid state deuterium nuclear magnetic resonance investigation of the interaction of positively-charged polyelectrolytes with negatively-charged lipid bilayer membrane vesicles. Ottawa: National Library of Canada = Bibliothèque nationale du Canada, 1999.

Den vollen Inhalt der Quelle finden

APA, Harvard, Vancouver, ISO und andere Zitierweisen

Finkelstein, Alan. Water movement through lipid bilayers, pores, and plasma membranes: Theory and reality. New York: Wiley, 1987.

Den vollen Inhalt der Quelle finden

APA, Harvard, Vancouver, ISO und andere Zitierweisen

Mehr Quellen

Buchteile zum Thema "Membranes, bilayers and vesicles"

Silver, Brian L. „Micelles, Vesicles, and Bilayers — Steric Factors“. In The Physical Chemistry of MEMBRANES, 57–74. Dordrecht: Springer Netherlands, 1985. http://dx.doi.org/10.1007/978-94-010-9628-7_4.

Der volle Inhalt der Quelle

APA, Harvard, Vancouver, ISO und andere Zitierweisen

Klösgen, Beate. „Conformations of Fluid Lipid Membranes“. In Lipid Bilayers, 47–88. Berlin, Heidelberg: Springer Berlin Heidelberg, 2001. http://dx.doi.org/10.1007/978-3-662-04496-4_3.

Der volle Inhalt der Quelle

APA, Harvard, Vancouver, ISO und andere Zitierweisen

Silver, Brian L. „Hydrated Bilayers“. In The Physical Chemistry of MEMBRANES, 33–55. Dordrecht: Springer Netherlands, 1985. http://dx.doi.org/10.1007/978-94-010-9628-7_3.

Der volle Inhalt der Quelle

APA, Harvard, Vancouver, ISO und andere Zitierweisen

Lohmüller, Theo, Bert Nickel und Joachim O. Rädler. „Supported Lipid Bilayers“. In Handbook of Lipid Membranes, 293–304. Boca Raton: CRC Press, 2021. http://dx.doi.org/10.1201/9780429194078-16.

Der volle Inhalt der Quelle

APA, Harvard, Vancouver, ISO und andere Zitierweisen

Stegmann, Toon, Justin Teissie und Mathias Winterhalter. „Fusion and Rupture of Lipid Model Membranes“. In Lipid Bilayers, 265–87. Berlin, Heidelberg: Springer Berlin Heidelberg, 2001. http://dx.doi.org/10.1007/978-3-662-04496-4_12.

Der volle Inhalt der Quelle

APA, Harvard, Vancouver, ISO und andere Zitierweisen

Prosser, R. Scott, und Charles R. Sanders. „Solid State NMR Approaches to the Study of Membrane Proteins in Magnetically Aligned Model Membranes“. In Lipid Bilayers, 207–31. Berlin, Heidelberg: Springer Berlin Heidelberg, 2001. http://dx.doi.org/10.1007/978-3-662-04496-4_10.

Der volle Inhalt der Quelle

APA, Harvard, Vancouver, ISO und andere Zitierweisen

Auth, Thorsten, Dmitry A. Fedosov und Gerhard Gompper. „Simulating membranes, vesicles, and cells“. In The Giant Vesicle Book, 169–93. Boca Raton, FL : CRC Press, Taylor & Francis Group, [2020]: CRC Press, 2019. http://dx.doi.org/10.1201/9781315152516-6.

Der volle Inhalt der Quelle

APA, Harvard, Vancouver, ISO und andere Zitierweisen

Stouch, Terry R., und Donna Bassolino. „Movement of Small Molecules in Lipid Bilayers: Molecular Dynamics Simulation Studies“. In Biological Membranes, 255–79. Boston, MA: Birkhäuser Boston, 1996. http://dx.doi.org/10.1007/978-1-4684-8580-6_8.

Der volle Inhalt der Quelle

APA, Harvard, Vancouver, ISO und andere Zitierweisen

Kim, Jung-Keun, Chang-Soo Lee und Eunji Lee. „Smart Vesicles: Synthesis, Characterization and Applications“. In Smart Membranes and Sensors, 53–103. Hoboken, NJ, USA: John Wiley & Sons, Inc., 2014. http://dx.doi.org/10.1002/9781119028642.ch3.

Der volle Inhalt der Quelle

APA, Harvard, Vancouver, ISO und andere Zitierweisen

Cevc, G., W. Fenzl und L. Sigl. „Surface Induced Fusion of Vesicles into Planar Bilayers“. In Springer Proceedings in Physics, 162–65. Berlin, Heidelberg: Springer Berlin Heidelberg, 1992. http://dx.doi.org/10.1007/978-3-642-84763-9_31.

Der volle Inhalt der Quelle

APA, Harvard, Vancouver, ISO und andere Zitierweisen

Konferenzberichte zum Thema "Membranes, bilayers and vesicles"

Taylor, Graham, Donald Leo und Andy Sarles. „Detection of Botulinum Neurotoxin/A Insertion Using an Encapsulated Interface Bilayer“. In ASME 2012 Conference on Smart Materials, Adaptive Structures and Intelligent Systems. American Society of Mechanical Engineers, 2012. http://dx.doi.org/10.1115/smasis2012-8101.

Der volle Inhalt der Quelle

Annotation:

Many signaling mechanisms in living cells occur at biological boundaries via cell surface receptors and membrane proteins embedded in lipid bilayers. The coordination of actions of sensory and motor neurons in the nervous system represents one example of many that heavily depends on lipid membrane bound receptor mediated signaling. As a result, chemical and biological toxins that disrupt these neural signals can have severe physiological effects, including paralysis and death. Botulinum neurotoxin Type A (BoNT/A) is a proteolytic toxin that inserts through vesicle membranes and cleaves membrane receptors involved with synaptic acetylcholine uptake and nervous system signal conduction. In this work, we investigate the use of a Bioinspired liquid-supported interface bilayer for studying the insertion of BoNT/A toxin molecules into synthetic lipid bilayers. DPhPC lipid bilayers are formed using the regulated attachment method (RAM), as developed by Sarles and Leo, and we perform current measurements on membranes exposed to BoNT/A toxin to characterize activity of toxin interacting with the synthetic bilayer. Control tests without toxin present are also presented. The results of these tests show an increase in the magnitude of current through the bilayer when the toxin is included. We interpret these initial results to mean that incorporation of BoNT/A toxin at a high concentration in an interface bilayer increases the permeability of the membrane as a result of toxin molecules spanning the thickness of the bilayer.

APA, Harvard, Vancouver, ISO und andere Zitierweisen

Lu, Li, Jeffrey W. Schertzer und Paul R. Chiarot. „Synthetic Asymmetric Vesicles Built Using Microfluidic Technology at High-Throughput“. In ASME 2015 13th International Conference on Nanochannels, Microchannels, and Minichannels collocated with the ASME 2015 International Technical Conference and Exhibition on Packaging and Integration of Electronic and Photonic Microsystems. American Society of Mechanical Engineers, 2015. http://dx.doi.org/10.1115/icnmm2015-48556.

Der volle Inhalt der Quelle

Annotation:

We report on a novel microfluidic strategy for building monodisperse asymmetric vesicles with customized composition, size, and interfacial properties at high-throughput. The microfluidic device encompasses a triangular post region and two flow-focusing regions. The major steps involved in the vesicle building process include: (1) forming highly uniform water emulsion templates in the inner-leaflet lipid solution, (2) replacing the inner-leaflet lipid solution with the outer-leaflet lipid solution, (3) creating water-in-oil-in-water double emulsions, and (4) extracting the excess outer-leaflet lipid solution from the double emulsions. Bilayer membrane asymmetry and unilamellarity are confirmed using a fluorescence quenching assay and quantitative measurements of fluorescent intensities. This method addresses many of the deficiencies found in existing technologies, and yields asymmetries as high as 95%. The asymmetric vesicles built using this strategy hold the potential to serve as model systems to investigate fundamental problems in membrane biology.

APA, Harvard, Vancouver, ISO und andere Zitierweisen

Zhang, Hao, Vishnu Baba Sundaresan, Sergio Salinas und Robert Northcutt. „Electrochemical Analysis of Alamethicin Reconstituted Planar Bilayer Lipid Membranes Supported on Polypyrrole Membranes“. In ASME 2011 Conference on Smart Materials, Adaptive Structures and Intelligent Systems. ASMEDC, 2011. http://dx.doi.org/10.1115/smasis2011-5038.

Der volle Inhalt der Quelle

Annotation:

Conducting polymers possess similarity in ion transport function to cell membranes and perform electro-chemo-mechanical energy conversion. In an in vitro setup, protein-reconstituted bilayer lipid membranes (bioderived membranes)perform similar energy conversion and behave like cell membranes. Inspired by the similarity in ionic function between a conducting polymer membrane and cell membrane, this article presents a thin-film laminated membrane in which alamethicin-reconstituted lipid bilayer membrane is supported on a polypyrrole membrane. Owing to the synthetic and bioderived nature of the components of the membrane, we refer to the laminated membrane as a hybrid bioderived membrane. In this article, we describe the fabrication steps and electrochemical characterization of the hybrid membrane. The fabrication steps include electropolymerization of pyrrole and vesicle fusion to result in a hybrid membrane; and the characterization involves electrical impedance spectroscopy, chronoamperometry and cyclic voltammetry. The resistance and capacitance of BLM have the magnitude of 4.6×109Ω-cm2 and 1.6×10−8 F/cm2.The conductance of alamethicin has the magnitude of 6.4×10−8 S/cm2. The change in ionic conductance of the bioderived membrane is due to the electrical field applied across alamethicin, a voltage-gated protein and produces a measurable change in the ionic concentration of the conducting polymer substrate.

APA, Harvard, Vancouver, ISO und andere Zitierweisen

Ahmed, Tanzir, Sander van den Driesche, Jayesh Arun Bafna, Martin Oellers, Roland Hemmler, Karsten Gall, Richard Wagner, Mathias Winterhalter und Michael J. Vellekoop. „Parylene-C coated micro-apertures with painted synthetic lipid bilayer membranes for the investigation of outer-membrane-vesicle fusion“. In 2019 IEEE SENSORS. IEEE, 2019. http://dx.doi.org/10.1109/sensors43011.2019.8956698.

Der volle Inhalt der Quelle

APA, Harvard, Vancouver, ISO und andere Zitierweisen

Zhu, Qiang, Zhangli Peng und Robert J. Asaro. „Investigation of RBC Remodeling With a Multiscale Model“. In ASME 2010 First Global Congress on NanoEngineering for Medicine and Biology. ASMEDC, 2010. http://dx.doi.org/10.1115/nemb2010-13121.

Der volle Inhalt der Quelle

Annotation:

Erythrocyte (red blood cell, or RBC) possesses one of the simplest and best characterized molecular architectures among all cells. It contains cytosol enclosed inside a composite membrane consisting of a fluidic lipid bilayer reinforced by a single layer of protein skeleton pinned to it. In its normal state, this system demonstrates tremendous structural stability, manifested in its ability to sustain large dynamic deformations during circulation. On the other hand, it has been illustrated in experiments that triggered by mechanical loads structural remodeling may occur. A canonical example of this remodeling is vesiculation, referring to the partial separation of the lipid bilayer from the protein skeleton and the formation of vesicles that contain lipids only.

APA, Harvard, Vancouver, ISO und andere Zitierweisen

Creasy, M. Austin, und Donald J. Leo. „Non-Invasive Measurement Techniques for Measuring Bilayers in Droplet-Interface-Bilayers“. In ASME 2009 Conference on Smart Materials, Adaptive Structures and Intelligent Systems. ASMEDC, 2009. http://dx.doi.org/10.1115/smasis2009-1321.

Der volle Inhalt der Quelle

Annotation:

Phospholipids and membrane proteins are two of the fundamental building blocks of cell membranes in living organisms. These molecules are amphipathic and synthetic membranes made of phospholipids, called bilayer lipid membranes (BLMs), are used to understand the characteristics of a cell membrane. Studies of these BLMs have been performed on both solid support and liquid support systems. A droplet interface bilayer (DIB) is a liquid support system where a monolayer is formed around a water droplet placed in oil and a bilayer is formed when two of these droplets are placed in contact. For impedance measurements, electrodes are placed in the water on each side of the bilayer. The measurements first insure there is a bilayer and second to obtain information about the bilayer. In a DIB system the electrodes pierce the monolayer surrounding the droplet causing instabilities in the monolayer. This study focuses on a non-invasive technique for measuring the bilayer by using the electrodes to contact the monolayer around the droplets and take impedance measurements without piercing the monolayer.

APA, Harvard, Vancouver, ISO und andere Zitierweisen

Md Jani, Abdul M., Jinwen Zhou, Matthew R. Nussio, Dusan Losic, Joe G. Shapter und Nicolas H. Voelcker. „Pore spanning lipid bilayers on silanised nanoporous alumina membranes“. In Smart Materials, Nano-and Micro-Smart Systems, herausgegeben von Nicolas H. Voelcker und Helmut W. Thissen. SPIE, 2008. http://dx.doi.org/10.1117/12.808769.

Der volle Inhalt der Quelle

APA, Harvard, Vancouver, ISO und andere Zitierweisen

Thaokar, Rochish M. „Instability of Charged Planar Membranes and Spherical and Cylindrical Vesicles“. In 14th Asia Pacific Confederation of Chemical Engineering Congress. Singapore: Research Publishing Services, 2012. http://dx.doi.org/10.3850/978-981-07-1445-1_517.

Der volle Inhalt der Quelle

APA, Harvard, Vancouver, ISO und andere Zitierweisen

Basham, Colin, Megan Pitz, Joseph Najem, Stephen Sarles und Md Sakib Hasan. „Memcapacitive Devices in Neuromorphic Circuits via Polymeric Biomimetic Membranes“. In ASME 2019 Conference on Smart Materials, Adaptive Structures and Intelligent Systems. American Society of Mechanical Engineers, 2019. http://dx.doi.org/10.1115/smasis2019-5648.

Der volle Inhalt der Quelle

Annotation:

Abstract Two-terminal adaptive materials and circuit elements that mimic the signal processing, learning, and computing capabilities of biological synapses are essential for next-generation computing systems. To this end, we have recently developed resistive (ion channel) and capacitive (lipid bilayer) memory elements that mimic the composition, structure, and plasticity of biological synapses. Unlike solid-state counterparts, these biomolecular systems are low-power, analog, less noisy, biocompatible, and capable of exhibiting multiple timescales of short-term synaptic plasticity. However, lipid membranes lack structural stability and modularity necessary for a long-lasting adaptive material system. To address this issue, we propose the replacement of phospholipids with amphiphilic polymers to create artificial membranes, which have been demonstrated to be more durable than phospholipids. With the focus on memory capacitors, we demonstrate that polymeric bilayers can exhibit pinched hysteresis in the Q-v plane because of voltage-induced geometrical changes. Further, we demonstrate that the memcapacitive response is altered based on the surrounding oil medium; smaller oil molecules are retained at higher volume in the membrane, so that thicker bilayers have lower nominal capacitance but can vary this value by over 400%. Finally, we present a physics-based model that enables us to predict the device’s areal voltage-dependent response. Polymeric bilayers represent a significant enhancement in the field of soft-matter, geometrically-reconfigurable memcapacitors, and their highly customizable compositions will allow for a finely tuned electrical response that has a future in brain-inspired materials and circuits.

APA, Harvard, Vancouver, ISO und andere Zitierweisen

Lee, Hyeseung, Dean Ho, Benjamin Chu, Karen Kuo und Carlo Montemagno. „Reconstituting Membrane Proteins Into Artificial Membranes and Detection of Their Activities“. In ASME 2004 3rd Integrated Nanosystems Conference. ASMEDC, 2004. http://dx.doi.org/10.1115/nano2004-46016.

Der volle Inhalt der Quelle

Annotation:

We have successfully purified BR from purple membrane of Halobacterium Salinarium and Cox from the genetically engineered plasmid inserted in Rhodobacter Sphaeroides. The activities of the purified enzymes have shown in lipid vesicles as well as in polymer vesicles and planar membranes. Phosphatidylcholine derived lipid vesicles created the most nature like environment for the enzymes. Triblock copolymer membrane was the alternative choice for membrane protein reconstitution since polymers are more durable, ideal for industrial applications and support enzyme activities better. We also demonstrated the backward function of Cox in vitro by changing proton concentration in the surrounding medium. Langmuir-Blodgett method was used to reconstitute the enzymes into the planar lipid or polymer membranes. The enzyme activities of the enzymes in planar membrane system were tested by impedance spectroscopy.

APA, Harvard, Vancouver, ISO und andere Zitierweisen

Wir bieten Rabatte auf alle Premium-Pläne für Autoren, deren Werke in thematische Literatursammlungen aufgenommen wurden. Kontaktieren Sie uns, um einen einzigartigen Promo-Code zu erhalten!