Einloggen

Thematische Bibliographien / Mitotic spindle orientations / Zeitschriftenartikel

Um die anderen Arten von Veröffentlichungen zu diesem Thema anzuzeigen, folgen Sie diesem Link: Mitotic spindle orientations.

Zeitschriftenartikel zum Thema „Mitotic spindle orientations“

Autor: Grafiati

Veröffentlicht am 25. Mai 2024

Geben Sie eine Quelle nach APA, MLA, Chicago, Harvard und anderen Zitierweisen an

Wählen Sie eine Art der Quelle aus:

Machen Sie sich mit Top-50 Zeitschriftenartikel für die Forschung zum Thema "Mitotic spindle orientations" bekannt.

Neben jedem Werk im Literaturverzeichnis ist die Option "Zur Bibliographie hinzufügen" verfügbar. Nutzen Sie sie, wird Ihre bibliographische Angabe des gewählten Werkes nach der nötigen Zitierweise (APA, MLA, Harvard, Chicago, Vancouver usw.) automatisch gestaltet.

Sie können auch den vollen Text der wissenschaftlichen Publikation im PDF-Format herunterladen und eine Online-Annotation der Arbeit lesen, wenn die relevanten Parameter in den Metadaten verfügbar sind.

Sehen Sie die Zeitschriftenartikel für verschiedene Spezialgebieten durch und erstellen Sie Ihre Bibliographie auf korrekte Weise.

1

Wang, S. W., F. J. Griffin, and W. H. Clark. "Cell-cell association directed mitotic spindle orientation in the early development of the marine shrimp Sicyonia ingentis." Development 124, no. 4 (1997): 773–80. http://dx.doi.org/10.1242/dev.124.4.773.

Der volle Inhalt der Quelle

Annotation:

During early cleavages of Sicyonia ingentis embryos, mitotic spindle orientations differ between blastomeres and change in a predictable manner with each successive mitosis. From 2nd through 7th cleavages, spindles orient at a 90 degrees angle with respect to the spindle of the parent blastomere. Thus, spindle orientation is parallel to the cleavage plane that formed the blastomere. To determine if specific spindle orientations were intrinsic properties of individual blastomeres, we altered blastomere associations and asked how mitotic spindle orientation was affected in successive cleavages u

APA, Harvard, Vancouver, ISO und andere Zitierweisen

2

Juschke, C., Y. Xie, M. P. Postiglione, and J. A. Knoblich. "Analysis and modeling of mitotic spindle orientations in three dimensions." Proceedings of the National Academy of Sciences 111, no. 3 (2013): 1014–19. http://dx.doi.org/10.1073/pnas.1314984111.

Der volle Inhalt der Quelle

APA, Harvard, Vancouver, ISO und andere Zitierweisen

3

Chan, Derek C. H., Joshua Xu, Ana Vujovic, et al. "Arhgef2 regulates mitotic spindle orientation in hematopoietic stem cells and is essential for productive hematopoiesis." Blood Advances 5, no. 16 (2021): 3120–33. http://dx.doi.org/10.1182/bloodadvances.2020002539.

Der volle Inhalt der Quelle

Annotation:

Abstract How hematopoietic stem cells (HSCs) coordinate their divisional axis and whether this orientation is important for stem cell–driven hematopoiesis is poorly understood. Single-cell RNA sequencing data from patients with Shwachman-Diamond syndrome (SDS), an inherited bone marrow failure syndrome, show that ARHGEF2, a RhoA-specific guanine nucleotide exchange factor and determinant of mitotic spindle orientation, is specifically downregulated in SDS hematopoietic stem and progenitor cells (HSPCs). We demonstrate that transplanted Arhgef2−/− fetal liver and bone marrow cells yield impaire

APA, Harvard, Vancouver, ISO und andere Zitierweisen

4

Sei, Yoshitatsu, Jianying Feng, Carson C. Chow, and Stephen A. Wank. "Asymmetric cell division-dominant neutral drift model for normal intestinal stem cell homeostasis." American Journal of Physiology-Gastrointestinal and Liver Physiology 316, no. 1 (2019): G64—G74. http://dx.doi.org/10.1152/ajpgi.00242.2018.

Der volle Inhalt der Quelle

Annotation:

The normal intestinal epithelium is continuously regenerated at a rapid rate from actively cycling Lgr5-expressing intestinal stem cells (ISCs) that reside at the crypt base. Recent mathematical modeling based on several lineage-tracing studies in mice shows that the symmetric cell division-dominant neutral drift model fits well with the observed in vivo growth of ISC clones and suggests that symmetric divisions are central to ISC homeostasis. However, other studies suggest a critical role for asymmetric cell division in the maintenance of ISC homeostasis in vivo. Here, we show that the stocha

APA, Harvard, Vancouver, ISO und andere Zitierweisen

5

Copp, A. J., F. A. Brook, and H. J. Roberts. "A cell-type-specific abnormality of cell proliferation in mutant (curly tail) mouse embryos developing spinal neural tube defects." Development 104, no. 2 (1988): 285–95. http://dx.doi.org/10.1242/dev.104.2.285.

Der volle Inhalt der Quelle

Annotation:

The mouse mutant curly tail (ct) provides a model system for studies of neurulation mechanisms. 60% of ct/ct embryos develop spinal neural tube defects (NTD) as a result of delayed neurulation at the posterior neuropore whereas the remaining 40% of embryos develop normally. In order to investigate the role of cell proliferation during mouse neurulation, cell cycle parameters were studied in curly tail embryos developing spinal NTD and in their normally developing litter-mates. Measurements were made of mitotic index, median length of S-phase and percent reduction of labelling index during a [3

APA, Harvard, Vancouver, ISO und andere Zitierweisen

6

Li, Jie, Hiroki Shima, Hironari Nishizawa, et al. "Phosphorylation of BACH1 switches its function from transcription factor to mitotic chromosome regulator and promotes its interaction with HMMR." Biochemical Journal 475, no. 5 (2018): 981–1002. http://dx.doi.org/10.1042/bcj20170520.

Der volle Inhalt der Quelle

Annotation:

The transcription repressor BACH1 performs mutually independent dual roles in transcription regulation and chromosome alignment during mitosis by supporting polar ejection force of mitotic spindle. We now found that the mitotic spindles became oblique relative to the adhesion surface following endogenous BACH1 depletion in HeLa cells. This spindle orientation rearrangement was rescued by re-expression of BACH1 depending on its interactions with HMMR and CRM1, both of which are required for the positioning of mitotic spindle, but independently of its DNA-binding activity. A mass spectrometry an

APA, Harvard, Vancouver, ISO und andere Zitierweisen

7

Kapoor, Tarun M., Thomas U. Mayer, Margaret L. Coughlin, and Timothy J. Mitchison. "Probing Spindle Assembly Mechanisms with Monastrol, a Small Molecule Inhibitor of the Mitotic Kinesin, Eg5." Journal of Cell Biology 150, no. 5 (2000): 975–88. http://dx.doi.org/10.1083/jcb.150.5.975.

Der volle Inhalt der Quelle

Annotation:

Monastrol, a cell-permeable small molecule inhibitor of the mitotic kinesin, Eg5, arrests cells in mitosis with monoastral spindles. Here, we use monastrol to probe mitotic mechanisms. We find that monastrol does not inhibit progression through S and G2 phases of the cell cycle or centrosome duplication. The mitotic arrest due to monastrol is also rapidly reversible. Chromosomes in monastrol-treated cells frequently have both sister kinetochores attached to microtubules extending to the center of the monoaster (syntelic orientation). Mitotic arrest–deficient protein 2 (Mad2) localizes to a sub

APA, Harvard, Vancouver, ISO und andere Zitierweisen

8

Woodard, Geoffrey E., Ning-Na Huang, Hyeseon Cho, Toru Miki, Gregory G. Tall та John H. Kehrl. "Ric-8A and Giα Recruit LGN, NuMA, and Dynein to the Cell Cortex To Help Orient the Mitotic Spindle". Molecular and Cellular Biology 30, № 14 (2010): 3519–30. http://dx.doi.org/10.1128/mcb.00394-10.

Der volle Inhalt der Quelle

Annotation:

ABSTRACT In model organisms, resistance to inhibitors of cholinesterase 8 (Ric-8), a G protein α (Gα) subunit guanine nucleotide exchange factor (GEF), functions to orient mitotic spindles during asymmetric cell divisions; however, whether Ric-8A has any role in mammalian cell division is unknown. We show here that Ric-8A and Gαi function to orient the metaphase mitotic spindle of mammalian adherent cells. During mitosis, Ric-8A localized at the cell cortex, spindle poles, centromeres, central spindle, and midbody. Pertussis toxin proved to be a useful tool in these studies since it blocked th

APA, Harvard, Vancouver, ISO und andere Zitierweisen

9

Siletti, Kimberly, Basile Tarchini, and A. J. Hudspeth. "Daple coordinates organ-wide and cell-intrinsic polarity to pattern inner-ear hair bundles." Proceedings of the National Academy of Sciences 114, no. 52 (2017): E11170—E11179. http://dx.doi.org/10.1073/pnas.1716522115.

Der volle Inhalt der Quelle

Annotation:

The establishment of planar polarization by mammalian cells necessitates the integration of diverse signaling pathways. In the inner ear, at least two systems regulate the planar polarity of sensory hair bundles. The core planar cell polarity (PCP) proteins coordinate the orientations of hair cells across the epithelial plane. The cell-intrinsic patterning of hair bundles is implemented independently by the G protein complex classically known for orienting the mitotic spindle. Although the primary cilium also participates in each of these pathways, its role and the integration of the two syste

APA, Harvard, Vancouver, ISO und andere Zitierweisen

10

Giansanti, M. G., M. Gatti, and S. Bonaccorsi. "The role of centrosomes and astral microtubules during asymmetric division of Drosophila neuroblasts." Development 128, no. 7 (2001): 1137–45. http://dx.doi.org/10.1242/dev.128.7.1137.

Der volle Inhalt der Quelle

Annotation:

Drosophila neuroblasts are stem cells that divide asymmetrically to produce another large neuroblast and a smaller ganglion mother cell (GMC). During neuroblast division, several cell fate determinants, such as Miranda, Prospero and Numb, are preferentially segregated into the GMC, ensuring its correct developmental fate. The accurate segregation of these determinants relies on proper orientation of the mitotic spindle within the dividing neuroblast, and on the correct positioning of the cleavage plane. In this study we have analyzed the role of centrosomes and astral microtubules in neuroblas

APA, Harvard, Vancouver, ISO und andere Zitierweisen

11

Li, Jingchen, Longcan Cheng, and Hongyuan Jiang. "Cell shape and intercellular adhesion regulate mitotic spindle orientation." Molecular Biology of the Cell 30, no. 19 (2019): 2458–68. http://dx.doi.org/10.1091/mbc.e19-04-0227.

Der volle Inhalt der Quelle

Annotation:

Cell division orientation plays an essential role in tissue morphogenesis and cell fate decision. Recent studies showed that either cell shape or adhesion geometry can regulate the orientation of mitotic spindles and thereby the cell division orientation. However, how they together regulate the spindle orientation remains largely unclear. In this work, we use a general computational model to investigate the competitive mechanism of determining the spindle orientation between cell shape and intercellular adhesion in epithelial cells. We find the spindle orientation is dominated by the intercell

APA, Harvard, Vancouver, ISO und andere Zitierweisen

12

Gassmann, Reto, Ana Carvalho, Alexander J. Henzing, et al. "Borealin." Journal of Cell Biology 166, no. 2 (2004): 179–91. http://dx.doi.org/10.1083/jcb.200404001.

Der volle Inhalt der Quelle

Annotation:

The chromosomal passenger complex of Aurora B kinase, INCENP, and Survivin has essential regulatory roles at centromeres and the central spindle in mitosis. Here, we describe Borealin, a novel member of the complex. Approximately half of Aurora B in mitotic cells is complexed with INCENP, Borealin, and Survivin; and Borealin binds Survivin and INCENP in vitro. A second complex contains Aurora B and INCENP, but no Borealin or Survivin. Depletion of Borealin by RNA interference delays mitotic progression and results in kinetochore–spindle misattachments and an increase in bipolar spindles associ

APA, Harvard, Vancouver, ISO und andere Zitierweisen

13

Chan, Ying Wai, Luca L. Fava, Andreas Uldschmid, et al. "Mitotic control of kinetochore-associated dynein and spindle orientation by human Spindly." Journal of Cell Biology 185, no. 5 (2009): 859–74. http://dx.doi.org/10.1083/jcb.200812167.

Der volle Inhalt der Quelle

Annotation:

Mitotic spindle formation and chromosome segregation depend critically on kinetochore–microtubule (KT–MT) interactions. A new protein, termed Spindly in Drosophila and SPDL-1 in C. elegans, was recently shown to regulate KT localization of dynein, but depletion phenotypes revealed striking differences, suggesting evolutionarily diverse roles of mitotic dynein. By characterizing the function of Spindly in human cells, we identify specific functions for KT dynein. We show that localization of human Spindly (hSpindly) to KTs is controlled by the Rod/Zw10/Zwilch (RZZ) complex and Aurora B. hSpindl

APA, Harvard, Vancouver, ISO und andere Zitierweisen

14

Larson, Matthew E., and William M. Bement. "Automated mitotic spindle tracking suggests a link between spindle dynamics, spindle orientation, and anaphase onset in epithelial cells." Molecular Biology of the Cell 28, no. 6 (2017): 746–59. http://dx.doi.org/10.1091/mbc.e16-06-0355.

Der volle Inhalt der Quelle

Annotation:

Proper spindle positioning at anaphase onset is essential for normal tissue organization and function. Here we develop automated spindle-tracking software and apply it to characterize mitotic spindle dynamics in the Xenopus laevis embryonic epithelium. We find that metaphase spindles first undergo a sustained rotation that brings them on-axis with their final orientation. This sustained rotation is followed by a set of striking stereotyped rotational oscillations that bring the spindle into near contact with the cortex and then move it rapidly away from the cortex. These oscillations begin to

APA, Harvard, Vancouver, ISO und andere Zitierweisen

15

Buttrick, Graham J., Luke M. A. Beaumont, Jessica Leitch, Christopher Yau, Julian R. Hughes, and James G. Wakefield. "Akt regulates centrosome migration and spindle orientation in the early Drosophila melanogaster embryo." Journal of Cell Biology 180, no. 3 (2008): 537–48. http://dx.doi.org/10.1083/jcb.200705085.

Der volle Inhalt der Quelle

Annotation:

Correct positioning and morphology of the mitotic spindle is achieved through regulating the interaction between microtubules (MTs) and cortical actin. Here we find that, in the Drosophila melanogaster early embryo, reduced levels of the protein kinase Akt result in incomplete centrosome migration around cortical nuclei, bent mitotic spindles, and loss of nuclei into the interior of the embryo. We show that Akt is enriched at the embryonic cortex and is required for phosphorylation of the glycogen synthase kinase-3β homologue Zeste-white 3 kinase (Zw3) and for the cortical localizations of the

APA, Harvard, Vancouver, ISO und andere Zitierweisen

16

Sana, Shrividya, Riya Keshri, Ashwathi Rajeevan, Sukriti Kapoor, and Sachin Kotak. "Plk1 regulates spindle orientation by phosphorylating NuMA in human cells." Life Science Alliance 1, no. 6 (2018): e201800223. http://dx.doi.org/10.26508/lsa.201800223.

Der volle Inhalt der Quelle

Annotation:

Proper orientation of the mitotic spindle defines the correct division plane and is essential for accurate cell division and development. In metazoans, an evolutionarily conserved complex comprising of NuMA/LGN/Gαi regulates proper orientation of the mitotic spindle by orchestrating cortical dynein levels during metaphase. However, the molecular mechanisms that modulate the spatiotemporal dynamics of this complex during mitosis remain elusive. Here, we report that acute inactivation of Polo-like kinase 1 (Plk1) during metaphase enriches cortical levels of dynein/NuMA/LGN and thus influences sp

APA, Harvard, Vancouver, ISO und andere Zitierweisen

17

Nakajima, Yu-ichiro, Zachary T. Lee, Sean A. McKinney, Selene K. Swanson, Laurence Florens, and Matthew C. Gibson. "Junctional tumor suppressors interact with 14-3-3 proteins to control planar spindle alignment." Journal of Cell Biology 218, no. 6 (2019): 1824–38. http://dx.doi.org/10.1083/jcb.201803116.

Der volle Inhalt der Quelle

Annotation:

Proper orientation of the mitotic spindle is essential for cell fate determination, tissue morphogenesis, and homeostasis. During epithelial proliferation, planar spindle alignment ensures the maintenance of polarized tissue architecture, and aberrant spindle orientation can disrupt epithelial integrity. Nevertheless, in vivo mechanisms that restrict the mitotic spindle to the plane of the epithelium remain poorly understood. Here we show that the junction-localized tumor suppressors Scribbled (Scrib) and Discs large (Dlg) control planar spindle orientation via Mud and 14-3-3 proteins in the D

APA, Harvard, Vancouver, ISO und andere Zitierweisen

18

Bergstralh, Dan T., Timm Haack, and Daniel St Johnston. "Epithelial polarity and spindle orientation: intersecting pathways." Philosophical Transactions of the Royal Society B: Biological Sciences 368, no. 1629 (2013): 20130291. http://dx.doi.org/10.1098/rstb.2013.0291.

Der volle Inhalt der Quelle

Annotation:

During asymmetric stem cell divisions, the mitotic spindle must be correctly oriented and positioned with respect to the axis of cell polarity to ensure that cell fate determinants are appropriately segregated into only one daughter cell. By contrast, epithelial cells divide symmetrically and orient their mitotic spindles perpendicular to the main apical–basal polarity axis, so that both daughter cells remain within the epithelium. Work in the past 20 years has defined a core ternary complex consisting of Pins, Mud and Gαi that participates in spindle orientation in both asymmetric and symmetr

APA, Harvard, Vancouver, ISO und andere Zitierweisen

19

den Elzen, Nicole, Carmen V. Buttery, Madhavi P. Maddugoda, Gang Ren, and Alpha S. Yap. "Cadherin Adhesion Receptors Orient the Mitotic Spindle during Symmetric Cell Division in Mammalian Epithelia." Molecular Biology of the Cell 20, no. 16 (2009): 3740–50. http://dx.doi.org/10.1091/mbc.e09-01-0023.

Der volle Inhalt der Quelle

Annotation:

Oriented cell division is a fundamental determinant of tissue organization. Simple epithelia divide symmetrically in the plane of the monolayer to preserve organ structure during epithelial morphogenesis and tissue turnover. For this to occur, mitotic spindles must be stringently oriented in the Z-axis, thereby establishing the perpendicular division plane between daughter cells. Spatial cues are thought to play important roles in spindle orientation, notably during asymmetric cell division. The molecular nature of the cortical cues that guide the spindle during symmetric cell division, howeve

APA, Harvard, Vancouver, ISO und andere Zitierweisen

20

O'Connell, Christopher B., and Yu-li Wang. "Mammalian Spindle Orientation and Position Respond to Changes in Cell Shape in a Dynein-dependent Fashion." Molecular Biology of the Cell 11, no. 5 (2000): 1765–74. http://dx.doi.org/10.1091/mbc.11.5.1765.

Der volle Inhalt der Quelle

Annotation:

In animal cells, positioning of the mitotic spindle is crucial for defining the plane of cytokinesis and the size ratio of daughter cells. We have characterized this phenomenon in a rat epithelial cell line using microscopy, micromanipulation, and microinjection. Unmanipulated cells position the mitotic spindle near their geometric center, with the spindle axis lying roughly parallel to the long axis of the cell. Spindles that were initially misoriented underwent directed rotation and caused a delay in anaphase onset. To gain further insight into this process, we gently deformed cells with a b

APA, Harvard, Vancouver, ISO und andere Zitierweisen

21

Lázaro-Diéguez, Francisco, Iaroslav Ispolatov, and Anne Müsch. "Cell shape impacts on the positioning of the mitotic spindle with respect to the substratum." Molecular Biology of the Cell 26, no. 7 (2015): 1286–95. http://dx.doi.org/10.1091/mbc.e14-08-1330.

Der volle Inhalt der Quelle

Annotation:

All known mechanisms of mitotic spindle orientation rely on astral microtubules. We report that even in the absence of astral microtubules, metaphase spindles in MDCK and HeLa cells are not randomly positioned along their x-z dimension, but preferentially adopt shallow β angles between spindle pole axis and substratum. The nonrandom spindle positioning is due to constraints imposed by the cell cortex in flat cells that drive spindles that are longer and/or wider than the cell's height into a tilted, quasidiagonal x-z position. In rounder cells, which are taller, fewer cortical constraints make

APA, Harvard, Vancouver, ISO und andere Zitierweisen

22

Wang, Mengqiao, and Ruth N. Collins. "A lysine deacetylase Hos3 is targeted to the bud neck and involved in the spindle position checkpoint." Molecular Biology of the Cell 25, no. 18 (2014): 2720–34. http://dx.doi.org/10.1091/mbc.e13-10-0619.

Der volle Inhalt der Quelle

Annotation:

An increasing number of cellular activities can be regulated by reversible lysine acetylation. Targeting the enzymes responsible for such posttranslational modifications is instrumental in defining their substrates and functions in vivo. Here we show that a Saccharomyces cerevisiae lysine deacetylase, Hos3, is asymmetrically targeted to the daughter side of the bud neck and to the daughter spindle pole body (SPB). The morphogenesis checkpoint member Hsl7 recruits Hos3 to the neck region. Cells with a defect in spindle orientation trigger Hos3 to load onto both SPBs. When associated symmetrical

APA, Harvard, Vancouver, ISO und andere Zitierweisen

23

Lázaro-Diéguez, Francisco, and Anne Müsch. "Cell–cell adhesion accounts for the different orientation of columnar and hepatocytic cell divisions." Journal of Cell Biology 216, no. 11 (2017): 3847–59. http://dx.doi.org/10.1083/jcb.201608065.

Der volle Inhalt der Quelle

Annotation:

Mitotic spindle alignment with the basal or substrate-contacting domain ensures that dividing epithelial cells remain in the plane of the monolayer. Spindle orientation with respect to the substratum is established in metaphase coincident with maximal cell rounding, which enables unobstructed spindle rotation. Misaligned metaphase spindles are believed to result in divisions in which one daughter loses contact with the basal lamina. Here we describe a rescue mechanism that drives substrate-parallel spindle alignment of quasi-diagonal metaphase spindles in anaphase. It requires a Rho- and E-cad

APA, Harvard, Vancouver, ISO und andere Zitierweisen

24

Takeda, Yutaka, Kaho Yamazaki, Kaho Hashimoto, Koki Watanabe, Takumi Chinen, and Daiju Kitagawa. "The centriole protein CEP76 negatively regulates PLK1 activity in the cytoplasm for proper mitotic progression." Journal of Cell Science 133, no. 19 (2020): jcs241281. http://dx.doi.org/10.1242/jcs.241281.

Der volle Inhalt der Quelle

Annotation:

ABSTRACTPolo-like kinase 1 (PLK1) dynamically changes its localization and plays important roles in proper mitotic progression. In particular, strict control of cytoplasmic PLK1 is needed to prevent mitotic defects. However, the regulation of cytoplasmic PLK1 is not fully understood. In this study, we show that CEP76, a centriolar protein, physically interacts with PLK1 and tightly controls the activation of cytoplasmic PLK1 during mitosis in human cells. We found that removal of centrosomes induced ectopic aggregation of PLK1, which is highly phosphorylated, in the cytoplasm during mitosis. I

APA, Harvard, Vancouver, ISO und andere Zitierweisen

25

Saadaoui, Mehdi, Mickaël Machicoane, Florencia di Pietro, Fred Etoc, Arnaud Echard, and Xavier Morin. "Dlg1 controls planar spindle orientation in the neuroepithelium through direct interaction with LGN." Journal of Cell Biology 206, no. 6 (2014): 707–17. http://dx.doi.org/10.1083/jcb.201405060.

Der volle Inhalt der Quelle

Annotation:

Oriented cell divisions are necessary for the development of epithelial structures. Mitotic spindle orientation requires the precise localization of force generators at the cell cortex via the evolutionarily conserved LGN complex. However, polarity cues acting upstream of this complex in vivo in the vertebrate epithelia remain unknown. In this paper, we show that Dlg1 is localized at the basolateral cell cortex during mitosis and is necessary for planar spindle orientation in the chick neuroepithelium. Live imaging revealed that Dlg1 is required for directed spindle movements during metaphase.

APA, Harvard, Vancouver, ISO und andere Zitierweisen

26

Penisson, Maxime, Mingyue Jin, Shengming Wang, Shinji Hirotsune, Fiona Francis, and Richard Belvindrah. "Lis1 mutation prevents basal radial glia-like cell production in the mouse." Human Molecular Genetics 31, no. 6 (2021): 942–57. http://dx.doi.org/10.1093/hmg/ddab295.

Der volle Inhalt der Quelle

Annotation:

Abstract Human cerebral cortical malformations are associated with progenitor proliferation and neuronal migration abnormalities. Progenitor cells include apical radial glia, intermediate progenitors and basal (or outer) radial glia (bRGs or oRGs). bRGs are few in number in lissencephalic species (e.g. the mouse) but abundant in gyrencephalic brains. The LIS1 gene coding for a dynein regulator, is mutated in human lissencephaly, associated also in some cases with microcephaly. LIS1 was shown to be important during cell division and neuronal migration. Here, we generated bRG-like cells in the m

APA, Harvard, Vancouver, ISO und andere Zitierweisen

27

Carminati, Janet L., and Tim Stearns. "Microtubules Orient the Mitotic Spindle in Yeast through Dynein-dependent Interactions with the Cell Cortex." Journal of Cell Biology 138, no. 3 (1997): 629–41. http://dx.doi.org/10.1083/jcb.138.3.629.

Der volle Inhalt der Quelle

Annotation:

Proper orientation of the mitotic spindle is critical for successful cell division in budding yeast. To investigate the mechanism of spindle orientation, we used a green fluorescent protein (GFP)–tubulin fusion protein to observe microtubules in living yeast cells. GFP–tubulin is incorporated into microtubules, allowing visualization of both cytoplasmic and spindle microtubules, and does not interfere with normal microtubule function. Microtubules in yeast cells exhibit dynamic instability, although they grow and shrink more slowly than microtubules in animal cells. The dynamic properties of y

APA, Harvard, Vancouver, ISO und andere Zitierweisen

28

Dewey, Evan B., and Christopher A. Johnston. "Diverse mitotic functions of the cytoskeletal cross-linking protein Shortstop suggest a role in Dynein/Dynactin activity." Molecular Biology of the Cell 28, no. 19 (2017): 2555–68. http://dx.doi.org/10.1091/mbc.e17-04-0219.

Der volle Inhalt der Quelle

Annotation:

Proper assembly and orientation of the bipolar mitotic spindle is critical to the fidelity of cell division. Mitotic precision fundamentally contributes to cell fate specification, tissue development and homeostasis, and chromosome distribution within daughter cells. Defects in these events are thought to contribute to several human diseases. The underlying mechanisms that function in spindle morphogenesis and positioning remain incompletely defined, however. Here we describe diverse roles for the actin-microtubule cross-linker Shortstop (Shot) in mitotic spindle function in Drosophila. Shot l

APA, Harvard, Vancouver, ISO und andere Zitierweisen

29

Loginova, Dina B., Anastasia A. Zhuravleva, and Olga G. Silkova. "Random chromosome distribution in the first meiosis of F1 disomic substitution line 2R(2D) x rye hybrids (ABDR, 4× = 28) occurs without bipolar spindle assembly." Comparative Cytogenetics 14, no. 4 (2020): 453–82. http://dx.doi.org/10.3897/compcytogen.v14.i4.55827.

Der volle Inhalt der Quelle

Annotation:

The assembly of the microtubule-based spindle structure in plant meiosis remains poorly understood compared with our knowledge of mitotic spindle formation. One of the approaches in our understanding of microtubule dynamics is to study spindle assembly in meiosis of amphyhaploids. Using immunostaining with phH3Ser10, CENH3 and α-tubulin-specific antibodies, we studied the chromosome distribution and spindle organisation in meiosis of F1 2R(2D)xR wheat-rye hybrids (genome structure ABDR, 4× = 28), as well as in wheat and rye mitosis and meiosis. At the prometaphase of mitosis, spindle assembly

APA, Harvard, Vancouver, ISO und andere Zitierweisen

30

Lee, Laifong, Saskia K. Klee, Marie Evangelista, Charles Boone, and David Pellman. "Control of Mitotic Spindle Position by the Saccharomyces cerevisiae Formin Bni1p." Journal of Cell Biology 144, no. 5 (1999): 947–61. http://dx.doi.org/10.1083/jcb.144.5.947.

Der volle Inhalt der Quelle

Annotation:

Alignment of the mitotic spindle with the axis of cell division is an essential process in Saccharomyces cerevisiae that is mediated by interactions between cytoplasmic microtubules and the cell cortex. We found that a cortical protein, the yeast formin Bni1p, was required for spindle orientation. Two striking abnormalities were observed in bni1Δ cells. First, the initial movement of the spindle pole body (SPB) toward the emerging bud was defective. This phenotype is similar to that previously observed in cells lacking the kinesin Kip3p and, in fact, BNI1 and KIP3 were found to be in the same

APA, Harvard, Vancouver, ISO und andere Zitierweisen

31

Chippalkatti, Rohan, Boris Egger, and Beat Suter. "Mms19 promotes spindle microtubule assembly in Drosophila neural stem cells." PLOS Genetics 16, no. 11 (2020): e1008913. http://dx.doi.org/10.1371/journal.pgen.1008913.

Der volle Inhalt der Quelle

Annotation:

Mitotic divisions depend on the timely assembly and proper orientation of the mitotic spindle. Malfunctioning of these processes can considerably delay mitosis, thereby compromising tissue growth and homeostasis, and leading to chromosomal instability. Loss of functional Mms19 drastically affects the growth and development of mitotic tissues in Drosophila larvae and we now demonstrate that Mms19 is an important factor that promotes spindle and astral microtubule (MT) growth, and MT stability and bundling. Mms19 function is needed for the coordination of mitotic events and for the rapid progres

APA, Harvard, Vancouver, ISO und andere Zitierweisen

32

Sandquist, Joshua C., Matthew E. Larson, Sarah Woolner, Zhiwei Ding, and William M. Bement. "An interaction between myosin-10 and the cell cycle regulator Wee1 links spindle dynamics to mitotic progression in epithelia." Journal of Cell Biology 217, no. 3 (2018): 849–59. http://dx.doi.org/10.1083/jcb.201708072.

Der volle Inhalt der Quelle

Annotation:

Anaphase in epithelia typically does not ensue until after spindles have achieved a characteristic position and orientation, but how or even if cells link spindle position to anaphase onset is unknown. Here, we show that myosin-10 (Myo10), a motor protein involved in epithelial spindle dynamics, binds to Wee1, a conserved regulator of cyclin-dependent kinase 1 (Cdk1). Wee1 inhibition accelerates progression through metaphase and disrupts normal spindle dynamics, whereas perturbing Myo10 function delays anaphase onset in a Wee1-dependent manner. Moreover, Myo10 perturbation increases Wee1-media

APA, Harvard, Vancouver, ISO und andere Zitierweisen

33

Keshri, Riya, Ashwathi Rajeevan та Sachin Kotak. "PP2A-B55γ counteracts Cdk1 and regulates proper spindle orientation through the cortical dynein adaptor NuMA". Journal of Cell Science 133, № 14 (2020): jcs243857. http://dx.doi.org/10.1242/jcs.243857.

Der volle Inhalt der Quelle

Annotation:

ABSTRACTProper orientation of the mitotic spindle is critical for accurate development and morphogenesis. In human cells, spindle orientation is regulated by the evolutionarily conserved protein NuMA, which interacts with dynein and enriches it at the cell cortex. Pulling forces generated by cortical dynein orient the mitotic spindle. Cdk1-mediated phosphorylation of NuMA at threonine 2055 (T2055) negatively regulates its cortical localization. Thus, only NuMA not phosphorylated at T2055 localizes at the cell cortex. However, the identity and the mechanism of action of the phosphatase complex

APA, Harvard, Vancouver, ISO und andere Zitierweisen

34

Zheng, Zhen, Huabin Zhu, Qingwen Wan, et al. "LGN regulates mitotic spindle orientation during epithelial morphogenesis." Journal of Cell Biology 189, no. 2 (2010): 275–88. http://dx.doi.org/10.1083/jcb.200910021.

Der volle Inhalt der Quelle

Annotation:

Coordinated cell polarization and mitotic spindle orientation are thought to be important for epithelial morphogenesis. Whether spindle orientation is indeed linked to epithelial morphogenesis and how it is controlled at the molecular level is still unknown. Here, we show that the NuMA- and Gα-binding protein LGN is required for directing spindle orientation during cystogenesis of MDCK cells. LGN localizes to the lateral cell cortex, and is excluded from the apical cell cortex of dividing cells. Depleting LGN, preventing its cortical localization, or disrupting its interaction with endogenous

APA, Harvard, Vancouver, ISO und andere Zitierweisen

35

Machicoane, Mickael, Cristina A. de Frutos, Jenny Fink, et al. "SLK-dependent activation of ERMs controls LGN–NuMA localization and spindle orientation." Journal of Cell Biology 205, no. 6 (2014): 791–99. http://dx.doi.org/10.1083/jcb.201401049.

Der volle Inhalt der Quelle

Annotation:

Mitotic spindle orientation relies on a complex dialog between the spindle microtubules and the cell cortex, in which F-actin has been recently implicated. Here, we report that the membrane–actin linkers ezrin/radixin/moesin (ERMs) are strongly and directly activated by the Ste20-like kinase at mitotic entry in mammalian cells. Using microfabricated adhesive substrates to control the axis of cell division, we found that the activation of ERMs plays a key role in guiding the orientation of the mitotic spindle. Accordingly, impairing ERM activation in apical progenitors of the mouse embryonic ne

APA, Harvard, Vancouver, ISO und andere Zitierweisen

36

Fraschini, Roberta, Denis Bilotta, Giovanna Lucchini, and Simonetta Piatti. "Functional Characterization of Dma1 and Dma2, the Budding Yeast Homologues of Schizosaccharomyces pombe Dma1 and Human Chfr." Molecular Biology of the Cell 15, no. 8 (2004): 3796–810. http://dx.doi.org/10.1091/mbc.e04-02-0094.

Der volle Inhalt der Quelle

Annotation:

Proper transmission of genetic information requires correct assembly and positioning of the mitotic spindle, responsible for driving each set of sister chromatids to the two daughter cells, followed by cytokinesis. In case of altered spindle orientation, the spindle position checkpoint inhibits Tem1-dependent activation of the mitotic exit network (MEN), thus delaying mitotic exit and cytokinesis until errors are corrected. We report a functional analysis of two previously uncharacterized budding yeast proteins, Dma1 and Dma2, 58% identical to each other and homologous to human Chfr and Schizo

APA, Harvard, Vancouver, ISO und andere Zitierweisen

37

McHugh, Toni, Agata A. Gluszek, and Julie P. I. Welburn. "Microtubule end tethering of a processive kinesin-8 motor Kif18b is required for spindle positioning." Journal of Cell Biology 217, no. 7 (2018): 2403–16. http://dx.doi.org/10.1083/jcb.201705209.

Der volle Inhalt der Quelle

Annotation:

Mitotic spindle positioning specifies the plane of cell division during anaphase. Spindle orientation and positioning are therefore critical to ensure symmetric division in mitosis and asymmetric division during development. The control of astral microtubule length plays an essential role in positioning the spindle. In this study, using gene knockout, we show that the kinesin-8 Kif18b controls microtubule length to center the mitotic spindle at metaphase. Using in vitro reconstitution, we reveal that Kif18b is a highly processive plus end–directed motor that uses a C-terminal nonmotor microtub

APA, Harvard, Vancouver, ISO und andere Zitierweisen

38

Zellag, Réda M., Yifan Zhao, Vincent Poupart, Ramya Singh, Jean-Claude Labbé, and Abigail R. Gerhold. "CentTracker: a trainable, machine-learning–based tool for large-scale analyses of Caenorhabditis elegans germline stem cell mitosis." Molecular Biology of the Cell 32, no. 9 (2021): 915–30. http://dx.doi.org/10.1091/mbc.e20-11-0716.

Der volle Inhalt der Quelle

Annotation:

We provide technical and analytical tools to facilitate large-scale intravital imaging studies of Caenorhabditis elegans germline stem cell (GSC) mitosis, which are expandable to other model systems. We assess multiple GSC mitotic features and find evidence for spatial clustering of divisions and a spindle orientation bias within the stem cell pool.

APA, Harvard, Vancouver, ISO und andere Zitierweisen

39

Kosetsu, Ken, Takashi Murata, Moé Yamada, et al. "Cytoplasmic MTOCs control spindle orientation for asymmetric cell division in plants." Proceedings of the National Academy of Sciences 114, no. 42 (2017): E8847—E8854. http://dx.doi.org/10.1073/pnas.1713925114.

Der volle Inhalt der Quelle

Annotation:

Proper orientation of the cell division axis is critical for asymmetric cell divisions that underpin cell differentiation. In animals, centrosomes are the dominant microtubule organizing centers (MTOC) and play a pivotal role in axis determination by orienting the mitotic spindle. In land plants that lack centrosomes, a critical role of a microtubular ring structure, the preprophase band (PPB), has been observed in this process; the PPB is required for orienting (before prophase) and guiding (in telophase) the mitotic apparatus. However, plants must possess additional mechanisms to control the

APA, Harvard, Vancouver, ISO und andere Zitierweisen

40

Hwang, Hyung-Seo, and Kiwon Song. "IBD2 Encodes a Novel Component of the Bub2p-Dependent Spindle Checkpoint in the Budding Yeast Saccharomyces cerevisiae." Genetics 161, no. 2 (2002): 595–609. http://dx.doi.org/10.1093/genetics/161.2.595.

Der volle Inhalt der Quelle

Annotation:

Abstract During mitosis, genomic integrity is maintained by the proper coordination of mitotic events through the spindle checkpoint. The bifurcated spindle checkpoint blocks cell cycle progression at metaphase by monitoring unattached kinetochores and inhibits mitotic exit in response to the incorrect orientation of the mitotic spindle. Bfa1p is a spindle checkpoint regulator of budding yeast in the Bub2p checkpoint pathway for proper mitotic exit. We have isolated a novel Bfa1p interacting protein named Ibd2p in the budding yeast Saccharomyces cerevisiae. We found that IBD2 (Inhibition of Bu

APA, Harvard, Vancouver, ISO und andere Zitierweisen

41

Molla, Annie. "Aurora kinases orchestrate mitosis; who are the players?" BioMolecular Concepts 1, no. 2 (2010): 147–55. http://dx.doi.org/10.1515/bmc.2010.014.

Der volle Inhalt der Quelle

Annotation:

AbstractThe Aurora are a conserved family of serine/threonine kinases with essential functions in cell division. In mitosis, Aurora kinases are required for chromosome segregation, condensation and orientation in the metaphase plate, spindle assembly, and the completion of cytokinesis. This review presents the Aurora kinases, their partners and how their interactions impact on the different mitotic functions.

APA, Harvard, Vancouver, ISO und andere Zitierweisen

42

Varma, Dileep, Pascale Monzo, Stephanie A. Stehman, and Richard B. Vallee. "Direct role of dynein motor in stable kinetochore-microtubule attachment, orientation, and alignment." Journal of Cell Biology 182, no. 6 (2008): 1045–54. http://dx.doi.org/10.1083/jcb.200710106.

Der volle Inhalt der Quelle

Annotation:

Cytoplasmic dynein has been implicated in diverse mitotic functions, several involving its association with kinetochores. Much of the supporting evidence comes from inhibition of dynein regulatory factors. To obtain direct insight into kinetochore dynein function, we expressed a series of dynein tail fragments, which we find displace motor-containing dynein heavy chain (HC) from kinetochores without affecting other subunits, regulatory factors, or microtubule binding proteins. Cells with bipolar mitotic spindles progress to late prometaphase-metaphase at normal rates. However, the dynein tail,

APA, Harvard, Vancouver, ISO und andere Zitierweisen

43

Schoborg, Todd, Allison L. Zajac, Carey J. Fagerstrom, Rodrigo X. Guillen, and Nasser M. Rusan. "An Asp–CaM complex is required for centrosome–pole cohesion and centrosome inheritance in neural stem cells." Journal of Cell Biology 211, no. 5 (2015): 987–98. http://dx.doi.org/10.1083/jcb.201509054.

Der volle Inhalt der Quelle

Annotation:

The interaction between centrosomes and mitotic spindle poles is important for efficient spindle formation, orientation, and cell polarity. However, our understanding of the dynamics of this relationship and implications for tissue homeostasis remains poorly understood. Here we report that Drosophila melanogaster calmodulin (CaM) regulates the ability of the microcephaly-associated protein, abnormal spindle (Asp), to cross-link spindle microtubules. Both proteins colocalize on spindles and move toward spindle poles, suggesting that they form a complex. Our binding and structure–function analys

APA, Harvard, Vancouver, ISO und andere Zitierweisen

44

Heald, Rebecca, Régis Tournebize, Anja Habermann, Eric Karsenti, and Anthony Hyman. "Spindle Assembly in Xenopus Egg Extracts: Respective Roles of Centrosomes and Microtubule Self-Organization." Journal of Cell Biology 138, no. 3 (1997): 615–28. http://dx.doi.org/10.1083/jcb.138.3.615.

Der volle Inhalt der Quelle

Annotation:

In Xenopus egg extracts, spindles assembled around sperm nuclei contain a centrosome at each pole, while those assembled around chromatin beads do not. Poles can also form in the absence of chromatin, after addition of a microtubule stabilizing agent to extracts. Using this system, we have asked (a) how are spindle poles formed, and (b) how does the nucleation and organization of microtubules by centrosomes influence spindle assembly? We have found that poles are morphologically similar regardless of their origin. In all cases, microtubule organization into poles requires minus end–directed tr

APA, Harvard, Vancouver, ISO und andere Zitierweisen

45

Yang, Zhenye, Jing Guo, Qi Chen, Chong Ding, Juan Du, and Xueliang Zhu. "Silencing Mitosin Induces Misaligned Chromosomes, Premature Chromosome Decondensation before Anaphase Onset, and Mitotic Cell Death." Molecular and Cellular Biology 25, no. 10 (2005): 4062–74. http://dx.doi.org/10.1128/mcb.25.10.4062-4074.2005.

Der volle Inhalt der Quelle

Annotation:

ABSTRACT Mitosin (also named CENP-F) is a large human nuclear protein transiently associated with the outer kinetochore plate in M phase. Using RNA interference and fluorescence microscopy, we showed that mitosin depletion attenuated chromosome congression and led to metaphase arrest with misaligned polar chromosomes whose kinetochores showed few cold-stable microtubules. Kinetochores of fully aligned chromosomes often failed to show orientation in the direction of the spindle long axis. Moreover, tension across their sister kinetochores was decreased by 53% on average. These phenotypes collec

APA, Harvard, Vancouver, ISO und andere Zitierweisen

46

Meadows, John C. "Interplay between mitotic kinesins and the Aurora kinase–PP1 (protein phosphatase 1) axis." Biochemical Society Transactions 41, no. 6 (2013): 1761–65. http://dx.doi.org/10.1042/bst20130191.

Der volle Inhalt der Quelle

Annotation:

Correct transmission of genetic information from mother to daughter cells is necessary for development and survival. Accurate segregation is achieved by bipolar attachment of sister kinetochores in each chromatid pair to spindle microtubules emanating from opposite spindle poles, a process known as chromosome bi-orientation. Achieving this requires dynamic interplay between kinetochore proteins, kinesin motor proteins and cell cycle regulators. Chromosome bi-orientation is monitored by a surveillance mechanism known as the SAC (spindle assembly checkpoint). The Aurora B kinase, which is bound

APA, Harvard, Vancouver, ISO und andere Zitierweisen

47

Levesque, Aime A., and Duane A. Compton. "The chromokinesin Kid is necessary for chromosome arm orientation and oscillation, but not congression, on mitotic spindles." Journal of Cell Biology 154, no. 6 (2001): 1135–46. http://dx.doi.org/10.1083/jcb.200106093.

Der volle Inhalt der Quelle

Annotation:

Chromokinesins have been postulated to provide the polar ejection force needed for chromosome congression during mitosis. We have evaluated that possibility by monitoring chromosome movement in vertebrate-cultured cells using time-lapse differential interference contrast microscopy after microinjection with antibodies specific for the chromokinesin Kid. 17.5% of cells injected with Kid-specific antibodies have one or more chromosomes that remain closely opposed to a spindle pole and fail to enter anaphase. In contrast, 82.5% of injected cells align chromosomes in metaphase, progress to anaphas

APA, Harvard, Vancouver, ISO und andere Zitierweisen

48

Efimov, Vladimir P., and N. Ronald Morris. "A Screen for Dynein Synthetic Lethals in Aspergillus nidulans Identifies Spindle Assembly Checkpoint Genes and Other Genes Involved in Mitosis." Genetics 149, no. 1 (1998): 101–16. http://dx.doi.org/10.1093/genetics/149.1.101.

Der volle Inhalt der Quelle

Annotation:

Abstract Cytoplasmic dynein is a ubiquitously expressed microtubule motor involved in vesicle transport, mitosis, nuclear migration, and spindle orientation. In the filamentous fungus Aspergillus nidulans, inactivation of cytoplasmic dynein, although not lethal, severely impairs nuclear migration. The role of dynein in mitosis and vesicle transport in this organism is unclear. To investigate the complete range of dynein function in A. nidulans, we searched for synthetic lethal mutations that significantly reduced growth in the absence of dynein but had little effect on their own. We isolated 1

APA, Harvard, Vancouver, ISO und andere Zitierweisen

49

Kuo, Wei-Ting, Li Zuo, and Jerrold Turner. "THE TIGHT JUNCTION PROTEIN ZO-1 REGULATES MITOTIC SPINDLE ORIENTATION TO ENABLE EFFICIENT MUCOSAL REPAIR." Inflammatory Bowel Diseases 27, Supplement_1 (2021): S28. http://dx.doi.org/10.1093/ibd/izaa347.064.

Der volle Inhalt der Quelle

Annotation:

Abstract The intestinal damage can be caused by physical, infectious, and immune-mediated injury. Rapid repair, which is essential for a return to mucosal homeostasis, depends on rapid epithelial migration, i.e., restitution, as well as epithelial proliferation and differentiation. Barrier restoration is a critical component of this repair process, but the contributions of structural proteins that form the barrier to mucosal repair have not been defined. Aim: To determine the contributions of the intestinal epithelial tight junction protein zonula occludens-1 (ZO-1) to mucosal repair. Results:

APA, Harvard, Vancouver, ISO und andere Zitierweisen

50

Nestor-Bergmann, Alexander, Georgina Goddard, and Sarah Woolner. "Force and the spindle: Mechanical cues in mitotic spindle orientation." Seminars in Cell & Developmental Biology 34 (October 2014): 133–39. http://dx.doi.org/10.1016/j.semcdb.2014.07.008.

Der volle Inhalt der Quelle

APA, Harvard, Vancouver, ISO und andere Zitierweisen

Wir bieten Rabatte auf alle Premium-Pläne für Autoren, deren Werke in thematische Literatursammlungen aufgenommen wurden. Kontaktieren Sie uns, um einen einzigartigen Promo-Code zu erhalten!