Auswahl der wissenschaftlichen Literatur zum Thema „Motility and morphology“
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Zeitschriftenartikel zum Thema "Motility and morphology":
NAKAMURA, Shuichi. „Morphology and motility of the spirochetes“. Nippon Saikingaku Zasshi 69, Nr. 3 (2014): 527–38. http://dx.doi.org/10.3412/jsb.69.527.
Krockova, J., S. Roychoudhury, T. Slanina, G. Formicki, L. J. Binkowski, L. Ondruska, N. Lukac, R. Kovacova, R. Stawarz und P. Massanyi. „Lead induced alterations in rabbit spermatozoa motility and morphology in vitro“. Czech Journal of Animal Science 61, No. 9 (27.09.2016): 391–406. http://dx.doi.org/10.17221/58/2015-cjas.
SCHATTE, EDWARD C., STEVEN J. HIRSHBERG, MARK L. FALLICK, LARRY I. LIPSHULTZ und EDWARD D. KIM. „VARICOCELECTOMY IMPROVES SPERM STRICT MORPHOLOGY AND MOTILITY“. Journal of Urology 160, Nr. 4 (Oktober 1998): 1338–40. http://dx.doi.org/10.1016/s0022-5347(01)62531-x.
Jeyaraju, Danny V., Giulia Cisbani und Luca Pellegrini. „Calcium regulation of mitochondria motility and morphology“. Biochimica et Biophysica Acta (BBA) - Bioenergetics 1787, Nr. 11 (November 2009): 1363–73. http://dx.doi.org/10.1016/j.bbabio.2008.12.005.
Darsini, Ninik, Berliana Hamidah, Seso Sulijaya Suyono, Faisal Yusuf Ashari, R. Haryanto Aswin und Rina Yudiwati. „Human Sperm Motility, Viability, and Morphology Decreased after Cryopreservation“. Folia Medica Indonesiana 55, Nr. 3 (03.10.2019): 198. http://dx.doi.org/10.20473/fmi.v55i3.15501.
Kastelic, JP, RE Wilde, G. Rizzoto und JC Thundathil. „Hyperthermia and not hypoxia may reduce sperm motility and morphology following testicular hyperthermia“. Veterinární Medicína 62, No. 8 (24.08.2017): 437–42. http://dx.doi.org/10.17221/124/2016-vetmed.
Nikolovski, Martin, Monika Dovenska, Ksenija Ilievska, Nikola Adamov, Branko Atanasov, Miroslav Radeski, Daniela Kirovski, Vladimir Petkov und Toni Dovenski. „Homologous Seminal Plasma and Glutathione Promote Pre-Capacitation Motility and Structural Stability of Cryopreserved Ram Spermatozoa“. Macedonian Veterinary Review 42, Nr. 2 (01.10.2019): 169–79. http://dx.doi.org/10.2478/macvetrev-2019-0022.
Lampiao, Fanuel, und Joseph Chisaka. „Incidence and impact of hyperviscosity on sperm parameters of Malawian men seeking assisted reproduction“. African Health Sciences 20, Nr. 1 (20.04.2020): 1–3. http://dx.doi.org/10.4314/ahs.v20i1.3.
Fatrin, Tiara, Salni Salni, Sri Nita, Rachmat Hidayat, Triwani Triwani, Joko Marwoto, Ziske Maritska et al. „The Efficacy of Temu Putih Fraction (Curcuma Zedoaria (Berg) Roscoe) Related Quality and Quantity of Spermatozoa in Male Wistar Rats“. Bioscientia Medicina : Journal of Biomedicine and Translational Research 1, Nr. 1 (31.10.2017): 14–21. http://dx.doi.org/10.32539/bsm.v1i1.12.
Mladenović, I., S. Mićić, N. Papić, O. Genbaćev und B. Marinkovlć. „Sperm Morphology and Motility in Different Age Populations“. Archives of Andrology 32, Nr. 3 (Januar 1994): 197–205. http://dx.doi.org/10.3109/01485019408987786.
Dissertationen zum Thema "Motility and morphology":
Marth, Wieland. „Hydrodynamic Diffuse Interface Models for Cell Morphology and Motility“. Doctoral thesis, Saechsische Landesbibliothek- Staats- und Universitaetsbibliothek Dresden, 2016. http://nbn-resolving.de/urn:nbn:de:bsz:14-qucosa-204651.
Diese Dissertation beschäftigt sich mit mathematischen Modellen zur Beschreibung von Gleichgewichts- und dynamischen Zuständen von verallgemeinerten biologischen Zellen. Die Zellen werden dabei als thermodynamisches System aufgefasst, bei dem Strömungseffekte innerhalb und außerhalb der Zelle zusammen mit einem Helfrich-Modell für Zellmembranen kombiniert werden. Schließlich werden durch einen Energie-Variations-Ansatz die Evolutionsgleichungen für die Zelle hergeleitet. Es ergeben sie dabei Mehrphasen-Systeme, die Strömungseffekte mit einem freien Randwertproblem, das zusätzlich physikalischen Einflüssen wie Biegung und Oberflächenspannung unterliegt, vereinen. Um solche Probleme effizient zu lösen, wird in dieser Arbeit die Diffuse-Interface-Methode verwendet. Ein Vorteil dieser Methode ist, dass es sehr einfach möglich ist, Modelle, die verschiedenste Prozesse beschreiben, miteinander zu vereinen. Dies erlaubt es, komplexe biologische Phänomene, wie zum Beispiel Zellmotilität oder auch die kollektive Bewegung von Zellen, zu beschreiben. In den Modellen für Zellmotilität wird ein biologisches Netzwerk-Modell für GTPasen oder auch ein Active-Polar-Gel-Modell, das die Aktinfilamente im Inneren der Zellen als Flüssigkristall auffasst, mit dem Multi-Phasen-Modell kombiniert. Beide Modelle erlauben es, komplexe Vorgänge bei der selbst hervorgerufenen Bewegung von Zellen, wie das Vorantreiben der Zellmembran durch Aktinpolymerisierung oder auch die Kontraktionsbewegung des Zellkörpers durch kontraktile Spannungen innerhalb des Zytoskelets der Zelle, zu verstehen. Weiterhin ist die kollektive Bewegung von vielen Zellen von großem Interesse, da sich hier viele nichtlineare Phänomene zeigen. Um das Diffuse-Interface-Modell für eine Zelle auf die Beschreibung mehrerer Zellen zu übertragen, werden mehrere Phasenfelder eingeführt, die die Zellen jeweils kennzeichnen. Schließlich werden die Zellen durch ein lokales Abstoßungspotential gekoppelt. Das Modell wird angewendet, um White blood cell margination, das die Annäherung von Leukozyten an die Blutgefäßwand bezeichnet, zu verstehen. Dieser Prozess wird dabei bestimmt durch den komplexen Zusammenhang zwischen Kollisionen, den jeweiligen mechanischen Eigenschaften der Zellen, sowie deren Auftriebskraft innerhalb der Adern. Die Simulationen zeigen, dass diese Annäherung sich in bestimmten Gebieten des kardiovaskulären Systems stark vermindert, in denen die Blutströmung das Stokes-Regime verlässt. Schließlich wird das Active-Polar-Gel-Modell mit dem Modell für die kollektive Bewegung vom Zellen kombiniert. Dies macht es möglich, die kollektive Bewegung der Zellen und den Einfluss von Hydrodynamik auf diese Bewegung zu untersuchen. Es zeigt sich dabei, dass der Zustand der kollektiven gerichteten Bewegung sich spontan aus der Neuausrichtung der jeweiligen Zellen durch inelastische Kollisionen ergibt. Obwohl die Hydrodynamik einen großen Einfluss auf solche Systeme hat, deuten die Simulationen nicht daraufhin, dass Hydrodynamik die kollektive Bewegung vollständig unterdrückt. Weiterhin wird in dieser Arbeit gezeigt, wie die stark gekoppelten Systeme numerisch gelöst werden können mit Hilfe der Finiten-Elemente-Methode und wie die Effizienz der Methode gesteigert werden kann durch die Anwendung von Operator-Splitting-Techniken und Problemparallelisierung mittels OPENMP
Milner, David Stephen. „Regulatory genetics of cell morphology, motility and polarity in Bdellovibrio bacteriovorus“. Thesis, University of Nottingham, 2014. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.716668.
Wheeler, Ann. „The role of Rho GTPases in regulation of macrophage motility and morphology“. Thesis, University College London (University of London), 2005. http://discovery.ucl.ac.uk/1446723/.
Zhang, Yang. „A dynamical systems modelling framework for breast cancer cell motility and morphology analysis“. Thesis, University of Sheffield, 2017. http://etheses.whiterose.ac.uk/16014/.
DiGrassie, Wynne Aubin. „Evaluation of Stallion Frozen-Thawed Semen Using Conventional and Flow Cytometric Assays“. Thesis, Virginia Tech, 2000. http://hdl.handle.net/10919/33896.
In experiment one, six ejaculates were collected from each of three stallions. The semen from each ejaculate was centrifuged and frozen in 0.5 ml polyvinyl chloride straws. Two straws from each ejaculate were thawed and evaluated. Semen was evaluated for post-thaw motility, morphology, mitochondrial activity using Rhodamine 123 (R123), plasma membrane integrity using propidium iodide (PI) and ethidium monoazide (EMA), and chromatin structure using the sperm chromatin structure assay (SCSA). Data was recorded as percentages for all but the SCSA for both experiment one and two. The extent of chromatin denaturation was calculated using the SCSA and the alpha-t population [at = red/(red +green) fluorescence]. From the alpha-t population, statistics were calculated such mean (Xat), standard deviation (SDat), percentage of cells outside (COMPat) the main alpha-t population and the mean green fluorescence (mean green) of the population.
Results from experiment one demonstrated that all flow cytometric tests except EMA were able to distinguish between live and freeze-killed samples (p < 0.0001). Also the stallion accounted for most of the variation in samples when compared to ejaculate and straw within an ejaculate. Therefore two straws could be chosen at random from a stallion and evaluated in experiment two.
In experiment two, twenty-nine stallions were evaluated using the same tests as experiment one excluding EMA. Fertility data was obtained from the 1998 or 1999 breeding season. Multiple linear regression was used to determine the best-fit model to predict overall pregnancy rate. SCSA and R123-PI assays accounted for the largest amount of variation in fertility (R2 = 0.65, p < 0.0004). Within SCSA and the R123/PI assays Xat and PI staining had the highest contribution to this variation in fertility (R2 = 0.11, R2 = 0.47) respectively. The best-fit model for predicting fertility included the assay combination listed above and the interactions between SDat and mean green staining as well as R123 and mean green staining. Post-thaw motility and morphology did not account for significant variation in fertility (p = 0.22, p = 0.46) respectively.
Based on this project post-thaw motility and morphology are poor predictors of fertility in frozen-thawed stallion semen. However, through the addition of SCSA and R123-PI to the routine evaluation of frozen-thawed stallion semen time and money may be saved in advance by identifying those stallions with poor post-thaw fertility.
Master of Science
Rapuling, Llewelen. „Proteomic analysis of human sperm proteins in relation to sperm motility, morphology and energy metabolism“. Thesis, Stellenbosch : University of Stellenbosch, 2010. http://hdl.handle.net/10019.1/5205.
Thesis (MScMedSc (Biomedical Sciences. Medical Physiology))--University of Stellenbosch, 2010.
ENGLISH ABSTRACT: Male infertility is often associated with impaired sperm motility and morphology (asthenoteratozoospermia) for which there is no specific therapeutic treatment. It has come to light that the modification and expression of human sperm proteins play a crucial role in sperm function. In the present study, we present proteomic data of human spermatozoa in the context of sperm dysfunction. Novel techniques have been used to successfully isolate and identify differences in protein expression on a cellular level associated with asthenoteratozoospermia. In the first part of the study, differences in protein expression within the total sperm proteome were investigated between immature and mature sperm populations. Semen was collected from healthy donors (n=23) and separated into mature and immature sperm populations by 3-layer Percoll gradient centrifugation. Cells were washed and motility and morphology were measured by computer assisted sperm analysis (CASA). For the proteomic investigation cells were lysed and proteins separated by means of two-dimensional gel electrophoresis (2D electrophoresis). PD-Quest was used to identify the differentially expressed proteins. The protein spots of interest were excised and subjected to in-gel digestion. Peptides were separated by High Pressure Liquid Chromatography (HPLC) analysis and amino acid sequences determined by mass spectrophotometry. Proteins were identified by Mascot, using the Swiss Prot database. The results show that the motility (immature; 26.1±1.75% total motile cells vs. mature; 60.93±3.24% total motile cells; p<0.001) and morphology parameters (immature; 64.1±2.75% normal head morphology vs. mature; 87.63±3.24% normal head morphology; p<0.001) of the two populations differed significantly. After 2D electrophoresis, 16 differentially expressed protein spots were identified within the total sperm proteome between the immature and mature sperm populations. 56% of the differentially expressed proteins were more abundant in the immature sperm population compared to the mature sperm population. Functions have been ascribed to these proteins of which only four proteins, namely Tubulin -3C/D chain, Tubulin -2C chain, Outer dense fibre protein 2 and A-Kinase anchoring protein 4 precursor, were directly related to sperm motility and morphology. In the second part of the study the expression of nuclear proteins in human spermatozoa was investigated between immature and mature sperm populations. Semen was collected from healthy donors (n=156) and further separated from the seminal plasma by PureSperm® gradient centrifugation. The immature and mature sperm populations were retrieved and used during further analysis. For the proteomic analysis of nuclear proteins, cells were fractionated into four different subcellular protein fractions, instead of analyzing the whole sperm proteome. The results show that the motility (immature; 32.33±0.51% total motile cells vs. mature; 88.67±0.85% total motile cells; p<0.0001) and morphology parameters (immature; 13.51±0.87% normal head morphology vs. mature; 20.89±1.20% normal head morphology; p<0.0001) of the two populations differ significantly. After 2D electrophoresis, 21 differentially expressed nuclear proteins were identified between the immature and mature sperm populations. 95% of the differentially expressed nuclear proteins were less abundant in the immature population compared to the mature population. Only one nuclear protein namely 78kDa Glucose regulated protein was more abundant in the immature population compared to the mature population. Functions ascribed to these individual proteins were directly related to sperm motility, morphology and energy metabolism. In conclusion,In conclusion, in the current study novel techniques have been employed to investigate protein differences between immature and mature sperm populations. From these results it is evident that protein expression in the total sperm proteome and nuclear protein fraction is significantly different and incomplete in the immature population, compared to mature population. Based on these findings, it is recommended that further studies should be done on human spermatozoa to validate the role of the individual proteins in sperm function. Proteomics is an ideal tool to identify idiopathic causes of male infertility, as it can help to identify novel receptors (and signal transduction pathways) that can be used in the screening of drugs to alleviate sperm dysfunction.
AFRIKAANSE OPSOMMING: Manlike infertiliteit word dikwels geassosieer met verlaagde sperm motiliteit en morfologie (asthenoteratozoospermia) waarvoor daar tot dusver nog geen spesifieke terapeutiese behandeling is nie. Dit het aan die lig gekom dat die modifisering en uitdrukking van menslike sperm proteïene ‘n belangrike rol speel in spermfunksie. In die huidige studie stel ons data voor van proteiene in menslike sperme in die konteks van abnormale spermfunksie. Unieke tegnieke was gebruik om verskille in proteïen uitdrukking op sellulêre vlak suksesvol te isoleer en identifiseer wat verband hou met asthenoteratozoospermia. Tydens die eerste deel van die studie was verskille in proteïen uitdrukking binne die totale spermproteoom tussen onvolwasse en volwasse spermpopulasies ondersoek. Sperme van gesonde skenkers (n=23) is geskei in twee spermpopulasies (onvolwasse en volwasse sperme) deur middel van ‘n 3-laag Percoll gradiënt sentrifugasie tegniek. Selle is gewas en sperm motiliteit en morfologie is gemeet deur rekenaar geassisteerde sperm analise (CASA). Vir proteomiese analise is selle geliseer en proteïene geskei deur twee dimensionele gel elektroforese (2D-elektroforese). PD-Quest sagteware is gebruik om statisties beduidende proteïen verskille aan te dui. Die proteïene van belang is uitgesny en onderwerp aan in-gel vertering. Peptiede is geskei met behulp van hoë druk vloeistof chromatografie (HPLC) analise en aminosuurvolgordes is bepaal deur massa spektrofotometrie. Proteïene is geïdentifiseer met behulp van Mascot deur van die Swiss Prot databasis gebruik te maak. Die resultate toon dat die sperm motiliteit (onvolwasse; 26.1±1.75% totale motiele selle vs. volwasse; 60.93±3.24% totale motiele selle; p <0,001) en morfologiese parameters (onvolwasse; 64.1±2.75% normale kop morfologie vs. volwasse; 87.63±3.24% normale kop morfologie; p <0,001) tussen die twee populasies beduidend verskil. Na 2Delektroforese is 16 proteïen kolle geïdentifiseer wat beduidend verskil het, tussen die totale sperm proteoom van onvolwasse spermpopulasies en volwasse spermpopulasies. 56% van die proteïene wat beduidend verskil het, was meer uitgedruk in die onvolwasse spermpopulasie ten opsigte van die volwasse sperm populasie. Funksies is toegeskryf aan hierdie proteïene waarvan net vier proteïene naamlik Tubulin -3C/D ketting, Tubulin -2C ketting, Buite digte vesel proteïen 2 en A-Kinase anker proteïen 4 voorloper direk verband hou met sperm motiliteit en morfologie. In die tweede deel van die studie is die uitdrukking van nukluêre proteïene in menslike spermatozoa tussen onvolwasse en volwasse spermpopulasies ondersoek. Sperme was van gesonde skenkers (n=156) versamel en verder geskei van seminale plasma deur middel van ‘n PureSperm® gradiënt sentrifugasie tegniek. Vir die proteomiese analise van nukluêre proteïene is selle gefraksioneer in vier verskillende sub-sellulêre proteïen fraksies, in plaas van analise van die totale spermproteoom. Die resultate toon aan dat die sperm motiliteit (onvolwasse; 32.33±0.51% totale motiele selle vs. volwasse; 88.67±0.85% totale motiele selle; p <0,001) en morfologiese parameters (onvolwasse; 13.51±0.87% normale kop morfologie vs. volwasse; 20.89±1.20% normale kop morfologie; p <0,001) tussen die twee populasies beduidend verskil. Na 2D-elektroforese is 21 kern proteïen kolle geïdentifiseer wat betekenisvol uitgedruk was tussen onvolwasse en volwasse spermpopulasies. 95% van die nukluêre proteïene wat beduidend verskil het, was minder uitgedruk in die onvolwasse spermpopulasie ten opsigte van die volwasse spermpopulasie. Slegs een kern proteïen naamlik 78kDa Glukose gereguleerde proteïen was meer uitgedruk in die onvolwasse spermpopulasie in vergelyking met die volwasse spermpopulasie. Funksies is toegeskryf aan hierdie proteïene wat direk verband hou met sperm motiliteit, morfologie en energie metabolisme. Ten slotte, in die huidige studie is unieke tegnieke geïmplementeer om proteïen verskille tussen onvolwasse en volwasse spermpopulasies te ondersoek. Uit hierdie resultate is dit duidelik dat proteïen uitdrukking in die totale sperm proteoom en in die kern proteïen fraksie beduidend verskil en onvolledig is in die onvolwasse spermpopulasie ten opsigte van die volwasse spermpopulasie. Op grond van hierdie bevindinge word aanbeveel dat verdere studies op menslike sperme gedoen moet word ten einde die rol van individuele proteïene in sperm funksie te kan bepaal. Proteomika is ‘n ideale tegniek om die iodiopatiese oorsake van manlike infertiliteit te identifiseer, aangesien dit kan help in die identifisering van unieke reseptore (en seintransduksie paaie) wat gebruik kan word om sperm disfunksie te verbeter deur farmaseutiese behandeling.
MITONAKA, Tomoaki, Yoshiyuki MURAMATSU, Shin SUGIYAMA, Tomoaki MIZUNO und Yasuyoshi NISHIDA. „Essential roles of myosin phosphatase in the maintenance of epithelial cell integrity of Drosophila imaginal disc cells“. Elsevier, 2007. http://hdl.handle.net/2237/9388.
Bieber, Adrienne. „Effects of chemotherapeutic agents for testicular cancer on male rat reproductive organs and spermatozoal numbers, motility, and morphology“. Thesis, McGill University, 2005. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=83965.
McAlister, Debra Ann. „A comparison of motility and head morphology of sperm using different semen processing methods and three different staining techniques“. Thesis, Stellenbosch : University of Stellenbosch, 2010. http://hdl.handle.net/10019.1/5137.
Bibliography
ENGLISH ABSTRACT: Sperm morphology remains an important parameter in the prediction of fertility, both in vivo and in vitro. However, there remains a considerable level of concern surrounding the true potential of this parameter due to the lack of standardization of differential staining techniques used for the evaluation of sperm morphology. This study aimed at investigating two commonly used staining techniques, Rapidiff® (RD) and Papanicolaou (PAP), along with a new commercially available stain, SpermBlue® (SB), in the evaluation of sperm morphometry and morphology. Results indicated that significant differences in sperm morphometry exist due to the use of the staining techniques. Findings further indicated that RD causes sperm head swelling while PAP causes sperm head shrinkage. Results obtained using the SB staining technique have indicated measurements closest to that which would be obtained through the evaluation of fresh, unstained sperm. The lack of standardization and the different effects various stains have on sperm structure and overall sperm morphology evaluation should raise a level of concern, particularly when evaluating patients with borderline morphology. Based on this, the use of the SB staining technique is recommended over RD and PAP for effective and accurate morphology evaluation. In further support of this technique, SB was shown to be quick and simple in method, and allowed for the easy detection of sperm by computer aided sperm analysis (CASA) systems such as the Sperm Class Analyzer (SCA®). The second aim of this study was to examine the concentration, morphology and motility of the resultant sperm populations following semen preparation using the PureSperm® density gradient and swim-up techniques. Semen preparation is an essential step in any fertility treatment protocol, and it is important that the sperm obtained following semen preparation has sperm morphology and motility characteristics capable of improving assisted fertility success rates. Currently, the PureSperm® density gradient and sperm swim-up are the most widely employed techniques in fertility clinics. Although there is sufficient evidence to suggest they are each effective at extracting sperm with improved quality from neat semen, there remains insufficient evidence to suggest which of these two techniques is superior. The present investigation revealed that both sperm preparation methods were effective at improving sperm morphology and motility, however to varying degrees. The swimup method yielded a population of sperm with superior motility and morphology when assessed according to World Health Organisation (WHO) criteria, while the PureSperm® density gradient technique isolated a higher percentage of normal sperm, according to both WHO and Tygerberg strict criteria, with motility better than that of neat semen. Although results obtained via the swim-up method suggest it would be best for use in in vitro fertilization (IVF), the very low concentration of sperm isolated via this method remains a significant draw-back. The PureSperm® density gradient separation technique on the other hand is capable of isolating larger quantities of sperm, which is likely to be of more benefit with fertility treatments requiring larger quantities of sperm. Based on these findings, the use of PureSperm® density gradient technique is recommended, due to its ability to isolate large quantities of good quality sperm. However, a swim-up may still be of use when performing fertility treatment using a sperm sample which possesses a high concentration and motility.
AFRIKAANSE OPSOMMING: Sperm morfologie bly ‘n belangrike parameter in die voorspelling van vrugbaarheid, beide in vivo en in vitro. Tog is daar nogsteeds ‘n aansienklike vlak van kommer rondom die ware potensiaal van hierdie parameter weens die gebrek aan standardisering van verskillende kleuringstegnieke wat gebruik word vir die evaluering van spermmorfologie. Hierdie studie is daarop gemik om ondersoek in te stel na twee algemeen gebruikte kleurings tegnieke naamlik, Rapidiff® (RD) en Papanicolaou (PAP), asook ‘n nuwe kommersiëel beskikbare kleurstof, SpermBlue® (SB), vir die evaluering van spermmorfometrie en morfologie. Resultate dui aan dat beduidende verskille in sperm morfometriese afmetings ontstaan as gevolg van die gebruik van die verskillende kleurstowwe. Bevindinge dui verder daarop dat RD swelling van die sperm se kop versoorsaak, terwyl PAP die spermkop laat krimp. Resultate wat verkry is met behulp van die SB kleuringstegniek dui daarop dat hierdie kleurstof aanleiding gegee het tot afmetings naaste aan die verkry tydens die beoordeling van vars, ongekleurde sperme. Die gebrek aan standardisasie en die uiteenlopende effekte wat verskillende kleurstowwe het op die spermstruktuur en die evaluering van sperm morfologie ingeheel is kommerwekkend, veral tydens die evaluering van pasiënte met grensgeval morfologie. Op grond van hierdie resultate, word die gebruik van die SB kleuringstegniek, bo die gebruik van RD en PAP, vir effektiewe en akkurate morfologie evaluering aanbeveel. Verdere ondersteuning vir die gebruik van die SB kleuringstegniek is die feit dat daar bevind is dat SB ‘n vinnige en eenvoudige metode is, wat toelaat vir maklike visualisering van sperme deur rekenaargesteunde sperm analise sisteme soos die Sperm Class Analyzer (SCA®). Die tweede doel van hierdie studie was om die konsentrasie, morfologie en die motiliteit van spermpopulasies te ondersoek, soos verkry tydens die voorbereiding van semen deur gebruik te maak van die PureSperm® digtheidsgradiënt en op-swem tegnieke. Die voorbereiding van semen is ‘n noodsaaklike stap in enige vrugbaarheidsbehandeling protokol, aangesien dit belangrik is dat die sperme wat deur hierdie prosesse verkry word oor die nodige morfologiese en motiliteit eienskappe beskik wat in staat is om die sukses van vrugbaarheidsbehandelings te verbeter. Huidiglik is die PureSperm® digtheidsgradiënt en op-swem tegnieke die mees algemeen gebruikte tegnieke in vrugbaarheidsklinieke. Alhoewel daar voldoende bewyse is wat voorstel dat elke tegniek effektief is vir die ekstraksie van sperme met beter kwaliteit vanuit semen, bly daar steeds onvoldoende bewyse wat daarop dui dat een van hierdie twee tegnieke beter is as die ander een. Huidige navorsing het getoon dat beide sperm voorbereidings metodes daarin geslaag het om sperme met normale morfologie en beter motiliteit te selekteer. Die opswem metode het ‘n spermpopulasie met beter motiliteit en verbeterde morfologie gelewer, soos getoets volgens die WGO kriteria, terwyl die PureSperm digtheidsgradiënt tegniek sperme met verbeterde morfologie, volgens beide die WGO en Tygerberg Streng Kriteria, en ‘n redelike verbetering in sommige motiliteits parameters geselekteer het. Hoewel die resultate wat verkry word via die op-swem metode voorstel dat dit die beste metode vir die gebruik tydens in vitro bevrugting sou wees, bly die baie lae konsentrasie van sperme wat met hierdie metode verkry word ‘n belangrike nadeel. Die PureSperm® skeidingstegniek laat egter toe vir die isolering van groter hoeveelhede sperme, wat waarskynlik meer voordelig sal wees vir bevrugtingsbehandelings wat meer sperme benodig. Gebaseer op hierdie bevindinge, word die gebruik van die PureSperm® digtheidsgradiënt tegniek aanbeveel, as gevolg van hierdie tegniek se vermoë om groot hoeveelhede goeie gehalte sperm te isoleer. Daar kan egter nogsteeds van op-swem metodes gebruik gemaak word tydens vrugbaarheidsbehandeling indien die semenmonster beskik oor ‘n hoë konsentrasie sperme met goeie beweeglikheid.
Spreng, Benjamin Roman [Verfasser], und Friedrich [Akademischer Betreuer] Frischknecht. „The role of microtubules in Plasmodium berghei sporozoite development, morphology, motility and infectivity / Benjamin Roman Spreng ; Betreuer: Friedrich Frischknecht“. Heidelberg : Universitätsbibliothek Heidelberg, 2019. http://d-nb.info/1197235671/34.
Bücher zum Thema "Motility and morphology":
Abercrombie Conference (2nd 1987 Oxford, England). Cell behaviour: Shape, adhesion and motility : the second Abercrombie Conference : proceedings of the British Society for Cell Biology-The Company of Biologists Limited symposium, Oxford, April 1987. Cambridge: Company of Biologists, 1987.
Soderquist, Lennart. Sperm characteristics and fertility in dairy A.I. bulls: With special reference to sperm motility, ATP content, sperm morphology, and spermatogenesis. Uppsala: Sveriges Lantbruksuniversitet, 1991.
Zürcher Workshop on Cell Traffic in the Developing and Adult Organism (1984). Motility of vertebrate cells in culture and in the organism: Molecular mechanisms and morphologic manifestations. Herausgegeben von Haemmerli Gisela 1923- und Sträuli Peter 1918-. Basel ; New York: Karger, 1985.
Gregory, Bock, Goode Jamie, Singapore. Institute of Molecular and Cell Biology. und Novartis Foundation Symposium on Signalling Networks in Cell Shape and Motility (2004 : Singapore), Hrsg. Signalling networks in cell shape and motility. New York: Wiley, 2005.
Cell behaviour: Shape, adhesion and motility : the second Abercrombie Conference : Proceedings of the British Society for Cell Biology-The Company of Biologists ... Oxford, April 1987 (Journal of cell science). Company of Biologists, 1987.
E, Jones Gareth, Wigley C. B, Warn Richard und Society for Experimental Biology (Great Britain), Hrsg. Cell behaviour: Adhesion and motility. Cambridge, UK: Published for the Society for Experimental Biology by the Company of Biologists Limited, Department of Zoology, University of Cambridge, 1993.
Jones, G. E. Cell Behaviour: Adhesion and Motility (Society for Experimental Biology). Society for Experimental Biology, 1993.
Buchteile zum Thema "Motility and morphology":
Alavi, Sayyed Mohammad Hadi, Andrzej Ciereszko, Azadeh Hatef, Jiří Křišťan, Boris Dzyuba, Sergei Boryshpolets, Marek Rodina, Jacky Cosson und Otomar Linhart. „Sperm Morphology, Physiology, Motility, and Cryopreservation in Percidae“. In Biology and Culture of Percid Fishes, 163–91. Dordrecht: Springer Netherlands, 2015. http://dx.doi.org/10.1007/978-94-017-7227-3_5.
Maggavi, Raghavendra, Sanjay Pujari und C. N. Vijaykumar. „Analysis of Cell Morphology, Vitality, and Motility: A Study Related to Analysis of Human Sperm“. In Proceedings of International Conference on Computational Intelligence and Data Engineering, 11–17. Singapore: Springer Singapore, 2019. http://dx.doi.org/10.1007/978-981-13-6459-4_2.
Dondero, Franco, Andrea Lenzi und Loredana Gandini. „Semen analysis“. In Oxford Textbook of Endocrinology and Diabetes, 1368–73. Oxford University Press, 2011. http://dx.doi.org/10.1093/med/9780199235292.003.9041.
Nanna, Abimibola. „Methods of Sperm Selection for In-Vitro Fertilization“. In Male Reproductive Anatomy [Working Title]. IntechOpen, 2021. http://dx.doi.org/10.5772/intechopen.99874.
Konferenzberichte zum Thema "Motility and morphology":
Sheng Dai, Chang, Zhuoran Zhang, James Huang, Xian Wang, Wenlong Meng, Junyan Zhang, Sergey Moskovtsev, Clifford Librach, Keith Jarvi und Yu Sun. „Automated Non-Invasive Measurement of Sperm Motility and Morphology Parameters“. In 2018 IEEE International Conference on Robotics and Automation (ICRA). IEEE, 2018. http://dx.doi.org/10.1109/icra.2018.8461252.
Thangawng, Abel L., Rodney S. Ruoff, Jonathan C. Jones und Matthew R. Glucksberg. „Substrate Stiffness Affects Laminin-332 Matrix Deposition in Cultured Keretinocytes“. In ASME 2007 Summer Bioengineering Conference. American Society of Mechanical Engineers, 2007. http://dx.doi.org/10.1115/sbc2007-176292.
Chen, W. C., P. C. Hsieh, G. M. Wang und M. L. Yeh. „The influence of surface morphology and rigidity of the substrata on cell motility“. In 2009 IEEE 35th Annual Northeast Bioengineering Conference. IEEE, 2009. http://dx.doi.org/10.1109/nebc.2009.4967790.
Choi, Chang K., Chuck Margraves, Anthony E. English und Kenneth D. Kihm. „Opto-Electric Biosensor to Examine In Vitro Toxicity Stimuli to Endothelial Cell Motility and Morphology“. In ASME 2007 Summer Bioengineering Conference. American Society of Mechanical Engineers, 2007. http://dx.doi.org/10.1115/sbc2007-176715.
Regis, Shawn, Manisha Jassal, Sina Youssefian, Nima Rahbar und Sankha Bhowmick. „Quantitative Studies of Fibronectin Adsorption on Submicron Scaffolds“. In ASME 2012 Summer Bioengineering Conference. American Society of Mechanical Engineers, 2012. http://dx.doi.org/10.1115/sbc2012-80633.
Schafer, Anette C., Meghan Discoll, Ashwathi Mohan, Andrew Ludlow, Wesley Burford, Jerry W. Shay und Gaudenz Danuser. „Abstract 5102: Differential levels of mutated Kras drive lung cancer cell motility and morphology via a hypoxia-sensitive FAK/Erk/Myosin II signaling pathway“. In Proceedings: AACR 106th Annual Meeting 2015; April 18-22, 2015; Philadelphia, PA. American Association for Cancer Research, 2015. http://dx.doi.org/10.1158/1538-7445.am2015-5102.
Jetta, Deekshitha, Deepika Verma, Mohammad M. Maneshi und Susan Z. Hua. „Shear Stress Induced Calcium Dependent Nuclear Deformation in Epithelial Cells“. In ASME 2018 International Mechanical Engineering Congress and Exposition. American Society of Mechanical Engineers, 2018. http://dx.doi.org/10.1115/imece2018-87650.
Masuda, Michiaka, und Keigi Fujiwara. „Three Distinct Types of Morphological Responses of Cultured Vascular Endothelial Cells to Physiological Levels of Fluid Shear Stress“. In ASME 2003 1st International Conference on Microchannels and Minichannels. ASMEDC, 2003. http://dx.doi.org/10.1115/icmm2003-1124.
Berichte der Organisationen zum Thema "Motility and morphology":
Azios, Nicolas G., und Surangani Dharawardhane. Phytoestrogen Effects on Cytoskeletal Morphology and Motility in Breast Cancer Progression. Fort Belvoir, VA: Defense Technical Information Center, März 2005. http://dx.doi.org/10.21236/ada434058.