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1

Austin, Eric B. "Human monoclonal antibodies." Thesis, University of Nottingham, 1990. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.276187.

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2

Plumpton, Christopher. "Monoclonal antibodies against phytochrome." Thesis, University of Cambridge, 1990. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.358677.

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3

Benjamin, Richard John. "Tolerance induction with monoclonal antibodies." Thesis, University of Cambridge, 1987. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.253988.

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4

Qin, Shi-Xin. "Transplantation tolerance with monoclonal antibodies." Thesis, University of Cambridge, 1989. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.305697.

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5

Heron, Andrew David. "The stability of monoclonal antibodies." Thesis, University of Glasgow, 1999. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.252169.

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6

Isaacs, John Dudley. "Improving serotherapy with monoclonal antibodies." Thesis, University of Cambridge, 1991. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.386115.

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7

Paudel, Subhash. "Shear thinning in monoclonal antibodies." Thesis, Kansas State University, 2016. http://hdl.handle.net/2097/32833.

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Master of Science<br>Department of Physics<br>Jeremy D. Schmit<br>Antibodies are large Y-shaped proteins which are used by immune system to identify and neutralize pathogens. Monoclonal antibody therapy is used to treat different patient conditions. There are problems associated with the manufacturability and deliverability of mAb solutions due to the viscous nature of the protein. The viscosity of antibody solutions increases with the increase in concentration and decreases with applied shear. We want to know why these behaviours are seen and to address this problem we have developed a theory
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8

Ueda, Yasuji. "MONOCLONAL ANTIBODIES TO CHICK CRYSTALLINS." 京都大学 (Kyoto University), 1989. http://hdl.handle.net/2433/86412.

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9

Pathan, N. "Catalytic monoclonal antibodies: a review." Thesis(M.Phil.), CSIR-National Chemical Laboratory, Pune, 1990. http://dspace.ncl.res.in:8080/xmlui/handle/20.500.12252/2017.

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10

Alexandrovich, Susan K. "Characterization of monoclonal antibodies against digoxin /." Online version of thesis, 1987. http://hdl.handle.net/1850/10681.

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11

Mirza, Myriam. "Characterization of new CFTR monoclonal antibodies." Thesis, McGill University, 2009. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=66882.

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The available antibodies against CFTR are not sensitive enough to detect CFTR at endogenous or near endogenous levels making detection at native levels difficult. We raised two monoclonal antibodies, 22E8 and 23C5, against the R domain of human CFTR with the goal of identifying an antibody sensitive enough to detect CFTR in native airway cells. These antibodies were characterized for their ability to detect over-expressed as well as endogenous levels of CFTR in immunoblotting, immunoprecipitation and immunofluorescence. Their ability to detect CFTR was also compared with comm
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12

Noble, Philip W. "Characterisation of anti-glycan monoclonal antibodies." Thesis, University of Nottingham, 2011. http://eprints.nottingham.ac.uk/12071/.

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The aims of this thesis are to establish the therapeutic value of two anti-glycan mAbs produced in-house, to develop an immunisation protocol with the aim of improving the immunogenicity tumour-associated glycolipids with the intention of producing therapeutically valuable mAbs and to determine the implication of a mAb with the ability to induce apoptosis in colorectal cancer. The anti-glycan mAbs 692/29 and 505/4 have previously been produced in-house and this study aimed to determine their fine specificity using a glycan array. 692/29 displayed binding predominantly to Lewis b as well as Lew
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13

Thanh, Le Thiet. "Exon-specific monoclonal antibodies against dystrophin." Thesis, University of Salford, 1995. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.261661.

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14

Watson, Nigel. "Monoclonal antibodies to human immunoglobulin allotypes." Thesis, University of Strathclyde, 1988. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.304897.

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15

Ortlepp, Susan. "Leucocyte integrin activation by monoclonal antibodies." Thesis, Open University, 1997. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.359976.

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16

Holdsworth, Mary Louise. "Characterisation of phytochrome using monoclonal antibodies." Thesis, University of Leicester, 1987. http://hdl.handle.net/2381/35466.

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Characterisation of phytochrome using monoclonal antibodies Mary L. Holdsworth Native oat phytochrome has been purified to homogeneity and used to produce a panel of monoclonal antibodies (mAbs). Selection of mAbs followed early screening against native phytochrome by ELISA, and SDS-denatured phytochrome by "mini" western blotting. Six mAbs which recognised SDS-denatured phytochrome were mapped using proteolytically derived fragments of phytochrome and subsequent immunoblotting. LAS 31 and 33 map to the 6 kDa NH2-terminus and LAS 35 and 41 map to the adjacent 4 kDa sub-NH2- terminal domain. LA
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17

Giorno, Caterina [Verfasser]. "Glycoengineering of Monoclonal Antibodies / Caterina Giorno." Konstanz : Bibliothek der Universität Konstanz, 2010. http://d-nb.info/1020366117/34.

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18

Yeung, Douglas Edward. "Characterization of six monoclonal antibodies against the Minute Virus of Mice NS-1 protein, and the use of one in the immunoaffinity purification of NS-1 expressed in insect cells." Thesis, University of British Columbia, 1990. http://hdl.handle.net/2429/29405.

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Six mouse monoclonal antibodies have been isolated which react against a bacterial fusion protein containing amino acids 364 to 623 of the NS-1 protein of the prototype strain of the Minute Virus of Mice (MVMp). All six were found to be of the IgG class of antibodies; five being IgG₁ and the sixth being IgG₂[formula omitted]. By immunoblot analyses, these antibodies all recognize an 83 kDa protein found only in MVM-infected mouse fibroblast cells, leading to the assumption that they are all NS-1 specific. Further evidence for this assumption is obtained from indirect immunofluorescence studies
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19

Ohlin, Mats. "Human monoclonal antibody technology a tool to investigate human antibody repertoires /." Lund : Dept. of Immunotechnology, Lund University, 1992. http://catalog.hathitrust.org/api/volumes/oclc/39693827.html.

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20

Evans, Rachael Yvonne. "The production of anti-idiotopic antibodies to monoclonal anti-RhD antibodies." Thesis, Lancaster University, 2001. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.274194.

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21

Chen, Desheng, and chen desheng@deakin edu au. "Development of monoclonal antibodies against Vibrio pathogens." Deakin University. Department of Biological Science, 1991. http://tux.lib.deakin.edu.au./adt-VDU/public/adt-VDU20080626.140825.

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Monoclonal antibodies were developed against pathogenic vibrios for use in rapid identification in disease situations of humans, fish and shellfish. Of the 12 fusions performed using V. alginolyticus, V. anguillarum, V. carchariae, V. cholerae, V. damsela, V. furnissii, V. harveyi, V. ordalii, V. parahaemolyticus and V. vulnificus, a total of 102 hybridomas were obtained. Based on cross-reactivity of a wide range of V
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22

Bell, Ian Martin. "Monoclonal antibodies as catalysts for cationic cyclisations." Thesis, University of Cambridge, 1991. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.387041.

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23

Storey, E. "Monoclonal antibodies to merozoites of Plasmodium falciparum." Thesis, University of Oxford, 1986. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.371577.

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24

Banbury, David N. "New monoclonal antibodies to visualise vesicular compartments." Thesis, University of Oxford, 1994. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.259782.

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25

Klutz, Stephan [Verfasser]. "Continuous processing of monoclonal antibodies / Stephan Klutz." München : Verlag Dr. Hut, 2016. http://d-nb.info/1115549731/34.

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26

Honey, C. R. "Immunosuppression with monoclonal antibodies in neural transplantation." Thesis, University of Oxford, 1990. http://ora.ox.ac.uk/objects/uuid:ea39dc7a-4ada-4c21-8cef-4649cb322646.

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27

Huang, Ling. "Investigation of soya globulins using monoclonal antibodies." Thesis, University of East Anglia, 1998. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.302022.

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28

Anderson, J. "Investigations on bluetongue virus using monoclonal antibodies." Thesis, Open University, 1986. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.374892.

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The main aim of the thesis was the production and characterisation of monoclonal antibodies (Mabs) against bluetongue virus (BTV) and the application of such antibodies to the improvement of BTV diagnosis. The suitability of the indirect ELISA for the detection of antibodies to BTV was examined along with the published protocols for the purification of BTV ELISA antigen. When sera were examined from animals which had experienced either BTV vaccine or any other tissue-culture derived vaccine, host-cell protein contaminants in the BTV ELISA antigen reacted with anti-BHK cell antibodies to give f
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29

Jones, Christine Ann. "Monoclonal antibodies in the study of neuropeptides." Thesis, Imperial College London, 1991. http://hdl.handle.net/10044/1/46848.

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30

Yeates, Peter Gregory. "Tumour imaging using hCG-specific monoclonal antibodies." Thesis, The University of Sydney, 1989. https://hdl.handle.net/2123/26188.

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The aim of this thesis was to radiolabel two different anti-hCG antibodies with 111-indium, and to determine their specific tumour uptake and tissue biodistribution into choriocarcinoma xenografts in nude mice against 111-indium and 67-gallium citrate over 72 hours. Comparative radioimmunoscintigraphy was performed to determine the optimal imaging post injection for visualisation of tumour sites. Data manipulation using dual isotope computerised subtraction in mouse phantom models was evaluated as means of improving tumour delineation in low tumour contrast studies.
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31

Chen, Jianqing. "Radiolabeling and biotinylation of internalizing monoclonal antibody chimeric BR96 potential use for extracorporeal immunoadsorption with enhanced tumor radioactivity retention of iodine, indium and rhenium /." Lund : Lund University, the Jubileum Institute, Dept. of Radiation Physics, 1997. http://catalog.hathitrust.org/api/volumes/oclc/39725797.html.

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32

Xu, Wenbin. "Studies of antigenic relationships among spotted fever group rickettsiae by monoclonal antibodies." Aix-Marseille 2, 1997. http://www.theses.fr/1997AIX20665.

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33

Hills, Anna E. "Control of monoclonal antibody N-glycosylation." Thesis, University of Kent, 2001. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.344101.

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34

Sun, Ping. "Studies of sperm antigens using specific monoclonal antibodies." Thesis, University of British Columbia, 1987. http://hdl.handle.net/2429/28405.

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This thesis work is focused on studies of sperm antigen: its purification and antifertility effect, its "internal image" by use of anti-idiotypic antibodies, and its capacity to induce antibodies of IgA class. Mouse sperm acrosomal antigens that react with a monoclonal antibody generated against mouse spermatozoa, MS 207, were purified by immunoaffinity chromatography from soluble fraction of mouse testis homogenates. Analyzed by HPLC and SDS gel electrophoresis in the presence of reducing agent, the purified sperm antigens were shown to have a molecular weight of 600 to 700 KDa. It was shown
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35

Tsim, Karl Wah-Keung. "Chick acetylcholinesterase : purification, molecular properties and monoclonal antibodies." Thesis, University of Cambridge, 1987. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.236054.

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Acetylcholinesterase (AChE, E.C. 3.1.1.7) from 1-day chick brain was enriched over 2,000-fold by N-methylacridinium affinity column. Using this preparation as immunogen, two monoclonal antibodies (mAbs), 1E2 and 3D10, were isolated. Both mAbs react with all molecular forms of AChE from chick but not with butyrylcholinesterases (BuChE, E.C. 3.1.1.8). The mAb, 1E2, was used to immunopurify chick brain globular and muscle asymmetric AChE to homogeneity. The purified brain AChE showed a specific activity of 2,200 U/mg of protein, and it appeared to be a hydrophobic tetramer with a subunit mass of
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36

Gilliland, Lisa Kim. "The development of bispecific monoclonal antibodies for therapy." Thesis, University of Cambridge, 1987. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.278214.

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37

Watkins, I. D. "Monoclonal antibodies to Legionella pneumophila and related organisms." Thesis, University of Oxford, 1985. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.354879.

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38

Tellides, George. "Immunosupression monoclonal antibodies to rat lymphocyte activation antigens." Thesis, University of Oxford, 1988. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.236271.

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39

Chia, T. W. H. "The use of monoclonal antibodies for glycoside hydrolysis." Thesis, University of Cambridge, 1993. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.597597.

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Since 1986, a wide range of reactions have been catalysed by monoclonal antibodies, many with good rate enhancements, high regio- and stereo-selectivities and high substrate specificities. As antibodies can be elicited against practically any molecule of interest, this new class of antibody molecules, commonly known as abzymes, offers a unique way of generating tailor-made, enzyme-like catalysts. We propose to investigate the possibility of using monoclonal antibodies to catalyse the hydrolysis of glycosides. Glycosides are important components of many biological processes especially in glycop
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40

Mason, Caroline Margaret. "Characterisation of intestinal M cells using monoclonal antibodies." Thesis, University of Newcastle Upon Tyne, 1994. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.260952.

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41

Perera, W. Shermal. "Binding and molecular characterisation of monoclonal RhD antibodies." Thesis, University of Aberdeen, 1999. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.342706.

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Monoclonal RhD antibodies (anti-RhD) may potentially be used in preventing Rh alloimmunisation of women delivering RhD-positive babies, and thereby preventing haemolytic disease of the newborn (HDN). Binding and molecular analyses were performed on a range of monoclonal anti-RhDs to understand the interaction between the antibody and antigen. A flow cytometry (FCM) assay system was developed to analyse the binding of the anti-RhD to human group O R<sub>1</sub>R<sub>2</sub> red blood cells (RBC). A panel of 30 anti-RhD preparations were studied using this assay and Vmax (maximum binding) and KD
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42

Duguid, I. G. M. "Prevention of corneal graft rejection with monoclonal antibodies." Thesis, University of Aberdeen, 1992. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.387460.

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This thesis aims to place corneal allograft rejection in the context of general transplantation immunology, examine the role of lymphocyte subsets in the rejection process and consider the potential application of monoclonal antibody therapy in clinical corneal graft rejection. The literature relating to the current clinical practice of corneal grafting, with particular reference to corneal allograft rejection, is reviewed in chapter 1 to present the extent of the problem. Chapter 2 then reviews the mechanisms of allograft rejection from the literature of transplantation immunology, much of wh
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43

De, Silva M. G. "Human monoclonal antibodies in the study of diabetes." Thesis, University of Surrey, 1988. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.233146.

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44

Bell, Graham Thomas. "Studies on the production of human monoclonal antibodies." Thesis, University of Edinburgh, 1988. http://hdl.handle.net/1842/19241.

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45

Buffetto, Fanny. "Generation of new monoclonal antibodies to rhamnogalacturonan fragments." Nantes, 2014. https://archive.bu.univ-nantes.fr/pollux/show/show?id=85b86558-be8b-4c38-ae82-702e14160fa0.

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Les pectines sont largement présentes dans la lamelle moyenne et la paroi primaire des dicotylédones. Elles sont composées de différents domaines structuraux liés de façon covalente. Les rhamnogalacturonanes II et les rhamnogalacturonanes I sont les domaines pectiques les plus complexes. Le rhamnogalacturonane II est formé d'une chaîne principale d'homogalacturonane sur laquelle sont branchées 5 chaînes latérales contenant de nombreux oses rares. Le rhamnoglaladuronane·I possède un squelette·hétérogène de rhamnoses et d'acides galacturoniques. Ce domaine contient des ramifications constituées
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46

Eastwood, David Geoffrey Douglas. "Immunotoxicology of the therapeutic monoclonal antibody TGN1412." Thesis, St George's, University of London, 2015. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.676898.

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Having passed all pre-clinical safety testing, the superagonistic anti -CD28 therapeutic monoclonal antibody (mAb ) TGN 1412, intended for the treatment of rheumatoid arthritis and B-cell chronic lymphocytic leukaemia, was approved by German and UK regulatory authorities for first-in-man Phase One clinical trial. Shortly after infusion, all six healthy trial volunteers suffered unexpected and profound systemic pro-inflammatory cytokine release, later termed a 'cytokine storm,' causing multi-organ failure. This unexpected and near fatal cytokine release syndrome (CRS) publically highlighted the
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47

Kalofonos, Haralabos. "Radiolabelled monoclonal antibodies for tumour immunoscintigraphy and biodistribution studies." Thesis, Imperial College London, 1990. http://hdl.handle.net/10044/1/46376.

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48

Joyce, Christopher Francis. "The production and functional assessment of IGA monoclonal antibodies." Thesis, Imperial College London, 1990. http://hdl.handle.net/10044/1/46374.

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49

Brush, Bradley Alan. "Characterization of monoclonal antibodies to rubella virus structural proteins." Thesis, University of British Columbia, 1989. http://hdl.handle.net/2429/27400.

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Monoclonal antibodies (MAbs), due to their intrinsic specificity to single determinants, are capable of defining the antigenic and structural properties of viruses. A panel of twenty-five monoclonal antibodies, the result of fusions between splenocytes from Balb/c mice immunized with RA 27/3 vaccine strain rubella virus and myeloma cell line NS1X63Ag8.653, were characterized by radioimmunoprecipitation of rubella virus structural proteins, IgG sub-classification, haemagglutination inhibition (HI) and virus neutralization (Nt) of rubella virus (RV). The antigen specificities of thirteen
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50

Hunter, Nicole Marie. "A system for the production of human monoclonal antibodies." Thesis, National Library of Canada = Bibliothèque nationale du Canada, 1999. http://www.collectionscanada.ca/obj/s4/f2/dsk1/tape8/PQDD_0006/MQ46024.pdf.

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